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@keller-mark
Last active June 2, 2023 20:37
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A description.md example for hubmap publication page vignette
version affiliation first_name last_name middle_name_or_initial name orcid_id is_contact
1 Harvard Medical School Mark Keller S Mark S Keller 0000-0003-3003-874X FALSE
Harvard Medical School Ilan Gold Ilan Gold 0000-0002-5823-1026 FALSE
Harvard Medical School Chuck McCallum Chuck McCallum 0000-0003-4039-9768 FALSE
Harvard Medical School Trevor Manz Trevor Manz 0000-0001-7694-5164 FALSE
Harvard Medical School Peter Kharchenko V Peter V Kharchenko 0000-0002-6036-5875 FALSE
Harvard Medical School Nils Gehlenborg Nils Gehlenborg 0000-0003-0327-8297 TRUE
name Use Case 1
figures
name file
Visualization
vitessce.json

Single-molecule fluorescence in situ hybridization from the mouse somatosensory cortex

Codeluppi et al. used single-molecule fluorescence in situ hybridization (smFISH) to profile the somatosensory cortex in a mouse brain section (Nature Methods 2018). The authors selected 33 marker genes based on previous scRNA-seq findings in the somatosensory cortex and their ability to define cell types. A notable finding from this experiment was the discovery of a transition region defined by the Pyramidal L3/4 excitatory neuron cell type. Using the spatial plot and heatmap in Vitessce, we can reproduce this finding and observe the reported joint expression of markers Lamp5 and Rorb that define the surrounding Pyramidal L2/3 and L4 cell types, respectively.

version assay_type contributors_path data_path
0 Publication extras/contributors.tsv .
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