Created
October 1, 2010 20:40
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An example of using both paver and ruffus to do scientific workflows.
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from paver.easy import * | |
import os.path, os | |
import csv | |
import ruffus | |
from collections import defaultdict | |
from types import GeneratorType, DictType | |
from itertools import ifilter | |
options( | |
DATA_DIR = 'Data', | |
PROCESSED = 'Data/Processed', | |
STITCH_CUT = 900, | |
) | |
@task | |
def touch_data(): | |
for path, _, files in os.walk(options.DATA_DIR): | |
for f in files: | |
f = f.replace(' ', '\ ') | |
sh('touch %s' % os.path.join(path, f)) | |
@task | |
def run(): | |
ruffus.pipeline_run([top_function]) | |
@ruffus.follows('process_data', 'process_stitch') | |
def top_function(): | |
pass | |
@ruffus.merge(os.path.join(options.PROCESSED, '*.csv'), | |
os.path.join(options.PROCESSED, 'results.out')) | |
@ruffus.follows('process_bras', 'process_chassey', 'process_fu', | |
'process_konig', 'process_kumar', 'process_shapira', | |
'process_tai') | |
def process_data(in_files, out_file): | |
fields = ('Symbol', 'Viral-Protein', | |
'Disease', 'Method', 'Ref') | |
with open(out_file, 'w') as out_handle: | |
writer = csv.DictWriter(out_handle, fields, | |
delimiter = '\t') | |
for f in in_files: | |
with open(f) as in_handle: | |
writer.writerows(csv.DictReader(in_handle, delimiter = '\t')) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Bras-2008', 'Table-S2.csv'), | |
os.path.join(options.PROCESSED, 'Bras-2008.csv')) | |
def process_bras(in_file, out_file): | |
def conv_fun(row): | |
return { | |
'Symbol':row["Symbol"], | |
'Viral-Protein':'Unknown', | |
'Disease':'HIV', | |
'Method':'RNAi', | |
'Ref':'Bras-2008' | |
} | |
process_file(in_file, out_file, conv_fun) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Chassey-2008', 'msb200866-s2.csv'), | |
os.path.join(options.PROCESSED, 'Chassey-2008.csv')) | |
def process_chassey(in_file, out_file): | |
def conv_fun(row): | |
if len(row["Text Mining"].strip()) > 0: | |
yield { | |
'Symbol':row["Gene Symbol"], | |
'Viral-Protein':row["HCV-Protein"], | |
'Disease':'HCV', | |
'Method':'Literature', | |
'Ref':'Chassey-2008' | |
} | |
if len(row["Y2H"].strip()) > 0: | |
yield { | |
'Symbol':row["Gene Symbol"], | |
'Viral-Protein':row["HCV-Protein"], | |
'Disease':'HCV', | |
'Method':'Y2H', | |
'Ref':'Chassey-2008' | |
} | |
process_file(in_file, out_file, conv_fun) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Fu-2009', 'hiv_interactions'), | |
os.path.join(options.PROCESSED, 'Fu-2009.csv')) | |
def process_fu(in_file, out_file): | |
def conv_fun(row, symbol_id): | |
return { | |
'Symbol':symbol_id[row["Gene ID 2"]], | |
'Viral-Protein':row["product name 1"], | |
'Disease':'HIV', | |
'Method':'Literature', | |
'Ref':'Fu-2009' | |
} | |
symbol_id = get_geneID2Symbol() | |
process_file(in_file, out_file, conv_fun, extra = (symbol_id, )) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Konig-2010', 'nature08699-s8.csv'), | |
os.path.join(options.PROCESSED, 'Konig-2010.csv')) | |
def process_konig(in_file, out_file): | |
def conv_fun(row): | |
if len(row["Influenza (this study)"]) > 0: | |
return { | |
'Symbol':row["Symbol"], | |
'Viral-Protein':'Unknown', | |
'Disease':'Influenza', | |
'Method':'RNAi', | |
'Ref':'Konig-2010' | |
} | |
process_file(in_file, out_file, conv_fun) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Kumar-2010', 'mmc2.csv'), | |
os.path.join(options.PROCESSED, 'kumar-2010.csv')) | |
def process_kumar(in_file, out_file): | |
def conv_fun(row): | |
if float(row["p-value"]) < 0.05: | |
return { | |
'Symbol':row["GeneSymbol"], | |
'Viral-Protein':'Unknown', | |
'Disease':'Influenza', | |
'Method':'RNAi', | |
'Ref':'Kumar-2010' | |
} | |
process_file(in_file, out_file, conv_fun) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Shapira-2009', 'mmc2.csv'), | |
os.path.join(options.PROCESSED, 'Shapira-2009.csv')) | |
def process_shapira(in_file, out_file): | |
def conv_fun(row, symbol_id, pdict): | |
gene = symbol_id[row['entrez gene ID']] | |
yield { | |
'Symbol':gene, | |
'Ref':'Shapira-2009', | |
'Disease':'Influenza', | |
'Method':'Y2H', | |
'Viral-Protein':'Unknown' | |
} | |
for field, info in pdict.items(): | |
if len(row[field].strip()) > 0: | |
yield dict(Symbol = gene, **info) | |
pdict = { | |
'HCV Li et al. (25 genes)': { | |
'Ref':'Li-2009', | |
'Disease':'HCV', | |
'Method':'RNAi' | |
}, | |
"WNV Krishnan et al. (14 genes)":{ | |
'Ref':'Krishnan-2008', | |
'Disease':'West-Nile', | |
'Method':'RNAi' | |
}, | |
"HIV Zhou et al. (5 genes)":{ | |
'Ref':'Zhou-2008', | |
'Disease':'HIV', | |
'Method':'RNAi' | |
} | |
} | |
symbol_id = get_geneID2Symbol() | |
process_file(in_file, out_file, conv_fun, extra = (symbol_id, pdict)) | |
@ruffus.files(os.path.join(options.DATA_DIR, 'Tai-2009', 'mmc3.csv'), | |
os.path.join(options.PROCESSED, 'Tai-2009.csv')) | |
def process_tai(in_file, out_file): | |
def conv_fun(row): | |
return { | |
'Symbol':row['Symbol'], | |
'Ref':'Tai-2009', | |
'Disease':'HCV', | |
'Method':'RNAi', | |
'Viral-Protein':'Unknown' | |
} | |
process_file(in_file, out_file, conv_fun) | |
@ruffus.files([os.path.join(options.DATA_DIR, 'stitch', 'protein.aliases.v8.2.txt'), | |
os.path.join(options.DATA_DIR, 'stitch', 'protein_chemical.human.links.v2.0.tsv'), | |
os.path.join(options.DATA_DIR, 'stitch', 'chemical.aliases.v2.0.tsv'),], | |
os.path.join(options.DATA_DIR, 'stitch', 'processed.csv')) | |
def process_stitch(in_files, out_file): | |
protein_alias, protein_chemical, chemical_alias = in_files | |
symbol_id = get_geneID2Symbol(source = 'symbol') | |
print 'getting protein-conv' | |
protein_conv = {} | |
with open(protein_alias) as handle: | |
fields = ('species', 'protein-id', 'alias', 'source') | |
handle.next() | |
for row in csv.DictReader(handle, fieldnames = fields, delimiter = '\t'): | |
if row['species'] == '9606' and row['source'] == 'Ensembl_EntrezGene': | |
protein_conv[row['protein-id']] = symbol_id[row['alias']] | |
print 'getting chem-conv' | |
chem_conv = {} | |
with open(chemical_alias) as handle: | |
for row in csv.DictReader(handle, delimiter = '\t'): | |
chem_conv[row['chemical']] = row['alias'] | |
fields = ('Symbol', 'Chemical') | |
in_fields = ('chemical', 'protein', 'combined_score') | |
print 'processing interactions' | |
with open(out_file, 'w') as out_handle: | |
writer = csv.DictWriter(out_handle, fields, delimiter = '\t') | |
with open(protein_chemical) as handle: | |
for c, row in enumerate(csv.DictReader(handle, | |
fieldnames = in_fields, | |
delimiter = '\t')): | |
if c % 10000 == 0: | |
print c | |
if row['protein'].startswith('9606.'): | |
prot = row['protein'].split('.', 1)[1] | |
if int(row['combined_score']) > options.STITCH_CUT: | |
gene = protein_conv.get(prot, prot) | |
chem = chem_conv.get(row['chemical'], row['chemical']) | |
writer.writerow({ | |
'Symbol':gene, | |
'Chemical':chem | |
}) | |
def get_geneID2Symbol(source = 'geneid'): | |
fname = os.path.join(options.DATA_DIR, 'Homo_sapiens.gene_info') | |
fields = ('taxid', 'geneid', 'symbol', 'locustag', 'synonyms', | |
'dbXrefs', 'chromosome', 'maplocation', 'description', | |
'type', 'symauth', 'fullauth', 'status', 'other', 'date') | |
out = defaultdict(lambda : None) | |
with open(fname) as handle: | |
for row in csv.DictReader(handle, fieldnames = fields, delimiter = '\t'): | |
if source == 'symbol': | |
for val in row[source].split('|'): | |
out[val] = row['symbol'] | |
else: | |
out[row[source]] = row['symbol'] | |
return out | |
def process_file(in_file, out_file, func, extra = tuple()): | |
fields = ('Symbol', 'Viral-Protein', | |
'Disease', 'Method', 'Ref') | |
with open(in_file) as in_handle: | |
line_gen = csv.DictReader(in_handle, delimiter = '\t') | |
with open(out_file, 'w') as out_handle: | |
out_handle.write('\t'.join(fields)+'\n') | |
writer = csv.DictWriter(out_handle, | |
fields, | |
delimiter = '\t') | |
for row in line_gen: | |
val = func(row, *extra) | |
if type(val) == GeneratorType: | |
writer.writerows(val) | |
elif type(val) == DictType: | |
writer.writerow(val) |
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