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Download rsids for annotation
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#!/usr/bin/bash | |
# @author = Daniel E. Cook | |
# @date = 2017-10-09 | |
# This script downloads rs identifiers | |
# The function 'annotate_rsid' can be used to annotate a strat file with rsid. | |
# Usage: | |
# zcat chr1.strat.frq.gz | annotate_rside - | bgzip > out.bed.gz | |
curl -s http://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/snp150.txt.gz | \ | |
gunzip -c | \ | |
awk -v OFS='\t' '{ print $2, $3, $4, $5, $10 }' | \ | |
sed 's/chr//g' | \ | |
bgzip -c > snps150.txt.bed.gz | |
tabix -p snps150.txt.bed.gz | |
function annotate_rsid() { | |
awk -F $'\t' ''' | |
{ | |
chrom_pos = $1 ":" $2 "-" $2; | |
command = "tabix /var/www/genetic_reference/snps150.txt.bed.gz " chrom_pos | |
if (command != command_last) { | |
command | getline result; | |
} | |
close(command); | |
split(result, rs, "\t"); | |
chrom = rs[1]; | |
pos = rs[3]; | |
rs_id = rs[4]; | |
ref_alt = $5 "/" $6; | |
alt_ref = $6 "/" $5; | |
test_ref_alt = rs[5]; | |
if ($1 == chrom && $2 == pos) { | |
out = 1; | |
if (ref_alt == test_ref_alt) { | |
ref = $5; | |
alt = $6; | |
out = 0; | |
} else if (alt_ref == test_ref_alt) { | |
ref = $6; | |
alt = $5; | |
out = 0; | |
} | |
if (out == 0) { | |
print $1 "\t" $2 "\t" rs_id "\t" $4 "\t" ref "\t" alt "\t" $7 "\t" $8 "\t" $9 "\t" $10; | |
} else { | |
print $1 "\t" $2 "\t\t" $4 "\t" $5 "\t" $6 "\t" $7 "\t" $8 "\t" $9 "\t" $10; | |
} | |
} | |
command_last = command; | |
} | |
''' $1 | |
} |
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