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PT J | |
AU Lin, TH | |
Huang, CF | |
Guo, GL | |
Hwang, WS | |
Huang, SL | |
AF Lin, Ting-Hsiang | |
Huang, Chiung-Fang | |
Guo, Gia-Luen | |
Hwang, Wen-Song | |
Huang, Shir-Ly | |
TI Pilot-scale ethanol production from rice straw hydrolysates using | |
xylose-fermenting Pichia stipitis | |
SO BIORESOURCE TECHNOLOGY | |
LA English | |
DT Article | |
DE Ammonia solution neutralization; Cellulosic ethanol; Detoxification; | |
Ethanol production; Xylose fermentation | |
ID DILUTE-ACID PRETREATMENT; SACCHAROMYCES-CEREVISIAE; LIGNOCELLULOSIC | |
BIOMASS; CELLULOSIC ETHANOL; CANDIDA-SHEHATAE; FERMENTATION; GLUCOSE; | |
TOXICITY; YEAST; TECHNOLOGIES | |
AB Ethanol was produced at pilot scale from rice straw hydrolysates using a Pichia stipitis strain previously adapted to NaOH-neutralized hydrolysates. The highest ethanol yield was 0.44 +/- 0.02 g(p)/g(s) at an aeration rate of 0.05 vvm using overliming-detoxified hydrolysates. The yield with hydrolysates conditioned by ammonia and NaOH was 0.39 +/- 0.01 and 0.34 +/- 0.01 g(p)/g(s), respectively, were achieved at the same aeration rate. The actual ethanol yield from hydrolysate fermentation with ammonia neutralization was similar to that with overliming hydrolysate after taking into account the xylose loss resulting from these conditioning processes. Moreover, the ethanol yield from ammonia-neutralized hydrolysates could be further enhanced by increasing the initial cell density by two-fold or reducing the combined concentration of furfural and 5-hydroxymethyl furfural to 0.6 g/L by reducing the severity of operational conditions in pretreatment. This study demonstrated the potential for commercial ethanol production from rice straw via xylose fermentation. (C) 2012 Elsevier Ltd. All rights reserved. | |
C1 [Lin, Ting-Hsiang; Huang, Shir-Ly] Natl Cent Univ, Dept Life Sci, Jhongli 32001, Taoyuan County, Taiwan. | |
[Huang, Chiung-Fang; Guo, Gia-Luen; Hwang, Wen-Song] Inst Nucl Energy Res, Div Chem, Longtan Township 32546, Taoyuan County, Taiwan. | |
RP Huang, SL (reprint author), Natl Cent Univ, Dept Life Sci, 300 Jhongda Rd, Jhongli 32001, Taoyuan County, Taiwan. | |
EM slhuang@cc.ncu.edu.tw | |
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NR 30 | |
TC 6 | |
Z9 6 | |
PU ELSEVIER SCI LTD | |
PI OXFORD | |
PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND | |
SN 0960-8524 | |
J9 BIORESOURCE TECHNOL | |
JI Bioresour. Technol. | |
PD JUL | |
PY 2012 | |
VL 116 | |
BP 314 | |
EP 319 | |
DI 10.1016/j.biortech.2012.03.089 | |
PG 6 | |
WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & | |
Fuels | |
SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | |
GA 963BP | |
UT WOS:000305595400048 | |
ER | |
PT J | |
AU Brodeur-Campbell, M | |
Klinger, J | |
Shonnard, D | |
AF Brodeur-Campbell, Michael | |
Klinger, Jordan | |
Shonnard, David | |
TI Feedstock mixture effects on sugar monomer recovery following dilute | |
acid pretreatment and enzymatic hydrolysis | |
SO BIORESOURCE TECHNOLOGY | |
LA English | |
DT Article | |
DE Lignocellulosic biomass; Ethanol; Pretreatment; Enzymatic hydrolysis; | |
Optimization | |
ID KINETIC CHARACTERIZATION; ETHANOL-PRODUCTION; SWITCHGRASS; CELLULOSE; | |
SOFTWOOD; SACCHARIFICATION; YIELDS | |
AB This study seeks to investigate the effects of biomass mixtures on overall sugar recovery from the combined processes of dilute acid pretreatment and enzymatic hydrolysis. Aspen, a hardwood species well suited to biochemical processing, was chosen as the model species for this study. Balsam, a high-lignin softwood species, and switchgrass, an herbaceous energy crop with high ash content, were chosen as adjuncts. A matrix of three different dilute acid pretreatment severities and three different enzyme loading levels was used to characterize interactions between pretreatment and enzymatic hydrolysis. No synergism or antagonism was observed for any of the feedstock mixtures. Maximum glucose yield was 70% of theoretical for switchgrass and maximum xylose yield was 99.7% of theoretical for aspen. Supplemental p-glucosidase increased glucose yield from enzymatic hydrolysis by an average of 15%. Total sugar recoveries for mixtures could be predicted to within 4% by linear interpolation of the pure species results. (C) 2012 Elsevier Ltd. All rights reserved. | |
C1 [Brodeur-Campbell, Michael; Klinger, Jordan; Shonnard, David] Michigan Technol Univ, Dept Chem Engn, Houghton, MI 49931 USA. | |
[Shonnard, David] Michigan Technol Univ, Sustainable Futures Inst, Houghton, MI 49931 USA. | |
RP Brodeur-Campbell, M (reprint author), Michigan Technol Univ, Dept Chem Engn, 1400 Townsend Dr, Houghton, MI 49931 USA. | |
EM mcampbel@mtu.edu | |
FU National Science Foundation through Material Use: Science, Engineering | |
and Society (MUSES) award [BES-0524872]; National Science Foundation | |
through Integrative Graduate Education and Research Traineeships (IGERT) | |
award [DGE-0333401] | |
FX The authors would like to thank the National Science Foundation for | |
support through a Material Use: Science, Engineering and Society (MUSES) | |
award (BES-0524872) and through the Integrative Graduate Education and | |
Research Traineeships (IGERT) award (DGE-0333401). | |
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NR 17 | |
TC 3 | |
Z9 3 | |
PU ELSEVIER SCI LTD | |
PI OXFORD | |
PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND | |
SN 0960-8524 | |
J9 BIORESOURCE TECHNOL | |
JI Bioresour. Technol. | |
PD JUL | |
PY 2012 | |
VL 116 | |
BP 320 | |
EP 326 | |
DI 10.1016/j.biortech.2012.03.090 | |
PG 7 | |
WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & | |
Fuels | |
SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels | |
GA 963BP | |
UT WOS:000305595400049 | |
ER |
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