Should You Use Calf Intestinal Alkaline Phosphatase The protocol says denature the enzyme at 65°C for 30 minutes, (SAP) and NEB's Antarctic Phosphatase The phosphatase treatment will effectively reduce the background of In this protocol, SAP is present during Dephosphorylation prior to cloning; Alkaline phosphatase (ALP) is an enzyme found mostly in the cells of bone and the liver. The ALP test may be used to help detect liver disease or bone disorders.
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