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October 26, 2011 08:26
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CuffDiff output iterator, preliminar example
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#CLASS DEFINITION Bio::Ngs::Cufflinks | |
class Diff | |
include Bio::Command::Wrapper | |
include Bio::Ngs::Cufflinks::Diff::Utils | |
set_program Bio::Ngs::Utils.binary("cuffdiff") | |
add_option "output-dir", :type => :string, :aliases => '-o', :default => "./" | |
add_option "time-series", :type => :boolean, :aliases => '-T' | |
add_option "min-alignment-count", :type => :numeric, :aliases => '-c' | |
add_option "FDR", :type => :numeric, :aliases => '-F' | |
#TODO:FIX add_option "mask-file", :type => :string, :aliases => '-M' | |
#TODO:FIX add_option "frag-bias-correct", :type => | |
add_option "multi-read-correct", :type => :boolean, :aliases => '-u' | |
add_option "upper-quartile-norm", :type => :boolean, :aliases => 'N' | |
add_option "labels", :type => :array, :aliases => '-L' | |
add_option "num-threads", :type => :numeric, :aliases => '-p' | |
add_option "library-type", :type => :string, :aliases => '-l' | |
add_option "frag-len-mean", :type => :numeric, :aliases => '-m' | |
add_option "frag-len-std-dev", :type => :numeric, :aliases => '-s' | |
add_option "num-importance-samples", :type => :numeric, :aliases => '-i' | |
add_option "max-mle-iterations", :type => :numeric, :aliases => '-e' | |
add_option "compatible-hits-norm", :type => :boolean, :aliases => '-h' | |
add_option "total-hits-norm", :type => :boolean, :aliases => '-t' | |
add_option "poisson-dispersion", :type => :boolean, :aliases => '-d' | |
add_option "verbose", :type => :boolean, :aliases => '-v' | |
add_option "quiet", :type => :boolean, :aliases => '-q' | |
add_option "no-update-check", :type => :boolean, :aliases => '-j' | |
add_option "emit-count-tables", :type => :boolean, :aliases => '-b' | |
#define iterators | |
add_iterator_for :genes | |
add_iterator_for :isoforms | |
add_iterator_for :cds | |
add_iterator_for :tss_groups | |
end #Diff | |
#HOW TO USE THE functionality | |
Bio::Ngs::Cufflinks::Diff.foreach_gene_tracked("path_to_cuffdiff_output_directory") do |gene_fpkm_track| | |
expression_profile = (1..7).map do |sample_idx| | |
gene_fpkm_track["q#{sample_idx}_FPKM"].to_f | |
end | |
#do your stuff accessing this tabular file with gene_fpkm_track["name of the field"] | |
end |
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