fastqc_data.txt

fastqc_data.txt

##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	sample1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1571332	
Filtered Sequences	0	
Sequence length	29	
%GC	53	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.851088121415465	40.0	40.0	40.0	40.0	40.0
2	39.823216863145404	40.0	40.0	40.0	40.0	40.0
3	39.806648754050705	40.0	40.0	40.0	40.0	40.0
4	39.7966120463403	40.0	40.0	40.0	40.0	40.0
5	39.48240982809489	40.0	40.0	40.0	40.0	40.0
6	39.687211868656654	40.0	40.0	40.0	40.0	40.0
7	39.614472943973645	40.0	40.0	40.0	40.0	40.0
8	39.53903821725771	40.0	40.0	40.0	40.0	40.0
9	39.40539300415189	40.0	40.0	40.0	40.0	40.0
10	39.22727533073851	40.0	40.0	40.0	40.0	40.0
11	39.03652060799373	40.0	40.0	40.0	40.0	40.0
12	38.83684733716363	40.0	40.0	40.0	40.0	40.0
13	38.00679105370475	40.0	40.0	40.0	40.0	40.0
14	37.420608121008165	40.0	40.0	40.0	32.0	40.0
15	37.22028126455771	40.0	40.0	40.0	29.0	40.0
16	36.47825539096766	40.0	40.0	40.0	22.0	40.0
17	35.04345039749715	40.0	40.0	40.0	13.0	40.0
18	35.44634106605097	40.0	40.0	40.0	16.0	40.0
19	34.87004465001667	40.0	40.0	40.0	14.0	40.0
20	34.15579457428475	40.0	38.0	40.0	11.0	40.0
21	33.22712768530139	40.0	31.0	40.0	10.0	40.0
22	32.49217288262442	40.0	27.0	40.0	9.0	40.0
23	31.41095961897295	40.0	22.0	40.0	7.0	40.0
24	30.57499560882105	40.0	20.0	40.0	6.0	40.0
25	29.429261289148315	40.0	16.0	40.0	5.0	40.0
26	28.152521554961012	40.0	14.0	40.0	5.0	40.0
27	26.803150448154813	35.0	11.0	40.0	4.0	40.0
28	25.574543762871247	30.0	10.0	40.0	4.0	40.0
29	24.970248808017658	28.0	10.0	40.0	4.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	89.0
5	183.0
6	305.0
7	464.0
8	685.0
9	800.0
10	1113.0
11	1298.0
12	1651.0
13	2041.0
14	2287.0
15	2835.0
16	3302.0
17	3975.0
18	4871.0
19	6317.0
20	8191.0
21	9260.0
22	10541.0
23	12046.0
24	13382.0
25	14679.0
26	16491.0
27	19535.0
28	23042.0
29	27671.0
30	35563.0
31	44664.0
32	57706.0
33	74274.0
34	98024.0
35	128284.0
36	167036.0
37	214255.0
38	258030.0
39	191648.0
40	114786.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.241873773333708	25.50345821252288	21.963786138129944	29.29088187601347
2	27.169624242362534	25.781948054262244	24.269791489004234	22.77863621437099
3	23.120129927984664	24.72106467633829	24.628658997589305	27.530146398087734
4	26.852822955301615	23.65559919864166	22.953965170950504	26.537612675106214
5	27.25070195222906	25.260925125944105	23.557274974352968	23.931097947473862
6	26.298261602258467	24.31013942311364	22.162916557417528	27.228682417210365
7	26.202101147306873	24.210924234980258	24.084216448210817	25.502758169502055
8	25.566016602474846	23.617733235242458	24.450020746729525	26.36622941555317
9	25.792957821771594	22.66802941708054	24.320640068426023	27.218372692721843
10	26.42038728925523	23.332306603569457	22.75986233335794	27.487443773817372
11	27.0441892610855	22.35141905084349	23.14405867124198	27.460333016829036
12	27.105283924721192	22.019025896500548	23.555493046663596	27.320197132114664
13	26.635085749636268	23.10562231972719	23.420667743549206	26.83862418708734
14	26.172319182455045	23.24820212858802	23.70960642080097	26.86987226815597
15	26.1976529358681	23.250608036978328	23.55381453646216	26.997924490691418
16	26.32957630017703	23.102791688193218	23.37346880142085	27.194163210208906
17	26.474843293642607	23.217063768555963	23.46397927103485	26.84411366676657
18	26.470665347656574	22.88785195539836	23.425580909055448	27.215901787889617
19	26.453081779120737	22.81294584910698	23.190748704707783	27.5432236670645
20	26.742598667454516	22.834995619307648	23.40060158112431	27.02180413211353
21	26.951538135231573	22.316049879682907	23.118206288080774	27.614205697004746
22	27.058011471885727	22.381256813342436	23.240120016708605	27.320611698063228
23	26.92535865553664	22.01493819883565	23.293545664370473	27.76615748125723
24	27.042338684012883	21.60921369196889	23.55213411917461	27.796313504843617
25	27.081187478435353	21.635628427267136	23.95085997588579	27.332324118411723
26	26.97726779635417	21.107752526884852	24.180334426242098	27.73464525051888
27	26.952537045982005	20.576562545801668	24.012818731814747	28.45808167640158
28	28.15846909221833	19.935065141961363	23.843327927405504	28.063137838414796
29	28.972366505788987	19.056399263480213	24.658646756276998	27.3125874744538
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	52.53275564934717
2	49.94826045673352
3	50.65027632607241
4	53.39043563040783
5	51.18179989970293
6	53.526944019468836
7	51.70485931680893
8	51.93224601802802
9	53.01133051449344
10	53.90783106307261
11	54.50452227791453
12	54.42548105683586
13	53.4737099367236
14	53.04219145061101
15	53.19557742655952
16	53.52373951038594
17	53.31895696040918
18	53.68656713554619
19	53.99630544618523
20	53.764402799568046
21	54.565743832236315
22	54.37862316994896
23	54.69151613679387
24	54.83865218885649
25	54.41351159684707
26	54.71191304687305
27	55.41061872238359
28	56.22160693063313
29	56.28495398024279
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5361.0
1	5361.0
2	3338.5
3	1316.0
4	1316.0
5	907.0
6	498.0
7	498.0
8	498.0
9	514.5
10	531.0
11	531.0
12	708.5
13	886.0
14	886.0
15	886.0
16	1426.5
17	1967.0
18	1967.0
19	3047.5
20	4128.0
21	4128.0
22	6244.5
23	8361.0
24	8361.0
25	8361.0
26	11779.0
27	15197.0
28	15197.0
29	20793.0
30	26389.0
31	26389.0
32	26389.0
33	34922.5
34	43456.0
35	43456.0
36	55711.0
37	67966.0
38	67966.0
39	67966.0
40	83327.5
41	98689.0
42	98689.0
43	115471.0
44	132253.0
45	132253.0
46	132253.0
47	148537.5
48	164822.0
49	164822.0
50	175053.0
51	185284.0
52	185284.0
53	188327.0
54	191370.0
55	191370.0
56	191370.0
57	185090.0
58	178810.0
59	178810.0
60	164259.0
61	149708.0
62	149708.0
63	149708.0
64	132238.5
65	114769.0
66	114769.0
67	97044.0
68	79319.0
69	79319.0
70	79319.0
71	64439.0
72	49559.0
73	49559.0
74	38590.0
75	27621.0
76	27621.0
77	27621.0
78	20615.0
79	13609.0
80	13609.0
81	9833.0
82	6057.0
83	6057.0
84	4176.0
85	2295.0
86	2295.0
87	2295.0
88	1497.0
89	699.0
90	699.0
91	445.0
92	191.0
93	191.0
94	191.0
95	138.5
96	86.0
97	86.0
98	110.5
99	135.0
100	135.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.008273235700666695
14	0.09921518813337984
15	0.07095890620187204
16	0.09959702978110292
17	1.903735174998027
18	0.47940218871632473
19	0.12696234786792351
20	0.05116678079489248
21	0.08362332085135414
22	0.05625800276453353
23	0.020746729526287252
24	0.012218932727138504
25	0.03016549017012318
26	0.5622618262722328
27	0.13148080736597995
28	0.13154444764060044
29	0.010118803664661573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
29	1571332.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	7.85838291810722
#Duplication Level	Relative count
1	100.0
2	6.711005899372487
3	0.5163963905932117
4	0.07354085811982329
5	0.022008577977465364
6	0.011809480865957024
7	0.004831151263346056
8	0.004294356678529827
9	5.367945848162284E-4
10++	0.014493453790038167
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5226	0.33258407516680116	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	247430	10.240459	11.52296	3
TTTTT	91310	3.2113638	5.350794	25
>>END_MODULE