The TRAFO Page . HIGH EFFICIENCY TRANSFORMATION Reference: Gietz, R.D. and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER DNA/PEG METHOD. YEAST TRANSFORMATION PROTOCOL. Note: This is a simplified (i.e. sloppier) version of a protocol by Gietz. If you need to troubleshoot, refer to the more detailed This is a quite simple but reliable protocol to make very high transformation efficiency yeast competent cells. By express your gene of interest, protein function can Electroporation Protocol (C2986) Heat-denatured ligation reactions can be used for electroporation directly; however, column purification is recommended. Wittrup: Yeast Transformation. precipitate DNA for transformation using PelletPaint according to the manufacturer's protocol. Typically, four electroporation Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. Before you start this protocol you should have your gene of interest cloned yeast deletion strains were derived from strain BY4742 The following protocol is a slight modification of the "Quick and Dirty" transformation Expression of Recombinant Genes in the Yeast Pichia pastoris. Transformation by Electroporation; Alternate Protocol 1: Rapid Preparation of Electrocompetent Cells; Electroporation of Pichia pastoris - what parameters do But the transformed yeast were viable the Pichia pastoris electroporation. I performed a protocol This protocol describes a method for transforming yeast with DNA by electroporation. Yeast cells are made competent, mixed with the DNA to be introduced, and then Electroporation Apparatus Operating Instructions and Applications Guide The MicroPulser system is used for the electroporation of bacteria, yeast, and other Electroporation Apparatus Operating Instructions and Applications Guide The MicroPulser system is used for the electroporation of bacteria, yeast, and other Preparation of Yeast Frozen Competent Cells. Adapted From: Frozen competent yeast cells that can be transformed with high according to the protocol described Bacterial Transformation: Electroporation. Also it is limited to bacterial, yeast and plant protoplasts while electroporation can be applied to mammalian cells. Electroporation of Pichia 2003-09-17 (Based on Invitrogen protocol) Materials • 5-100 ug pure plasmid DNA containing gene of interest, linearized by restriction
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