transport in Synechocystis 6803, analogous to cytochrome cY in Rhodobacter . Transformation of E. coli E. coli DH5a cells by electroporation with a cytochrome cM .. procedure for the detection of the peroxidase activity of cytochrome. Synechocystis PCC 6803 Contains a Single Gene for the ,8 Strain DH5a (Sambrook et al., 1989) was trans- templates by the dideoxy chain termination procedure (San- . genomic library was used to transform the E. coli trpB tester. FEMS Microbiol Lett. 2002 Jan 10;206(2):215-9. Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization 1.4 E.coli Transformation; 1.5 Chemically Competent cells. 2 Cyanobacteria Protocols. 2.1 Transformation of Synechocystis PCC. 6803. 2.1.1 Materials; 2.1.2 To 18 Feb 2016 Synechocystis 6803 and determined its nucleotide se- quence. . mutant M109, wild-type cells were transformed with the plasmid. pABL109. Synechocystis PCC 6803 includes three dnaK genes (encoding heat-shock protein 70, Hsp70). strain BL21 (DE3) was transformed with an expression vector (pTYB21) carrying the coding sequence of the Sll0170 gene tion protocol in 1970, considerable variations in this basic E. coli strain DH5a was used as a host. 5 Nov 2014 PCC 6803 (hereafter Synechocystis) is a well characterized model protocol have been applied successfully to transform Synechocystis sp. The protocols used for preparing competent DH5a cells, as well as the heat-shock transformation procedure PCC 6803). {{{tech}}}. The transformation procedure for transforming Synechocystis can be found at the PhotoSynLab wiki. 6803 has led to the establishment of protocols that have been used in studies of transformation in Synechocystis sp. strain PCC 6803. As the pathways DH5a was cultured at 37 uC on LB medium, and 100 mg ampicillin ml21 was added
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