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Build XCalibur Inclusion List
# BUILD THERMO XCALIBUR INCLUSION LIST
# returns inclusion list dataframe
build_inclusion_list <- function(
input, # dataframe, with columns:
# `Raw file`, `Modified sequence`, `Retention time`, `Charge`
file='inclusion_list.csv', # output file
rt_width=3, # width, in minutes, of the RT window on either side
mz_decimals=5, # number of decimals to round m/z to
rt_decimals=1, # number of decimals to round RT to
transform_rt=NA, # number (RT = RT + x) or function ( RT = f(RT) ) to transform RTs
rt_alignment=FALSE, # perform RT alignment? (simple linear model)
rt_alignment_reference=NA, # name of the raw file to align to (reference experiment).
# if NA, then use last experiment as reference.
TMT=TRUE, # is this a TMT experiment?
polarity='Positive' # positive or negative ionization mode?
) {
library(tidyverse)
library(stringr)
# element monoisotopic masses
element_mass <- c(
H=1.0078250321,
D=2.014101779,
C=12.0,
Cx=13.0033548378,
N=14.0030740052,
Nx=15.0001088984,
O=15.9949146221,
Ox=17.9991603,
F=18.99840320,
P=30.97376151,
S=31.97207069,
Cl=34.96885271,
Na=22.98976967,
K=38.9637069,
Ca=39.9625912,
Fe=53.9396148
)
# amino acid monoisotopic masses
aa_mass = c(
A=71.0371137878,
C=103.0091844778,
D=115.026943032,
E=129.0425930962,
F=147.0684139162,
G=57.0214637236,
H=137.0589118624,
I=113.0840639804,
K=128.0949630177,
L=113.0840639804,
M=131.0404846062,
N=114.0429274472,
P=97.052763852,
Q=128.0585775114,
R=156.1011110281,
S=87.0320284099,
T=101.0476784741,
U=150.95363, # Selenocysteine
V=99.0684139162,
W=186.0793129535,
Y=163.0633285383,
X=113.0840639804 # Xle - Isoleucine or Leucine
)
mods <- c(
`p`=79.9663304084, # phosphorylation (HPO3)
`_(ac)`=42.0105646863, # n-terminal acetylation (C2H2O)
`(ox)`=15.9949146221, # methionine oxidation (O)
`(ar)`=10.0082685996, # superheavy arginine (Cx6Nx4) - (C6N4)
`(ly)`=8.01419881320 # superheavy lysine (Cx6Nx2) - (C6N2)
)
proton_mass = 1.0072764666
electron_mass = 0.0005485799
water_mass = 18.0105646863
tmt_mass = 229.162932141
# Perform RT alignment? ---------------------------------------------------
input$RT_aligned <- input$`Retention time`
if(rt_alignment) {
# if no reference experiment given, use the last one as the reference
ref_exp_name <- NA
if(is.na(rt_alignment_reference)) {
ref_exp_name <- last(sort(unique(input$`Raw file`)))
} else {
ref_exp_name <- rt_alignment_reference
}
# pull reference experiment data from input dataframe
ref_exp <- input %>%
filter(grepl(ref_exp_name, `Raw file`)) %>%
distinct(`Raw file`, `Modified sequence`, .keep_all=T) %>%
arrange(`Modified sequence`)
input_aligned <- data.frame()
for(exp in exps) {
e <- input %>%
filter(`Raw file` == exp) %>%
distinct(`Raw file`, `Modified sequence`, .keep_all=T) %>%
arrange(`Modified sequence`)
if(exp != ref_exp_name) {
# find common peptides
common_peps <- intersect(ref_exp$`Modified sequence`, e$`Modified sequence`)
# linear alignment
y <- e %>% filter(`Modified sequence` %in% common_peps) %>% pull(`Retention time`)
x <- ref_exp %>% filter(`Modified sequence` %in% common_peps) %>% pull(`Retention time`)
fit <- lm(y ~ x)
e$RT_aligned <- (e$`Retention time` - fit$coefficients[1]) / fit$coefficients[2]
}
input_aligned <- rbind(input_aligned, e)
}
input <- input_aligned
}
# Begin building inclusion list dataframe ---------------------------------
inc_list <- input %>%
mutate(Sequence=gsub('([^A-Z])', '', `Modified sequence`)) %>% # derive unmodified AA sequence
group_by(`Modified sequence`) %>%
summarise(RT=mean(RT_aligned),
#RT_spread=max(RT_aligned)-min(RT_aligned),
Charge=first(Charge),
Sequence=first(Sequence))
# Recalculate peptide mass ------------------------------------------------
inc_list$Mass <- sapply(inc_list$`Modified sequence`, function(s) {
mass <- 0.0
# Add amino acid masses
for(i in 1:nchar(s)) {
char <- substr(s, i, i)
if(char %in% names(aa_mass)) { mass <- mass + aa_mass[char] }
}
# Add modification masses
for(mod in names(mods)) {
mod_count <- str_count(s, mod)
mass <- mass + (mod_count * mods[mod])
}
if(TMT) {
# add a TMT for each lysine
mass <- mass + (str_count(s, 'K') * tmt_mass)
# add a TMT if the n-terminus is not acetylated
if(!grepl('_(ac)', s)) {
mass <- mass + tmt_mass
}
}
# add a water
mass <- mass + water_mass
})
# Calculate M+H and m/z
if(polarity == 'Positive') {
inc_list$`M+H` <- inc_list$Mass + (inc_list$Charge * proton_mass)
}
inc_list$`m/z` <- inc_list$`M+H` / inc_list$Charge
# Apply RT Transform? -----------------------------------------------------
if(!is.na(transform_rt)) {
if(class(transform_rt) == 'numeric' | class(transform_rt) == 'integer') {
inc_list$RT <- inc_list$RT + transform_RT
} else if (class(transform_rt) == 'function') {
inc_list$RT <- transform_rt(inc_list$RT)
} else {
stop('Argument "transform_rt" has invalid type. "transform_rt" needs to be an integer, numeric, or a function f(RT) that takes in an RT and returns an RT.')
}
}
# Final Data Wrangling ----------------------------------------------------
# collapse duplicate ions, wrangle columns
inc_list <- inc_list %>%
group_by(Sequence, `m/z`) %>%
summarise(RT=mean(RT), Charge=first(Charge)) %>%
ungroup() %>%
mutate(
`Mass [m/z]`=round(`m/z`, mz_decimals),
`Formula [M]`=NA, `Formula type`=NA, `Species`=NA,
`CS [z]`=Charge,
`Polarity`=polarity,
`Start [min]`=round(RT - rt_width, rt_decimals),
`End [min]`=round(RT + rt_width, rt_decimals),
`(N)CE`=NA, `(N)CE type`=NA, `MSX ID`=NA, `Comment`=NA
) %>%
select(-c('Sequence', 'Charge', 'm/z', 'RT')) %>%
arrange(`Start [min]`)
# Save file ---------------------------------------------------------------
write_csv(inc_list, path=file, na='')
return(inc_list)
}
Mass [m/z] Formula [M] Formula type Species CS [z] Polarity Start [min] End [min] (N)CE (N)CE type MSX ID Comment
534.56487 3 Positive 19.6 25.6
642.79278 2 Positive 25.3 31.3
599.91709 3 Positive 27.6 33.6
790.81475 2 Positive 27.9 33.9
667.79447 2 Positive 28.1 34.1
626.57253 3 Positive 28.4 34.4
510.23666 3 Positive 28.4 34.4
465.54695 3 Positive 28.7 34.7
616.2795 2 Positive 28.8 34.8
599.93805 3 Positive 29.1 35.1
676.29568 2 Positive 29.2 35.2
656.26267 2 Positive 29.5 35.5
558.25021 2 Positive 29.8 35.8
587.82402 2 Positive 29.8 35.8
542.23711 2 Positive 29.9 35.9
739.85265 2 Positive 29.9 35.9
534.92816 3 Positive 29.9 35.9
474.73132 4 Positive 30.1 36.1
592.93464 3 Positive 30.2 36.2
501.90591 3 Positive 30.3 36.3
684.76134 2 Positive 30.4 36.4
519.90513 3 Positive 30.5 36.5
586.93012 3 Positive 30.5 36.5
589.57752 3 Positive 30.7 36.7
466.54783 3 Positive 30.7 36.7
502.22575 4 Positive 31 37
589.74391 2 Positive 31 37
543.20658 3 Positive 31.2 37.2
512.58131 3 Positive 31.3 37.3
735.97897 3 Positive 31.4 37.4
562.76857 2 Positive 31.5 37.5
599.78696 2 Positive 31.5 37.5
702.98925 3 Positive 31.8 37.8
514.23666 3 Positive 32 38
794.8578 2 Positive 32.2 38.2
476.93613 4 Positive 32.3 38.3
630.24744 3 Positive 32.3 38.3
883.86265 2 Positive 32.4 38.4
530.24096 3 Positive 32.5 38.5
573.2826 3 Positive 32.7 38.7
808.3515 2 Positive 32.8 38.8
518.75493 2 Positive 32.9 38.9
646.281 2 Positive 32.9 38.9
588.78984 2 Positive 33 39
580.26482 2 Positive 33 39
650.27029 2 Positive 33 39
639.77013 2 Positive 33.1 39.1
626.30585 2 Positive 33.3 39.3
657.30193 2 Positive 33.4 39.4
474.55488 3 Positive 33.5 39.5
513.91804 3 Positive 33.5 39.5
454.88367 3 Positive 33.5 39.5
628.27914 3 Positive 33.8 39.8
555.73694 2 Positive 34.1 40.1
872.37285 2 Positive 34.2 40.2
572.28967 2 Positive 34.2 40.2
558.49529 4 Positive 34.3 40.3
783.87887 2 Positive 34.3 40.3
484.90736 3 Positive 34.4 40.4
489.57924 3 Positive 34.4 40.4
891.65628 3 Positive 34.6 40.6
477.50091 4 Positive 34.7 40.7
519.74457 2 Positive 34.8 40.8
536.26544 3 Positive 34.8 40.8
639.93726 3 Positive 34.9 40.9
507.28853 3 Positive 34.9 40.9
801.86562 2 Positive 35 41
762.63441 3 Positive 35 41
744.32463 3 Positive 35.1 41.1
486.73928 2 Positive 35.3 41.3
505.74711 2 Positive 35.5 41.5
768.81189 2 Positive 35.5 41.5
646.80176 2 Positive 35.5 41.5
583.77386 2 Positive 35.6 41.6
527.26455 3 Positive 35.6 41.6
535.57259 3 Positive 35.7 41.7
629.63274 3 Positive 35.8 41.8
460.94872 4 Positive 35.8 41.8
555.59551 3 Positive 35.8 41.8
532.7762 2 Positive 35.8 41.8
608.25492 3 Positive 35.9 41.9
579.94214 3 Positive 35.9 41.9
663.30605 2 Positive 35.9 41.9
574.24101 2 Positive 36 42
525.2532 3 Positive 36 42
548.7655 2 Positive 36.1 42.1
538.50371 4 Positive 36.2 42.2
573.61792 3 Positive 36.2 42.2
517.90591 3 Positive 36.2 42.2
526.59267 3 Positive 36.3 42.3
765.36305 2 Positive 36.3 42.3
606.28459 2 Positive 36.3 42.3
520.94034 3 Positive 36.3 42.3
683.57484 4 Positive 36.4 42.4
655.29684 2 Positive 36.4 42.4
608.30024 2 Positive 36.5 42.5
528.58406 3 Positive 36.6 42.6
787.35235 2 Positive 36.7 42.7
462.23096 3 Positive 36.8 42.8
979.91253 2 Positive 36.8 42.8
623.77933 2 Positive 36.8 42.8
465.25835 3 Positive 36.8 42.8
697.83498 2 Positive 36.9 42.9
533.94397 3 Positive 36.9 42.9
539.26821 2 Positive 36.9 42.9
549.91295 3 Positive 36.9 42.9
519.59558 3 Positive 37 43
594.61854 3 Positive 37 43
716.29704 2 Positive 37 43
516.9209 3 Positive 37 43
628.7912 2 Positive 37 43
461.88433 3 Positive 37 43
470.88797 3 Positive 37.1 43.1
525.23732 3 Positive 37.1 43.1
543.57634 3 Positive 37.2 43.2
646.26775 2 Positive 37.2 43.2
622.27662 3 Positive 37.2 43.2
646.6205 3 Positive 37.2 43.2
633.54736 4 Positive 37.5 43.5
506.91363 3 Positive 37.5 43.5
852.03352 3 Positive 37.5 43.5
453.5712 3 Positive 37.6 43.6
593.27115 2 Positive 37.7 43.7
627.96935 3 Positive 37.7 43.7
507.59764 3 Positive 37.8 43.8
611.95292 3 Positive 37.9 43.9
539.2508 3 Positive 37.9 43.9
671.99295 3 Positive 37.9 43.9
666.79278 2 Positive 38 44
741.68702 3 Positive 38 44
745.68959 3 Positive 38 44
847.67075 3 Positive 38.1 44.1
775.81972 2 Positive 38.1 44.1
553.91999 3 Positive 38.1 44.1
749.5844 4 Positive 38.2 44.2
546.61486 3 Positive 38.2 44.2
670.30826 2 Positive 38.2 44.2
588.2793 4 Positive 38.2 44.2
616.27512 3 Positive 38.2 44.2
654.93459 3 Positive 38.2 44.2
522.27981 3 Positive 38.3 44.3
609.77131 2 Positive 38.3 44.3
464.87936 3 Positive 38.3 44.3
506.9025 3 Positive 38.3 44.3
601.58965 3 Positive 38.4 44.4
725.81252 2 Positive 38.4 44.4
591.58259 3 Positive 38.5 44.5
510.49328 4 Positive 38.5 44.5
557.62301 3 Positive 38.5 44.5
686.3252 3 Positive 38.5 44.5
624.78865 2 Positive 38.5 44.5
559.63436 3 Positive 38.6 44.6
502.25188000000003 4 Positive 38.6 44.6
784.03665 3 Positive 38.7 44.7
699.33481 2 Positive 38.8 44.8
711.61317 3 Positive 38.9 44.9
725.84396 2 Positive 38.9 44.9
715.31947 2 Positive 38.9 44.9
556.61429 3 Positive 39 45
553.2532 3 Positive 39 45
519.61145 3 Positive 39 45
538.61486 3 Positive 39.1 45.1
608.58523 3 Positive 39.2 45.2
847.34 3 Positive 39.2 45.2
547.60334 3 Positive 39.2 45.2
679.31323 3 Positive 39.2 45.2
450.45685 4 Positive 39.3 45.3
521.60491 3 Positive 39.3 45.3
750.2782 3 Positive 39.4 45.4
786.84065 2 Positive 39.4 45.4
686.34035 3 Positive 39.5 45.5
635.75682 4 Positive 39.5 45.5
654.78865 2 Positive 39.5 45.5
528.75266 4 Positive 39.5 45.5
704.66778 3 Positive 39.5 45.5
590.95867 3 Positive 39.6 45.6
602.7691 2 Positive 39.6 45.6
479.24191 3 Positive 39.6 45.6
523.61145 3 Positive 39.7 45.7
582.25095 3 Positive 39.7 45.7
837.69248 3 Positive 39.8 45.8
567.76075 2 Positive 39.8 45.8
537.75513 2 Positive 39.8 45.8
627.82388 2 Positive 39.8 45.8
570.96678 3 Positive 39.9 45.9
619.61334 3 Positive 39.9 45.9
570.96678 3 Positive 39.9 45.9
634.83623 2 Positive 40 46
698.83718 2 Positive 40 46
738.0244 3 Positive 40 46
498.539 3 Positive 40.1 46.1
575.95728 3 Positive 40.1 46.1
740.99277 3 Positive 40.1 46.1
885.93437 2 Positive 40.1 46.1
538.28685 3 Positive 40.1 46.1
490.22313 3 Positive 40.2 46.2
734.83105 2 Positive 40.2 46.2
484.21077 3 Positive 40.2 46.2
701.00569 3 Positive 40.3 46.3
630.29419 4 Positive 40.3 46.3
761.86426 2 Positive 40.3 46.3
542.95874 3 Positive 40.3 46.3
549.93514 3 Positive 40.3 46.3
583.59551 3 Positive 40.3 46.3
708.34462 2 Positive 40.4 46.4
690.76134 2 Positive 40.4 46.4
644.28373 2 Positive 40.4 46.4
803.4176 2 Positive 40.5 46.5
587.32553 3 Positive 40.5 46.5
583.6166 3 Positive 40.6 46.6
578.28881 3 Positive 40.6 46.6
698.34518 2 Positive 40.6 46.6
554.74257 4 Positive 40.6 46.6
710.29142 2 Positive 40.6 46.6
643.62038 3 Positive 40.6 46.6
866.92958 2 Positive 40.7 46.7
702.29345 3 Positive 40.7 46.7
696.36276 3 Positive 40.7 46.7
711.7073 3 Positive 40.7 46.7
881.91255 2 Positive 40.8 46.8
532.75013 4 Positive 40.8 46.8
479.5906 3 Positive 40.8 46.8
556.27523 3 Positive 40.9 46.9
572.61911 3 Positive 40.9 46.9
580.62917 3 Positive 40.9 46.9
686.56845 4 Positive 41 47
692.28498 2 Positive 41 47
667.63319 3 Positive 41 47
618.58191 3 Positive 41 47
694.57104 4 Positive 41 47
553.77012 4 Positive 41 47
597.61693 3 Positive 41 47
683.3232 2 Positive 41.1 47.1
614.27231 2 Positive 41.1 47.1
534.03229 4 Positive 41.1 47.1
602.62486 3 Positive 41.2 47.2
829.37616 2 Positive 41.2 47.2
972.42492 3 Positive 41.3 47.3
772.34557 2 Positive 41.3 47.3
519.29557 3 Positive 41.3 47.3
520.27174 4 Positive 41.4 47.4
471.58685 3 Positive 41.4 47.4
815.87936 2 Positive 41.5 47.5
1002.41832 2 Positive 41.5 47.5
709.99774 3 Positive 41.5 47.5
535.26567 2 Positive 41.5 47.5
577.7383 2 Positive 41.5 47.5
769.99836 3 Positive 41.5 47.5
588.27746 3 Positive 41.6 47.6
548.93223 3 Positive 41.7 47.7
583.26974 3 Positive 41.7 47.7
613.98097 3 Positive 41.7 47.7
712.98064 3 Positive 41.7 47.7
503.56181 3 Positive 41.7 47.7
535.94749 3 Positive 41.8 47.8
593.96293 3 Positive 41.8 47.8
664.35479 2 Positive 41.8 47.8
511.61653 3 Positive 41.8 47.8
668.61464 3 Positive 41.9 47.9
460.58344 3 Positive 41.9 47.9
729.86572 2 Positive 41.9 47.9
754.83907 2 Positive 41.9 47.9
476.24046 4 Positive 41.9 47.9
684.98982 3 Positive 42 48
556.76857 2 Positive 42 48
767.64821 3 Positive 42 48
617.94678 3 Positive 42 48
653.32371 2 Positive 42 48
561.26312 2 Positive 42.1 48.1
750.80628 2 Positive 42.1 48.1
541.6271 3 Positive 42.2 48.2
789.33352 3 Positive 42.2 48.2
680.3527 3 Positive 42.2 48.2
541.61222 3 Positive 42.2 48.2
883.93381 2 Positive 42.2 48.2
613.65197 3 Positive 42.2 48.2
662.25992 2 Positive 42.3 48.3
572.7764 4 Positive 42.3 48.3
591.30457 3 Positive 42.3 48.3
550.26689 3 Positive 42.3 48.3
709.95555 3 Positive 42.3 48.3
673.99853 3 Positive 42.3 48.3
561.29401 3 Positive 42.4 48.4
705.64823 3 Positive 42.4 48.4
665.80959 2 Positive 42.5 48.5
694.9754 3 Positive 42.5 48.5
491.91379 3 Positive 42.5 48.5
792.03565 3 Positive 42.6 48.6
598.05123 4 Positive 42.6 48.6
594.30468 3 Positive 42.6 48.6
705.80179 4 Positive 42.7 48.7
783.31687 3 Positive 42.7 48.7
562.78677 2 Positive 42.7 48.7
492.60938 3 Positive 42.7 48.7
630.26349 3 Positive 42.7 48.7
799.99982 3 Positive 42.7 48.7
540.29546 3 Positive 42.7 48.7
589.30004 2 Positive 42.7 48.7
626.81605 2 Positive 42.8 48.8
873.99544 3 Positive 42.8 48.8
493.92514 3 Positive 42.8 48.8
829.35644 3 Positive 42.8 48.8
556.51976 4 Positive 42.8 48.8
603.6522 3 Positive 42.8 48.8
554.95465 3 Positive 42.8 48.8
589.77386 2 Positive 42.8 48.8
740.34582 3 Positive 42.8 48.8
550.76296 2 Positive 42.9 48.9
612.91423 3 Positive 42.9 48.9
660.78368 4 Positive 42.9 48.9
728.9489 3 Positive 43 49
736.65319 3 Positive 43 49
480.22665 3 Positive 43 49
719.83633 2 Positive 43 49
638.68538 5 Positive 43.1 49.1
731.57123 4 Positive 43.1 49.1
490.58188 3 Positive 43.1 49.1
712.99579 3 Positive 43.2 49.2
720.95164 3 Positive 43.2 49.2
564.2446 2 Positive 43.3 49.3
586.64482 3 Positive 43.3 49.3
577.76412 2 Positive 43.3 49.3
488.23448 3 Positive 43.4 49.4
731.84808 2 Positive 43.4 49.4
571.63866 3 Positive 43.4 49.4
588.98517 3 Positive 43.4 49.4
565.28948 2 Positive 43.4 49.4
795.63705 3 Positive 43.4 49.4
781.9784 3 Positive 43.4 49.4
687.61805 3 Positive 43.4 49.4
721.84554 2 Positive 43.5 49.5
467.24974 3 Positive 43.5 49.5
667.31504 2 Positive 43.5 49.5
925.66414 4 Positive 43.5 49.5
654.82916 2 Positive 43.6 49.6
498.75748 2 Positive 43.6 49.6
629.79753 2 Positive 43.6 49.6
647.79341 2 Positive 43.6 49.6
569.28316 3 Positive 43.6 49.6
620.97707 3 Positive 43.7 49.7
555.9745 3 Positive 43.7 49.7
470.57002 3 Positive 43.7 49.7
685.9907 3 Positive 43.7 49.7
757.31648 2 Positive 43.8 49.8
683.29216 3 Positive 43.8 49.8
563.29478 3 Positive 43.8 49.8
514.74484 4 Positive 43.8 49.8
732.82597 2 Positive 43.9 49.9
584.02367 4 Positive 43.9 49.9
844.67874 3 Positive 43.9 49.9
777.99032 3 Positive 43.9 49.9
607.98958 3 Positive 43.9 49.9
524.59546 3 Positive 43.9 49.9
500.57836 3 Positive 43.9 49.9
855.8985 2 Positive 44 50
904.41231 2 Positive 44 50
545.95102 3 Positive 44 50
479.57473 3 Positive 44 50
673.04522 4 Positive 44 50
518.93307 3 Positive 44.1 50.1
789.37212 2 Positive 44.1 50.1
819.39025 3 Positive 44.1 50.1
617.96376 3 Positive 44.1 50.1
902.40284 3 Positive 44.1 50.1
693.32593 2 Positive 44.1 50.1
698.8428 2 Positive 44.1 50.1
623.6234 3 Positive 44.1 50.1
481.56612 3 Positive 44.2 50.2
665.8319 2 Positive 44.2 50.2
770.29436 2 Positive 44.2 50.2
570.93476 3 Positive 44.2 50.2
616.26231 3 Positive 44.2 50.2
591.74338 2 Positive 44.2 50.2
731.32323 3 Positive 44.2 50.2
690.91337 3 Positive 44.2 50.2
580.06691 4 Positive 44.3 50.3
926.442 2 Positive 44.3 50.3
754.98746 3 Positive 44.3 50.3
538.92497 3 Positive 44.3 50.3
741.69059 3 Positive 44.3 50.3
750.3639 2 Positive 44.3 50.3
735.843 2 Positive 44.4 50.4
543.75513 2 Positive 44.4 50.4
778.36247 3 Positive 44.4 50.4
786.33891 2 Positive 44.4 50.4
642.80685 2 Positive 44.4 50.4
584.26739 3 Positive 44.5 50.5
636.29447 5 Positive 44.5 50.5
781.89089 2 Positive 44.5 50.5
728.33202000000006 3 Positive 44.6 50.6
462.26198 3 Positive 44.6 50.6
564.29841 3 Positive 44.6 50.6
845.94398 2 Positive 44.6 50.6
603.28943 3 Positive 44.6 50.6
807.88381 2 Positive 44.6 50.6
622.26915 4 Positive 44.6 50.6
534.92871 3 Positive 44.6 50.6
728.77867 2 Positive 44.6 50.6
649.54445 4 Positive 44.7 50.7
709.12075 5 Positive 44.7 50.7
546.7381 2 Positive 44.7 50.7
549.77332 2 Positive 44.7 50.7
589.96081 3 Positive 44.7 50.7
610.76094 2 Positive 44.8 50.8
792.68991 3 Positive 44.8 50.8
738.82035 2 Positive 44.8 50.8
509.57416 3 Positive 44.8 50.8
624.27237 3 Positive 44.8 50.8
656.28204 2 Positive 44.8 50.8
669.62877 3 Positive 44.9 50.9
462.00555 4 Positive 44.9 50.9
838.37014 4 Positive 45 51
687.70507 3 Positive 45 51
695.8 4 Positive 45 51
750.38136 3 Positive 45 51
732.85591 2 Positive 45 51
616.9612 3 Positive 45 51
590.28304 3 Positive 45.1 51.1
855.82656 2 Positive 45.1 51.1
530.24968 2 Positive 45.2 51.2
624.31313 4 Positive 45.2 51.2
718.32949 3 Positive 45.2 51.2
691.87589 2 Positive 45.2 51.2
707.0001 3 Positive 45.2 51.2
566.9509 3 Positive 45.2 51.2
657.31536 2 Positive 45.2 51.2
488.54444 3 Positive 45.2 51.2
601.3024 4 Positive 45.2 51.2
751.3256 2 Positive 45.2 51.2
523.23691 2 Positive 45.2 51.2
615.27369 2 Positive 45.2 51.2
832.08175 3 Positive 45.3 51.3
509.93219 3 Positive 45.3 51.3
519.22951 3 Positive 45.3 51.3
775.35235 2 Positive 45.3 51.3
576.01124 4 Positive 45.3 51.3
537.98612 3 Positive 45.4 51.4
604.60302 3 Positive 45.4 51.4
501.21949 3 Positive 45.4 51.4
575.24883 2 Positive 45.4 51.4
744.78247 2 Positive 45.5 51.5
554.29676 4 Positive 45.5 51.5
745.03217 3 Positive 45.5 51.5
863.41642 2 Positive 45.5 51.5
666.56422 4 Positive 45.5 51.5
537.25256 2 Positive 45.5 51.5
767.00127 3 Positive 45.5 51.5
628.69015 3 Positive 45.6 51.6
663.96193 3 Positive 45.6 51.6
579.29451 3 Positive 45.6 51.6
603.2773 3 Positive 45.6 51.6
874.07343 3 Positive 45.6 51.6
671.34834 3 Positive 45.6 51.6
753.70848 3 Positive 45.6 51.6
762.37735 3 Positive 45.6 51.6
738.72658 3 Positive 45.7 51.7
616.29468 4 Positive 45.7 51.7
469.90021 3 Positive 45.7 51.7
522.59267 3 Positive 45.7 51.7
582.66096 3 Positive 45.7 51.7
529.30043 3 Positive 45.7 51.7
901.71955 3 Positive 45.8 51.8
627.30986 5 Positive 45.8 51.8
783.38536 2 Positive 45.8 51.8
835.73967 3 Positive 45.8 51.8
652.28773 5 Positive 45.9 51.9
692.86552 2 Positive 45.9 51.9
618.31173 3 Positive 45.9 51.9
545.29535 3 Positive 45.9 51.9
496.57836 3 Positive 45.9 51.9
608.27337 3 Positive 45.9 51.9
456.5386 4 Positive 46 52
518.93307 3 Positive 46 52
601.64218 3 Positive 46 52
620.94928 3 Positive 46 52
551.61849 3 Positive 46.1 52.1
565.25707 3 Positive 46.1 52.1
694.81233 2 Positive 46.2 52.2
679.31354 2 Positive 46.2 52.2
452.91447 3 Positive 46.2 52.2
892.37895 3 Positive 46.2 52.2
455.23184 3 Positive 46.2 52.2
634.93363 3 Positive 46.2 52.2
666.5987 3 Positive 46.2 52.2
473.27869 4 Positive 46.3 52.3
482.58215 3 Positive 46.3 52.3
565.27095 4 Positive 46.3 52.3
753.35884 3 Positive 46.3 52.3
550.27472 3 Positive 46.3 52.3
682.94242 3 Positive 46.3 52.3
544.52357 4 Positive 46.4 52.4
697.35554 2 Positive 46.4 52.4
582.62994 3 Positive 46.4 52.4
849.92272 2 Positive 46.4 52.4
631.78442 2 Positive 46.4 52.4
658.96286 3 Positive 46.4 52.4
794.82224 2 Positive 46.4 52.4
603.30457 3 Positive 46.5 52.5
471.76943 4 Positive 46.5 52.5
886.14912 4 Positive 46.5 52.5
753.68176 3 Positive 46.5 52.5
746.85981 2 Positive 46.5 52.5
617.76877 2 Positive 46.6 52.6
770.01049 3 Positive 46.6 52.6
690.32245 3 Positive 46.6 52.6
780.96298 3 Positive 46.6 52.6
451.48346 4 Positive 46.6 52.6
821.87405 2 Positive 46.6 52.6
498.2423 3 Positive 46.6 52.6
504.23823 3 Positive 46.7 52.7
520.60127 3 Positive 46.7 52.7
619.81637 4 Positive 46.7 52.7
552.59842 3 Positive 46.7 52.7
658.26775 2 Positive 46.7 52.7
620.32296 2 Positive 46.7 52.7
720.69406 3 Positive 46.8 52.8
804.89363 2 Positive 46.8 52.8
814.6856 3 Positive 46.8 52.8
556.03837 4 Positive 46.8 52.8
650.33461 2 Positive 46.8 52.8
557.25563 2 Positive 46.8 52.8
597.98881 3 Positive 46.9 52.9
527.62358 3 Positive 46.9 52.9
856.01188 3 Positive 46.9 52.9
707.0001 3 Positive 47 53
628.29447 4 Positive 47 53
690.37044 2 Positive 47 53
518.77745 4 Positive 47.1 53.1
469.60222 3 Positive 47.1 53.1
570.2866 2 Positive 47.1 53.1
606.67282 3 Positive 47.1 53.1
549.77332 2 Positive 47.1 53.1
722.36326 3 Positive 47.2 53.2
837.3902 3 Positive 47.2 53.2
766.65055 3 Positive 47.2 53.2
601.77554 2 Positive 47.2 53.2
646.29149 3 Positive 47.2 53.2
555.95588 3 Positive 47.2 53.2
582.2661 3 Positive 47.3 53.3
530.21725 3 Positive 47.3 53.3
527.2338 3 Positive 47.3 53.3
594.31681 3 Positive 47.3 53.3
691.36751 3 Positive 47.4 53.4
578.28496 3 Positive 47.4 53.4
581.7764 2 Positive 47.4 53.4
597.27369 2 Positive 47.4 53.4
628.65913 3 Positive 47.5 53.5
675.34244 2 Positive 47.5 53.5
864.38578 3 Positive 47.5 53.5
547.92077 3 Positive 47.5 53.5
645.99881 3 Positive 47.5 53.5
744.84828 2 Positive 47.5 53.5
575.29753 3 Positive 47.6 53.6
636.28626 4 Positive 47.6 53.6
646.8317 2 Positive 47.6 53.6
664.8468 2 Positive 47.6 53.6
705.81507 2 Positive 47.7 53.7
572.93855 3 Positive 47.7 53.7
720.00401 3 Positive 47.7 53.7
774.38741 3 Positive 47.7 53.7
709.94761 3 Positive 47.7 53.7
518.89875 3 Positive 47.7 53.7
615.77131 2 Positive 47.7 53.7
591.96058 3 Positive 47.7 53.7
532.29546 3 Positive 47.8 53.8
744.33202000000006 3 Positive 47.8 53.8
557.32128 3 Positive 47.8 53.8
533.92408 3 Positive 47.9 53.9
558.04667 4 Positive 48 54
883.06393 3 Positive 48 54
612.29938 3 Positive 48.1 54.1
791.03408 3 Positive 48.1 54.1
568.94436 3 Positive 48.1 54.1
563.9745 3 Positive 48.1 54.1
679.6476 3 Positive 48.1 54.1
809.38672999999994 3 Positive 48.2 54.2
625.27199 3 Positive 48.2 54.2
699.03649 3 Positive 48.2 54.2
739.65123 3 Positive 48.3 54.3
609.78267 4 Positive 48.3 54.3
800.38249 2 Positive 48.3 54.3
703.34997 4 Positive 48.3 54.3
603.31569 2 Positive 48.3 54.3
513.61631 3 Positive 48.3 54.3
591.26895 4 Positive 48.3 54.3
511.5939 3 Positive 48.4 54.4
794.34048 2 Positive 48.4 54.4
730.70412 3 Positive 48.4 54.4
505.7653 2 Positive 48.4 54.4
766.88721 2 Positive 48.5 54.5
829.35528 2 Positive 48.5 54.5
709.71132 5 Positive 48.6 54.6
633.9931 3 Positive 48.6 54.6
648.28606 4 Positive 48.6 54.6
517.28831 3 Positive 48.6 54.6
641.32371 2 Positive 48.6 54.6
669.36516 2 Positive 48.6 54.6
495.92592 3 Positive 48.6 54.6
536.05382 4 Positive 48.7 54.7
749.84247 2 Positive 48.7 54.7
767.86829 4 Positive 48.7 54.7
738.03099 3 Positive 48.7 54.7
674.26879 3 Positive 48.8 54.8
642.98791 3 Positive 48.8 54.8
633.28412 3 Positive 48.8 54.8
681.83897 2 Positive 48.8 54.8
488.78789 4 Positive 48.8 54.8
587.30184 4 Positive 48.8 54.8
609.28539 3 Positive 48.9 54.9
879.91468 2 Positive 48.9 54.9
800.70908 3 Positive 48.9 54.9
643.61664 3 Positive 48.9 54.9
608.62838 3 Positive 49 55
713.84247 2 Positive 49 55
693.33306 4 Positive 49 55
576.02995 4 Positive 49 55
609.773 2 Positive 49 55
548.62347 3 Positive 49.1 55.1
770.06147 3 Positive 49.1 55.1
546.63073 3 Positive 49.1 55.1
613.29434 3 Positive 49.1 55.1
702.98925 3 Positive 49.1 55.1
785.34927 2 Positive 49.1 55.1
527.76022 2 Positive 49.2 55.2
577.79205 2 Positive 49.2 55.2
733.65554 3 Positive 49.2 55.2
733.30909 2 Positive 49.2 55.2
549.24811 3 Positive 49.2 55.2
663.83444 2 Positive 49.3 55.3
885.85984 2 Positive 49.3 55.3
634.89779 3 Positive 49.3 55.3
949.67756 4 Positive 49.3 55.3
764.74437 2 Positive 49.4 55.4
512.03189 4 Positive 49.4 55.4
577.79792 4 Positive 49.4 55.4
597.30367 4 Positive 49.4 55.4
825.67434 3 Positive 49.5 55.5
680.03235 3 Positive 49.5 55.5
491.7512 4 Positive 49.5 55.5
883.63396 4 Positive 49.5 55.5
707.10862 5 Positive 49.5 55.5
1110.46326 2 Positive 49.5 55.5
973.9298 4 Positive 49.6 55.6
704.63604 3 Positive 49.6 55.6
745.84634 4 Positive 49.6 55.6
705.84501 2 Positive 49.6 55.6
822.71367 3 Positive 49.6 55.6
734.79549 2 Positive 49.7 55.7
607.26946 3 Positive 49.7 55.7
647.69356 3 Positive 49.7 55.7
578.03826 4 Positive 49.7 55.7
716.97789 3 Positive 49.7 55.7
730.7034 3 Positive 49.7 55.7
859.3776 2 Positive 49.7 55.7
712.36331 3 Positive 49.8 55.8
792.40775 2 Positive 49.8 55.8
814.35453 2 Positive 49.8 55.8
831.393 3 Positive 49.8 55.8
767.70417 3 Positive 49.8 55.8
517.23866 3 Positive 49.8 55.8
603.7657 2 Positive 49.9 55.9
667.93118 3 Positive 49.9 55.9
522.04727 4 Positive 49.9 55.9
731.85905 2 Positive 49.9 55.9
563.63793 3 Positive 49.9 55.9
857.7404 3 Positive 50 56
508.55465 3 Positive 50 56
575.47244 5 Positive 50 56
572.29467 3 Positive 50 56
659.07822 4 Positive 50 56
782.39464 2 Positive 50.1 56.1
744.11279 4 Positive 50.1 56.1
749.12442 4 Positive 50.1 56.1
712.03531 3 Positive 50.1 56.1
519.60496 3 Positive 50.2 56.2
767.86033 2 Positive 50.2 56.2
813.11573 4 Positive 50.2 56.2
556.0454 4 Positive 50.2 56.2
623.79657 4 Positive 50.2 56.2
561.63414 3 Positive 50.2 56.2
784.00116 3 Positive 50.3 56.3
909.71938 3 Positive 50.3 56.3
543.76276 2 Positive 50.3 56.3
967.0778 3 Positive 50.3 56.3
726.8741 2 Positive 50.4 56.4
853.89382 2 Positive 50.5 56.5
639.80795 4 Positive 50.5 56.5
825.05269 3 Positive 50.5 56.5
646.32042 2 Positive 50.5 56.5
774.36898 4 Positive 50.6 56.6
559.47917 5 Positive 50.6 56.6
741.05811 3 Positive 50.6 56.6
808.61912 4 Positive 50.6 56.6
621.32911 4 Positive 50.6 56.6
617.77787 4 Positive 50.7 56.7
636.58853 3 Positive 50.7 56.7
822.89213 2 Positive 50.7 56.7
631.25846 3 Positive 50.7 56.7
897.15366 4 Positive 50.7 56.7
667.69858 5 Positive 50.8 56.8
655.77267 2 Positive 50.8 56.8
731.31407 2 Positive 50.8 56.8
765.83792 4 Positive 50.8 56.8
580.63855 3 Positive 50.8 56.8
592.75121 2 Positive 50.8 56.8
638.63163 3 Positive 50.8 56.8
703.02061 3 Positive 50.8 56.8
969.66914 4 Positive 50.9 56.9
649.30322 3 Positive 50.9 56.9
719.09461 4 Positive 50.9 56.9
852.3847 2 Positive 50.9 56.9
716.66029 3 Positive 50.9 56.9
834.37141 4 Positive 51 57
575.80801 4 Positive 51 57
699.29944 4 Positive 51 57
740.6446 3 Positive 51 57
774.87269 2 Positive 51 57
784.83144 2 Positive 51 57
489.76717 4 Positive 51 57
705.86882 2 Positive 51.1 57.1
512.04782 5 Positive 51.1 57.1
711.02229 3 Positive 51.1 57.1
723.83888 2 Positive 51.1 57.1
843.4236 2 Positive 51.1 57.1
692.97494 3 Positive 51.1 57.1
887.32983 3 Positive 51.2 57.2
575.59981 3 Positive 51.2 57.2
832.34289 2 Positive 51.2 57.2
964.73258 3 Positive 51.3 57.3
1007.6894 3 Positive 51.3 57.3
1052.46441 2 Positive 51.3 57.3
663.36516 2 Positive 51.3 57.3
645.27521 3 Positive 51.3 57.3
562.61816 3 Positive 51.3 57.3
665.80397 2 Positive 51.3 57.3
531.61279 3 Positive 51.3 57.3
716.82767 4 Positive 51.4 57.4
628.91944 3 Positive 51.4 57.4
665.30373 3 Positive 51.4 57.4
606.58702 4 Positive 51.4 57.4
595.98154 3 Positive 51.4 57.4
827.36453 3 Positive 51.4 57.4
786.108 4 Positive 51.4 57.4
787.85987 4 Positive 51.4 57.4
650.92781 3 Positive 51.4 57.4
753.87134 2 Positive 51.5 57.5
519.78095 2 Positive 51.5 57.5
486.23525 3 Positive 51.5 57.5
917.14525 4 Positive 51.5 57.5
696.02727 3 Positive 51.5 57.5
806.87037 2 Positive 51.6 57.6
753.82563 2 Positive 51.6 57.6
705.33481 2 Positive 51.6 57.6
1020.78147 3 Positive 51.6 57.6
613.32022 3 Positive 51.6 57.6
583.59881 3 Positive 51.6 57.6
874.89458 2 Positive 51.6 57.6
645.31105 3 Positive 51.6 57.6
578.63777 3 Positive 51.7 57.7
639.31536 3 Positive 51.8 57.8
689.36605 3 Positive 51.8 57.8
684.03308 3 Positive 51.8 57.8
630.48935 5 Positive 51.8 57.8
816.71072 3 Positive 51.9 57.9
701.9787 3 Positive 51.9 57.9
615.49425 5 Positive 51.9 57.9
796.05616 3 Positive 51.9 57.9
652.68713 3 Positive 51.9 57.9
590.96952 3 Positive 52 58
657.63095 3 Positive 52 58
893.37959 3 Positive 52.1 58.1
654.26246 2 Positive 52.1 58.1
687.33682 2 Positive 52.1 58.1
563.61849 3 Positive 52.2 58.2
719.71843 3 Positive 52.2 58.2
629.35512 4 Positive 52.2 58.2
531.29183 3 Positive 52.2 58.2
786.38371 3 Positive 52.2 58.2
543.2101 3 Positive 52.2 58.2
694.00283 3 Positive 52.2 58.2
665.32471 5 Positive 52.2 58.2
607.96131 3 Positive 52.3 58.3
529.97232 3 Positive 52.3 58.3
718.0257 3 Positive 52.3 58.3
610.96774 3 Positive 52.3 58.3
544.60832 3 Positive 52.4 58.4
715.62968 4 Positive 52.4 58.4
596.02153 4 Positive 52.4 58.4
620.77521 4 Positive 52.4 58.4
584.30955 3 Positive 52.4 58.4
566.61614 3 Positive 52.5 58.5
595.79959 4 Positive 52.5 58.5
922.72782 3 Positive 52.5 58.5
649.85971 2 Positive 52.5 58.5
812.35208 3 Positive 52.6 58.6
689.01503 3 Positive 52.6 58.6
789.82563 2 Positive 52.6 58.6
678.67042 3 Positive 52.6 58.6
497.23537 3 Positive 52.6 58.6
722.03079 3 Positive 52.6 58.6
936.93053 2 Positive 52.7 58.7
640.28769 3 Positive 52.7 58.7
572.9693 3 Positive 52.7 58.7
794.35962 3 Positive 52.7 58.7
737.38374 2 Positive 52.8 58.8
777.39359 2 Positive 52.8 58.8
1092.89028 2 Positive 52.8 58.8
779.83819 4 Positive 52.8 58.8
761.41686 3 Positive 52.8 58.8
631.08938 4 Positive 52.8 58.8
823.90908 2 Positive 52.9 58.9
854.38995 2 Positive 52.9 58.9
538.24934 3 Positive 53 59
887.10706 4 Positive 53 59
820.05482 3 Positive 53 59
477.26673 3 Positive 53 59
567.01867 4 Positive 53 59
707.67517 3 Positive 53.1 59.1
727.66315 3 Positive 53.1 59.1
712.85283 2 Positive 53.1 59.1
549.60848 3 Positive 53.1 59.1
807.85145 4 Positive 53.1 59.1
622.33861 2 Positive 53.1 59.1
858.12839 4 Positive 53.1 59.1
685.58173 3 Positive 53.1 59.1
929.19229 4 Positive 53.2 59.2
635.28382 3 Positive 53.2 59.2
673.02218 3 Positive 53.2 59.2
698.97063 3 Positive 53.2 59.2
648.00642 3 Positive 53.2 59.2
805.85945 2 Positive 53.2 59.2
679.34257 3 Positive 53.2 59.2
736.31615 3 Positive 53.3 59.3
839.87781 4 Positive 53.3 59.3
624.85248 4 Positive 53.3 59.3
468.24688 3 Positive 53.3 59.3
586.26992 3 Positive 53.4 59.4
616.29735 3 Positive 53.4 59.4
907.45169 3 Positive 53.4 59.4
668.35706 3 Positive 53.4 59.4
601.97393 3 Positive 53.4 59.4
828.6107 4 Positive 53.4 59.4
647.07348 4 Positive 53.5 59.5
512.28016 4 Positive 53.5 59.5
749.8454 4 Positive 53.5 59.5
664.33956 3 Positive 53.5 59.5
549.55646 4 Positive 53.5 59.5
479.00984 4 Positive 53.5 59.5
661.26707 3 Positive 53.5 59.5
550.31111 3 Positive 53.6 59.6
675.34696 2 Positive 53.6 59.6
815.36763 5 Positive 53.6 59.6
858.04844 3 Positive 53.6 59.6
696.68125 3 Positive 53.6 59.6
729.33839 3 Positive 53.6 59.6
746.86099 2 Positive 53.6 59.6
740.6759 3 Positive 53.7 59.7
688.33006 3 Positive 53.7 59.7
898.98147 5 Positive 53.7 59.7
758.36455 4 Positive 53.7 59.7
512.62855 3 Positive 53.7 59.7
796.01703 3 Positive 53.7 59.7
614.35488 3 Positive 53.8 59.8
960.3757 2 Positive 53.8 59.8
768.36018 4 Positive 53.8 59.8
850.02351 3 Positive 53.9 59.9
647.04968 3 Positive 53.9 59.9
681.04629 4 Positive 53.9 59.9
704.82996 2 Positive 53.9 59.9
532.78878 2 Positive 53.9 59.9
493.26539 3 Positive 54 60
790.74342 3 Positive 54 60
779.87572 4 Positive 54 60
602.53874 4 Positive 54 60
813.03594 3 Positive 54 60
625.6641 3 Positive 54 60
673.27505 2 Positive 54 60
568.62268 3 Positive 54 60
745.85812 2 Positive 54 60
715.9811 3 Positive 54.1 60.1
491.78789 4 Positive 54.1 60.1
760.66806 3 Positive 54.1 60.1
793.65671 3 Positive 54.1 60.1
696.10872 4 Positive 54.1 60.1
1024.14448 3 Positive 54.1 60.1
844.60868 4 Positive 54.2 60.2
772.00307 3 Positive 54.2 60.2
867.7722 3 Positive 54.2 60.2
559.31474 3 Positive 54.2 60.2
616.77914 2 Positive 54.3 60.3
716.64817 3 Positive 54.3 60.3
1028.06767 3 Positive 54.3 60.3
495.26891 3 Positive 54.4 60.4
608.64834 3 Positive 54.4 60.4
607.28943 3 Positive 54.4 60.4
678.67042 3 Positive 54.5 60.5
549.63923 3 Positive 54.5 60.5
595.00384 3 Positive 54.5 60.5
648.07715 4 Positive 54.5 60.5
586.32804 2 Positive 54.5 60.5
768.83241 2 Positive 54.5 60.5
657.98293 3 Positive 54.5 60.5
651.08097 4 Positive 54.5 60.5
631.3288 2 Positive 54.5 60.5
617.63408 3 Positive 54.6 60.6
803.74128 3 Positive 54.6 60.6
510.92201 3 Positive 54.6 60.6
521.06623 5 Positive 54.6 60.6
933.45521 4 Positive 54.7 60.7
610.57539 4 Positive 54.7 60.7
633.94487 3 Positive 54.7 60.7
671.96649 3 Positive 54.7 60.7
693.26611 3 Positive 54.8 60.8
610.35488 3 Positive 54.8 60.8
777.02984 3 Positive 54.8 60.8
554.28685 3 Positive 54.8 60.8
545.74847 2 Positive 54.8 60.8
622.02808 4 Positive 54.9 60.9
1007.4461 2 Positive 54.9 60.9
668.31144 3 Positive 54.9 60.9
628.02665 3 Positive 54.9 60.9
659.05323 4 Positive 54.9 60.9
603.05778 4 Positive 55 61
575.33693 3 Positive 55 61
572.28406 2 Positive 55 61
532.62347 3 Positive 55 61
773.35867 4 Positive 55 61
697.37374 2 Positive 55 61
631.32374 3 Positive 55 61
764.0305 3 Positive 55 61
570.30479 2 Positive 55.1 61.1
691.33907 4 Positive 55.1 61.1
1018.08786 3 Positive 55.1 61.1
820.08967 4 Positive 55.1 61.1
789.10758 4 Positive 55.1 61.1
844.91395 2 Positive 55.2 61.2
609.27433 3 Positive 55.2 61.2
603.55486 4 Positive 55.2 61.2
724.36812 3 Positive 55.2 61.2
929.64325 4 Positive 55.3 61.3
670.30475 3 Positive 55.3 61.3
797.66661 3 Positive 55.3 61.3
880.41485 3 Positive 55.3 61.3
676.31468 4 Positive 55.3 61.3
551.26101 3 Positive 55.3 61.3
503.92217 3 Positive 55.3 61.3
597.29188 2 Positive 55.4 61.4
737.30805 3 Positive 55.4 61.4
504.22669 4 Positive 55.4 61.4
592.28562 5 Positive 55.4 61.4
563.61173 3 Positive 55.5 61.5
780.6816 3 Positive 55.5 61.5
844.68977 3 Positive 55.5 61.5
928.9685 4 Positive 55.5 61.5
617.28552 4 Positive 55.5 61.5
543.52791 4 Positive 55.5 61.5
878.40188 3 Positive 55.5 61.5
562.28914 2 Positive 55.6 61.6
617.33134 2 Positive 55.6 61.6
737.0622 3 Positive 55.6 61.6
715.37455 3 Positive 55.6 61.6
456.7441 4 Positive 55.6 61.6
891.41926 3 Positive 55.6 61.6
822.71161 3 Positive 55.6 61.6
803.36271 2 Positive 55.7 61.7
635.96862 3 Positive 55.7 61.7
668.81626000000006 4 Positive 55.7 61.7
680.84058 4 Positive 55.7 61.7
744.68114 3 Positive 55.7 61.7
748.68623 3 Positive 55.7 61.7
769.59384 4 Positive 55.7 61.7
765.36214 3 Positive 55.7 61.7
734.00972 3 Positive 55.8 61.8
903.40077 3 Positive 55.8 61.8
791.01313 3 Positive 55.8 61.8
678.37044 2 Positive 55.8 61.8
763.15017 4 Positive 55.8 61.8
937.77136 3 Positive 55.9 61.9
715.67047 3 Positive 55.9 61.9
675.96741 3 Positive 55.9 61.9
737.00998 3 Positive 55.9 61.9
856.99939 2 Positive 55.9 61.9
705.97778 3 Positive 56 62
664.12095 4 Positive 56 62
593.5802 4 Positive 56 62
622.33861 2 Positive 56 62
824.39305 2 Positive 56 62
840.69087 3 Positive 56 62
740.1052 4 Positive 56 62
648.99143 3 Positive 56.1 62.1
639.31966 3 Positive 56.1 62.1
638.33464 3 Positive 56.1 62.1
835.38523 2 Positive 56.1 62.1
939.79305 3 Positive 56.2 62.2
692.37578 5 Positive 56.2 62.2
813.41865 2 Positive 56.2 62.2
533.74965 2 Positive 56.3 62.3
777.36691 2 Positive 56.3 62.3
644.8225 2 Positive 56.3 62.3
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