（解決済み：原因はコード１行漏れ）Python から Gmail にメール送信 拒否される件 ～Gmail アカウント設定したのになぜ？ ref: http://qiita.com/HirofumiYashima/items/1b24397c2e915658c984

file1.txt

  smtp.login(self.username, self.password)
    smtp.mail(self.username)
    smtp.rcpt(to)

mail.py(修正後)

File Edit Options Buffers Tools Python Help                                                            
#!/Usr/bin/env python                                                                                  
# -*- coding: utf-8 -*-                                                                                

import smtplib
from email.mime.text import MIMEText

class sendGmail:
    username, password = 'hirofumi.yashima2012@gmail.com', 'XXXXX'

    def __init__(self, to, sub, body):
        host, port = 'smtp.gmail.com', 465
        msg = MIMEText(body)
        msg['Subject'] = sub
        msg['From'] = self.username
        msg['To'] = to

        smtp = smtplib.SMTP_SSL(host, port)
        smtp.ehlo()
        smtp.login(self.username, self.password)
        smtp.mail(self.username)
        smtp.rcpt(to)
        smtp.data(msg.as_string())
        smtp.quit()

if __name__ == '__main__':
    to = 'hirofumi.yashima2012@hotmail.co.jp'
    sub = 'Python smtplib'
    body = 'Hello, Python'
    sendGmail(to, sub, body)

mail_japanese_lang.py

File Edit Options Buffers Tools Python Help                                                            
#!/Usr/bin/env python                                                                                  
# -*- coding: utf-8 -*-                                                                                

import smtplib
from email.mime.text import MIMEText

class sendGmail:
    username, password = 'hirofumi.yashima2012@gmail.com', 'XXXXX'

    def __init__(self, to, sub, body):
        host, port = 'smtp.gmail.com', 465
        msg = MIMEText(body)
        msg['Subject'] = sub
        msg['From'] = self.username
        msg['To'] = to

        smtp = smtplib.SMTP_SSL(host, port)
        smtp.ehlo()
        smtp.login(self.username, self.password)
        smtp.mail(self.username)
        smtp.rcpt(to)
        smtp.data(msg.as_string())
        smtp.quit()

if __name__ == '__main__':
    to = 'hirofumi.yashima2012@gmail.com'
    sub = 'Pythonから日本語初メール'
    body = 'やあ！ Python！元気かい？\n今日は暑いね〜。でも、明日からまた寒くなるみたいよ。'
    sendGmail(to, sub, body)

Python 2.7

#!/Usr/bin/env python
# -*- coding: utf-8 -*-

import smtplib
from email.mime.text import MIMEText

class sendGmail:
    username, password = 'hirofumi.yashima2012@gmail.com', 'XXXXX'

    def __init__(self, to, sub, body):
        host, port = 'smtp.gmail.com', 465
        msg = MIMEText(body)
        msg['Subject'] = sub
        msg['From'] = self.username
        msg['To'] = to

        smtp = smtplib.SMTP_SSL(host, port)
        smtp.ehlo()
        smtp.login(self.username, self.password)
        smtp.rcpt(to)
        smtp.data(msg.as_string())
        smtp.quit()

if __name__ == '__main__':
    to = 'hirofumi.yashima2012@gmail.com'
    sub = 'Python smtplib'
    body = 'Hello, Python'
    sendGmail(to, sub, body)