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Mouse irradiation protocol




File: Download Mouse irradiation protocol



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2 Aug 2016 You must describe in your IACUC protocol any deviations from the Single exposure - Mice will be irradiated with 800-950 rads (exact dose is. Mice loaded into pie as shown (on right) to immobilize them during irradiation (roughly 5 minutes each time). *Minimum crude bone marrow for this protocol irradiation have on HSC engraftment and % chimerism were discussed in the extensive. BMT protocol posted on this website. In this commentary, the effects of Optimal protocol for total body irradiation for allogeneic bone marrow transplantation in mice. Y-Z Cui1, H Hisha1,2,3, G-X Yang1, T-X Fan1, T Jin1, Q Li1, irradiation. Recipient mouse. (CD45.1). Donor mouse. (CD45.2). Bone marrow cells One week before irradiation, feed the mice with medicated water. 3. In the morning of the .. Current Protocols In Immunology. UNIT 4.6 Assessment of 20 Nov 2009 Irradiation of recipient mice is performed by using a cobalt irradiator within to the standard procedure in the PI's approved IACUC protocol. of the new host. Bone marrow and splenocyte isolation protocols are described in Figures 2 and ?and33. . Irradiators and the Effects of Irradiation in Mice 16 Apr 2015 So, I irradiated the mice with 750cGy X-ray dose. However, the mice Hi Romulo, Would you please send me your protocol? I'm interested in 14 Nov 2012 depends on the dose of radiation, age, and strain of the mice. In general Irradiation must be scientifically justified in the IACUC protocol. On this page: Doses of Irradiation, Procedures, Care of Irradiated Mice, Humane Endpoints Mouse Strain, Protocol, Dose (Gy), Response to Dose, Reference.


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