Skip to content

Instantly share code, notes, and snippets.

Embed
What would you like to do?
This file is an example of a simple bacterial incubation and OD600 experiment documented in the Autoprotocol JSON data standard.
{
"refs": {
"growth_plate": {
"id": "3208kfhf394",
"store": {
"where": "cold_4"
}
},
"DH5a": {
"discard": true,
"id": "3209xifd029"
}
},
"instructions": [
{
"reagent": "lb-broth-noAB",
"object": "growth_plate",
"columns": [
{
"column": "0",
"volume": "150:microliter"
}
],
"op": "dispense"
},
{
"groups": [
{
"transfer": [
{
"volume": "2.0:microliter",
"to": "growth_plate/0",
"from": "DH5a/0"
}
]
},
{
"transfer": [
{
"volume": "2.0:microliter",
"to": "growth_plate/12",
"from": "DH5a/0"
}
]
},
{
"transfer": [
{
"volume": "2.0:microliter",
"to": "growth_plate/24",
"from": "DH5a/0"
}
]
},
{
"transfer": [
{
"volume": "2.0:microliter",
"to": "growth_plate/36",
"from": "DH5a/0"
}
]
}
],
"op": "pipette"
},
{
"lid": "low_evaporation",
"object": "growth_plate",
"op": "cover"
},
{
"where": "warm_37",
"object": "growth_plate",
"co2_percent": 0,
"duration": "12:hour",
"shaking": true,
"op": "incubate"
},
{
"dataref": "OD600",
"object": "growth_plate",
"wells": ["A1", "B1", "C1", "D1", "E1", "F1", "G1", "H1"],
"num_flashes": 25,
"wavelength": "600:nanometer",
"op": "absorbance"
}
]
}
@bmiles

This comment has been minimized.

Copy link
Owner Author

@bmiles bmiles commented Sep 24, 2015

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
You can’t perform that action at this time.