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%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% Reads DICOM-Files exported with
% p:\doc\MeVisLab-Networks\2011\ExtractAcinusAndExport.mlab and save them
% as JPG image sequences to further peruse with the STEPanizer.com
% First version: 20.06.2011, reading and displaying the DICOM-file
% 28.07.2011: Now reads ALL the DICOM-Files in the directory selected on
% Line 16 and converts them in one batch.
clc
clear all
close all
%% 04A - 2010a - finished - they're there
%% 04B - 2010a - finished - they're there
%% 04C - 2010a - finished - they're there
%% 04D - 2010c - finished - they're there
%% 04E - 2010c - finished - they're there
%% 10A - 2010a - finished - they're there
%% 10B - 2010a - finished - they're there
%% 10C - 2010c - finished - they're there
%% 10D - 2010c - finished - they're there
%% 10E - 2010c - finished - they're there
%% 21A - 2009f - finished - they're there
%% 21B - 2010a - finished - they're there
%% 21C
%% 21D - 2010c - finished - they're there
%% 21E - 2010c - finished - they're there
%% 36A - 2010b - finished - they're there
%% 36B - 2009b - finished - they're there
%% 36C - 2010a - finished - they're there
%% 36D - 2010a - finished - they're there
%% 36E - 2009f - segmentation not possible
%% 60A
%% 60B - 2010c - finished
%% 60C
%% 60D - 2009f - finished
%% 60E - 2009f - finished
Scalebar = 100; % micrometer
SliceDistance = 10;
tif = 0; % read only tiff-Files (if tif=0, read DICOM)
SamplePath = uigetdir('R:\','Select *Directory* of the Sample you want to convert the DICOM-Files from MeVisLab to JPG for STEPanizer');
for Disector = [0,1];
for DisectorThickness = [3,5,7];
if DisectorThickness>=SliceDistance
disp(['DisectorThickness (' num2str(DisectorThickness) ') is equal or larger than Slicedistance (' num2str(SliceDistance) '), please redefine in MATLAB-File'])
break
end
disp([ 'Counting DICOM-Files in ' SamplePath ]);
if tif == 1
disp('Reading TIFF files')
filelist = dir([SamplePath filesep '*.tif']);
else
disp('Reading DICOM files')
filelist = dir([SamplePath filesep '*.dcm']);
end
NumberOfDICOMFiles = size(filelist,1);
disp([ 'Found ' num2str(NumberOfDICOMFiles) ' DICOMs in ' SamplePath]);
%% Iterate through all the files found in the directory and save them do
%% JPG-slices
for i=1:NumberOfDICOMFiles
disp([ 'Saving ' filelist(i).name ' to JPG-slices']);
PathToDICOMFile = [SamplePath filesep]; % Carried over from first manual script, just leave it for the moment...
DICOMFileName = filelist(i).name; % Carried over from first manual script
%% Extract SampleName and Number of Acinus and make directory to save slices into
SampleNameStartPointer = regexp(PathToDICOMFile, 'R108', 'once');
SampleName = PathToDICOMFile(SampleNameStartPointer:end-1);
VoxelSizeStartPointer = regexp(DICOMFileName, 'pixelsize', 'once');
VoxelSize = 1000*str2num(DICOMFileName(VoxelSizeStartPointer+9:end-4)); % VoxelSize in micrometer
ScaleBarLength = round(Scalebar/VoxelSize);
VolumeStartPointer = regexp(DICOMFileName, 'volume', 'once');
VolumeEndPointer = regexp(DICOMFileName, 'pixelsize', 'once');
Volume = DICOMFileName(VolumeStartPointer+6:VolumeEndPointer-2);
AcinusStartPointer = regexp(DICOMFileName, 'acinus', 'once');
AcinusEndPointer = regexp(DICOMFileName, '.volume', 'once');
AcinusNumber = DICOMFileName(AcinusStartPointer+6:AcinusEndPointer-1); % remove "acinus", so we can format the number nicely
AcinusNumber = (sprintf('%02d',str2num(AcinusNumber))); % format string to number and pad with zero if necessary
AcinusName = [ 'acinus' AcinusNumber ];
if Disector == 0
AcinusPath = [PathToDICOMFile AcinusName filesep 'voxelsize' ...
num2str(VoxelSize) '-every' num2str(SliceDistance) 'slice' ];
elseif Disector == 1
AcinusPath = [PathToDICOMFile AcinusName filesep 'voxelsize' num2str(VoxelSize) ...
'-every' num2str(SliceDistance) 'slice-DisectorThickness-' num2str(sprintf('%1.2f',DisectorThickness * VoxelSize)) ...
'um-or' num2str(DisectorThickness) 'slices' ];
else
warndlg('Please set Disector to either 0 or 1');
break
end
[status,message,messageid] = mkdir(AcinusPath);
%% actually read File
disp([ 'Reading File ' num2str(i) '/' num2str(NumberOfDICOMFiles) ]);
if tif == 1
DICOMFile = double(loadtiff([PathToDICOMFile DICOMFileName]));
else
DICOMFile = double(dicomread([PathToDICOMFile DICOMFileName])); % read in DICOM File as double
end
DICOMFile = DICOMFile / max(max(max(DICOMFile))).*255; % scale to a maximum value of 255, still in double
%% Show slices of the DICOM-File
slices = size(DICOMFile,4);
% subplotrows = 4;
% figure
% for ctr=1:(subplotrows^2)
% subplot(subplotrows,subplotrows,ctr)
% showslice = round(slices/(subplotrows^2)*ctr);
% imshow(DICOMFile(:,:,showslice),[]);
% title(['Slice ' num2str(showslice)])
% end
% pause(0.001)
disp('---');
%% Write out Slices to JPG images
% figure
SliceCounter = 0;
for slice = 1:SliceDistance:slices
disp(['writing file ' num2str(slice) '/' num2str(slices)])
SliceCounter = SliceCounter + 1;
% Pad CurrentSlice to square size of longer side
CurrentSlice = ones(max(size(DICOMFile(:,:,slice)))).*255; % Make square image with larger length of original DICOM file (white square)
if Disector == 0
CurrentSlice(1:size(DICOMFile(:,:,slice),1),1:size(DICOMFile(:,:,slice),2)) = DICOMFile(:,:,slice); % Write slice of DICOM file to top left corner of white square of above line
CurrentSlice = uint8(CurrentSlice); % convert to uint8 before saving and displaying
% Make Scalebar
CurrentSlice(size(DICOMFile,1)-10-(round(ScaleBarLength/10)):size(DICOMFile,1)-10,10:10+ScaleBarLength) = 255; % draw Scalebar of length(ScaleBarLength) in the bottom left corner, with 10 times the length of the height.
WriteFileName = [ SampleName '-' AcinusName '_' num2str(SliceCounter) '.jpg' ];
imwrite(CurrentSlice,[AcinusPath filesep WriteFileName]);
else
if slice+DisectorThickness<=size(DICOMFile,4) % only try to write slice if we actually can and if the next slice (for disector) is not out of bounds.
% write slice as "_a.jpg"
CurrentSlice(1:size(DICOMFile(:,:,slice),1),1:size(DICOMFile(:,:,slice),2)) = DICOMFile(:,:,slice); % Write slice of DICOM file to top left corner of white square of above line
CurrentSlice = uint8(CurrentSlice); % convert to uint8 before saving and displaying
% Make Scalebar
CurrentSlice(size(DICOMFile,1)-10-(round(ScaleBarLength/10)):size(DICOMFile,1)-10,10:10+ScaleBarLength) = 255; % draw Scalebar of length(ScaleBarLength) in the bottom left corner, with 10 times the length of the height.
WriteFileName = [ SampleName '-' AcinusName '_' num2str(SliceCounter) '_a.jpg' ];
imwrite(CurrentSlice,[AcinusPath filesep WriteFileName]);
% write successive slice as "_b.jpg"
CurrentSlice(1:size(DICOMFile(:,:,slice),1),1:size(DICOMFile(:,:,slice),2)) = DICOMFile(:,:,slice+DisectorThickness); % Write 'slice+DisectorThickness' of DICOM file to top left corner of white square of above line
CurrentSlice = uint8(CurrentSlice); % convert to uint8 before saving and displaying
% Make Scalebar
CurrentSlice(size(DICOMFile,1)-10-(round(ScaleBarLength/10)):size(DICOMFile,1)-10,10:10+ScaleBarLength) = 255; % draw Scalebar of length(ScaleBarLength) in the bottom left corner, with 10 times the length of the height.
WriteFileName = [ SampleName '-' AcinusName '_' num2str(SliceCounter) '_b.jpg' ];
imwrite(CurrentSlice,[AcinusPath filesep WriteFileName]);
end
end
end
%% Give out some info
disp(['I have written ' AcinusName ' with Volume ' num2str(Volume) ' to ' AcinusPath filesep SampleName '-' AcinusName '-x.jpg']);
disp(['I have witten every ' num2str(SliceDistance) 'th slice!'])
disp(['The scalebar on the image is ' num2str(Scalebar) ' micrometer long.'])
clear DICOMFile
disp('---')
pause(0.001)
close all
end
end
end
disp('Finished!')
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