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# Download Kallisto and sratools (the latter to be able to download from SRA) | |
wget https://github.com/pachterlab/kallisto/releases/download/v0.42.3/kallisto_mac-v0.42.3.tar.gz | |
tar zvxf kallisto_mac-v0.42.3.tar.gz | |
wget http://ftp-trace.ncbi.nlm.nih.gov/sra/sdk/2.5.2/sratoolkit.2.5.2-mac64.tar.gz | |
tar zxvf sratoolkit.2.5.2-mac64.tar.gz | |
# Download and merge human cDNA and ncDNA files from Ensembl for the index. | |
wget ftp://ftp.ensembl.org/pub/current_fasta/homo_sapiens/cdna/Homo_sapiens.GRCh38.cdna.all.fa.gz | |
wget ftp://ftp.ensembl.org/pub/current_fasta/homo_sapiens/ncrna/Homo_sapiens.GRCh38.ncrna.fa.gz | |
cat Homo_sapiens.GRCh38.cdna.all.fa.gz Homo_sapiens.GRCh38.ncrna.fa.gz > Homo_sapiens.GRCh38.rna.fa.gz |
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import tensorflow as tf | |
import numpy as np | |
import math | |
#import pandas as pd | |
#import sys | |
input = np.array([[2.0, 1.0, 1.0, 2.0], | |
[-2.0, 1.0, -1.0, 2.0], | |
[0.0, 1.0, 0.0, 2.0], | |
[0.0, -1.0, 0.0, -2.0], |
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""" | |
* Converts images to GGB (grayscale) | |
* Creates subsets for training and validation | |
* Adds columns to indicate training or validation (useful for analysis of the deployed model) | |
* Adds rotated images to the dataset | |
* Creates comma-separated CSV file | |
* Creates zip archive | |
""" | |
import pandas as pd |
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1. Install appropriate version of the Tensorflow (Python) framework from https://www.tensorflow.org/versions/r0.12/get_started/os_setup.html | |
In my case (Mac OS X 10.11), I did: | |
- Get the .whl file (this is more likely to work than a direct pip install) | |
wget https://storage.googleapis.com/tensorflow/mac/cpu/tensorflow-0.11.0-py3-none-any.whl | |
- Install using non-Anaconda pip | |
/usr/local/bin/pip3 install tensorflow-0.11.0-py3-none-any.whl_ |
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from pathlib import Path | |
import os | |
import sys | |
from fire import Fire | |
import numpy as np | |
import pandas as pd | |
from tqdm import tqdm | |
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def read_single_cossmo_example(serialized_example, n_tissues=1, coord_sys='rna1'): | |
"""Decode a single COSSMO example | |
coord_sys must be one of 'rna1' or 'dna0', if 'dna0' then an extra 'strand' field | |
must exist in the tfrecord and is extracted. | |
""" | |
assert coord_sys in ['dna0', 'rna1'] | |
context_features = { |
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# Installation (only needs to be done once) | |
source("http://bioconductor.org/biocLite.R") | |
biocLite("rhdf5") | |
install.packages("devtools") | |
devtools::install_github("pachterlab/sleuth") | |
# Now load the package | |
library("sleuth") | |
# A function (borrowed from the Sleuth documentation) for connecting Ensembl transcript names to common gene names |
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library(tensorflow) | |
tf$reset_default_graph() | |
x_data <- runif(100, min=0, max=1) | |
y_data <- x_data * 0.1 + 0.3 + rnorm(n, mean=0, sd=0.025) | |
W <- tf$Variable(tf$random_uniform(shape(1L), -1.0, 1.0)) | |
b <- tf$Variable(tf$zeros(shape(1L))) | |
y <- W * x_data + b |
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import sys | |
import gzip | |
if len(sys.argv)<3: | |
sys.exit("python sum_per_gene.py <cDNA FASTA file> <TPM table>") | |
ensg = {} | |
mapf = gzip.open(sys.argv[1]) | |
ctr = 0 |
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args = commandArgs(trailingOnly=TRUE) | |
path=args[1] | |
files=Sys.glob(paste0(path,"/*/abundance.tsv")) | |
#print(files) | |
merge_two <- function(x,y){ | |
#print(dim(x)) | |
if ("tpm" %in% colnames(x)){ | |
x_ <- x[,c(1,5)] | |
} | |
else{ |
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