#Pearson's Coefficient Range between -1 and 1. -1 is a negative correlation and +1 is a positive correlation.
Scatter plots and PCs point to colocalization especially where it is complete (Fig. 5A and B); however, they rarely discriminate differences between partial colocalization or exclusion, especially if images contain noise.
Mander's removes mean fluorescent intensity from Pearson's, which gives a new value between 0 and 1.0, where 1.0 is 100% colocalization.