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sdwfrost/SRR765688.fasta

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Test files for vdjtools/ChangeO
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SRR765688.fasta
>SRR765688.30659 HQP9GKT01B1E02 length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTT
CTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCA
CAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCA
GCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAG
CACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTCGGCCTCCAC
>SRR765688.38189 HQP9GKT01B1F8D length=357|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTG
GCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCT
ACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACC
CAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGG
CTGTATATTATTGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGGGGCCAGGG
AACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.23676 HQP9GKT01CBCVC length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTT
CTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCA
CATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAG
GCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAG
CACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.44566 HQP9GKT01BUA3B length=393|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGC
CCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTT
TGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGA
GTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACC
GCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAAT
TATGGTGGCTTTGACTACTGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
>SRR765688.49541 HQP9GKT01ESY9C length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCT
CTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCA
CAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACA
ACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGA
TTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAGCCTCCAC
>SRR765688.45233 HQP9GKT01BVKNW length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAG
TGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATA
TATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTC
CGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACAC
GGCCGTGTATTACTGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGGGG
CCAGGGAACCCTGGTCACCGTCTCCTCGGCCTCCAC
>SRR765688.17591 HQP9GKT01CLJHN length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNCCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGC
TCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTG
GAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGT
CGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTG
ACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGATGGTTGTACTAAGGGTTTATGC
AGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC
>SRR765688.43976 HQP9GKT01CAKGO length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=14
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAG
CAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGAT
TGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCAT
GTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGA
CACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGC
TCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.49474 HQP9GKT01DTUWB length=384|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAG
CAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGA
TTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCA
TGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAG
ACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGG
CTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.34997 HQP9GKT01BF6LJ length=395|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTT
CTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACA
CAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATA
ACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAGCTTCCTGTAGCATTAGCA
GCTGCCATGCCCAGCAGTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCAA
CCTCCAC
>SRR765688.33811 HQP9GKT01AZ9Y4 length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTT
CTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGC
TTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCA
CAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCG
GTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATA
CCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCG
TCTCCTCAGCCTCCAC
>SRR765688.40396 HQP9GKT01DTRVH length=361|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGA
CTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGG
CCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCA
AGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACT
GTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCC
AGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.38176 HQP9GKT01CL3CG length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGC
CCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTT
TATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTC
AGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACAC
GGCCGTCTACTTCTGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTT
TGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAGCATCCCCG
>SRR765688.36681 HQP9GKT01B98GC length=382|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=13
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTT
CTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTC
TTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCA
CAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTG
GCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGAAGAACGTCGAAGTAGTG
GTTATTTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.26204 HQP9GKT01AGSP2 length=365|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAG
CTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATTATA
CAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGC
AGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGC
TGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGG
CCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.31319 HQP9GKT01B1IO7 length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTT
CTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCA
CAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATA
ACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGG
ACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.20699 HQP9GKT01DRRB0 length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTC
CTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCC
AGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGC
AGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTT
ACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGATGGACC
CAACTCCCCTCGACTACTGGGGCCTGGGAACCCTGGTCACCGTCGCTTCAGCCTCCAC
>SRR765688.48796 HQP9GKT01B94FW length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCA
GGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCA
GACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTG
CAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGAT
ACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGG
ACCACGGTCACCGTGTCCTCAGCCTCCAC
>SRR765688.41848 HQP9GKT01C3DX5 length=387|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCT
CTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCA
CAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCA
GCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGG
TGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCCGCAGCATCCC
CG
>SRR765688.23863 HQP9GKT01BPQLA length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTG
CTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACT
ACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGC
CAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGG
CTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGG
GAACCCTGGTCACCGTCTCCGCAACCTCCAC
>SRR765688.17127 HQP9GKT01CQJSD length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTT
CTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTA
GCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTT
TTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCCCG
>SRR765688.32432 HQP9GKT01CZ7DE length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATG
CTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCC
CTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGC
ACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCG
TTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGG
GCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
>SRR765688.20304 HQP9GKT01AMKZ5 length=386|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTT
CTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGAC
TTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCA
CAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTA
ACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGAGGGACTGTGGGACGGCA
GGGTTGTGACTGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATCCCCG
>SRR765688.47093 HQP9GKT01BTC88 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTT
CCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCA
CAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACA
ACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAA
CGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.46353 HQP9GKT01CZULB length=366|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAG
CTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTAT
ACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACG
CAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGG
CTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGG
GCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.49658 HQP9GKT01D85J4 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGC
CCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTT
TGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGA
GTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACC
GCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAAT
TATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
>SRR765688.31392 HQP9GKT01BBVPS length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=7
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCT
CTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCA
GCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.11728 HQP9GKT01AL5DZ length=389|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTG
GATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGG
AATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACG
GCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCC
TGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATA
CTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCA
C
>SRR765688.21411 HQP9GKT01CV52A length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNNGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTAC
TATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC
CCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGG
GACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCC
GTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGG
ACCACGGTCACCGCCTCCTCAGCATCCCCG
>SRR765688.46885 HQP9GKT01B8Z8I length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=7
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTT
CTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCA
CATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCG
GCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAG
CACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.15716 HQP9GKT01BPKZR length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTT
CTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGT
TTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACG
GCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGAATGGACTACACTCATTACC
ATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.28724 HQP9GKT01CM7HO length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTG
GAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTG
AGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGG
GTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCC
TGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACT
ACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
>SRR765688.35930 HQP9GKT01CJLTL length=406|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTG
GATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTG
AGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGG
GCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCC
TGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGAATATCGGAGAAGATTATAATTA
AACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGGGGCCAAGGGACCACGGTCAC
CGTCTCCTCAGCCTCCAC
>SRR765688.30106 HQP9GKT01CBSGA length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTT
CTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCA
CAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTA
ATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGACTTGACGGCAGCGATAGGG
ACTTTTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.42360 HQP9GKT01B8L6A length=364|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACT
GGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTC
ATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACT
AAGGACACCTCCAAGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACA
TATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGC
CAGGGAGCCCTGGTCACCGTGTCCTCAGCATCCCCG
>SRR765688.38856 HQP9GKT01A7EJO length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTA
CTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTA
CAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCC
AGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGC
ACTTTATTACTGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGGGGCCA
GGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.23240 HQP9GKT01BYYSD length=378|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATA
CATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTG
GATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACG
GTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAA
GGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAA
GGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.45110 HQP9GKT01BTIR2 length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTG
GTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCT
ACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACC
CAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGG
CTGTGTATTACTGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGGGGCCAAG
GGACCACGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.41920 HQP9GKT01DGU7R length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTT
CTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCA
CAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCA
GCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGC
CGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.5099 HQP9GKT01AS88P length=374|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTT
CTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCA
CAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCA
GTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGG
ACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.41370 HQP9GKT01DK3O2 length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTA
TTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCG
CAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCA
ACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACT
TCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.46467 HQP9GKT01EZF79 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTG
GATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTG
AGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGG
GCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCC
TGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTA
TGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG
>SRR765688.17960 HQP9GKT01CN8ZX length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGT
ACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGC
ATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCC
AAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACA
GCCACGTATTACTGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGGGGC
CAGGGAATCCTGGTCACTGTCTCCTCAGCATCCCCG
>SRR765688.25497 HQP9GKT01B88YS length=389|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAG
CAGTAGTAGTTGCTACTGGGGCTGGATCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATT
GCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATG
TCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGAC
ACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCT
CTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.39627 HQP9GKT01CATH4 length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNNCCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCA
TTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTT
ATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATAT
CAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACA
CGGCCACATATTATTGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCT
GGGGCCAGGGAACCCTGGTCACCATCTCCTCAGCCTCCAC
>SRR765688.48095 HQP9GKT01DC6RH length=389|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR4|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGC
TCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTG
GAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGT
CGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTG
ACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAGCGAGAGATGACTATACTTCGGGG
AGTTATTACGGGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCC
CCG
>SRR765688.32938 HQP9GKT01CBIO9 length=387|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTT
CTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACA
GCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGATCGGGGGCATGGATATA
GTTATGGTTATTCTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCC
CG
>SRR765688.33355 HQP9GKT01B9Y0F length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCT
CTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGG
CTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTC
ACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGC
AGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTC
TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.32244 HQP9GKT01C73BZ length=376|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGA
CATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTG
GATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAG
ACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGAC
ATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTC
CGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCATCCCCG
>SRR765688.17491 HQP9GKT01BUYPI length=378|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATA
CAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTG
GATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCA
CGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAA
GGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACA
GGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.13678 HQP9GKT01BUUUG length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAG
AGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATA
TATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCA
GCGGACACGTCCAAGAANCAGTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGG
CCGTGTACTTCTGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.7787 HQP9GKT01COO0Q length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGT
GCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTC
ATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACC
ATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCG
GACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAG
GGAACCCTGGTCACCGTCTCTTCAGCCTCCAC
>SRR765688.43055 HQP9GKT01ANQEB length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGAT
ACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAAT
GGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCC
ACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGA
GGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTG
CTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
>SRR765688.24232 HQP9GKT01BTWAC length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATA
CAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATG
GATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCA
CGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAG
GATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGC
TTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
>SRR765688.35786 HQP9GKT01DW0J7 length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTA
CACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTG
GATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAG
AGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAT
ATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGA
ACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.45263 HQP9GKT01CZ91S length=353|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACT
ACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTT
ACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACA
CGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTT
ATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAA
TGGTCACCGTCTCTTCAGCATCCCCG
>SRR765688.48840 HQP9GKT01BLP5E length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGT
GGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCAT
ATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCC
AAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACA
GCCACATATTATTGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGG
GGCCAAGGGACAGAGGTCCACGTCTCTTCAGCATCCCCG
>SRR765688.36695 HQP9GKT01BVA49 length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTT
CTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCA
CAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCA
GCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGC
CGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.13959 HQP9GKT01AER44 length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR4|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTC
AGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG
GTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGA
TTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGA
GCCGAGGACACGGCTGTATATTACTGTGCGAGAGTGGCTACAGATATACCCCAGGACGTC
TGGGGCCAAGGGGCCACGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.24180 HQP9GKT01CPLH4 length=353|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTG
CTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACT
ACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACG
CAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTG
CTGTGTATTACTGTGCAAGAGATTACAATTATGCTATGGACGTCTGGGGCCAAGGGACCA
CGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.43187 HQP9GKT01C588C length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTG
CTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACT
ACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACC
CAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGG
CTGTGTATTACTGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAA
TTTACTACTACTACGGTATGGACGTCTGGGGC
>SRR765688.46667 HQP9GKT01COT4Z length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNCCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGC
TCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTG
GAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGT
CGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTG
ACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAA
TACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.38963 HQP9GKT01C40UI length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGT
GGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGC
ATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCC
AAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACC
GCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTT
GACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.36155 HQP9GKT01BS5TB length=362|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTG
CTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCT
ACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATC
CAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGG
CTGTTTATTATTGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGGGGCC
AGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.18975 HQP9GKT01BH3GZ length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAG
TCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTA
TATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTC
ACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACAC
GGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCT
TGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.46037 HQP9GKT01APHCO length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTT
CTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATT
ACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACC
CAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGG
CTGTGTATTACTGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGGGGCCAGG
GAACCCTAGTCACCGTGTCCTCAGCCTCCAC
>SRR765688.15636 HQP9GKT01ATB6I length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGAT
GGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACT
CTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCAC
TAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACAC
AGCCACATATTACTGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTA
CTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.40959 HQP9GKT01BMXS4 length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAG
TGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACA
TGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATC
AGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACAC
GGCCGTCTATTATTGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGCGGCCA
GGGAATTCAGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.44149 HQP9GKT01CLVEF length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTT
CTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCA
GCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACG
TCTGGGGGC
>SRR765688.9210 HQP9GKT01ED1VA length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTA
CCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACT
ACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACC
AAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGG
CTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGT
GGGGCAAGGGAATCCTGGTCACTGTCTCCTCAGCCTCCAC
>SRR765688.31439 HQP9GKT01BFFKN length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAG
AGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATA
TATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATC
AGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACAC
GGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCC
CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.33456 HQP9GKT01BSJ79 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTG
GTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTG
AGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGG
GCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCC
TGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGC
TCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.40824 HQP9GKT01B3H0W length=380|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGG
CACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTG
GATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAG
AGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGAC
ATTTGAGGACACGGCCATGTATTATTGTGCGAGAGGCATTGGGAGCTACTACCGTGATGA
CGCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAGCCTCCAC
>SRR765688.25750 HQP9GKT01CM4A4 length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAG
TGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGG
AAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATAT
CAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACA
CGGGTGTTTATTACTGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGGGGCC
AGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.49935 HQP9GKT01BCQ7U length=391|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgM-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTG
CTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTA
CAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCC
AGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGC
TGTGTATTACTGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTG
GGGCCAGGGAAACCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCAACCCTTTCCCCC
T
>SRR765688.46955 HQP9GKT01BB4XL length=379|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTT
CTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCA
CATCACGGTTTGTCTTTTCCTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGG
CCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGC
ACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.41568 HQP9GKT01AV8YG length=399|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTG
CAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTG
AGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCC
AGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCA
GCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAGGTCGTCTTGTCCTGGTGGGA
GTTGCTATATGTCCGCCTCCTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCT
CACCATCCCCG
>SRR765688.24124 HQP9GKT01CMXTP length=364|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=8
NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGA
CTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGG
GCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCC
AAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTAC
TGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGC
CAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.32089 HQP9GKT01AO7NN length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATA
CAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATG
GATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCA
CGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAG
GGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGC
TTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.13860 HQP9GKT01AT8BJ length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTG
CTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGGAAGGACCTTCTA
CAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGC
AGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGC
TTTTTATTATTGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGGGGTCA
GGGATCCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.20740 HQP9GKT01ANHJ2 length=378|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTG
GATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTG
AGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGG
AAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCC
TGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGAAGGCTACGGTGGTAATCCCC
TACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC
>SRR765688.15114 HQP9GKT01DWQ8O length=372|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNATTGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACT
GGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCT
CATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTC
CAAGGACACCACAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAG
CCACATATTATTGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACG
TCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.44373 HQP9GKT01A960E length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAG
GAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCA
TATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATC
AGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACAC
GGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGT
CTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.22020 HQP9GKT01BU60O length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTT
CTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGC
TTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTA
CAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCG
GTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATA
CAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCG
TCTCCTCAGCCTCCAC
>SRR765688.23018 HQP9GKT01DGIK6 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGC
CCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTT
TGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCG
AGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGAC
CGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAA
TTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
>SRR765688.46548 HQP9GKT01BMT60 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCT
CTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCA
CAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACA
ACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGA
TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.11345 HQP9GKT01A2AIW length=375|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAG
AGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATA
TATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCA
GCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACG
GCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.43574 HQP9GKT01D10PE length=310|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgD-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTT
CTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCA
CAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCA
GCCTAAAGGCTGAGGACACTGCCGTG
>SRR765688.36594 HQP9GKT01B0W9F length=368|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTT
CTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCC
TTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCA
CAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCA
CTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGACGCAGTCGGGATCACGCCTCT
GGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.47496 HQP9GKT01DBCHA length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAG
TGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGA
TCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTAC
AGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACAC
GGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATT
TGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
>SRR765688.46707 HQP9GKT01BT4GG length=359|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTG
CTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGC
ACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGC
CAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGG
CTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGG
GAATCCTGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.35420 HQP9GKT01C0N6S length=397|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATA
CAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTG
GATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCA
GGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAA
GGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCATGGATTATACGGTTGTGATCATAC
CGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC
>SRR765688.35321 HQP9GKT01C64W5 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATA
CAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTG
GATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCC
ACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGA
AGGCCTCGGACACCGCCATGTATTATTGTGCGAGCCGCTCGGGTGACTACGGTGACTTCG
AGTTTGCCCACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAGCCTCCAC
>SRR765688.40878 HQP9GKT01B4JCW length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAA
TGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTA
TATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATC
AGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACAC
GGCCCTATATTACTGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGA
CTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAGCATCCCCG
>SRR765688.44256 HQP9GKT01DI3B9 length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR6|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTC
CTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCC
AGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGC
AGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTT
GCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGA
ACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTC
AGCCTCCAC
>SRR765688.42968 HQP9GKT01B3HAW length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGG
CACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTG
GATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAG
ACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGAC
ATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTA
CGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
>SRR765688.43118 HQP9GKT01AR4EN length=394|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGC
CCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTT
TGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGA
GTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACC
GCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAAT
TATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
>SRR765688.21808 HQP9GKT01A2WPP length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=9
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATA
CAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATG
GATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCA
CGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAG
GGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGC
TTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
>SRR765688.39792 HQP9GKT01C51DY length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGT
CTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGC
TTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCA
CAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCA
GTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGG
ACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
>SRR765688.39546 HQP9GKT01D90AI length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAG
TGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGA
AATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTC
AGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTC
GGGTATTTACTACTGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGG
GTCCCTAGATTATTGGGGCCAGGGAAGTTCGATCACCGTCTCCTCAGCCTCCAC
Raw
SRR765688.fastq
@SRR765688.30659 HQP9GKT01B1E02 length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTCGGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIHHHHHII@@@@IIIIIHHHIIIIHHHIIIIIIIIIIIIIIIIIIIIIBBBBIIIIHHHIICCDIIIIIIIIHHHIIIIIIIII@@@@IHHHIIIGGGIIIIIIIIIIIHHHIIIIIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIFI::::IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH6665IEE977HI455ICCGIIEEGIIIHHHDCCIIIIIDC
@SRR765688.38189 HQP9GKT01B1F8D length=357|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIICCCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBBBIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIDCCIIIIIIIIIIIIIIII77666=/@@@00--<<<==/2011>DFEEEBA?B7774=95000880227@DBGIEIIH????DHIIIIIHHHI
@SRR765688.23676 HQP9GKT01CBCVC length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIHHHHD@@==HHIIIIHDCCIIHGGHHIIIIIIIIIIIIIIIIIIHHHIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIICBA@HHIIHHHFBBEEECE5;///7<?BEE>>?DIIIIIIIIIIIIIII???HHHIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIICCCCIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIII>>>>IIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.44566 HQP9GKT01BUA3B length=393|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!;;;IIIEEEII???IIIIIIIIIIIIIIIIIIIIIIC=D999HIIIIIIIIIIIIIIIIDC????IIIIIIIIIHHHIIHHHIIIIIIIIEEEEEEE;;;;EEIIIIIIH>>IICCBBIIIIIIII@@IH;;;9@@EHB<77111=?BBIIEE????HIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHAAAIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIA>222<<5222@@GEE???IIIHHHIIIIIIIIIIIIIIII
@SRR765688.49541 HQP9GKT01ESY9C length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIGGGIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIII666HIIIIIIHH@@@@IIIIIIIHHHHHHIIIIIIIICCCAH??DHIIIIIIIIIIIIIHHHIICCCHHIIIIIIHHHIIIIIIIIIIIIIIIHHCCCD=ECCCECCCDCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIGE==4422@@@??D@@>??DCIIDDCHIIIIIIIIHHHHIIFI@
@SRR765688.45233 HQP9GKT01BVKNW length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCGGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!GGHIIIEEEII???HHIHHEIECABA=::00---.=AEEBCGIIIIIIIIHHHIIIIIHEEEEEDA@@/////=777@@@BEIIIIIIIIIIICCCCDHIIIIIIIIIIIIIHGGHIIIIIIIIIIIICC>>>>IHHGHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIH;;;HHIII@I>>>>IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAACCIIIIIIIIIII???IIIGGDIIIIIII>>==IGF999><999A@ACC@;=BEEGCCGBCCIIIII??
@SRR765688.17591 HQP9GKT01CLJHN length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNCCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC
+
!!!!!!!!!!!!!!!!!!IIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBBBIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIBBBIIHHHIIIIIIIIIIIIIHHHHIIHIIIIIIIIIIIHHHIIIIIIIIIIIIIIHHHIIHHHIHIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIFFFFIIIIIHFFFHIIIIIIIIIIIIIIIIIIIFEEIIIIIIIIIIIIIIIIIIIIHHHHHEEEHIIIIIIIIIIIII@@55566BIAGEEBDDIIH@<<<@DHHHIIIIIIII<<<9HIIGGGGD@6666B
@SRR765688.43976 HQP9GKT01CAKGO length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=14
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!477CEECCCIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIHHH@CBBIIICCCB<;;;;;78;;;IAAAIICCCCIIIIHHHIIIIIIIIIIIIIIIIIHIHHHHIIIIIIIIIIIIIIHHHIIHHHIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIDD;;;HHIIIIIHIIHHHHHHIIIIIGCCDHIIHEE====III???HH???HIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.49474 HQP9GKT01DTUWB length=384|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!???EEICCCIIDCCIIIIIIIIIIIIIIIIH????DHHHHIIIIIIIIIIIIIIHDD@A@@IIICBBE:777=/=22222=588EE==@BIIIIIIIIIIIIIIIIIIIIIIIIIIH666?DIIIIEEIIII66424;DECCIIIIIIIIIDDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHII????IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIHHHIIIIIIIIIIHCC666IIIIIHHHIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.34997 HQP9GKT01BF6LJ length=395|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCAACCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIICCCCIIIIIIIIIHHHHHHIIIIIIIIIIIIIIIIIIIIICCCCIIIIIIIIIIIIIIIHHHIIHHHIIIIIIIIICCCCIIIIIIIHHHIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIHHHHGGHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIHHHHIII>>>>H>IIIIIIIIIIHHHHHCEEI=DCCIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHH9555BBEA??>>BCC333C=IIIIIIHHHHHIIEEEFCF:
@SRR765688.33811 HQP9GKT01AZ9Y4 length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!==IIIIIIE;;;;IIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIBBAAIIIIIH????HHHIIIIIIIICCCIICCDIICCDIIIIIIIIICCCCIHHHIHHHHHII???IIIIIIIIIIIIIIII@@@@IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIICCCCIIIIICCCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.40396 HQP9GKT01DTRVH length=361|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!CIIIIIIIIIIIIIIIIIIIIIIIIIIIIII??==?IIIIIIIIIIIIIIIIIIB?4444?@BBCCEEIIIIHHHEIIIICC??????HIIIIIIIIAAAAIIIIDHHIHHHIH>HHCCCCIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIID???IIIICC;;;IIIIIIIIHHHIDCCHHIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBCCIIIIIIIIIIIIIIIIIIIIIIIIICCCIIIIICCCCIIIIIIICCCCIIIHHHIIIIIHHHIIIIIIIIIIIIIIIIII::::I
@SRR765688.38176 HQP9GKT01CL3CG length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!9;;IIICCCCEHIIHHHIIIIIIIIIIIHHBBCEE?66?444EBB@A@EDD999977<<=?<<69::5500002/22@@@>>BCCIEED?===330000005;BB=A;9<44=::::G==@@BEIBB8CC7779ICCDHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIH666?IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIB;;6666DHIIIIIBCCCIIIHHHIIIIIHHHIIICCGIIIIIIIIIIIICCCCI
@SRR765688.36681 HQP9GKT01B98GC length=382|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=13
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIFGIIIIC5555CCIIIEIEIICCCCCCCDHHIFHHIIIIIIIIIICCCCIIIIIIIIHHHHIIIIIIIIIIHHHIIIIIIIIIHHHIIIIIIIIIIIHHHHIIIIIE?;;;DHIIIHDCCIIIIIIIIIIIIIIIIIIHHHHHHI>GGHB@@@DCCIII?666DHIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII@@@@IIIIIII@@@@IIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.26204 HQP9GKT01AGSP2 length=365|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!CIIIIIIIIIIIIBBBBDDDHIIHDDDDIIIIIIEECCCCCIIEEEEECCCEIIIIHH????ABBBIIIIIIDCCIIIEBE:=<<77111177EEEEEEIIIIHCDCCIIIIIIIIIHHHIIIIIIIIIIIIIIIIII;;;;HHIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIAH4444EECCCIIIIIIIIIIIIIIIIIIIIIIICCCCICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.31319 HQP9GKT01B1IO7 length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!B@BDAA@<=2222;;EBBAEB@A?;555CCIIIIIIIIHCDDIIIIIIIIIIIIIIIIIIIIIIIIIICCCHHGGGIIIIIHDCCBB@@IIIIIHH<<<DHIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIDCCHIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHGGGIIIIIIIIIIIIIIIICCCCIIIHHHIICCCIIIIIIIIIIIIIIIIIIIIIIIHH
@SRR765688.20699 HQP9GKT01DRRB0 length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGATGGACCCAACTCCCCTCGACTACTGGGGCCTGGGAACCCTGGTCACCGTCGCTTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!I????>IIIIIIIIIIIIIIIIHHHIIIIHHHIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIHHHIIIIIICCCCIIIIIIIIII????AIII:555IEIIIIIIIIIIICCCIICC???GGDIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIHHHIIIIHHHIIHHHIIIIIIIIIIII=999IIIIIIIIIIIIIIIIIIIIIGG???IIIEEFB7744222;;9?4447;5==;=;22----223---..633:9==:7778<?AAEGEGGBBBAAIE
@SRR765688.48796 HQP9GKT01B94FW length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIIIIII???IIIIIIIIID???IIIIIIBB@@IIIIIIH666?HIII???IIIICCDHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHH???HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIICC???DIIIIIIIIIIIIIIIIIIIICCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHH
@SRR765688.41848 HQP9GKT01C3DX5 length=387|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCCGCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!EEABBIGBB:>::CCEIIEEHHHIIHHHHHHIHHHIIIIIDDCCIII>>C>HHIHH???CCIIIIIIIIIIIIHHHIIIIIIIIICCCBIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIII666HHHHHIIIIIIIIIIIIIIIIIIIIIHHHIIIIHHHIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIH???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHH>>>>ICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIII::::H
@SRR765688.23863 HQP9GKT01BPQLA length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCGCAACCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIII;;;;HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHD;;;;IIIIIIIIIHHHHIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIII???HIIIII;::IIIIIIIIIIIIIIIIIIII???HIIIIIIIII565;?DIHHHHIIIIIIIIIHHHG???HHIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIID??>@AAIIIHHHIIHGGHIIIIIIIIIH???HIIIIIEEHI?
@SRR765688.17127 HQP9GKT01CQJSD length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!E@>777<<94222@EIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHD???HIIHHHHHHHHIIIHHDDCHCCCCIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIHHHHHHIIIICCC99553>>HCCEDE566DDDDIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIEEG?95556DHIIIFIBCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.32432 HQP9GKT01CZ7DE length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!CDHIIIIIIIIIIIIIIIIIIIIIIIDCCIIIIIIIIIIIIIIIIIIIIHHHIIIHHHHHIBB@@IIIIIIIHHHICCDIIIIIII533EBBBGGGGA556DDHHHIIIIIHHHIIHHHHHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHD666?HIIIIIIIIII6;666DCDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.20304 HQP9GKT01AMKZ5 length=386|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIE;;;CAAGGGABBEEIEEEIIEGGICC???CEEEEIICC=CE?>>EEEEEE5559DIIIIIIIIIHHGHHIIIIIIIIGGGIIIIIIIIIHDCCIIIIIIIH;??HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIB67777999EEEEIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIHHHIIIIIIIIIIIIHHHIIIIIHHHIIIIIIHHHIIIIIIIIIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.47093 HQP9GKT01BTC88 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIICCCCIIIIIIIIIHHHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIICCCCIIIIIIIHHHIIIIIIIIIIIHHHIIHHHIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIGGGHHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHH==;?IIIIIIIIIIIIIID???HHHCCCIIIIIIIIIIIIIIIIIIIIIIIIIIFFICCIEEEHHD;??HIIIIIIIIIBB@@CCD???CCFFEIIIIII??;;9HIIIIIIIII@@@@I
@SRR765688.46353 HQP9GKT01CZULB length=366|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIICCBBHHHHHHHIIIIIHDCCCEEEEEDABBBBB999?EEEEEEEEGIIE====EEIIIIIIIIIIDBE==<<7712299GGEIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIE?5554AEGIBBEAC???HDCCDIIIIIIIIIIIIIIIIIIIEEEE:=4449BGIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBBBIIHHHIIIIIIIIIIIIIIIIIIIIIIICCCCICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIH@@AAI
@SRR765688.49658 HQP9GKT01D85J4 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!222E=;999BFECCCIIIIIIIIIIIIIHHHIIIIIIIIHHHIIIIIIIIIIIIIIIIC;;;;?IIHHHIIIIIHHHIIHHHIIIIIIHHHIIIEB?4444B>>=@CBEAEEC?5655EIIIDDCE<DEB5442;;?AB<22122?CCCIIEICDDIIIIIIH;;;IIIIIIIIIIIIIIIIIIIHHHIIIIIIIID666HIIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII;;;HHIIIIICCCCIIIHHHIIIIIHHHIIIIIIIIIIIIIIIIIIIIII
@SRR765688.31392 HQP9GKT01BBVPS length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=7
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IEEIIIIII@@@@IIIIIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIII?@?>=D???CIIIIIIIHCCCCHHHIIIIIIIHHBB>BDCCIIIIEEEEEHII9?444?FHHIIIIIIIIIIIIIIIIIIIHHHIIHHHCCDHIIIIHHHIHHHIIIIIIHHHIIIIHHHIIIIIIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAAAAIIIHHHHHCCCIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.11728 HQP9GKT01AL5DZ length=389|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIHGG6666IHIIIIIICCCCIIIIIIIIII;;;;HIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIII;;::HIIIIIIIIIIIIIICCCDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIID;???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHIIIIIIIIIIIIIIIIIHHHIIIIEE@DBD@77222277DADDCE???IIIIIIIIIIDDCEEEFID@=4474
@SRR765688.21411 HQP9GKT01CV52A length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNNGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!!FEBGFFDDIIIIIIIHGGGHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHIIIGA====@@@@FFHFGGGHHHIHHHIGGGGIIHHEGGGGGGC88555DGGFAAAHHIIIIIIIIIIIIHIIIIII@@@GIIIIHHHIIIIIIIHIIBBBGIIH;;;IIIIIIIIHIHIIIIIIIIFFFFFIHFFFIIIIIIIIIIIIIIIIIIGBBBIIIIIIIIIIIIIIIIIIIIIIIIIIGGGHIIIIIIIIIHGGGIIIBCCADHGGBDA7777AA55111>>EGFABCHIIIIIIIIIIIIIIIICCCCI
@SRR765688.46885 HQP9GKT01B8Z8I length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=7
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIBCAAHHHHHHHIIIIIHHHIIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIH????HIHHHHIIEIIIIIIIIICCCCIIIIIIIHFEGEIEICC2225?EEIIIIIIIIIIIIIIIIIIIHH===GGHIIIIIIIIHHHIIIHHHHIIIIIIIIIIEEEI??6666HHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAAAAIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIDC
@SRR765688.15716 HQP9GKT01BPKZR length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGAATGGACTACACTCATTACCATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!CCDHIIIII@B@@HHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCBIIIIIIIHHH???HHHHIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIH??7ECCCC555IIIIII???IIIIHHHIIIIIIH????HHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.28724 HQP9GKT01CM7HO length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIICCCCIIIIIIIHHHIIIIIIIIIIIHHHIHHHIIIIIHHHIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIDD666CCIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIDDDHIIIHEEEHIICD53;CIIIIIHHHHHHHIHHHIIIIIIIIIIHCDDDHIIIIIIIIIIIIIIIII>>;;;;HEEEEIIIIIIIIIIIIHHHHHHIIIIIII@B@@;
@SRR765688.35930 HQP9GKT01CJLTL length=406|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIII6666IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIHH;;;;IIIIICC;;;HIIIIIIIIIIHHHIIIIIIIIIIH;;;IIIIIIIIIIIII666IIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHGCD=HIIIIIIIIIIIDCCHHIIE=5586?@00///2215@DEEEIIHHHIIIIIIIDDDDFFEGEEDA;;=2222<>>>BBBAGIII???IH;;;?IIIIIECCBGI@@A;
@SRR765688.30106 HQP9GKT01CBSGA length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGACTTGACGGCAGCGATAGGGACTTTTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!??IIEHHHI;;;;HHIIIIIDCCIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHIIIIIHHHHIIHHHIIIIIIIII@@@@HHIIIIIHHHIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII>>666;;HIIIIIIIIIIIIIIIIIBBBIIIIIIIIIIIIIIIIIIIIIIIIIIHEEFC?>?B4444::<<EEFCDGCC???IIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.42360 HQP9GKT01B8L6A length=364|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCAAGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIII???>>IIIHHHIIHHHIIIIIIIIIIIIIIIIIIHHHIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH???IIIIIIIIIIIIIIIIIIIIIIIIIIHHHHIIIIIIIIDDDIIICCDIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIIBBBBHHH???IIHHHIIIIIIIIIIIIIIIIIIIEB42222
@SRR765688.38856 HQP9GKT01A7EJO length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIDDCC@@CCIIIIIIIIHDDDHIIIIIIECCDHHCCCCIIIIIEECBBEEE???::::EEGEEE???CCIEBE@=<<??2235??IIIIEEIIIII?;666IIIIIIIIIIIIIIIIIIIDCCCIIIHHHIIIIIIIIIIIIIIIIIIIICCCIIIIIIIIIIIIIIIIIIIIIDD666DIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIICCCCIIIHHHIIGGGIIIIIIIIIIIIIIIIIIIIIIIDC
@SRR765688.23240 HQP9GKT01BYYSD length=378|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!II===@;;;?ICA777@@55597AEGEEIGH???HIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIHGGHIIIIIIIIIIIIIIIIIIIIHHC??;IIIIGGE?4449B999EIIIIIIIIIEIGB44599544===>BB92:000020:998?FABAGECC566DDIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII;;;;IIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.45110 HQP9GKT01BTIR2 length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!CIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHHH???CIIEEEEE???E;;;;EEGEEIGEE?>><CE:=<<??222AAGGIEECCDHHHHIIDCCHHIIIIIIIIIIIIIHHHIIIIIIIIIHD?>>>>IIIIIIICCCCIIIIIHHHIIIIIIIIIIIIIIIIIIIIIII666DIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIE<=////74474BEEA???DIII@@@@IIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.41920 HQP9GKT01DGU7R length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!BEIIIIIIE=@@@HHIIDDDIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIID???D???IIGEGIGEII?555;?IIIIIIIIIIIIIIIIII???IIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIHH;;;HHHHHHHHIIIIIIHHHHHHIIIIIICB?BB99;;;CEIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBCCIIICCCIIHHHIIIIIIIIIIIIIIIIIIIII::::G
@SRR765688.5099 HQP9GKT01AS88P length=374|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!EEEBBBEEB4444AAEEGEIEIIIIHGGHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIHHHIIIIIIIIIBBBBIHHHIIIHHHIIIIHHHIIIIHHHIIIIIIIIIIIGGGHHHIIHCCBBHHE????DHIIHHHIIIIIIIIIIIIIIIIICCCDIIIIIIHHCCCCDHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.41370 HQP9GKT01DK3O2 length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNAAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIEEEEE>?9999==GIIIIIIIIIHHHIIHHHIIIIIIIIIIIIIIIIIDCCHGGHIIIIIIIIIID???CCIIIEE@AEEEEE4445?BEEIIIEEI;??IIHHCCDHFHHIIHHHIIHHHIIIIIIIIIIH????HHIIIIIIHI@@CCHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIAAAAICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIII??
@SRR765688.46467 HQP9GKT01EZF79 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!EEGIII6666IIIIIIIIIIIIHHHIIIIIIIIIIIIIICCDHIIIIIIIIIIIIIIIIIIIIIIIIIIH666DIIIIIHCC@@BBIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHHHIIIIIIIIIH???CCIFDDIIICCDCIEEA?999>C555CCIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHIIIIIHHHHIIIIHHHIIIIDDDCCIIHDDDIIIIIIIIIDDDDHIIIIIIIIIIIHHHHIIIIIHHHHHIIIIII@BBBCC????HIIIIIIIIIIIIIIIIIICC???DI@@;;;
@SRR765688.17960 HQP9GKT01CN8ZX length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIH?6666IIIIIIIIIIIIIIIHHHIIIIIIIHHHIIIIIIIIIIIII;;;HIIHHHIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIHHHIIIIIIHHHIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHH666HDHHIIIIIIICCCCIIIIIIICCCCEEIIHH???IIIIIIIIIIIIIIIIIHHHHHIIIIIIIIIIIIID???IGCHII????HHHII@@@@IIIIIII@@@@IIHCCEIIICCDHHHIIIIIIIIIIIIIIIII;;422
@SRR765688.25497 HQP9GKT01B88YS length=389|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHHIIIHHHIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIICCCIIIA@@@IIIE752:==55-----2..3DD:<@@IIIIIIIIIGGGIIIIIIIIIIIIIIIHHHIIIIIIGGGIICCCA??II666?CGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIGGDI666??CGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGHHHIIIII;;;?GFIIIIIIE675?DEEEBA==3::888>>FACCC@@@@IIIHGGGGGGHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.39627 HQP9GKT01CATH4 length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNNCCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!!999HHIIIIIIIIIIIIIIIIIIIIIIC???DDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHI@@@=IIIIHH???IIHHHHHHIIIIIIIIIIIIIIIIHHHIHHHIIIIIIIIIIIIDD:CC>>>>IEEEIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHH???HIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCDHIIII;;556DIIIIIIIIIIIHHHIIIEEE<?94424?:<==IIIHHHIIHGGHIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.48095 HQP9GKT01DC6RH length=389|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR4|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!IHHHIIHHHHHHIHHDGGIIDCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII??????CDIIIIIIIIHHHIIIIEDDA4>2221///77BBBGIIIIIIICCDIIIIIIIIIHHHHIIIIIIIHHHHHHHHHHIIIIIII?77;HHHHIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIEEEEEEEE111179BBBBBCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIIIIIIIHHHHHEIICCCCICCCCIIIHHHHHGHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.32938 HQP9GKT01CBIO9 length=387|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIICCCCIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIHHHIIIIIIIIIBBCCIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIHHHHIIIIIIIIIHHHIIHGGHHIIIIIIHHHIHHHDCCIIIIIIIIIIHHGHIIIIIHHHIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIICCCIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIID77777IIIIIIIIIIIIIIIIIIIHHHHIIIIIIIIBBBBIIIHHHIIGCCDIIII???DIIIIIIIIIIII@@@@?
@SRR765688.33355 HQP9GKT01B9Y0F length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!22=>EGIEH@@BCIIIIIIIIIIEE?;;99BEBBBIIIIIIIIIIIIIIIIBCCCIIIHHHIIIIIIIHHHIIHHHIIEHCEE<<///--59A=BADBBCHHEII?E112C566?HHHHIIIIIIIIIIIIIIHHHIIHHHIIIIIIIICCCIHHHIIIIIIIIIIDCCCCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIH;;;HHHHHIIIIIIIIIIIIIIIIIIIIIAAAAI
@SRR765688.32244 HQP9GKT01C73BZ length=376|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHGHHIIIIIIIIICCCCIIHHHIIII??;IIIIIIICCDCI====EIE????HIIIIIIIIIIIIHHHIIIIIIIIICCCCIIIIIIIIIIIIIIEEEEIE?222?CFFIIIIIHHHIIIIHHHIIIIIIIIIIIIIIIIIIIIIHHH;;;IIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIII@@@@IIIIIIIIIIIIICCCCIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.17491 HQP9GKT01BUYPI length=378|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!HH@@@@IIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIHHHHHHHGGFFIIIIIIIIIIII;;;;H>>HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHFDIIIIHHBBCCHHIIIIIIIIIIIIIIIIIIIIHHHHIIIIIIIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII=999CCIHHHIIHHHIIIIIIHHHI?????BEIIEEEEIF
@SRR765688.13678 HQP9GKT01BUUUG length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!775B53///=60006?@CDBCBEEGEE?=775=777AIGIIIIIIIHHHIIHHHIIIIIIIIIHHIIIA8888D===DD555FAAAIHGIHHHGGGGHIIIIIIIIIGGGIIIFGB>>>BBBDFGGE?DD222F888=;EEGIHIIBBBGGIIIIIFFGIIBBBGFFFGHGDA?;51!115///::;?::8;;>@?C667?GGFHIIIABBFDDFDD;;;;;==ADGAABCAGGEGECG@72/1199?5444ACEDD>>@<@::0--97//2--29///9488<HIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIBBBBI
@SRR765688.7787 HQP9GKT01COO0Q length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!HIIIIIIIHHHHIIIHHHHIIHHHIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIHHH=;=;;IIIHGGHH;;;IHHHIIIIIIIIIIIIII666?CIFEFIIIII???HHHIH;;;?IIIIIEIB555?IIIIEEEIIIIIIIIIIIIIIIIIIIIHHHHIIIIIIIICCCCIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIID;;;HHIIIIIIIIIIIIIII>>>>IIIHHHHHGGHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.43055 HQP9GKT01ANQEB length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!HHCCCCIIIIIIII66666IIIIIIDDCIBBCCIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIH666?IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIIIIHHGHHIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIII;;;DHIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHCCDHIIICCDHIIIIIIIIIIHHHIIIIIIIIIIIIIIE?999::99999>BCEEECHHHIIIIIIIIII???DIIHHHIIIF
@SRR765688.24232 HQP9GKT01BTWAC length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!2499::IIIIIIID@@@@HHDCCH?IIIAABAIIIIIHHHIIIIHHHIIIIIIIIHHHHIIIIIIIHHGHHIIIIIIIIIHHHH=????HHIIIIIIIIIIIIGGGIIIIIIHHHIIII???HIIIIIIIHHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.35786 HQP9GKT01DW0J7 length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!EB5555EEIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII???IIIIIIIIIIHHHIIIIIIHHHIIIIIIIIICCCCIIIHDCCGGHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHGGGIIIIIIIIII???IIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHCCDIIIDDDHI???HHIIIIIIIIBBBB???CEEIIEECC;;;DIIIHHHHHIIIIIIIIBBBB?
@SRR765688.45263 HQP9GKT01CZ91S length=353|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!HIIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIHHHIIIHHH:::IIIIIIIIIIIHHHHIIIIIID>>;;;HIIIIIIIIIIIIIIIIIIIIHHHCC777HIIIIIIIIIIIIIIIID;;;IIIIIIIIIIIIIIIIIIIIIIHHHIIHHHIIIHHHIIIIIIIIIIIIIIIHHHH???CIEBBACE???D55;DIIIIIIIIIIIIIIBBB@H>HEIIIIIC?;;;;BGIEEE@99<<CCC<??EIHHHHIIIIIIIIIDDDHIIIIIIIBBBBH
@SRR765688.48840 HQP9GKT01BLP5E length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIH????DIIIIIIIIIIIIIIHHHHHIIIIII??????CIE6?>BBIIIIEE==?EEBB666634;;<<<572227@AAAADGIHH;;;HIIIIIIIHH666?DIIIIIHHHIIIIIIIHD666DCCIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHCCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBBAAIIIIIAAAAIIIIIIICCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIII@@@@I
@SRR765688.36695 HQP9GKT01BVA49 length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIHD@@@@EEHIIIIHCCDHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIHHHIIIIIIIIIHHH????CHIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIII?;;;HIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.13959 HQP9GKT01AER44 length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR4|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAGTGGCTACAGATATACCCCAGGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!DD<<DBBBA555DGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGG<<<GG@@@@DDDIICCB==556BIIIIIIIIIIIIIIIIIIIIIIIIIIGBBBGIIIIIIIIIIIIIIIGGGGGIIIGBBBGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIGGGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCBBIEADBE?99////44994888EIIIIIIIGGGGIIIIIIIIIIIIIIII
@SRR765688.24180 HQP9GKT01CPLH4 length=353|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGATTACAATTATGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAACCIIIIIIIIIIIIIIIIIIIIIHHH??<<HIIIIIIIIIIHDCCIIII5555DI@IIIIIIIIIIIIIIIIIIH;;;;DIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHIHHHIDCCHIIIIIIIIIIIIIIIIIIIIHHHII???DIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHDDDHHHABAAIIIIHHHIIIIIIIIIIIIDDDDCEEEEEEB977?;
@SRR765688.43187 HQP9GKT01C588C length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGGGGC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIICCCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAAAAIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHHIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHCDDHHHIIIIIIIIHHHIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHI==?HIIIIIIHHHHIIIIIIIIIIID???CICCCCHIIIIIIIIIIIIIIIIIIIIIDDD@@@@I
@SRR765688.46667 HQP9GKT01COT4Z length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNCCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!??>>A@AEA9999CCCCCCIIIIIIIIDDDIIIIIIIIIIIIDCCCIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHDGGHIIIIIIHHHIHHHHHGGHIIIIIGEECCC??544=AIIIIEIEHHIIID;;;HIIIIIIIIIHACCAAHIIHHHIHHHIIIIH???BBGIIIIIIIIIIIIIIIIIIIIIHHHIIIIIHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHIIIIIIIIIIIIIIIIII9;9CCHHIIIHHHACCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIII<;
@SRR765688.38963 HQP9GKT01C40UI length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHCCCCCIIIIIIHHIIIIIIICCDIIII;;;;;IGGGIIIIIGGIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIII;666DHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIICCCCIIH;;;IIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.36155 HQP9GKT01BS5TB length=362|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAAAAIIIIIIIIIIIIIIIIIIIIIGGGIIIIIBBBBIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCHIIIIICCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIII;;;;;II<<IIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIDCCIHHGHHICCIIIIIC?977=C=722293EIIIEEFEEGEE5555555IIIIIII@@BCHCD??;HHFFHIIIIIIIIIIIIIIIIIIIIIA@==E
@SRR765688.18975 HQP9GKT01BH3GZ length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!222=BICCCII????IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIC???EIIID<<<=HHHHIIHHHIIIIIIIIIIIHHGGHIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIICCCCIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHIHHHIIIIIIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIAAAAI
@SRR765688.46037 HQP9GKT01APHCO length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIII>>>>DIIIIIIIIIIIIIIIIIIII???IIIIIIIIH::;DIIIICCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII?::;;IIIIIHGGHIIIIIIIII==>>>II;HHIIIHHHHHHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIEEE<;222275=557BBEHGHIIIIIIIIID?????IIIEEEEII
@SRR765688.15636 HQP9GKT01ATB6I length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIH???IIIICCCCEII?;;;EEIICCCIHHCCDHIIIIIHHDDCCHIIIFFHIIHHHIIEGEED@//////==>BDIGIFHHIIIIIIIIIIIIIIIII>HHBBBBFDDHHHIIIIIIIIIIIIIIIIIIIIIIIIIIHHDDBBHIIIIIIIIIIIIIIIIIIIH;99?CIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIHHHIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.40959 HQP9GKT01BMXS4 length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!955AGECCCIB;??IIIIIIIIIIIIC?????DHHHIIIIIIIIIIIIIIIH????HHHIIIIIIIIII@@@@D===IIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIAADIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.44149 HQP9GKT01CLVEF length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIICCCCIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIA@=<GGGEB33445CCIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIGGGHCC@@CC;HIIIIHHHIHCC;;;IIH????HIIHHHIII???HIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHH??;;;HHIIIIIIIIIIIIHHHIIIIHHHIIIIIIIIIIIIHHHI=????HHHIIIIEG@@....-5
@SRR765688.9210 HQP9GKT01ED1VA length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIBBBBIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIICCCCIIIIIIIIIIIIIIIIIIIHH???IIIIIIIIIIIIIIIIIIIIII@??;;;;HIIIIIIIIIIIIIIIIIIIIIIIII???CDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIICCCBIIIIIIHHHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCIIIIIAI==>>III666HHIIIIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.31439 HQP9GKT01BFFKN length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHHIHHGHHH????IIIIIIIIIIIIIIIHHHHHHIIIIHDCCIIIIIIIIIIIIIIIIIIIIIIIIIIBBBBHA==????CIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIB4;;3333E???IEEHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH>>>>C???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIIIIIIIIIHHEII@@@@ICCCCIIIGGGIIHHHHHHIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.33456 HQP9GKT01BSJ79 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIAAAAIIIIIIIIIIIIIIIIIIIIIIIIIIIII????IIIIIHHHIIIIIIIIIIIIIIIIIII666HIIIIIIHH@@CCIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH;;;HIIIIIIIIIGGGIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIHCCDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIHDD@@BBCCDCHHIIHHHIIIIIIIIIIIIIIIIIIEEE::999
@SRR765688.40824 HQP9GKT01B3H0W length=380|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!II666GIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIHHHIIIIIIIIIIIIIGGGIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIHHHIHHHIIIIIHHGH888CCHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCHIIIIIIIIIIIIIIIIHHHHHHIIIIIIIIIIH??<<<;9IIIIIIIIIIIIIIIHHHACBBIIIIIHD>CCCCCCCHHHHIIIIIIIIHCCDHHH???DCIIGIFFIF
@SRR765688.25750 HQP9GKT01CM4A4 length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!CCCDDHFEEIIIHHHIIIHHHIIIIICCC;;;??CCCEI?IEEFIIID???HHEECCBEEEIHHHDDDD;@@@=IHHHIIHHHIIIIIIIIIIICC@===;;;IIIIHHHIIIIIIIII;;;;HHIIIIIIHHHIIHHHIIIIIIIIIIIIIH;;;IIIIIIIIIIIIIIIIIIIIIIIIICCCCI?666HIHGGHIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIHHGHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIICCCCIIIHHHIICCCIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.49935 HQP9GKT01BCQ7U length=391|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgM-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCAACCCTTTCCCCCT
+
!!!!!!!!!!!!!!!!!!!?IIIIGGIE;?884455IIIEEEIIIIIIIIIIIIIIIGGGIIIIIG???HFFIGGGG????@@@@???IIIIIIIIIIIIEBBB88223388EGEECFIEGEE88444><><<;@CCEACCEEGIIGCCCIIIIII???HIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIGGGHIIIIIIECCGGIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIG??;;;;GIIIIIIIIIHHHIIIIIIICCCCIIIHHHHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIII??I>>>44111CCC7779;I
@SRR765688.46955 HQP9GKT01BB4XL length=379|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIICCCCIIIIIIIIIIIIHHHGGFIIIIIIIIIIIIIIIIIIIICCBII?666?7IIIIIIGGGIIHHHIIIIIIICC@@BBIIIIIIIHHHIIIIIII:::H444?EIIIIIIIG:;;IIIIIHC@444444....5447?GGIIIII???IIIIIIIDD<<:6/5////44.99FECEIIIIIIIIIIIIIIIIIIIIIIIIIIGEEEIIIIC;688?CIIIIIIIIIIIIIIIIIIIH?=><ACCIA833557CIIIIIIIIIIIIIIIGGGGIIII???E=@@@GCC;;;IIHHHIIIIIIIIIIGGGIIGGGIE?;;CE
@SRR765688.41568 HQP9GKT01AV8YG length=399|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCACCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIHHHIIHHHIIIIIIIIID???HIIIIIIIIIIIIIIIIIHDCCGIIIIIIIIIIIHHHIIIIIIIIIIIIIIIICC5555EEIIIIIEIHIIIIIIIIIIIHHHIIIIIIIIICCCCCIIIID955CCHHHIIIIIIIIIIIIIIIIIIIIIIIHCCCIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHI???IIIIIIIIIIIIIIIHH666HIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIAAAAI
@SRR765688.24124 HQP9GKT01CMXTP length=364|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=8
NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!HHHIIIIIIIIIIIIDCCHIIII99;;IIIIIIIIIIIIIIDCCIIIIIIEEGB?911111?===EEIIIIIIHHHHHIIIIIHC;;;DIIIIIIIIIBBBBIIIIHHFIIIIII:;;9999IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIHH????HHHHIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIHHHHIIIIIIIIIIIIIIIII?;;;HHHII@@@@HIIIIIICCBBIIIHHHIIIIIHHHIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.32089 HQP9GKT01AO7NN length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!CC>>>:EECCCFA722427:?BBA6C@C5555EIIEEEGEEHHH???EIIIIIII????HIIIIIIHHHIIIIIIIIIIIHHHI==???HHIIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII@@@@IIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIDC
@SRR765688.13860 HQP9GKT01AT8BJ length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGGAAGGACCTTCTACAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!CIIIIIIIE?B9722228=B888=:EIIIIIIIIIIIIHHHIIIIIIIIICCDHHHII???H;;;;;IIIIIHHHHHIIIIIIII;;99;;IIIIIIIIIIIIIIHHHHHHIIIIIIIIIIIIIIIIIIIIIIIHGGHABCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIDDCCDIIIIIIIIIHHHIIIIIIIIIHH88888HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHDCCGHHIIIIIBCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.20740 HQP9GKT01ANHJ2 length=378|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIBBBB>>>=IIIBBBFDHIIGGGGEDA?;<444<DEIIIIIIIIIHHHIIIIIIIIIIGGGIIIIGGGDBBBDDGDDBBBIIIIIIIF>>>=DGEEGEA87797EGEEEGHAD>>>DGGGIIG=::::>@@=GI9GGB===<;A==;;<BGGIIIDCDADE=B;5,,,,-
@SRR765688.15114 HQP9GKT01DWQ8O length=372|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNATTGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCACAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIC?555IIEEHHIIIIIIHHHIIIIIIIIIICBCCCI????EEEEIIIDDDIIIIIIIIIIII?;/?:;=::??GHHHHHHHHIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIII;;;IIIIIIIIIIIIIIIIII???HIHHHIIID;<<AB:2221055?9::5.20111<<A@@EEIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIICCCCIICCCCIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIHH
@SRR765688.44373 HQP9GKT01A960E length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHHIIIHHHIIIIIIIIIIIIIIIIIIIIIHHHHHIIIIIIIIIIIIIIIIIIIIIIIHHHHIIIIEE333<<D=?@IIFHHIIIIIIIIIIIIICCCIIIIIIIIIIIIIIICCCCIIIIIIIIIIIIIABBBIHHHIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIICCCCIIIIIIIIIIIIIIIIIIGGGIIIIIIIICCCCIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.22020 HQP9GKT01BU60O length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!@<?555555000088ADDEEIEEECCBEIIIEC;;;;CIIIIIIIIHHH@@CCIIIGGGIIIIIIIIIIIHHHHHHIIIIIHHHHB@==C???EI;;;;EEEIIIIIIIIGCCDIIIIIIIIIIIIIIIIIIBBBBIHHHIIIIIIIIHHHIIIIIHHHIIIIIIIICCBBIIIIIIIIIIHHHIIIIIIIIIIIIIIDDCCHIIIIIIIIIIIIIIIIIIII?666??IIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIAAAAIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@SRR765688.23018 HQP9GKT01DGIK6 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!HHHIHHDDDIIHHHIIIIIIHHHHIIIIIIIIIIID==D???IIIIIIIIIIIIIIIIID???HIIIIICCDDHAAAIIFFFIHHHIIIIEEGGGE94442=@==B@@=B11;;@@@@IIIIIIII@@I?;;;;EBEHB;791222CCGGIIEIDHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHGDCCIIIIEC555EIIIIIIE???IIIIIH;;;HIIIIIIIIIIIIHHHHHHIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIII>>>>IIIHHHIIIIIHHHIHGCCIIIICCGIIIIIIIIHH
@SRR765688.46548 HQP9GKT01BMT60 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIICCCCIIIIIIIHHHIIIIIIIIIIIHHHIIIIIIIIIIHHHIIIIIIHHHIIHCC667?IIIIHHHIIIIICCDIIIIIIHHHGDDIIIIIHD;;;IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH===IIIIIIIIIIIIIIIIIIIIIIIDDDIIEIIH=DDHIIIIIIIIIIIDDDIIIIIIIIIIIIAAAAIIIHHHIIHHHIIIIIIIIDDDHHHEEEEGGG;=;7
@SRR765688.11345 HQP9GKT01A2AIW length=375|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHHIIIHHHIIIIIIIIIIIDDDHIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHHHIIIB@@=I?==??;;;HHIIIIIIIIIHHHHIIIIIIIIIIIIIIIIIIIHHHIIIEEE>:577//1E335EEHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGEAA=2111175<?=BDDIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIH???BBHHHIIIIIIIIIIIDBBDCCEB;;771>922298<<@IIIDHHIIHHHIIIIIIIIIIIIIIIIIIIIIBBBBI
@SRR765688.43574 HQP9GKT01D10PE length=310|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgD-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG
+
!!!!!!!!!!!!!!!!!!!!IIIIIIIIIFFFFIIIIIIIIIFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFDDDDFFFFFFFFFFFFFFFFFFFFF:::ADFFFDDDDDACCCADA:98;<>>888443=B@AA=:::??==AAA<<<444555:8;A>BBDDD::::DBBA::::=AADDBAAAAAACFCDCCDDFFFFFFFFFFFFFF@@@GGIIIIGGGGGIIIIIIII
@SRR765688.36594 HQP9GKT01B0W9F length=368|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGACGCAGTCGGGATCACGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!EA8555=95000088?AA==:<>BCBBCEEEII????IIIIIIIIIIIIHHHHIIIIIIIIIIIIIIIHGGHHHHHIIIIIIIII@@@@IIIIIIIII;;;;;;BIEEGGEEFIDCCDIIIIIIIIIIIIIIHCCCCHHIFFHHDHIHGGHIIIIIIIIIIIIIIIIICCCIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII:;666??FHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIII666DIIIIIIIIIIIIIHHIHIHHHIIIIIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.47496 HQP9GKT01DBCHA length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!HHHIII???IIHHHHIIIIIDDDIHD==IHHHI????IIIIIIFHHIIIIIIIHHHHIIIIIIIIGGEB99999444BB<@@@IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII;;;HIIIIHHHIIHHHIIIIIIIIIIIIIIIIICCCIIIIIIIIIIIIIIICCCBIIIIIIIII666HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHCCCIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIHHHIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIICCCCI
@SRR765688.46707 HQP9GKT01BT4GG length=359|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIHHHIIIIIIIII;;;;IIIIIIIIIIIIIIIIIIIIIHHHIIFFHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHIIIIIIIIIIIIIIHHHHIIIIIIIIIIIIHHHIIIIIICCDHIIIIIIIIIII???IIIHHHIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIC?==HIIIIIIIIIH????IIIHHHIIIIIIIIIIIIIII?????HIIIIIIIII
@SRR765688.35420 HQP9GKT01C0N6S length=397|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC
+
!!!!!!!!!!!!!!!!!!!!BE===BIIIIIIIIIIIIIIIIIIIIII???HIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHHHHHGGFFIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII???IIIIIIIIIIIIIICCDHIIIEEEEHDDGHHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGHIIIIIIHHHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIII???HEEDEF@?8800005
@SRR765688.35321 HQP9GKT01C64W5 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!;;AACBIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIBBBBIIIIIIIIIIIIIIIIIIIHHHIIIICCCCCCHCCCCCEDCCAAIIIIGIBIBBA@//---5-2;AB>ABHHHIIIIIIIIIIIIIIIIIIIIIIG999C?77??BBIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHHHIIIIIIIIIIIIIIIIHHHHIDCCIIIIIIHD???HIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIDDDDHHHIHHHIIICCCCIIIHHHIIHHHIIIIIIIIIIIDCCDHIIIIE==?C
@SRR765688.40878 HQP9GKT01B4JCW length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!997IEECC@EC???IIIIIIIIIIIIIIIIIIIHHHIDCCHHHIIIIIIIIIIIIIIIIIIIIIIIIIIBBBBIIH=????HIIIIIIIIIIIHE???IID????HIIIIHCHHHHIHHHIIIIIIIC;;44?BA==;ADBBBG<A=555?EIIIIIIIIIIIIIIIIIIIIIBBDHIIIIIHHIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIHHHIIIIIIICCCCIIIHHHIIHHHIIIIIIIIIIIIIIIIIIIIIAAAAI
@SRR765688.44256 HQP9GKT01DI3B9 length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR6|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!<<<<<IIIIIIIIIIIIIIIIHHHIIIIHHHIIHD???IIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIHHHAACCIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIDCCHIIIIIIIIIIIIIIIIII??666CCIIIIIIIIIIIIIIIIIHHHIIIIIIIIHBBDIIIIIIIIHHHII???IHHHHIIIIIIIHCCDIIIIIIIIHHHIIIIDDDHIIIIIIIIIHHHICHD???HHIIIIIIIIIIIHHHHHHHIHHHHHIHHHB@AAHHHHHHFIIIIII????CIIHDCCCIIIEEAD@@74
@SRR765688.42968 HQP9GKT01B3HAW length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=5
NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
+
!!!!!!!!!!!!!!!!!!!!IIGGGIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIC?555;7IIIIIIIAACCIIIIIIIHHHIIIIIIIIIIIHHHIHHHIIIIIHHHIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIICCCCIHHHIIIIIIIIIII???IIIIIIIIIIIIIIIIIIIIIH??==;DIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIHHHIHHHIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIICC@@HHIIHHHI???DHHHHIIIIIIHHHIIIIIIIBBBB?
@SRR765688.43118 HQP9GKT01AR4EN length=394|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=4
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!774EEFCCCIB799HIIIIIIIIIIIHHHIIIIIIIIIHGGHIIIIIIIIIIIHHHIIIDCCDIIIIEGEEG?211199BBBGGGIIIIIIIIIIID??;;EIIIIII?;22??BBBBIIIIIIIH>>II@@@=EEGIIC;;222?CCCIIII???IIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHHHHIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIICCCCIIH666IIIIIHHHIIIIIIIIIIIIIIIIIIIIDC
@SRR765688.21808 HQP9GKT01A2WPP length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=9
NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!GG@@@@IIIIIIIICCCCIIIIIIIIII>>>>IIIIIIIIIIIIGGGIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIHHHIIIHHHIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIICC5544CEEEHHHIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIH;666?IIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIBB@@???CHHIIHCCDHHIIIIIHHHHIIIIIIIIIIIII
@SRR765688.39792 HQP9GKT01C51DY length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!?=EGDEEB?4444EEGIIII???IIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIEEGBBB993337;ABBFFAE5<<<HIIIIIIFGGHHHIIIICCDIDDDIIHEEED@@<<@;;4449EEEEIIBEHIIIIIHHHGGHIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIHH666?IIIIIIIIIIIIIIIIIIIIIIIIIIIIH;;;CCCCBI;;;;IIIDDHIIHHG???IIIIIIIIIIIIIIIIIIIIII
@SRR765688.39546 HQP9GKT01D90AI length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCAGGGAAGTTCGATCACCGTCTCCTCAGCCTCCAC
+
!!!!!!!!!!!!!!!!!!!!777<<C@@CIAB====DIDDBBB>>>GIIIIIIIIIIBBDGGDAAIDDGGGGDIIIIIIBBAADB===D===>>26:II====>>>>BBBDIGEEDA7===BDDDGGGD==<BGFF===EEC@@?54111///7;@@@EHGIIIIIIIIIIIIIIIIIIIIIIIIIIIGGGBBBABA?BBEE==7D>>>IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHFG;;;IIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIHHHIIIIIIIIIIHHB;666;>IIGGIIIAAAAIIIHHHIIIDDDIIIIIIIIIIIIIIIIIIIIIIII
Raw
SRR765688.fmt7
# IGBLASTN 2.2.29+
# Query: SRR765688.30659 HQP9GKT01B1E02 length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TTCGA CCCTCCAACCGCGTATT GCAGCA CCTGAATT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 23 62 40 39 1 0 97.5
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 48 3 0 94.1
CDR2-IMGT 138 161 24 22 2 0 91.7
FR3-IMGT 162 275 114 104 10 0 91.2
CDR3-IMGT (germline) 276 279 4 3 1 0 75
Total N/A N/A 257 234 23 0 91.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.30659 IGHV7-4-1*02 91.05 257 23 0 0 23 279 36 292 9e-93 330 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG24TCTC7AGAC3TG1AT4GA44AGCG10TA6TG8TG2CT7CG21TC15AG35AG4CG7GC1AG7TC3TG3
V SRR765688.30659 IGHV7-4-1*01 90.66 257 24 0 0 23 279 36 292 8e-92 327 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG24TCTC7AGAC3TG1AT4GA44AGCG10TA6TG8TG2CT7CG21TC15AG23AT11AG4CG7GC1AG7TC3TG3
V SRR765688.30659 IGHV7-4-1*04 90.66 257 24 0 0 23 279 36 292 8e-92 327 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG24TCTC7AGAC3TG1AT4GA44AGCG10TA6TG8TG2CT7CG21TC15AG6CT28AG4CG7GC1AG7TC3TG3
D SRR765688.30659 IGHD6-13*01 100.00 6 0 0 0 297 302 8 13 45 12.4 GCAGCA GCAGCA 6
D SRR765688.30659 IGHD1-14*01 100.00 5 0 0 0 286 290 6 10 181 10.4 AACCG AACCG 5
D SRR765688.30659 IGHD2-15*01 100.00 5 0 0 0 293 297 5 9 181 10.4 TATTG TATTG 5
J SRR765688.30659 IGHJ4*02 93.94 33 2 0 0 311 343 14 46 2e-10 50.1 CTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 13GA8GA10
J SRR765688.30659 IGHJ5*02 93.94 33 2 0 0 311 343 17 49 2e-10 50.1 CTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 13GA8GA10
J SRR765688.30659 IGHJ1*01 90.91 33 3 0 0 311 343 18 50 4e-08 42.1 CTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTC 12ACGA8GA10
# IGBLASTN 2.2.29+
# Query: SRR765688.38189 HQP9GKT01B1F8D length=357|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD3-16*01,IGHD3-16*02,IGHD3-3*01 IGHJ4*02 VH No Out-of-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TGCAA AAACATCCT TTTGGG ACGCGGGCA TGACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 62 30 22 8 0 73.3
FR2-IMGT 63 113 51 49 2 0 96.1
CDR2-IMGT 114 140 27 22 5 0 81.5
FR3-IMGT 141 254 114 103 11 0 90.4
CDR3-IMGT (germline) 255 258 4 4 0 0 100
Total N/A N/A 239 212 27 0 88.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.38189 IGHV6-1*01 88.70 239 27 0 0 20 258 63 301 1e-80 290 CTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAA 5AC12GC3AG6AG2CA2AG2TC2GCCT4CA27TC22TA10GA5AT2TA1CT9CGCTCA3CG3GA9TA14TC7GA23GA27AG5TC7
V SRR765688.38189 IGHV6-1*02 88.70 239 27 0 0 20 258 63 301 1e-80 290 CTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAA 5AC12GC3AG6AG2CA2AG2TC2GCCT4CA27TC22TA10GA5AT2TA1CT9CGCTCA3CG3GA9TA14TC7GA23GA27AG5TC7
V SRR765688.38189 IGHV4-34*12 71.94 196 47 3 8 62 256 99 287 2e-28 117 CTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCC-TCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGC CTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGG--GGAAATCATTCAT-----AGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGC 5CG7AC1GC3TC5TGCG2GAAG2TG2TG6CA1GT2A-A-3CA2TCCA1AT2GTG-T-C-C-G-7TGTC2GC1TATCATTAGCCA4-G2TC2GC1GAAG6AG1TC5CAATACCACGCT3TC2AG4GA16CA16TCCG3AC12AG5TC5
D SRR765688.38189 IGHD3-16*01 100.00 6 0 0 0 268 273 15 20 42 12.4 TTTGGG TTTGGG 6
D SRR765688.38189 IGHD3-16*02 100.00 6 0 0 0 268 273 15 20 42 12.4 TTTGGG TTTGGG 6
D SRR765688.38189 IGHD3-3*01 100.00 6 0 0 0 267 272 11 16 42 12.4 TTTTGG TTTTGG 6
J SRR765688.38189 IGHJ4*02 97.56 41 1 0 0 283 323 8 48 1e-17 73.8 TGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 6TC34
J SRR765688.38189 IGHJ5*02 100.00 34 0 0 0 290 323 18 51 7e-16 67.9 TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 34
J SRR765688.38189 IGHJ4*01 95.12 41 2 0 0 283 323 8 48 3e-15 65.9 TGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 6TC8GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.23676 HQP9GKT01CBCVC length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TTCGA CCCTCCAACCGC GTATAGCAGCA CCTGAGTT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 23 62 40 39 1 0 97.5
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 21 3 0 87.5
FR3-IMGT 162 275 114 103 11 0 90.4
CDR3-IMGT (germline) 276 279 4 3 1 0 75
Total N/A N/A 257 236 21 0 91.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.23676 IGHV7-4-1*02 91.83 257 21 0 0 23 279 36 292 1e-94 336 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC41ACGA8AG14AG1AG9TG3
V SRR765688.23676 IGHV7-4-1*01 91.44 257 22 0 0 23 279 36 292 1e-93 333 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC39AT1ACGA8AG14AG1AG9TG3
V SRR765688.23676 IGHV7-4-1*04 91.44 257 22 0 0 23 279 36 292 1e-93 333 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC22CT18ACGA8AG14AG1AG9TG3
D SRR765688.23676 IGHD6-13*01 100.00 11 0 0 0 292 302 3 13 0.045 22.4 GTATAGCAGCA GTATAGCAGCA 11
D SRR765688.23676 IGHD6-25*01 100.00 10 0 0 0 292 301 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
D SRR765688.23676 IGHD6-6*01 100.00 10 0 0 0 292 301 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
J SRR765688.23676 IGHJ4*02 100.00 35 0 0 0 311 345 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.23676 IGHJ5*02 100.00 35 0 0 0 311 345 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.23676 IGHJ1*01 97.14 35 1 0 0 311 345 18 52 5e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.44566 HQP9GKT01BUA3B length=393|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GATATT CCTACGACCTCGATGGCAATTATGGTGG CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 24 2 0 92.3
CDR1-IMGT 47 70 24 16 8 0 66.7
FR2-IMGT 71 121 51 44 7 0 86.3
CDR2-IMGT 122 142 21 12 9 0 57.1
FR3-IMGT 143 267 125 97 17 11 77.6
CDR3-IMGT (germline) 268 274 7 7 0 0 100
Total N/A N/A 254 200 43 11 78.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.44566 IGHV4-59*02 78.74 254 43 1 11 21 274 50 292 2e-60 223 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.44566 IGHV4-59*01 78.35 254 44 1 11 21 274 50 292 2e-59 219 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.44566 IGHV4-59*07 78.26 253 44 1 11 21 273 50 291 5e-59 218 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA10
D SRR765688.44566 IGHD2-15*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.44566 IGHD2-2*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.44566 IGHD2-2*02 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
J SRR765688.44566 IGHJ4*02 90.91 44 3 1 1 309 351 5 48 7e-10 48.1 CTTTGACTACTGGG-CCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 14-G8TC2GT10GT6
J SRR765688.44566 IGHJ5*02 90.62 32 3 0 0 320 351 20 51 2e-07 40.1 GGGCCAGGGAATCCGGGTCACCGTCGCCTCAG GGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 11TC2GT10GT6
J SRR765688.44566 IGHJ4*01 88.64 44 4 1 1 309 351 5 48 2e-07 40.1 CTTTGACTACTGGG-CCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14-G3GA4TC2GT10GT6
# IGBLASTN 2.2.29+
# Query: SRR765688.49541 HQP9GKT01ESY9C length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GAGTCTCGGCC ACTGGA N/A TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 39 3 0 92.9
CDR1-IMGT 63 86 24 21 3 0 87.5
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 22 2 0 91.7
FR3-IMGT 162 275 114 98 16 0 86
CDR3-IMGT (germline) 276 283 8 6 2 0 75
Total N/A N/A 263 235 28 0 89.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.49541 IGHV7-4-1*02 89.35 263 28 0 0 21 283 34 296 7e-91 324 AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG23AG1CT9TA6GC2AG21GA32GA11TC2TC14AG10CG12AG3AG8CT16TC3AG8GC6AG2AG1TC1GA8AG2TC6AG1AG10GCAG5
V SRR765688.49541 IGHV7-4-1*04 88.97 263 29 0 0 21 283 34 296 6e-90 321 AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG23AG1CT9TA6GC2AG21GA32GA11TC2TC14AG10CG12AG3AG8CT16TC1CT1AG8GC6AG2AG1TC1GA8AG2TC6AG1AG10GCAG5
V SRR765688.49541 IGHV7-4-1*01 88.89 261 29 0 0 21 281 34 294 5e-89 318 AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 12AG23AG1CT9TA6GC2AG21GA32GA11TC2TC14AG10CG12AG3AG8CT16TC3AG8GC5ATAG2AG1TC1GA8AG2TC6AG1AG10GCAG3
D SRR765688.49541 IGHD1-1*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.49541 IGHD1-20*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.49541 IGHD1-7*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
J SRR765688.49541 IGHJ4*02 95.24 42 2 0 0 301 342 7 48 7e-16 67.9 TTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 7TC21GA12
J SRR765688.49541 IGHJ5*02 97.06 34 1 0 0 309 342 18 51 2e-13 60.0 TGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21GA12
J SRR765688.49541 IGHJ4*01 92.86 42 3 0 0 301 342 7 48 2e-13 60.0 TTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 7TC8GA12GA12
# IGBLASTN 2.2.29+
# Query: SRR765688.45233 HQP9GKT01BVKNW length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01,IGHV4-59*07 IGHD7-27*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TGCGA AGAA TGGGGA CACCACCTCGCCCGGATTTAGG TACTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 21 3 0 87.5
FR2-IMGT 71 121 51 50 1 0 98
CDR2-IMGT 122 142 21 19 2 0 90.5
FR3-IMGT 143 256 114 102 12 0 89.5
CDR3-IMGT (germline) 257 260 4 4 0 0 100
Total N/A N/A 240 222 18 0 92.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.45233 IGHV4-59*01 92.50 240 18 0 0 21 260 50 289 2e-89 319 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA 32CT8GA2CT52AG13AG5CG5CA2CATC14CG14GA2CA12CA4TC14CGGC2GA1GC40
V SRR765688.45233 IGHV4-59*07 92.50 240 18 0 0 21 260 50 289 2e-89 319 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA 32CT8GA2CT52AG13AG5CG5CA2CATC14CG14GA2CA12CA4TC14CGGC2GA1GC40
V SRR765688.45233 IGHV4-59*02 92.08 240 19 0 0 21 260 50 289 1e-88 316 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGA 32CT2AG5GA2CT52AG13AG5CG5CA2CATC14CG14GA2CA12CA4TC14CGGC2GA1GC40
D SRR765688.45233 IGHD7-27*01 100.00 6 0 0 0 265 270 6 11 43 12.4 TGGGGA TGGGGA 6
D SRR765688.45233 IGHD2/OR15-2a*01 100.00 5 0 0 0 261 265 1 5 172 10.4 AGAAT AGAAT 5
D SRR765688.45233 IGHD2/OR15-2b*01 100.00 5 0 0 0 261 265 1 5 172 10.4 AGAAT AGAAT 5
J SRR765688.45233 IGHJ4*02 100.00 35 0 0 0 293 327 12 46 2e-16 69.9 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 35
J SRR765688.45233 IGHJ5*02 100.00 33 0 0 0 295 327 17 49 3e-15 65.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 33
J SRR765688.45233 IGHJ4*01 97.14 35 1 0 0 293 327 12 46 4e-14 61.9 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC TACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTC 11GA23
# IGBLASTN 2.2.29+
# Query: SRR765688.17591 HQP9GKT01CLJHN length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-30-4*01 IGHD2-8*01 IGHJ6*02,IGHJ6*04 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TGG TTGTACTAA GGGTTTATGCAGAACGAGGTCGGC CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 19 54 36 35 1 0 97.2
CDR1-IMGT 55 84 30 28 2 0 93.3
FR2-IMGT 85 135 51 50 1 0 98
CDR2-IMGT 136 156 21 20 1 0 95.2
FR3-IMGT 157 270 114 105 9 0 92.1
CDR3-IMGT (germline) 271 278 8 8 0 0 100
Total N/A N/A 260 246 14 0 94.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.17591 IGHV4-30-4*01 94.62 260 14 0 0 19 278 40 299 1e-102 363 CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGA CCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCAGACACGGCCGTGTATTACTGTGCCAGAGA 6GC45CG3GT27TC38AT43CT1TC3GA11AG4GA37AG5GA5AG1AG17
V SRR765688.17591 IGHV4-30-4*02 93.85 260 16 0 0 19 278 40 299 9e-101 357 CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGA CCTTCGGACACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCAGCAGACACGGCCGTGTATTACTGTGCCAGAGA 5AG2GC43CG3GT27TC38AT43CT1TC3GA11AG4GA36CAAG5GA5AG1AG17
V SRR765688.17591 IGHV4-30-4*03 94.02 251 15 0 0 19 269 40 290 2e-97 346 CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTG CCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTG 6GC45CG3GT27TC38AT43CT1TC3GA11AG4GA37AG1AG3GA5AG1AG8
D SRR765688.17591 IGHD2-8*01 100.00 9 0 0 0 282 290 7 15 0.69 18.4 TTGTACTAA TTGTACTAA 9
D SRR765688.17591 IGHD5-24*01 100.00 8 0 0 0 274 281 3 10 2.8 16.4 AGAGATGG AGAGATGG 8
D SRR765688.17591 IGHD2-8*02 100.00 7 0 0 0 282 288 7 13 11 14.4 TTGTACT TTGTACT 7
J SRR765688.17591 IGHJ6*02 92.86 28 2 0 0 315 342 8 35 2e-07 40.1 CTACTACTACGGTGCGGACGTCTGGGGC CTACTACTACGGTATGGACGTCTGGGGC 13GACT13
J SRR765688.17591 IGHJ6*04 92.86 28 2 0 0 315 342 8 35 2e-07 40.1 CTACTACTACGGTGCGGACGTCTGGGGC CTACTACTACGGTATGGACGTCTGGGGC 13GACT13
J SRR765688.17591 IGHJ6*01 92.59 27 2 0 0 315 341 8 34 6e-07 38.2 CTACTACTACGGTGCGGACGTCTGGGG CTACTACTACGGTATGGACGTCTGGGG 13GACT12
# IGBLASTN 2.2.29+
# Query: SRR765688.43976 HQP9GKT01CAKGO length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=14
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-39*01 IGHD1-7*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA ATGGGATGATACTG GGAACTAC TACGCCCGGGCTC ACTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 76 30 28 2 0 93.3
FR2-IMGT 77 127 51 50 1 0 98
CDR2-IMGT 128 145 21 17 1 3 81
FR3-IMGT 146 259 114 108 6 0 94.7
CDR3-IMGT (germline) 260 267 8 8 0 0 100
Total N/A N/A 250 236 11 3 94.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.43976 IGHV4-39*01 94.40 250 11 1 3 21 267 50 299 5e-96 341 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGACA 17GA12AG20GA50CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17
V SRR765688.43976 IGHV4-39*02 93.95 248 12 1 3 21 265 50 297 4e-94 335 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCACTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGA 17GA12AG20GA50CG10-T-A-T8TC8TC29GA11CG11GC11CG5GC26AG15
V SRR765688.43976 IGHV4-39*05 93.88 245 12 1 3 21 262 50 294 9e-93 330 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCG CCCCGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCG 3TC13GA12AG20GA50CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG12
D SRR765688.43976 IGHD1-7*01 100.00 8 0 0 0 282 289 10 17 2.9 16.4 GGAACTAC GGAACTAC 8
D SRR765688.43976 IGHD1-26*01 92.31 13 1 0 0 280 292 8 20 2.9 16.4 TGGGAACTACTAC TGGGAGCTACTAC 5AG7
D SRR765688.43976 IGHD2-8*02 100.00 6 0 0 0 277 282 10 15 46 12.4 TACTGG TACTGG 6
J SRR765688.43976 IGHJ4*02 95.56 45 2 0 0 303 347 4 48 1e-17 73.8 ACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 9CATC34
J SRR765688.43976 IGHJ5*02 100.00 34 0 0 0 314 347 18 51 7e-16 67.9 TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 34
J SRR765688.43976 IGHJ4*01 93.33 45 3 0 0 303 347 4 48 3e-15 65.9 ACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 9CATC8GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.49474 HQP9GKT01DTUWB length=384|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-39*01 IGHD1-7*01 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA ATGGGATGATACTG GGAACTAC TACGCCCGGGCTC ACTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 76 30 28 2 0 93.3
FR2-IMGT 77 128 52 50 1 1 96.2
CDR2-IMGT 129 146 21 17 1 3 81
FR3-IMGT 147 260 114 108 6 0 94.7
CDR3-IMGT (germline) 261 268 8 8 0 0 100
Total N/A N/A 251 236 11 4 94
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.49474 IGHV4-39*01 94.02 251 11 2 4 21 268 50 299 1e-93 333 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCA-GCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGACA 17GA12AG20GA21C-29CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17
V SRR765688.49474 IGHV4-39*02 93.57 249 12 2 4 21 266 50 297 8e-92 327 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCA-GCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCACTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGA 17GA12AG20GA21C-29CG10-T-A-T8TC8TC29GA11CG11GC11CG5GC26AG15
V SRR765688.49474 IGHV4-39*05 93.50 246 12 2 4 21 263 50 294 2e-90 322 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCG CCCCGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCA-GCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCG 3TC13GA12AG20GA21C-29CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG12
D SRR765688.49474 IGHD1-7*01 100.00 8 0 0 0 283 290 10 17 2.9 16.4 GGAACTAC GGAACTAC 8
D SRR765688.49474 IGHD1-26*01 92.31 13 1 0 0 281 293 8 20 2.9 16.4 TGGGAACTACTAC TGGGAGCTACTAC 5AG7
D SRR765688.49474 IGHD2-8*02 100.00 6 0 0 0 278 283 10 15 46 12.4 TACTGG TACTGG 6
J SRR765688.49474 IGHJ4*02 95.56 45 2 0 0 304 348 4 48 1e-17 73.8 ACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 9CATC34
J SRR765688.49474 IGHJ5*02 100.00 34 0 0 0 315 348 18 51 7e-16 67.9 TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 34
J SRR765688.49474 IGHJ4*01 93.33 45 3 0 0 304 348 4 48 3e-15 65.9 ACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 9CATC8GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.34997 HQP9GKT01BF6LJ length=395|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-13*01,IGHD6-6*01 IGHJ5*01,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG CTTCCTGTAGCATT AGCAGCTG CCATGCCCAGCA GTTCG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 38 4 0 90.5
CDR1-IMGT 63 86 24 17 7 0 70.8
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 101 13 0 88.6
CDR3-IMGT (germline) 276 282 7 7 0 0 100
Total N/A N/A 262 232 30 0 88.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.34997 IGHV7-4-1*02 88.55 262 30 0 0 21 282 34 295 2e-88 316 AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 18AG2GA1AGAG24TCTC3CAGC1GAAGTC8AG11CA45GA1GC5AG2TC19AT27AC10CG6TA1TA4AC6AGTC1AG7AG1GT2AG16TA11
V SRR765688.34997 IGHV7-4-1*04 88.17 262 31 0 0 21 282 34 295 1e-87 313 AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 18AG2GA1AGAG24TCTC3CAGC1GAAGTC8AG11CA45GA1GC5AG2TC19AT27AC10CG2CT3TA1TA4AC6AGTC1AG7AG1GT2AG16TA11
V SRR765688.34997 IGHV7-4-1*01 88.12 261 31 0 0 21 281 34 294 4e-87 311 AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 18AG2GA1AGAG24TCTC3CAGC1GAAGTC8AG11CA45GA1GC5AG2TC19AT27AC10CG6TA1TA4AC5ATAGTC1AG7AG1GT2AG16TA10
D SRR765688.34997 IGHD6-13*01 100.00 8 0 0 0 297 304 10 17 3.0 16.4 AGCAGCTG AGCAGCTG 8
D SRR765688.34997 IGHD6-6*01 100.00 8 0 0 0 296 303 6 13 3.0 16.4 TAGCAGCT TAGCAGCT 8
D SRR765688.34997 IGHD2-2*01 92.31 13 1 0 0 299 311 19 31 3.0 16.4 CAGCTGCCATGCC CAGCTGCTATGCC 7CT5
J SRR765688.34997 IGHJ5*01 93.02 43 3 0 0 317 359 8 50 5e-14 61.9 GTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA GTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCA 18GA7GT5TC10
J SRR765688.34997 IGHJ5*02 93.02 43 3 0 0 317 359 8 50 5e-14 61.9 GTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA GTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 7TC18GT5TC10
J SRR765688.34997 IGHJ4*02 92.31 39 3 0 0 321 359 9 47 1e-11 54.0 GACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 4CA17GT5TC10
# IGBLASTN 2.2.29+
# Query: SRR765688.33811 HQP9GKT01AZ9Y4 length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD3-9*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GGGGTAT TATTTTGA TACCACTGGTTCTCCGAGGTCAC ACGGT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 47 4 0 92.2
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 103 11 0 90.4
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 237 26 0 90.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.33811 IGHV7-4-1*02 90.11 263 26 0 0 21 283 34 296 1e-92 330 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 26CT16CG5GCTC6GAAGGC6CA3CA24GA6CG23GAGAAC6GA18CG12TG11GC1CT21TA8CG6GA1TC6CG16TG5TC11
V SRR765688.33811 IGHV7-4-1*04 89.73 263 27 0 0 21 283 34 296 8e-92 327 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 26CT16CG5GCTC6GAAGGC6CA3CA24GA6CG23GAGAAC6GA18CG12TG11GC1CT17CT3TA8CG6GA1TC6CG16TG5TC11
V SRR765688.33811 IGHV7-4-1*01 89.66 261 27 0 0 21 281 34 294 7e-91 324 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 26CT16CG5GCTC6GAAGGC6CA3CA24GA6CG23GAGAAC6GA18CG12TG11GC1CT21TA8CG3AT2GA1TC6CG16TG5TC9
D SRR765688.33811 IGHD3-9*01 100.00 8 0 0 0 291 298 10 17 3.0 16.4 TATTTTGA TATTTTGA 8
D SRR765688.33811 IGHD3-16*01 100.00 7 0 0 0 287 293 1 7 12 14.4 GTATTAT GTATTAT 7
D SRR765688.33811 IGHD3-16*02 100.00 7 0 0 0 287 293 1 7 12 14.4 GTATTAT GTATTAT 7
J SRR765688.33811 IGHJ6*02 95.74 47 2 0 0 322 368 16 62 8e-19 77.8 ACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA ACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 5CA23GA17
J SRR765688.33811 IGHJ6*01 93.75 48 3 0 0 322 369 16 63 5e-17 71.9 ACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAG ACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 5CA13CG9GA18
J SRR765688.33811 IGHJ6*04 93.75 48 3 0 0 322 369 16 63 5e-17 71.9 ACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAG ACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 5CA14CA8GA18
# IGBLASTN 2.2.29+
# Query: SRR765688.40396 HQP9GKT01DTRVH length=361|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01,IGHV4-59*02,IGHV4-59*07 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG G GGATATT CCTATGATCTCTATGGCAATAAAGGAGGT TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 56 24 12 12 0 50
FR2-IMGT 57 107 51 41 10 0 80.4
CDR2-IMGT 108 128 21 11 10 0 52.4
FR3-IMGT 129 242 114 98 16 0 86
CDR3-IMGT (germline) 243 247 5 5 0 0 100
Total N/A N/A 228 179 49 0 78.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.40396 IGHV4-59*01 78.51 228 49 0 0 20 247 63 290 8e-55 204 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12TC11AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
V SRR765688.40396 IGHV4-59*02 78.51 228 49 0 0 20 247 63 290 8e-55 204 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TGCT1GA1CTCAAGCT2TC2TC4CGTC12TC11AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
V SRR765688.40396 IGHV4-59*07 78.51 228 49 0 0 20 247 63 290 8e-55 204 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12TC11AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
D SRR765688.40396 IGHD2-15*01 100.00 7 0 0 0 249 255 2 8 11 14.4 GGATATT GGATATT 7
D SRR765688.40396 IGHD2-2*01 100.00 7 0 0 0 249 255 2 8 11 14.4 GGATATT GGATATT 7
D SRR765688.40396 IGHD2-2*02 100.00 7 0 0 0 249 255 2 8 11 14.4 GGATATT GGATATT 7
J SRR765688.40396 IGHJ4*02 93.02 43 3 0 0 285 327 6 48 4e-14 61.9 TTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 8TC13TC2GT17
J SRR765688.40396 IGHJ4*01 90.70 43 4 0 0 285 327 6 48 1e-11 54.0 TTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 8TC8GA4TC2GT17
J SRR765688.40396 IGHJ5*02 94.12 34 2 0 0 294 327 18 51 4e-11 52.0 TGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 13TC2GT17
# IGBLASTN 2.2.29+
# Query: SRR765688.38176 HQP9GKT01CL3CG length=383|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GATATT CCTATAATCTGGATGGCAATTATGGTGGT TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 23 3 0 88.5
CDR1-IMGT 47 70 24 15 9 0 62.5
FR2-IMGT 71 121 51 46 5 0 90.2
CDR2-IMGT 122 142 21 16 5 0 76.2
FR3-IMGT 143 256 114 97 17 0 85.1
CDR3-IMGT (germline) 257 263 7 7 0 0 100
Total N/A N/A 243 204 39 0 84
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.38176 IGHV4-59*01 83.95 243 39 0 0 21 263 50 292 3e-71 258 CCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 7TC10GC1AG7CT1CG5CT1GACGCTCAAG6TC4AGTC12TC28TG1TA7GT1ACGA5GATG6GC23CA8GA8TCTA6GA1GA2AC4GC7GAAG4CG3CT8CT14CG2CT1TA11
V SRR765688.38176 IGHV4-59*07 83.88 242 39 0 0 21 262 50 291 1e-70 257 CCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 7TC10GC1AG7CT1CG5CT1GACGCTCAAG6TC4AGTC12TC28TG1TA7GT1ACGA5GATG6GC23CA8GA8TCTA6GA1GA2AC4GC7GAAG4CG3CT8CT14CG2CT1TA10
V SRR765688.38176 IGHV4-59*08 83.88 242 39 0 0 21 262 50 291 1e-70 257 CCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGA 7TC10GC1AG7CT1CG5CT1GACGCTCAAG6TC4AGTC12TC28TG1TA7GT1ACGA5GATG6GC23CA8GA8TCTA6GA1GA2AC4GC7GAAG4CG3CT11GA11CG2CT1TA10
D SRR765688.38176 IGHD2-15*01 100.00 8 0 0 0 262 269 1 8 2.8 16.4 AGGATATT AGGATATT 8
D SRR765688.38176 IGHD2-2*01 100.00 8 0 0 0 262 269 1 8 2.8 16.4 AGGATATT AGGATATT 8
D SRR765688.38176 IGHD2-2*02 100.00 8 0 0 0 262 269 1 8 2.8 16.4 AGGATATT AGGATATT 8
J SRR765688.38176 IGHJ4*02 93.02 43 3 0 0 299 341 6 48 4e-14 61.9 TTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22TC2GT4TA12
J SRR765688.38176 IGHJ4*01 90.70 43 4 0 0 299 341 6 48 1e-11 54.0 TTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 17GA4TC2GT4TA12
J SRR765688.38176 IGHJ5*02 91.43 35 3 0 0 307 341 17 51 3e-09 46.1 CTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 14TC2GT4TA12
# IGBLASTN 2.2.29+
# Query: SRR765688.36681 HQP9GKT01B98GC length=382|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=13
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD3-22*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA AGAACGTCGA AGTAGTGGTTAT (T) TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 47 4 0 92.2
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 105 9 0 92.1
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 241 22 0 91.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.36681 IGHV7-4-1*02 91.63 263 22 0 0 21 283 34 296 2e-96 343 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 34GA13CA6TC2AG6AT2AGCA18GC9TG35AG1GATC2GAGA1CG7GA12GA53TCGA17TA3GC1TG19
V SRR765688.36681 IGHV7-4-1*04 91.25 263 23 0 0 21 283 34 296 1e-95 339 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 34GA13CA6TC2AG6AT2AGCA18GC9TG35AG1GATC2GAGA1CG7GA12GA34CT18TCGA17TA3GC1TG19
V SRR765688.36681 IGHV7-4-1*01 91.19 261 23 0 0 21 281 34 294 1e-94 336 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 34GA13CA6TC2AG6AT2AGCA18GC9TG35AG1GATC2GAGA1CG7GA12GA51AT1TCGA17TA3GC1TG17
D SRR765688.36681 IGHD3-22*01 100.00 13 0 0 0 294 306 14 26 0.003 26.4 AGTAGTGGTTATT AGTAGTGGTTATT 13
D SRR765688.36681 IGHD3-3*01 100.00 10 0 0 0 297 306 17 26 0.18 20.4 AGTGGTTATT AGTGGTTATT 10
D SRR765688.36681 IGHD3-3*02 100.00 10 0 0 0 297 306 17 26 0.18 20.4 AGTGGTTATT AGTGGTTATT 10
J SRR765688.36681 IGHJ4*02 95.35 43 2 0 0 306 348 6 48 2e-16 69.9 TTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 6CT14GA21
J SRR765688.36681 IGHJ5*02 95.00 40 2 0 0 309 348 12 51 1e-14 63.9 GACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG GACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4AC13GA21
J SRR765688.36681 IGHJ4*01 93.02 43 3 0 0 306 348 6 48 5e-14 61.9 TTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 6CT10GA3GA21
# IGBLASTN 2.2.29+
# Query: SRR765688.26204 HQP9GKT01AGSP2 length=365|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD1-1*01,IGHD1-1*01 IGHJ5*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG GGGAAGGG ACAACTG CAACTTACAAC TTCGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 27 3 0 90
FR2-IMGT 63 112 51 50 0 1 98
CDR2-IMGT 113 139 27 24 3 0 88.9
FR3-IMGT 140 253 114 105 9 0 92.1
CDR3-IMGT (germline) 254 260 7 7 0 0 100
Total N/A N/A 242 226 15 1 93.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.26204 IGHV6-1*01 93.39 242 15 1 1 20 260 63 304 2e-90 322 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 35CG2ACAT49-G8TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24
V SRR765688.26204 IGHV6-1*02 93.39 242 15 1 1 20 260 63 304 2e-90 322 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 35CG2ACAT49-G8TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24
V SRR765688.26204 IGHV4-4*08 68.02 222 64 2 7 40 260 77 292 1e-27 114 GTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATTATACAGGTCCAAGTGGTATAAC-AATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG GTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGG------TATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGAG 3TG3TC1GT2AGCT1CG1GT1ACGTCATC4AG7AC5TC5TGCG2GAAG2CA2TG6CA1GT1AGAGG-G-A-C-A-T-4CTACGTGA1CA1AC5TG1TGAC2-C2TC2TCGACAACGCTCAC2TCGC1GC2AG2CT3AG1AC5CAATAC2CT5TCAG1AC19CA7GC8TCCG3ACCA3GA4TC14AG4
D SRR765688.26204 IGHD1-1*01 100.00 7 0 0 0 269 275 4 10 11 14.4 ACAACTG ACAACTG 7
D SRR765688.26204 IGHD1-1*01 100.00 7 0 0 0 281 287 3 9 11 14.4 TACAACT TACAACT 7
D SRR765688.26204 IGHD1-20*01 100.00 5 0 0 0 271 275 6 10 173 10.4 AACTG AACTG 5
J SRR765688.26204 IGHJ5*02 100.00 43 0 0 0 287 329 9 51 3e-21 85.7 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 43
J SRR765688.26204 IGHJ4*02 100.00 35 0 0 0 295 329 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.26204 IGHJ5*01 95.35 43 2 0 0 287 329 9 51 2e-16 69.9 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 6CT10GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.31319 HQP9GKT01B1IO7 length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG (A) CTGGA CGGGACCGACTCG GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 22 62 41 40 1 0 97.6
CDR1-IMGT 63 86 24 19 5 0 79.2
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 19 5 0 79.2
FR3-IMGT 162 275 114 102 12 0 89.5
CDR3-IMGT (germline) 276 281 6 5 1 0 83.3
Total N/A N/A 260 235 25 0 90.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.31319 IGHV7-4-1*02 90.38 260 25 0 0 22 281 35 294 3e-92 329 AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGA AGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 23AG24TC7CA1TC3TG1CT2AG51GA6GA3CT4CAGC5AG10AG12AG20GC8TC11AG7AGTC1AG7TG4CG11CG10TC4
V SRR765688.31319 IGHV7-4-1*01 90.00 260 26 0 0 22 281 35 294 2e-91 325 AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGA AGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 23AG24TC7CA1TC3TG1CT2AG51GA6GA3CT4CAGC5AG10AG12AG20GC8TC11AG6ATAGTC1AG7TG4CG11CG10TC4
V SRR765688.31319 IGHV7-4-1*04 90.00 260 26 0 0 22 281 35 294 2e-91 325 AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGA AGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 23AG24TC7CA1TC3TG1CT2AG51GA6GA3CT4CAGC5AG10AG12AG20GC8TC1CT9AG7AGTC1AG7TG4CG11CG10TC4
D SRR765688.31319 IGHD1-1*01 100.00 6 0 0 0 281 286 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.31319 IGHD1-20*01 100.00 6 0 0 0 281 286 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.31319 IGHD1-7*01 100.00 6 0 0 0 281 286 7 12 45 12.4 ACTGGA ACTGGA 6
J SRR765688.31319 IGHJ4*02 100.00 40 0 0 0 300 339 9 48 2e-19 79.8 GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 40
J SRR765688.31319 IGHJ4*01 97.50 40 1 0 0 300 339 9 48 5e-17 71.9 GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14GA25
J SRR765688.31319 IGHJ5*02 100.00 35 0 0 0 305 339 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.20699 HQP9GKT01DRRB0 length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV3-30-3*01 IGHD5-24*01 IGHJ4*02 VH No Out-of-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTTCG (AGA) GATGG ACCCAACTCCCCTC GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 19 73 55 52 3 0 94.5
CDR1-IMGT 74 97 24 21 3 0 87.5
FR2-IMGT 98 148 51 47 4 0 92.2
CDR2-IMGT 149 172 24 20 4 0 83.3
FR3-IMGT 173 286 114 102 12 0 89.5
CDR3-IMGT (germline) 287 292 6 5 1 0 83.3
Total N/A N/A 274 247 27 0 90.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.20699 IGHV3-30-3*01 90.15 274 27 0 0 19 292 21 294 6e-97 344 TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGA TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGCAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGA 47TC2TCTC10AC7CACG16CG22TG3CG11AG2TA1GA4CA10GA7TC2TC5AC47CG6TC1AG18CG3TA6AG1GC3AG5TC3TG5
V SRR765688.20699 IGHV3-30*04 89.86 276 28 0 0 19 294 21 296 6e-97 344 TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGA TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGA 47TC2TCTC10AC7CACG16CG22TG3CG11AG2TA1GA4CA9CTGA7TC2TC5AC47CG6TC1AG18CG3TA6AG1GC3AG5TC3TG7
V SRR765688.20699 IGHV3-30*14 89.86 276 28 0 0 19 294 21 296 6e-97 344 TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGA TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTTCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGA 47TC2TCTC10AC7CACG16CG22TG3CG11AG2TA1GA4CA9CTGA7TC2TC5AC47CG6TC1AT18CG3TA6AG1GC3AG5TC3TG7
D SRR765688.20699 IGHD5-24*01 100.00 8 0 0 0 290 297 3 10 2.9 16.4 AGAGATGG AGAGATGG 8
D SRR765688.20699 IGHD4-23*01 100.00 6 0 0 0 302 307 14 19 46 12.4 AACTCC AACTCC 6
D SRR765688.20699 IGHD1-1*01 100.00 5 0 0 0 301 305 5 9 184 10.4 CAACT CAACT 5
J SRR765688.20699 IGHJ4*02 96.97 33 1 0 0 312 344 9 41 7e-13 58.0 GACTACTGGGGCCTGGGAACCCTGGTCACCGTC GACTACTGGGGCCAGGGAACCCTGGTCACCGTC 13TA19
J SRR765688.20699 IGHJ4*01 93.94 33 2 0 0 312 344 9 41 2e-10 50.1 GACTACTGGGGCCTGGGAACCCTGGTCACCGTC GACTACTGGGGCCAAGGAACCCTGGTCACCGTC 13TAGA18
J SRR765688.20699 IGHJ5*02 96.43 28 1 0 0 317 344 17 44 7e-10 48.1 CTGGGGCCTGGGAACCCTGGTCACCGTC CTGGGGCCAGGGAACCCTGGTCACCGTC 8TA19
# IGBLASTN 2.2.29+
# Query: SRR765688.48796 HQP9GKT01B94FW length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR11|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV3-33*01 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GGATGATACCAGTACCTGGTACGGGCGACTCCG GTACTACT ATGCC ATGGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
CDR1-IMGT 19 36 18 17 1 0 94.4
FR2-IMGT 37 87 51 51 0 0 100
CDR2-IMGT 88 111 24 23 1 0 95.8
FR3-IMGT 112 225 114 106 8 0 93
CDR3-IMGT (germline) 226 233 8 8 0 0 100
Total N/A N/A 215 205 10 0 95.3
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.48796 IGHV3-33*01 95.35 215 10 0 0 19 233 82 296 3e-85 305 ACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGA ACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAGA 3AT86GA48CA3ACCA8GC11CA4TC26TG5TC11
V SRR765688.48796 IGHV3-33*06 94.88 215 11 0 0 19 233 82 296 2e-84 302 ACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGA ACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAAAGA 3AT86GA48CA3ACCA8GC11CA4TC26TG5TC7GA3
V SRR765688.48796 IGHV3-33*03 94.42 215 12 0 0 19 233 82 296 2e-83 299 ACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGA ACCTTCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAAAGA 3AT86GA48CATC2ACCA8GC11CA4TC26TG5TC7GA3
D SRR765688.48796 IGHD2/OR15-2a*01 100.00 8 0 0 0 267 274 15 22 2.7 16.4 GTACTACT GTACTACT 8
D SRR765688.48796 IGHD2/OR15-2b*01 100.00 8 0 0 0 267 274 15 22 2.7 16.4 GTACTACT GTACTACT 8
D SRR765688.48796 IGHD3-10*01 100.00 7 0 0 0 271 277 5 11 11 14.4 TACTATG TACTATG 7
J SRR765688.48796 IGHJ6*02 97.62 42 1 0 0 280 321 21 62 3e-18 75.8 ATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCA ATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 35GC6
J SRR765688.48796 IGHJ6*01 95.35 43 2 0 0 280 322 21 63 2e-16 69.9 ATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCAG ATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 14CG20GC7
J SRR765688.48796 IGHJ6*03 95.35 43 2 0 0 279 321 20 62 2e-16 69.9 CATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCA CATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCA 16CA19GC6
# IGBLASTN 2.2.29+
# Query: SRR765688.41848 HQP9GKT01C3DX5 length=387|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD2-15*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCCGAACGCGA TAGTGGTGG AAG ACTGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 40 2 0 95.2
CDR1-IMGT 63 86 24 22 2 0 91.7
FR2-IMGT 87 137 51 51 0 0 100
CDR2-IMGT 138 161 24 23 1 0 95.8
FR3-IMGT 162 275 114 106 8 0 93
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 250 13 0 95.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.41848 IGHV7-4-1*02 95.06 263 13 0 0 21 283 34 296 6e-105 371 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 24AG13CT9CA10TC68GC14AG4TC5AG25CT29GC14GA4CT19TA12
V SRR765688.41848 IGHV7-4-1*04 94.68 263 14 0 0 21 283 34 296 5e-104 367 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 24AG13CT9CA10TC68GC14AG4TC5AG25CT18CT10GC14GA4CT19TA12
V SRR765688.41848 IGHV7-4-1*01 94.64 261 14 0 0 21 281 34 294 5e-103 364 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 24AG13CT9CA10TC68GC14AG4TC5AG25CT29GC5AT8GA4CT19TA10
D SRR765688.41848 IGHD2-15*01 100.00 9 0 0 0 295 303 10 18 0.74 18.4 TAGTGGTGG TAGTGGTGG 9
D SRR765688.41848 IGHD1-26*01 100.00 7 0 0 0 294 300 4 10 12 14.4 ATAGTGG ATAGTGG 7
D SRR765688.41848 IGHD2-21*01 100.00 7 0 0 0 297 303 9 15 12 14.4 GTGGTGG GTGGTGG 7
J SRR765688.41848 IGHJ5*02 97.73 44 1 0 0 307 350 4 47 2e-19 79.8 ACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCC ACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCC 31AG12
J SRR765688.41848 IGHJ5*01 93.18 44 3 0 0 307 350 4 47 1e-14 63.9 ACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCC ACTGGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCC 11CT10GA8AG12
J SRR765688.41848 IGHJ4*02 96.77 31 1 0 0 320 350 14 44 1e-11 54.0 CTGGGGCCAGGGAACCCTAGTCACCGTCTCC CTGGGGCCAGGGAACCCTGGTCACCGTCTCC 18AG12
# IGBLASTN 2.2.29+
# Query: SRR765688.23863 HQP9GKT01BPQLA length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD3-10*01,IGHD3-10*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA CAAGAG TCGGG ATG ACTAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 11 2 0 84.6
CDR1-IMGT 33 62 30 25 5 0 83.3
FR2-IMGT 63 113 51 51 0 0 100
CDR2-IMGT 114 140 27 27 0 0 100
FR3-IMGT 141 254 114 101 13 0 88.6
CDR3-IMGT (germline) 255 262 8 8 0 0 100
Total N/A N/A 243 223 20 0 91.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 8 hits found
V SRR765688.23863 IGHV6-1*01 91.77 243 20 0 0 20 262 63 305 5e-89 318 CTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 3CT1TC9AG4AGCT9GA3CG94AG1TA3AG2GA12TCTC5GC20CG12TC4GC3CG22CG5TC11
V SRR765688.23863 IGHV6-1*02 91.77 243 20 0 0 20 262 63 305 5e-89 318 CTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 3CT1TC9AG4AGCT9GA3CG94AG1TA3AG2GA12TCTC5GC20CG12TC4GC3CG22CG5TC11
V SRR765688.23863 IGHV4-39*02 65.50 200 63 1 6 63 262 106 299 5e-21 92.2 TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGA TGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTG------GGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCACTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGAGA 3AGAG7AC1GC3TC5TGCG2GAAG2CG2TG6CA1GT2AG2GT1CTAC2CT2CT2GTTGC-C-A-A-G-T-2TAAGTC1ACTCGT1TC2TCGACAACACTCTG2TCAC1GCGA1AG6AG1AC1TCTC2CAATACGCCGCT6AG21CA2TC3AGGC3CG4TCCG3ACGA11CG5TC5AG5
D SRR765688.23863 IGHD3-10*01 100.00 5 0 0 0 269 273 14 18 170 10.4 TCGGG TCGGG 5
D SRR765688.23863 IGHD3-10*02 100.00 5 0 0 0 269 273 13 17 170 10.4 TCGGG TCGGG 5
J SRR765688.23863 IGHJ4*02 95.45 44 2 0 0 277 320 1 44 4e-17 71.9 ACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC ACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCC 6AT4CT32
J SRR765688.23863 IGHJ5*02 97.22 36 1 0 0 285 320 12 47 1e-14 63.9 GACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC GACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCC 4AC31
J SRR765688.23863 IGHJ4*01 93.18 44 3 0 0 277 320 1 44 1e-14 63.9 ACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC ACTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCC 6AT4CT10GA21
# IGBLASTN 2.2.29+
# Query: SRR765688.17127 HQP9GKT01CQJSD length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 N/A IGHJ3*01 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA N/A N/A CAGACATTCATG TGCTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 42 0 0 100
CDR1-IMGT 63 86 24 21 3 0 87.5
FR2-IMGT 87 137 51 48 3 0 94.1
CDR2-IMGT 138 161 24 21 3 0 87.5
FR3-IMGT 162 275 114 107 7 0 93.9
CDR3-IMGT (germline) 276 283 8 7 1 0 87.5
Total N/A N/A 263 246 17 0 93.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 6 hits found
V SRR765688.17127 IGHV7-4-1*02 93.54 263 17 0 0 21 283 34 296 3e-101 358 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 57GACG4TG44CA6AGTG6CA1AC8TG8AG53TC8CTGC10TC6GA19AG9AG7
V SRR765688.17127 IGHV7-4-1*04 93.16 263 18 0 0 21 283 34 296 3e-100 355 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 57GACG4TG44CA6AGTG6CA1AC8TG8AG53TC1CT6CTGC10TC6GA19AG9AG7
V SRR765688.17127 IGHV7-4-1*01 93.10 261 18 0 0 21 281 34 294 2e-99 352 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 57GACG4TG44CA6AGTG6CA1AC8TG8AG53TC8CTGC8AT1TC6GA19AG9AG5
J SRR765688.17127 IGHJ3*01 97.87 47 1 0 0 296 342 4 50 3e-21 85.7 TGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG TGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 12GC34
J SRR765688.17127 IGHJ3*02 95.74 47 2 0 0 296 342 4 50 8e-19 77.8 TGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG TGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 10GA1GC34
J SRR765688.17127 IGHJ4*03 91.18 34 3 0 0 309 342 15 48 1e-08 44.1 TGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG TGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 14ACAC13TC4
# IGBLASTN 2.2.29+
# Query: SRR765688.32432 HQP9GKT01CZ7DE length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*01,IGHV1-69*12,IGHV1-69*13 IGHD6-13*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGTCCCA AGCAGCTG AT TACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 38 18 18 0 0 100
CDR1-IMGT 39 62 24 21 3 0 87.5
FR2-IMGT 63 113 51 51 0 0 100
CDR2-IMGT 114 137 24 23 1 0 95.8
FR3-IMGT 138 251 114 99 15 0 86.8
CDR3-IMGT (germline) 252 258 7 7 0 0 100
Total N/A N/A 238 219 19 0 92
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.32432 IGHV1-69*01 92.02 238 19 0 0 21 258 58 295 1e-87 313 GTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG GTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 26GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.32432 IGHV1-69*12 92.02 238 19 0 0 21 258 58 295 1e-87 313 GTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG GTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 26GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.32432 IGHV1-69*13 92.02 238 19 0 0 21 258 58 295 1e-87 313 GTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG GTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 26GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
D SRR765688.32432 IGHD6-13*01 100.00 8 0 0 0 266 273 10 17 2.7 16.4 AGCAGCTG AGCAGCTG 8
D SRR765688.32432 IGHD3-16*01 100.00 7 0 0 0 272 278 7 13 11 14.4 TGATTAC TGATTAC 7
D SRR765688.32432 IGHD3-16*02 100.00 7 0 0 0 272 278 7 13 11 14.4 TGATTAC TGATTAC 7
J SRR765688.32432 IGHJ6*02 98.04 51 1 0 0 276 326 9 59 1e-23 93.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCC 38AG12
J SRR765688.32432 IGHJ6*01 96.08 51 2 0 0 276 326 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCC 26CG11AG12
J SRR765688.32432 IGHJ6*04 96.08 51 2 0 0 276 326 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCC 27CA10AG12
# IGBLASTN 2.2.29+
# Query: SRR765688.20304 HQP9GKT01AMKZ5 length=386|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD2-21*02 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GGGACTGTGGGACGGCAGGGTT GTGACT (G) ACCTC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 40 2 0 95.2
CDR1-IMGT 63 86 24 17 7 0 70.8
FR2-IMGT 87 137 51 48 3 0 94.1
CDR2-IMGT 138 161 24 19 5 0 79.2
FR3-IMGT 162 275 114 92 22 0 80.7
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 224 39 0 85.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.20304 IGHV7-4-1*02 85.17 263 39 0 0 21 283 34 296 1e-80 290 AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG13CT20TC1GCTC5CT1AGTC1TA23TC12AG9CA13TC5TC1AC3CG2TC5AG7CA4CT10AGAT16ACGA5CG3AG6CA1TA3AG8TC1AG3GACA2AG4AG2TC6AG1TG4TA12
V SRR765688.20304 IGHV7-4-1*04 84.79 263 40 0 0 21 283 34 296 1e-79 286 AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG13CT20TC1GCTC5CT1AGTC1TA23TC12AG9CA13TC5TC1AC3CG2TC5AG7CA4CT10AGAT16ACGA5CG3AG2CT3CA1TA3AG8TC1AG3GACA2AG4AG2TC6AG1TG4TA12
V SRR765688.20304 IGHV7-4-1*01 84.67 261 40 0 0 21 281 34 294 1e-78 283 AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 12AG13CT20TC1GCTC5CT1AGTC1TA23TC12AG9CA13TC5TC1AC3CG2TC5AG7CA4CT10AGAT16ACGA5CG3AG6CA1TA3AG6AT1TC1AG3GACA2AG4AG2TC6AG1TG4TA10
D SRR765688.20304 IGHD2-21*02 100.00 7 0 0 0 306 312 15 21 12 14.4 GTGACTG GTGACTG 7
D SRR765688.20304 IGHD1-26*01 100.00 6 0 0 0 290 295 7 12 46 12.4 GTGGGA GTGGGA 6
D SRR765688.20304 IGHD3-9*01 100.00 6 0 0 0 307 312 15 20 46 12.4 TGACTG TGACTG 6
J SRR765688.20304 IGHJ5*02 97.37 38 1 0 0 312 349 12 49 7e-16 67.9 GACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC GACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 4TC33
J SRR765688.20304 IGHJ4*02 100.00 33 0 0 0 317 349 14 46 3e-15 65.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 33
J SRR765688.20304 IGHJ1*01 96.97 33 1 0 0 317 349 18 50 7e-13 58.0 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTC 12AC20
# IGBLASTN 2.2.29+
# Query: SRR765688.47093 HQP9GKT01BTC88 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCTGCCAGTGGG TGGAACGAC ACCGTC TGACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 39 3 0 92.9
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 22 2 0 91.7
FR3-IMGT 162 275 114 106 8 0 93
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 244 19 0 92.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.47093 IGHV7-4-1*02 92.78 263 19 0 0 21 283 34 296 3e-99 352 AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 22GA10GA7CT14CTTACG4AG4AGCA67AC2GA26AG49AG2AG1GC2CA13CT3AG1TG17
V SRR765688.47093 IGHV7-4-1*04 92.40 263 20 0 0 21 283 34 296 2e-98 349 AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 22GA10GA7CT14CTTACG4AG4AGCA67AC2GA26AG31CT17AG2AG1GC2CA13CT3AG1TG17
V SRR765688.47093 IGHV7-4-1*01 92.34 261 20 0 0 21 281 34 294 2e-97 346 AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 22GA10GA7CT14CTTACG4AG4AGCA67AC2GA26AG48ATAG2AG1GC2CA13CT3AG1TG15
D SRR765688.47093 IGHD1-1*01 100.00 9 0 0 0 296 304 9 17 0.73 18.4 TGGAACGAC TGGAACGAC 9
D SRR765688.47093 IGHD1-20*01 100.00 9 0 0 0 296 304 9 17 0.73 18.4 TGGAACGAC TGGAACGAC 9
D SRR765688.47093 IGHD1-26*01 100.00 6 0 0 0 290 295 6 11 46 12.4 AGTGGG AGTGGG 6
J SRR765688.47093 IGHJ4*02 97.56 41 1 0 0 311 351 8 48 1e-17 73.8 TGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG TGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 24AG16
J SRR765688.47093 IGHJ4*01 95.12 41 2 0 0 311 351 8 48 3e-15 65.9 TGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG TGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 15GA8AG16
J SRR765688.47093 IGHJ5*02 97.14 35 1 0 0 317 351 17 51 5e-14 61.9 CTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 18AG16
# IGBLASTN 2.2.29+
# Query: SRR765688.46353 HQP9GKT01CZULB length=366|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD1-1*01,IGHD1-1*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG GGGAAGGG ACAACTG CAACTTACAAC TTCGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 27 3 0 90
FR2-IMGT 63 113 51 51 0 0 100
CDR2-IMGT 114 140 27 24 3 0 88.9
FR3-IMGT 141 254 114 105 9 0 92.1
CDR3-IMGT (germline) 255 261 7 7 0 0 100
Total N/A N/A 242 227 15 0 93.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46353 IGHV6-1*01 93.80 242 15 0 0 20 261 63 304 3e-93 332 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 35CG2ACAT58TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24
V SRR765688.46353 IGHV6-1*02 93.80 242 15 0 0 20 261 63 304 3e-93 332 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 35CG2ACAT58TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24
V SRR765688.46353 IGHV4-4*08 68.16 223 63 2 8 40 261 77 292 5e-28 115 GTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAAC-AATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAG GTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGG-------TATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGAG 3TG3TC1GT2AGCT1CG1GT1ACGTCATC4AG7AC5TC5TGCG2GAAG2CA2TG6CA1GT2AGA-G-G-A-C-A-T-4CTACGTGA1CA1AC5TG1TGAC2-C2TC2TCGACAACGCTCAC2TCGC1GC2AG2CT3AG1AC5CAATAC2CT5TCAG1AC19CA7GC8TCCG3ACCA3GA4TC14AG4
D SRR765688.46353 IGHD1-1*01 100.00 7 0 0 0 270 276 4 10 11 14.4 ACAACTG ACAACTG 7
D SRR765688.46353 IGHD1-1*01 100.00 7 0 0 0 282 288 3 9 11 14.4 TACAACT TACAACT 7
D SRR765688.46353 IGHD1-20*01 100.00 5 0 0 0 272 276 6 10 173 10.4 AACTG AACTG 5
J SRR765688.46353 IGHJ5*02 100.00 43 0 0 0 288 330 9 51 3e-21 85.7 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 43
J SRR765688.46353 IGHJ4*02 100.00 35 0 0 0 296 330 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.46353 IGHJ5*01 95.35 43 2 0 0 288 330 9 51 2e-16 69.9 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 6CT10GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.49658 HQP9GKT01D85J4 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GATATT CCTACGACCTCGATGGCAATTATGGTGG CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 24 2 0 92.3
CDR1-IMGT 47 70 24 16 8 0 66.7
FR2-IMGT 71 121 51 44 7 0 86.3
CDR2-IMGT 122 142 21 11 10 0 52.4
FR3-IMGT 143 267 125 97 17 11 77.6
CDR3-IMGT (germline) 268 274 7 7 0 0 100
Total N/A N/A 254 199 44 11 78.3
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.49658 IGHV4-59*02 78.35 254 44 1 11 21 274 50 292 2e-59 219 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA1CTTG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.49658 IGHV4-59*01 77.95 254 45 1 11 21 274 50 292 2e-58 216 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA1CTTG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.49658 IGHV4-59*07 77.87 253 45 1 11 21 273 50 291 5e-58 215 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA1CTTG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA10
D SRR765688.49658 IGHD2-15*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.49658 IGHD2-2*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.49658 IGHD2-2*02 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
J SRR765688.49658 IGHJ4*02 93.18 44 3 0 0 309 352 5 48 1e-14 63.9 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23TC2GT10GT6
J SRR765688.49658 IGHJ4*01 90.91 44 4 0 0 309 352 5 48 3e-12 56.0 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 18GA4TC2GT10GT6
J SRR765688.49658 IGHJ4*03 88.64 44 5 0 0 309 352 5 48 7e-10 48.1 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 18GA2AG1TC2GT10GT6
# IGBLASTN 2.2.29+
# Query: SRR765688.31392 HQP9GKT01BBVPS length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=7
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GAT TCGACACCGGCCT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 23 1 0 95.8
FR3-IMGT 162 275 114 111 3 0 97.4
CDR3-IMGT (germline) 276 282 7 7 0 0 100
Total N/A N/A 262 249 13 0 95
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.31392 IGHV7-4-1*02 95.04 262 13 0 0 21 282 34 295 2e-104 369 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 38CT11AC2TCTA3CG1CT3TC3AG39GA18TA64AG3CG27GA37
V SRR765688.31392 IGHV7-4-1*04 94.66 262 14 0 0 21 282 34 295 1e-103 366 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 38CT11AC2TCTA3CG1CT3TC3AG39GA18TA64AG3CG2CT24GA37
V SRR765688.31392 IGHV7-4-1*01 94.64 261 14 0 0 21 281 34 294 4e-103 364 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 38CT11AC2TCTA3CG1CT3TC3AG39GA18TA64AG3CG19AT7GA36
D SRR765688.31392 IGHD2-15*01 100.00 5 0 0 0 281 285 1 5 175 10.4 AGGAT AGGAT 5
D SRR765688.31392 IGHD2-2*01 100.00 5 0 0 0 281 285 1 5 175 10.4 AGGAT AGGAT 5
D SRR765688.31392 IGHD2-2*02 100.00 5 0 0 0 281 285 1 5 175 10.4 AGGAT AGGAT 5
J SRR765688.31392 IGHJ4*02 100.00 35 0 0 0 299 333 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.31392 IGHJ5*02 100.00 35 0 0 0 299 333 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.31392 IGHJ1*01 97.14 35 1 0 0 299 333 18 52 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.11728 HQP9GKT01AL5DZ length=389|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG GAATATAAGGCGCTCCGATAATACTGC TTATTAT GCCGAGTAT TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 37 2 0 94.9
CDR1-IMGT 60 83 24 19 5 0 79.2
FR2-IMGT 84 134 51 45 6 0 88.2
CDR2-IMGT 135 158 24 22 2 0 91.7
FR3-IMGT 159 272 114 98 16 0 86
CDR3-IMGT (germline) 273 277 5 4 1 0 80
Total N/A N/A 257 225 32 0 87.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.11728 IGHV5-51*01 87.55 257 32 0 0 21 277 37 293 3e-84 302 AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG AAGCCCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 20AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.11728 IGHV5-51*03 87.16 257 33 0 0 21 277 37 293 2e-83 299 AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG AAGCCGGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 5CG14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.11728 IGHV5-51*02 86.77 257 34 0 0 21 277 37 293 2e-82 296 AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG AAGCCCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGACCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCTTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 20AG14GT10AG4GAAC2CG3TC3CATC17CT5AG7CT4AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
D SRR765688.11728 IGHD3-10*01 100.00 7 0 0 0 305 311 22 28 12 14.4 TTATTAT TTATTAT 7
D SRR765688.11728 IGHD3-10*02 100.00 7 0 0 0 305 311 21 27 12 14.4 TTATTAT TTATTAT 7
D SRR765688.11728 IGHD3-16*01 100.00 7 0 0 0 302 308 28 34 12 14.4 TGCTTAT TGCTTAT 7
J SRR765688.11728 IGHJ4*02 96.88 32 1 0 0 321 352 15 46 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.11728 IGHJ5*02 96.88 32 1 0 0 321 352 18 49 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.11728 IGHJ1*01 93.75 32 2 0 0 321 352 19 50 7e-10 48.1 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGCACCCTGGTCACCGTCTCCTC 11AC4AT15
# IGBLASTN 2.2.29+
# Query: SRR765688.21411 HQP9GKT01CV52A length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-46*01 IGHD1-1*01,IGHD1-20*01,IGHD2-21*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TTCCTTGC ACG (AC) TACGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 22 39 18 17 1 0 94.4
CDR1-IMGT 40 63 24 24 0 0 100
FR2-IMGT 64 114 51 51 0 0 100
CDR2-IMGT 115 138 24 24 0 0 100
FR3-IMGT 139 252 114 114 0 0 100
CDR3-IMGT (germline) 253 260 8 8 0 0 100
Total N/A N/A 239 238 1 0 99.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.21411 IGHV1-46*01 99.58 239 1 0 0 22 260 58 296 5e-105 371 GTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA GTTTCCTGCAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 8TC230
V SRR765688.21411 IGHV1-46*02 99.16 239 2 0 0 22 260 58 296 5e-104 367 GTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA GTTTCCTGCAAGGCATCTGGATACACCTTCAACAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 8TC22CA207
V SRR765688.21411 IGHV1-46*03 99.16 239 2 0 0 22 260 58 296 5e-104 367 GTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA GTTTCCTGCAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCTAGAGA 8TC224GT5
D SRR765688.21411 IGHD1-1*01 100.00 5 0 0 0 269 273 13 17 169 10.4 ACGAC ACGAC 5
D SRR765688.21411 IGHD1-20*01 100.00 5 0 0 0 269 273 13 17 169 10.4 ACGAC ACGAC 5
D SRR765688.21411 IGHD2-21*01 100.00 5 0 0 0 260 264 24 28 169 10.4 ATTCC ATTCC 5
J SRR765688.21411 IGHJ6*02 98.00 50 1 0 0 272 321 13 62 5e-23 91.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 42CT7
J SRR765688.21411 IGHJ6*01 96.08 51 2 0 0 272 322 13 63 3e-21 85.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAG ACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 22CG19CT8
J SRR765688.21411 IGHJ6*04 96.08 51 2 0 0 272 322 13 63 3e-21 85.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAG ACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 23CA18CT8
# IGBLASTN 2.2.29+
# Query: SRR765688.46885 HQP9GKT01B8Z8I length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=7
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TTCGA CCCTCCAACCGC GTATAGCAGCA CCTGAGTT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 23 62 40 39 1 0 97.5
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 21 3 0 87.5
FR3-IMGT 162 275 114 104 10 0 91.2
CDR3-IMGT (germline) 276 279 4 3 1 0 75
Total N/A N/A 257 237 20 0 92.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46885 IGHV7-4-1*02 92.22 257 20 0 0 23 279 36 292 1e-95 339 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC42GA8AG14AG1AG9TG3
V SRR765688.46885 IGHV7-4-1*01 91.83 257 21 0 0 23 279 36 292 1e-94 336 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC39AT2GA8AG14AG1AG9TG3
V SRR765688.46885 IGHV7-4-1*04 91.83 257 21 0 0 23 279 36 292 1e-94 336 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC22CT19GA8AG14AG1AG9TG3
D SRR765688.46885 IGHD6-13*01 100.00 11 0 0 0 292 302 3 13 0.045 22.4 GTATAGCAGCA GTATAGCAGCA 11
D SRR765688.46885 IGHD6-25*01 100.00 10 0 0 0 292 301 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
D SRR765688.46885 IGHD6-6*01 100.00 10 0 0 0 292 301 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
J SRR765688.46885 IGHJ4*02 100.00 35 0 0 0 311 345 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.46885 IGHJ5*02 100.00 35 0 0 0 311 345 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.46885 IGHJ1*01 97.14 35 1 0 0 311 345 18 52 5e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.15716 HQP9GKT01BPKZR length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD4-11*01,IGHD4-4*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA ATG GACTACA CTCATTACCAT TACTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 19 5 0 79.2
FR2-IMGT 87 137 51 48 3 0 94.1
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 103 11 0 90.4
CDR3-IMGT (germline) 276 281 6 6 0 0 100
Total N/A N/A 261 237 24 0 90.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.15716 IGHV7-4-1*02 90.80 261 24 0 0 21 281 34 294 1e-93 333 AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 26GT22TCTCCT6AGTC15CG23TC10AG9GA16GCTC1TATA4CT40GC9AC19AG1GA5GA2AG14AG1TG5TC9
V SRR765688.15716 IGHV7-4-1*01 90.42 261 25 0 0 21 281 34 294 9e-93 330 AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 26GT22TCTCCT6AGTC15CG23TC10AG9GA16GCTC1TATA4CT40GC9AC18ATAG1GA5GA2AG14AG1TG5TC9
V SRR765688.15716 IGHV7-4-1*04 90.42 261 25 0 0 21 281 34 294 9e-93 330 AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 26GT22TCTCCT6AGTC15CG23TC10AG9GA16GCTC1TATA4CT40GC9AC1CT17AG1GA5GA2AG14AG1TG5TC9
D SRR765688.15716 IGHD4-11*01 100.00 7 0 0 0 285 291 2 8 11 14.4 GACTACA GACTACA 7
D SRR765688.15716 IGHD4-4*01 100.00 7 0 0 0 285 291 2 8 11 14.4 GACTACA GACTACA 7
D SRR765688.15716 IGHD3/OR15-3a*01 100.00 6 0 0 0 283 288 14 19 45 12.4 TGGACT TGGACT 6
J SRR765688.15716 IGHJ4*02 100.00 37 0 0 0 303 339 12 48 1e-17 73.8 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 37
J SRR765688.15716 IGHJ5*02 100.00 35 0 0 0 305 339 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.15716 IGHJ4*01 97.30 37 1 0 0 303 339 12 48 3e-15 65.9 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 11GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.28724 HQP9GKT01CM7HO length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*01,IGHV1-69*12,IGHV1-69D*01 IGHD6-13*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGTCCCA AGCAGCTG AT TACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 39 0 0 100
CDR1-IMGT 60 83 24 21 3 0 87.5
FR2-IMGT 84 134 51 51 0 0 100
CDR2-IMGT 135 158 24 23 1 0 95.8
FR3-IMGT 159 272 114 99 15 0 86.8
CDR3-IMGT (germline) 273 279 7 7 0 0 100
Total N/A N/A 259 240 19 0 92.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.28724 IGHV1-69*01 92.66 259 19 0 0 21 279 37 295 2e-97 346 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 47GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.28724 IGHV1-69*12 92.66 259 19 0 0 21 279 37 295 2e-97 346 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 47GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.28724 IGHV1-69D*01 92.66 259 19 0 0 21 279 37 295 2e-97 346 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 47GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
D SRR765688.28724 IGHD6-13*01 100.00 8 0 0 0 287 294 10 17 2.9 16.4 AGCAGCTG AGCAGCTG 8
D SRR765688.28724 IGHD3-16*01 100.00 7 0 0 0 293 299 7 13 12 14.4 TGATTAC TGATTAC 7
D SRR765688.28724 IGHD3-16*02 100.00 7 0 0 0 293 299 7 13 12 14.4 TGATTAC TGATTAC 7
J SRR765688.28724 IGHJ6*02 98.04 51 1 0 0 297 347 9 59 1e-23 93.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCC 38AG12
J SRR765688.28724 IGHJ6*01 96.08 51 2 0 0 297 347 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCC 26CG11AG12
J SRR765688.28724 IGHJ6*04 96.08 51 2 0 0 297 347 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCC 27CA10AG12
# IGBLASTN 2.2.29+
# Query: SRR765688.35930 HQP9GKT01CJLTL length=406|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-18*04 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01,IGHD3-10*01 IGHJ6*02 VH Yes Out-of-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGCG (AGA) ATAT CGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATC TCTGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 39 0 0 100
CDR1-IMGT 60 83 24 18 6 0 75
FR2-IMGT 84 134 51 50 1 0 98
CDR2-IMGT 135 158 24 22 2 0 91.7
FR3-IMGT 159 272 114 110 4 0 96.5
CDR3-IMGT (germline) 273 278 6 6 0 0 100
Total N/A N/A 258 245 13 0 95
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.35930 IGHV1-18*04 94.96 258 13 0 0 21 278 37 294 1e-102 363 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGA 41AT4GC3CT4CGTC3AG6CG53TC10GA7TC12TC39CG53TA10
V SRR765688.35930 IGHV1-18*01 94.57 258 14 0 0 21 278 37 294 1e-101 360 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGA 41AT4GC3CT4CGTC2CTAG6CG53TC10GA7TC12TC39CG53TA10
V SRR765688.35930 IGHV1-18*03 94.19 258 15 0 0 21 278 37 294 1e-100 357 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACATGGCCGTGTATTACTGTGCGAGA 41AT4GC3CT4CGTC2CTAG6CG53TC10GA7TC12TC39CG41CT11TA10
D SRR765688.35930 IGHD2/OR15-2a*01 100.00 7 0 0 0 276 282 1 7 12 14.4 AGAATAT AGAATAT 7
D SRR765688.35930 IGHD2/OR15-2b*01 100.00 7 0 0 0 276 282 1 7 12 14.4 AGAATAT AGAATAT 7
D SRR765688.35930 IGHD3-10*01 100.00 7 0 0 0 291 297 24 30 12 14.4 ATTATAA ATTATAA 7
J SRR765688.35930 IGHJ6*02 100.00 35 0 0 0 336 370 28 62 2e-16 69.9 TCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA TCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 35
J SRR765688.35930 IGHJ6*01 97.22 36 1 0 0 336 371 28 63 1e-14 63.9 TCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 7CG28
J SRR765688.35930 IGHJ6*04 97.22 36 1 0 0 336 371 28 63 1e-14 63.9 TCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 8CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.30106 HQP9GKT01CBSGA length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD3-3*01,IGHD3-3*02,IGHD3/OR15-3a*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA CTTGACGGCAGCGATAGGGAC TTT (TGG) GGCCA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 38 4 0 90.5
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 19 5 0 79.2
FR3-IMGT 162 275 114 102 12 0 89.5
CDR3-IMGT (germline) 276 281 6 6 0 0 100
Total N/A N/A 261 234 27 0 89.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.30106 IGHV7-4-1*02 89.66 261 27 0 0 21 281 34 294 7e-91 324 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG8CA2AG20TACGGC3TG2GA46CA5TCCA5TA7AG1GA6CG10AG25GT6GC30TC1AGTCTC10AG7GC1AG1TG15
V SRR765688.30106 IGHV7-4-1*01 89.27 261 28 0 0 21 281 34 294 6e-90 321 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG8CA2AG20TACGGC3TG2GA46CA5TCCA5TA7AG1GA6CG10AG25GT6GC28AT1TC1AGTCTC10AG7GC1AG1TG15
V SRR765688.30106 IGHV7-4-1*04 89.27 261 28 0 0 21 281 34 294 6e-90 321 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG8CA2AG20TACGGC3TG2GA46CA5TCCA5TA7AG1GA6CG10AG25GT6GC11CT18TC1AGTCTC10AG7GC1AG1TG15
D SRR765688.30106 IGHD3-3*01 100.00 6 0 0 0 303 308 11 16 45 12.4 TTTTGG TTTTGG 6
D SRR765688.30106 IGHD3-3*02 100.00 6 0 0 0 303 308 11 16 45 12.4 TTTTGG TTTTGG 6
D SRR765688.30106 IGHD3/OR15-3a*01 100.00 6 0 0 0 303 308 11 16 45 12.4 TTTTGG TTTTGG 6
J SRR765688.30106 IGHJ4*02 97.06 34 1 0 0 306 339 15 48 2e-13 60.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 16AT17
J SRR765688.30106 IGHJ5*02 97.06 34 1 0 0 306 339 18 51 2e-13 60.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 16AT17
J SRR765688.30106 IGHJ1*01 94.12 34 2 0 0 306 339 19 52 4e-11 52.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 11AC4AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.42360 HQP9GKT01B8L6A length=364|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-5*02 IGHD1-20*01,IGHD1-7*01,IGHD1/OR15-1a*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
ACACA CT GGTATGACTGGAA TTATTCCGAT CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 22 39 18 18 0 0 100
CDR1-IMGT 40 69 30 27 3 0 90
FR2-IMGT 70 120 51 50 1 0 98
CDR2-IMGT 121 141 21 21 0 0 100
FR3-IMGT 142 249 114 101 7 6 88.6
CDR3-IMGT (germline) 250 256 7 6 1 0 85.7
Total N/A N/A 241 223 12 6 92.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.42360 IGHV2-5*02 92.53 241 12 1 6 22 256 58 298 4e-88 315 CTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACA CTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 31CG5CG3CA20CT65TC10GA2AG15TC13-A-A-A-A-C-C10CT9GA8TC26AG6
V SRR765688.42360 IGHV2-5*01 92.12 241 13 1 6 22 256 58 298 4e-87 311 CTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACA CTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGAATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 31CG5CG3CA20CT45GA19TC10GA2AG15TC13-A-A-A-A-C-C10CT9GA8TC26AG6
V SRR765688.42360 IGHV2-5*05 92.12 241 13 1 6 22 256 58 298 4e-87 311 CTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACA CTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACGGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 31CG5CG3CA20CT63AG1TC10GA2AG15TC13-A-A-A-A-C-C10CT9GA8TC26AG6
D SRR765688.42360 IGHD1-20*01 92.31 13 1 0 0 259 271 1 13 2.7 16.4 GGTATGACTGGAA GGTATAACTGGAA 5GA7
D SRR765688.42360 IGHD1-7*01 92.31 13 1 0 0 259 271 1 13 2.7 16.4 GGTATGACTGGAA GGTATAACTGGAA 5GA7
D SRR765688.42360 IGHD1/OR15-1a*01 92.31 13 1 0 0 259 271 1 13 2.7 16.4 GGTATGACTGGAA GGTATAACTGGAA 5GA7
J SRR765688.42360 IGHJ4*02 95.74 47 2 0 0 282 328 2 48 7e-19 77.8 CTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG CTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 25GA13GC7
J SRR765688.42360 IGHJ4*01 93.62 47 3 0 0 282 328 2 48 2e-16 69.9 CTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG CTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 21GA3GA13GC7
J SRR765688.42360 IGHJ4*03 91.49 47 4 0 0 282 328 2 48 4e-14 61.9 CTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG CTACTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 21GA2AGGA13GC7
# IGBLASTN 2.2.29+
# Query: SRR765688.38856 HQP9GKT01A7EJO length=362|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD2-15*01,IGHD3-3*01,IGHD3-3*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCAAG GGCTTGG AGTGGTGGTA ATTACC ACTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 29 1 0 96.7
FR2-IMGT 63 112 51 49 1 1 96.1
CDR2-IMGT 113 139 27 26 1 0 96.3
FR3-IMGT 140 253 114 101 13 0 88.6
CDR3-IMGT (germline) 254 258 5 5 0 0 100
Total N/A N/A 240 223 16 1 92.9
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.38856 IGHV6-1*01 92.92 240 16 1 1 20 258 63 302 1e-88 316 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAG 40AG5CA42-G19CA20CGCTCA6GACA13GA14GT3GA7GT11TC28ATCG1TG14
V SRR765688.38856 IGHV6-1*02 92.92 240 16 1 1 20 258 63 302 1e-88 316 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAG 40AG5CA42-G19CA20CGCTCA6GACA13GA14GT3GA7GT11TC28ATCG1TG14
V SRR765688.38856 IGHV4-4*08 66.82 220 66 4 7 40 258 77 290 5e-21 92.2 GTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATGAT-TATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAG GTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGG-GTATAT-CTATA----CCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAG 3TG3TC1GT2AGCT3GTCATCATCATC3CAAG7AC5TC5TGCG2GAAG2CA2TG6CA1GT1AGA-1GT1CT2A-3CT1G-G-T-C-7TG1TGAC1TCGC1TA-C2TCGACAAC5TCGC1GCGACAAG6AG1AC3GA1CAATAC1CGCT6AGGT3GA7GT8CA2TC3AG9TCCG3ACGA8ACCG1TG11AG2
D SRR765688.38856 IGHD2-15*01 100.00 10 0 0 0 266 275 11 20 0.17 20.4 AGTGGTGGTA AGTGGTGGTA 10
D SRR765688.38856 IGHD3-3*01 100.00 10 0 0 0 262 271 13 22 0.17 20.4 TTGGAGTGGT TTGGAGTGGT 10
D SRR765688.38856 IGHD3-3*02 100.00 10 0 0 0 262 271 13 22 0.17 20.4 TTGGAGTGGT TTGGAGTGGT 10
J SRR765688.38856 IGHJ4*02 100.00 45 0 0 0 282 326 4 48 2e-22 89.7 ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 45
J SRR765688.38856 IGHJ4*01 97.78 45 1 0 0 282 326 4 48 5e-20 81.8 ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 19GA25
J SRR765688.38856 IGHJ4*03 95.56 45 2 0 0 282 326 4 48 1e-17 73.8 ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 19GA2AG22
# IGBLASTN 2.2.29+
# Query: SRR765688.23240 HQP9GKT01BYYSD length=378|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGAG GTGGCTG AGTCGGCCGGCCAAG GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 18 6 0 75
FR2-IMGT 80 130 51 47 4 0 92.2
CDR2-IMGT 131 154 24 24 0 0 100
FR3-IMGT 155 268 114 100 14 0 87.7
CDR3-IMGT (germline) 269 275 7 7 0 0 100
Total N/A N/A 255 229 26 0 89.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.23240 IGHV7-4-1*02 89.80 255 26 0 0 21 275 41 295 5e-89 318 CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG CTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 17AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC13TA8AG11AG5TC10
V SRR765688.23240 IGHV7-4-1*04 89.41 255 27 0 0 21 275 41 295 4e-88 315 CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG CTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 17AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC1CT10GC13TA8AG11AG5TC10
V SRR765688.23240 IGHV7-4-1*01 89.37 254 27 0 0 21 274 41 294 1e-87 313 CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGA CTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 17AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC5AT7TA8AG11AG5TC9
D SRR765688.23240 IGHD6-19*01 100.00 7 0 0 0 280 286 11 17 11 14.4 GTGGCTG GTGGCTG 7
D SRR765688.23240 IGHD5-12*01 100.00 6 0 0 0 280 285 10 15 45 12.4 GTGGCT GTGGCT 6
D SRR765688.23240 IGHD1-26*01 100.00 5 0 0 0 275 279 9 13 179 10.4 GGGAG GGGAG 5
J SRR765688.23240 IGHJ4*02 97.50 40 1 0 0 302 341 9 48 5e-17 71.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22AT17
J SRR765688.23240 IGHJ4*01 95.00 40 2 0 0 302 341 9 48 1e-14 63.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14GA7AT17
J SRR765688.23240 IGHJ5*02 97.14 35 1 0 0 307 341 17 51 4e-14 61.9 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.45110 HQP9GKT01BTIR2 length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD7-27*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG TCGAACAC CTGGGGA GG GGTAT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 62 30 24 6 0 80
FR2-IMGT 63 113 51 51 0 0 100
CDR2-IMGT 114 140 27 23 4 0 85.2
FR3-IMGT 141 254 114 111 3 0 97.4
CDR3-IMGT (germline) 255 261 7 6 1 0 85.7
Total N/A N/A 242 227 15 0 93.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.45110 IGHV6-1*01 93.80 242 15 0 0 20 261 63 304 3e-93 332 CTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 7GA7AG6AG6AG3GC1AG5GC54CA1TA17CA2CA11AT48CG11AG42AG6
V SRR765688.45110 IGHV6-1*02 93.80 242 15 0 0 20 261 63 304 3e-93 332 CTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 7GA7AG6AG6AG3GC1AG5GC54CA1TA17CA2CA11AT48CG11AG42AG6
V SRR765688.45110 IGHV4-39*02 69.35 199 55 1 6 63 261 106 298 5e-28 115 TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAG TGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTG------GGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCACTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGAG 3AGAG7AC1GC3TC5TGCG2GAAG2CG2TG6CA1GT2AG2GT1CT2TACT2CT2GTTGC-C-A-A-G-T-2TACGTC2TCGT1TC2TCGACAACGCACAG2TCGC1GC2AG6AG1AC5CAATAC1CGCT6AG21CA1AG4AG9TCCG3ACGA21AG1AG4
D SRR765688.45110 IGHD7-27*01 100.00 7 0 0 0 270 276 5 11 11 14.4 CTGGGGA CTGGGGA 7
D SRR765688.45110 IGHD3-10*01 100.00 6 0 0 0 272 277 16 21 43 12.4 GGGGAG GGGGAG 6
D SRR765688.45110 IGHD3-10*02 100.00 6 0 0 0 272 277 15 20 43 12.4 GGGGAG GGGGAG 6
J SRR765688.45110 IGHJ6*02 97.78 45 1 0 0 279 323 18 62 5e-20 81.8 GGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 6AG38
J SRR765688.45110 IGHJ6*01 95.65 46 2 0 0 279 324 18 63 3e-18 75.8 GGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG GGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 6AG10CG28
J SRR765688.45110 IGHJ6*04 95.65 46 2 0 0 279 324 18 63 3e-18 75.8 GGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG GGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 6AG11CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.41920 HQP9GKT01DGU7R length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGAG GTGGCTG AGTCGGCCGGCCAAG GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 40 2 0 95.2
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 47 4 0 92.2
CDR2-IMGT 138 161 24 24 0 0 100
FR3-IMGT 162 275 114 100 14 0 87.7
CDR3-IMGT (germline) 276 282 7 7 0 0 100
Total N/A N/A 262 236 26 0 90.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.41920 IGHV7-4-1*02 90.08 262 26 0 0 21 282 34 295 3e-92 329 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14TC12GC13TA8AG11AG5TC10
V SRR765688.41920 IGHV7-4-1*04 89.69 262 27 0 0 21 282 34 295 2e-91 325 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14TC1CT10GC13TA8AG11AG5TC10
V SRR765688.41920 IGHV7-4-1*01 89.66 261 27 0 0 21 281 34 294 7e-91 324 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14TC12GC5AT7TA8AG11AG5TC9
D SRR765688.41920 IGHD6-19*01 100.00 7 0 0 0 287 293 11 17 11 14.4 GTGGCTG GTGGCTG 7
D SRR765688.41920 IGHD5-12*01 100.00 6 0 0 0 287 292 10 15 46 12.4 GTGGCT GTGGCT 6
D SRR765688.41920 IGHD1-26*01 100.00 5 0 0 0 282 286 9 13 183 10.4 GGGAG GGGAG 5
J SRR765688.41920 IGHJ4*02 97.50 40 1 0 0 309 348 9 48 5e-17 71.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22AT17
J SRR765688.41920 IGHJ4*01 95.00 40 2 0 0 309 348 9 48 1e-14 63.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14GA7AT17
J SRR765688.41920 IGHJ5*02 97.14 35 1 0 0 314 348 17 51 5e-14 61.9 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.5099 HQP9GKT01AS88P length=374|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-25*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA CTCGACG GCAGCG ATAGG GACTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 39 3 0 92.9
CDR1-IMGT 63 86 24 21 3 0 87.5
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 105 9 0 92.1
CDR3-IMGT (germline) 276 281 6 6 0 0 100
Total N/A N/A 261 240 21 0 92
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.5099 IGHV7-4-1*02 91.95 261 21 0 0 21 281 34 294 2e-96 343 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG20TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT46TC6AG4AG5CT3AG1TG15
V SRR765688.5099 IGHV7-4-1*01 91.57 261 22 0 0 21 281 34 294 1e-95 339 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG20TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT41AT4TC6AG4AG5CT3AG1TG15
V SRR765688.5099 IGHV7-4-1*04 91.57 261 22 0 0 21 281 34 294 1e-95 339 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG20TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT24CT21TC6AG4AG5CT3AG1TG15
D SRR765688.5099 IGHD6-25*01 100.00 6 0 0 0 289 294 8 13 45 12.4 GCAGCG GCAGCG 6
D SRR765688.5099 IGHD3-22*01 100.00 5 0 0 0 294 298 11 15 178 10.4 GATAG GATAG 5
D SRR765688.5099 IGHD3-9*01 100.00 5 0 0 0 293 297 7 11 178 10.4 CGATA CGATA 5
J SRR765688.5099 IGHJ4*02 95.00 40 2 0 0 300 339 9 48 1e-14 63.9 GACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4TA17AT17
J SRR765688.5099 IGHJ5*02 97.14 35 1 0 0 305 339 17 51 4e-14 61.9 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17AT17
J SRR765688.5099 IGHJ4*01 92.50 40 3 0 0 300 339 9 48 3e-12 56.0 GACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 4TA9GA7AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.41370 HQP9GKT01DK3O2 length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD3-10*01,IGHD3-10*02,IGHD3-3*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCTAA ATTATA TACAC TTCGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 37 5 0 88.1
CDR1-IMGT 63 86 24 15 9 0 62.5
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 16 8 0 66.7
FR3-IMGT 162 275 114 101 13 0 88.6
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 226 37 0 85.9
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.41370 IGHV7-4-1*02 85.93 263 37 0 0 21 283 34 296 2e-82 296 AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 3GA18CA1AG14ATTC2AG5AC5ACATTA1TC1GA1AGGC3AG6CG46TCGATC3TC3AG1AG1GAGC5AG7CA7GA7AG18GA14AG1TA16AG3CA3AG4AG11TG5TC11
V SRR765688.41370 IGHV7-4-1*04 85.55 263 38 0 0 21 283 34 296 2e-81 293 AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 3GA18CA1AG14ATTC2AG5AC5ACATTA1TC1GA1AGGC3AG6CG46TCGATC3TC3AG1AG1GAGC5AG7CA7GA7AG18GA12CT1AG1TA16AG3CA3AG4AG11TG5TC11
V SRR765688.41370 IGHV7-4-1*01 85.44 261 38 0 0 21 281 34 294 1e-80 290 AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 3GA18CA1AG14ATTC2AG5AC5ACATTA1TC1GA1AGGC3AG6CG46TCGATC3TC3AG1AG1GAGC5AG7CA7GA7AG18GA14AG1TA12AT3AG3CA3AG4AG11TG5TC9
D SRR765688.41370 IGHD3-10*01 100.00 6 0 0 0 289 294 24 29 45 12.4 ATTATA ATTATA 6
D SRR765688.41370 IGHD3-10*02 100.00 6 0 0 0 289 294 23 28 45 12.4 ATTATA ATTATA 6
D SRR765688.41370 IGHD3-3*01 100.00 6 0 0 0 289 294 24 29 45 12.4 ATTATA ATTATA 6
J SRR765688.41370 IGHJ5*02 100.00 43 0 0 0 300 342 9 51 3e-21 85.7 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 43
J SRR765688.41370 IGHJ4*02 100.00 35 0 0 0 308 342 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.41370 IGHJ5*01 95.35 43 2 0 0 300 342 9 51 2e-16 69.9 TTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 6CT10GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.46467 HQP9GKT01EZF79 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-46*01 IGHD1-1*01,IGHD1-20*01,IGHD2-21*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TTCCTTGC ACG (AC) TACGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 38 1 0 97.4
CDR1-IMGT 60 83 24 24 0 0 100
FR2-IMGT 84 134 51 51 0 0 100
CDR2-IMGT 135 158 24 24 0 0 100
FR3-IMGT 159 272 114 114 0 0 100
CDR3-IMGT (germline) 273 280 8 8 0 0 100
Total N/A N/A 260 259 1 0 99.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46467 IGHV1-46*01 99.62 260 1 0 0 21 280 37 296 8e-115 403 AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 29TC230
V SRR765688.46467 IGHV1-46*02 99.23 260 2 0 0 21 280 37 296 7e-114 400 AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCATCTGGATACACCTTCAACAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 29TC22CA207
V SRR765688.46467 IGHV1-46*03 99.23 260 2 0 0 21 280 37 296 7e-114 400 AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCTAGAGA 29TC224GT5
D SRR765688.46467 IGHD1-1*01 100.00 5 0 0 0 289 293 13 17 180 10.4 ACGAC ACGAC 5
D SRR765688.46467 IGHD1-20*01 100.00 5 0 0 0 289 293 13 17 180 10.4 ACGAC ACGAC 5
D SRR765688.46467 IGHD2-21*01 100.00 5 0 0 0 280 284 24 28 180 10.4 ATTCC ATTCC 5
J SRR765688.46467 IGHJ6*02 98.00 50 1 0 0 292 341 13 62 5e-23 91.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 42CT7
J SRR765688.46467 IGHJ6*01 96.08 51 2 0 0 292 342 13 63 3e-21 85.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAG ACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 22CG19CT8
J SRR765688.46467 IGHJ6*04 96.08 51 2 0 0 292 342 13 63 3e-21 85.7 ACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAG ACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 23CA18CT8
# IGBLASTN 2.2.29+
# Query: SRR765688.17960 HQP9GKT01CN8ZX length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-70D*04 IGHD2-21*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGCAT CGCGGGAGGTGAGGG GACTGCT N/A TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 39 19 19 0 0 100
CDR1-IMGT 40 69 30 23 7 0 76.7
FR2-IMGT 70 120 51 47 4 0 92.2
CDR2-IMGT 121 141 21 20 1 0 95.2
FR3-IMGT 142 255 114 108 6 0 94.7
CDR3-IMGT (germline) 256 263 8 7 1 0 87.5
Total N/A N/A 243 224 19 0 92.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.17960 IGHV2-70D*04 92.18 243 19 0 0 21 263 57 299 6e-90 321 ACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCAT ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGCGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATTCTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGAT 21CG2TC8TC1GC1CA2AGCG10GA7TC9TC13AG30TG12AG11GA10TA23GA25TC10CT27CG2
V SRR765688.17960 IGHV2-70D*14 91.77 243 20 0 0 21 263 57 299 5e-89 318 ACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCAT ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGCGTGTGAGCTGGATCCGTCAGCCCCCAGGTAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATTCTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGAT 21CG2TC8TC1GC1CA2AGCG10GA7TC9TC4GT8AG30TG12AG11GA10TA23GA25TC10CT27CG2
V SRR765688.17960 IGHV2-70*10 91.36 243 21 0 0 21 263 57 299 4e-88 315 ACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCAT ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGCGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGATTGCACGCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGAT 21CG2TC8TC1GC1CA2AGCG10GA7TC9TC13AG6CA23TG6TA5AG11GA10TA23GA25TC10CT27CG2
D SRR765688.17960 IGHD2-21*02 100.00 7 0 0 0 279 285 17 23 11 14.4 GACTGCT GACTGCT 7
D SRR765688.17960 IGHD3/OR15-3a*01 100.00 6 0 0 0 278 283 15 20 43 12.4 GGACTG GGACTG 6
D SRR765688.17960 IGHD3/OR15-3b*01 100.00 6 0 0 0 278 283 15 20 43 12.4 GGACTG GGACTG 6
J SRR765688.17960 IGHJ4*02 95.35 43 2 0 0 286 328 6 48 2e-16 69.9 TTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22TC9TC10
J SRR765688.17960 IGHJ4*01 93.02 43 3 0 0 286 328 6 48 4e-14 61.9 TTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 17GA4TC9TC10
J SRR765688.17960 IGHJ5*02 94.29 35 2 0 0 294 328 17 51 1e-11 54.0 CTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 14TC9TC10
# IGBLASTN 2.2.29+
# Query: SRR765688.25497 HQP9GKT01B88YS length=389|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-39*01 IGHD1-7*01 IGHJ4*02 VH Yes Out-of-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA ATGGGATGATACTG GGAACTAC TACGCCCGGGCT CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 76 30 28 2 0 93.3
FR2-IMGT 77 126 51 49 1 1 96.1
CDR2-IMGT 127 144 21 17 1 3 81
FR3-IMGT 145 258 114 108 6 0 94.7
CDR3-IMGT (germline) 259 266 8 8 0 0 100
Total N/A N/A 250 235 11 4 94
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.25497 IGHV4-39*01 94.00 250 11 2 4 21 266 50 299 3e-93 332 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGACA 17GA12AG20GA16-C33CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17
V SRR765688.25497 IGHV4-39*02 93.55 248 12 2 4 21 264 50 297 2e-91 325 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCACTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCGAGA 17GA12AG20GA16-C33CG10-T-A-T8TC8TC29GA11CG11GC11CG5GC26AG15
V SRR765688.25497 IGHV4-39*05 93.47 245 12 2 4 21 261 50 294 6e-90 321 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCG CCCCGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCTGTGTATTACTGTGCG 3TC13GA12AG20GA16-C33CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG12
D SRR765688.25497 IGHD1-7*01 100.00 8 0 0 0 281 288 10 17 2.9 16.4 GGAACTAC GGAACTAC 8
D SRR765688.25497 IGHD1-26*01 92.31 13 1 0 0 279 291 8 20 2.9 16.4 TGGGAACTACTAC TGGGAGCTACTAC 5AG7
D SRR765688.25497 IGHD2-8*02 100.00 6 0 0 0 276 281 10 15 46 12.4 TACTGG TACTGG 6
J SRR765688.25497 IGHJ4*02 95.74 47 2 0 0 301 347 2 48 8e-19 77.8 CTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 11CATC34
J SRR765688.25497 IGHJ4*01 93.62 47 3 0 0 301 347 2 48 2e-16 69.9 CTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 11CATC8GA25
J SRR765688.25497 IGHJ5*02 100.00 34 0 0 0 314 347 18 51 7e-16 67.9 TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 34
# IGBLASTN 2.2.29+
# Query: SRR765688.39627 HQP9GKT01CATH4 length=374|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*08 IGHD2-15*01,IGHD3-22*01,IGHD3-3*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGACA TGGAA AGTGGT CGGACCAGGGA TGGTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 22 47 26 24 2 0 92.3
CDR1-IMGT 48 71 24 21 3 0 87.5
FR2-IMGT 72 122 51 42 9 0 82.4
CDR2-IMGT 123 143 21 15 6 0 71.4
FR3-IMGT 144 257 114 100 14 0 87.7
CDR3-IMGT (germline) 258 265 8 5 3 0 62.5
Total N/A N/A 244 207 37 0 84.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.39627 IGHV4-59*08 84.84 244 37 0 0 22 265 50 293 1e-73 266 CCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTGTTCGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACA 7GC4TC26TG1GAAG9CG2TC8TG3AC4TA1CG1CA4CA14TG11TCGAAG3AG1CG3GC1CA17GA6CA1CT12TA15GC2AT8GA1CG4CG25AGCTAG5TC4TCTGCA4
V SRR765688.39627 IGHV4-59*01 84.81 237 36 0 0 22 258 50 286 3e-71 258 CCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTG 7GC4TC26TG1GAAG9CG2TC8TG3AC4TA1CG1CA4CA14TG11TCGAAG3AG1CG3GC1CA17GA6CA1CT12TA15GC2AT8GA1CG4CG12CT2AG9AGCTAG5TC4
V SRR765688.39627 IGHV4-59*07 84.81 237 36 0 0 22 258 50 286 3e-71 258 CCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTG 7GC4TC26TG1GAAG9CG2TC8TG3AC4TA1CG1CA4CA14TG11TCGAAG3AG1CG3GC1CA17GA6CA1CT12TA15GC2AT8GA1CG4CG12CT2AG9AGCTAG5TC4
D SRR765688.39627 IGHD2-15*01 100.00 6 0 0 0 271 276 11 16 44 12.4 AGTGGT AGTGGT 6
D SRR765688.39627 IGHD3-22*01 100.00 6 0 0 0 271 276 17 22 44 12.4 AGTGGT AGTGGT 6
D SRR765688.39627 IGHD3-3*01 100.00 6 0 0 0 271 276 17 22 44 12.4 AGTGGT AGTGGT 6
J SRR765688.39627 IGHJ5*02 97.83 46 1 0 0 288 333 6 51 1e-20 83.8 TGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG TGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 36AG9
J SRR765688.39627 IGHJ5*01 93.48 46 3 0 0 288 333 6 51 7e-16 67.9 TGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG TGGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 9CT10GA15AG9
J SRR765688.39627 IGHJ4*02 97.14 35 1 0 0 299 333 14 48 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 25AG9
# IGBLASTN 2.2.29+
# Query: SRR765688.48095 HQP9GKT01DC6RH length=389|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR4|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-31*03 IGHD3-10*01,IGHD3-10*02 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG CGAGAGATGACTATAC TTCGGGGAGTTATTA CG GGTTC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 54 35 35 0 0 100
CDR1-IMGT 55 84 30 27 3 0 90
FR2-IMGT 85 135 51 48 3 0 94.1
CDR2-IMGT 136 156 21 18 3 0 85.7
FR3-IMGT 157 270 114 106 8 0 93
CDR3-IMGT (germline) 271 277 7 7 0 0 100
Total N/A N/A 258 241 17 0 93.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.48095 IGHV4-31*03 93.41 258 17 0 0 20 277 41 298 8e-99 350 CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAG CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGAG 54AG5CATC7AG33AG8AG7CA5CG5AG6TC5TC26TA23AG2TC8AGTC31CG16
V SRR765688.48095 IGHV4-31*01 93.02 258 18 0 0 20 277 41 298 7e-98 347 CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAG CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCTAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGAG 54AG5CATC7AG33AG8AG7CA5CG5AG6TC5TC16GT9TA23AG2TC8AGTC31CG16
V SRR765688.48095 IGHV4-31*02 93.02 258 18 0 0 20 277 41 298 7e-98 347 CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAG CTTCACAGACCCTGTCCCTCACCTGTACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGAG 25CT28AG5CATC7AG33AG8AG7CA5CG5AG6TC5TC26TA23AG2TC8AGTC31CG16
D SRR765688.48095 IGHD3-10*01 100.00 15 0 0 0 294 308 13 27 2e-04 30.4 TTCGGGGAGTTATTA TTCGGGGAGTTATTA 15
D SRR765688.48095 IGHD3-10*02 100.00 15 0 0 0 294 308 12 26 2e-04 30.4 TTCGGGGAGTTATTA TTCGGGGAGTTATTA 15
D SRR765688.48095 IGHD3-16*01 100.00 10 0 0 0 297 306 19 28 0.18 20.4 GGGGAGTTAT GGGGAGTTAT 10
J SRR765688.48095 IGHJ5*02 97.78 45 1 0 0 311 355 7 51 5e-20 81.8 GGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG GGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23TA21
J SRR765688.48095 IGHJ5*01 93.33 45 3 0 0 311 355 7 51 3e-15 65.9 GGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG GGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 8CT10GA3TA21
J SRR765688.48095 IGHJ4*02 97.14 35 1 0 0 321 355 14 48 5e-14 61.9 CTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 13TA21
# IGBLASTN 2.2.29+
# Query: SRR765688.32938 HQP9GKT01CBIO9 length=387|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD5-12*01,IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCGGGGGCA TGGATATAGT TATGGTTATTCTGG CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 42 0 0 100
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 24 0 0 100
FR3-IMGT 162 275 114 111 3 0 97.4
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 255 8 0 97
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.32938 IGHV7-4-1*02 96.96 263 8 0 0 21 283 34 296 1e-109 386 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 49GC5TC2AGTC6CA136CA13AG15CG29
V SRR765688.32938 IGHV7-4-1*04 96.58 263 9 0 0 21 283 34 296 1e-108 383 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 49GC5TC2AGTC6CA132CT3CA13AG15CG29
V SRR765688.32938 IGHV7-4-1*01 96.55 261 9 0 0 21 281 34 294 9e-108 380 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 49GC5TC2AGTC6CA136CA12ATAG15CG27
D SRR765688.32938 IGHD5-12*01 100.00 10 0 0 0 293 302 2 11 0.18 20.4 TGGATATAGT TGGATATAGT 10
D SRR765688.32938 IGHD5/OR15-5a*01 100.00 10 0 0 0 293 302 2 11 0.18 20.4 TGGATATAGT TGGATATAGT 10
D SRR765688.32938 IGHD5/OR15-5b*01 100.00 10 0 0 0 293 302 2 11 0.18 20.4 TGGATATAGT TGGATATAGT 10
J SRR765688.32938 IGHJ4*02 100.00 38 0 0 0 317 354 11 48 3e-18 75.8 CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 38
J SRR765688.32938 IGHJ5*02 100.00 35 0 0 0 320 354 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.32938 IGHJ4*01 97.37 38 1 0 0 317 354 11 48 8e-16 67.9 CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 12GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.33355 HQP9GKT01B9Y0F length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02,IGHJ5*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GAT TCGACACCGGCCT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 138 52 49 2 1 94.2
CDR2-IMGT 139 162 24 23 1 0 95.8
FR3-IMGT 163 276 114 111 3 0 97.4
CDR3-IMGT (germline) 277 283 7 7 0 0 100
Total N/A N/A 263 249 13 1 94.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.33355 IGHV7-4-1*02 94.68 263 13 1 1 21 283 34 295 4e-102 361 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCC-TGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 38CT11AC2TCTA3CG1CT3TC3AG20C-19GA18TA64AG3CG27GA37
V SRR765688.33355 IGHV7-4-1*04 94.30 263 14 1 1 21 283 34 295 3e-101 358 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCC-TGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 38CT11AC2TCTA3CG1CT3TC3AG20C-19GA18TA64AG3CG2CT24GA37
V SRR765688.33355 IGHV7-4-1*01 94.27 262 14 1 1 21 282 34 294 9e-101 357 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCC-TGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 38CT11AC2TCTA3CG1CT3TC3AG20C-19GA18TA64AG3CG19AT7GA36
D SRR765688.33355 IGHD2-15*01 100.00 5 0 0 0 282 286 1 5 175 10.4 AGGAT AGGAT 5
D SRR765688.33355 IGHD2-2*01 100.00 5 0 0 0 282 286 1 5 175 10.4 AGGAT AGGAT 5
D SRR765688.33355 IGHD2-2*02 100.00 5 0 0 0 282 286 1 5 175 10.4 AGGAT AGGAT 5
J SRR765688.33355 IGHJ4*02 100.00 35 0 0 0 300 334 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.33355 IGHJ5*02 100.00 35 0 0 0 300 334 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.33355 IGHJ1*01 97.14 35 1 0 0 300 334 18 52 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.32244 HQP9GKT01C73BZ length=376|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*06,IGHV1-69*14 IGHD1-7*01 IGHJ2*01 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA TCTTG GGAACTA TGCGG GGTAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 34 1 0 97.1
CDR1-IMGT 56 79 24 18 6 0 75
FR2-IMGT 80 130 51 48 3 0 94.1
CDR2-IMGT 131 154 24 18 6 0 75
FR3-IMGT 155 268 114 99 15 0 86.8
CDR3-IMGT (germline) 269 276 8 7 1 0 87.5
Total N/A N/A 256 224 32 0 87.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.32244 IGHV1-69*06 87.50 256 32 0 0 21 276 41 296 7e-84 300 CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGA CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 27GA11AG2TCTC5AC1CG5GC5CGTC26CG27GACT6AT3TGTCCACA13AG2TC9CG4CG14CA4TC1GACGTC4TC16GA6CG18AG5TC6CA4
V SRR765688.32244 IGHV1-69*14 87.50 256 32 0 0 21 276 41 296 7e-84 300 CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGA CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 27GA11AG2TCTC5AC1CG5GC5CGTC26CG27GACT6AT3TGTCCACA13AG2TC9CG4CG14CA4TC1GACGTC4TC16GA6CG18AG5TC6CA4
V SRR765688.32244 IGHV1-69*01 87.11 256 33 0 0 21 276 41 296 6e-83 297 CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGA CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGA 27GA11AG2TCTC5AC1CG5GC5CGTC26CG27GACT6AT3TGTCCACA13AG2TC9CG4CG12AG1CA4TC1GACGTC4TC16GA6CG18AG5TC6CA4
D SRR765688.32244 IGHD1-7*01 100.00 7 0 0 0 282 288 10 16 11 14.4 GGAACTA GGAACTA 7
D SRR765688.32244 IGHD2-2*01 100.00 6 0 0 0 286 291 25 30 45 12.4 CTATGC CTATGC 6
D SRR765688.32244 IGHD2-2*03 100.00 6 0 0 0 286 291 25 30 45 12.4 CTATGC CTATGC 6
J SRR765688.32244 IGHJ2*01 97.92 48 1 0 0 294 341 6 53 8e-22 87.7 GGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG GGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG 6CT41
J SRR765688.32244 IGHJ1*01 91.43 35 3 0 0 307 341 18 52 3e-09 46.1 CTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 8GATG14TC10
J SRR765688.32244 IGHJ4*01 88.57 35 4 0 0 307 341 14 48 6e-07 38.2 CTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG CTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 8GATA2CA11TC10
# IGBLASTN 2.2.29+
# Query: SRR765688.17491 HQP9GKT01BUYPI length=378|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-10-1*01,IGHV5-10-1*03 IGHD6-13*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA CACG ATAGCAGCAGCTGGTAC GTACAGG TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 35 0 0 100
CDR1-IMGT 56 79 24 24 0 0 100
FR2-IMGT 80 130 51 51 0 0 100
CDR2-IMGT 131 154 24 24 0 0 100
FR3-IMGT 155 268 114 114 0 0 100
CDR3-IMGT (germline) 269 274 6 6 0 0 100
Total N/A N/A 254 254 0 0 100
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.17491 IGHV5-10-1*01 100.00 254 0 0 0 21 274 41 294 6e-113 397 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA 254
V SRR765688.17491 IGHV5-10-1*03 100.00 254 0 0 0 21 274 41 294 6e-113 397 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA 254
V SRR765688.17491 IGHV5-10-1*04 99.61 254 1 0 0 21 274 41 294 5e-112 394 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGA 160CG93
D SRR765688.17491 IGHD6-13*01 100.00 17 0 0 0 279 295 5 21 1e-05 34.4 ATAGCAGCAGCTGGTAC ATAGCAGCAGCTGGTAC 17
D SRR765688.17491 IGHD6-19*01 100.00 8 0 0 0 288 295 14 21 2.8 16.4 GCTGGTAC GCTGGTAC 8
D SRR765688.17491 IGHD6-25*01 100.00 8 0 0 0 279 286 5 12 2.8 16.4 ATAGCAGC ATAGCAGC 8
J SRR765688.17491 IGHJ4*02 100.00 42 0 0 0 303 344 7 48 1e-20 83.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 42
J SRR765688.17491 IGHJ4*01 97.62 42 1 0 0 303 344 7 48 3e-18 75.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 16GA25
J SRR765688.17491 IGHJ5*02 100.00 35 0 0 0 310 344 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.13678 HQP9GKT01BUUUG length=369|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA GTTCCGGGCCTAAC AGTGTCT TGTTCTGGTCCGA CCCTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 20 4 0 83.3
FR2-IMGT 71 121 51 48 3 0 94.1
CDR2-IMGT 122 142 21 17 4 0 81
FR3-IMGT 143 254 114 101 11 2 88.6
CDR3-IMGT (germline) 255 262 8 8 0 0 100
Total N/A N/A 244 220 22 2 90.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.13678 IGHV4-59*08 90.16 244 22 2 2 21 262 50 293 5e-82 294 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14NC5-C7GA5AGTC3CG25CT1TA12
V SRR765688.13678 IGHV4-4*08 89.26 242 24 2 2 21 260 50 291 4e-79 285 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GAAC2TG2AG2CG4TA1TC5-C29AC1CTGA14NC5-C7GA5AGTC3CG25CT1TA10
V SRR765688.13678 IGHV4-59*01 89.26 242 24 2 2 21 260 50 291 4e-79 285 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14NC5-C7GA5AGTC3CG7CT2AG14CT1TA10
D SRR765688.13678 IGHD5/OR15-5a*01 100.00 7 0 0 0 277 283 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.13678 IGHD5/OR15-5b*01 100.00 7 0 0 0 277 283 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.13678 IGHD2-8*02 100.00 5 0 0 0 288 292 12 16 175 10.4 CTGGT CTGGT 5
J SRR765688.13678 IGHJ5*02 100.00 37 0 0 0 297 333 15 51 1e-17 73.8 CCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 37
J SRR765688.13678 IGHJ4*02 100.00 35 0 0 0 299 333 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.13678 IGHJ5*01 97.22 36 1 0 0 298 333 16 51 1e-14 63.9 CCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 10GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.7787 HQP9GKT01COO0Q length=360|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-5*02 IGHD3-16*01,IGHD3-16*02,IGHD5-24*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CACAG TGCGGGATGGCTGCC TGAT (TAC) TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 39 19 19 0 0 100
CDR1-IMGT 40 69 30 27 3 0 90
FR2-IMGT 70 120 51 48 3 0 94.1
CDR2-IMGT 121 147 27 19 2 6 70.4
FR3-IMGT 148 261 114 107 7 0 93.9
CDR3-IMGT (germline) 262 269 8 8 0 0 100
Total N/A N/A 249 228 15 6 91.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.7787 IGHV2-5*02 91.57 249 15 1 6 21 269 57 299 5e-89 318 GCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATG------ATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 21AG11TC7CG9AGCGAG62C-C-A-A-A-T-2TA1CGAC2ATCA50GA7AG4CT22CT30
V SRR765688.7787 IGHV2-5*01 91.16 249 16 1 6 21 269 57 299 4e-88 315 GCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGAATGATG------ATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 21AG11TC7CG9AGCGAG55GA6C-C-A-A-A-T-2TA1CGAC2ATCA50GA7AG4CT22CT30
V SRR765688.7787 IGHV2-5*05 91.16 249 16 1 6 21 269 57 299 4e-88 315 GCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATG------ATAAGCGCTACGGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 21AG11TC7CG9AGCGAG62C-C-A-A-A-T-2TA1CGAC2ATCA1AG48GA7AG4CT22CT30
D SRR765688.7787 IGHD3-16*01 100.00 7 0 0 0 285 291 7 13 11 14.4 TGATTAC TGATTAC 7
D SRR765688.7787 IGHD3-16*02 100.00 7 0 0 0 285 291 7 13 11 14.4 TGATTAC TGATTAC 7
D SRR765688.7787 IGHD5-24*01 100.00 7 0 0 0 275 281 6 12 11 14.4 GATGGCT GATGGCT 7
J SRR765688.7787 IGHJ4*02 97.30 37 1 0 0 289 325 12 48 3e-15 65.9 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 32TC4
J SRR765688.7787 IGHJ5*02 97.14 35 1 0 0 291 325 17 51 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 30TC4
J SRR765688.7787 IGHJ4*01 94.59 37 2 0 0 289 325 12 48 7e-13 58.0 TACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG TACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 11GA20TC4
# IGBLASTN 2.2.29+
# Query: SRR765688.43055 HQP9GKT01ANQEB length=386|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG GAATATAAGGCGCTCCGATAATACTGC TTATTAT GCCGAGTAT TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 56 36 33 2 1 91.7
CDR1-IMGT 57 80 24 19 5 0 79.2
FR2-IMGT 81 131 51 45 6 0 88.2
CDR2-IMGT 132 155 24 22 2 0 91.7
FR3-IMGT 156 269 114 98 16 0 86
CDR3-IMGT (germline) 270 274 5 4 1 0 80
Total N/A N/A 254 221 32 1 87
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.43055 IGHV5-51*01 87.01 254 32 1 1 21 274 41 293 4e-80 288 CCGGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG CCGGGGAGTCTCTGAAGA-TCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 16AG1A-13GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.43055 IGHV5-51*03 86.90 252 32 1 1 23 274 43 293 4e-79 285 GGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGA-TCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG1A-13GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.43055 IGHV5-51*04 86.51 252 33 1 1 23 274 43 293 3e-78 282 GGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGA-TCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGCCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG1A-13GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA1TC2GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
D SRR765688.43055 IGHD3-10*01 100.00 7 0 0 0 302 308 22 28 12 14.4 TTATTAT TTATTAT 7
D SRR765688.43055 IGHD3-10*02 100.00 7 0 0 0 302 308 21 27 12 14.4 TTATTAT TTATTAT 7
D SRR765688.43055 IGHD3-16*01 100.00 7 0 0 0 299 305 28 34 12 14.4 TGCTTAT TGCTTAT 7
J SRR765688.43055 IGHJ4*02 96.88 32 1 0 0 318 349 15 46 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.43055 IGHJ5*02 96.88 32 1 0 0 318 349 18 49 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.43055 IGHJ1*01 93.75 32 2 0 0 318 349 19 50 7e-10 48.1 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGCACCCTGGTCACCGTCTCCTC 11AC4AT15
# IGBLASTN 2.2.29+
# Query: SRR765688.24232 HQP9GKT01BTWAC length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG GAATATAAGGCGCTCCGATAATACCGC TTATTAT GCCGAG TACTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 19 5 0 79.2
FR2-IMGT 80 130 51 45 6 0 88.2
CDR2-IMGT 131 154 24 22 2 0 91.7
FR3-IMGT 155 268 114 97 17 0 85.1
CDR3-IMGT (germline) 269 273 5 4 1 0 80
Total N/A N/A 253 220 33 0 87
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.24232 IGHV5-51*01 86.96 253 33 0 0 21 273 41 293 2e-81 293 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAG CCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT17TC1CGTCTC10CA5CG8AC10GA9TC4GC7GC7TC7GA1AGTC8GC6AG10TC3
V SRR765688.24232 IGHV5-51*03 86.85 251 33 0 0 23 273 43 293 1e-80 290 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT17TC1CGTCTC10CA5CG8AC10GA9TC4GC7GC7TC7GA1AGTC8GC6AG10TC3
V SRR765688.24232 IGHV5-51*04 86.45 251 34 0 0 23 273 43 293 1e-79 286 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGCCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT17TC1CGTCTC10CA5CG8AC10GA1TC7TC4GC7GC7TC7GA1AGTC8GC6AG10TC3
D SRR765688.24232 IGHD3-10*01 100.00 7 0 0 0 301 307 22 28 12 14.4 TTATTAT TTATTAT 7
D SRR765688.24232 IGHD3-10*02 100.00 7 0 0 0 301 307 21 27 12 14.4 TTATTAT TTATTAT 7
D SRR765688.24232 IGHD3-16*01 100.00 7 0 0 0 302 308 2 8 12 14.4 TATTATG TATTATG 7
J SRR765688.24232 IGHJ4*02 97.14 35 1 0 0 314 348 12 46 5e-14 61.9 TACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 19AT15
J SRR765688.24232 IGHJ5*02 96.97 33 1 0 0 316 348 17 49 7e-13 58.0 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 17AT15
J SRR765688.24232 IGHJ4*01 94.29 35 2 0 0 314 348 12 46 1e-11 54.0 TACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTC 11GA7AT15
# IGBLASTN 2.2.29+
# Query: SRR765688.35786 HQP9GKT01DW0J7 length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-18*04 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCGAG GTAGTACCAG GTGGCTCGAACCCA TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 34 1 0 97.1
CDR1-IMGT 56 79 24 23 1 0 95.8
FR2-IMGT 80 130 51 50 1 0 98
CDR2-IMGT 131 154 24 23 1 0 95.8
FR3-IMGT 155 268 114 107 7 0 93.9
CDR3-IMGT (germline) 269 276 8 8 0 0 100
Total N/A N/A 256 245 11 0 95.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.35786 IGHV1-18*04 95.70 256 11 0 0 21 276 41 296 1e-103 366 CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA CTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGAGA 27CA24TC52CG22GA7GC11AGTC32CT36TG18AG5TC11
V SRR765688.35786 IGHV1-18*01 95.31 256 12 0 0 21 276 41 296 1e-102 363 CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA CTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGAGA 27CA24TC2CT49CG22GA7GC11AGTC32CT36TG18AG5TC11
V SRR765688.35786 IGHV1-18*03 94.92 256 13 0 0 21 276 41 296 1e-101 360 CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA CTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACATGGCCGTGTATTACTGTGCGAGAGA 27CA24TC2CT49CG22GA7GC11AGTC32CT36TG11CT6AG5TC11
D SRR765688.35786 IGHD2-2*01 100.00 10 0 0 0 282 291 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
D SRR765688.35786 IGHD2-2*02 100.00 10 0 0 0 282 291 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
D SRR765688.35786 IGHD2-2*03 100.00 10 0 0 0 282 291 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
J SRR765688.35786 IGHJ4*02 100.00 42 0 0 0 306 347 7 48 1e-20 83.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 42
J SRR765688.35786 IGHJ4*01 97.62 42 1 0 0 306 347 7 48 3e-18 75.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 16GA25
J SRR765688.35786 IGHJ5*02 100.00 35 0 0 0 313 347 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.45263 HQP9GKT01CZ91S length=353|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-31*03 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01 IGHJ3*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTACG (AGA) ATATTG GGGAGAGA TGATG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 62 30 28 2 0 93.3
FR2-IMGT 63 113 51 45 6 0 88.2
CDR2-IMGT 114 134 21 17 4 0 81
FR3-IMGT 135 248 114 100 14 0 87.7
CDR3-IMGT (germline) 249 254 6 5 1 0 83.3
Total N/A N/A 235 207 28 0 88.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.45263 IGHV4-31*03 88.09 235 28 0 0 20 254 63 297 9e-78 280 CTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGA CTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGA 4TA21CGTC18TACG4GA16AG20TG2TCGA3TA9TG1CG4AT11GT13CA2CTTA4TA5TA28CACG4CG4CG1AG18AG1TG9AG5
V SRR765688.45263 IGHV4-31*01 87.66 235 29 0 0 20 254 63 297 7e-77 277 CTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGA CTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCTAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGA 4TA21CGTC18TACG4GA16AG20TG2TCGA3TA9TG1CG4AT11GT12GTCA2CTTA4TA5TA28CACG4CG4CG1AG18AG1TG9AG5
V SRR765688.45263 IGHV4-31*02 87.66 235 29 0 0 20 254 63 297 7e-77 277 CTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGA CTGTACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGA 3CTTA21CGTC18TACG4GA16AG20TG2TCGA3TA9TG1CG4AT11GT13CA2CTTA4TA5TA28CACG4CG4CG1AG18AG1TG9AG5
D SRR765688.45263 IGHD2/OR15-2a*01 100.00 9 0 0 0 252 260 1 9 0.66 18.4 AGAATATTG AGAATATTG 9
D SRR765688.45263 IGHD2/OR15-2b*01 100.00 9 0 0 0 252 260 1 9 0.66 18.4 AGAATATTG AGAATATTG 9
D SRR765688.45263 IGHD5-24*01 100.00 7 0 0 0 264 270 3 9 11 14.4 AGAGATG AGAGATG 7
J SRR765688.45263 IGHJ3*02 96.00 50 2 0 0 269 318 1 50 1e-20 83.8 TGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAG TGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 15GC8CA25
J SRR765688.45263 IGHJ3*01 94.00 50 3 0 0 269 318 1 50 3e-18 75.8 TGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAG TGATGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 13AG1GC8CA25
J SRR765688.45263 IGHJ4*03 88.24 34 4 0 0 285 318 15 48 2e-06 36.2 TGGGGCCACGGGACAATGGTCACCGTCTCTTCAG TGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 8CA5ACAC13TC4
# IGBLASTN 2.2.29+
# Query: SRR765688.48840 HQP9GKT01BLP5E length=367|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-5*02 IGHD2-21*02,IGHD2-8*02,IGHD3-10*01 IGHJ3*01,IGHJ3*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGCA TGTATACTGGTTCGACGGG GAC (TGCT) TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 39 19 18 1 0 94.7
CDR1-IMGT 40 69 30 23 7 0 76.7
FR2-IMGT 70 120 51 45 6 0 88.2
CDR2-IMGT 121 141 21 18 3 0 85.7
FR3-IMGT 142 255 114 99 15 0 86.8
CDR3-IMGT (germline) 256 262 7 5 2 0 71.4
Total N/A N/A 242 208 34 0 86
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.48840 IGHV2-5*02 85.95 242 34 0 0 21 262 57 298 2e-75 272 GCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCA GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 13GT7CG9GACG4GA5AG1TG1CG10TA28AG7TCTA1ATTC2AT9AG4GT5GC9CT4TG4CG3AG6GA2GA16CA1GA12AT2GA3GA14TG1AG17TC5GA1GA2
V SRR765688.48840 IGHV2-5*01 85.54 242 35 0 0 21 262 57 298 2e-74 269 GCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCA GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGAATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 13GT7CG9GACG4GA5AG1TG1CG10TA28AG7TCTA1ATTC2AT6GA2AG4GT5GC9CT4TG4CG3AG6GA2GA16CA1GA12AT2GA3GA14TG1AG17TC5GA1GA2
V SRR765688.48840 IGHV2-5*05 85.54 242 35 0 0 21 262 57 298 2e-74 269 GCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCA GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACGGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACA 13GT7CG9GACG4GA5AG1TG1CG10TA28AG7TCTA1ATTC2AT9AG4GT5GC3AG5CT4TG4CG3AG6GA2GA16CA1GA12AT2GA3GA14TG1AG17TC5GA1GA2
D SRR765688.48840 IGHD2-21*02 100.00 7 0 0 0 282 288 17 23 11 14.4 GACTGCT GACTGCT 7
D SRR765688.48840 IGHD2-8*02 100.00 7 0 0 0 267 273 10 16 11 14.4 TACTGGT TACTGGT 7
D SRR765688.48840 IGHD3-10*01 100.00 7 0 0 0 270 276 10 16 11 14.4 TGGTTCG TGGTTCG 7
J SRR765688.48840 IGHJ3*01 89.36 47 5 0 0 285 331 4 50 1e-11 54.0 TGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAG TGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 10CG17GAAT4CAAC11
J SRR765688.48840 IGHJ3*02 89.36 47 5 0 0 285 331 4 50 1e-11 54.0 TGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAG TGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 10CA17GAAT4CAAC11
J SRR765688.48840 IGHJ6*02 100.00 16 0 0 0 296 311 28 43 4e-05 32.2 TCTGGGGCCAAGGGAC TCTGGGGCCAAGGGAC 16
# IGBLASTN 2.2.29+
# Query: SRR765688.36695 HQP9GKT01BVA49 length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGAG GTGGCTG AGTCGGCCGGCCAAG GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 40 2 0 95.2
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 47 4 0 92.2
CDR2-IMGT 138 161 24 24 0 0 100
FR3-IMGT 162 275 114 100 14 0 87.7
CDR3-IMGT (germline) 276 282 7 7 0 0 100
Total N/A N/A 262 236 26 0 90.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.36695 IGHV7-4-1*02 90.08 262 26 0 0 21 282 34 295 3e-92 329 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC13TA8AG11AG5TC10
V SRR765688.36695 IGHV7-4-1*04 89.69 262 27 0 0 21 282 34 295 2e-91 325 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAG 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC1CT10GC13TA8AG11AG5TC10
V SRR765688.36695 IGHV7-4-1*01 89.66 261 27 0 0 21 281 34 294 7e-91 324 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC5AT7TA8AG11AG5TC9
D SRR765688.36695 IGHD6-19*01 100.00 7 0 0 0 287 293 11 17 11 14.4 GTGGCTG GTGGCTG 7
D SRR765688.36695 IGHD5-12*01 100.00 6 0 0 0 287 292 10 15 46 12.4 GTGGCT GTGGCT 6
D SRR765688.36695 IGHD1-26*01 100.00 5 0 0 0 282 286 9 13 183 10.4 GGGAG GGGAG 5
J SRR765688.36695 IGHJ4*02 97.50 40 1 0 0 309 348 9 48 5e-17 71.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22AT17
J SRR765688.36695 IGHJ4*01 95.00 40 2 0 0 309 348 9 48 1e-14 63.9 GACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14GA7AT17
J SRR765688.36695 IGHJ5*02 97.14 35 1 0 0 314 348 17 51 5e-14 61.9 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.13959 HQP9GKT01AER44 length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR4|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV3-11*06 IGHD5-12*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) TGGCTAC AGATATACCCCA GGACG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 54 35 34 1 0 97.1
CDR1-IMGT 55 78 24 21 3 0 87.5
FR2-IMGT 79 129 51 51 0 0 100
CDR2-IMGT 130 153 24 20 4 0 83.3
FR3-IMGT 154 267 114 111 3 0 97.4
CDR3-IMGT (germline) 268 274 7 7 0 0 100
Total N/A N/A 255 244 11 0 95.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.13959 IGHV3-11*06 95.69 255 11 0 0 20 274 41 295 4e-103 364 CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAG CTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTAGTAGTAGTAGTTACACAAACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGAG 13AG28GC1CT13TC58CG2CGCT4CG17CA19GA73AG16
V SRR765688.13959 IGHV3-11*05 95.29 255 12 0 0 20 274 41 295 4e-102 361 CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAG CTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTAGTAGTAGTAGTTACACAAACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATTACTGTGCGAGAG 13AG28GC1CT13TC58CG2CGCT4CG17CA19GA70TC2AG16
V SRR765688.13959 IGHV3-11*03 95.28 254 12 0 0 20 273 41 294 1e-101 360 CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGA CTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTAGTAGTAGTAGTTACACAAACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATTACTGTGCGAGA 13AG28GC1CT13TC58CG2CGCT4CG17CA19GA70TC2AG15
D SRR765688.13959 IGHD5-12*01 100.00 9 0 0 0 273 281 9 17 0.69 18.4 AGTGGCTAC AGTGGCTAC 9
D SRR765688.13959 IGHD5-24*01 100.00 8 0 0 0 275 282 8 15 2.8 16.4 TGGCTACA TGGCTACA 8
D SRR765688.13959 IGHD6-19*01 100.00 7 0 0 0 273 279 10 16 11 14.4 AGTGGCT AGTGGCT 7
J SRR765688.13959 IGHJ6*02 97.50 40 1 0 0 294 333 23 62 5e-17 71.9 GGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCA GGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 19GA20
J SRR765688.13959 IGHJ6*01 95.12 41 2 0 0 294 334 23 63 3e-15 65.9 GGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCAG GGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 12CG6GA21
J SRR765688.13959 IGHJ6*04 95.12 41 2 0 0 294 334 23 63 3e-15 65.9 GGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCAG GGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 13CA5GA21
# IGBLASTN 2.2.29+
# Query: SRR765688.24180 HQP9GKT01CPLH4 length=353|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD2-8*01,IGHD2-8*02,IGHD5-24*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TTACAAT TATGC (TAT) GGACG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 22 8 0 73.3
FR2-IMGT 63 113 51 50 1 0 98
CDR2-IMGT 114 140 27 24 3 0 88.9
FR3-IMGT 141 254 114 106 8 0 93
CDR3-IMGT (germline) 255 262 8 8 0 0 100
Total N/A N/A 243 223 20 0 91.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.24180 IGHV6-1*01 91.77 243 20 0 0 20 262 63 305 5e-89 318 CTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 15CG4CG1AG6AGTCGA4ATAG10TC66GTGA2GA12CA17GA2TC6GC32TC4TC2AT17TG23
V SRR765688.24180 IGHV6-1*02 91.77 243 20 0 0 20 262 63 305 5e-89 318 CTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 15CG4CG1AG6AGTCGA4ATAG10TC66GTGA2GA12CA17GA2TC6GC32TC4TC2AT17TG23
V SRR765688.24180 IGHV4-30-4*01 68.50 200 57 1 6 63 262 106 299 1e-26 110 TGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGA TGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTG------GGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCAGACACGGCCGTGTATTACTGTGCCAGAGA 4AG7AC1GC3TC5TGCG2GAAG5TG6CA1GT2AGATGAGC1CTAC2CT5GTTGC-C-A-A-G-T-2GA1TC1GCTCGT1TC2TCGACAACGCTCCG2TCGC1GC2AG6AG1GT2TC2CAATACCA1CT6AG21CA2TC3AGTC2AT6CG3ACGA5TG2TC14AC5
D SRR765688.24180 IGHD2-8*01 100.00 8 0 0 0 270 277 21 28 2.6 16.4 TATGCTAT TATGCTAT 8
D SRR765688.24180 IGHD2-8*02 100.00 8 0 0 0 270 277 21 28 2.6 16.4 TATGCTAT TATGCTAT 8
D SRR765688.24180 IGHD5-24*01 100.00 8 0 0 0 264 271 12 19 2.6 16.4 TACAATTA TACAATTA 8
J SRR765688.24180 IGHJ6*02 100.00 43 0 0 0 275 317 20 62 3e-21 85.7 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 43
J SRR765688.24180 IGHJ6*01 97.73 44 1 0 0 275 318 20 63 2e-19 79.8 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 15CG28
J SRR765688.24180 IGHJ6*04 97.73 44 1 0 0 275 318 20 63 2e-19 79.8 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 16CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.43187 HQP9GKT01C588C length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD3-22*01 IGHJ6*02,IGHJ6*04 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA TCCCGCCGAC TATTACTATGATAATAGTGGT CACCTAATT TACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 29 1 0 96.7
FR2-IMGT 63 113 51 50 1 0 98
CDR2-IMGT 114 140 27 27 0 0 100
FR3-IMGT 141 254 114 110 4 0 96.5
CDR3-IMGT (germline) 255 262 8 7 1 0 87.5
Total N/A N/A 243 236 7 0 97.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.43187 IGHV6-1*01 97.12 243 7 0 0 20 262 63 305 3e-101 358 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 35TG11GA84TA8GA9GA61CG24CA4
V SRR765688.43187 IGHV6-1*02 97.12 243 7 0 0 20 262 63 305 3e-101 358 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 35TG11GA84TA8GA9GA61CG24CA4
V SRR765688.43187 IGHV4-30-2*03 70.62 194 51 1 6 63 256 106 293 2e-29 120 TGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGC TGGAGCTGGATCCGGCAGCCACCAGGGAAGGGCCTGGAGTGGATTGGGAGTATCTATTATAGTG------GGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCAGACACGGCTGTGTATTACTGTGC 12AC5TC1CA3TGCG2GAAG5TG6CA1GT2AG2GT1CTAC2CT2CT2GTTGC-C-A-A-G-T-2TAAGTC1ACTCGT1TC2TCGACAACGCTCTG2TCGC1GC9AG1ACGA4CAATAC1CGCT6AG21CA6AG9TCCG1CT1ACCA23
D SRR765688.43187 IGHD3-22*01 95.24 21 1 0 0 273 293 2 22 4e-05 32.4 TATTACTATGATAATAGTGGT TATTACTATGATAGTAGTGGT 13AG7
D SRR765688.43187 IGHD3-10*01 100.00 10 0 0 0 273 282 2 11 0.17 20.4 TATTACTATG TATTACTATG 10
D SRR765688.43187 IGHD3-10*02 100.00 10 0 0 0 273 282 2 11 0.17 20.4 TATTACTATG TATTACTATG 10
J SRR765688.43187 IGHJ6*02 100.00 30 0 0 0 303 332 6 35 2e-13 60.0 TACTACTACTACGGTATGGACGTCTGGGGC TACTACTACTACGGTATGGACGTCTGGGGC 30
J SRR765688.43187 IGHJ6*04 100.00 30 0 0 0 303 332 6 35 2e-13 60.0 TACTACTACTACGGTATGGACGTCTGGGGC TACTACTACTACGGTATGGACGTCTGGGGC 30
J SRR765688.43187 IGHJ6*01 100.00 29 0 0 0 303 331 6 34 7e-13 58.0 TACTACTACTACGGTATGGACGTCTGGGG TACTACTACTACGGTATGGACGTCTGGGG 29
# IGBLASTN 2.2.29+
# Query: SRR765688.46667 HQP9GKT01COT4Z length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR10|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-31*03 IGHD4-23*01,IGHD4-17*01 IGHJ1*01 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TGCGA C CTACGGT CGCAGTCGCGAGA CTGAA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 19 54 36 35 1 0 97.2
CDR1-IMGT 55 84 30 26 4 0 86.7
FR2-IMGT 85 135 51 49 2 0 96.1
CDR2-IMGT 136 156 21 19 2 0 90.5
FR3-IMGT 157 270 114 100 14 0 87.7
CDR3-IMGT (germline) 271 274 4 4 0 0 100
Total N/A N/A 256 233 23 0 91
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46667 IGHV4-31*03 91.02 256 23 0 0 19 274 40 295 3e-92 329 CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGA CCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGA 28GC16CA3CG5TG2AG16CA40TC11CT6GA3GAGC14GC9AG2TA2TA7CA17AG10TAAGGC3AG27CG5TC7
V SRR765688.46667 IGHV4-31*04 90.98 255 23 0 0 19 273 40 294 8e-92 327 CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCG CCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCG 28GC16CA3CG5TG2AG16CA40TC11CT6GA3GAGC14GC9AG2TA2TA7CA17AG10TAAGGC3AG27CG5TC6
V SRR765688.46667 IGHV4-31*01 90.62 256 24 0 0 19 274 40 295 2e-91 325 CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGA CCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCTAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGA 28GC16CA3CG5TG2AG16CA40TC11CT6GA3GAGC14GC7GT1AG2TA2TA7CA17AG10TAAGGC3AG27CG5TC7
D SRR765688.46667 IGHD4-23*01 100.00 7 0 0 0 276 282 4 10 11 14.4 CTACGGT CTACGGT 7
D SRR765688.46667 IGHD4-17*01 100.00 7 0 0 0 276 282 4 10 11 14.4 CTACGGT CTACGGT 7
D SRR765688.46667 IGHD5-12*01 100.00 5 0 0 0 276 280 14 18 181 10.4 CTACG CTACG 5
J SRR765688.46667 IGHJ1*01 98.04 51 1 0 0 296 346 2 52 1e-23 93.7 CTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG CTGAATACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 14TC36
J SRR765688.46667 IGHJ4*02 97.30 37 1 0 0 310 346 12 48 3e-15 65.9 TACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG TACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 14CA22
J SRR765688.46667 IGHJ5*02 97.14 35 1 0 0 312 346 17 51 5e-14 61.9 CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 12CA22
# IGBLASTN 2.2.29+
# Query: SRR765688.38963 HQP9GKT01C40UI length=376|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-70*11 IGHD3-3*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GGTTA AACCCTG ACGATTTCTGGAGTGG CACCT ACTAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 39 19 19 0 0 100
CDR1-IMGT 40 69 30 26 4 0 86.7
FR2-IMGT 70 120 51 50 1 0 98
CDR2-IMGT 121 141 21 21 0 0 100
FR3-IMGT 142 255 114 103 11 0 90.4
CDR3-IMGT (germline) 256 264 9 8 1 0 88.9
Total N/A N/A 244 227 17 0 93
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.38963 IGHV2-70*11 93.03 244 17 0 0 21 264 57 300 3e-92 329 ACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTA ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGATA 21AG11TC1GC10AT5GA74CA1TC14GC2AG20TC1TC2GA15CT29CA8CT1TA10TA2
V SRR765688.38963 IGHV2-70*01 92.62 244 18 0 0 21 264 57 300 2e-91 325 ACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTA ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGTGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACTCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGATA 21AG11TC1GC10AT5GA45GT28CA1TC14GC2AG20TC1TC2GA15CT29CA8CT1TA10TA2
V SRR765688.38963 IGHV2-70*10 92.62 244 18 0 0 21 264 57 300 2e-91 325 ACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTA ACTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGCGTGTGAGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGATTGCACGCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACGGATA 21AG11TC1GC10AC5GA38CA35CA1TC14GC2AG20TC1TC2GA15CT29CA8CT1TA10TA2
D SRR765688.38963 IGHD3-3*01 93.75 16 1 0 0 272 287 6 21 0.044 22.4 ACGATTTCTGGAGTGG ACGATTTTTGGAGTGG 7CT8
D SRR765688.38963 IGHD3-3*02 100.00 8 0 0 0 280 287 14 21 2.8 16.4 TGGAGTGG TGGAGTGG 8
D SRR765688.38963 IGHD5-12*01 100.00 6 0 0 0 272 277 16 21 45 12.4 ACGATT ACGATT 6
J SRR765688.38963 IGHJ4*02 97.92 48 1 0 0 293 340 1 48 8e-22 87.7 ACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 11CT36
J SRR765688.38963 IGHJ4*01 95.83 48 2 0 0 293 340 1 48 2e-19 79.8 ACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 11CT10GA25
J SRR765688.38963 IGHJ5*02 97.50 40 1 0 0 301 340 12 51 5e-17 71.9 GACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4AC35
# IGBLASTN 2.2.29+
# Query: SRR765688.36155 HQP9GKT01BS5TB length=362|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD6-6*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG CGAAA TATAGCAGCTCGTC AA ACTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 27 3 0 90
FR2-IMGT 63 113 51 49 2 0 96.1
CDR2-IMGT 114 140 27 23 4 0 85.2
FR3-IMGT 141 254 114 96 18 0 84.2
CDR3-IMGT (germline) 255 261 7 7 0 0 100
Total N/A N/A 242 215 27 0 88.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.36155 IGHV6-1*01 88.84 242 27 0 0 20 261 63 304 5e-82 294 CTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 14AG13GA9GC9TC32AG13TCTA1TA19TA4AT6CGCTCA9GA8GATC6TC14AG5AG11AG4CAGC2CT1CT9CG11TG5TC10
V SRR765688.36155 IGHV6-1*02 88.84 242 27 0 0 20 261 63 304 5e-82 294 CTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 14AG13GA9GC9TC32AG13TCTA1TA19TA4AT6CGCTCA9GA8GATC6TC14AG5AG11AG4CAGC2CT1CT9CG11TG5TC10
V SRR765688.36155 IGHV4-30-4*01 64.04 228 70 2 12 40 261 77 298 4e-20 89.1 GTGTCTCTGGCAACAGTGGTGCTT------GGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAG GTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATTACAGTGGGAGCACCTACTACAAC------CCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCAGACACGGCCGTGTATTACTGTGCCAGAG 3TG3TCGAGT2AG8CA2-A-C-T-A-C-T3AG7AC1GC3TC5TGCG2GAAG5TG2AG3CA1GT2AGATGAGC2TC1TACT5GTTGCGCG1AGGCTAGCGC2TC2TCGA1ACT-A-T-G-C-A-2CG2TCGC1GC2AGGA5AG1ATGATC3CAATACTACGCT6AG5AG5AG9CA1AG4CGGC2CT1CT4CG3ACCA8TC2TG5TC5AC4
D SRR765688.36155 IGHD6-6*01 100.00 14 0 0 0 267 280 4 17 7e-04 28.4 TATAGCAGCTCGTC TATAGCAGCTCGTC 14
D SRR765688.36155 IGHD6-13*01 100.00 9 0 0 0 267 275 4 12 0.68 18.4 TATAGCAGC TATAGCAGC 9
D SRR765688.36155 IGHD6-25*01 100.00 9 0 0 0 267 275 4 12 0.68 18.4 TATAGCAGC TATAGCAGC 9
J SRR765688.36155 IGHJ4*02 97.78 45 1 0 0 283 327 4 48 5e-20 81.8 ACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 9TA35
J SRR765688.36155 IGHJ4*01 95.56 45 2 0 0 283 327 4 48 1e-17 73.8 ACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 9TA9GA25
J SRR765688.36155 IGHJ5*02 100.00 35 0 0 0 293 327 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.18975 HQP9GKT01BH3GZ length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01 IGHD3-3*01,IGHD3-3*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GAAGTTACAATTA TTGGAGTGATTATT GTTCCCACC TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 23 3 0 88.5
CDR1-IMGT 47 70 24 22 2 0 91.7
FR2-IMGT 71 121 51 47 4 0 92.2
CDR2-IMGT 122 142 21 19 2 0 90.5
FR3-IMGT 143 256 114 105 9 0 92.1
CDR3-IMGT (germline) 257 263 7 7 0 0 100
Total N/A N/A 243 223 20 0 91.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.18975 IGHV4-59*01 91.77 243 20 0 0 21 263 50 292 5e-89 318 CCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 17TA1CTAG20CACG18TC6TC8CA10AG27CA1TC3GACATC4GA27GA3CG19AG11CG3GA45
V SRR765688.18975 IGHV4-59*07 91.74 242 20 0 0 21 262 50 291 2e-88 316 CCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 17TA1CTAG20CACG18TC6TC8CA10AG27CA1TC3GACATC4GA27GA3CG19AG11CG3GA44
V SRR765688.18975 IGHV4-59*03 92.05 239 19 0 0 21 259 50 288 4e-88 315 CCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCG 17TA1CTAG20CACG18TC6TC8CA10AG27CA1TC3GACATC4GA27GA3CG31CG3GA41
D SRR765688.18975 IGHD3-3*01 92.86 14 1 0 0 277 290 13 26 0.71 18.4 TTGGAGTGATTATT TTGGAGTGGTTATT 8AG5
D SRR765688.18975 IGHD3-3*02 92.86 14 1 0 0 277 290 13 26 0.71 18.4 TTGGAGTGATTATT TTGGAGTGGTTATT 8AG5
D SRR765688.18975 IGHD5-24*01 100.00 8 0 0 0 269 276 12 19 2.8 16.4 TACAATTA TACAATTA 8
J SRR765688.18975 IGHJ4*02 97.62 42 1 0 0 300 341 7 48 3e-18 75.8 TTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 19CA22
J SRR765688.18975 IGHJ1*01 100.00 36 0 0 0 306 341 17 52 5e-17 71.9 ACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG ACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 36
J SRR765688.18975 IGHJ4*01 95.24 42 2 0 0 300 341 7 48 7e-16 67.9 TTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 16GA2CA22
# IGBLASTN 2.2.29+
# Query: SRR765688.46037 HQP9GKT01APHCO length=359|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD6-19*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG GTTATG GCAGTGGCTGGTAC TGGATAA CCTGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 23 7 0 76.7
FR2-IMGT 63 113 51 49 2 0 96.1
CDR2-IMGT 114 140 27 20 7 0 74.1
FR3-IMGT 141 254 114 106 8 0 93
CDR3-IMGT (germline) 255 261 7 6 1 0 85.7
Total N/A N/A 242 217 25 0 89.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46037 IGHV6-1*01 89.67 242 25 0 0 20 261 63 304 7e-84 300 CTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 15AG1TA1GAAG8CG1TA8TG11GA37CA5TA2TC4AGTG3CA5ATTA15GA8GA32AG6GT10CG5TC14CG3GA18TC5
V SRR765688.46037 IGHV6-1*02 89.67 242 25 0 0 20 261 63 304 7e-84 300 CTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 15AG1TA1GAAG8CG1TA8TG11GA37CA5TA2TC4AGTG3CA5ATTA15GA8GA32AG6GT10CG5TC14CG3GA18TC5
V SRR765688.46037 IGHV4-30-2*03 68.66 201 57 1 6 60 260 103 297 4e-27 112 TCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGA TCCTGGAGCTGGATCCGGCAGCCACCAGGGAAGGGCCTGGAGTGGATTGGGAGTATCTATTATAGTG------GGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCAGACACGGCTGTGTATTACTGTGCGAGA 2TC4AG4GA2AC5TC1CA3TGCG2GAAG5TG6CA1GT2CG2GT1CTTC5CT1AG1TGC-C-C-A-G-T-3TGTC1ACTCGT1TC2TCGACAACGCTC3TCGC1GC9AG1AC5CAATAC1CGCT6AG5AG6GT8CA1CG4AGTC8TCCG1CT1ACCA3GA18TCAG3
D SRR765688.46037 IGHD6-19*01 100.00 14 0 0 0 268 281 8 21 7e-04 28.4 GCAGTGGCTGGTAC GCAGTGGCTGGTAC 14
D SRR765688.46037 IGHD6-13*01 100.00 8 0 0 0 274 281 14 21 2.7 16.4 GCTGGTAC GCTGGTAC 8
D SRR765688.46037 IGHD2-8*02 100.00 7 0 0 0 278 284 9 15 11 14.4 GTACTGG GTACTGG 7
J SRR765688.46037 IGHJ5*02 94.44 36 2 0 0 289 324 16 51 3e-12 56.0 CCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG CCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 19AG8GC7
J SRR765688.46037 IGHJ4*02 94.29 35 2 0 0 290 324 14 48 1e-11 54.0 CTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 18AG8GC7
J SRR765688.46037 IGHJ5*01 91.67 36 3 0 0 289 324 16 51 6e-10 48.1 CCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG CCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 10GA8AG8GC7
# IGBLASTN 2.2.29+
# Query: SRR765688.15636 HQP9GKT01ATB6I length=377|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR8|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-5*02 IGHD2-21*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CTCAG TCGCCGTCT TGGTGACT CCGGAGTGCAGAAAT ACTAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 39 19 19 0 0 100
CDR1-IMGT 40 69 30 22 8 0 73.3
FR2-IMGT 70 121 52 49 2 1 94.2
CDR2-IMGT 122 142 21 15 6 0 71.4
FR3-IMGT 143 256 114 100 14 0 87.7
CDR3-IMGT (germline) 257 264 8 5 3 0 62.5
Total N/A N/A 244 210 33 1 86.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.15636 IGHV2-5*02 86.07 244 33 1 1 21 264 57 299 2e-74 269 GCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCA-GGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 28AC2GAAGACGA2GAAG8CG24CAT-3GA23TAATCT1TA14GAAGTCAG5CG1TC7CG5GC8TC5TC13GA6CG3AG6GCGA19CT23TCCA1TA3
V SRR765688.15636 IGHV2-5*01 85.66 244 34 1 1 21 264 57 299 2e-73 266 GCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCA-GGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGAATGATGATAAGCGCTACAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 28AC2GAAGACGA2GAAG8CG24CAT-3GA23TAATCT1TA4GA9GAAGTCAG5CG1TC7CG5GC8TC5TC13GA6CG3AG6GCGA19CT23TCCA1TA3
V SRR765688.15636 IGHV2-5*05 85.66 244 34 1 1 21 264 57 299 2e-73 266 GCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAG GCTGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCA-GGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACGGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCACACAG 28AC2GAAGACGA2GAAG8CG24CAT-3GA23TAATCT1TA14GAAGTCAG4AGCG1TC7CG5GC8TC5TC13GA6CG3AG6GCGA19CT23TCCA1TA3
D SRR765688.15636 IGHD2-21*02 100.00 8 0 0 0 274 281 13 20 2.8 16.4 TGGTGACT TGGTGACT 8
D SRR765688.15636 IGHD4-17*01 100.00 7 0 0 0 275 281 8 14 11 14.4 GGTGACT GGTGACT 7
D SRR765688.15636 IGHD2-21*01 100.00 6 0 0 0 274 279 13 18 45 12.4 TGGTGA TGGTGA 6
J SRR765688.15636 IGHJ4*02 97.92 48 1 0 0 297 344 1 48 8e-22 87.7 ACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG ACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 26GA21
J SRR765688.15636 IGHJ4*01 95.83 48 2 0 0 297 344 1 48 2e-19 79.8 ACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG ACTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 22GA3GA21
J SRR765688.15636 IGHJ4*03 93.62 47 3 0 0 298 344 2 48 2e-16 69.9 CTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG CTACTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 21GA2AGGA21
# IGBLASTN 2.2.29+
# Query: SRR765688.40959 HQP9GKT01BMXS4 length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*08 IGHD3-16*01,IGHD3-16*02,IGHD5-24*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGCGA (GA) GTT CAATTTCCTAGGTT CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 70 24 23 1 0 95.8
FR2-IMGT 71 121 51 45 6 0 88.2
CDR2-IMGT 122 142 21 15 6 0 71.4
FR3-IMGT 143 256 114 98 16 0 86
CDR3-IMGT (germline) 257 262 6 6 0 0 100
Total N/A N/A 242 212 30 0 87.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.40959 IGHV4-59*08 87.60 242 30 0 0 21 262 50 291 3e-79 285 CCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGA 17TA23GA12CG13TC13CATC13AGCT2GA2CT3CATCGA4AG14TC10GA10GAAC1TA7CA17CG8AG1GAAGAC3CG3CT23CG5TC2CT6
V SRR765688.40959 IGHV4-4*08 87.24 243 31 0 0 21 263 50 292 1e-78 283 CCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGAG 17TA23GA12CG13TC13CATC13AGCT2GA2CT2TA1TCGA4AG14TC10GA10GAAC1TA4AC2CA17CG8AG1GAAGAC3CG3CT23CG5TC2CT7
V SRR765688.40959 IGHV4-59*01 86.83 243 32 0 0 21 263 50 292 9e-78 280 CCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 17TA23GA12CG13TC13CATC13AGCT2GA2CT3CATCGA4AG14TC10GA10GAAC1TA7CA17CG8AG1GAAGAC3CG3CT8CT2AG11CG5TC2CT7
D SRR765688.40959 IGHD3-16*01 100.00 5 0 0 0 261 265 22 26 171 10.4 GAGTT GAGTT 5
D SRR765688.40959 IGHD3-16*02 100.00 5 0 0 0 261 265 22 26 171 10.4 GAGTT GAGTT 5
D SRR765688.40959 IGHD5-24*01 100.00 5 0 0 0 266 270 14 18 171 10.4 CAATT CAATT 5
J SRR765688.40959 IGHJ4*02 89.36 47 5 0 0 280 326 2 48 1e-11 54.0 CTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAG CTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 12TC2CG10TCTC1AT17
J SRR765688.40959 IGHJ4*01 87.23 47 6 0 0 280 326 2 48 3e-09 46.1 CTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAG CTACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 12TC2CG5GA4TCTC1AT17
J SRR765688.40959 IGHJ5*02 90.32 31 3 0 0 296 326 21 51 6e-07 38.2 GGCCAGGGAATTCAGGTCACCGTCTCCTCAG GGCCAGGGAACCCTGGTCACCGTCTCCTCAG 10TCTC1AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.44149 HQP9GKT01CLVEF length=360|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-26*01 IGHJ6*01 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG (A) TAGTGGG N/A GGTAT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 42 0 0 100
CDR1-IMGT 63 86 24 23 1 0 95.8
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 114 0 0 100
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 257 6 0 97.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.44149 IGHV7-4-1*02 97.72 263 6 0 0 21 283 34 296 1e-111 392 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 63TG5CA49TC3TATC11CA126
V SRR765688.44149 IGHV7-4-1*04 97.34 263 7 0 0 21 283 34 296 1e-110 389 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 63TG5CA49TC3TATC11CA62CT63
V SRR765688.44149 IGHV7-4-1*01 97.32 261 7 0 0 21 281 34 294 1e-109 386 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 63TG5CA49TC3TATC11CA79AT44
D SRR765688.44149 IGHD1-26*01 100.00 8 0 0 0 283 290 4 11 2.5 16.4 ATAGTGGG ATAGTGGG 8
D SRR765688.44149 IGHD5-12*01 100.00 7 0 0 0 283 289 7 13 9.9 14.4 ATAGTGG ATAGTGG 7
D SRR765688.44149 IGHD2-15*01 100.00 6 0 0 0 284 289 10 15 40 12.4 TAGTGG TAGTGG 6
J SRR765688.44149 IGHJ6*01 100.00 19 0 0 0 291 309 18 36 6e-07 38.2 GGTATGGACGTCTGGGGGC GGTATGGACGTCTGGGGGC 19
J SRR765688.44149 IGHJ6*02 100.00 17 0 0 0 291 307 18 34 9e-06 34.2 GGTATGGACGTCTGGGG GGTATGGACGTCTGGGG 17
J SRR765688.44149 IGHJ6*04 100.00 17 0 0 0 291 307 18 34 9e-06 34.2 GGTATGGACGTCTGGGG GGTATGGACGTCTGGGG 17
# IGBLASTN 2.2.29+
# Query: SRR765688.9210 HQP9GKT01ED1VA length=368|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD1/OR15-1a*01,IGHD1/OR15-1b*01,IGHD6-19*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAGAG GTCGC GAACAA GATCTTCCGTCTGCTGACTTTAACCAG TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 24 6 0 80
FR2-IMGT 63 113 51 49 2 0 96.1
CDR2-IMGT 114 140 27 22 5 0 81.5
FR3-IMGT 141 254 114 91 23 0 79.8
CDR3-IMGT (germline) 255 261 7 7 0 0 100
Total N/A N/A 242 206 36 0 85.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.9210 IGHV6-1*01 85.12 242 36 0 0 20 261 63 304 1e-73 266 CTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 28CA3GA2TG2TC1AG1CT17AG5GA28TCCA13GA5TA1TA4GT3TG4GA3CG1AGCA2GA7GACAGC1TA2TA3AC18AGCT10AGTC4TC1TC5GC6AG11CG4TA11
V SRR765688.9210 IGHV6-1*02 85.12 242 36 0 0 20 261 63 304 1e-73 266 CTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAG CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAG 28CA3GA2TG2TC1AG1CT17AG5GA28TCCA13GA5TA1TA4GT3TG4GA3CG1AGCA2GA7GACAGC1TA2TA3AC18AGCT10AGTC4TC1TC5GC6AG11CG4TA11
V SRR765688.9210 IGHV4-30-2*03 65.50 200 63 1 6 61 260 104 297 5e-21 92.2 CCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGA CCTGGAGCTGGATCCGGCAGCCACCAGGGAAGGGCCTGGAGTGGATTGGGAGTATCTATTATAGTGGGAGCACCTACTACAACCC------GTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCAGACACGGCTGTGTATTACTGTGCGAGA 6AG7AC4AGTC1CA2GATGCG2GAAG5TG6CA1GT2AG2GT5CT2CT2GTTGCGCG2GCTAGCGC1TATC2TCGA1GCTCACT-T-C-A-G-T-3TC2ACCA1AGGA5AG1GCCAGC1TA1CA1AC1CGAT6AG11AGCT8CA1AGTC3AGTC1TC5GCTCCG1CT1AC12CG4TA6AG3
D SRR765688.9210 IGHD1/OR15-1a*01 100.00 6 0 0 0 267 272 11 16 44 12.4 GAACAA GAACAA 6
D SRR765688.9210 IGHD1/OR15-1b*01 100.00 6 0 0 0 267 272 11 16 44 12.4 GAACAA GAACAA 6
D SRR765688.9210 IGHD6-19*01 100.00 6 0 0 0 297 302 9 14 44 12.4 CAGTGG CAGTGG 6
J SRR765688.9210 IGHJ4*02 91.18 34 3 0 0 300 333 15 48 1e-08 44.1 TGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 6AC6TC9TC10
J SRR765688.9210 IGHJ5*02 91.18 34 3 0 0 300 333 18 51 1e-08 44.1 TGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 6AC6TC9TC10
J SRR765688.9210 IGHJ1*01 88.24 34 4 0 0 300 333 19 52 2e-06 36.2 TGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG TGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 6AC4AC1TC9TC10
# IGBLASTN 2.2.29+
# Query: SRR765688.31439 HQP9GKT01BFFKN length=371|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA TTCCGGGCCTAAC AGTGTCT TGTT CTGGT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 20 4 0 83.3
FR2-IMGT 71 121 51 48 3 0 94.1
CDR2-IMGT 122 142 21 17 4 0 81
FR3-IMGT 143 256 114 102 12 0 89.5
CDR3-IMGT (germline) 257 264 8 8 0 0 100
Total N/A N/A 244 221 23 0 90.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.31439 IGHV4-59*08 90.57 244 23 0 0 21 264 50 293 1e-86 310 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC37CTGA14TC5TC7GA5AGTC3CG25CT1TA12
V SRR765688.31439 IGHV4-4*08 89.67 242 25 0 0 21 262 50 291 7e-84 300 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GAAC2TG2AG2CG4TA1TC35AC1CTGA14TC5TC7GA5AGTC3CG25CT1TA10
V SRR765688.31439 IGHV4-59*01 89.67 242 25 0 0 21 262 50 291 7e-84 300 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC37CTGA14TC5TC7GA5AGTC3CG7CT2AG14CT1TA10
D SRR765688.31439 IGHD5/OR15-5a*01 100.00 7 0 0 0 278 284 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.31439 IGHD5/OR15-5b*01 100.00 7 0 0 0 278 284 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.31439 IGHD2-21*01 100.00 5 0 0 0 264 268 24 28 176 10.4 ATTCC ATTCC 5
J SRR765688.31439 IGHJ5*02 100.00 47 0 0 0 289 335 5 51 1e-23 93.7 CTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 47
J SRR765688.31439 IGHJ5*01 95.74 47 2 0 0 289 335 5 51 7e-19 77.8 CTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 10CT10GA25
J SRR765688.31439 IGHJ4*02 100.00 35 0 0 0 301 335 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.33456 HQP9GKT01BSJ79 length=384|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-18*04 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA TCGAG GTAGTACCAG GTGGCTCGAACCCA TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 38 1 0 97.4
CDR1-IMGT 60 83 24 23 1 0 95.8
FR2-IMGT 84 134 51 50 1 0 98
CDR2-IMGT 135 158 24 23 1 0 95.8
FR3-IMGT 159 272 114 107 7 0 93.9
CDR3-IMGT (germline) 273 280 8 8 0 0 100
Total N/A N/A 260 249 11 0 95.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.33456 IGHV1-18*04 95.77 260 11 0 0 21 280 37 296 2e-105 372 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGAGA 31CA24TC52CG22GA7GC11AGTC32CT36TG18AG5TC11
V SRR765688.33456 IGHV1-18*01 95.38 260 12 0 0 21 280 37 296 2e-104 369 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGAGA 31CA24TC2CT49CG22GA7GC11AGTC32CT36TG18AG5TC11
V SRR765688.33456 IGHV1-18*03 95.00 260 13 0 0 21 280 37 296 2e-103 366 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGA AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAGCTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGTAACACAAACTATGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACATGGCCGTGTATTACTGTGCGAGAGA 31CA24TC2CT49CG22GA7GC11AGTC32CT36TG11CT6AG5TC11
D SRR765688.33456 IGHD2-2*01 100.00 10 0 0 0 286 295 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
D SRR765688.33456 IGHD2-2*02 100.00 10 0 0 0 286 295 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
D SRR765688.33456 IGHD2-2*03 100.00 10 0 0 0 286 295 12 21 0.18 20.4 GTAGTACCAG GTAGTACCAG 10
J SRR765688.33456 IGHJ4*02 100.00 42 0 0 0 310 351 7 48 1e-20 83.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 42
J SRR765688.33456 IGHJ4*01 97.62 42 1 0 0 310 351 7 48 3e-18 75.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 16GA25
J SRR765688.33456 IGHJ5*02 100.00 35 0 0 0 317 351 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.40824 HQP9GKT01B3H0W length=380|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*06,IGHV1-69*14 IGHD1-26*01 IGHJ3*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GCAT TGGGAGCTACTAC CGTGATGAC GCTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 18 6 0 75
FR2-IMGT 80 130 51 45 6 0 88.2
CDR2-IMGT 131 154 24 21 3 0 87.5
FR3-IMGT 155 268 114 102 12 0 89.5
CDR3-IMGT (germline) 269 275 7 7 0 0 100
Total N/A N/A 255 226 29 0 88.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.40824 IGHV1-69*06 88.63 255 29 0 0 21 275 41 295 3e-86 308 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAG CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 29AG2CT11CT2CAAG2AGAC4GC1CA3CG13AGTC6CA5AG22GT4CACT13GA14CT16CG2GT3AG1AG4CA24AG13CG2TC13AG7TC10
V SRR765688.40824 IGHV1-69*14 88.63 255 29 0 0 21 275 41 295 3e-86 308 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAG CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 29AG2CT11CT2CAAG2AGAC4GC1CA3CG13AGTC6CA5AG22GT4CACT13GA14CT16CG2GT3AG1AG4CA24AG13CG2TC13AG7TC10
V SRR765688.40824 IGHV1-69*05 88.58 254 29 0 0 21 274 41 294 1e-85 307 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGA CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCACGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGA 29AG2CT11CT2CAAG2AGAC4GC1CA3CG13AGTC6CA5AG22GT4CACT13GA14CT16CG2GT5AG3AGCA24AG13CG2TC13AG7TC9
D SRR765688.40824 IGHD1-26*01 100.00 13 0 0 0 280 292 8 20 0.003 26.4 TGGGAGCTACTAC TGGGAGCTACTAC 13
D SRR765688.40824 IGHD2-15*01 100.00 6 0 0 0 285 290 24 29 46 12.4 GCTACT GCTACT 6
D SRR765688.40824 IGHD2/OR15-2a*01 100.00 6 0 0 0 287 292 16 21 46 12.4 TACTAC TACTAC 6
J SRR765688.40824 IGHJ3*02 93.48 46 3 0 0 302 347 5 50 7e-16 67.9 GCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAG GCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 11GC15CA2AG15
J SRR765688.40824 IGHJ3*01 91.30 46 4 0 0 302 347 5 50 2e-13 60.0 GCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAG GCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 9AG1GC15CA2AG15
J SRR765688.40824 IGHJ4*03 91.18 34 3 0 0 314 347 15 48 1e-08 44.1 TGGGGCCAAGGGACACTGATCACCGTCTCTTCAG TGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 14AC3AG10TC4
# IGBLASTN 2.2.29+
# Query: SRR765688.25750 HQP9GKT01CM4A4 length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-34*01,IGHV4-34*02 IGHD6-19*01 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG ATCCTTAC AGCAGTGGCTGG CACGCCC ACTGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 70 24 20 4 0 83.3
FR2-IMGT 71 122 52 49 2 1 94.2
CDR2-IMGT 123 143 21 17 4 0 81
FR3-IMGT 144 257 114 103 11 0 90.4
CDR3-IMGT (germline) 258 264 7 6 1 0 85.7
Total N/A N/A 244 220 23 1 90.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.25750 IGHV4-34*01 90.16 244 23 1 1 21 264 50 292 7e-84 300 CCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAG-CCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAGAG 23CT12CTTC5GT1CA18TC3A-14AG20GC2GTGC11CA24CG4CG11AG18TATG11AG5GC5CG2TC13GC3TG10TC5
V SRR765688.25750 IGHV4-34*02 90.16 244 23 1 1 21 264 50 292 7e-84 300 CCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAG-CCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAGAG 23CT12CTTC5GT1CA18TC3A-14AG20GC2GTGC11CA24CG4CG11AG18TATG11AG5GC5CG2TC13GC3TG10TC5
V SRR765688.25750 IGHV4-34*09 89.80 245 24 1 1 21 265 50 293 2e-83 299 CCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAGA CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAG-CCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTTACCATATCAGTAGACACGTCTAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCGGACACGGCCGTGTATTACTGTGCGAGAGA 23CT12CTTC5GT1CA18TC3A-35GC2GTGC11CA24CG4CG1CT9AG10CT7TATG11AG5GC5CG16GCTC2TG10TC6
D SRR765688.25750 IGHD6-19*01 100.00 12 0 0 0 273 284 7 18 0.011 24.4 AGCAGTGGCTGG AGCAGTGGCTGG 12
D SRR765688.25750 IGHD5-12*01 100.00 7 0 0 0 276 282 9 15 11 14.4 AGTGGCT AGTGGCT 7
D SRR765688.25750 IGHD5-18*01 100.00 6 0 0 0 270 275 6 11 43 12.4 TACAGC TACAGC 6
J SRR765688.25750 IGHJ4*02 100.00 36 0 0 0 292 327 13 48 4e-17 71.9 ACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 36
J SRR765688.25750 IGHJ5*02 100.00 35 0 0 0 293 327 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.25750 IGHJ1*01 97.30 37 1 0 0 291 327 16 52 3e-15 65.9 CACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 14AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.49935 HQP9GKT01BCQ7U length=391|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgM-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD7-27*01 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CAAGA AGTAAGAGGT CTAACTGGGGA TCAGACTT CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 13 0 0 100
CDR1-IMGT 33 62 30 30 0 0 100
FR2-IMGT 63 112 51 49 1 1 96.1
CDR2-IMGT 113 139 27 26 1 0 96.3
FR3-IMGT 140 253 114 108 6 0 94.7
CDR3-IMGT (germline) 254 259 6 6 0 0 100
Total N/A N/A 241 232 8 1 96.3
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.49935 IGHV6-1*01 96.27 241 8 1 1 20 259 63 303 2e-96 343 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA 52GA36-G19CA11AGCA9CA9GA14GA34TG48
V SRR765688.49935 IGHV6-1*02 96.27 241 8 1 1 20 259 63 303 2e-96 343 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA 52GA36-G19CA11AGCA9CA9GA14GA34TG48
V SRR765688.49935 IGHV4-55*09 70.35 226 55 5 12 40 260 77 295 1e-27 114 GTGTCTCTAGCAACAGTGCTGCTTGG---AACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTC--TCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAA GTGACTCCATCAGCAGTGGTAACTGGTGAATCTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGG-GGAAATCCATCAT-----AGTGGGAGCAC-CTACTACAACCCGTCCCTCAAGAGTCGAATCACCATGTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGTGGACACGGCCGTGTATTACTGTGCGAGAA 3TA3TC1GT2AG5CG1GACATC3-T-G-A1AT7AC1GC3TC5TGCG2GAAG2CG2TG6CA1GT1AGA-3CA2AC1TAAT2GTG-T-C-C-C-5TG1TGAC1TCA-2TAAC1GA2GATC1-C-G2TCGC1GC2AGGA7AC5CGGTAC1CGCT6AG21CA1TG4AG9TCCG3AT9TC14AG4
D SRR765688.49935 IGHD7-27*01 100.00 11 0 0 0 270 280 1 11 0.046 22.4 CTAACTGGGGA CTAACTGGGGA 11
D SRR765688.49935 IGHD1-20*01 100.00 7 0 0 0 271 277 5 11 12 14.4 TAACTGG TAACTGG 7
D SRR765688.49935 IGHD1-7*01 100.00 7 0 0 0 271 277 5 11 12 14.4 TAACTGG TAACTGG 7
J SRR765688.49935 IGHJ4*02 95.45 44 2 0 0 289 332 5 48 5e-17 71.9 CTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 7CT15AC20
J SRR765688.49935 IGHJ5*02 95.00 40 2 0 0 293 332 12 51 1e-14 63.9 GACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG GACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4AC14AC20
J SRR765688.49935 IGHJ4*01 93.18 44 3 0 0 289 332 5 48 1e-14 63.9 CTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 7CT10GA4AC20
# IGBLASTN 2.2.29+
# Query: SRR765688.46955 HQP9GKT01BB4XL length=379|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TTCGA CCCTCCAACCGC GTATAGCAGCA CCTGAGTT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 23 62 40 39 1 0 97.5
CDR1-IMGT 63 86 24 20 4 0 83.3
FR2-IMGT 87 137 51 50 1 0 98
CDR2-IMGT 138 161 24 21 3 0 87.5
FR3-IMGT 162 274 114 103 10 1 90.4
CDR3-IMGT (germline) 275 278 4 3 1 0 75
Total N/A N/A 257 236 20 1 91.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46955 IGHV7-4-1*02 91.83 257 20 1 1 23 278 36 292 9e-93 330 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC3-T38GA8AG14AG1AG9TG3
V SRR765688.46955 IGHV7-4-1*01 91.44 257 21 1 1 23 278 36 292 8e-92 327 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC3-T35AT2GA8AG14AG1AG9TG3
V SRR765688.46955 IGHV7-4-1*04 91.44 257 21 1 1 23 278 36 292 8e-92 327 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGA GAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC3-T18CT19GA8AG14AG1AG9TG3
D SRR765688.46955 IGHD6-13*01 100.00 11 0 0 0 291 301 3 13 0.045 22.4 GTATAGCAGCA GTATAGCAGCA 11
D SRR765688.46955 IGHD6-25*01 100.00 10 0 0 0 291 300 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
D SRR765688.46955 IGHD6-6*01 100.00 10 0 0 0 291 300 3 12 0.18 20.4 GTATAGCAGC GTATAGCAGC 10
J SRR765688.46955 IGHJ4*02 100.00 35 0 0 0 310 344 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.46955 IGHJ5*02 100.00 35 0 0 0 310 344 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.46955 IGHJ1*01 97.14 35 1 0 0 310 344 18 52 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.41568 HQP9GKT01AV8YG length=399|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*01,IGHV1-69*04,IGHV1-69*09 IGHD1-26*01,IGHD2-2*02,IGHD2-21*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG GTCGTCTTGTCCTG GTGGGAG TTGCTATATGTCCGCCTC CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 38 1 0 97.4
CDR1-IMGT 60 83 24 17 7 0 70.8
FR2-IMGT 84 134 51 45 6 0 88.2
CDR2-IMGT 135 161 27 17 7 3 63
FR3-IMGT 162 275 114 96 18 0 84.2
CDR3-IMGT (germline) 276 280 5 5 0 0 100
Total N/A N/A 260 218 39 3 83.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.41568 IGHV1-69*01 83.85 260 39 1 3 21 280 37 293 2e-74 269 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCC---CTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAG 30CA9CG6TC5TCTAAG1CT3TC3TCCAAG4CG4GA33CG2TA6C-A-A-1AT1CTTCGT5TATC4CA3TA9CG15GA1CG3TA10GA2TCGA3CG3CA6CA4AGTC19AG9AG1CG2CT11
V SRR765688.41568 IGHV1-69*04 83.85 260 39 1 3 21 280 37 293 2e-74 269 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCC---CTATCCTTGGTATAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAG 30CA9CG6TC5TCTAAG1CT3TC3TCCAAG4CG4GA33CA2TA6C-A-A-1AT1CTTCGC5TA5CA3TA9CG15GA1CG3TA8GA1GA2TCGA3CG3CA6CA4AGTC19AG9AG1CG2CT11
V SRR765688.41568 IGHV1-69*09 83.85 260 39 1 3 21 280 37 293 2e-74 269 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAG AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCC---CTATCCTTGGTATAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACAAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAG 30CA9CG6TC5TCTAAG1CT3TC3TCCAAG4CG4GA33CA2TA6C-A-A-1AT1CTTCGC5TA5CA3TA9CG15GA1CG3TA8GA1GA2TCGA3CG3CA6CA4AGTC19AG9AG1CG2CT11
D SRR765688.41568 IGHD1-26*01 100.00 7 0 0 0 295 301 7 13 12 14.4 GTGGGAG GTGGGAG 7
D SRR765688.41568 IGHD2-2*02 100.00 7 0 0 0 303 309 23 29 12 14.4 TGCTATA TGCTATA 7
D SRR765688.41568 IGHD2-21*01 100.00 7 0 0 0 302 308 19 25 12 14.4 TTGCTAT TTGCTAT 7
J SRR765688.41568 IGHJ4*02 97.67 43 1 0 0 320 362 5 47 8e-19 77.8 CTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 7GT35
J SRR765688.41568 IGHJ4*01 95.35 43 2 0 0 320 362 5 47 2e-16 69.9 CTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCA 7GT10GA24
J SRR765688.41568 IGHJ5*02 100.00 34 0 0 0 329 362 17 50 8e-16 67.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 34
# IGBLASTN 2.2.29+
# Query: SRR765688.24124 HQP9GKT01CMXTP length=364|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR1|DUPCOUNT=8
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01,IGHV4-59*02,IGHV4-59*07 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG G GGATATT CCTATGATCTCTATGGCAATAAAGGAGGT TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 56 24 12 12 0 50
FR2-IMGT 57 108 52 42 9 1 80.8
CDR2-IMGT 109 129 21 11 10 0 52.4
FR3-IMGT 130 243 114 98 16 0 86
CDR3-IMGT (germline) 244 248 5 5 0 0 100
Total N/A N/A 229 180 48 1 78.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.24124 IGHV4-59*01 78.60 229 48 1 1 20 248 63 290 2e-53 199 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAG-CCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12T-12AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
V SRR765688.24124 IGHV4-59*02 78.60 229 48 1 1 20 248 63 290 2e-53 199 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAG-CCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TGCT1GA1CTCAAGCT2TC2TC4CGTC12T-12AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
V SRR765688.24124 IGHV4-59*07 78.60 229 48 1 1 20 248 63 290 2e-53 199 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAG CTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAG-CCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12T-12AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14
D SRR765688.24124 IGHD2-15*01 100.00 7 0 0 0 250 256 2 8 11 14.4 GGATATT GGATATT 7
D SRR765688.24124 IGHD2-2*01 100.00 7 0 0 0 250 256 2 8 11 14.4 GGATATT GGATATT 7
D SRR765688.24124 IGHD2-2*02 100.00 7 0 0 0 250 256 2 8 11 14.4 GGATATT GGATATT 7
J SRR765688.24124 IGHJ4*02 93.02 43 3 0 0 286 328 6 48 4e-14 61.9 TTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 8TC13TC2GT17
J SRR765688.24124 IGHJ4*01 90.70 43 4 0 0 286 328 6 48 1e-11 54.0 TTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 8TC8GA4TC2GT17
J SRR765688.24124 IGHJ5*02 94.12 34 2 0 0 295 328 18 51 4e-11 52.0 TGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 13TC2GT17
# IGBLASTN 2.2.29+
# Query: SRR765688.32089 HQP9GKT01AO7NN length=385|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG GAATATAAGGCGCTCCGATAATACTGC TTATTAT GCCGAGTAT TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 19 5 0 79.2
FR2-IMGT 80 130 51 45 6 0 88.2
CDR2-IMGT 131 154 24 22 2 0 91.7
FR3-IMGT 155 268 114 98 16 0 86
CDR3-IMGT (germline) 269 273 5 4 1 0 80
Total N/A N/A 253 221 32 0 87.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.32089 IGHV5-51*01 87.35 253 32 0 0 21 273 41 293 2e-82 296 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG CCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.32089 IGHV5-51*03 87.25 251 32 0 0 23 273 43 293 2e-81 293 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.32089 IGHV5-51*04 86.85 251 33 0 0 23 273 43 293 1e-80 290 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGCCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA1TC2GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
D SRR765688.32089 IGHD3-10*01 100.00 7 0 0 0 301 307 22 28 12 14.4 TTATTAT TTATTAT 7
D SRR765688.32089 IGHD3-10*02 100.00 7 0 0 0 301 307 21 27 12 14.4 TTATTAT TTATTAT 7
D SRR765688.32089 IGHD3-16*01 100.00 7 0 0 0 298 304 28 34 12 14.4 TGCTTAT TGCTTAT 7
J SRR765688.32089 IGHJ4*02 97.06 34 1 0 0 317 350 15 48 2e-13 60.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 16AT17
J SRR765688.32089 IGHJ5*02 97.06 34 1 0 0 317 350 18 51 2e-13 60.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 16AT17
J SRR765688.32089 IGHJ1*01 94.12 34 2 0 0 317 350 19 52 4e-11 52.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG TGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 11AC4AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.13860 HQP9GKT01AT8BJ length=362|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CAAGA ACTAGATA TAGTAGTAC GCCT TACTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 62 30 26 4 0 86.7
FR2-IMGT 63 112 51 47 3 1 92.2
CDR2-IMGT 113 139 27 25 2 0 92.6
FR3-IMGT 140 253 114 102 12 0 89.5
CDR3-IMGT (germline) 254 259 6 6 0 0 100
Total N/A N/A 241 218 22 1 90.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.13860 IGHV6-1*01 90.46 241 22 1 1 20 259 63 303 2e-83 299 CTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGG-AAGGACCTTCTACAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA 5TC19GT1CTGA9TC13CA13CA18TC3-G6CA1TA24AT2CT3CG1GA3TG11GA1CA9GC31AG30TG1TG5TC9
V SRR765688.13860 IGHV6-1*02 90.46 241 22 1 1 20 259 63 303 2e-83 299 CTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGG-AAGGACCTTCTACAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGA 5TC19GT1CTGA9TC13CA13CA18TC3-G6CA1TA24AT2CT3CG1GA3TG11GA1CA9GC31AG30TG1TG5TC9
V SRR765688.13860 IGHV4-30-2*06 71.72 198 49 3 7 63 259 106 297 2e-28 117 TGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGGAAGGACCTTCTACAGGTCCAAGTGGTATAATGAA-TACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGA TGGAGCTGGATCCGGCAGTCACCAGGGAAGGGCCTGGAGTGGATTG--GGTACATCTA----TCATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAGGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCCGTGTATTACTGTGCCAGA 4AG4CA2AC7CA2CATGCG2GAAG5TG6TA1GT1A-A-2ATCA1TA4C-A-G-G-2CAAT5TG1TGAC1TCGCAT1-C3GACAAC1TC3TCTC1GC2AG14CAATACCA1CT5CGAG21CA1AG4AG9TCCG3AC9TCTG1TG5TC5AC3
D SRR765688.13860 IGHD2-2*01 100.00 9 0 0 0 268 276 10 18 0.67 18.4 TAGTAGTAC TAGTAGTAC 9
D SRR765688.13860 IGHD2-2*02 100.00 9 0 0 0 268 276 10 18 0.67 18.4 TAGTAGTAC TAGTAGTAC 9
D SRR765688.13860 IGHD2-2*03 100.00 9 0 0 0 268 276 10 18 0.67 18.4 TAGTAGTAC TAGTAGTAC 9
J SRR765688.13860 IGHJ4*02 95.65 46 2 0 0 281 326 3 48 3e-18 75.8 TACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG TACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17TC6TA21
J SRR765688.13860 IGHJ4*01 93.48 46 3 0 0 281 326 3 48 7e-16 67.9 TACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG TACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 17TC2GA3TA21
J SRR765688.13860 IGHJ4*03 91.30 46 4 0 0 281 326 3 48 2e-13 60.0 TACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG TACTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 17TC2GA2AGTA21
# IGBLASTN 2.2.29+
# Query: SRR765688.20740 HQP9GKT01ANHJ2 length=378|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR9|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-58*01 IGHD4-23*01 IGHJ6*02,IGHJ6*04 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCAGA AGG CTACGGTGGTAA TCCCCTACAGTATTATAATAATGAA GGTAT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 59 39 36 3 0 92.3
CDR1-IMGT 60 83 24 22 2 0 91.7
FR2-IMGT 84 134 51 46 5 0 90.2
CDR2-IMGT 135 158 24 22 2 0 91.7
FR3-IMGT 159 272 114 105 9 0 92.1
CDR3-IMGT (germline) 273 280 8 8 0 0 100
Total N/A N/A 260 239 21 0 91.9
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.20740 IGHV1-58*01 91.92 260 21 0 0 21 280 37 296 5e-96 341 AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGA AAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATTCACCTTTACTAGCTCTGCTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCACCATTACCAGGGACATGTCCACAAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTACTGTGCGGCAGA 26TC2TCCA15GC9TC20CA3AG4CT5GA17TA11CT9CA36GC5TCTC16TGTCTA3TC4CA43TC11
V SRR765688.20740 IGHV1-58*02 91.54 260 22 0 0 21 280 37 296 4e-95 338 AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGA AAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATTCACCTTTACTAGCTCTGCTATGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTGAGTGGATAGGATGGATCGTCGTTGGCAGTGGTAACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCACCATTACCAGGGACATGTCCACAAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTACTGTGCGGCAGA 26TC2TCCA15GC9TC6GA13CA3AG4CT5GA17TA11CT9CA36GC5TCTC16TGTCTA3TC4CA43TC11
V SRR765688.20740 IGHV1-3*01 81.15 260 49 0 0 21 280 37 296 8e-70 254 AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGA AAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGCATTGGGTGCGCCAGGCCCCCGGACAAAGGCTTGAGTGGATGGGATGGATCAACGCTGGCAATGGTAACACAAAATATTCACAGAAGTTCCAGGGCAGAGTCACCATTACCAGGGACACATCCGCGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGAGA 9AG13CT2TC2TCCA12TA2GC3TC5TC1CA4GA4GT12AG1TC1GC6GACA1CG11AG2TA6GATA2TCCT4GA4CA7CA2CTGT15AGAC7GC5TCTC7TCGA3AG1AG1TGTCTA3TC4CA22CT2GA8CT8TC6GACG3
D SRR765688.20740 IGHD4-23*01 100.00 12 0 0 0 284 295 4 15 0.011 24.4 CTACGGTGGTAA CTACGGTGGTAA 12
D SRR765688.20740 IGHD4-11*01 100.00 8 0 0 0 300 307 4 11 2.7 16.4 CTACAGTA CTACAGTA 8
D SRR765688.20740 IGHD4-17*01 100.00 8 0 0 0 284 291 4 11 2.7 16.4 CTACGGTG CTACGGTG 8
J SRR765688.20740 IGHJ6*02 100.00 18 0 0 0 321 338 18 35 2e-06 36.2 GGTATGGACGTCTGGGGC GGTATGGACGTCTGGGGC 18
J SRR765688.20740 IGHJ6*04 100.00 18 0 0 0 321 338 18 35 2e-06 36.2 GGTATGGACGTCTGGGGC GGTATGGACGTCTGGGGC 18
J SRR765688.20740 IGHJ6*01 100.00 17 0 0 0 321 337 18 34 1e-05 34.2 GGTATGGACGTCTGGGG GGTATGGACGTCTGGGG 17
# IGBLASTN 2.2.29+
# Query: SRR765688.15114 HQP9GKT01DWQ8O length=372|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR8|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV2-70*13 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ6*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCACG TGTTTACGGGG CTGGAA T CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 23 39 17 16 1 0 94.1
CDR1-IMGT 40 69 30 26 4 0 86.7
FR2-IMGT 70 121 52 48 3 1 92.3
CDR2-IMGT 122 142 21 21 0 0 100
FR3-IMGT 143 254 114 108 4 2 94.7
CDR3-IMGT (germline) 255 259 5 5 0 0 100
Total N/A N/A 239 224 12 3 93.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.15114 IGHV2-70*13 93.72 239 12 2 3 23 259 59 296 4e-88 315 TGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACG TGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGTGTGTGAGCTGGATCCGTCA-GCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACTCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTATTGTGCACG 8TA21CG1GA1CT1CG14CG12C-18TG11GA26TC4AG40AT1-C-A52AG14
V SRR765688.15114 IGHV2-70*12 93.70 238 12 2 3 23 258 59 295 1e-87 313 TGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCAC TGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGTGTGTGAGCTGGATCCGTCA-GCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACTCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTACTGTGCAC 8TA21CG1GA1CT1CG14CG12C-18TG11GA26TC4AG40AT1-C-A58TC7
V SRR765688.15114 IGHV2-70*01 93.31 239 13 2 3 23 259 59 296 4e-87 311 TGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACG TGACCTGCACCTTCTCTGGGTTCTCACTCAGCACTAGTGGAATGTGTGTGAGCTGGATCCGTCA-GCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACTCATTGATTGGGATGATGATAAATACTACAGCACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCTCCAAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACGTATTACTGTGCACG 8TA21CG1GA1CT1CG14CG12C-18TG11GA26TC4AG40AT1-C-A52AG5TC8
D SRR765688.15114 IGHD1-1*01 100.00 6 0 0 0 271 276 8 13 44 12.4 CTGGAA CTGGAA 6
D SRR765688.15114 IGHD1-20*01 100.00 6 0 0 0 271 276 8 13 44 12.4 CTGGAA CTGGAA 6
D SRR765688.15114 IGHD1-7*01 100.00 6 0 0 0 271 276 8 13 44 12.4 CTGGAA CTGGAA 6
J SRR765688.15114 IGHJ6*02 96.55 58 2 0 0 278 335 5 62 2e-25 99.6 CTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA CTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 11TA2CG43
J SRR765688.15114 IGHJ6*01 94.92 59 3 0 0 278 336 5 63 1e-23 93.7 CTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG CTACTACTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 11TA2CG15CG28
J SRR765688.15114 IGHJ6*04 94.92 59 3 0 0 278 336 5 63 1e-23 93.7 CTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG CTACTACTACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 11TA2CG16CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.44373 HQP9GKT01A960E length=371|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01,IGHV4-59*07 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG CCTCAAGA GGGGAG GAGACTT CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 20 4 0 83.3
FR2-IMGT 71 121 51 49 2 0 96.1
CDR2-IMGT 122 142 21 17 4 0 81
FR3-IMGT 143 256 114 105 9 0 92.1
CDR3-IMGT (germline) 257 261 5 5 0 0 100
Total N/A N/A 241 222 19 0 92.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.44373 IGHV4-59*01 92.12 241 19 0 0 21 261 50 290 5e-89 318 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 33TC3GC2GT3GT10TC42CT10TC1AG4GATG11GA18GA3GA9CT31TC2CA4CT1CT21AG14
V SRR765688.44373 IGHV4-59*07 92.12 241 19 0 0 21 261 50 290 5e-89 318 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 33TC3GC2GT3GT10TC42CT10TC1AG4GATG11GA18GA3GA9CT31TC2CA4CT1CT21AG14
V SRR765688.44373 IGHV4-59*02 91.70 241 20 0 0 21 261 50 290 4e-88 315 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAG 33TC1AG1GC2GT3GT10TC42CT10TC1AG4GATG11GA18GA3GA9CT31TC2CA4CT1CT21AG14
D SRR765688.44373 IGHD3-10*01 100.00 6 0 0 0 270 275 16 21 44 12.4 GGGGAG GGGGAG 6
D SRR765688.44373 IGHD3-10*02 100.00 6 0 0 0 270 275 15 20 44 12.4 GGGGAG GGGGAG 6
D SRR765688.44373 IGHD3-16*01 100.00 6 0 0 0 270 275 19 24 44 12.4 GGGGAG GGGGAG 6
J SRR765688.44373 IGHJ6*02 96.15 52 2 0 0 283 334 11 62 8e-22 87.7 CTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 8CG1TA41
J SRR765688.44373 IGHJ6*01 94.34 53 3 0 0 283 335 11 63 5e-20 81.8 CTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG CTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 8CG1TA13CG28
J SRR765688.44373 IGHJ6*04 94.34 53 3 0 0 283 335 11 63 5e-20 81.8 CTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG CTACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 8CG1TA14CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.22020 HQP9GKT01BU60O length=404|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-1*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GGGATATTATATTGA TACAACTGG TTCTCCGAGGTCAC ACGGT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 41 1 0 97.6
CDR1-IMGT 63 86 24 18 6 0 75
FR2-IMGT 87 137 51 48 3 0 94.1
CDR2-IMGT 138 161 24 21 3 0 87.5
FR3-IMGT 162 275 114 104 10 0 91.2
CDR3-IMGT (germline) 276 283 8 8 0 0 100
Total N/A N/A 263 240 23 0 91.3
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.22020 IGHV7-4-1*02 91.25 263 23 0 0 21 283 34 296 2e-95 339 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 26CT16CG5TCTC5AT1AGGC6CA3CA24GA30GAGA7GA18CG3TC8TG11TC1CT30CG6GA1TC23TG5TC11
V SRR765688.22020 IGHV7-4-1*04 90.87 263 24 0 0 21 283 34 296 1e-94 336 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 26CT16CG5TCTC5AT1AGGC6CA3CA24GA30GAGA7GA18CG3TC8TG11TC1CT17CT12CG6GA1TC23TG5TC11
V SRR765688.22020 IGHV7-4-1*01 90.80 261 24 0 0 21 281 34 294 1e-93 333 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 26CT16CG5TCTC5AT1AGGC6CA3CA24GA30GAGA7GA18CG3TC8TG11TC1CT30CG3AT2GA1TC23TG5TC9
D SRR765688.22020 IGHD1-1*01 100.00 9 0 0 0 299 307 3 11 0.76 18.4 TACAACTGG TACAACTGG 9
D SRR765688.22020 IGHD2-15*01 100.00 7 0 0 0 285 291 2 8 12 14.4 GGATATT GGATATT 7
D SRR765688.22020 IGHD2-2*01 100.00 7 0 0 0 285 291 2 8 12 14.4 GGATATT GGATATT 7
J SRR765688.22020 IGHJ6*02 95.74 47 2 0 0 322 368 16 62 8e-19 77.8 ACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA ACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 5TA23GA17
J SRR765688.22020 IGHJ6*01 93.75 48 3 0 0 322 369 16 63 5e-17 71.9 ACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAG ACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 5TA13CG9GA18
J SRR765688.22020 IGHJ6*04 93.75 48 3 0 0 322 369 16 63 5e-17 71.9 ACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAG ACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 5TA14CA8GA18
# IGBLASTN 2.2.29+
# Query: SRR765688.23018 HQP9GKT01DGIK6 length=395|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GATATT CCTACGACCTCGATGGCAATTATGGTGG CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 24 2 0 92.3
CDR1-IMGT 47 70 24 16 8 0 66.7
FR2-IMGT 71 121 51 44 7 0 86.3
CDR2-IMGT 122 154 33 13 8 12 39.4
FR3-IMGT 155 268 114 99 15 0 86.8
CDR3-IMGT (germline) 269 275 7 7 0 0 100
Total N/A N/A 255 203 40 12 79.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.23018 IGHV4-59*02 79.61 255 40 1 12 21 275 50 292 1e-62 230 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCA------------CCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3G-C-A-A-G-T-C-C-G-A-C-C-4GC18GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.23018 IGHV4-59*01 79.22 255 41 1 12 21 275 50 292 8e-62 227 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCA------------CCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3G-C-A-A-G-T-C-C-G-A-C-C-4GC18GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.23018 IGHV4-59*07 79.13 254 41 1 12 21 274 50 291 2e-61 226 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCA------------CCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3G-C-A-A-G-T-C-C-G-A-C-C-4GC18GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA10
D SRR765688.23018 IGHD2-15*01 100.00 8 0 0 0 274 281 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.23018 IGHD2-2*01 100.00 8 0 0 0 274 281 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.23018 IGHD2-2*02 100.00 8 0 0 0 274 281 1 8 2.9 16.4 AGGATATT AGGATATT 8
J SRR765688.23018 IGHJ4*02 93.18 44 3 0 0 310 353 5 48 1e-14 63.9 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23TC2GT10GT6
J SRR765688.23018 IGHJ4*01 90.91 44 4 0 0 310 353 5 48 3e-12 56.0 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 18GA4TC2GT10GT6
J SRR765688.23018 IGHJ4*03 88.64 44 5 0 0 310 353 5 48 7e-10 48.1 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 18GA2AG1TC2GT10GT6
# IGBLASTN 2.2.29+
# Query: SRR765688.46548 HQP9GKT01BMT60 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGAGA GAGTCTCGGCC ACTGGA N/A TTGAC
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 37 5 0 88.1
CDR1-IMGT 63 86 24 22 2 0 91.7
FR2-IMGT 87 137 51 51 0 0 100
CDR2-IMGT 138 161 24 24 0 0 100
FR3-IMGT 162 275 114 102 12 0 89.5
CDR3-IMGT (germline) 276 283 8 7 1 0 87.5
Total N/A N/A 263 243 20 0 92.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46548 IGHV7-4-1*02 92.40 263 20 0 0 21 283 34 296 2e-98 349 AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG5TG5CG11AG1CT9TA9AG84AG10CG25CT16TC3AG8GC6AG2AG12AG4GC4AG1AG10GC6
V SRR765688.46548 IGHV7-4-1*04 92.02 263 21 0 0 21 283 34 296 2e-97 346 AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGA 12AG5TG5CG11AG1CT9TA9AG84AG10CG25CT16TC1CT1AG8GC6AG2AG12AG4GC4AG1AG10GC6
V SRR765688.46548 IGHV7-4-1*01 91.95 261 21 0 0 21 281 34 294 2e-96 343 AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 12AG5TG5CG11AG1CT9TA9AG84AG10CG25CT16TC3AG8GC5ATAG2AG12AG4GC4AG1AG10GC4
D SRR765688.46548 IGHD1-1*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.46548 IGHD1-20*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
D SRR765688.46548 IGHD1-7*01 100.00 6 0 0 0 295 300 7 12 45 12.4 ACTGGA ACTGGA 6
J SRR765688.46548 IGHJ4*02 100.00 42 0 0 0 301 342 7 48 1e-20 83.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 42
J SRR765688.46548 IGHJ4*01 97.62 42 1 0 0 301 342 7 48 3e-18 75.8 TTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 16GA25
J SRR765688.46548 IGHJ5*02 100.00 35 0 0 0 308 342 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
# IGBLASTN 2.2.29+
# Query: SRR765688.11345 HQP9GKT01A2AIW length=375|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGACA TTCCGGGCCTAAC AGTGTCT TGTT CTGGT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 20 4 0 83.3
FR2-IMGT 71 121 51 48 3 0 94.1
CDR2-IMGT 122 142 21 17 4 0 81
FR3-IMGT 143 255 114 101 12 1 88.6
CDR3-IMGT (germline) 256 263 8 8 0 0 100
Total N/A N/A 244 220 23 1 90.2
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.11345 IGHV4-59*08 90.16 244 23 1 1 21 263 50 293 7e-84 300 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14TC5TC7GA5AGTC3CG25CT1TA12
V SRR765688.11345 IGHV4-4*08 89.26 242 25 1 1 21 261 50 291 5e-81 291 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GAAC2TG2AG2CG4TA1TC5-C29AC1CTGA14TC5TC7GA5AGTC3CG25CT1TA10
V SRR765688.11345 IGHV4-59*01 89.26 242 25 1 1 21 261 50 291 5e-81 291 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14TC5TC7GA5AGTC3CG7CT2AG14CT1TA10
D SRR765688.11345 IGHD5/OR15-5a*01 100.00 7 0 0 0 277 283 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.11345 IGHD5/OR15-5b*01 100.00 7 0 0 0 277 283 9 15 11 14.4 AGTGTCT AGTGTCT 7
D SRR765688.11345 IGHD2-21*01 100.00 5 0 0 0 263 267 24 28 175 10.4 ATTCC ATTCC 5
J SRR765688.11345 IGHJ5*02 97.87 47 0 1 1 288 333 5 51 7e-19 77.8 CTGGTTCGACCC-TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 12-C34
J SRR765688.11345 IGHJ4*02 100.00 35 0 0 0 299 333 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.11345 IGHJ5*01 95.74 47 1 1 1 288 333 5 51 2e-16 69.9 CTGGTTCGAC-CCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGTTCGACTCCTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 10-T10GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.43574 HQP9GKT01D10PE length=310|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgD-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 N/A N/A VH No N/A N/A +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CCGTG N/A N/A N/A N/A
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 42 0 0 100
CDR1-IMGT 63 86 24 24 0 0 100
FR2-IMGT 87 137 51 51 0 0 100
CDR2-IMGT 138 161 24 24 0 0 100
FR3-IMGT 162 266 105 105 0 0 100
Total N/A N/A 246 246 0 0 100
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 3 hits found
V SRR765688.43574 IGHV7-4-1*02 100.00 246 0 0 0 21 266 34 279 3e-109 385 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG 246
V SRR765688.43574 IGHV7-4-1*01 99.59 246 1 0 0 21 266 34 279 2e-108 382 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTG 216AT29
V SRR765688.43574 IGHV7-4-1*04 99.59 246 1 0 0 21 266 34 279 2e-108 382 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTG 199CT46
# IGBLASTN 2.2.29+
# Query: SRR765688.36594 HQP9GKT01B0W9F length=368|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*01,IGHV7-4-1*02 IGHD6-19*01,IGHD3-10*01,IGHD3-10*02 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
TGCGA C GCAGT CGGGATCACGCCT CTGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 42 0 0 100
CDR1-IMGT 63 86 24 21 3 0 87.5
FR2-IMGT 87 137 51 47 4 0 92.2
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 94 20 0 82.5
CDR3-IMGT (germline) 276 279 4 4 0 0 100
Total N/A N/A 259 228 31 0 88
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.36594 IGHV7-4-1*01 88.03 259 31 0 0 21 279 34 292 3e-86 308 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 58CGTC1CA6AG14CG14CG3AG23GACA9TCTC3ACTG1TACT7AG12CG12GT5TA20TC2GC1AG3CTTG2CGTC2CA3CG4AG9AG1TG13
V SRR765688.36594 IGHV7-4-1*02 88.03 259 31 0 0 21 279 34 292 3e-86 308 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 58CGTC1CA6AG14CG14CG3AG23GACA9TCTC3ACTG1TACT7AG12CG12GT5TA20TC2GC1AG3CATG2CGTC2CA3CG4AG9AG1TG13
V SRR765688.36594 IGHV7-4-1*04 87.64 259 32 0 0 21 279 34 292 3e-85 305 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGA 58CGTC1CA6AG14CG14CG3AG23GACA9TCTC3ACTG1TACT7AG12CG12GT5TA12CT7TC2GC1AG3CATG2CGTC2CA3CG4AG9AG1TG13
D SRR765688.36594 IGHD6-19*01 100.00 5 0 0 0 281 285 8 12 175 10.4 GCAGT GCAGT 5
D SRR765688.36594 IGHD3-10*01 100.00 5 0 0 0 285 289 14 18 175 10.4 TCGGG TCGGG 5
D SRR765688.36594 IGHD3-10*02 100.00 5 0 0 0 285 289 13 17 175 10.4 TCGGG TCGGG 5
J SRR765688.36594 IGHJ4*02 100.00 35 0 0 0 299 333 14 48 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.36594 IGHJ5*02 100.00 35 0 0 0 299 333 17 51 2e-16 69.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 35
J SRR765688.36594 IGHJ1*01 97.14 35 1 0 0 299 333 18 52 4e-14 61.9 CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 12AC22
# IGBLASTN 2.2.29+
# Query: SRR765688.47496 HQP9GKT01DBCHA length=377|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-34*01,IGHV4-34*02 IGHD3-9*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG GGCCCGTGGTGA TGGTTATTATAA TGATCGATTACGA TTTGA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 26 0 0 100
CDR1-IMGT 47 70 24 21 3 0 87.5
FR2-IMGT 71 121 51 45 6 0 88.2
CDR2-IMGT 122 142 21 20 1 0 95.2
FR3-IMGT 143 256 114 99 15 0 86.8
CDR3-IMGT (germline) 257 261 5 5 0 0 100
Total N/A N/A 241 216 25 0 89.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.47496 IGHV4-34*01 89.63 241 25 0 0 21 261 50 290 2e-83 299 CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 45TA1GTCA5CG9TC3TC9TA18AG2TACA43GT7GC1TA1TAAT8TA7AG1GATC2CG10GA5CGTC26CG8CT5
V SRR765688.47496 IGHV4-34*02 89.63 241 25 0 0 21 261 50 290 2e-83 299 CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 45TA1GTCA5CG9TC3TC9TA18AG2TACA43GT7GC1TA1TAAT8TA7AG1GATC2CG10GA5CGTC26CG8CT5
V SRR765688.47496 IGHV4-34*12 89.21 241 26 0 0 21 261 50 290 2e-82 296 CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCATTCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 45TA1GTCA5CG9TC3TC9TA18AG2TACA3AT39GT7GC1TA1TAAT8TA7AG1GATC2CG10GA5CGTC26CG8CT5
D SRR765688.47496 IGHD3-9*01 100.00 12 0 0 0 274 285 19 30 0.011 24.4 TGGTTATTATAA TGGTTATTATAA 12
D SRR765688.47496 IGHD3-3*01 100.00 11 0 0 0 274 284 19 29 0.045 22.4 TGGTTATTATA TGGTTATTATA 11
D SRR765688.47496 IGHD3-3*02 100.00 11 0 0 0 274 284 19 29 0.045 22.4 TGGTTATTATA TGGTTATTATA 11
J SRR765688.47496 IGHJ4*02 95.35 43 2 0 0 299 341 6 48 2e-16 69.9 TTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 7TAGC34
J SRR765688.47496 IGHJ5*02 100.00 34 0 0 0 308 341 18 51 7e-16 67.9 TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 34
J SRR765688.47496 IGHJ4*01 93.02 43 3 0 0 299 341 6 48 4e-14 61.9 TTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG TTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 7TAGC8GA25
# IGBLASTN 2.2.29+
# Query: SRR765688.46707 HQP9GKT01BT4GG length=359|SEQORIENT=F,F,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR1|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV6-1*01,IGHV6-1*02 IGHD5-12*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GGAGA CAGGTATA ATAGTGGC GGCGGA GACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 32 13 12 1 0 92.3
CDR1-IMGT 33 62 30 24 6 0 80
FR2-IMGT 63 113 51 48 3 0 94.1
CDR2-IMGT 114 140 27 22 5 0 81.5
FR3-IMGT 141 254 114 93 21 0 81.6
CDR3-IMGT (germline) 255 262 8 7 1 0 87.5
Total N/A N/A 243 206 37 0 84.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.46707 IGHV6-1*01 84.77 243 37 0 0 20 262 63 305 4e-73 265 CTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 5TC9CG14TCGA4ATAGTC7CA5GA7AG36ATTAGCCT8GA19CATG5AG4GA8TA1TCTC2TC1GAGC8GA4GA7CT3TCTC5AGTC3CA25AG1TG5TC6GA4
V SRR765688.46707 IGHV6-1*02 84.77 243 37 0 0 20 262 63 305 4e-73 265 CTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGA CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAGA 5TC9CG14TCGA4ATAGTC7CA5GA7AG36ATTAGCCT8GA19CATG5AG4GA8TA1TCTC2TC1GAGC8GA4GA7CT3TCTC5AGTC3CA25AG1TG5TC6GA4
V SRR765688.46707 IGHV4-30-2*05 75.00 104 26 0 0 159 262 196 299 3e-18 82.9 AAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGA AAGAGTCGAGTTACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCGCAGACACGGCCGTGTATTACTGTGCCAGAGA 9AG3TCTC2TAATGCGACGCT11GA7CT3TCTC3CA1AGTC3CG10CG3ACGA8TCAG1TG5TC5ACGA4
D SRR765688.46707 IGHD5-12*01 100.00 8 0 0 0 271 278 7 14 2.7 16.4 ATAGTGGC ATAGTGGC 8
D SRR765688.46707 IGHD1-14*01 100.00 7 0 0 0 265 271 1 7 11 14.4 GGTATAA GGTATAA 7
D SRR765688.46707 IGHD1-20*01 100.00 7 0 0 0 265 271 1 7 11 14.4 GGTATAA GGTATAA 7
J SRR765688.46707 IGHJ4*02 97.50 40 1 0 0 285 324 9 48 4e-17 71.9 GACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 19TC20
J SRR765688.46707 IGHJ4*01 95.00 40 2 0 0 285 324 9 48 1e-14 63.9 GACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 14GA4TC20
J SRR765688.46707 IGHJ5*02 97.14 35 1 0 0 290 324 17 51 4e-14 61.9 CTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 14TC20
# IGBLASTN 2.2.29+
# Query: SRR765688.35420 HQP9GKT01C0N6S length=397|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-16*02,IGHD3-3*01,IGHD3-3*02 IGHJ6*02,IGHJ6*04 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AGGCA TGGATTATACGGTTGTGATCATACCGGCT GTTATAC AAGCTT CTACT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 18 6 0 75
FR2-IMGT 80 130 51 47 4 0 92.2
CDR2-IMGT 131 154 24 19 5 0 79.2
FR3-IMGT 155 268 114 104 10 0 91.2
CDR3-IMGT (germline) 269 276 8 7 1 0 87.5
Total N/A N/A 256 228 28 0 89.1
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.35420 IGHV5-51*01 89.06 256 28 0 0 21 276 41 296 1e-87 313 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCA CCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACA 15GA15CT11GC2CT1GC1CAAG3TC7CG41CGCG2GC10AG3GC1TC5TCTC5TC4AC9GA19TC24TCAC9AG20GC6AG4TA9GA2
V SRR765688.35420 IGHV5-51*03 89.24 251 27 0 0 23 273 43 293 3e-86 308 GGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 13GA15CT11GC2CT1GC1CAAG3TC7CG41CGCG2GC10AG3GC1TC5TCTC5TC4AC9GA19TC24TCAC9AG20GC6AG4TA9
V SRR765688.35420 IGHV5-51*02 88.28 256 30 0 0 21 276 41 296 1e-85 307 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCA CCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGACCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCTTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACA 15GA15CT11GC2CT1GC1CAAG3TC4TC2CG28CT12CGCG2GC10AG3GC1TC5TCTC5TC4AC9GA19TC24TCAC9AG20GC6AG4TA9GA2
D SRR765688.35420 IGHD3-16*02 100.00 7 0 0 0 306 312 30 36 11 14.4 GTTATAC GTTATAC 7
D SRR765688.35420 IGHD3-3*01 100.00 7 0 0 0 280 286 24 30 11 14.4 ATTATAC ATTATAC 7
D SRR765688.35420 IGHD3-3*02 100.00 7 0 0 0 280 286 24 30 11 14.4 ATTATAC ATTATAC 7
J SRR765688.35420 IGHJ6*02 96.43 28 1 0 0 319 346 8 35 7e-10 48.1 CTACTACTACGGGATGGACGTCTGGGGC CTACTACTACGGTATGGACGTCTGGGGC 12GT15
J SRR765688.35420 IGHJ6*04 96.43 28 1 0 0 319 346 8 35 7e-10 48.1 CTACTACTACGGGATGGACGTCTGGGGC CTACTACTACGGTATGGACGTCTGGGGC 12GT15
J SRR765688.35420 IGHJ6*01 96.30 27 1 0 0 319 345 8 34 3e-09 46.1 CTACTACTACGGGATGGACGTCTGGGG CTACTACTACGGTATGGACGTCTGGGG 12GT14
# IGBLASTN 2.2.29+
# Query: SRR765688.35321 HQP9GKT01C64W5 length=377|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR5|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-10-1*01,IGHV5-10-1*03 IGHD4-17*01 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG CCGCTCGGG TGACTACGGTGACT TCGAGTTTGCCC ACTGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 34 1 0 97.1
CDR1-IMGT 56 79 24 20 4 0 83.3
FR2-IMGT 80 131 52 47 4 1 90.4
CDR2-IMGT 132 155 24 21 3 0 87.5
FR3-IMGT 156 269 114 108 6 0 94.7
CDR3-IMGT (germline) 270 274 5 5 0 0 100
Total N/A N/A 254 235 18 1 92.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.35321 IGHV5-10-1*01 92.52 254 18 1 1 21 274 41 293 3e-93 332 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAG CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGG-AGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 30CG12TC5GC2TC2TC5GC1CG12AGTA29G-6AG4CT9GT7GC37TC14CG20CG2AG30TC8
V SRR765688.35321 IGHV5-10-1*03 92.52 254 18 1 1 21 274 41 293 3e-93 332 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAG CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGG-AGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 30CG12TC5GC2TC2TC5GC1CG12AGTA29G-6AG4CT9GT7GC37TC14CG20CG2AG30TC8
V SRR765688.35321 IGHV5-10-1*04 92.13 254 19 1 1 21 274 41 293 3e-92 329 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAG CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGG-AGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 30CG12TC5GC2TC2TC5GC1CG12AGTA29G-6AG4CT9GT7GC23CG13TC14CG20CG2AG30TC8
D SRR765688.35321 IGHD4-17*01 100.00 14 0 0 0 284 297 1 14 7e-04 28.4 TGACTACGGTGACT TGACTACGGTGACT 14
D SRR765688.35321 IGHD4-23*01 100.00 11 0 0 0 284 294 1 11 0.045 22.4 TGACTACGGTG TGACTACGGTG 11
D SRR765688.35321 IGHD4-17*01 100.00 9 0 0 0 282 290 8 16 0.71 18.4 GGTGACTAC GGTGACTAC 9
J SRR765688.35321 IGHJ4*02 97.22 36 1 0 0 310 345 13 48 1e-14 63.9 ACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG ACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 24TC11
J SRR765688.35321 IGHJ5*02 97.14 35 1 0 0 311 345 17 51 5e-14 61.9 CTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23TC11
J SRR765688.35321 IGHJ1*01 94.59 37 2 0 0 309 345 16 52 7e-13 58.0 CACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG CACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 14AC10TC11
# IGBLASTN 2.2.29+
# Query: SRR765688.40878 HQP9GKT01B4JCW length=380|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR7|DUPCOUNT=3
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*01,IGHV4-59*07 IGHD2-8*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA ATTAGGAC ATATTGTACTA CTGACAGTTGTTCTTTCG ACTTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 25 1 0 96.2
CDR1-IMGT 47 70 24 22 2 0 91.7
FR2-IMGT 71 121 51 47 4 0 92.2
CDR2-IMGT 122 142 21 17 4 0 81
FR3-IMGT 143 256 114 107 7 0 93.9
CDR3-IMGT (germline) 257 262 6 6 0 0 100
Total N/A N/A 242 224 18 0 92.6
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.40878 IGHV4-59*01 92.56 242 18 0 0 21 262 50 291 2e-90 322 CCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 20AG18AG1GA12CG13TC6AG18AT8TCCT4TC7CG15GC14GA14GA28GA1TC29CG1AG15
V SRR765688.40878 IGHV4-59*07 92.56 242 18 0 0 21 262 50 291 2e-90 322 CCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 20AG18AG1GA12CG13TC6AG18AT8TCCT4TC7CG15GC14GA14GA28GA1TC29CG1AG15
V SRR765688.40878 IGHV4-59*02 92.15 242 19 0 0 21 262 50 291 2e-89 319 CCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 20AG14AG3AG1GA12CG13TC6AG18AT8TCCT4TC7CG15GC14GA14GA28GA1TC29CG1AG15
D SRR765688.40878 IGHD2-8*01 100.00 11 0 0 0 271 281 4 14 0.045 22.4 ATATTGTACTA ATATTGTACTA 11
D SRR765688.40878 IGHD2-8*02 100.00 10 0 0 0 271 280 4 13 0.18 20.4 ATATTGTACT ATATTGTACT 10
D SRR765688.40878 IGHD2-15*01 100.00 8 0 0 0 271 278 4 11 2.8 16.4 ATATTGTA ATATTGTA 8
J SRR765688.40878 IGHJ4*02 95.56 45 2 0 0 300 344 4 48 1e-17 73.8 ACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG ACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 10TC21TA12
J SRR765688.40878 IGHJ4*01 93.33 45 3 0 0 300 344 4 48 3e-15 65.9 ACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG ACTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 10TC8GA12TA12
J SRR765688.40878 IGHJ5*02 97.06 34 1 0 0 311 344 18 51 2e-13 60.0 TGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21TA12
# IGBLASTN 2.2.29+
# Query: SRR765688.44256 HQP9GKT01DI3B9 length=395|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR6|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV3-30-3*02 IGHD1-14*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
AAAGA CAG GGAACCA ACTTATATTCACTA TATGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 20 73 54 53 1 0 98.1
CDR1-IMGT 74 97 24 19 5 0 79.2
FR2-IMGT 98 148 51 49 2 0 96.1
CDR2-IMGT 149 172 24 18 6 0 75
FR3-IMGT 173 286 114 107 7 0 93.9
CDR3-IMGT (germline) 287 294 8 8 0 0 100
Total N/A N/A 275 254 21 0 92.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.44256 IGHV3-30-3*02 92.36 275 21 0 0 20 294 22 296 5e-103 364 GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGA GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGCAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAAAGA 50CG19CGTCCT2TGGC1GA1AG50GA3ATTA11CA1GAGA39TC19TC7TC18CG1AG4AG11AG17
V SRR765688.44256 IGHV3-30-3*01 92.62 271 20 0 0 20 290 22 292 4e-102 361 GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGA GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGCAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGA 70CGTCCT2TGGC1GA1AG50GA3ATTA11CA1GAGA39TC19TC7TC18CG1AG4AG11AG13
V SRR765688.44256 IGHV3-30*04 92.00 275 22 0 0 20 294 22 296 4e-102 361 GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGA GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGGACACGGCTGTGTATTACTGTGCGAGAGA 70CGTCCT2TGGC1GA1AG50GA3ATTA9CT1CA1GAGA39TC19TC7TC18CG1AG4AG11AG13AG3
D SRR765688.44256 IGHD1-14*01 100.00 7 0 0 0 298 304 10 16 12 14.4 GGAACCA GGAACCA 7
D SRR765688.44256 IGHD3-16*01 100.00 6 0 0 0 306 311 30 35 48 12.4 CTTATA CTTATA 6
D SRR765688.44256 IGHD1-1*01 100.00 5 0 0 0 298 302 10 14 190 10.4 GGAAC GGAAC 5
J SRR765688.44256 IGHJ6*02 100.00 43 0 0 0 319 361 20 62 3e-21 85.7 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 43
J SRR765688.44256 IGHJ6*01 97.73 44 1 0 0 319 362 20 63 2e-19 79.8 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCCTCAG 15CG28
J SRR765688.44256 IGHJ6*04 97.73 44 1 0 0 319 362 20 63 2e-19 79.8 TATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG TATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG 16CA27
# IGBLASTN 2.2.29+
# Query: SRR765688.42968 HQP9GKT01B3HAW length=382|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgA-PCR|VPRIMER=LR2|DUPCOUNT=5
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV1-69*01,IGHV1-69*12,IGHV1-69D*01 IGHD6-13*01 IGHJ6*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GAGAG GGTCCCA AGCAGCTG AT TACTA
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 35 0 0 100
CDR1-IMGT 56 79 24 21 3 0 87.5
FR2-IMGT 80 130 51 51 0 0 100
CDR2-IMGT 131 154 24 23 1 0 95.8
FR3-IMGT 155 268 114 99 15 0 86.8
CDR3-IMGT (germline) 269 275 7 7 0 0 100
Total N/A N/A 255 236 19 0 92.5
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.42968 IGHV1-69*01 92.55 255 19 0 0 21 275 41 295 1e-95 339 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 43GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.42968 IGHV1-69*12 92.55 255 19 0 0 21 275 41 295 1e-95 339 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 43GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
V SRR765688.42968 IGHV1-69D*01 92.55 255 19 0 0 21 275 41 295 1e-95 339 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAG CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAGCTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGTACAGCAAACTACGCACAGAAGTTCCAGGGCAGAGTCACGATTACCGCGGACGAATCCACGAGCACAGCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAG 43GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16
D SRR765688.42968 IGHD6-13*01 100.00 8 0 0 0 283 290 10 17 2.9 16.4 AGCAGCTG AGCAGCTG 8
D SRR765688.42968 IGHD3-16*01 100.00 7 0 0 0 289 295 7 13 11 14.4 TGATTAC TGATTAC 7
D SRR765688.42968 IGHD3-16*02 100.00 7 0 0 0 289 295 7 13 11 14.4 TGATTAC TGATTAC 7
J SRR765688.42968 IGHJ6*02 98.04 51 1 0 0 293 343 9 59 1e-23 93.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCC 38AG12
J SRR765688.42968 IGHJ6*01 96.08 51 2 0 0 293 343 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGGCAAGGGACCACGGTCACCGTCTCC 26CG11AG12
J SRR765688.42968 IGHJ6*04 96.08 51 2 0 0 293 343 9 59 3e-21 85.7 TACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC TACTACTACGGTATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCC 27CA10AG12
# IGBLASTN 2.2.29+
# Query: SRR765688.43118 HQP9GKT01AR4EN length=394|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=4
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 VH Yes In-frame No +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG (AG) GATATT CCTACGACCTCGATGGCAATTATGGTGG CTTTG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 24 2 0 92.3
CDR1-IMGT 47 70 24 16 8 0 66.7
FR2-IMGT 71 121 51 44 7 0 86.3
CDR2-IMGT 122 142 21 12 9 0 57.1
FR3-IMGT 143 267 125 97 17 11 77.6
CDR3-IMGT (germline) 268 274 7 7 0 0 100
Total N/A N/A 254 200 43 11 78.7
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.43118 IGHV4-59*02 78.74 254 43 1 11 21 274 50 292 2e-60 223 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCGTCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.43118 IGHV4-59*01 78.35 254 44 1 11 21 274 50 292 2e-59 219 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAG CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAG 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11
V SRR765688.43118 IGHV4-59*07 78.26 253 44 1 11 21 273 50 291 5e-59 218 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGA CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTATATCTATTACAGTGGGAGCACCAACTACAACCCC-----------TCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGA 18GC1AG7CT6GACT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA10
D SRR765688.43118 IGHD2-15*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.43118 IGHD2-2*01 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
D SRR765688.43118 IGHD2-2*02 100.00 8 0 0 0 273 280 1 8 2.9 16.4 AGGATATT AGGATATT 8
J SRR765688.43118 IGHJ4*02 93.18 44 3 0 0 309 352 5 48 1e-14 63.9 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23TC2GT10GT6
J SRR765688.43118 IGHJ4*01 90.91 44 4 0 0 309 352 5 48 3e-12 56.0 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 18GA4TC2GT10GT6
J SRR765688.43118 IGHJ4*03 88.64 44 5 0 0 309 352 5 48 7e-10 48.1 CTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG CTTTGACTACTGGGGCCAAGGGACCCTGGTCACCGTCTCCTCAG 18GA2AG1TC2GT10GT6
# IGBLASTN 2.2.29+
# Query: SRR765688.21808 HQP9GKT01A2WPP length=385|SEQORIENT=F,F,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR2,LR5|DUPCOUNT=9
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GTGAG GAATATAAGGCGCTCCGATAATACTGC TTATTAT GCCGAGTAT TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 55 35 33 2 0 94.3
CDR1-IMGT 56 79 24 19 5 0 79.2
FR2-IMGT 80 130 51 45 6 0 88.2
CDR2-IMGT 131 154 24 22 2 0 91.7
FR3-IMGT 155 268 114 98 16 0 86
CDR3-IMGT (germline) 269 273 5 4 1 0 80
Total N/A N/A 253 221 32 0 87.4
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.21808 IGHV5-51*01 87.35 253 32 0 0 21 273 41 293 2e-82 296 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG CCGGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.21808 IGHV5-51*03 87.25 251 32 0 0 23 273 43 293 2e-81 293 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
V SRR765688.21808 IGHV5-51*04 86.85 251 33 0 0 23 273 43 293 1e-80 290 GGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAG GGGGAGTCTCTGAAGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCGGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGATCATCTATCCTGGTGACTCTGATACCAGATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGCCCATCAGCACCGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAG 14AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA1TC2GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3
D SRR765688.21808 IGHD3-10*01 100.00 7 0 0 0 301 307 22 28 12 14.4 TTATTAT TTATTAT 7
D SRR765688.21808 IGHD3-10*02 100.00 7 0 0 0 301 307 21 27 12 14.4 TTATTAT TTATTAT 7
D SRR765688.21808 IGHD3-16*01 100.00 7 0 0 0 298 304 28 34 12 14.4 TGCTTAT TGCTTAT 7
J SRR765688.21808 IGHJ4*02 96.88 32 1 0 0 317 348 15 46 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.21808 IGHJ5*02 96.88 32 1 0 0 317 348 18 49 3e-12 56.0 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 16AT15
J SRR765688.21808 IGHJ1*01 93.75 32 2 0 0 317 348 19 50 7e-10 48.1 TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC TGGGGCCAGGGCACCCTGGTCACCGTCTCCTC 11AC4AT15
# IGBLASTN 2.2.29+
# Query: SRR765688.39792 HQP9GKT01C51DY length=373|SEQORIENT=F,RC,F|MID=MID54|CPRIMER=IgG-PCR|VPRIMER=LR3|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV7-4-1*02 IGHD6-25*01 IGHJ4*02 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
CGAGA CTCGACG GCAGCG ATAGG GACTT
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 62 42 38 4 0 90.5
CDR1-IMGT 63 86 24 21 3 0 87.5
FR2-IMGT 87 137 51 49 2 0 96.1
CDR2-IMGT 138 161 24 20 4 0 83.3
FR3-IMGT 162 275 114 103 11 0 90.4
CDR3-IMGT (germline) 276 281 6 6 0 0 100
Total N/A N/A 261 237 24 0 90.8
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.39792 IGHV7-4-1*02 90.80 261 24 0 0 21 281 34 294 1e-93 333 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG1GT18TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT28GA6GC10TC6AG4AG5CT3AG1TG15
V SRR765688.39792 IGHV7-4-1*01 90.42 261 25 0 0 21 281 34 294 9e-93 330 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCTGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG1GT18TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT28GA6GC5AT4TC6AG4AG5CT3AG1TG15
V SRR765688.39792 IGHV7-4-1*04 90.42 261 25 0 0 21 281 34 294 9e-93 330 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGA AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCATGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGA 4GA19AG11AG1GT18TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT24CT3GA6GC10TC6AG4AG5CT3AG1TG15
D SRR765688.39792 IGHD6-25*01 100.00 6 0 0 0 289 294 8 13 45 12.4 GCAGCG GCAGCG 6
D SRR765688.39792 IGHD3-22*01 100.00 5 0 0 0 294 298 11 15 178 10.4 GATAG GATAG 5
D SRR765688.39792 IGHD3-9*01 100.00 5 0 0 0 293 297 7 11 178 10.4 CGATA CGATA 5
J SRR765688.39792 IGHJ4*02 95.00 40 2 0 0 300 339 9 48 1e-14 63.9 GACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 4TA17AT17
J SRR765688.39792 IGHJ5*02 97.14 35 1 0 0 305 339 17 51 4e-14 61.9 CTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG CTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 17AT17
J SRR765688.39792 IGHJ4*01 92.50 40 3 0 0 300 339 9 48 3e-12 56.0 GACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GACTACTGGGGCCAAGGAACCCTGGTCACCGTCTCCTCAG 4TA9GA7AT17
# IGBLASTN 2.2.29+
# Query: SRR765688.39546 HQP9GKT01D90AI length=383|SEQORIENT=F,RC,F|MID=MID53|CPRIMER=IgG-PCR|VPRIMER=LR7|DUPCOUNT=2
# Database: database/human_igh_v database/human_igh_d database/human_igh_j
# Domain classification requested: imgt
# V-(D)-J rearrangement summary for query sequence (Top V gene match, Top D gene match, Top J gene match, Chain type, stop codon, V-J frame, Productive, Strand). Multiple equivalent top matches having the same score and percent identity, if present, are separated by a comma.
IGHV4-34*01,IGHV4-34*02 IGHD3-22*01,IGHD3-3*01,IGHD3-9*01 IGHJ1*01 VH No In-frame Yes +
# V-(D)-J junction details based on top germline gene matches (V end, V-D junction, D region, D-J junction, J start). Note that possible overlapping nucleotides at VDJ junction (i.e, nucleotides that could be assigned to either rearranging gene) are indicated in parentheses (i.e., (TACT)) but are not included under the V, D, or J gene itself
GCGAG GGTGAGCGTCCGATTGGACACTACGGGCTA TTATTAC GGGTCCCTAGATTAT TGGGG
# Alignment summary between query and top germline V gene hit (from, to, length, matches, mismatches, gaps, percent identity)
FR1-IMGT 21 46 26 24 2 0 92.3
CDR1-IMGT 47 70 24 19 5 0 79.2
FR2-IMGT 71 121 51 44 7 0 86.3
CDR2-IMGT 122 142 21 16 5 0 76.2
FR3-IMGT 143 256 114 99 15 0 86.8
CDR3-IMGT (germline) 257 261 5 5 0 0 100
Total N/A N/A 241 207 34 0 85.9
# Hit table (the first field indicates the chain type of the hit)
# Fields: query id, subject id, % identity, alignment length, mismatches, gap opens, gaps, q. start, q. end, s. start, s. end, evalue, bit score, query seq, subject seq, BTOP
# 9 hits found
V SRR765688.39546 IGHV4-34*01 85.89 241 34 0 0 21 261 50 290 6e-75 271 CCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 18TC4CT6CG3GC7AG3TC2TC4CGAC8GC3TC4TA14AG8AG6CA2GC8GAAG2TC20TACG5CACG7CA23AG9CAGA4GACG19TA3GC1AG1TG2CT11
V SRR765688.39546 IGHV4-34*02 85.89 241 34 0 0 21 261 50 290 6e-75 271 CCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 18TC4CT6CG3GC7AG3TC2TC4CGAC8GC3TC4TA14AG8AG6CA2GC8GAAG2TC20TACG5CACG7CA23AG9CAGA4GACG19TA3GC1AG1TG2CT11
V SRR765688.39546 IGHV4-34*12 85.48 241 35 0 0 21 261 50 290 5e-74 268 CCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAG CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGGAAATCATTCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAG 18TC4CT6CG3GC7AG3TC2TC4CGAC8GC3TC4TA14AG8AG6CAAT1GC8GAAG2TC20TACG5CACG7CA23AG9CAGA4GACG19TA3GC1AG1TG2CT11
D SRR765688.39546 IGHD3-22*01 100.00 7 0 0 0 292 298 22 28 11 14.4 TTATTAC TTATTAC 7
D SRR765688.39546 IGHD3-3*01 100.00 7 0 0 0 293 299 2 8 11 14.4 TATTACG TATTACG 7
D SRR765688.39546 IGHD3-9*01 100.00 7 0 0 0 293 299 2 8 11 14.4 TATTACG TATTACG 7
J SRR765688.39546 IGHJ1*01 100.00 11 0 0 0 314 324 19 29 0.039 22.3 TGGGGCCAGGG TGGGGCCAGGG 11
J SRR765688.39546 IGHJ3*01 100.00 8 0 0 0 314 321 17 24 2.4 16.4 TGGGGCCA TGGGGCCA 8
J SRR765688.39546 IGHJ3*02 100.00 8 0 0 0 314 321 17 24 2.4 16.4 TGGGGCCA TGGGGCCA 8
# BLAST processed 100 queries
Raw
SRR765688_db-pass.txt
SEQUENCE_ID SEQUENCE_INPUT FUNCTIONAL IN_FRAME STOP MUTATED_INVARIANT INDELS V_CALL D_CALL J_CALL SEQUENCE_VDJ SEQUENCE_IMGT V_SEQ_START V_SEQ_LENGTH V_GERM_START_VDJ V_GERM_LENGTH_VDJ V_GERM_START_IMGT V_GERM_LENGTH_IMGT NP1_LENGTH D_SEQ_START D_SEQ_LENGTH D_GERM_START D_GERM_LENGTH NP2_LENGTH J_SEQ_START J_SEQ_LENGTH J_GERM_START J_GERM_LENGTH JUNCTION_LENGTH JUNCTION V_SCORE V_IDENTITY V_EVALUE V_BTOP J_SCORE J_IDENTITY J_EVALUE J_BTOP HMM_SCORE FWR1_IMGT FWR2_IMGT FWR3_IMGT FWR4_IMGT CDR1_IMGT CDR2_IMGT CDR3_IMGT SEQORIENT MID CPRIMER VPRIMER DUPCOUNT
SRR765688.30659 HQP9GKT01B1E02 length=380 NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTCGGCCTCCAC T T F F IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTC............ACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATC......ACTGGTAACCCAACTTACGCCCAGGCCTTCACA...GGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC 23 257 36 257 1 316 17 297 6 8 6 8 311 33 14 33 42 TGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGG 330.0 0.9105 9e-93 22AG24TCTC7AGAC3TG1AT4GA44AGCG10TA6TG8TG2CT7CG21TC15AG35AG4CG7GC1AG7TC3TG3 50.1 0.9394 2e-10 13GA8GA10 ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCT ATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATAC ACTTACGCCCAGGCCTTCACA...GGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGT TGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTC GGATACATTTTC............ACTAAATATTCA ATCAACACCATC......ACTGGTAACCCA TCGACCCTCCAACCGCGTATTGCAGCACCTGAATTC F,RC,F MID54 IgG-PCR LR3 3
SRR765688.38189 HQP9GKT01B1F8D length=357 NNNNNNNNNNNNNNNNNNNCTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC F F F F IGHV6-1*01,IGHV6-1*02 IGHD3-16*01,IGHD3-16*02,IGHD3-3*01 IGHJ4*02 CTGTGACATCTCCGGGGAGAGTATCTCTAACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCCGAGTGGAATTACGATTATGCACCCTCTCTGAGAAGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .................................................................CTGTGACATCTCCGGGGAGAGTATCTCT......AACACCAATGTTGGCTGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGGACATTCTACAGGTCC...GAGTGGAATTACGATTATGCACCCTCTCTGAGA...AGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 20 239 63 239 1 316 9 268 6 15 6 9 283 41 8 41 41 TGTGCAAAAACATCCTTTTGGGACGCGGGCATGACTATTGG 290.0 0.887 1e-80 5AC12GC3AG6AG2CA2AG2TC2GCCT4CA27TC22TA10GA5AT2TA1CT9CGCTCA3CG3GA9TA14TC7GA23GA27AG5TC7 73.8 0.9756 1e-17 6TC34 .................................................................CTGTGACATCTCC TGGACCTGGATCAGGCAGTCCCCATCGAGAGGTCTTGAGTGGCTGGGAAGG GATTATGCACCCTCTCTGAGA...AGTCGAATTACCATCAACCCAGATACATCCAGGAACCAGTTCTCCCTGCAGCTGAGCTCTGTGACTCCCGAGGACACGGCTGTATATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGGGAGAGTATCTCT......AACACCAATGTTGGC ACATTCTACAGGTCC...GAGTGGAATTAC GCAAAAACATCCTTTTGGGACGCGGGCATGACTAT F,F,F MID53 IgG-PCR LR1 2
SRR765688.23676 HQP9GKT01CBCVC length=380 NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTC............ACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATC......AGTGGAAACCCAACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23 257 36 257 1 316 12 292 11 3 11 8 311 35 14 35 42 TGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGG 336.0 0.9183 1e-94 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC41ACGA8AG14AG1AG9TG3 69.9 1.0 2e-16 35 ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGG ACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGAGGCCTAAAGACTGAGGACACTGCCATATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACCTTC............ACTAAATATCCA ATCAACACCATC......AGTGGAAACCCA TCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTC F,RC,F MID54 IgG-PCR LR3 5
SRR765688.44566 HQP9GKT01BUA3B length=393 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC F T T T IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGG-CCAGGGAATCCGGGTCACCGTCGCCTCAG ....................................................CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCC............GCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGT.........TGGGGCAGCAAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGG-CCAGGGAATCCGGGTCACCGTCGCCTCAG 21 254 50 243 1 319 0 275 6 3 6 28 309 43 5 44 57 TGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGG 223.0 0.7874 2e-60 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11 48.1 0.9091 7e-10 14-G8TC2GT10GT6 ....................................................CCCTGTCCCTCACCTGCAGTATCTCT TGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTT AAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGT TGGG-CCAGGGAATCCGGGTCACCGTCGCCTCAG GGCGGCTCCGCC............GCCCACTACTAC GCCTATGTAGGT.........TGGGGCAGC GCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTAC F,RC,F MID54 IgG-PCR LR7 2
SRR765688.49541 HQP9GKT01ESY9C length=377 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG ....................................AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTC............AGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAAT......ACTGGGAACCCAACATATGCCCAGGCCTTCACA...GGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG 21 263 34 263 1 320 11 295 6 7 6 0 301 42 7 42 39 TGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGG 324.0 0.8935 7e-91 12AG23AG1CT9TA6GC2AG21GA32GA11TC2TC14AG10CG12AG3AG8CT16TC3AG8GC6AG2AG1TC1GA8AG2TC6AG1AG10GCAG5 67.9 0.9523999999999999 7e-16 7TC21GA12 ....................................AAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCT ATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGG ACATATGCCCAGGCCTTCACA...GGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGT TGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAG GGATACTCCTTC............AGTAACTATGCT ATCAACACTAAT......ACTGGGAACCCA GGAAGAGAGAGTCTCGGCCACTGGATTGACTAT F,F,F MID54 IgG-PCR LR3 3
SRR765688.45233 HQP9GKT01BVKNW length=371 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCGGCCTCCAC T T F F IGHV4-59*01,IGHV4-59*07 IGHD7-27*01 IGHJ4*02 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATCAGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAATGGGACCACCACCTCTAACCCCTCCCTCAACAGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC ....................................................CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCCCCATC............AGTGGTCACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATATATCTATTACAAT.........GGGACCACCACCTCTAACCCCTCCCTCAAC...AGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 21 240 50 240 1 316 4 265 6 6 6 22 293 35 12 35 45 TGTGCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTACTGG 319.0 0.925 2e-89 32CT8GA2CT52AG13AG5CG5CA2CATC14CG14GA2CA12CA4TC14CGGC2GA1GC40 69.9 1.0 2e-16 35 ....................................................CCCTGTCCCTCACCTGCACTGTCTCT TGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGATAT ACCTCTAACCCCTCCCTCAAC...AGTCGAGTCACCATGTCCGTAGACACGTCCCAGAATCAGTTCTCCCTGAACGTGGGGTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC GGTGGCCCCATC............AGTGGTCACTAC ATCTATTACAAT.........GGGACCACC GCGAAGAATGGGGACACCACCTCGCCCGGATTTAGGTAC F,RC,F MID54 IgG-PCR LR7 2
SRR765688.17591 HQP9GKT01CLJHN length=395 NNNNNNNNNNNNNNNNNNCCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC T T F F IGHV4-30-4*01 IGHD2-8*01 IGHJ6*02,IGHJ6*04 CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC ..........................................CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGC......ACTGGGGATTACTACTGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCTATAACAGT.........GGGAGCACCTACTACAACCCGTCCCTCAAG...AGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGTGCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC 19 260 40 260 1 320 3 282 9 7 9 24 315 28 8 28 75 TGTGCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGGGGC 363.0 0.9462 1e-102 6GC45CG3GT27TC38AT43CT1TC3GA11AG4GA37AG5GA5AG1AG17 40.1 0.9286 2e-07 13GACT13 ..........................................CCTTCAGAGACCCTGTCCCTCACCTGCACTGTCTCT TGGAGTTGGATCCGCCAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGGTAC TACTACAACCCGTCCCTCAAG...AGTCGAGTCATCATGTCAGTAGACACATCCAGGAACCAGTTCTCCCTGAAGCTGAGCTCTGTGACTGCCACAGACGCGGCCATATATTACTGT GGC GGTGGCTCCATCAGC......ACTGGGGATTACTAC ATCTATAACAGT.........GGGAGCACC GCCAGAGATGGTTGTACTAAGGGTTTATGCAGAACGAGGTCGGCCTACTACTACGGTGCGGACGTCTGG F,F,F MID54 IgG-PCR LR10 2
SRR765688.43976 HQP9GKT01CAKGO length=383 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F T IGHV4-39*01 IGHD1-7*01 IGHJ4*02 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGC......AGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGT.........GGGAGTACCTACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 247 50 250 1 320 14 282 8 10 8 13 303 45 4 45 60 TGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGG 341.0 0.9440000000000001 5e-96 17GA12AG20GA50CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17 73.8 0.9556 1e-17 9CATC34 ....................................................CCCTGTCCCTCACCTGCGCTGTCTCT TGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGT TACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGACTCCATCAGC......AGTAGTAGTTGCTAC ATCTAT---AGT.........GGGAGTACC GCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCT F,RC,F MID54 IgA-PCR LR7 14
SRR765688.49474 HQP9GKT01DTUWB length=384 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCACGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV4-39*01 IGHD1-7*01 IGHJ4*02 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGC......AGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGT.........GGGAGTACCTACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 248 50 250 1 320 14 283 8 10 8 13 304 45 4 45 60 TGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGG 333.0 0.9401999999999999 1e-93 17GA12AG20GA21C-29CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17 73.8 0.9556 1e-17 9CATC34 ....................................................CCCTGTCCCTCACCTGCGCTGTCTCT TGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGT TACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGACTCCATCAGC......AGTAGTAGTTGCTAC ATCTAT---AGT.........GGGAGTACC GCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCT F,RC,F MID54 IgA-PCR LR7 2
SRR765688.34997 HQP9GKT01BF6LJ length=395 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCAACCTCCAC T T F F IGHV7-4-1*02 IGHD6-13*01,IGHD6-6*01 IGHJ5*01,IGHJ5*02 AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTCCGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAGACTGGAAATCCAACGTATGCCCAGGGCTACACAGGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA ....................................AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCTGGATACATTTTC............CGTGATTATGCTATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCGAG......ACTGGAAATCCAACGTATGCCCAGGGCTACACA...GGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGTGCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCCTGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA 21 262 34 262 1 319 14 297 8 10 8 12 317 43 8 43 57 TGTGCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCCTGG 316.0 0.8855 2e-88 18AG2GA1AGAG24TCTC3CAGC1GAAGTC8AG11CA45GA1GC5AG2TC19AT27AC10CG6TA1TA4AC6AGTC1AG7AG1GT2AG16TA11 61.9 0.9301999999999999 5e-14 18GA7GT5TC10 ....................................AAGAAGCCTGGGGCCTCAATGGAAATTTCCTGCAAGGCTTCT ATAAATTGGGTGCGCCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACGTATGCCCAGGGCTACACA...GGACGGTTTGTCTTCTCCTTGGAAACCTCTGTCACCACGGCTTTTCTGAAGATCAATAACCTAAAGACGGAAGACACTGCCGTGTATTTCTGT TGGGGCCAGGGAACCCGGGTCATCGTCTCCTCA GGATACATTTTC............CGTGATTATGCT ATCAACACCGAG......ACTGGAAATCCA GCGAGAGCTTCCTGTAGCATTAGCAGCTGCCATGCCCAGCAGTTCGACTCC F,F,F MID53 IgG-PCR LR3 2
SRR765688.33811 HQP9GKT01AZ9Y4 length=404 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD3-9*01 IGHJ6*02 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTC............ACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGA......ACTGGGGACCCAACGTATGCCCAGGCCTTCACA...GGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA 21 263 34 263 1 320 7 291 8 10 8 23 322 47 16 47 66 TGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGG 330.0 0.9011 1e-92 26CT16CG5GCTC6GAAGGC6CA3CA24GA6CG23GAGAAC6GA18CG12TG11GC1CT21TA8CG6GA1TC6CG16TG5TC11 77.8 0.9573999999999999 8e-19 5CA23GA17 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCT CTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGG ACGTATGCCCAGGCCTTCACA...GGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGT TGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA GCATACAGTTTC............ACTGAGTATGCT ATCAACACCGGA......ACTGGGGACCCA GCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTC F,RC,F MID54 IgG-PCR LR3 3
SRR765688.40396 HQP9GKT01DTRVH length=361 NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*01,IGHV4-59*02,IGHV4-59*07 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG .................................................................CTGCAGTGTCTCTGGCGGCGGCTCC............GGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGG.........GCCAATTTCAAGTACAACCCCTCCGTGAAG...AATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG 20 228 63 228 1 317 1 249 7 2 7 29 285 43 6 43 57 TGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGG 204.0 0.7851 8e-55 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12TC11AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14 61.9 0.9301999999999999 4e-14 8TC13TC2GT17 .................................................................CTGCAGTGTCTCT TGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTC AAGTACAACCCCTCCGTGAAG...AATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGT TGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG GGCGGCGGCTCC............GGCCACTATTAT ATCTATGATGGG.........GCCAATTTC GCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTAT F,RC,F MID54 IgA-PCR LR1 3
SRR765688.38176 HQP9GKT01CL3CG length=383 NNNNNNNNNNNNNNNNNNNNCCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*01 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACCGCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGTGGGGTCACCAAGTACAACCCCTCCCTCAAGAGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG ....................................................CCCTGTCTCTCACCTGCAGTATCTCTGGCGCCTCCACC............GCCCATTACTATTGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTTATCTATGAAGGT.........GGGGTCACCAAGTACAACCCCTCCCTCAAG...AGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTACTGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG 21 243 50 243 1 319 0 264 6 3 6 29 299 43 6 43 57 TGTGCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTACTGG 258.0 0.8395 3e-71 7TC10GC1AG7CT1CG5CT1GACGCTCAAG6TC4AGTC12TC28TG1TA7GT1ACGA5GATG6GC23CA8GA8TCTA6GA1GA2AC4GC7GAAG4CG3CT8CT14CG2CT1TA11 61.9 0.9301999999999999 4e-14 22TC2GT4TA12 ....................................................CCCTGTCTCTCACCTGCAGTATCTCT TGGAATTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGGTTTT AAGTACAACCCCTCCCTCAAG...AGTCGCGTCACCATGTCAGTAGATTCGTCCAGGGACAAGTTGTCCCTGAGACTGACCTCCGTGACCGCCGCGGACACGGCCGTCTACTTCTGT TGGGGCCAGGGAATCCGGGTCTCCGTCTCCTCAG GGCGCCTCCACC............GCCCATTACTAT ATCTATGAAGGT.........GGGGTCACC GCGAGAGGATATTCCTATAATCTGGATGGCAATTATGGTGGTTTTGACTAC F,RC,F MID54 IgA-PCR LR7 2
SRR765688.36681 HQP9GKT01B98GC length=382 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD3-22*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTCATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAACACTGGAAGTCCGGCCTATGCCCGGGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCTGGATACCCCTTC............ATTAACTATGCAATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGAAGTCCGGCCTATGCCCGGGGCTTCACA...GGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGTGCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG 21 263 34 263 1 320 10 294 13 14 13 0 307 42 7 42 45 TGTGCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCACTGG 343.0 0.9163 2e-96 34GA13CA6TC2AG6AT2AGCA18GC9TG35AG1GATC2GAGA1CG7GA12GA53TCGA17TA3GC1TG19 69.9 0.9534999999999999 2e-16 6CT14GA21 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAGGGCTTCT ATACATTGGGTGCGACAGGCCCGTGGACAAGGTCTTGAGTGGATGGGATGG GCCTATGCCCGGGGCTTCACA...GGGCGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGTGGCCTAAAGGCTGAGGACTCTGGCTTGTATTACTGT TGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG GGATACCCCTTC............ATTAACTATGCA ATCAACACCAAC......ACTGGAAGTCCG GCGAGAGAAGAACGTCGAAGTAGTGGTTATTTTGACCAC F,RC,F MID53 IgG-PCR LR3 13
SRR765688.26204 HQP9GKT01AGSP2 length=365 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV6-1*01,IGHV6-1*02 IGHD1-1*01,IGHD1-1*01 IGHJ5*02 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......AGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATTATACAGGTCC...AAGTGGTATAACAATTATGCAGTATCTGTGAAA...AGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 20 241 63 242 1 319 8 269 7 4 7 11 287 43 9 43 48 TGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGG 322.0 0.9339 2e-90 35CG2ACAT49-G8TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24 85.7 1.0 3e-21 43 .................................................................CTGTGCCATCTCC TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGG AATTATGCAGTATCTGTGAAA...AGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGGGACAGTGTCTCT......AGCAACACTGAAGCT ACATTATACAGGTCC...AAGTGGTATAAC GCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCC F,RC,F MID53 IgA-PCR LR1 2
SRR765688.31319 HQP9GKT01B1IO7 length=373 NNNNNNNNNNNNNNNNNNNNCAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .....................................AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTC............ACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGC......ACCGGGACGCCAACATATGCCCAGGACTTCACA...GGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 22 260 35 260 1 318 0 282 5 8 5 13 300 40 9 40 36 TGTGTGAGACTGGACGGGACCGACTCGGACTACTGG 329.0 0.9037999999999999 3e-92 23AG24TC7CA1TC3TG1CT2AG51GA6GA3CT4CAGC5AG10AG12AG20GC8TC11AG7AGTC1AG7TG4CG11CG10TC4 79.8 1.0 2e-19 40 .....................................AGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCT ATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACATATGCCCAGGACTTCACA...GGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACATCTTC............ACTCGTTATTCC ATCGACACCAGC......ACCGGGACGCCA GTGAGACTGGACGGGACCGACTCGGACTAC F,RC,F MID54 IgG-PCR LR3 2
SRR765688.20699 HQP9GKT01DRRB0 length=386 NNNNNNNNNNNNNNNNNNTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGATGGACCCAACTCCCCTCGACTACTGGGGCCTGGGAACCCTGGTCACCGTCGCTTCAGCCTCCAC F F F F IGHV3-30-3*01 IGHD5-24*01 IGHJ4*02 TGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTCAGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGATGGAAGCGATAAATATTATGCAGAATCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGATGGACCCAACTCCCCTCGACTACTGGGGCCTGGGAACCCTGGTCACCGTC ....................TGGGGGA...GGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCTGGATTCAACTTC............AGTCCCTATGCTATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATTTTGTCATCTGAT......GGAAGCGATAAATATTATGCAGAATCCGTGAAG...GGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGTTCGAGAGATGGACCCAACTCCCCTCGACTACTGGGGCCTGGGAACCCTGGTCACCGTC 19 274 21 274 1 318 0 293 5 6 5 14 312 33 9 33 37 TGTTCGAGAGATGGACCCAACTCCCCTCGACTACTGG 344.0 0.9015000000000001 6e-97 47TC2TCTC10AC7CACG16CG22TG3CG11AG2TA1GA4CA10GA7TC2TC5AC47CG6TC1AG18CG3TA6AG1GC3AG5TC3TG5 58.0 0.9697 7e-13 13TA19 ....................TGGGGGA...GGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGTAGTTTCT ATGCACTGGCTCCGCCAGGCTCCAGGCAAGGGTCTGCAGTGGGTGGCAATT TATTATGCAGAATCCGTGAAG...GGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATTTACAAATGAACAGCCTGAGACCTGTGGACACAGGTGTATATTATTGT TGGGGCCTGGGAACCCTGGTCACCGTC GGATTCAACTTC............AGTCCCTATGCT TTGTCATCTGAT......GGAAGCGATAAA TCGAGAGATGGACCCAACTCCCCTCGACTAC F,F,F MID54 IgG-PCR LR11 2
SRR765688.48796 HQP9GKT01B94FW length=376 NNNNNNNNNNNNNNNNNNACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCAGCCTCCAC T T F F IGHV3-33*01 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01 IGHJ6*02 ACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCA ....................................................................................ACCATC............AGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGAT......GGAAGTAATGAATACTATGCAGACTCCGTGAAG...GGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCA 19 215 82 215 1 320 33 267 8 15 8 5 280 42 21 42 69 TGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGG 305.0 0.9534999999999999 3e-85 3AT86GA48CA3ACCA8GC11CA4TC26TG5TC11 75.8 0.9762000000000001 3e-18 35GC6 .............................................................................. ATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTT TACTATGCAGACTCCGTGAAG...GGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGT TGGGGCCAAGGGACCACGGTCACCGTGTCCTCA ......ACCATC............AGTAGCTATGGC ATATGGTATGAT......GGAAGTAATGAA GCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTC F,RC,F MID53 IgG-PCR LR11 2
SRR765688.41848 HQP9GKT01C3DX5 length=387 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCCGCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD2-15*01 IGHJ5*02 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCC ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTC............ACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAG......ACTGGGAACCCAACATATGTCCAGGACTTCACA...GGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCC 21 263 34 263 1 320 11 295 9 10 9 3 307 44 4 44 51 TGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGG 371.0 0.9506 6e-105 24AG13CT9CA10TC68GC14AG4TC5AG25CT29GC14GA4CT19TA12 79.8 0.9773000000000001 2e-19 31AG12 ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACATATGTCCAGGACTTCACA...GGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGT TGGGGCCAGGGAACCCTAGTCACCGTCTCC GGATACCCCTTC............ACTAGTTATGCT ATCAACACCAAG......ACTGGGAACCCA GCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCC F,RC,F MID53 IgA-PCR LR3 2
SRR765688.23863 HQP9GKT01BPQLA length=359 NNNNNNNNNNNNNNNNNNNCTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCGCAACCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD3-10*01,IGHD3-10*02 IGHJ4*02 CTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC .................................................................CTGCGTCATCTCCGGAGACAACGTCTCT......AGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCC...AAGTGGTATAATGATTATGCAATTTCTATGGAA...AGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCC 20 243 63 243 1 320 6 269 5 14 5 3 277 44 1 44 42 TGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGG 318.0 0.9177 5e-89 3CT1TC9AG4AGCT9GA3CG94AG1TA3AG2GA12TCTC5GC20CG12TC4GC3CG22CG5TC11 71.9 0.9545 4e-17 6AT4CT32 .................................................................CTGCGTCATCTCC TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG GATTATGCAATTTCTATGGAA...AGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCC GGAGACAACGTCTCT......AGCGACACTGCTGCT ACATACTACAGGTCC...AAGTGGTATAAT GCAAGAGACAAGAGTCGGGATGACTACTATGACCAC F,F,F MID54 IgG-PCR LR1 2
SRR765688.17127 HQP9GKT01CQJSD length=377 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCCCG T T F F IGHV7-4-1*02 None IGHJ3*01 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTC............ACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAAC......ACTGGTAACCCAACATATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG 21 263 34 263 1 320 12 296 47 4 47 39 TGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGG 358.0 0.9354 3e-101 57GACG4TG44CA6AGTG6CA1AC8TG8AG53TC8CTGC10TC6GA19AG9AG7 85.7 0.9787 3e-21 12GC34 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATAT ACATATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGT TGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG GGATACACCTTC............ACTGCCTATTCT ATCAACCCAAAC......ACTGGTAACCCA ACGAGAGACAGACATTCATGTGCTTTTGATGTG F,RC,F MID54 IgA-PCR LR3 4
SRR765688.32432 HQP9GKT01CZ7DE length=367 NNNNNNNNNNNNNNNNNNNNGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG T T F F IGHV1-69*01,IGHV1-69*12,IGHV1-69*13 IGHD6-13*01 IGHJ6*02 GTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC ............................................................GTCTCCTGCAAGGCTTCTGGAGGCACGTTC............AGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATC......GTTGGTACAGCAAAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC 21 238 58 238 1 319 7 266 8 10 8 2 276 51 9 51 51 TGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGG 313.0 0.9201999999999999 1e-87 26GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16 93.7 0.9804 1e-23 38AG12 ............................................................GTCTCCTGCAAGGCTTCT ATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGG AAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGT TGGGGCCAAGGGACCACAGTCACCGTCTCC GGAGGCACGTTC............AGCCACTATGCT ATCATCCCTATC......GTTGGTACAGCA GCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTC F,RC,F MID54 IgA-PCR LR9 2
SRR765688.20304 HQP9GKT01AMKZ5 length=386 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCTGCATCCCCG T T F F IGHV7-4-1*02 IGHD2-21*02 IGHJ5*02 AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTCACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAATAATGGCAATCCAACATATGCCCCGGGCCTCACAGGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC ....................................AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATATAGTTTC............ACCAATTTTGCTATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGGATCAATACCAAT......AATGGCAATCCAACATATGCCCCGGGCCTCACA...GGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGTGCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC 21 263 34 263 1 320 22 306 7 15 7 0 313 37 13 37 48 TGTGCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTCTGG 290.0 0.8517 1e-80 12AG13CT20TC1GCTC5CT1AGTC1TA23TC12AG9CA13TC5TC1AC3CG2TC5AG7CA4CT10AGAT16ACGA5CG3AG6CA1TA3AG8TC1AG3GACA2AG4AG2TC6AG1TG4TA12 67.9 0.9737 7e-16 4TC33 ....................................AAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCAAGGCTTCT ATGAATTGGGTGCGACAGGTCCCTGGACAAGGACTTGAGTGGCTGGGATGG ACATATGCCCCGGGCCTCACA...GGACGAATTGTCTTCTCCTTGGAAGCCTCTCTCAACACGGCCTTTCTACAGATCAGTAACCTGCAGACTGAAGATACTGCCATTTATTTCTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTC GGATATAGTTTC............ACCAATTTTGCT ATCAATACCAAT......AATGGCAATCCA GCGAGAGAGGGACTGTGGGACGGCAGGGTTGTGACTGACCTC F,RC,F MID54 IgA-PCR LR3 4
SRR765688.47093 HQP9GKT01BTC88 length=384 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTC............ACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAAACCGACGTATGCCCAGGGCTTCACA...GGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG 21 263 34 263 1 320 12 296 9 9 9 6 311 41 8 41 48 TGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGG 352.0 0.9278 3e-99 22GA10GA7CT14CTTACG4AG4AGCA67AC2GA26AG49AG2AG1GC2CA13CT3AG1TG17 73.8 0.9756 1e-17 24AG16 ....................................AAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCC ATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACGTATGCCCAGGGCTTCACA...GGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGT TGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAG GGATACACCTTC............ACCTCCTATACT ATCAACACCAAC......ACTGGGAAACCG GCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTAC F,F,F MID54 IgA-PCR LR3 3
SRR765688.46353 HQP9GKT01CZULB length=366 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV6-1*01,IGHV6-1*02 IGHD1-1*01,IGHD1-1*01 IGHJ5*02 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......AGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCC...AAGTGGTATAACAATTATGCAGTATCTGTGAAA...AGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 20 242 63 242 1 319 8 270 7 4 7 11 288 43 9 43 48 TGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGG 332.0 0.938 3e-93 35CG2ACAT58TAAC20CTAG22CT15GC6TC2AC26GAGC14CG3GA24 85.7 1.0 3e-21 43 .................................................................CTGTGCCATCTCC TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG AATTATGCAGTATCTGTGAAA...AGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGGGACAGTGTCTCT......AGCAACACTGAAGCT ACATTATACAGGTCC...AAGTGGTATAAC GCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCC F,RC,F MID53 IgA-PCR LR1 2
SRR765688.49658 HQP9GKT01D85J4 length=395 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC F T T T IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGCTGGGGCAGCAAGTCCGACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG ....................................................CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCC............GCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGC.........TGGGGCAGCAAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG 21 254 50 243 1 319 0 275 6 3 6 28 309 44 5 44 57 TGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGG 219.0 0.7835 2e-59 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA1CTTG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11 63.9 0.9318000000000001 1e-14 23TC2GT10GT6 ....................................................CCCTGTCCCTCACCTGCAGTATCTCT TGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTT AAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGT TGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG GGCGGCTCCGCC............GCCCACTACTAC GCCTATGTAGGC.........TGGGGCAGC GCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTAC F,RC,F MID54 IgG-PCR LR7 2
SRR765688.31392 HQP9GKT01BBVPS length=368 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02,IGHJ5*02 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTT............TCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATC......ACTGGGAACCCAACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 262 34 262 1 319 0 283 3 3 3 13 299 35 14 35 30 TGTGCGAGAGGATTCGACACCGGCCTCTGG 369.0 0.9504 2e-104 38CT11AC2TCTA3CG1CT3TC3AG39GA18TA64AG3CG27GA37 69.9 1.0 2e-16 35 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCT ATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGG ACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACATTT............TCTACCCATGTT ATCAACACCATC......ACTGGGAACCCA GCGAGAGGATTCGACACCGGCCTC F,RC,F MID53 IgA-PCR LR3 7
SRR765688.11728 HQP9GKT01AL5DZ length=389 NNNNNNNNNNNNNNNNNNNNAAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC T T F F IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC .......................................AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTT............GACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAA......GACTCTGATACCAGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC 21 257 37 257 1 317 27 305 7 22 7 9 321 32 15 32 54 TGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGG 302.0 0.8755 3e-84 20AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3 56.0 0.9688 3e-12 16AT15 .......................................AAGCCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCT CTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTC AGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC GGATACAACTTT............GACACCTATTGG ATCTATCCTGAA......GACTCTGATACC GTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTAT F,F,F MID54 IgG-PCR LR9 2
SRR765688.21411 HQP9GKT01CV52A length=362 NNNNNNNNNNNNNNNNNNNNNGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG T T F F IGHV1-46*01 IGHD1-1*01,IGHD1-20*01,IGHD2-21*01 IGHJ6*02 GTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA ............................................................GTTTCCTGTAAGGCATCTGGATACACCTTC............ACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGT......GGTGGTAGCACAAGCTACGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA 22 239 58 239 1 320 8 269 5 13 5 0 274 48 15 48 42 TGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGG 371.0 0.9958 5e-105 8TC230 91.7 0.98 5e-23 42CT7 ............................................................GTTTCCTGTAAGGCATCT ATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATA AGCTACGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGT TGGGGCCAAGGGACCACGGTCACCGCCTCCTCA GGATACACCTTC............ACCAGCTACTAT ATCAACCCTAGT......GGTGGTAGCACA GCGAGAGATTCCTTGCACGACTACGGTATGGACGTC F,RC,F MID54 IgA-PCR LR9 3
SRR765688.46885 HQP9GKT01B8Z8I length=380 NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTC............ACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATC......AGTGGAAACCCAACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23 257 36 257 1 316 12 292 11 3 11 8 311 35 14 35 42 TGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGG 339.0 0.9222 1e-95 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC42GA8AG14AG1AG9TG3 69.9 1.0 2e-16 35 ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGG ACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACCTTC............ACTAAATATCCA ATCAACACCATC......AGTGGAAACCCA TCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTC F,RC,F MID54 IgG-PCR LR3 7
SRR765688.15716 HQP9GKT01BPKZR length=374 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGAATGGACTACACTCATTACCATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD4-11*01,IGHD4-4*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTCACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAACACTGGGAAGTCTTCGTACGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGAATGGACTACACTCATTACCATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCTGGATACATTCTC............ACTAATTATGCTATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGGATCGACACCAAC......ACTGGGAAGTCTTCGTACGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGTGCGAGAATGGACTACACTCATTACCATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 261 34 261 1 318 3 285 7 2 7 11 303 37 12 37 36 TGTGCGAGAATGGACTACACTCATTACCATTACTGG 333.0 0.9079999999999999 1e-93 26GT22TCTCCT6AGTC15CG23TC10AG9GA16GCTC1TATA4CT40GC9AC19AG1GA5GA2AG14AG1TG5TC9 73.8 1.0 1e-17 37 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTGTCCTGCAAGGCTTCT ATGAATTGGCTGCGACAGGCCCCTGGACAAGGGTTTGAGTGGATAGGATGG TCGTACGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACAGCTCTGTCAGAACGGCATATCTGCAGATCAACGGCCTAGAGACTGAGGACACTGCCATTTATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACATTCTC............ACTAATTATGCT ATCGACACCAAC......ACTGGGAAGTCT GCGAGAATGGACTACACTCATTACCATTAC F,RC,F MID53 IgG-PCR LR3 2
SRR765688.28724 HQP9GKT01CM7HO length=385 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG T T F F IGHV1-69*01,IGHV1-69*12,IGHV1-69D*01 IGHD6-13*01 IGHJ6*02 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC .......................................AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTC............AGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATC......GTTGGTACAGCAAAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC 21 259 37 259 1 319 7 287 8 10 8 2 297 51 9 51 51 TGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGG 346.0 0.9266 2e-97 47GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16 93.7 0.9804 1e-23 38AG12 .......................................AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCT ATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGG AAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGT TGGGGCCAAGGGACCACAGTCACCGTCTCC GGAGGCACGTTC............AGCCACTATGCT ATCATCCCTATC......GTTGGTACAGCA GCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTC F,F,F MID54 IgA-PCR LR9 3
SRR765688.35930 HQP9GKT01CJLTL length=406 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC F F T F IGHV1-18*04 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01,IGHD3-10*01 IGHJ6*02 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTCACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTACAATGGTGACACAAATTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA .......................................AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACAGCTTC............ACCACTTACAGTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGTTTAC......AATGGTGACACAAATTATGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGTGCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 21 258 37 258 1 318 0 279 4 4 4 53 336 35 28 35 71 TGTGCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTCTGG 363.0 0.9495999999999999 1e-102 41AT4GC3CT4CGTC3AG6CG53TC10GA7TC12TC39CG53TA10 69.9 1.0 2e-16 35 .......................................AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCT ATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG AATTATGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCACAGACACATCCACGACCACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTTCTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGATACAGCTTC............ACCACTTACAGT ATCAGCGTTTAC......AATGGTGACACA GCGAGAATATCGGAGAAGATTATAATTAAACGAAGACTCGGCCCTATTATTACACTTTGAATCTC F,F,F MID54 IgG-PCR LR9 2
SRR765688.30106 HQP9GKT01CBSGA length=373 NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGACTTGACGGCAGCGATAGGGACTTTTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD3-3*01,IGHD3-3*02,IGHD3/OR15-3a*01 IGHJ4*02,IGHJ5*02 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTCACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTACACTGGAAGCCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGACTTGACGGCAGCGATAGGGACTTTTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCTGGATACACCTTC............ACTTCGTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCTAC......ACTGGAAGCCCAACCTATGCCCAGGACTTCACA...GGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGTGCGAGACTTGACGGCAGCGATAGGGACTTTTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 261 34 261 1 318 21 303 6 11 6 0 309 31 18 31 36 TGTGCGAGACTTGACGGCAGCGATAGGGACTTTTGG 324.0 0.8966 7e-91 4GA19AG8CA2AG20TACGGC3TG2GA46CA5TCCA5TA7AG1GA6CG10AG25GT6GC30TC1AGTCTC10AG7GC1AG1TG15 60.0 0.9706 2e-13 16AT17 ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCCAGACTTCT GTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGG ACCTATGCCCAGGACTTCACA...GGACGGTTTGTCTTCTCCGTGGACAGCTCTGTCAGCACGGCATATCTGCAGATCAGTAATTTAAAGGCTGAAGACACTGGCATTTATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACACCTTC............ACTTCGTATTCT ATTCACACCTAC......ACTGGAAGCCCA GCGAGACTTGACGGCAGCGATAGGGACTTT F,RC,F MID54 IgA-PCR LR3 4
SRR765688.42360 HQP9GKT01B8L6A length=364 NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCAAGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAGCATCCCCG T T F T IGHV2-5*02 IGHD1-20*01,IGHD1-7*01,IGHD1/OR15-1a*01 IGHJ4*02 CTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG ............................................................CTGACCTGCACCTTCTCTGGGTTCTCACTCACC......ACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGAT.........GATGATAAGCGCTACAGTCCATCTCTGAGG...AACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG 22 235 58 241 1 319 2 259 13 1 13 10 282 47 2 47 51 TGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGG 315.0 0.9253 4e-88 31CG5CG3CA20CT65TC10GA2AG15TC13-A-A-A-A-C-C10CT9GA8TC26AG6 77.8 0.9573999999999999 7e-19 25GA13GC7 ............................................................CTGACCTGCACCTTCTCT GTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTC CGCTACAGTCCATCTCTGAGG...AACAGGCTCACCATCACTAAGGACACCTCCA------AGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGT TGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAG GGGTTCTCACTCACC......ACTACTGGCGTGGGT ATTTATTGGGAT.........GATGATAAG ACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTAC F,F,F MID54 IgA-PCR LR8 2
SRR765688.38856 HQP9GKT01A7EJO length=362 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC F T T T IGHV6-1*01,IGHV6-1*02 IGHD2-15*01,IGHD3-3*01,IGHD3-3*02 IGHJ4*02 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATGATTATGCACCCTCTGTGGCAAGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......AGCAACAGTGCTACTTGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCC...CAGTGGTATAATGATTATGCACCCTCTGTGGCA...AGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 20 239 63 240 1 317 7 266 10 11 10 6 282 45 4 45 45 TGTGCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTACTGG 316.0 0.9292 1e-88 40AG5CA42-G19CA20CGCTCA6GACA13GA14GT3GA7GT11TC28ATCG1TG14 89.7 1.0 2e-22 45 .................................................................CTGTGCCATCTCC TGGCACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGG GATTATGCACCCTCTGTGGCA...AGTCGAATAACCGTCAACCCAGACACAGCCAGGAACCAGGTCTCCCTGCAGTTGAACTCTGTGACTCCCGAGGACACGGCACTTTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGGGACAGTGTCTCT......AGCAACAGTGCTACT ACATACTACAGGTCC...CAGTGGTATAAT GCAAGGGCTTGGAGTGGTGGTAATTACCACTTTGACTAC F,RC,F MID54 IgG-PCR LR1 2
SRR765688.23240 HQP9GKT01BYYSD length=378 NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ...........................................CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTC............ACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAACCCAAGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 255 41 255 1 319 4 280 7 11 7 15 302 40 9 40 45 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG 318.0 0.898 5e-89 17AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC13TA8AG11AG5TC10 71.9 0.975 5e-17 22AT17 ...........................................CTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCT ATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGG AGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACATGTTC............ACTTCGTATTCT ATCAACACCAAC......ACTGGGAACCCA GCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTAC F,RC,F MID53 IgA-PCR LR2 2
SRR765688.45110 HQP9GKT01BTIR2 length=360 NNNNNNNNNNNNNNNNNNNCTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD7-27*01 IGHJ6*02 CTGTGCCGTCTCCGGAGACAGTATCTCTAACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCCAAGTGGTCTACTGATTATGCAGAATCTGTGAAAAGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA .................................................................CTGTGCCGTCTCCGGAGACAGTATCTCT......AACAAGAATGCTGGTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACCTTCTACAGGTCC...AAGTGGTCTACTGATTATGCAGAATCTGTGAAA...AGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 20 242 63 242 1 319 8 270 7 5 7 2 279 45 18 45 42 TGTACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTCTGG 332.0 0.938 3e-93 7GA7AG6AG6AG3GC1AG5GC54CA1TA17CA2CA11AT48CG11AG42AG6 81.8 0.9778 5e-20 6AG38 .................................................................CTGTGCCGTCTCC TGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG GATTATGCAGAATCTGTGAAA...AGTCGAATAACCATCAACCCAGACACATCCAAGAACCACTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGAGACAGTATCTCT......AACAAGAATGCTGGT ACCTTCTACAGGTCC...AAGTGGTCTACT ACAAGAGTCGAACACCTGGGGAGGGGTATGAACGTC F,RC,F MID53 IgG-PCR LR1 2
SRR765688.41920 HQP9GKT01DGU7R length=383 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTC............ACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAACCCAAGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 262 34 262 1 319 4 287 7 11 7 15 309 40 9 40 45 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG 329.0 0.9007999999999999 3e-92 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14TC12GC13TA8AG11AG5TC10 71.9 0.975 5e-17 22AT17 ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCT ATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGG AGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACATGTTC............ACTTCGTATTCT ATCAACACCAAC......ACTGGGAACCCA GCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTAC F,RC,F MID53 IgA-PCR LR3 4
SRR765688.5099 HQP9GKT01AS88P length=374 NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD6-25*01 IGHJ4*02 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTC............ACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAA......ACTGGGAACCCAACCTATGCCCAGGACTTCACA...GGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 261 34 261 1 318 7 289 6 8 6 5 300 40 9 40 36 TGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGG 343.0 0.9195 2e-96 4GA19AG11AG20TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT46TC6AG4AG5CT3AG1TG15 63.9 0.95 1e-14 4TA17AT17 ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCT GTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGG ACCTATGCCCAGGACTTCACA...GGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACACCTTC............ACTTCCTATTCT ATTCACACCCAA......ACTGGGAACCCA GCGAGACTCGACGGCAGCGATAGGGACTTC F,RC,F MID54 IgG-PCR LR3 5
SRR765688.41370 HQP9GKT01DK3O2 length=377 NNNNNNNNNNNNNNNNNNNNAAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD3-10*01,IGHD3-10*02,IGHD3-3*01 IGHJ5*02 AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTC............AAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAAT......ACTAGAAGGCCAACATATGCCCCGGGCTTCGCA...GGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 263 34 263 1 320 5 289 6 24 6 5 300 43 9 43 39 TGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGG 296.0 0.8593000000000001 2e-82 3GA18CA1AG14ATTC2AG5AC5ACATTA1TC1GA1AGGC3AG6CG46TCGATC3TC3AG1AG1GAGC5AG7CA7GA7AG18GA14AG1TA16AG3CA3AG4AG11TG5TC11 85.7 1.0 3e-21 43 ....................................AAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATT ATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACATATGCCCCGGGCTTCGCA...GGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GAATACAACTTC............AAATGTTGTAGT ATCAATGTCAAT......ACTAGAAGGCCA GCGAGAGATCTAAATTATATACACTTCGACCCC F,RC,F MID54 IgG-PCR LR3 3
SRR765688.46467 HQP9GKT01EZF79 length=377 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG T T F F IGHV1-46*01 IGHD1-1*01,IGHD1-20*01,IGHD2-21*01 IGHJ6*02 AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA .......................................AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTC............ACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGT......GGTGGTAGCACAAGCTACGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCA 21 260 37 260 1 320 8 289 5 13 5 0 294 48 15 48 42 TGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGG 403.0 0.9962000000000001 8e-115 29TC230 91.7 0.98 5e-23 42CT7 .......................................AAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCT ATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATA AGCTACGCACAGAAGTTCCAG...GGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGT TGGGGCCAAGGGACCACGGTCACCGCCTCCTCA GGATACACCTTC............ACCAGCTACTAT ATCAACCCTAGT......GGTGGTAGCACA GCGAGAGATTCCTTGCACGACTACGGTATGGACGTC F,F,F MID54 IgA-PCR LR9 2
SRR765688.17960 HQP9GKT01CN8ZX length=360 NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAGCATCCCCG T T F F IGHV2-70D*04 IGHD2-21*02 IGHJ4*02 ACTGACCTGCACCTTCTCTGGCTTTTCACTCAGTAGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGATGATTATAAATTCTACAACACATCTCTGAGGACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG ...........................................................ACTGACCTGCACCTTCTCTGGCTTTTCACTCAGT......AGTCGTACAATGCGTGTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGCATTGATTGGGAT.........GATTATAAATTCTACAACACATCTCTGAGG...ACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG 21 243 57 243 1 320 15 279 7 17 7 0 286 43 6 43 45 TGTGCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTACTGG 321.0 0.9218000000000001 6e-90 21CG2TC8TC1GC1CA2AGCG10GA7TC9TC13AG30TG12AG11GA10TA23GA25TC10CT27CG2 69.9 0.9534999999999999 2e-16 22TC9TC10 ...........................................................ACTGACCTGCACCTTCTCT GTGGGCTGGATTCGTCAGCCCTCAGGGAAGGCCCTAGAGTGGCTTGCACGC TTCTACAACACATCTCTGAGG...ACCAGGCTCTCCATCTCCAAGGACACCTCCAAAGACCAGGTGGTCCTTACAATGACCAATATGGACCCTGCGGACACAGCCACGTATTACTGT TGGGGCCAGGGAATCCTGGTCACTGTCTCCTCAG GGCTTTTCACTCAGT......AGTCGTACAATGCGT ATTGATTGGGAT.........GATTATAAA GCACGCATCGCGGGAGGTGAGGGGACTGCTTTTGACTAC F,F,F MID54 IgA-PCR LR8 4
SRR765688.25497 HQP9GKT01B88YS length=389 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG F F T T IGHV4-39*01 IGHD1-7*01 IGHJ4*02 CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGC......AGTAGTAGTTGCTACTGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTAT---AGT.........GGGAGTACCTACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 246 50 250 1 320 14 281 8 10 8 12 301 47 2 47 61 TGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCTTGG 332.0 0.94 3e-93 17GA12AG20GA16-C33CG10-T-A-T8TC8TC29GA11CG23CG5GC26AG17 77.8 0.9573999999999999 8e-19 11CATC34 ....................................................CCCTGTCCCTCACCTGCGCTGTCTCT TGGGGCTGGATC-GCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGT TACTATAACCCGTCCCTCAAG...AGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGACTCCATCAGC......AGTAGTAGTTGCTAC ATCTAT---AGT.........GGGAGTACC GCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCTACTTTGACTCT F,RC,F MID54 IgA-PCR LR7 2
SRR765688.39627 HQP9GKT01CATH4 length=374 NNNNNNNNNNNNNNNNNNNNNCCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAGCCTCCAC T T F F IGHV4-59*08 IGHD2-15*01,IGHD3-22*01,IGHD3-3*01 IGHJ5*02 CCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATCATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGATGGAACCACGACCTACAACCCCTCCCTCAGGAGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG ....................................................CCCTGTCGCTCATCTGCACTGTCTCTGGTGGCTCCATC............ATTGATTACTACTGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTATATCTATTATGAT.........GGAACCACGACCTACAACCCCTCCCTCAGG...AGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG 22 244 50 244 1 320 5 271 6 11 6 11 288 46 6 46 48 TGTGTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCCTGG 266.0 0.8484 1e-73 7GC4TC26TG1GAAG9CG2TC8TG3AC4TA1CG1CA4CA14TG11TCGAAG3AG1CG3GC1CA17GA6CA1CT12TA15GC2AT8GA1CG4CG25AGCTAG5TC4TCTGCA4 83.8 0.9783 1e-20 36AG9 ....................................................CCCTGTCGCTCATCTGCACTGTCTCT TGCAGTTGGATCCGTCAGACCCCTGCGCAGGGCCTGGAGTGGATTGGTTAT ACCTACAACCCCTCCCTCAGG...AGTCGCGCCACCATATCAGTTGACACGTCCAAGAACGAGATCTCCCTGGACCTGACCTCTGTGACCGCCGCAGACACGGCCACATATTATTGT TGGGGCCAGGGAACCCTGGTCACCATCTCCTCAG GGTGGCTCCATC............ATTGATTACTAC ATCTATTATGAT.........GGAACCACG GTTCGACATGGAAAGTGGTCGGACCAGGGATGGTTCGACCCC F,RC,F MID53 IgG-PCR LR7 2
SRR765688.48095 HQP9GKT01DC6RH length=389 NNNNNNNNNNNNNNNNNNNCTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-31*03 IGHD3-10*01,IGHD3-10*02 IGHJ5*02 CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACTGGGAACACCTATTACAATCCGTCCCTCAAGAGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG ...........................................CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGC......AGTGATGGTTCTTACTGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATACATCTCTTACACT.........GGGAACACCTATTACAATCCGTCCCTCAAG...AGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGTGCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCCTGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG 20 258 41 258 1 319 16 294 15 13 15 2 311 45 7 45 57 TGTGCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCCTGG 350.0 0.9340999999999999 8e-99 54AG5CATC7AG33AG8AG7CA5CG5AG6TC5TC26TA23AG2TC8AGTC31CG16 81.8 0.9778 5e-20 23TA21 ...........................................CTTCACAGACCCTGTCCCTCACCTGCACTGTCTCT TGGAACTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAATGGATTGGATAC TATTACAATCCGTCCCTCAAG...AGTCGAGTTACCATTTCAGTAGACACGTCTAAGAACCAATTTTCCCTGAAATTGAGCTCTGTGACTGCCGCGGACACGGCCGTCTATTACTGT TGGGGCCAGGGATCCCTGGTCACCGTCTCCTCAG GGTGGCTCCATCAGC......AGTGATGGTTCTTAC ATCTCTTACACT.........GGGAACACC GCGAGAGCGAGAGATGACTATACTTCGGGGAGTTATTACGGGTTCGACCCC F,RC,F MID53 IgA-PCR LR4 2
SRR765688.32938 HQP9GKT01CBIO9 length=387 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD5-12*01,IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTCATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACAGCTTC............ATTAATTATGCTCTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAACCCAACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGTGCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 263 34 263 1 320 9 293 10 2 10 14 317 38 11 38 51 TGTGCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTACTGG 386.0 0.9695999999999999 1e-109 49GC5TC2AGTC6CA136CA13AG15CG29 75.8 1.0 3e-18 38 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCT CTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCCTATCTGCAGATCAACAGCCTAAAGGCTGACGACACTGCCGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACAGCTTC............ATTAATTATGCT ATCAACACCAAC......ACTGGGAACCCA GCGAGAGATCGGGGGCATGGATATAGTTATGGTTATTCTGGCTAC F,F,F MID54 IgA-PCR LR3 2
SRR765688.33355 HQP9GKT01B9Y0F length=369 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV7-4-1*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02,IGHJ5*02 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTTTCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCACTGGGAACCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCTGGATACACATTT............TCTACCCATGTTATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATC......ACTGGGAACCCAACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGGATTCGACACCGGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 263 34 262 1 319 0 284 3 3 3 13 300 35 14 35 30 TGTGCGAGAGGATTCGACACCGGCCTCTGG 361.0 0.9468000000000001 4e-102 38CT11AC2TCTA3CG1CT3TC3AG20C-19GA18TA64AG3CG27GA37 69.9 1.0 2e-16 35 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCCTCT ATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGG ACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTATCACCACGGCATATCTGCAGATCAGCAGCCTGAAGGCTGAGGACACTGCCGTGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACATTT............TCTACCCATGTT ATCAACACCATC......ACTGGGAACCCA GCGAGAGGATTCGACACCGGCCTC F,RC,F MID53 IgA-PCR LR3 2
SRR765688.32244 HQP9GKT01C73BZ length=376 NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCATCCCCG T T F F IGHV1-69*06,IGHV1-69*14 IGHD1-7*01 IGHJ2*01 CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTC............AGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCC......TTTGGAACATTCCACTACGCACAGAAATTTCAG...GGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG 21 256 41 256 1 320 5 282 7 10 7 5 294 48 6 48 45 TGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGG 300.0 0.875 7e-84 27GA11AG2TCTC5AC1CG5GC5CGTC26CG27GACT6AT3TGTCCACA13AG2TC9CG4CG14CA4TC1GACGTC4TC16GA6CG18AG5TC6CA4 87.7 0.9792000000000001 8e-22 6CT41 ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCT ATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGG CACTACGCACAGAAATTTCAG...GGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGT TGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG GGAGACATTTTC............AGAACCTATGGT ATCATCCCTGCC......TTTGGAACATTC GCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTC F,RC,F MID54 IgA-PCR LR2 2
SRR765688.17491 HQP9GKT01BUYPI length=378 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV5-10-1*01,IGHV5-10-1*03 IGHD6-13*01 IGHJ4*02 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTT............ACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGT......GACTCTTATACCAACTACAGCCCGTCCTTCCAA...GGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 254 41 254 1 318 4 279 17 5 17 7 303 42 7 42 48 TGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGG 397.0 1.0 6e-113 254 83.8 1.0 1e-20 42 ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCT ATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGG AACTACAGCCCGTCCTTCCAA...GGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACAGCTTT............ACCAGCTACTGG ATTGATCCTAGT......GACTCTTATACC GCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTAC F,F,F MID54 IgG-PCR LR2,LR5 2
SRR765688.13678 HQP9GKT01BUUUG length=369 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F T IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATC............AGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATT.........GAGACCACCTATTACAA-CCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 242 50 244 1 320 14 277 7 9 7 13 297 37 15 37 51 TGTGCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCCTGG 294.0 0.9016 5e-82 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14NC5-C7GA5AGTC3CG25CT1TA12 73.8 1.0 1e-17 37 ....................................................CCCTGTCCCTCACCTGCACTGTCTCT TGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATAT TATTACAA-CCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAANCAGTT-TCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGGCTCCATC............AGAGGATACTAT ATCTATGACATT.........GAGACCACC GCGAGACAGTTCCGGGCCTAACAGTGTCTTGTTCTGGTCCGACCC F,RC,F MID53 IgA-PCR LR7 2
SRR765688.7787 HQP9GKT01COO0Q length=360 NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCCTCCAC T T F T IGHV2-5*02 IGHD3-16*01,IGHD3-16*02,IGHD5-24*01 IGHJ4*02 GCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG ...........................................................GCTGACCTGCACCTTCTCTGGATTCTCACTCAGT......ACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGAT.........GATGATTACAGCACCAGCCCATCTCTGAAG...AGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG 21 249 57 243 1 320 15 285 7 7 7 0 292 34 15 34 36 TGTGCACACAGTGCGGGATGGCTGCCTGATTACTGG 318.0 0.9157 5e-89 21AG11TC7CG9AGCGAG62C-C-A-A-A-T-2TA1CGAC2ATCA50GA7AG4CT22CT30 65.9 0.973 3e-15 32TC4 ...........................................................GCTGACCTGCACCTTCTCT GTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTC AGCACCAGCCCATCTCTGAAG...AGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAG GGATTCTCACTCAGT......ACTAGTGCAGTGGGT ATTTATTGGGAT.........GATGATTAC GCACACAGTGCGGGATGGCTGCCTGATTAC F,RC,F MID53 IgG-PCR LR8 3
SRR765688.43055 HQP9GKT01ANQEB length=386 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC F T T T IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTT............GACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAA......GACTCTGATACCAGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC 21 254 41 253 1 317 27 302 7 22 7 9 318 32 15 32 54 TGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGG 288.0 0.8701000000000001 4e-80 16AG1A-13GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3 56.0 0.9688 3e-12 16AT15 ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCT CTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTC AGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC GGATACAACTTT............GACACCTATTGG ATCTATCCTGAA......GACTCTGATACC GTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTAT F,F,F MID54 IgG-PCR LR2,LR5 3
SRR765688.24232 HQP9GKT01BTWAC length=385 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC T T F F IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTT............GACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAA......GACTCTGATACCAGATATACTTCGTCCTTCCAC...GGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC 21 253 41 253 1 317 27 301 7 22 7 6 314 35 12 35 54 TGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGG 293.0 0.8695999999999999 2e-81 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT17TC1CGTCTC10CA5CG8AC10GA9TC4GC7GC7TC7GA1AGTC8GC6AG10TC3 61.9 0.9714 5e-14 19AT15 ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCT CTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTC AGATATACTTCGTCCTTCCAC...GGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC GGATACAACTTT............GACACCTATTGG ATCTATCCTGAA......GACTCTGATACC GTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTAC F,RC,F MID54 IgG-PCR LR2 2
SRR765688.35786 HQP9GKT01DW0J7 length=382 NNNNNNNNNNNNNNNNNNNNCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV1-18*04 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ...........................................CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTT............ACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTAC......AATGGTGACACAAAGTATGCACAGAAATTCCAG...GGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 256 41 256 1 320 5 282 10 12 10 14 306 42 7 42 51 TGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGG 366.0 0.9570000000000001 1e-103 27CA24TC52CG22GA7GC11AGTC32CT36TG18AG5TC11 83.8 1.0 1e-20 42 ...........................................CTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCT ATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGG AAGTATGCACAGAAATTCCAG...GGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTTACACCTTT............ACCAGTTACGGT ATCAGCGCTTAC......AATGGTGACACA GCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTAC F,F,F MID54 IgA-PCR LR2 2
SRR765688.45263 HQP9GKT01CZ91S length=353 NNNNNNNNNNNNNNNNNNNCTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAGCATCCCCG T T F F IGHV4-31*03 IGHD2/OR15-2a*01,IGHD2/OR15-2b*01 IGHJ3*02 CTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAG .................................................................CTGCTCTGTCTCTGGTGGCTCCATCACT......AGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGT.........GGTACCACCAACTACAACCCGGCCCTCAAG...AGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAG 20 235 63 235 1 318 0 255 6 4 6 8 269 50 1 50 42 TGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGG 280.0 0.8809 9e-78 4TA21CGTC18TACG4GA16AG20TG2TCGA3TA9TG1CG4AT11GT13CA2CTTA4TA5TA28CACG4CG4CG1AG18AG1TG9AG5 83.8 0.96 1e-20 15GC8CA25 .................................................................CTGCTCTGTCTCT TGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTAT AACTACAACCCGGCCCTCAAG...AGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGT TGGGGCCACGGGACAATGGTCACCGTCTCTTCAG GGTGGCTCCATCACT......AGTGGTGGTTACTAC GTCTTTTACAGT.........GGTACCACC ACGAGAATATTGGGGAGAGATGATGCTTTTGATATG F,F,F MID54 IgA-PCR LR1 2
SRR765688.48840 HQP9GKT01BLP5E length=367 NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAGCATCCCCG T T F F IGHV2-5*02 IGHD2-21*02,IGHD2-8*02,IGHD3-10*01 IGHJ3*01,IGHJ3*02 GCTGACCTGCACCGTCTCTGGCTTCTCACTCGCCACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGATAATGAGAAGCGGTACAGCCCACCTCTTAAGACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAG ...........................................................GCTGACCTGCACCGTCTCTGGCTTCTCACTCGCC......ACTGGTGGAATTGCTGTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCATATATATTGGGAT.........AATGAGAAGCGGTACAGCCCACCTCTTAAG...ACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAG 21 242 57 242 1 319 19 282 7 17 7 0 289 43 8 43 48 TGTGCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTCTGG 272.0 0.8595 2e-75 13GT7CG9GACG4GA5AG1TG1CG10TA28AG7TCTA1ATTC2AT9AG4GT5GC9CT4TG4CG3AG6GA2GA16CA1GA12AT2GA3GA14TG1AG17TC5GA1GA2 54.0 0.8936 1e-11 10CG17GAAT4CAAC11 ...........................................................GCTGACCTGCACCGTCTCT GTGGGCTGGTTCCGTCAGCCCCCAGGAAAGGCCCTGGAATGGCTTGTTCAT CGGTACAGCCCACCTCTTAAG...ACCAGACTCACCGTCGCCAAGGACACCTCCAACAGCCAGGTGGTCCTAACGATGGCCAACATGGACCCTTTAGACACAGCCACATATTATTGT TGGGGCCAAGGGACAGAGGTCCACGTCTCTTCAG GGCTTCTCACTCGCC......ACTGGTGGAATTGCT ATATATTGGGAT.........AATGAGAAG GCGCGCATGTATACTGGTTCGACGGGGACTGCTTTTGATCTC F,RC,F MID54 IgA-PCR LR8 2
SRR765688.36695 HQP9GKT01BVA49 length=383 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*02 IGHD6-19*01 IGHJ4*02 AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTC............ACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAACCCAAGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 262 34 262 1 319 4 287 7 11 7 15 309 40 9 40 45 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG 329.0 0.9007999999999999 3e-92 24AG11AG12TCGC6TACGGC3TG7CT13TC2GCCT52GCTG2CT1TC2CA3AC24GT1CG14GC12GC13TA8AG11AG5TC10 71.9 0.975 5e-17 22AT17 ....................................AAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCT ATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGG AGTTACGTCCCGGGATTCACA...GGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACATGTTC............ACTTCGTATTCT ATCAACACCAAC......ACTGGGAACCCA GCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTAC F,RC,F MID53 IgA-PCR LR3 5
SRR765688.13959 HQP9GKT01AER44 length=371 NNNNNNNNNNNNNNNNNNNCTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAGTGGCTACAGATATACCCCAGGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV3-11*06 IGHD5-12*01 IGHJ6*02 CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTCAGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACCAGTACTTACACAAACTACGCAGCCTCTGTGAAGGGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAGTGGCTACAGATATACCCCAGGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCA ...........................................CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCTGGATTCAGCCTC............AGTGACTACTATATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTACTACC......AGTACTTACACAAACTACGCAGCCTCTGTGAAG...GGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGTGCGAGAGTGGCTACAGATATACCCCAGGACGTCTGGGGCCAAGGGGCCACGGTCACCGTCTCCTCA 20 255 41 255 1 319 0 275 7 11 7 12 294 40 23 40 39 TGTGCGAGAGTGGCTACAGATATACCCCAGGACGTCTGG 364.0 0.9569 4e-103 13AG28GC1CT13TC58CG2CGCT4CG17CA19GA73AG16 71.9 0.975 5e-17 19GA20 ...........................................CTGGAGGGTCCCTAAGACTCTCCTGTGCAGCCTCT ATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATAC AACTACGCAGCCTCTGTGAAG...GGCCGATTCGCCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATATTACTGT TGGGGCCAAGGGGCCACGGTCACCGTCTCCTCA GGATTCAGCCTC............AGTGACTACTAT ATTAGTACTACC......AGTACTTACACA GCGAGAGTGGCTACAGATATACCCCAGGACGTC F,RC,F MID53 IgG-PCR LR4 2
SRR765688.24180 HQP9GKT01CPLH4 length=353 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGATTACAATTATGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD2-8*01,IGHD2-8*02,IGHD5-24*01 IGHJ6*02 CTGTGCCATCTCCGGCGACACTATCTCTAATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGGGTAGTGATTATGCAGTCTCTGTGAAAAGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGATTACAATTATGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA .................................................................CTGTGCCATCTCCGGCGACACTATCTCT......AATGACAGAACTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCC...AAGTGGGGTAGTGATTATGCAGTCTCTGTGAAA...AGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGTGCAAGAGATTACAATTATGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 20 243 63 243 1 320 7 270 8 21 8 0 278 40 23 40 36 TGTGCAAGAGATTACAATTATGCTATGGACGTCTGG 318.0 0.9177 5e-89 15CG4CG1AG6AGTCGA4ATAG10TC66GTGA2GA12CA17GA2TC6GC32TC4TC2AT17TG23 85.7 1.0 3e-21 43 .................................................................CTGTGCCATCTCC TGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG GATTATGCAGTCTCTGTGAAA...AGTCGAATGACTATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAGTTGAATTCAGTGACTCCCGAGGACACTGCTGTGTATTACTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGCGACACTATCTCT......AATGACAGAACTGCT ACATACTACAGGTCC...AAGTGGGGTAGT GCAAGAGATTACAATTATGCTATGGACGTC F,F,F MID53 IgG-PCR LR1 3
SRR765688.43187 HQP9GKT01C588C length=382 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGGGGC T T F F IGHV6-1*01,IGHV6-1*02 IGHD3-22*01 IGHJ6*02,IGHJ6*04 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAGTTTCTGTGAAGAGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGGGGC .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......AGCAACATTGCTGCTTGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCC...AAGTGGTATAATGATTATGCAGTTTCTGTGAAG...AGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGGGGC 20 243 63 243 1 320 10 273 21 2 21 9 303 30 6 30 81 TGTGCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGGGGC 358.0 0.9712000000000001 3e-101 35TG11GA84TA8GA9GA61CG24CA4 60.0 1.0 2e-13 30 .................................................................CTGTGCCATCTCC TGGAGCTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG GATTATGCAGTTTCTGTGAAG...AGTCGAATAGCCATCAACCCAGACACATCCAAGAACCAGTTCTCCCTGCAGCTGAACTCTGTGACTCCCGACGACACGGCTGTGTATTACTGT GGC GGGGACAGTGTCTCT......AGCAACATTGCTGCT ACATACTACAGGTCC...AAGTGGTATAAT GCACGAGATCCCGCCGACTATTACTATGATAATAGTGGTCACCTAATTTACTACTACTACGGTATGGACGTCTGG F,F,F MID54 IgG-PCR LR1 3
SRR765688.46667 HQP9GKT01COT4Z length=380 NNNNNNNNNNNNNNNNNNCCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV4-31*03 IGHD4-17*01,IGHD4-23*01 IGHJ1*01 CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG ..........................................CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACC......AGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGC.........GGGAGCGCCTGGTACAACCCGTCCCTGAAG...AGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 19 256 40 256 1 316 1 276 7 4 7 13 296 51 2 51 48 TGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGG 329.0 0.9102 3e-92 28GC16CA3CG5TG2AG16CA40TC11CT6GA3GAGC14GC9AG2TA2TA7CA17AG10TAAGGC3AG27CG5TC7 93.7 0.9804 1e-23 14TC36 ..........................................CCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCT TGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTAT TGGTACAACCCGTCCCTGAAG...AGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGT TGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG GGTGGCTCCCTCACC......AGTGTTGATTACTAC ATCTATTACAGC.........GGGAGCGCC GCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTAC F,RC,F MID54 IgG-PCR LR10 3
SRR765688.38963 HQP9GKT01C40UI length=376 NNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV2-70*11 IGHD3-3*01 IGHJ4*02 ACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ...........................................................ACTGACCTGCACCTTCTCTGGATTCTCACTCAGT......AGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGAT.........GATGATAAATACTACCGTACATCTCTGAAG...ACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 244 57 244 1 321 7 272 16 6 16 5 293 48 1 48 57 TGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGG 329.0 0.9303 3e-92 21AG11TC1GC10AT5GA74CA1TC14GC2AG20TC1TC2GA15CT29CA8CT1TA10TA2 87.7 0.9792000000000001 8e-22 11CT36 ...........................................................ACTGACCTGCACCTTCTCT GTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGC TACTACCGTACATCTCTGAAG...ACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATTCTCACTCAGT......AGTAGTGGAATGAGT ATTGATTGGGAT.........GATGATAAA GCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCAC F,RC,F MID53 IgG-PCR LR8 2
SRR765688.36155 HQP9GKT01BS5TB length=362 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV6-1*01,IGHV6-1*02 IGHD6-6*01 IGHJ4*02 CTGTGCCATCTCCGAGGACAGTGTCTCTGGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCCAAGTGGTATTATGAATATGCACCCTCTGTGAAAGGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .................................................................CTGTGCCATCTCCGAGGACAGTGTCTCT......GGCAACAGTGGTGCTTGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGGATTTTCTACAGGTCC...AAGTGGTATTATGAATATGCACCCTCTGTGAAA...GGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 20 242 63 242 1 319 5 267 14 4 14 2 283 45 4 45 45 TGTGCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTCTGG 294.0 0.8884000000000001 5e-82 14AG13GA9GC9TC32AG13TCTA1TA19TA4AT6CGCTCA9GA8GATC6TC14AG5AG11AG4CAGC2CT1CT9CG11TG5TC10 81.8 0.9778 5e-20 9TA35 .................................................................CTGTGCCATCTCC TGGAATTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAATGGCTGGGAAGG GAATATGCACCCTCTGTGAAA...GGTCGAATAGTCATCAATCCAGACACATCCAAAAACCAATTCTCCCTGCAACTGACGTCCGCGACTCCCGACGACACGGCTGTTTATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GAGGACAGTGTCTCT......GGCAACAGTGGTGCT ATTTTCTACAGGTCC...AAGTGGTATTAT GCAAGAGCGAAATATAGCAGCTCGTCAAACTTTGACTTC F,F,F MID53 IgA-PCR LR1 3
SRR765688.18975 HQP9GKT01BH3GZ length=377 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*01 IGHD3-3*01,IGHD3-3*02 IGHJ4*02 CCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATC............AGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGT.........GGGCGTACCGCTTACAGCCCCTCCCTCAAG...AGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG 21 243 50 243 1 319 13 277 14 13 14 9 300 42 7 42 57 TGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGG 318.0 0.9177 5e-89 17TA1CTAG20CACG18TC6TC8CA10AG27CA1TC3GACATC4GA27GA3CG19AG11CG3GA45 75.8 0.9762000000000001 3e-18 19CA22 ....................................................CCCTGTCCCTCACCTGCTCCATCTCT TGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTAT GCTTACAGCCCCTCCCTCAAG...AGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGT TGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG GGTGGCTCCATC............AGTCCTTACTAC ATCTATTACAGT.........GGGCGTACC GCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTAC F,RC,F MID53 IgA-PCR LR7 2
SRR765688.46037 HQP9GKT01APHCO length=359 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAGCCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD6-19*01 IGHJ5*02 CTGTGCCATCTCCGGAGTCGATGTCTCTACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCCCAGTGGATTAATGATTATGCAGTGTCTGTGAAGAGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG .................................................................CTGTGCCATCTCCGGAGTCGATGTCTCT......ACCTACAGTGCTTCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGGACTTATTACAATTCC...CAGTGGATTAATGATTATGCAGTGTCTGTGAAG...AGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG 20 242 63 242 1 319 6 268 14 8 14 7 289 36 16 36 42 TGTGTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACCTGG 300.0 0.8967 7e-84 15AG1TA1GAAG8CG1TA8TG11GA37CA5TA2TC4AGTG3CA5ATTA15GA8GA32AG6GT10CG5TC14CG3GA18TC5 56.0 0.9444 3e-12 19AG8GC7 .................................................................CTGTGCCATCTCC TGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGCAGG GATTATGCAGTGTCTGTGAAG...AGTCGAATAACCATCAACCCAGACACATCCAAAAACCAGGTCTCCCTGCACCTGAATTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGT TGGGGCCAGGGAACCCTAGTCACCGTGTCCTCAG GGAGTCGATGTCTCT......ACCTACAGTGCTTCT ACTTATTACAATTCC...CAGTGGATTAAT GTAAGAGGTTATGGCAGTGGCTGGTACTGGATAACC F,F,F MID54 IgG-PCR LR1 3
SRR765688.15636 HQP9GKT01ATB6I length=377 NNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCTGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV2-5*02 IGHD2-21*02 IGHJ4*02 GCTGACCTGCACCTTCTCTGGGTTCTCAATCGAAGCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGATGATGATAGATACTACACCTCATCTCTCAAGAGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG ...........................................................GCTGACCTGCACCTTCTCTGGGTTCTCAATCGAA......GCTGATGGAGTGGCTGTGGGCTGGATCCGTCAGCCCCCCGGAGAGGCCCTGGAGTGGCTTGCACTCTACTTTTGGGAT.........GATGATAGATACTACACCTCATCTCTCAAG...AGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG 21 244 57 243 1 320 9 274 8 13 8 15 297 48 1 48 60 TGTGTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTACTGG 269.0 0.8606999999999999 2e-74 28AC2GAAGACGA2GAAG8CG24CAT-3GA23TAATCT1TA14GAAGTCAG5CG1TC7CG5GC8TC5TC13GA6CG3AG6GCGA19CT23TCCA1TA3 87.7 0.9792000000000001 8e-22 26GA21 ...........................................................GCTGACCTGCACCTTCTCT GTGGGCTGGATCCGTCAGCCCCCCGGAGAGGCCCTGGAGTGGCTTGCACTC TACTACACCTCATCTCTCAAG...AGGAGGCTCACTATCACTAAGGACACCTCCAGAAACCACGTGATCCTTAGGATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGT TGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG GGGTTCTCAATCGAA......GCTGATGGAGTGGCT TACTTTTGGGAT.........GATGATAGA GTCCTCAGTCGCCGTCTTGGTGACTCCGGAGTGCAGAAATACTACTTTGACTAC F,RC,F MID54 IgA-PCR LR8 2
SRR765688.40959 HQP9GKT01BMXS4 length=368 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*08 IGHD3-16*01,IGHD3-16*02,IGHD5-24*01 IGHJ4*02 CCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATCAGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGTGGAAGCACCAACTACAATCCCTCCCTCAGGAGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCTCTGTCTCTGGTGGCTCCATC............AGTGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACATGTCCATTCTGGT.........GGAAGCACCAACTACAATCCCTCCCTCAGG...AGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAG 21 242 50 242 1 318 0 263 3 24 3 14 280 47 2 47 42 TGCGCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTATTGC 285.0 0.8759999999999999 3e-79 17TA23GA12CG13TC13CATC13AGCT2GA2CT3CATCGA4AG14TC10GA10GAAC1TA7CA17CG8AG1GAAGAC3CG3CT23CG5TC2CT6 54.0 0.8936 1e-11 12TC2CG10TCTC1AT17 ....................................................CCCTGTCCCTCACCTGCTCTGTCTCT TGGACCTGGATCCGGCAGTCCCCAGGGAAGGGCTTGGAGTGGATTGGACAT AACTACAATCCCTCCCTCAGG...AGTCGAGTCGACTTATCAGTCGACACGTCCAAGAACCACTTCTCCCTAAGAATGACCTCCGTGACCGCCGCAGACACGGCCGTCTATTATTGC TGCGGCCAGGGAATTCAGGTCACCGTCTCCTCAG GGTGGCTCCATC............AGTGGTTACTAC GTCCATTCTGGT.........GGAAGCACC GCGAGAGTTCAATTTCCTAGGTTCTACTTTGACTAT F,RC,F MID53 IgA-PCR LR7 2
SRR765688.44149 HQP9GKT01CLVEF length=360 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC T T F F IGHV7-4-1*02 IGHD1-26*01 IGHJ6*01 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTC............ACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAAC......ACTGGGACCCCAACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC 21 263 34 263 1 320 0 284 7 5 7 0 291 19 18 19 37 TGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC 392.0 0.9772 1e-111 63TG5CA49TC3TATC11CA126 38.2 1.0 6e-07 19 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCT ATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACGTATGCCCAGGGCTTCACA...GGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGT GGC GGATACACCTTC............ACTAGCTATTCT ATTAACTTCAAC......ACTGGGACCCCA GCGAGAGATAGTGGGGGTATGGACGTCTGGG F,F,F MID54 IgG-PCR LR3 2
SRR765688.9210 HQP9GKT01ED1VA length=368 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAGCCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD1/OR15-1a*01,IGHD1/OR15-1b*01,IGHD6-19*01 IGHJ4*02,IGHJ5*02 CTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......CGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCC...AGGTGGTTTTATGAGTATTCAGTGTCTCTACAA...GGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG 20 242 63 242 1 319 5 267 6 11 6 27 300 34 15 34 51 TGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGG 266.0 0.8512000000000001 1e-73 28CA3GA2TG2TC1AG1CT17AG5GA28TCCA13GA5TA1TA4GT3TG4GA3CG1AGCA2GA7GACAGC1TA2TA3AC18AGCT10AGTC4TC1TC5GC6AG11CG4TA11 44.1 0.9118 1e-08 6AC6TC9TC10 .................................................................CTGTGCCATCTCC TGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGG GAGTATTCAGTGTCTCTACAA...GGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGT TGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAG GGGGACAGTGTCTCT......CGCAGCATTGTTACC ATCTACTACAGGTCC...AGGTGGTTTTAT GCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAG F,RC,F MID53 IgG-PCR LR1 4
SRR765688.31439 HQP9GKT01BFFKN length=371 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATC............AGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATT.........GAGACCACCTATTACAACCCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 244 50 244 1 320 13 278 7 9 7 4 289 47 5 47 51 TGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGG 310.0 0.9057 1e-86 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC37CTGA14TC5TC7GA5AGTC3CG25CT1TA12 93.7 1.0 1e-23 47 ....................................................CCCTGTCCCTCACCTGCACTGTCTCT TGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATAT TATTACAACCCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGGCTCCATC............AGAGGATACTAT ATCTATGACATT.........GAGACCACC GCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCC F,RC,F MID53 IgA-PCR LR7 4
SRR765688.33456 HQP9GKT01BSJ79 length=384 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV1-18*04 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG .......................................AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTT............ACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTAC......AATGGTGACACAAAGTATGCACAGAAATTCCAG...GGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 260 37 260 1 320 5 286 10 12 10 14 310 42 7 42 51 TGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGG 372.0 0.9577 2e-105 31CA24TC52CG22GA7GC11AGTC32CT36TG18AG5TC11 83.8 1.0 1e-20 42 .......................................AAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCT ATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGG AAGTATGCACAGAAATTCCAG...GGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTTACACCTTT............ACCAGTTACGGT ATCAGCGCTTAC......AATGGTGACACA GCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTAC F,F,F MID54 IgA-PCR LR9 2
SRR765688.40824 HQP9GKT01B3H0W length=380 NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAGCCTCCAC T T F F IGHV1-69*06,IGHV1-69*14 IGHD1-26*01 IGHJ3*02 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTCCACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCCTTTGGTACAGCAGACTACGCACAGAAGCTCCAGGGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAG ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCTGGAGGCACCCTC............CACAAATATGGTCTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGGATCAGCCCTCCC......TTTGGTACAGCAGACTACGCACAGAAGCTCCAG...GGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGTGCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATGTGGGGCCAAGGGACACTGATCACCGTCTCTTCAG 21 255 41 255 1 319 4 280 13 8 13 9 302 46 5 46 51 TGTGCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATGTGG 308.0 0.8863 3e-86 29AG2CT11CT2CAAG2AGAC4GC1CA3CG13AGTC6CA5AG22GT4CACT13GA14CT16CG2GT3AG1AG4CA24AG13CG2TC13AG7TC10 67.9 0.9348000000000001 7e-16 11GC15CA2AG15 ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAAGACTCCT CTCACCTGGGTGCGACAGATCCCTGGCCAAGGACTTGAGTGGATGGGAGGG GACTACGCACAGAAGCTCCAG...GGCAGAGTCACCATGACCACAGACACATCCACGAGCACAGCCTACATGGAACTGAGCAGCCTGACATTTGAGGACACGGCCATGTATTATTGT TGGGGCCAAGGGACACTGATCACCGTCTCTTCAG GGAGGCACCCTC............CACAAATATGGT ATCAGCCCTCCC......TTTGGTACAGCA GCGAGAGGCATTGGGAGCTACTACCGTGATGACGCTTTTGATATG F,F,F MID54 IgG-PCR LR2 2
SRR765688.25750 HQP9GKT01CM4A4 length=362 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGACCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC F T T T IGHV4-34*01,IGHV4-34*02 IGHD6-19*01 IGHJ4*02 CCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCTAGTGGGTCCTACTGGAGCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGTGGAAGCCCCAACTACAACCCGTCCCTCAAGACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCGCTGTCCATGGTGGGTCCTCT............AGTGGGTCCTACTGGAGCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGGAAATGAAGGATAGT.........GGAAGCCCCAACTACAACCCGTCCCTCAAG...ACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 244 50 243 1 319 8 273 12 7 12 7 292 36 13 36 42 TGTGTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCACTGG 300.0 0.9016 7e-84 23CT12CTTC5GT1CA18TC3A-14AG20GC2GTGC11CA24CG4CG11AG18TATG11AG5GC5CG2TC13GC3TG10TC5 71.9 1.0 4e-17 36 ....................................................CCCTGTCCCTCACCTGCGCTGTCCAT TGGAGCTGGATCCGTCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGGAA AACTACAACCCGTCCCTCAAG...ACTCGACTCACCATATCAATAGACACGTCCAAGAACCTTTTCTCCCTGAAACTGAGGTCTGTCACTGCCGCGGACACGGGTGTTTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGGGTCCTCT............AGTGGGTCCTAC ATGAAGGATAGT.........GGAAGCCCC GTGAGAGATCCTTACAGCAGTGGCTGGCACGCCCAC F,RC,F MID53 IgG-PCR LR7 2
SRR765688.49935 HQP9GKT01BCQ7U length=391 NNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGAAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCAACCCTTTCCCCCT F T T T IGHV6-1*01,IGHV6-1*02 IGHD7-27*01 IGHJ4*02 CTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCCCAGTGGTATAATACTTATGCAGTCTCTGTGAAAGGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG .................................................................CTGTGCCATCTCCGGGGACAGTGTCTCT......AGCAACAGTGCTGCTTGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGGACATACTACAGGTCC...CAGTGGTATAATACTTATGCAGTCTCTGTGAAA...GGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG 20 240 63 241 1 318 10 270 11 1 11 8 289 44 5 44 51 TGTGCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCACTGG 343.0 0.9627 2e-96 52GA36-G19CA11AGCA9CA9GA14GA34TG48 71.9 0.9545 5e-17 7CT15AC20 .................................................................CTGTGCCATCTCC TGGAACTGGGTCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGG-AAGG ACTTATGCAGTCTCTGTGAAA...GGTCGAATAACCATCGACCCAGACACATCCAAGAACCAGTTCTCCCTGCATCTGAACTCTGTGACTCCCGAGGACACGGCTGTGTATTACTGT TGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG GGGGACAGTGTCTCT......AGCAACAGTGCTGCT ACATACTACAGGTCC...CAGTGGTATAAT GCAAGAAGTAAGAGGTCTAACTGGGGATCAGACTTCTTTGACCAC F,RC,F MID54 IgM-PCR LR1 2
SRR765688.46955 HQP9GKT01BB4XL length=379 NNNNNNNNNNNNNNNNNNNNACGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCCTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC F T T T IGHV7-4-1*02 IGHD6-13*01 IGHJ4*02,IGHJ5*02 GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTCACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATCAGTGGAAACCCAACCTACGCCCAGGCCTTCACATCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACACCTTC............ACTAAATATCCAATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGGATCAACACCATC......AGTGGAAACCCAACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 23 256 36 257 1 316 12 291 11 3 11 8 310 35 14 35 42 TGTTCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTCTGG 330.0 0.9183 9e-93 22AG33AGAC3CG1AT42GA18TA2GC3AG8CG2CT7CG7TGCG12TC3-T38GA8AG14AG1AG9TG3 69.9 1.0 2e-16 35 ......................................GAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGGTGGGATGG ACCTACGCCCAGGCCTTCACA...TCACGGTTTGTCTTTTCC-TGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCGGCCTAAAGACTGAGGACACTGCCATATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACCTTC............ACTAAATATCCA ATCAACACCATC......AGTGGAAACCCA TCGACCCTCCAACCGCGTATAGCAGCACCTGAGTTC F,F,F MID54 IgG-PCR LR3 2
SRR765688.41568 HQP9GKT01AV8YG length=399 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCACCATCCCCG T T F T IGHV1-69*01,IGHV1-69*04,IGHV1-69*09 IGHD1-26*01,IGHD2-2*02,IGHD2-21*01 IGHJ4*02 AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTCAGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACTGTTGGTTTAGCACACTTCGCACAGAACTTCCAGGGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA .......................................AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCTGCAGGCACTTTC............AGTTACCATGTTATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGGTTCATCCCAACT......GTTGGTTTAGCACACTTCGCACAGAACTTCCAG...GGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGTGCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA 21 260 37 257 1 317 14 295 7 7 7 18 320 43 5 43 60 TGTGCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGACTGG 269.0 0.8384999999999999 2e-74 30CA9CG6TC5TCTAAG1CT3TC3TCCAAG4CG4GA33CG2TA6C-A-A-1AT1CTTCGT5TATC4CA3TA9CG15GA1CG3TA10GA2TCGA3CG3CA6CA4AGTC19AG9AG1CG2CT11 77.8 0.9767 8e-19 7GT35 .......................................AAGCCTGGGTCCTCGGTGAAGGTCTCCTGCCAGGCTTCT ATTCACTGGCTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGACGG CACTTCGCACAGAACTTCCAG...GGCAGAGTCGCCATTTCCGCGGACGAGTCTGCGACCACCGCCTACCTGGAATTGAGCAGCCTGAGATCTGAAGACACGGCCATCTACTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA GCAGGCACTTTC............AGTTACCATGTT TTCATCCCAACT......GTTGGTTTAGCA GCGAGGTCGTCTTGTCCTGGTGGGAGTTGCTATATGTCCGCCTCCTTTGACGAC F,RC,F MID54 IgA-PCR LR9 2
SRR765688.24124 HQP9GKT01CMXTP length=364 NNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV4-59*01,IGHV4-59*02,IGHV4-59*07 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG .................................................................CTGCAGTGTCTCTGGCGGCGGCTCC............GGCCACTATTATTGGACTTGGATCCGGCAGCCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGG.........GCCAATTTCAAGTACAACCCCTCCGTGAAG...AATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG 20 229 63 228 1 317 1 250 7 2 7 29 286 43 6 43 57 TGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGG 199.0 0.7859999999999999 2e-53 5GC9CT3GTGC1TACT1GA1CTCAAGCT2TC2TC4CGTC12T-12AGCG6AG3CA4CG1TACT6GT1TCGA1GT1CGCG1AGTCTATC3GC12GC1GC4AG12GA9CA8GAGA4AT1GC9GC3CG8GCTC1TC15CG14 61.9 0.9301999999999999 4e-14 8TC13TC2GT17 .................................................................CTGCAGTGTCTCT TGGACTTGGATCCGGCAGCCCCCAGGGAAGACACTGGAATGGCTTGGCTTC AAGTACAACCCCTCCGTGAAG...AATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGT TGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAG GGCGGCGGCTCC............GGCCACTATTAT ATCTATGATGGG.........GCCAATTTC GCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTAT F,RC,F MID54 IgA-PCR LR1 8
SRR765688.32089 HQP9GKT01AO7NN length=385 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTT............GACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAA......GACTCTGATACCAGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 253 41 253 1 317 27 301 7 22 7 9 317 34 15 34 54 TGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGG 296.0 0.8734999999999999 2e-82 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3 60.0 0.9706 2e-13 16AT17 ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCT CTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTC AGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACAACTTT............GACACCTATTGG ATCTATCCTGAA......GACTCTGATACC GTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTAT F,RC,F MID54 IgG-PCR LR2 2
SRR765688.13860 HQP9GKT01AT8BJ length=362 NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGGAAGGACCTTCTACAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAGCCTCCAC F T T T IGHV6-1*01,IGHV6-1*02 IGHD2-2*01,IGHD2-2*02,IGHD2-2*03 IGHJ4*02 CTGTGTCATCTCCGGGGACAGTGTCGCCGGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGG-AAGGACCTTCTACAGGTCCAAGTGGTATAATGAATACGCACTGTCTTTGAAAAGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG .................................................................CTGTGTCATCTCCGGGGACAGTGTCGCC......GGCAACAGTGTTGCTTGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGG-AAGGACCTTCTACAGGTCC...AAGTGGTATAATGAATACGCACTGTCTTTGAAA...AGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG 20 240 63 241 1 318 8 268 9 10 9 4 281 46 3 46 45 TGTGCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTACTGG 299.0 0.9046 2e-83 5TC19GT1CTGA9TC13CA13CA18TC3-G6CA1TA24AT2CT3CG1GA3TG11GA1CA9GC31AG30TG1TG5TC9 75.8 0.9565 3e-18 17TC6TA21 .................................................................CTGTGTCATCTCC TGGAACTGGCTCAGGCAGTCCCCCTCGAGAGGCCTTGAGTGGTTGG-AAGG GAATACGCACTGTCTTTGAAA...AGTCGAGTCACCATCAACGCAGACACATCCAAGAACCAGTTCTCCCTGCAACTGAACTCTGTGACTCCCGAGGACACGGCTTTTTATTATTGT TGGGGTCAGGGATCCCTGGTCACCGTCTCCTCAG GGGGACAGTGTCGCC......GGCAACAGTGTTGCT ACCTTCTACAGGTCC...AAGTGGTATAAT GCAAGAACTAGATATAGTAGTACGCCTTACTTTGACTAC F,RC,F MID53 IgG-PCR LR1 2
SRR765688.20740 HQP9GKT01ANHJ2 length=378 NNNNNNNNNNNNNNNNNNNNAAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC T T F F IGHV1-58*01 IGHD4-23*01 IGHJ6*02,IGHJ6*04 AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTTACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGCAGTGGTCACACAAACTACGCACAGAAGTTCCAGGAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC .......................................AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCTGGATTCAGCTTT............ACTAGTTCTGCTGTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGGATCGTCGTCGGC......AGTGGTCACACAAACTACGCACAGAAGTTCCAG...GAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGTGCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC 21 260 37 260 1 320 3 284 12 4 12 25 321 18 18 18 69 TGTGCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGGGGC 341.0 0.9192 5e-96 26TC2TCCA15GC9TC20CA3AG4CT5GA17TA11CT9CA36GC5TCTC16TGTCTA3TC4CA43TC11 36.2 1.0 2e-06 18 .......................................AAGCCTGGGACCTCAGTGAAGGTCTCTTGTCAGGCTTCT GTGCAGTGGGTGCGCCAGACTCGCGGACAGCGCCTTGAGTGGATAGGTTGG AACTACGCACAGAAGTTCCAG...GAAAGAGTCAGCATTATTAGGGACATGTCCACAATTTCAGTCTACCTGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTATTGT GGC GGATTCAGCTTT............ACTAGTTCTGCT ATCGTCGTCGGC......AGTGGTCACACA GCGGCAGAAGGCTACGGTGGTAATCCCCTACAGTATTATAATAATGAAGGTATGGACGTCTGG F,F,F MID53 IgG-PCR LR9 2
SRR765688.15114 HQP9GKT01DWQ8O length=372 NNNNNNNNNNNNNNNNNNNNATTGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCACGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCACAAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC F T T T IGHV2-70*13 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ6*02 TGACCTGCTCCTTCTCTGGGTTCTCACTCACCGCCACTGGAATGTGTGTGACCTGGATCCGTCAGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGATGATGATAAATATTACAACACATCTCTGAAGACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA .............................................................TGACCTGCTCCTTCTCTGGGTTCTCACTCACC......GCCACTGGAATGTGTGTGACCTGGATCCGTCAGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTCATTGATTGGGAT.........GATGATAAATATTACAACACATCTCTGAAG...ACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 23 237 59 238 1 317 11 271 6 8 6 1 278 58 5 58 54 TGTGCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTCTGG 315.0 0.9372 4e-88 8TA21CG1GA1CT1CG14CG12C-18TG11GA26TC4AG40AT1-C-A52AG14 99.6 0.9655 2e-25 11TA2CG43 .............................................................TGACCTGCTCCTTCTCT GTGACCTGGATCCGTCAGCCCCCAGGGAAGGCCCTTGAGTGGCTTGCGCTC TATTACAACACATCTCTGAAG...ACCAGGCTCACCATCTCCAAGGACACCAC--AAAACCAGGTGGTCCTTACAATGACCAACATGGACCCTGTGGACACAGCCACATATTATTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGGTTCTCACTCACC......GCCACTGGAATGTGT ATTGATTGGGAT.........GATGATAAA GCACGTGTTTACGGGGCTGGAATCTACTACTACTTCGCTATGGACGTC F,RC,F MID54 IgG-PCR LR8 2
SRR765688.44373 HQP9GKT01A960E length=371 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*01,IGHV4-59*07 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ6*02 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA ....................................................CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATG............AGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAAT.........GGGGTCACCAACTACAGCCCCTCCCTCAAG...AGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 21 241 50 241 1 317 8 270 6 16 6 7 283 52 11 52 51 TGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGG 318.0 0.9212 5e-89 33TC3GC2GT3GT10TC42CT10TC1AG4GATG11GA18GA3GA9CT31TC2CA4CT1CT21AG14 87.7 0.9615 8e-22 8CG1TA41 ....................................................CCCTGTCCCTCACCTGCACTGTCTCT TGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCAT AACTACAGCCCCTCCCTCAAG...AGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGTGGCTTCATG............AGGAGTGACTAC ATCTATTATAAT.........GGGGTCACC GCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTC F,RC,F MID54 IgA-PCR LR7 3
SRR765688.22020 HQP9GKT01BU60O length=404 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD1-1*01 IGHJ6*02 AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTC............ACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGC......ACTGGGGACCCAACGTATGCCCAGGCCTTTACA...GGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA 21 263 34 263 1 320 15 299 9 3 9 14 322 47 16 47 66 TGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGG 339.0 0.9125 2e-95 26CT16CG5TCTC5AT1AGGC6CA3CA24GA30GAGA7GA18CG3TC8TG11TC1CT30CG6GA1TC23TG5TC11 77.8 0.9573999999999999 8e-19 5TA23GA17 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCT CTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGG ACGTATGCCCAGGCCTTTACA...GGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGT TGGGGCCAAGGGACCGCGGTCACCGTCTCCTCA GCATACATTTTC............ACAAAGTATGCT ATCAACACCGGC......ACTGGGGACCCA GCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTC F,RC,F MID54 IgG-PCR LR3 2
SRR765688.23018 HQP9GKT01DGIK6 length=395 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC T T F T IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCACCAAGTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG ....................................................CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCC............GCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGT.........TGGGGCACCAAGTACAACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG 21 255 50 243 1 319 0 276 6 3 6 28 310 44 5 44 57 TGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGG 230.0 0.7961 1e-62 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3G-C-A-A-G-T-C-C-G-A-C-C-4GC18GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11 63.9 0.9318000000000001 1e-14 23TC2GT10GT6 ....................................................CCCTGTCCCTCACCTGCAGTATCTCT TGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTT AAGTACAACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGT TGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG GGCGGCTCCGCC............GCCCACTACTAC GCCTATGTAGGT.........TGGGGCACC GCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTAC F,RC,F MID54 IgG-PCR LR7 3
SRR765688.46548 HQP9GKT01BMT60 length=377 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD1-1*01,IGHD1-20*01,IGHD1-7*01 IGHJ4*02 AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTC............ACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAC......ACTGGGAACCCAACATATGCCCAGGCCTTCACA...GGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 263 34 263 1 320 11 295 6 7 6 0 301 42 7 42 39 TGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGG 349.0 0.924 2e-98 12AG5TG5CG11AG1CT9TA9AG84AG10CG25CT16TC3AG8GC6AG2AG12AG4GC4AG1AG10GC6 83.8 1.0 1e-20 42 ....................................AAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCT ATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGG ACATATGCCCAGGCCTTCACA...GGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACTCCTTC............ACTAACTATGCT ATCAACACCAAC......ACTGGGAACCCA GGGAGAGAGAGTCTCGGCCACTGGATTGACTAC F,F,F MID54 IgG-PCR LR3 5
SRR765688.11345 HQP9GKT01A2AIW length=375 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG F T T T IGHV4-59*08 IGHD5/OR15-5a*01,IGHD5/OR15-5b*01 IGHJ5*02 CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAA-CCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCC-TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATC............AGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATT.........GAGACCACCTATTACAA-CCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCC-TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 243 50 244 1 320 13 277 7 9 7 4 288 46 5 47 51 TGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCC-TGG 300.0 0.9016 7e-84 40ATGA1AT5TC18TC27CGAG9GT3TG2AG2CG4TA1TC5-C31CTGA14TC5TC7GA5AGTC3CG25CT1TA12 77.8 0.9787 7e-19 12-C34 ....................................................CCCTGTCCCTCACCTGCACTGTCTCT TGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATAT TATTACAA-CCCTCCCTCAAG...AGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGGCTCCATC............AGAGGATACTAT ATCTATGACATT.........GAGACCACC GCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCC- F,RC,F MID53 IgA-PCR LR7 2
SRR765688.36594 HQP9GKT01B0W9F length=368 NNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGACGCAGTCGGGATCACGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV7-4-1*01,IGHV7-4-1*02 IGHD3-10*01,IGHD3-10*02,IGHD6-19*01 IGHJ4*02,IGHJ5*02 AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCCACTGGGAATTCAAATTTCGCCCAGGACTTCACAGGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGACGCAGTCGGGATCACGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTC............ACTACTTCTGCTATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGGATCAACACCGCC......ACTGGGAATTCAAATTTCGCCCAGGACTTCACA...GGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGTGCGACGCAGTCGGGATCACGCCTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 259 34 259 1 316 1 281 5 8 5 13 299 35 14 35 30 TGTGCGACGCAGTCGGGATCACGCCTCTGG 308.0 0.8803 3e-86 58CGTC1CA6AG14CG14CG3AG23GACA9TCTC3ACTG1TACT7AG12CG12GT5TA20TC2GC1AG3CTTG2CGTC2CA3CG4AG9AG1TG13 69.9 1.0 2e-16 35 ....................................AAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCT ATAAATTGGGTGCGACACGCCCCTGGACAAGGCCTTAAGTGGATGGGATGG AATTTCGCCCAGGACTTCACA...GGACGCTTTGTCTTCTCCGTGGACTCCTCTGTCAGCACGGCATATTTGGAAATCCTCACTCTCAAGCCTGAAGACACTGCCATTTATTACTGT TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGATACACCTTC............ACTACTTCTGCT ATCAACACCGCC......ACTGGGAATTCA GCGACGCAGTCGGGATCACGCCTC F,RC,F MID54 IgA-PCR LR3 2
SRR765688.47496 HQP9GKT01DBCHA length=377 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG T T F F IGHV4-34*01,IGHV4-34*02 IGHD3-9*01 IGHJ4*02 CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTC............AGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGT.........GGAAGCACCAACTACAACCCGTCCCTCAAG...AGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 21 241 50 241 1 317 12 274 12 19 12 13 299 43 6 43 57 TGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGG 299.0 0.8963 2e-83 45TA1GTCA5CG9TC3TC9TA18AG2TACA43GT7GC1TA1TAAT8TA7AG1GATC2CG10GA5CGTC26CG8CT5 69.9 0.9534999999999999 2e-16 7TAGC34 ....................................................CCCTGTCCCTCACCTGCGCTGTCTAT TGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGAT AACTACAACCCGTCCCTCAAG...AGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGC TGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG GGTGGGTCCTTC............AGTGGTTTCGCC CTCAATCATAGT.........GGAAGCACC GCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTG F,RC,F MID53 IgA-PCR LR7 5
SRR765688.46707 HQP9GKT01BT4GG length=359 NNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV6-1*01,IGHV6-1*02 IGHD5-12*01 IGHJ4*02 CTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG .................................................................CTGTGTCATCTCCGGCGACAGTGTCTCT......AGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCC...GAGTGGTATAATGATTATGCCTTATCTATGAAG...AGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG 20 243 63 243 1 320 8 271 8 7 8 6 285 40 9 40 42 TGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGG 265.0 0.8477 4e-73 5TC9CG14TCGA4ATAGTC7CA5GA7AG36ATTAGCCT8GA19CATG5AG4GA8TA1TCTC2TC1GAGC8GA4GA7CT3TCTC5AGTC3CA25AG1TG5TC6GA4 71.9 0.975 4e-17 19TC20 .................................................................CTGTGTCATCTCC TGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGG GATTATGCCTTATCTATGAAG...AGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGT TGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG GGCGACAGTGTCTCT......AGTGACAGAATTGCT ACAATGCACAGGTCC...GAGTGGTATAAT GCAGGAGACAGGTATAATAGTGGCGGCGGAGACTAC F,F,F MID53 IgG-PCR LR1 2
SRR765688.35420 HQP9GKT01C0N6S length=397 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC T T F F IGHV5-51*01 IGHD3-16*02,IGHD3-3*01,IGHD3-3*02 IGHJ6*02,IGHJ6*04 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTCAGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGATGAGTTTGATATTAGATATAGCCAGTCCTTCCAGGGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCTGGATACAGGTTC............AGCCACTATTGGATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATGATCTATCCTGAT......GAGTTTGATATTAGATATAGCCAGTCCTTCCAG...GGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGTGCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC 21 256 41 256 1 320 29 306 7 30 7 6 319 28 8 28 81 TGTGCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGGGGC 313.0 0.8906000000000001 1e-87 15GA15CT11GC2CT1GC1CAAG3TC7CG41CGCG2GC10AG3GC1TC5TCTC5TC4AC9GA19TC24TCAC9AG20GC6AG4TA9GA2 48.1 0.9643 7e-10 12GT15 ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGGCTCT ATCGCCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGCCATG AGATATAGCCAGTCCTTCCAG...GGCCAGGTCACCATCTCAGTCGACAAGTCCATCAGCACCGCCTATATGCAGTGGAACAGCCTGAAGGCCTCGGACAGCGCCATATATTTCTGT GGC GGATACAGGTTC............AGCCACTATTGG ATCTATCCTGAT......GAGTTTGATATT GCGAGGCATGGATTATACGGTTGTGATCATACCGGCTGTTATACAAGCTTCTACTACTACGGGATGGACGTCTGG F,F,F MID54 IgG-PCR LR5 2
SRR765688.35321 HQP9GKT01C64W5 length=377 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAGCCTCCAC F T T T IGHV5-10-1*01,IGHV5-10-1*03 IGHD4-17*01 IGHJ4*02 CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTTACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTAATCCCAGTGACTCTGATACCAAGTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCTGGATACAGTTTT............ACGAGTTATTGGATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTAATCCCAGT......GACTCTGATACCAAGTACAGCCCGTCCTTCCAA...GGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGTGCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCACTGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG 21 254 41 253 1 317 9 284 14 1 14 12 310 36 13 36 48 TGTGCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCACTGG 332.0 0.9251999999999999 3e-93 30CG12TC5GC2TC2TC5GC1CG12AGTA29G-6AG4CT9GT7GC37TC14CG20CG2AG30TC8 63.9 0.9722 1e-14 24TC11 ...........................................CCGGGGAGTCTCTGAGGATCTCCTGTAAGGCTTCT ATGACCTGGGTGCGCCAATTGCCCGGGAAAGGCCTGGAGTGGATGGGGAGG AAGTACAGCCCGTCCTTCCAA...GGCCACGTCACCATCTCAGTTGACAAGTCCATCACCACTGCCTACCTGCAGTGGACCAACCTGAAGGCCTCGGACACCGCCATGTATTATTGT TGGGGCCAGGGAACCCTGGTCATCGTCTCCTCAG GGATACAGTTTT............ACGAGTTATTGG ATTAATCCCAGT......GACTCTGATACC GCGAGCCGCTCGGGTGACTACGGTGACTTCGAGTTTGCCCAC F,F,F MID54 IgG-PCR LR5 2
SRR765688.40878 HQP9GKT01B4JCW length=380 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAGCATCCCCG T T F F IGHV4-59*01,IGHV4-59*07 IGHD2-8*01 IGHJ4*02 CCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATCAATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGTGGGACCACCAACTACAACCCGTCCCTCAAGAGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG ....................................................CCCTGTCCCTCACCTGCACTATCTCTGGTGGCTCCATC............AATGGTTACTACTGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTATATTCATTATAGT.........GGGACCACCAACTACAACCCGTCCCTCAAG...AGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTATTGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG 21 242 50 242 1 318 8 271 11 4 11 18 300 45 4 45 60 TGTGCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTATTGG 322.0 0.9256 2e-90 20AG18AG1GA12CG13TC6AG18AT8TCCT4TC7CG15GC14GA14GA28GA1TC29CG1AG15 73.8 0.9556 1e-17 10TC21TA12 ....................................................CCCTGTCCCTCACCTGCACTATCTCT TGGACCTGGATCCGGCAGTCCCCAGAGAAGGGACTGGAGTGGATAGGGTAT AACTACAACCCGTCCCTCAAG...AGTCGGGTCACCATATCAGTGGACACGTCCAAGAACCAGTTCTCCCTGAGGTTGAGCTCTGTGACCGCTGCGGACACGGCCCTATATTACTGT TGGGGCCAGGGAACCCTGGTCTCCGTCTCCTCAG GGTGGCTCCATC............AATGGTTACTAC ATTCATTATAGT.........GGGACCACC GCGAGAATTAGGACATATTGTACTACTGACAGTTGTTCTTTCGACTTTGACTAT F,RC,F MID54 IgA-PCR LR7 3
SRR765688.44256 HQP9GKT01DI3B9 length=395 NNNNNNNNNNNNNNNNNNNGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV3-30-3*02 IGHD1-14*01 IGHJ6*02 GGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA .....................GGGGGA...GGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC............AGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGAT......GGAAGCACTGGATACTACGCAGACTCCGTGAAG...GGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCA 20 275 22 275 1 320 3 298 7 10 7 14 319 43 20 43 48 TGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGG 364.0 0.9236 5e-103 50CG19CGTCCT2TGGC1GA1AG50GA3ATTA11CA1GAGA39TC19TC7TC18CG1AG4AG11AG17 85.7 1.0 3e-21 43 .....................GGGGGA...GGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCT GTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTT TACTACGCAGACTCCGTGAAG...GGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGT TGGGGCCAAGGGACCACGGTCACCGTCTCCTCA GGATTCACCTTC............AGTACTCATTGT ATGTCAATTGAT......GGAAGCACTGGA GCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTC F,F,F MID54 IgG-PCR LR6 2
SRR765688.42968 HQP9GKT01B3HAW length=382 NNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG T T F F IGHV1-69*01,IGHV1-69*12,IGHV1-69D*01 IGHD6-13*01 IGHJ6*02 CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTC............AGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATC......GTTGGTACAGCAAAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCC 21 255 41 255 1 319 7 283 8 10 8 2 293 51 9 51 51 TGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGG 339.0 0.9255 1e-95 43GC6CAAG70GT13GC10GAAG8TC3CG4TG3TA5CG13AC2CA3GT4CG16CG6CG11TG16 93.7 0.9804 1e-23 38AG12 ...........................................CTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCT ATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGG AAGTACGCACAGAGATTCCAG...GGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGT TGGGGCCAAGGGACCACAGTCACCGTCTCC GGAGGCACGTTC............AGCCACTATGCT ATCATCCCTATC......GTTGGTACAGCA GCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTC F,F,F MID54 IgA-PCR LR2 5
SRR765688.43118 HQP9GKT01AR4EN length=394 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCAAGTACAACCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAGCCTCCAC F T T T IGHV4-59*02 IGHD2-15*01,IGHD2-2*01,IGHD2-2*02 IGHJ4*02 CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCCGCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGTTGGGGCAGCAAGTCCGACCCCTCCCTCAAGGGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG ....................................................CCCTGTCCCTCACCTGCAGTATCTCTGGCGGCTCCGCC............GCCCACTACTACTGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTTGCCTATGTAGGT.........TGGGGCAGCAAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG 21 254 50 243 1 319 0 275 6 3 6 28 309 44 5 44 57 TGTGCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTACTGG 223.0 0.7874 2e-60 18GC1AG7CT7CT1GACGCTCAAGCT11TC5TC6TC2GC24CGTG1TA1GACT4GTTAACGA2TG2GA3GC3GC1CA1GA5A-A-G-T-A-C-A-A-C-C-C-9GA13GA3AGCT3TCGA6GA1GA7GC9TC3CG25CG1TG4TA11 63.9 0.9318000000000001 1e-14 23TC2GT10GT6 ....................................................CCCTGTCCCTCACCTGCAGTATCTCT TGGAGTTGGATTCGGCAGTCCGCAGGGAAGGGACTGGAGTGGATTGCTTTT AAGTCCGACCCCTCCCTCAAG...GGTCGAGTCACCATGTCAACAGATGCGTCCAGGGACCAGTTGTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCCTTTATTTCTGT TGGGGCCAGGGAATCCGGGTCACCGTCGCCTCAG GGCGGCTCCGCC............GCCCACTACTAC GCCTATGTAGGT.........TGGGGCAGC GCGAGAGGATATTCCTACGACCTCGATGGCAATTATGGTGGCTTTGACTAC F,RC,F MID54 IgG-PCR LR7 4
SRR765688.21808 HQP9GKT01A2WPP length=385 NNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC T T F F IGHV5-51*01 IGHD3-10*01,IGHD3-10*02,IGHD3-16*01 IGHJ4*02,IGHJ5*02 CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATACACTTCGTCCTTCCACGGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTT............GACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAA......GACTCTGATACCAGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTC 21 253 41 253 1 317 27 301 7 22 7 9 317 32 15 32 54 TGTGTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTATTGG 296.0 0.8734999999999999 2e-82 16AG14GT10AG4GAAC2CG3TC3CA18CT5AG12AG8TGCA12AGAT19CGTCTC10CA5CG8TC10GA4GA4TC4GC7GC7TC7GA2TC8GC6AG10TC3 56.0 0.9688 3e-12 16AT15 ...........................................CCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCT CTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTC AGATACACTTCGTCCTTCCAC...GGCCACGTCACCATTTCAGCCGACAGGTCCGTCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGGTCTCGGACAGCGCCATATATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTC GGATACAACTTT............GACACCTATTGG ATCTATCCTGAA......GACTCTGATACC GTGAGGAATATAAGGCGCTCCGATAATACTGCTTATTATGCCGAGTAT F,F,F MID54 IgG-PCR LR2,LR5 9
SRR765688.39792 HQP9GKT01C51DY length=373 NNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC T T F F IGHV7-4-1*02 IGHD6-25*01 IGHJ4*02 AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCTGGATACACCTTC............ACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAA......ACTGGGAACCCAACCTATGCCCAGGACTTCACA...GGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG 21 261 34 261 1 318 7 289 6 8 6 5 300 40 9 40 36 TGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGG 333.0 0.9079999999999999 1e-93 4GA19AG11AG1GT18TACG4TG2GA46CA5TCCA5CA1AC14CG10AG19CT28GA6GC10TC6AG4AG5CT3AG1TG15 63.9 0.95 1e-14 4TA17AT17 ....................................AAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACGTCT GTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGG ACCTATGCCCAGGACTTCACA...GGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCGTATCTGGAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGT TGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAG GGATACACCTTC............ACTTCCTATTCT ATTCACACCCAA......ACTGGGAACCCA GCGAGACTCGACGGCAGCGATAGGGACTTC F,RC,F MID54 IgG-PCR LR3 2
SRR765688.39546 HQP9GKT01D90AI length=383 NNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCAGGGAAGTTCGATCACCGTCTCCTCAGCCTCCAC T T F F IGHV4-34*01,IGHV4-34*02 IGHD3-22*01,IGHD3-3*01,IGHD3-9*01 IGHJ1*01 CCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTCAGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGTGGAGACATCAACTACAACCCGTCCCTCATCAGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCAGGG ....................................................CCCTGTCCCTCACCTGCGTTGTCCATGGTGCGTCGTTC............AGTGATTATTATTGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAAATCCATGATAGT.........GGAGACATCAACTACAACCCGTCCCTCATC...AGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCAGGG 21 241 50 241 1 317 30 292 7 22 7 15 314 11 19 11 71 TGTGCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCAGGG 271.0 0.8589 6e-75 18TC4CT6CG3GC7AG3TC2TC4CGAC8GC3TC4TA14AG8AG6CA2GC8GAAG2TC20TACG5CACG7CA23AG9CAGA4GACG19TA3GC1AG1TG2CT11 22.3 1.0 0.039 11 ....................................................CCCTGTCCCTCACCTGCGTTGTCCAT TGGACATGGATCCGGCAGTCCCCTGGGAAGGGGCTGGAATGGATTGGAGAA AACTACAACCCGTCCCTCATC...AGTCGCCTCACCATCTCAGTAGACACGTCCAAGAACCAATTCTCCCTGCGGCTGGCCTCTGTGACCGCCGCGGACTCGGGTATTTACTACTGT GGG GGTGCGTCGTTC............AGTGATTATTAT ATCCATGATAGT.........GGAGACATC GCGAGGGTGAGCGTCCGATTGGACACTACGGGCTATTATTACGGGTCCCTAGATTATTGGGGCCA F,RC,F MID53 IgG-PCR LR7 2
Raw
SRR765688_migmap.txt
freq count v d j cdr3nt cdr3aa mutations.nt.FR1 mutations.nt.CDR1 mutations.nt.FR2 mutations.nt.CDR2 mutations.nt.FR3 mutations.nt.CDR3 mutations.nt.FR4 mutations.aa.FR1 mutations.aa.CDR1 mutations.aa.FR2 mutations.aa.CDR2 mutations.aa.FR3 mutations.aa.CDR3 mutations.aa.FR4 cdr.insert.qual mutations.qual v.end.in.cdr3 d.start.in.cdr3 d.end.in.cdr3 j.start.in.cdr3 v.del d.del.5 d.del.3 j.del pol.v pol.d.5 pol.d.3 pol.j has.cdr3 in.frame no.stop complete canonical contignt
0.05128205128205128 2 IGHV1-46*01 IGHD1-1*01 IGHJ6*01 TGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGG CARDSLHDYGMDVW S65:C>T S329:G>C,S349:T>C S21:C>C S109:G>G,S116:V>A HHIIIIII IBI 11 19 24 22 0 12 0 12 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTTTCCTGTAAGGCATCTGGATACACCTTCACCAGCTACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAACCCTAGTGGTGGTAGCACAAGCTACGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGACACGTCCACGAGCACAGTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGCCTCCTCAGCATCCCCG
0.05128205128205128 2 IGHV1-18*01 IGHD2-2*01 IGHJ4*01 TGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGG CARDRGSTRWLEPIDYW S67:A>C S92:C>T,S95:T>C S145:G>C S168:A>G S176:C>G,S188:G>A,S189:C>T,S222:T>C,S259:G>T,S278:G>A,S284:C>T S341:A>G S22:K>T S30:S>S,S31:Y>Y S48:G>A S56:N>D S58:N>K,S62:K>K,S63:L>F,S74:S>P,S86:R>I,S92:V>V,S94:Y>Y S113:Q>Q IIIIIFFIIIFFFHICCCH IIIIIHCIHIIIC 11 16 26 40 0 11 10 6 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCACGGCTTCTGGTTACACCTTTACCAGTTACGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGCATGGATCAGCGCTTACAATGGTGACACAAAGTATGCACAGAAATTCCAGGGCAGAGTCACCATGACCACAGACACACCCACGAGCACAGCCTACATGGAGCTGAGGAGCCTGATATCTGACGACACGGCCGTATATTATTGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV7-4-1*01 IGHD2-15*01 IGHJ5*01 TGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGG CARDPNAIVVEDWFDPW S57:G>A,S71:T>C S81:A>C,S92:C>T S161:C>G S176:G>A,S181:C>T,S187:G>A,S213:T>C,S243:C>G,S249:T>A,S258:A>G,S263:T>C,S283:A>T S330:T>C S341:A>G,S350:G>A S19:V>I,S23:A>A S27:T>P,S30:S>S S53:N>K S58:T>T,S60:A>V,S62:G>D,S71:L>L,S81:Q>E,S83:C>S,S86:K>E,S87:A>A,S94:Y>F S110:S>P S113:Q>Q,S116:L>L IIIIIIIIIIIIIIII II>CIIIIHIIII?>HI 9 22 31 34 0 9 13 3 12 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCCTCTGGATACCCCTTCACTAGTTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAAGACTGGGAACCCAACATATGTCCAGGACTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGCACGGCATATCTGGAGATCAGCAGCCTAGAGGCCGAGGACACTGCCGTGTATTTCTGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGGGGCCAGGGAACCCTAGTCACCGTCTCCGCAGCATCCCCG
0.02564102564102564 1 IGHV7-4-1*01 IGHD6-25*01 IGHJ4*01 TGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGG CARLDGSDRDFW S37:A>G,S57:G>A,S69:G>A S90:A>T,S91:G>C,S96:G>T S99:A>G,S146:A>C S152:C>T,S153:A>C,S159:A>C,S161:C>A S176:G>C,S187:G>A,S207:T>C,S249:T>A,S254:C>T,S261:G>A,S266:G>A,S272:T>C,S276:G>A,S278:G>T S316:A>T S326:A>G,S334:T>A S12:K>R,S19:V>I,S23:A>T S30:S>S,S30:S>S,S32:A>S S33:M>V,S48:G>G S50:I>I,S51:N>H,S53:N>Q,S53:N>Q S58:T>T,S62:G>D,S69:F>L,S83:C>S,S84:S>S,S87:A>T,S88:E>E,S90:T>T,S92:V>I,S92:V>I S105:Y>F S108:Q>Q,S111:L>Q IIIIIIIIIIHH BIIIIHIIIIGH?IIIIIIIIHIHI 9 16 22 27 0 7 5 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAGGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACACCTTCACTTCCTATTCTGTGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATTCACACCCAAACTGGGAACCCAACCTATGCCCAGGACTTCACAGGACGGTTTGTCCTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGTCTAAAGACTGAAGACACCGCCATTTATTACTGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD3-10*01 IGHJ5*01 TGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGG CARDLNYIHFDPW S36:A>G,S55:A>C,S57:G>A,S72:T>A,S73:C>T S76:G>A,S82:C>A,S88:C>A,S89:T>A,S90:A>T,S92:C>T,S94:A>G,S96:G>A,S97:C>G S101:G>A,S108:G>C S155:C>T,S156:A>G,S157:C>T,S161:C>T,S165:G>A,S167:G>A,S169:A>G,S170:C>G S176:G>A,S184:A>C,S192:A>G,S200:G>A,S219:A>G,S234:G>A,S236:A>T,S249:T>A,S253:G>A,S257:A>C,S261:G>A,S266:G>A,S278:G>T,S284:C>T S318:T>C S329:A>G S12:K>E,S18:K>T,S19:V>I,S24:S>I,S24:S>I S25:G>E,S27:T>N,S29:T>K,S29:T>K,S30:S>C,S30:S>C,S31:Y>C,S32:A>S,S32:A>S S33:M>I,S36:V>L S51:N>N,S52:T>V,S52:T>V,S53:N>N,S55:G>R,S55:G>R,S56:N>R,S56:N>R S58:T>T,S61:Q>P,S64:T>A,S66:R>R,S73:T>A,S78:A>T,S78:A>T,S83:C>S,S84:S>N,S85:L>L,S87:A>T,S88:E>E,S92:V>V,S94:Y>Y S106:S>P S109:Q>Q IIIIIHHIIIII EIIIIIIGHIIIII?IHIIII???ICIIIIIIIIIIHIAH 9 16 22 27 0 23 2 8 13 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGAGCCTGGGGCCTCAGTGACGATTTCCTGCAAGGCTATTGAATACAACTTCAAATGTTGTAGTATAAATTGGCTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAATGTCAATACTAGAAGGCCAACATATGCCCCGGGCTTCGCAGGACGATTTGTCTTCTCCTTGGACGCCTCTGTCAGCACGACTTATCTGCAGATCAGCAACCTCAAGACTGAAGACACTGCCGTTTATTATTGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV5-10-1*01 IGHD6-13*01 IGHJ4*01 TGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGG CARHDSSSWYVQVDYW S338:A>G S112:Q>Q IIIIIIIIIII H 9 13 30 37 0 4 0 6 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAGGATCTCCTGTAAGGGTTCTGGATACAGCTTTACCAGCTACTGGATCAGCTGGGTGCGCCAGATGCCCGGGAAAGGCCTGGAGTGGATGGGGAGGATTGATCCTAGTGACTCTTATACCAACTACAGCCCGTCCTTCCAAGGCCACGTCACCATCTCAGCTGACAAGTCCATCAGCACTGCCTACCTGCAGTGGAGCAGCCTGAAGGCCTCGGACACCGCCATGTATTACTGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV4-39*01 IGHD1-7*01 IGHJ4*01 TGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGG CARQWDDTGNYYARAHFDSW S66:A>G S79:G>A,S100:A>G S151:G>C D162:TAT,S173:C>T S182:C>T,S212:A>G,S224:G>C,S248:G>C,S254:C>G,S281:G>A S343:A>C,S344:C>T S353:A>G S22:T>A S26:G>D,S33:Y>C S50:G>A D54:Y,S57:S>S S60:Y>Y,S70:I>M,S74:T>T,S82:K>N,S84:S>R,S93:V>V S114:Y>S,S114:Y>S S117:Q>Q IIHHHIIIIIIDD;IHIIHHHHHHIII IIIIIIIIIIIIHE? 11 25 33 46 0 9 0 3 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTGCTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGCGAGTATCTATAGTGGGAGTACCTACTATAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTAGACACCTCCAAGAACCAGTTCTCCCTGAACCTGAGGTCTGTGACCGCCGCAGACACGGCTGTATATTACTGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV5-51*01 IGHD3-10*01 IGHJ4*01 TGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGG CVRNIRRSDNTAYYAEYW S56:G>A,S71:T>G S82:G>A,S87:A>G,S88:C>A,S91:G>C,S95:C>T S99:A>C,S118:T>C,S124:G>A,S137:G>A,S146:G>T,S147:A>C S160:G>A,S161:T>A S179:C>T,S181:G>C,S182:C>T,S183:C>T,S194:A>C,S200:G>C,S209:C>A,S220:A>G,S230:C>T,S235:C>G,S243:C>G,S251:C>T,S259:A>G,S261:G>A,S262:C>T,S271:C>G,S278:G>A S289:C>T S344:A>G,S352:T>A S18:K>K,S23:G>G S27:S>N,S29:T>D,S29:T>D,S30:S>T,S31:Y>Y S33:I>L,S39:M>T,S41:G>E,S45:E>E,S48:G>G,S49:I>L S53:G>E,S53:G>E S59:Y>Y,S60:S>T,S60:S>T,S61:P>S,S64:Q>H,S66:Q>H,S69:I>I,S73:K>R,S76:S>S,S78:A>G,S81:Q>E,S83:S>S,S86:K>R,S87:A>I,S87:A>I,S90:T>S,S92:M>I S96:A>V S114:Q>Q,S117:L>Q IIIIIIIIIIIIIIIIIIIIIIIIIIIIHHHII @AIIIIHII=III??IIIIIIIIIIIIIIIIIIHI 8 35 42 48 3 21 3 11 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCGGGGAGTCTCTGAAAATCTCCTGTAAGGGGTCTGGATACAACTTTGACACCTATTGGCTCGGCTGGGTGCGCCAGACGCCCGAGAAAGGCCTGGAATGGATGGGTCTCATCTATCCTGAAGACTCTGATACCAGATATACTTCGTCCTTCCACGGCCACGTCACCATATCAGCCGACAGGTCCATCAGTACCGGCTACCTGGAGTGGAGTAGCCTGAGGATCTCGGACAGCGCCATATATTACTGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCTGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD6-19*01 IGHJ4*01 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG CARGRWLSRPAKDYW S57:G>A,S69:G>A S82:C>T,S83:C>G,S90:A>T,S91:G>C,S92:C>G,S96:G>T S104:T>C,S118:C>T,S121:C>G,S122:T>C S175:C>G,S176:G>T,S179:T>C,S181:C>T,S184:A>C,S188:C>A,S213:T>G,S215:G>C,S230:C>G,S243:C>G,S249:T>A,S257:A>T,S266:G>A,S278:G>A,S284:C>T S335:A>G,S343:T>A S19:V>I,S23:A>T S27:T>M,S27:T>M,S30:S>S,S30:S>S,S30:S>S,S32:A>S S34:N>N,S39:A>V,S40:P>R,S40:P>R S58:T>S,S58:T>S,S59:Y>Y,S60:A>V,S61:Q>P,S62:G>G,S71:L>V,S71:L>V,S76:S>R,S81:Q>E,S83:C>S,S85:L>L,S88:E>E,S92:V>V,S94:Y>Y S111:Q>Q,S114:L>Q HHHIIIIIIIIIIIIIIIII HIIIIIIIIIIIIIIIH?IIIIIHIIIHI 9 14 21 36 0 10 4 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGGACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV4-59*01 IGHD5/OR15-5a*01 IGHJ5*01 TGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGG CARHSGPNSVLFWFDPW S89:T>A,S90:A>G,S92:T>A,S98:C>T S117:C>T,S145:G>C,S146:G>A S156:T>G,S160:G>T,S163:G>A,S166:G>C S171:A>T,S173:C>T,S211:T>C,S212:A>G,S227:C>T,S233:C>T,S241:A>G,S247:G>A,S248:C>T,S252:G>C,S260:T>C,S263:G>A,S278:T>C,S280:A>T S327:T>C S338:A>G S29:S>R,S30:S>G,S30:S>G,S32:Y>Y S39:P>S,S48:G>A,S48:G>A S52:Y>D,S53:S>I,S54:G>E,S55:S>T S57:N>Y,S57:N>Y,S70:V>A,S70:V>A,S75:N>N,S77:F>F,S80:K>R,S82:S>N,S82:S>N,S84:V>L,S86:A>A,S87:A>A,S92:Y>Y,S93:Y>F S109:S>P S112:Q>Q IIIIIIHHHHHHIIIIIII DCIIIIIHIIIIIIII>IIIIIIII@G 9 24 31 35 2 8 8 4 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCAGAGGATACTATTGGAGCTGGATCCGGCAGTCCCCAGGGAAGGGACTGGAGTGGATTGCATATATCTATGACATTGAGACCACCTATTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCGGACACGTCCAAGAATCAGTTTTCCCTGAGGCTGAATTCTCTGACCGCCGCAGACACGGCCGTGTACTTCTGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV2-5*01 IGHD3-16*01 IGHJ4*01 TGTGCACACAGTGCGGGATGGCTGCCTGATTACTGG CAHSAGWLPDYW S77:G>A,S89:C>T,S97:G>C S107:G>A,S108:G>C,S109:G>A S165:A>G,I172:CCAAAT,S174:A>T,S176:G>C S177:C>A,S180:T>A,S181:A>C,S232:A>G,S240:G>A,S245:T>C,S268:T>C S329:A>G,S350:C>T S25:G>G,S29:S>S,S32:G>A S35:V>V,S36:G>H,S36:G>H S55:N>D,I57:AKY,S58:K>Y,S58:K>Y S59:R>S,S60:Y>T,S60:Y>T,S77:N>S,S80:V>I,S81:L>L,S89:V>A S109:Q>Q,S116:S>S IID;;;HHIIIIIII HIIIIIFI;?IIIIIIIHI 11 26 33 30 2 6 24 11 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTGACCTGCACCTTCTCTGGATTCTCACTCAGTACTAGTGCAGTGGGTGTACACTGGATCCGTCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGCCAAATATTACAGCACCAGCCCATCTCTGAAGAGCAGGCTCACCATCACCAAGGACACCTCCAAAAGCCAGGTGATCCTCACAATGACCAACATGGACCCTGCGGACACAGCCACATATTACTGTGCACACAGTGCGGGATGGCTGCCTGATTACTGGGGCCAGGGAACCCTGGTCACCGTCTCTTCAGCCTCCAC
0.02564102564102564 1 IGHV2-5*01 IGHD1-20*01 IGHJ4*01 TGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGG CTHTGMTGIIPIYFDYW S88:G>C,S94:G>C,S98:A>C S119:T>C S165:A>G S185:C>T,S196:A>G,S199:G>A,S215:C>T,D229:AAAACC,S245:T>C,S255:A>G,S264:C>T S291:G>A,S305:A>G S344:A>G,S348:A>G,S362:C>G S29:S>T,S31:S>T,S32:G>G S39:R>R S55:N>D S61:S>S,S65:K>R,S66:S>N,S71:T>T,D76:KNQ,S81:L>L,S85:N>D,S88:P>S S97:A>T,S101:I>M S114:Q>Q,S116:T>A,S120:V>V IIIIIIIIIIII IIII?IIIIII?III?II 10 12 25 35 3 0 4 1 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGACCTGCACCTTCTCTGGGTTCTCACTCACCACTACTGGCGTGGGTGTGGGCTGGATCCGCCAGCCCCCAGGAAAGGCCCTGGAGTGGCTTGCACTCATTTATTGGGATGATGATAAGCGCTACAGTCCATCTCTGAGGAACAGGCTCACCATCACTAAGGACACCTCCAAGGTGGTCCTCACAATGACCGACATGGACTCTGTGGACACAGCCACATATTACTGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTGTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV7-4-1*01 IGHD1/OR15-1a*01 IGHJ4*01 TGTGTGAGACTGGACGGGACCGACTCGGACTACTGG CVRLDGTDSDYW S57:G>A S82:C>T,S90:A>C,S92:C>T,S96:G>T,S98:T>C S101:G>A S153:A>G,S160:A>G,S164:T>C,S169:A>C,S170:C>G S176:G>A,S187:G>A,S200:G>A,S221:C>G,S230:C>T,S242:G>A,S249:T>A,S250:G>A,S251:C>T,S253:G>A,S261:G>T,S266:G>C,S278:G>C S289:C>T S326:A>G S19:V>I S27:T>I,S30:S>R,S30:S>R,S32:A>S,S32:A>S S33:M>I S51:N>D,S53:N>S,S54:T>T,S56:N>T,S56:N>T S58:T>T,S62:G>D,S66:R>R,S73:T>T,S76:S>S,S80:L>L,S83:C>N,S83:C>N,S83:C>N,S84:S>N,S87:A>S,S88:E>D,S92:V>V S96:A>V S108:Q>Q HGGGIIIIIIIII ;IIIIICIIIIIIIIIIIIIIIIIIIH 9 8 14 27 0 6 5 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATCTTCACTCGTTATTCCATAAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCGACACCAGCACCGGGACGCCAACATATGCCCAGGACTTCACAGGACGATTTGTCTTCTCCTTGGACACGTCTGTCAGTACGGCATATCTACAGATCAATAACCTAAAGTCTGACGACACTGCCGTCTATTACTGTGTGAGACTGGACGGGACCGACTCGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 . IGHJ3*01 TGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGG CTRDRHSCAFDVW S90:A>G,S91:G>C,S96:G>T S141:A>C,S148:G>A,S149:G>T S156:A>C,S158:C>A,S167:G>T S176:G>A,S230:C>T,S239:T>C,S240:C>G,S249:T>A,S251:C>T,S258:A>G,S278:G>A S288:G>A,S320:C>G S30:S>A,S30:S>A,S32:A>S S47:M>L,S49:W>Y,S49:W>Y S52:T>P,S52:T>P,S55:G>G S58:T>T,S76:S>S,S79:Y>Y,S80:L>V,S83:C>S,S83:C>S,S86:K>E,S92:V>V S96:A>T,S106:V>V IIIIIIIIIIIIEE II?IIIHHCCIIIIIIIIF 9 -1 -1 23 0 -1 -1 3 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTGCCTATTCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGCTGGGATATATCAACCCAAACACTGGTAACCCAACATATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGTACGGCATACGTGCAGATCAGTAGCCTAGAGGCTGAGGACACTGCCGTATATTACTGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAGCATCCCCG
0.02564102564102564 1 IGHV2-70*01 IGHD3-3*01 IGHJ4*01 TGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGG CARLNPDDFWSGTYYFDHW S77:G>A,S89:C>T,S91:C>G,S102:T>A S108:A>G,S154:T>G S183:A>C,S185:C>T,S200:C>G,S203:G>A,S224:C>T,S226:C>T,S229:A>G,S245:T>C,S275:A>C,S284:T>C,S286:A>T S297:A>T,S314:T>C,S339:T>C S350:A>G S25:G>G,S29:S>S,S30:T>S,S34:C>S S36:S>G,S51:L>R S61:S>R,S61:S>R,S66:T>T,S67:R>R,S74:T>T,S75:S>F,S76:K>R,S81:L>L,S91:T>T,S94:Y>Y,S95:Y>F S99:I>L,S104:F>F,S113:Y>H S116:Q>Q HHHHHIIIIIII IIHIHIIIIIHHIIIIIIII; 12 19 35 40 1 5 10 0 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACTGACCTGCACCTTCTCTGGATTCTCACTCAGTAGTAGTGGAATGAGTGTGGGCTGGATCCGTCAGCCCCCAGGGAAGGCCCTGGAGTGGCTTGCACGCATTGATTGGGATGATGATAAATACTACCGTACATCTCTGAAGACGAGACTCACCATCTCCAAGGACACTTTCAGAAACCAGGTGGTCCTCACAATGACCAACATGGACCCTGTGGACACCGCCACGTACTTCTGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD6-19*01 IGHJ4*01 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG CARGRWLSRPAKDYW S57:G>A,S69:G>A S82:C>T,S83:C>G,S90:A>T,S91:G>C,S92:C>G,S96:G>T S104:T>C,S118:C>T,S121:C>G,S122:T>C S175:C>G,S176:G>T,S179:T>C,S181:C>T,S184:A>C,S188:C>A,S213:T>G,S215:G>C,S230:C>T,S243:C>G,S249:T>A,S257:A>T,S266:G>A,S278:G>A,S284:C>T S335:A>G,S343:T>A S19:V>I,S23:A>T S27:T>M,S27:T>M,S30:S>S,S30:S>S,S30:S>S,S32:A>S S34:N>N,S39:A>V,S40:P>R,S40:P>R S58:T>S,S58:T>S,S59:Y>Y,S60:A>V,S61:Q>P,S62:G>G,S71:L>V,S71:L>V,S76:S>S,S81:Q>E,S83:C>S,S85:L>L,S88:E>E,S92:V>V,S94:Y>Y S111:Q>Q,S114:L>Q GGGIIIIIIIIIIIIIIIII IIII?D?IIIIIHIIIHIIIIIIIIIICI 9 14 21 36 0 10 4 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGACTTCTGGATACATGTTCACTTCGTATTCTATGAACTGGGTGCGACAGGTCCGCGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAAGTTACGTCCCGGGATTCACAGGACGGTTTGTCTTCTCCGTCGACACCTCTGTCAGTACGGCATATCTGGAGATCAGCAGCCTTAAGGCTGAAGACACTGCCGTATATTATTGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV1-69*01 IGHD1-7*01 IGHJ2*01 TGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGG CARDLGNYAGYSDLW S67:A>G S79:G>A,S82:C>T,S83:C>T,S89:C>A,S91:G>C,S97:C>G S103:G>C,S104:C>T,S131:G>C S159:A>G,S160:T>C,S167:T>A,S171:G>T,S172:C>T,S173:A>C S174:A>C,S188:G>A,S191:C>T,S201:G>C,S206:G>C,S219:G>A,S221:A>C,S226:C>T,S228:A>G,S229:G>C,S230:C>T,S235:C>T,S252:A>G,S259:G>C,S278:G>A,S284:C>T S291:A>C,S319:T>C S22:K>R S26:G>D,S27:T>I,S27:T>I,S29:S>R,S30:S>T,S32:A>G S34:S>T,S34:S>T,S43:G>G S53:I>A,S53:I>A,S55:G>G,S57:A>F,S57:A>F,S57:A>F S58:N>H,S62:K>K,S63:F>F,S67:V>L,S68:T>T,S73:E>N,S73:E>N,S75:T>M,S76:S>A,S76:S>A,S76:S>A,S78:A>V,S84:S>G,S86:R>T,S92:V>V,S94:Y>Y S97:R>R,S106:F>S IIIIHIII@@ ?D==??IIIIIIIIIIIH;IIIIIIIIIIIIIII 11 16 23 28 0 9 1 5 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAGGGCTTCTGGAGACATTTTCAGAACCTATGGTATCACTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATGGGAGGGATCATCCCTGCCTTTGGAACATTCCACTACGCACAGAAATTTCAGGGCAGACTCACCATTACCGCGGACAACTCCATGGCTACAGTCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTATATTATTGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV3-30-3*01 IGHD1-14*01 IGHJ6*01 TGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGG CAKDREPTYIHYMDVW S91:G>C,S92:C>T,S93:T>C,S96:G>T,S97:C>G S99:A>G,S101:G>A S152:A>G,S156:T>A,S157:A>T,S169:A>C,S171:A>G,S172:A>G S212:C>T,S232:C>T,S240:C>T,S259:G>C,S261:G>A,S266:G>A,S278:G>A S335:G>C S30:S>T,S30:S>T,S31:Y>H,S32:A>C,S32:A>C S33:M>V,S33:M>V S50:I>M,S52:Y>I,S52:Y>I,S56:N>T,S57:K>G,S57:K>G S70:S>S,S77:T>M,S80:L>L,S86:R>T,S87:A>T,S88:E>E,S92:V>V S111:G>G HHICHD?IIIIIIIIHHHHHH IIIIIIIIIIICCIIHHCIDH 7 14 21 35 2 9 1 19 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACTCATTGTGTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATGTCAATTGATGGAAGCACTGGATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCTAGAGACAATTCCAAGAACATGCTGTATTTGCAAATGAACAGCCTGACAACTGAAGACACGGCTGTATATTACTGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV4-31*01 IGHD2/OR15-2a*01 IGHJ3*01 TGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGG CTRILGRDDAFDMW S66:A>T S88:G>C,S89:C>T S108:A>T,S109:G>C,S114:A>G,S131:G>A,S152:G>T,S155:C>T S156:A>G,S160:A>T,S170:G>T,S172:G>C S177:T>A,S189:T>G,S202:T>G,S203:A>C,S206:T>C,S207:A>T,S212:A>T,S218:A>T,S247:A>C,S248:G>C,S253:G>C,S258:G>C,S260:G>A,S279:G>A,S281:G>T S291:G>A,S324:G>A,S326:C>G S335:A>C S22:T>S S29:S>T,S29:S>T S36:S>S,S36:S>S,S38:I>V,S43:G>G,S50:G>G,S51:Y>Y S52:I>V,S53:Y>F,S56:G>G,S57:S>T S59:Y>N,S63:S>A,S67:L>R,S67:L>R,S68:V>V,S69:T>S,S70:I>I,S72:V>V,S82:K>T,S82:K>T,S84:S>T,S86:V>L,S86:V>L,S93:V>I,S93:V>I S97:A>T,S108:V>M,S108:V>M S111:Q>H BB@H>HEI IIIIIHHIII>IIICIIII;IHHIIIE?IEE< 9 6 15 23 2 0 22 0 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCTCTGTCTCTGGTGGCTCCATCACTAGTGGTGGTTACTACTGGTCCTGGGTCCGCCAGCACCCAGGAAAGGGCCTGGAGTGGATTGGTTATGTCTTTTACAGTGGTACCACCAACTACAACCCGGCCCTCAAGAGTCGCGTCTCCATTTCAGTTGACACGTCTAAGAACCAGTTCTCCCTGACCCTGACCTCTCTAACTGCCGCGGACACGGCCATTTATTACTGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGGGGCCACGGGACAATGGTCACCGTCTCTTCAGCATCCCCG
0.02564102564102564 1 IGHV1-69*01 IGHD6-13*01 IGHJ6*01 TGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGG CARGSQAADYYYGMDVW S83:C>G,S90:A>C,S91:G>A S162:T>G S176:C>G,S187:A>G,S188:G>A,S197:C>T,S201:G>C,S206:G>T,S210:A>T,S216:G>C,S230:C>A,S233:A>C,S237:T>G,S242:G>C,S259:G>C,S266:G>C,S278:G>T S338:G>C,S350:G>A S27:T>T,S30:S>H,S30:S>H S54:F>V S58:N>K,S62:K>R,S62:K>R,S65:G>G,S67:V>L,S68:T>T,S70:T>S,S72:D>H,S76:S>R,S77:T>T,S79:Y>D,S80:M>I,S86:R>T,S88:E>D,S92:V>V S112:G>G,S116:T>T HHIHHHIHH IIIIIIIIII?II?;IIIHHH 10 17 25 27 1 9 4 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACGTTCAGCCACTATGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCGTTGGTACAGCAAAGTACGCACAGAGATTCCAGGGTAGACTCACTATTTCCGCGCACGAATCCACGAGAACCGCCGACATCGAGCTGAGCAGCCTGACATCTGACGACACGGCCGTTTATTACTGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCGCGGCATCCCCG
0.02564102564102564 1 IGHV7-4-1*01 IGHD3-9*01 IGHJ6*01 TGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGG CAREGYYFDTTGSPRSHGLDVW S59:T>C S76:G>C,S82:C>G,S83:C>T,S90:A>G,S91:G>A,S92:C>G S99:A>C,S103:A>C,S128:A>G,S135:G>C S159:A>G,S160:A>G,S161:C>A,S168:A>G S187:G>C,S200:G>T,S212:C>G,S214:T>C,S236:A>T,S245:G>C,S249:T>A,S252:A>G,S254:C>T,S261:G>C,S278:G>T,S284:C>T S339:A>C S353:G>C,S363:A>G S19:V>V S25:G>A,S27:T>S,S27:T>S,S30:S>E,S30:S>E,S30:S>E S33:M>L,S34:N>T,S42:Q>Q,S45:E>Q S53:N>G,S53:N>G,S53:N>G,S56:N>D S62:G>A,S66:R>R,S70:S>S,S71:L>S,S78:A>A,S81:Q>H,S83:C>S,S84:S>G,S84:S>G,S87:A>P,S92:V>V,S94:Y>Y S113:M>L S117:G>G,S121:T>A IICCCCIIIIIIIIIIIIIIIIIIIIIIIIII IIIHIIIII?IIIICI@IIIIIIIIHIIII 9 18 26 49 0 9 14 15 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACAGTTTCACTGAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTCAGTGGATGGGATGGATCAACACCGGAACTGGGGACCCAACGTATGCCCAGGCCTTCACAGGACGTTTTGTCTTCTCGTCGGACACCTCTGTCAGCACGGCTTATCTGCACATCAGCGGTCTAAAGCCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD1-20*01 IGHJ4*01 TGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGG CGRESLGHWIDYW S45:G>A,S51:G>T,S57:G>C,S69:G>A,S71:T>C S81:A>T,S91:G>A S176:G>A,S187:G>C,S213:T>C,S230:C>T,S234:G>A,S243:C>G,S249:T>A,S250:G>A,S253:G>A,S266:G>A,S271:C>G,S276:G>A,S278:G>A S289:C>G S329:A>G S15:A>T,S17:V>L,S19:V>L,S23:A>T,S23:A>T S27:T>S,S30:S>N S58:T>T,S62:G>A,S71:L>L,S76:S>S,S78:A>T,S81:Q>E,S83:C>N,S83:C>N,S84:S>N,S88:E>E,S90:T>S,S92:V>I,S92:V>I S96:A>G S109:Q>Q DHIIIIIIIIIII IIIIIII?I;IIIIIIIIIIIH 9 22 28 28 0 6 5 6 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCATTGAAGCTTTCCTGCAAGACCTCTGGATACTCCTTCACTAACTATGCTATGAATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGGTTTGTCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACCTAAAGGCTGAAGACAGTGCCATATATTACTGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV4-31*01 IGHD4-17*01 IGHJ1*01 TGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGG CATYGRSRETEYFQYW S67:C>G S84:A>C,S88:G>C,S94:G>T,S97:G>A S114:A>C,S155:C>T S167:T>C,S174:A>G S178:A>G,S179:C>G,S194:C>G,S202:T>G,S204:G>A,S207:A>T,S210:A>T,S218:A>C,S236:G>A,S247:A>T,S248:G>A,S249:C>G,S253:G>A,S281:G>C,S287:C>T S330:C>T S22:T>S S28:I>L,S29:S>T,S31:G>V,S32:G>D S38:I>L,S51:Y>Y S55:S>S,S58:T>A S59:Y>W,S59:Y>W,S64:L>L,S67:L>R,S68:V>I,S69:T>S,S70:I>L,S72:V>V,S78:Q>Q,S82:K>I,S82:K>I,S83:L>V,S84:S>N,S93:V>V,S95:Y>Y S110:H>Y IIIIIIIIIIIIII DCIIIHIDIIIH??GIIIIIIIIII 7 8 15 28 4 3 6 1 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCCTCACCAGTGTTGATTACTACTGGAGCTGGCTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTATATCTATTACAGCGGGAGCGCCTGGTACAACCCGTCCCTGAAGAGTCGAATTTCCTTATCAGTCGACACGTCTAAGAACCAATTCTCCCTGATAGTGAACTCTGTGACTGCCGCGGACACGGCCGTCTATTATTGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV3-33*01 IGHD2/OR15-2a*01 IGHJ6*01 TGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGG CAREDDTSTWYGRLRYYYAMDVW S84:T>A S171:A>G S220:A>C,S224:C>A,S225:A>C,S234:C>G,S246:A>C,S251:C>T,S278:G>T,S284:C>T S356:G>C,S377:C>G S28:F>I S57:K>E S73:N>T,S74:S>S,S75:K>Q,S78:L>V,S82:M>L,S83:N>N,S92:V>V,S94:Y>Y S118:G>G,S125:V>V IIIIIIIIIIIIIIIIIIIIIIHHHIIIIICC?IIIII IIIIIIIIGIII 11 44 52 57 0 14 9 20 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCATCAGTAGCTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAGTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCACAGAACACGGTGTATCTGCAACTGAATAGCCTGAGAGCCGAGGACACGGCTGTTTATTATTGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV6-1*01 IGHD1-1*01 IGHJ5*01 TGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGG CARGEGTTATYNFDPW S97:G>C,S100:C>A,S101:T>A S160:A>T,S161:C>A,S182:T>C S183:G>A,S206:T>C,S222:C>G,S229:C>T,S232:C>A,S259:A>G,S260:C>G,S275:G>C,S279:A>G S336:T>C S347:A>G S32:S>T,S33:A>E,S33:A>E S53:Y>L,S53:Y>L,S60:N>N S61:D>N,S68:S>S,S74:P>A,S76:T>I,S77:S>Y,S86:N>R,S86:N>R,S91:E>D,S93:T>A S112:S>P S115:Q>Q BBBIIHHHIIIIIIIIIIII EEDIIIIEIIIIIIICH 10 30 37 36 1 2 8 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTAGCAACACTGAAGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATTATACAGGTCCAAGTGGTATAACAATTATGCAGTATCTGTGAAAAGCCGAATAACCATCAACGCAGACATATACAAGAACCAGTTCTCCCTGCAGCTGAGGTCTGTGACTCCCGACGACGCGGCTGTGTATTACTGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV6-1*01 IGHD5-12*01 IGHJ4*01 TGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGG CAGDRYNSGGGDYW S67:C>T S77:G>C,S92:C>T,S93:A>G,S98:T>A,S99:G>A,S100:C>T S108:A>C,S114:A>G,S122:G>A S159:T>A,S160:A>T,S161:C>G,S162:T>C,S171:A>G S191:A>C,S192:G>T,S198:G>A,S203:A>G,S212:A>T,S214:C>T,S215:C>T,S218:C>T,S220:A>G,S221:C>G,S230:A>G,S235:A>G,S243:T>C,S247:C>T,S248:C>T,S254:G>A,S255:C>T,S259:A>C,S285:G>A,S287:G>T,S293:C>T S300:A>G S341:A>G,S346:C>T S22:A>V S25:G>G,S30:S>S,S31:N>D,S32:S>R,S33:A>I,S33:A>I S36:N>H,S38:I>V,S40:Q>Q S53:Y>M,S53:Y>M,S53:Y>M,S54:Y>H,S57:K>E S63:A>A,S64:V>L,S66:V>M,S67:K>K,S70:I>I,S71:T>I,S71:T>I,S72:I>I,S73:N>R,S73:N>R,S76:T>T,S78:K>R,S81:F>L,S82:S>F,S82:S>F,S84:Q>Q,S85:L>L,S86:N>T,S95:V>I,S95:V>I,S97:Y>Y S100:R>G S113:Q>Q,S115:T>I IIIIIIII==HIII IIIIHHIIHIIIIIIIIIIHHHIIIHIIHHICIIHIIHI 11 19 27 33 0 6 9 8 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTGTCATCTCCGGCGACAGTGTCTCTAGTGACAGAATTGCTTGGCACTGGGTCAGGCAATCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACAATGCACAGGTCCGAGTGGTATAATGATTATGCCTTATCTATGAAGAGTCGAATTATTATTAGGCCAGACACGTCCAGGAACCAGCTCTTTCTGCAATTGACCTCTGTGACTCCCGAGGACACGGCTATTTATTATTGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD1-26*01 IGHJ6*01 TGTGCGAGAGATAGTGGGGGTATGGACGTCTGG CARDSGGMDVW S96:G>T S102:A>C S152:C>T,S156:A>T,S157:C>T,S169:A>C S249:T>A S32:A>S S34:N>H S50:I>I,S52:T>F,S52:T>F,S56:N>T S83:C>S I IIIIICI 9 10 18 18 0 3 9 17 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCTTCACTAGCTATTCTATGCATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATTAACTTCAACACTGGGACCCCAACGTATGCCCAGGGCTTCACAGGACGGTTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGGCTGAGGACACTGCCGTGTATTACTGTGCGAGAGATAGTGGGGGTATGGACGTCTGGGGGC
0.02564102564102564 1 IGHV7-4-1*01 IGHD6-13*01 IGHJ1*01 TGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGG CSTLQPRIAAPEFW S57:G>A S82:C>T,S83:C>T,S91:G>A,S92:C>A,S96:G>T,S98:T>A S103:A>G,S148:G>A,S149:G>C S160:A>T,S167:G>T S176:G>T,S179:T>C,S187:G>C,S209:C>T,S225:G>A,S249:T>A,S261:G>A,S266:G>C,S274:C>G,S276:G>A,S284:C>T S288:G>T S335:C>A,S336:A>G,S345:A>G S19:V>I S27:T>I,S27:T>I,S30:S>K,S30:S>K,S32:A>S,S32:A>S S34:N>S,S49:W>Y,S49:W>Y S53:N>I,S55:G>G S58:T>T,S59:Y>Y,S62:G>A,S69:F>F,S75:V>I,S83:C>S,S87:A>T,S88:E>D,S91:A>G,S92:V>M,S94:Y>Y S96:A>S S111:G>G,S112:T>A,S115:T>A HHHIIIIIIIIIIIIIIIIIIIIII IBBHHCIIIIIIIII:IIIIIIIIHII 7 24 30 38 2 7 8 17 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGCCTGGGGCCTCAGTGAAGATTTCCTGCAAGGCTTCTGGATACATTTTCACTAAATATTCAATGAGTTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATACATCAACACCATCACTGGTAACCCAACTTACGCCCAGGCCTTCACAGGACGGTTTGTCTTTTCCTTGGACACCTCTATCAGCACGGCATATCTGCAGATCAGCAGCCTAAAGACTGACGACACTGGCATGTATTATTGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGGGGCCAGGGAGCCCTGGTCGCCGTCTCCTCGGCCTCCAC
0.02564102564102564 1 IGHV4-59*01 IGHD3-3*01 IGHJ1*01 TGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGG CARGSYNYWSDYCSHLDYW S66:A>T,S68:T>C,S69:G>A S90:A>C,S91:G>C S110:C>T,S117:C>T,S126:A>C,S137:G>A S165:A>C,S167:C>T S171:A>G,S172:A>C,S173:C>T,S178:A>G,S206:A>G,S210:G>C,S230:G>A,S242:G>C,S246:A>G S313:G>A S22:T>S,S22:T>S,S23:V>I S30:S>P,S30:S>P S36:I>I,S39:P>S,S42:K>Q,S45:E>E S55:S>R,S55:S>R S57:N>A,S57:N>A,S57:N>A,S59:N>S,S68:I>M,S70:V>L,S76:Q>Q,S80:K>N,S82:S>G S104:G>D IIIIIIIIIIIIIHHIHHHIIIIIIIII IIIII?DIIIIIIIIIIIIHI 10 23 37 52 1 12 5 16 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCTCCATCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATTCGGCAGTCCCCAGGGCAGGGACTGGAATGGATTGGGTATATCTATTACAGTGGGCGTACCGCTTACAGCCCCTCCCTCAAGAGTCGAGTCACCATGTCACTAGACACGTCCAAGAACCAATTCTCCCTGAACCTGGGCTCTGTGACCGCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV6-1*01 IGHD1/OR15-1a*01 IGHJ1*01 TGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGG CARGREQDLPSADFNQW S90:A>C,S94:A>G,S97:G>T,S100:C>T,S102:G>A,S104:T>C S122:G>A,S128:A>G S157:C>T,S158:A>C,S172:A>G,S178:A>T,S180:A>T S185:T>G,S189:G>T,S194:A>G,S198:G>C,S200:G>A,S201:A>C,S204:A>G,S212:A>G,S213:A>C,S214:C>G,S216:A>T,S219:A>T,S223:C>A,S242:G>A,S243:T>C,S254:G>A,S255:C>T,S260:C>T,S262:C>T,S268:C>G,S275:G>A,S287:G>C,S292:A>T S348:C>A,S353:C>A,S355:C>T,S365:C>T S30:S>R,S31:N>S,S32:S>I,S33:A>V,S34:A>T,S34:A>T S40:Q>Q,S42:P>P S52:T>I,S52:T>I,S57:K>R,S59:Y>F,S60:N>Y S61:D>E,S63:A>S,S64:V>V,S66:V>L,S66:V>L,S67:K>Q,S68:S>G,S70:I>M,S71:T>R,S71:T>R,S72:I>F,S73:N>Y,S74:P>Q,S80:Q>Q,S81:F>L,S84:Q>Q,S85:L>L,S86:N>N,S87:S>F,S89:T>S,S91:E>E,S95:V>V,S97:Y>F S116:Q>K,S117:G>G,S118:T>I,S121:T>T IIIIIIIIIIIIIIIIIIIIIIICCCIIIIIA IIIIIIIIIII;;IIIIIIIIIIIIIIIIICCIIIHIHII 10 15 21 48 1 10 1 18 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTGCCATCTCCGGGGACAGTGTCTCTCGCAGCATTGTTACCTGGAACTGGATCAGGCAATCCCCGTCGAGAGGCCTTGAGTGGCTGGGAAGGATCTACTACAGGTCCAGGTGGTTTTATGAGTATTCAGTGTCTCTACAAGGTCGAATGCGCTTCTACCAAGACACATCCAAGAACCAACTCTCCCTGCAATTGAATTTTGTGAGTCCCGAAGACACGGCTGTCTATTTCTGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGGGGCAAGGGAATCCTGGTCACTGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD1-1*01 IGHJ6*01 TGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGG CAREGYYIDTTGSPRSHGLDVW S59:T>C S76:G>C,S82:C>T,S83:C>T,S89:T>A,S91:G>A,S92:C>G S99:A>C,S103:A>C,S128:A>G S159:A>G,S160:A>G,S168:A>G S187:G>C,S191:C>T,S200:G>T,S212:C>T,S214:T>C,S245:G>C,S249:T>A,S252:A>G,S254:C>T,S278:G>T,S284:C>T S339:A>T S353:G>C,S363:A>G S19:V>V S25:G>A,S27:T>I,S27:T>I,S29:T>T,S30:S>K,S30:S>K S33:M>L,S34:N>T,S42:Q>Q S53:N>G,S53:N>G,S56:N>D S62:G>A,S63:F>F,S66:R>R,S70:S>S,S71:L>S,S81:Q>H,S83:C>S,S84:S>G,S84:S>G,S92:V>V,S94:Y>Y S113:M>L S117:G>G,S121:T>A IIHHHIIIIIIIIIIIIIIIIIIIIIIIIII BI@@GGIIH;IIBIHCIHIIIIHIHII 9 26 35 49 0 2 6 15 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGCATACATTTTCACAAAGTATGCTCTGACTTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACACCGGCACTGGGGACCCAACGTATGCCCAGGCCTTTACAGGACGTTTTGTCTTCTCTTCGGACACCTCTGTCAGCACGGCATATCTGCACATCAGCGGTCTAAAGGCTGAGGACACTGCCGTTTATTATTGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAGCCTCCAC
0.02564102564102564 1 IGHV4-34*01 IGHD3-9*01 IGHJ4*01 TGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGG CARARGDGYYNDRLRFDLW S94:A>T,S96:T>G,S97:A>C S103:G>C,S113:C>T,S117:C>T,S127:A>T,S146:G>A,S149:A>T S150:A>C S194:T>G,S202:C>G,S204:A>T,S206:A>T,S207:T>A,S216:A>T,S224:G>A,S226:A>G,S227:C>T,S230:G>C,S241:A>G,S247:G>C,S248:C>T,S275:G>C S284:T>C,S334:A>T,S335:C>G S344:A>G S31:Y>F,S32:Y>A,S32:Y>A S34:S>T,S37:R>R,S39:P>S,S42:K>M,S48:G>G,S49:E>D S50:I>L S64:S>R,S67:T>S,S68:I>F,S68:I>F,S69:S>T,S72:T>S,S74:K>K,S75:N>S,S75:N>S,S76:Q>H,S80:K>R,S82:S>T,S82:S>T,S91:V>V S94:C>C,S111:Y>L,S111:Y>L S114:Q>Q HHCCCIIIIIIIIIIIIIIIIIIII HIIHIBBIIIIICCIICIIIIIIIIIIH 8 20 32 45 3 18 1 5 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCAGTGGTTTCGCCTGGACCTGGATCCGTCAGTCCCCAGGGATGGGGCTGGAGTGGATTGGAGATCTCAATCATAGTGGAAGCACCAACTACAACCCGTCCCTCAAGAGGCGAGTCAGCTTTACAGTAGACTCGTCCAAAAGTCACTTCTCCCTGAGGCTGACTTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV4-59*01 IGHD3-10*01 IGHJ6*01 TGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGG CASLKRGGDFYYALDVW S82:C>T,S86:C>G,S89:T>G,S93:T>G S104:C>T,S147:T>C S158:C>T,S160:G>A,S165:A>G,S166:G>T S178:A>G,S197:A>G,S201:A>G,S211:T>C,S243:C>T,S246:A>C,S251:T>C,S253:T>C,S275:G>A S319:G>C,S321:A>T S335:G>C S27:S>F,S28:I>M,S29:S>R,S31:Y>D S34:S>S,S49:Y>H S52:Y>Y,S53:S>N,S55:S>V,S55:S>V S59:N>S,S65:R>R,S67:T>A,S70:V>A,S81:L>L,S82:S>R,S83:S>S,S84:V>A,S91:V>V S106:G>A,S107:M>L S111:G>G IIIIIIIIIIIIIII HIIIIIIICIIHIIIIIIIIGI 8 16 22 29 3 15 10 10 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTTCATGAGGAGTGACTACTGGAGTTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGCATATCTATTATAATGGGGTCACCAACTACAGCCCCTCCCTCAAGAGTCGGGTCGCCATATCAGCAGACACGTCCAAGAACCAGTTCTCCCTGAAGTTGCGCTCCGCGACCGCTGCGGACACGGCCGTATATTACTGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV4-59*01 IGHD2-2*01 IGHJ4*01 TGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGG CARGYSYDLYGNKGGFDYW S67:C>G S77:T>C,S81:T>G,S82:C>G,S84:A>T,S85:T>C,S87:A>G,S89:T>C,S90:A>C,S91:G>A,S92:T>C,S95:C>T,S98:C>T S103:G>C,S104:C>T,S117:C>T,S129:G>A,S130:G>C,S137:G>A,S141:A>C,S146:G>C,S148:A>T,S149:T>C S156:T>G,S158:C>T,S159:A>G,S161:T>G,S163:G>C,S164:G>C,S166:G>A,S167:C>T,S168:A>T,S169:C>T S173:C>G,S186:C>G,S188:C>G,S193:G>A,S206:A>G,S216:A>C,S225:A>G,S226:A>G,S231:T>A,S233:C>G,S243:C>G,S247:G>C,S256:C>G,S257:C>T,S259:C>T,S275:G>C S335:C>T S344:A>G,S349:C>T,S352:T>G S22:T>S S25:G>G,S27:S>G,S27:S>G,S28:I>S,S28:I>S,S29:S>G,S29:S>G,S30:S>H,S30:S>H,S30:S>H,S31:Y>Y,S32:Y>Y S34:S>T,S34:S>T,S39:P>S,S43:G>T,S43:G>T,S45:E>E,S47:I>L,S48:G>G,S49:Y>F,S49:Y>F S52:Y>D,S52:Y>D,S53:S>G,S53:S>G,S54:G>A,S54:G>A,S55:S>N,S55:S>N,S56:T>F,S56:T>F S57:N>K,S62:L>V,S62:L>V,S64:S>N,S68:I>M,S72:T>P,S75:N>G,S75:N>G,S77:F>M,S77:F>M,S81:L>V,S82:S>T,S85:T>S,S85:T>S,S86:A>V,S91:V>V S111:Y>Y S114:Q>Q,S116:T>I,S117:L>R BIIIIIIIIIIIIIIIIIIIICCCIIIIIC IIIIIIIIIII=III?EIEI???IIAAIIIDHHHIHII?;;IIHHIIIIIHIH 8 9 16 45 3 1 23 5 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGTGTCTCTGGCGGCGGCTCCGGCCACTATTATTGGACTTGGATCCGGCAGTCCCCAGGGAAGACACTGGAATGGCTTGGCTTCATCTATGATGGGGCCAATTTCAAGTACAACCCCTCCGTGAAGAATCGAGTCACCATGTCAGTAGACCCGTCCAAGGGCCAGATGTCCCTGAAGGTGACCTCTGTGAGTGTTGCGGACACGGCCGTCTATTACTGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGGGGCCAGGGAATCCGGGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV6-1*01 IGHD3-10*01 IGHJ4*01 TGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGG CARDKSRDDYYDHW S65:T>C,S67:C>T S77:G>A,S82:G>A,S83:T>C,S93:A>G,S97:G>C S192:G>A,S194:A>T,S198:G>A,S201:A>G,S214:C>T,S215:C>T,S221:C>G,S242:G>C,S255:C>T,S260:C>G,S264:G>C,S287:G>C,S293:C>T S325:T>A,S330:T>C S341:A>G S21:C>C,S22:A>V S25:G>G,S27:S>N,S27:S>N,S31:N>D,S32:S>T S64:V>I,S64:V>I,S66:V>M,S67:K>E,S71:T>I,S71:T>I,S73:N>K,S80:Q>H,S85:L>L,S86:N>K,S88:V>L,S95:V>V,S97:Y>Y S108:F>Y,S110:Y>H S113:Q>Q IIIIIIIII IIHIIIIHHIIIIIIHIIIIIIH 11 17 22 25 0 13 13 0 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGTCATCTCCGGAGACAACGTCTCTAGCGACACTGCTGCTTGGAACTGGATCAGGCAGTCCCCATCGAGAGGCCTTGAGTGGCTGGGAAGGACATACTACAGGTCCAAGTGGTATAATGATTATGCAATTTCTATGGAAAGTCGAATAATTATCAAGCCAGACACATCCAAGAACCACTTCTCCCTGCAGTTGAAGTCTCTGACTCCCGAGGACACGGCTGTCTATTATTGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCGCAACCTCCAC
0.02564102564102564 1 IGHV7-4-1*01 IGHD1-1*01 IGHJ4*01 TGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGG CARDLPVGGTTPSDYW S55:A>G,S66:A>G,S74:T>C S89:T>C,S90:A>T,S91:G>C,S96:G>A S101:G>A,S102:A>C S170:C>A,S173:A>G S200:G>A,S249:T>A,S250:G>A,S253:G>A,S255:C>G,S258:A>C,S272:T>C,S276:G>A,S278:G>T S338:A>G,S347:G>A S18:K>R,S22:K>E,S24:S>S S29:T>T,S30:S>S,S30:S>S,S32:A>T S33:M>I,S34:N>H S56:N>K,S57:P>P S66:R>R,S83:C>N,S83:C>N,S84:S>N,S85:L>V,S86:K>Q,S90:T>T,S92:V>I,S92:V>I S112:Q>Q,S115:L>L IIIIIIIIIIIIFFD;??HI HIIIIIIIIHIIIH=?I?HC?I 9 23 32 38 0 8 0 7 13 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGGCCTCAGTGAGGGTTTCCTGCGAGGCTTCCGGATACACCTTCACCTCCTATACTATACATTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACACCAACACTGGGAAACCGACGTATGCCCAGGGCTTCACAGGACGATTTGTCTTCTCCTTGGACACCTCTGTCAGCACGGCATATCTGCAGATCAACAACGTACAGGCTGAGGACACCGCCATTTATTACTGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCCG
0.02564102564102564 1 IGHV7-4-1*01 IGHD1-20*01 IGHJ4*01 TGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGG CGRESLGHWIDYW S45:G>A,S69:G>A,S71:T>C S81:A>T,S88:C>G,S91:G>A S113:A>G,S146:A>G S158:C>T,S161:C>T S176:G>A,S187:G>C,S200:G>A,S204:G>A,S213:T>C,S230:C>T,S234:G>A,S243:C>G,S249:T>A,S250:G>A,S253:G>A,S255:C>T,S257:A>G,S266:G>A,S269:C>T,S276:G>A,S278:G>A S289:C>G,S290:G>A,S320:C>T S329:A>G,S342:A>G S15:A>T,S23:A>T,S23:A>T S27:T>S,S29:T>S,S30:S>N S37:R>R,S48:G>G S52:T>T,S53:N>N S58:T>T,S62:G>A,S66:R>R,S68:V>I,S71:L>L,S76:S>S,S78:A>T,S81:Q>E,S83:C>N,S83:C>N,S84:S>N,S85:L>L,S85:L>L,S88:E>E,S89:D>D,S92:V>I,S92:V>I S96:A>G,S96:A>G,S106:Y>Y S109:Q>Q,S114:T>A HHIIIIIIIIIII IIIIIIIAIIIIHDCIIIIIIIHHIIIII=?D 9 22 28 28 0 6 5 6 -1 -1 -1 -1 true true true true true NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGAAGCCTGGGACCTCAGTGAAGGTTTCCTGCAAGACCTCTGGATACTCCTTCAGTAACTATGCTATGAATTGGGTGCGGCAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAACACTAATACTGGGAACCCAACATATGCCCAGGCCTTCACAGGACGATTTATCTTCTCCCTGGACACCTCTGTCAGTACGACATATCTGGAGATCAACAACTTGAAGGCTGAAGATACTGCCATATATTACTGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGGGGCCAGGGAACCCTGGTCGCCGTCTCCTCAGCCTCCAC
Raw
SRR765688_vdj.txt
count freq cdr3nt cdr3aa v d j VEnd DStart DEnd JStart
2 0.05128205128205128 TGTGCGAGAGATTCCTTGCACGACTACGGTATGGACGTCTGG CARDSLHDYGMDVW IGHV1-46 IGHD1-1 IGHJ6 11 19 24 22
2 0.05128205128205128 TGTGCGAGAGATCGAGGTAGTACCAGGTGGCTCGAACCCATTGACTACTGG CARDRGSTRWLEPIDYW IGHV1-18 IGHD2-2 IGHJ4 11 16 26 40
2 0.05128205128205128 TGTGCGAGAGGGAGGTGGCTGAGTCGGCCGGCCAAGGACTACTGG CARGRWLSRPAKDYW IGHV7-4-1 IGHD6-19 IGHJ4 9 14 21 36
1 0.02564102564102564 TGTGCGAGAGATCCGAACGCGATAGTGGTGGAAGACTGGTTCGACCCCTGG CARDPNAIVVEDWFDPW IGHV7-4-1 IGHD2-15 IGHJ5 9 22 31 34
1 0.02564102564102564 TGTGCGAGACTCGACGGCAGCGATAGGGACTTCTGG CARLDGSDRDFW IGHV7-4-1 IGHD6-25 IGHJ4 9 16 22 27
1 0.02564102564102564 TGTGCGAGAGATCTAAATTATATACACTTCGACCCCTGG CARDLNYIHFDPW IGHV7-4-1 IGHD3-10 IGHJ5 9 16 22 27
1 0.02564102564102564 TGTGCGAGACACGATAGCAGCAGCTGGTACGTACAGGTTGACTACTGG CARHDSSSWYVQVDYW IGHV5-10-1 IGHD6-13 IGHJ4 9 13 30 37
1 0.02564102564102564 TGTGCGAGACAATGGGATGATACTGGGAACTACTACGCCCGGGCTCACTTTGACTCTTGG CARQWDDTGNYYARAHFDSW IGHV4-39 IGHD1-7 IGHJ4 11 25 33 46
1 0.02564102564102564 TGTGTGAGGAATATAAGGCGCTCCGATAATACCGCTTATTATGCCGAGTACTGG CVRNIRRSDNTAYYAEYW IGHV5-51 IGHD3-10 IGHJ4 8 35 42 48
1 0.02564102564102564 TGTGCGAGACATTCCGGGCCTAACAGTGTCTTGTTCTGGTTCGACCCCTGG CARHSGPNSVLFWFDPW IGHV4-59 IGHD5/OR15-5a IGHJ5 9 24 31 35
1 0.02564102564102564 TGTGCACACAGTGCGGGATGGCTGCCTGATTACTGG CAHSAGWLPDYW IGHV2-5 IGHD3-16 IGHJ4 11 26 33 30
1 0.02564102564102564 TGTACACACACTGGTATGACTGGAATTATTCCGATCTACTTTGACTACTGG CTHTGMTGIIPIYFDYW IGHV2-5 IGHD1-20 IGHJ4 10 12 25 35
1 0.02564102564102564 TGTGTGAGACTGGACGGGACCGACTCGGACTACTGG CVRLDGTDSDYW IGHV7-4-1 IGHD1/OR15-1a IGHJ4 9 8 14 27
1 0.02564102564102564 TGTACGAGAGACAGACATTCATGTGCTTTTGATGTGTGG CTRDRHSCAFDVW IGHV7-4-1 . IGHJ3 9 -1 -1 23
1 0.02564102564102564 TGTGCACGGTTAAACCCTGACGATTTCTGGAGTGGCACCTACTACTTTGACCACTGG CARLNPDDFWSGTYYFDHW IGHV2-70 IGHD3-3 IGHJ4 12 19 35 40
1 0.02564102564102564 TGTGCGCGAGATCTTGGGAACTATGCGGGGTACTCCGATCTCTGG CARDLGNYAGYSDLW IGHV1-69 IGHD1-7 IGHJ2 11 16 23 28
1 0.02564102564102564 TGTGCGAAAGACAGGGAACCAACTTATATTCACTATATGGACGTCTGG CAKDREPTYIHYMDVW IGHV3-30-3 IGHD1-14 IGHJ6 7 14 21 35
1 0.02564102564102564 TGTACGAGAATATTGGGGAGAGATGATGCTTTTGATATGTGG CTRILGRDDAFDMW IGHV4-31 IGHD2/OR15-2a IGHJ3 9 6 15 23
1 0.02564102564102564 TGTGCGAGAGGGTCCCAAGCAGCTGATTACTACTACGGTATGGACGTCTGG CARGSQAADYYYGMDVW IGHV1-69 IGHD6-13 IGHJ6 10 17 25 27
1 0.02564102564102564 TGTGCGAGAGAGGGGTATTATTTTGATACCACTGGTTCTCCGAGGTCACACGGTCTGGACGTCTGG CAREGYYFDTTGSPRSHGLDVW IGHV7-4-1 IGHD3-9 IGHJ6 9 18 26 49
1 0.02564102564102564 TGTGGGAGAGAGAGTCTCGGCCACTGGATTGACTACTGG CGRESLGHWIDYW IGHV7-4-1 IGHD1-20 IGHJ4 9 22 28 28
1 0.02564102564102564 TGTGCGACCTACGGTCGCAGTCGCGAGACTGAATACTTCCAGTACTGG CATYGRSRETEYFQYW IGHV4-31 IGHD4-17 IGHJ1 7 8 15 28
1 0.02564102564102564 TGTGCGAGAGAGGATGATACCAGTACCTGGTACGGGCGACTCCGGTACTACTATGCCATGGACGTCTGG CAREDDTSTWYGRLRYYYAMDVW IGHV3-33 IGHD2/OR15-2a IGHJ6 11 44 52 57
1 0.02564102564102564 TGTGCAAGAGGGGAAGGGACAACTGCAACTTACAACTTCGACCCCTGG CARGEGTTATYNFDPW IGHV6-1 IGHD1-1 IGHJ5 10 30 37 36
1 0.02564102564102564 TGTGCAGGAGACAGGTATAATAGTGGCGGCGGAGACTACTGG CAGDRYNSGGGDYW IGHV6-1 IGHD5-12 IGHJ4 11 19 27 33
1 0.02564102564102564 TGTGCGAGAGATAGTGGGGGTATGGACGTCTGG CARDSGGMDVW IGHV7-4-1 IGHD1-26 IGHJ6 9 10 18 18
1 0.02564102564102564 TGTTCGACCCTCCAACCGCGTATTGCAGCACCTGAATTCTGG CSTLQPRIAAPEFW IGHV7-4-1 IGHD6-13 IGHJ1 7 24 30 38
1 0.02564102564102564 TGTGCGAGAGGAAGTTACAATTATTGGAGTGATTATTGTTCCCACCTTGACTACTGG CARGSYNYWSDYCSHLDYW IGHV4-59 IGHD3-3 IGHJ1 10 23 37 52
1 0.02564102564102564 TGTGCAAGAGGTCGCGAACAAGATCTTCCGTCTGCTGACTTTAACCAGTGG CARGREQDLPSADFNQW IGHV6-1 IGHD1/OR15-1a IGHJ1 10 15 21 48
1 0.02564102564102564 TGTGCGAGAGAGGGATATTATATTGATACAACTGGTTCTCCGAGGTCACACGGTTTGGACGTCTGG CAREGYYIDTTGSPRSHGLDVW IGHV7-4-1 IGHD1-1 IGHJ6 9 26 35 49
1 0.02564102564102564 TGCGCGAGGGCCCGTGGTGATGGTTATTATAATGATCGATTACGATTTGACTTGTGG CARARGDGYYNDRLRFDLW IGHV4-34 IGHD3-9 IGHJ4 8 20 32 45
1 0.02564102564102564 TGTGCGAGCCTCAAGAGGGGAGGAGACTTCTACTACGCTTTGGACGTCTGG CASLKRGGDFYYALDVW IGHV4-59 IGHD3-10 IGHJ6 8 16 22 29
1 0.02564102564102564 TGTGCGAGGGGATATTCCTATGATCTCTATGGCAATAAAGGAGGTTTTGACTATTGG CARGYSYDLYGNKGGFDYW IGHV4-59 IGHD2-2 IGHJ4 8 9 16 45
1 0.02564102564102564 TGTGCAAGAGACAAGAGTCGGGATGACTACTATGACCACTGG CARDKSRDDYYDHW IGHV6-1 IGHD3-10 IGHJ4 11 17 22 25
1 0.02564102564102564 TGTGCGAGAGATCTGCCAGTGGGTGGAACGACACCGTCTGACTACTGG CARDLPVGGTTPSDYW IGHV7-4-1 IGHD1-1 IGHJ4 9 23 32 38
1 0.02564102564102564 TGTGGAAGAGAGAGTCTCGGCCACTGGATTGACTATTGG CGRESLGHWIDYW IGHV7-4-1 IGHD1-20 IGHJ4 9 22 28 28
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