DEXSeq prepare annotation throws error for RNACentral GTF file
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# Convert GFF3 to GTF file | |
wget ftp://ftp.ebi.ac.uk/pub/databases/RNAcentral/releases/5.0/genome_coordinates/Mus_musculus.GRCm38.gff3.gz | |
gunzip --verbose Mus_musculus.GRCm38.gff3.gz | |
# Download gffread source and compile it | |
mkdir gffread_build | |
cd gffread_build | |
git clone https://github.com/gpertea/gclib | |
git clone https://github.com/gpertea/gffread | |
cd gffread | |
make | |
# convert GFF3 to GTF | |
gffread ../../Mus_musculus.GRCm38.gff3 -T -o ../../Mus_musculus.GRCm38.gtf | |
# Remove gffread_build again | |
cd ../../ | |
rm -r -f gffread_build/ | |
# Download dexseq_count.py from the bioconductor GIT mirror | |
wget https://raw.githubusercontent.com/Bioconductor-mirror/DEXSeq/master/inst/python_scripts/dexseq_prepare_annotation.py | |
python dexseq_prepare_annotation.py Mus_musculus.GRCm38.gtf dexseq_prepare_annotation.out | |
# Yields the following error: | |
# Traceback (most recent call last): | |
# File "dexseq_prepare_annotation.py", line 54, in <module> | |
# f.attr['gene_id'] = f.attr['gene_id'].replace( ":", "_" ) | |
# KeyError: 'gene_id' |
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