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22 Apr 2013 24 Nov 2014 Double Digest Protocol with Standard Restriction Enzymes. the two enzymes exhibit > 50% activity, this sequential digest can be performed. See the NEB Activity/Performance Chart for the incubation temperatures. Over 180 of our restriction enzymes are able to digest DNA in. 5–15 minutes, and can . As a solution, NEB offers a line of High Fidelity (HF™) restriction enzymes that have been engineered for . the recommended protocol. Lane L is the Restriction Enzyme Digestion z uL Restriction Enzyme(s) (keep on ice!) 2 , 3 1= Many NEB enzymes now work in the new buffer system called CutSmart. 7 Dec 2012 Standard Protocol for Restriction Enzyme Digests. Let one of NEB's restriction enzyme experts help you improve your technique and avoid Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated Over 210 restriction enzymes are 100% active in a single buffer, CutSmart Buffer, making it significantly easier to set up your double digest reactions. 7 May 2014 NEB Restriction Enzyme Double Digest Protocol NEB's online tools, Double Digest Finder and NEBcloner will help guide your reaction buffer 11 Oct 2016 Protocol, tips, and FAQ for how to perform a restriction digest of plasmid For a list of many commonly used restriction enzymes, visit NEB. DNA double digestion protocol. Materials: Restriction enzymes (EcoRI or SpeI or XbaI or PstI). 0 DNA for enzymes of NEB, 4h for enzymes of Takara). 4.
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