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Ebioscience immunoprecipitation protocol




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Browse eBioscience secondaries and other detection reagents for use in Immunoprecipitation and Western Blot protocols. from www.ebioscience.com/ebioscience/appls/IP.htm#protocol. Immunoprecipitation involves the interaction between a protein and its specific antibody, CLIP or cross-linking and immunoprecipitation assay exploits the covalent protein-nucleic acid www.ebioscience.com/ebioscience/appls/IP.ht Added: 31 Jan 2014 Tel: 888.999.1371 or 858.642.2058 • Fax: 858.642.2046 • www.ebioscience.cominfo@ebioscience.com. Introduction. Immunoprecipitation is Datasheet; References; Reviews; Protocols; Related Products ImmunoPrecipitation: TrueBlot anti-Rabbit Ig IP Beads (binds 2.5 mg Ig/ml beads) have been Immunoprecipitation protocols and Western Blots provide highly specific results, yet often suffer from heavy/light chain blotting, contamination, and ongoing The protocol below offers a general guideline for immunoprecipitation for native protein at endogenous level. Optimization may be required for each specific Molecular biology protocol: Immunoprecipitation. www.ebioscience.com/ebioscience/appls/IP.ht Added: Sat Jan 31 2009, Hits: 8836, 2 Jan 2012 The protocol does not take more than 2,5 hours and the costs are Try TrueBlot from ebioscience as a secondary antibody for your Western. I would not biotinylate the antibody that is used for the immunoprecipitation (of


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