node.jsでBuffer経由でArrayBufferを使う ref: http://qiita.com/items/899e2ce3ec636869196c

file0.txt

var fs = require('fs');

function toArrayBuffer(buffer) {
    var ab = new ArrayBuffer(buffer.length);
    var view = new Uint8Array(ab);
    for (var i = 0; i < buffer.length; ++i) {
        view[i] = buffer[i];
    }
    return ab;
}

var buf = fs.readFileSync('./hoge.bin');
var arrayBuf = toArrayBuffer(buf);