We recommend setting up 10 ml of PCR master mix and aliquoting it into a 96-well plate, 100 µl per well. This protocol is scaled for a starting amount of 10 ml; simply scale the volumes in the later sections accordingly if you start with a different scale of PCR. In our hands, this typically results in a final yield of 500 – 2000 nmole of ssDNA FISH probe.
- 200 µM unlabeled “R” primer stock
- 200 µM labeled “F” primer stock