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macro "Ratiometric FRET" { | |
path = getDirectory("Choose a Directory"); | |
list = getFileList(path); | |
length = list.length; | |
File.makeDirectory(path + "Imaging/"); | |
//FRET map creation |
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Dear GitHub community | |
I was wondering if someone can help me to improve a Macro that I found here https://imagej.nih.gov/ij/macros/SubractMeasuredBackground.txt | |
I do not know how to write proper macros so I am trying to adapt it. | |
I would like to do a background subtraction from an ROI to every single image of a folder, but I would like to have the" waitforuser" command in this macro because every image has different element on it, and I will have to choose every time a different spot in the image to draw a ROI. | |
Furthermore, I would like to save these processed images in another folder as Tiff or as the original file from Zeiss .LSM. | |
So far the macro runs and opens and processed only the first image. | |
/ This macro subtracts the mean gray level calculated |