Skip to content

Instantly share code, notes, and snippets.

@MBeyens
Created October 26, 2017 10:36
Show Gist options
  • Save MBeyens/f5869c46d42d824429078afffebb9c30 to your computer and use it in GitHub Desktop.
Save MBeyens/f5869c46d42d824429078afffebb9c30 to your computer and use it in GitHub Desktop.
cnvkit segmentation fault
[2017-10-26T09:46Z] System YAML configuration: /mnt/home_nfs1/mbeyens/local/share/bcbio/galaxy/bcbio_system.yaml
[2017-10-26T09:46Z] Resource requests: sambamba, samtools; memory: 2.00, 2.00; cores: 16, 16
[2017-10-26T09:46Z] Configuring 3 jobs to run, using 16 cores each with 32.1g of memory reserved for each job
[2017-10-26T09:46Z] Timing: organize samples
[2017-10-26T09:46Z] multiprocessing: organize_samples
[2017-10-26T09:46Z] Using input YAML configuration: /home/mbeyens/WGS_data/pair_analysis_1/config/run_paired_config.yaml
[2017-10-26T09:46Z] Checking sample YAML configuration: /home/mbeyens/WGS_data/pair_analysis_1/config/run_paired_config.yaml
[2017-10-26T09:46Z] Testing minimum versions of installed programs
[2017-10-26T09:47Z] Timing: alignment preparation
[2017-10-26T09:47Z] multiprocessing: prep_align_inputs
[2017-10-26T09:47Z] multiprocessing: disambiguate_split
[2017-10-26T09:47Z] Timing: alignment
[2017-10-26T09:47Z] multiprocessing: process_alignment
[2017-10-26T09:47Z] Timing: callable regions
[2017-10-26T09:47Z] multiprocessing: prep_samples
[2017-10-26T09:47Z] multiprocessing: postprocess_alignment
[2017-10-26T09:47Z] 161620: Assigned coverage as 'genome' with 89.8% genome coverage and 0.0% offtarget coverage
[2017-10-26T09:47Z] 161624: Assigned coverage as 'genome' with 89.3% genome coverage and 0.0% offtarget coverage
[2017-10-26T09:47Z] 161619: Assigned coverage as 'genome' with 89.7% genome coverage and 0.0% offtarget coverage
[2017-10-26T09:47Z] 161623: Assigned coverage as 'genome' with 89.2% genome coverage and 0.0% offtarget coverage
[2017-10-26T09:47Z] multiprocessing: combine_sample_regions
[2017-10-26T09:47Z] Identified 249 parallel analysis blocks
Block sizes:
min: 4262
5%: 36247.2
25%: 1920597.0
median: 16078400.0
75%: 17607373.0
95%: 22972699.0
99%: 34057170.36
max: 36763413
Between block sizes:
min: 253
5%: 267.0
25%: 315.0
median: 475.5
75%: 1035.0
95%: 25684.0
99%: 10000000.0
max: 10000000
[2017-10-26T09:47Z] multiprocessing: calculate_sv_bins
[2017-10-26T09:47Z] multiprocessing: calculate_sv_coverage
[2017-10-26T09:47Z] Timing: hla typing
[2017-10-26T09:47Z] Resource requests: freebayes, gatk, gatk-haplotype, mutect2, picard, vardict, varscan; memory: 2.00, 3.50, 2.00, 2.00, 2.00, 2.00, 2.00; cores: 16, 1, 16, 16, 16, 16, 16
[2017-10-26T09:47Z] Configuring 48 jobs to run, using 1 cores each with 3.50g of memory reserved for each job
[2017-10-26T09:47Z] Timing: alignment post-processing
[2017-10-26T09:47Z] multiprocessing: piped_bamprep
[2017-10-26T09:47Z] Timing: variant calling
[2017-10-26T09:47Z] multiprocessing: variantcall_sample
[2017-10-26T09:49Z] multiprocessing: concat_variant_files
[2017-10-26T09:49Z] Resource requests: bcbio_variation, fastqc, gatk, gatk-vqsr, gemini, kraken, preseq, qsignature, sambamba, samtools, snpeff; memory: 2.00, 2.00, 3.50, 2.00, 2.00, 2.00, 2.00, 2.00, 2.00, 2.00, 4.00; cores: 16, 16, 1, 16, 16, 16, 16, 16, 16, 16, 1
[2017-10-26T09:49Z] Configuring 2 jobs to run, using 16 cores each with 64.1g of memory reserved for each job
[2017-10-26T09:49Z] Timing: joint squaring off/backfilling
[2017-10-26T09:49Z] Timing: variant post-processing
[2017-10-26T09:49Z] multiprocessing: postprocess_variants
[2017-10-26T09:49Z] Finalizing variant calls: 161620, varscan
[2017-10-26T09:49Z] Calculating variation effects for 161620, varscan
[2017-10-26T09:49Z] Finalizing variant calls: 161624, mutect2
[2017-10-26T09:49Z] Calculating variation effects for 161624, mutect2
[2017-10-26T09:49Z] Annotate VCF file: 161620, varscan
[2017-10-26T09:49Z] Filtering for 161620, varscan
[2017-10-26T09:49Z] Prioritization for 161620, varscan
[2017-10-26T09:49Z] Annotate VCF file: 161624, mutect2
[2017-10-26T09:49Z] Filtering for 161624, mutect2
[2017-10-26T09:49Z] Prioritization for 161624, mutect2
[2017-10-26T09:49Z] Finalizing variant calls: 161620, gatk-haplotype
[2017-10-26T09:49Z] Calculating variation effects for 161620, gatk-haplotype
[2017-10-26T09:49Z] Annotate VCF file: 161620, gatk-haplotype
[2017-10-26T09:49Z] Filtering for 161620, gatk-haplotype
[2017-10-26T09:49Z] Skipping VQSR, using cutoff-based filers: we don't have whole genome input data
[2017-10-26T09:49Z] Prioritization for 161620, gatk-haplotype
[2017-10-26T09:49Z] Finalizing variant calls: 161620, mutect2
[2017-10-26T09:49Z] Calculating variation effects for 161620, mutect2
[2017-10-26T09:49Z] Annotate VCF file: 161620, mutect2
[2017-10-26T09:49Z] Filtering for 161620, mutect2
[2017-10-26T09:49Z] Prioritization for 161620, mutect2
[2017-10-26T09:49Z] Finalizing variant calls: 161620, vardict
[2017-10-26T09:49Z] Calculating variation effects for 161620, vardict
[2017-10-26T09:49Z] Annotate VCF file: 161620, vardict
[2017-10-26T09:49Z] Filtering for 161620, vardict
[2017-10-26T09:49Z] Prioritization for 161620, vardict
[2017-10-26T09:49Z] Finalizing variant calls: 161624, vardict
[2017-10-26T09:49Z] Calculating variation effects for 161624, vardict
[2017-10-26T09:49Z] Annotate VCF file: 161624, vardict
[2017-10-26T09:49Z] Filtering for 161624, vardict
[2017-10-26T09:49Z] Prioritization for 161624, vardict
[2017-10-26T09:49Z] Finalizing variant calls: 161624, gatk-haplotype
[2017-10-26T09:49Z] Calculating variation effects for 161624, gatk-haplotype
[2017-10-26T09:49Z] Annotate VCF file: 161624, gatk-haplotype
[2017-10-26T09:49Z] Filtering for 161624, gatk-haplotype
[2017-10-26T09:49Z] Prioritization for 161624, gatk-haplotype
[2017-10-26T09:49Z] Finalizing variant calls: 161624, freebayes
[2017-10-26T09:49Z] Calculating variation effects for 161624, freebayes
[2017-10-26T09:49Z] Annotate VCF file: 161624, freebayes
[2017-10-26T09:49Z] Filtering for 161624, freebayes
[2017-10-26T09:49Z] Prioritization for 161624, freebayes
[2017-10-26T09:49Z] Finalizing variant calls: 161620, freebayes
[2017-10-26T09:49Z] Calculating variation effects for 161620, freebayes
[2017-10-26T09:49Z] Annotate VCF file: 161620, freebayes
[2017-10-26T09:49Z] Filtering for 161620, freebayes
[2017-10-26T09:49Z] Prioritization for 161620, freebayes
[2017-10-26T09:49Z] Finalizing variant calls: 161624, varscan
[2017-10-26T09:49Z] Calculating variation effects for 161624, varscan
[2017-10-26T09:49Z] Annotate VCF file: 161624, varscan
[2017-10-26T09:49Z] Filtering for 161624, varscan
[2017-10-26T09:49Z] Prioritization for 161624, varscan
[2017-10-26T09:49Z] multiprocessing: split_variants_by_sample
[2017-10-26T09:49Z] Timing: prepped BAM merging
[2017-10-26T09:49Z] Timing: validation
[2017-10-26T09:49Z] multiprocessing: compare_to_rm
[2017-10-26T09:50Z] Timing: ensemble calling
[2017-10-26T09:50Z] Timing: validation summary
[2017-10-26T09:50Z] Timing: structural variation precall
[2017-10-26T09:50Z] Timing: structural variation
[2017-10-26T09:50Z] Timing: structural variation
[2017-10-26T09:50Z] Timing: structural variation ensemble
[2017-10-26T09:50Z] Timing: structural variation validation
[2017-10-26T09:50Z] multiprocessing: validate_sv
[2017-10-26T09:50Z] Timing: heterogeneity
[2017-10-26T09:50Z] Timing: population database
[2017-10-26T09:50Z] multiprocessing: prep_gemini_db
[2017-10-26T09:50Z] Timing: quality control
[2017-10-26T09:50Z] multiprocessing: pipeline_summary
[2017-10-26T09:50Z] QC: 161624 fastqc
[2017-10-26T09:50Z] QC: 161624 qualimap
[2017-10-26T09:50Z] Downsample BAM file: 161624-dedup.bam
[2017-10-26T09:50Z] Downsample BAM file: 161624-dedup.bam
[2017-10-26T10:21Z] FastQC: 161624
[2017-10-26T10:21Z] Prepare merged BED file : 161624
[2017-10-26T10:21Z] bgzip 161624-coverage.callable-vrsubset-callableblocks-merged-merged.bed
[2017-10-26T10:21Z] tabix index 161624-coverage.callable-vrsubset-callableblocks-merged-merged.bed.gz
[2017-10-26T10:21Z] Qualimap: 161624
[2017-10-26T10:21Z] Display:
[2017-10-26T10:21Z] Java memory size is set to 59g
[2017-10-26T10:21Z] Launching application...
[2017-10-26T10:21Z] Started analysis of 161624-dedup-downsample.bam
[2017-10-26T10:21Z] QualiMap v.2.2.2-dev
[2017-10-26T10:21Z] Built on 2016-12-11 14:41
[2017-10-26T10:21Z] Selected tool: bamqc
[2017-10-26T10:21Z] Available memory (Mb): 32
[2017-10-26T10:21Z] Max memory (Mb): 56312
[2017-10-26T10:21Z] Thu Oct 26 12:21:05 CEST 2017 WARNING Output folder already exists, the results will be saved there
[2017-10-26T10:21Z] Starting bam qc....
[2017-10-26T10:21Z] Loading sam header...
[2017-10-26T10:21Z] Loading locator...
[2017-10-26T10:21Z] Loading reference...
[2017-10-26T10:21Z] Only flagged duplicate alignments will be skipped...
[2017-10-26T10:21Z] Number of windows: 400, effective number of windows: 492
[2017-10-26T10:21Z] Chunk of reads size: 1000
[2017-10-26T10:21Z] Number of threads: 16
282
[2017-10-26T10:21Z] Initializing regions from /mnt/home_nfs1/mbeyens/WGS_data/pair_analysis_1/work/qc/161624/qualimap/161624-coverage.callable-vrsubset-callableblocks-merged-merged-bed6.bed.....
cmd)
---> 23 _do_run(cmd, checks, log_stdout, env=env)
cmd = 'unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz'
checks = None
log_stdout = False
env = None
24 except:
25 diagnostics.end_cmd(cmd_id, False)
26 if log_error:
27 logger.exception()
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in _do_run(cmd='set -o pipefail; unset R_HOME && unset R_LIBS &&..._analysis/work/mutect2/1-effects-annotated.vcf.gz', checks=None, log_stdout=False, env=None)
98 error_msg = " ".join(cmd) if not isinstance(cmd, basestring) else cmd
99 error_msg += "\n"
100 error_msg += "".join(debug_stdout)
101 s.communicate()
102 s.stdout.close()
--> 103 raise subprocess.CalledProcessError(exitcode, error_msg)
exitcode = 139
error_msg = 'set -o pipefail; unset R_HOME && unset R_LIBS &&...analysis/work/mutect2/1-effects-annotated.vcf.gz\n'
104 else:
105 break
106 s.communicate()
107 s.stdout.close()
CalledProcessError: Command 'set -o pipefail; unset R_HOME && unset R_LIBS && export PATH=/mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin:$PATH && export TMPDIR=/mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7 && /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
Selected test sample P00132893_tumor and control sample P00132893_normal
/bin/bash: line 1: 65589 Segmentation fault (core dumped) /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
' returned non-zero exit status 139
___________________________________________________________________________
---> 23 _do_run(cmd, checks, log_stdout, env=env)
cmd = 'unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz'
checks = None
log_stdout = False
env = None
24 except:
25 diagnostics.end_cmd(cmd_id, False)
26 if log_error:
27 logger.exception()
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in _do_run(cmd='set -o pipefail; unset R_HOME && unset R_LIBS &&..._analysis/work/mutect2/1-effects-annotated.vcf.gz', checks=None, log_stdout=False, env=None)
98 error_msg = " ".join(cmd) if not isinstance(cmd, basestring) else cmd
99 error_msg += "\n"
100 error_msg += "".join(debug_stdout)
101 s.communicate()
102 s.stdout.close()
--> 103 raise subprocess.CalledProcessError(exitcode, error_msg)
exitcode = 139
error_msg = 'set -o pipefail; unset R_HOME && unset R_LIBS &&...analysis/work/mutect2/1-effects-annotated.vcf.gz\n'
104 else:
105 break
106 s.communicate()
107 s.stdout.close()
CalledProcessError: Command 'set -o pipefail; unset R_HOME && unset R_LIBS && export PATH=/mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin:$PATH && export TMPDIR=/mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7 && /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
Selected test sample P00132893_tumor and control sample P00132893_normal
/bin/bash: line 1: 65589 Segmentation fault (core dumped) /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
' returned non-zero exit status 139
___________________________________________________________________________
mbeyens@trainingvm-n001:/home/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work$
mbeyens@trainingvm-n001:/home/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work$
22 logger_cl.debug(" ".join(str(x) for x in cmd) if not isinstance(cmd, basestring) else cmd)
---> 23 _do_run(cmd, checks, log_stdout, env=env)
cmd = 'unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz'
checks = None
log_stdout = False
env = None
24 except:
25 diagnostics.end_cmd(cmd_id, False)
26 if log_error:
27 logger.exception()
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in _do_run(cmd='set -o pipefail; unset R_HOME && unset R_LIBS &&..._analysis/work/mutect2/1-effects-annotated.vcf.gz', checks=None, log_stdout=False, env=None)
98 error_msg = " ".join(cmd) if not isinstance(cmd, basestring) else cmd
99 error_msg += "\n"
100 error_msg += "".join(debug_stdout)
101 s.communicate()
102 s.stdout.close()
--> 103 raise subprocess.CalledProcessError(exitcode, error_msg)
exitcode = 139
error_msg = 'set -o pipefail; unset R_HOME && unset R_LIBS &&...analysis/work/mutect2/1-effects-annotated.vcf.gz\n'
104 else:
105 break
106 s.communicate()
107 s.stdout.close()
CalledProcessError: Command 'set -o pipefail; unset R_HOME && unset R_LIBS && export PATH=/mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin:$PATH && export TMPDIR=/mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7 && /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
Selected test sample P00132893_tumor and control sample P00132893_normal
/bin/bash: line 1: 65589 Segmentation fault (core dumped) /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
' returned non-zero exit status 139
___________________________________________________________________________
22 logger_cl.debug(" ".join(str(x) for x in cmd) if not isinstance(cmd, basestring) else cmd)
---> 23 _do_run(cmd, checks, log_stdout, env=env)
cmd = 'unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz'
checks = None
log_stdout = False
env = None
24 except:
25 diagnostics.end_cmd(cmd_id, False)
26 if log_error:
27 logger.exception()
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in _do_run(cmd='set -o pipefail; unset R_HOME && unset R_LIBS &&..._analysis/work/mutect2/1-effects-annotated.vcf.gz', checks=None, log_stdout=False, env=None)
98 error_msg = " ".join(cmd) if not isinstance(cmd, basestring) else cmd
99 error_msg += "\n"
100 error_msg += "".join(debug_stdout)
101 s.communicate()
102 s.stdout.close()
--> 103 raise subprocess.CalledProcessError(exitcode, error_msg)
exitcode = 139
error_msg = 'set -o pipefail; unset R_HOME && unset R_LIBS &&...analysis/work/mutect2/1-effects-annotated.vcf.gz\n'
104 else:
105 break
106 s.communicate()
107 s.stdout.close()
CalledProcessError: Command 'set -o pipefail; unset R_HOME && unset R_LIBS && export PATH=/mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin:$PATH && export TMPDIR=/mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7 && /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
Selected test sample P00132893_tumor and control sample P00132893_normal
/bin/bash: line 1: 65589 Segmentation fault (core dumped) /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
' returned non-zero exit status 139
___________________________________________________________________________
mbeyens@trainingvm-n001:/home/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work$
282
283 def _cnvkit_metrics(cnns, target_bed, antitarget_bed, cov_interval, items):
284 """Estimate noise of a sample using a flat background.
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in run(cmd='unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz', descr='CNVkit segment', data=None, checks=None, region=None, log_error=True, log_stdout=False, env=None)
18 descr = _descr_str(descr, data, region)
19 logger.debug(descr)
20 cmd_id = diagnostics.start_cmd(cmd, descr or "", data)
21 try:
22 logger_cl.debug(" ".join(str(x) for x in cmd) if not isinstance(cmd, basestring) else cmd)
---> 23 _do_run(cmd, checks, log_stdout, env=env)
cmd = 'unset R_HOME && unset R_LIBS && export PATH=/mnt..._analysis/work/mutect2/1-effects-annotated.vcf.gz'
checks = None
log_stdout = False
env = None
24 except:
25 diagnostics.end_cmd(cmd_id, False)
26 if log_error:
27 logger.exception()
...........................................................................
/home/mbeyens/local/share/bcbio/anaconda/lib/python2.7/site-packages/bcbio/provenance/do.py in _do_run(cmd='set -o pipefail; unset R_HOME && unset R_LIBS &&..._analysis/work/mutect2/1-effects-annotated.vcf.gz', checks=None, log_stdout=False, env=None)
98 error_msg = " ".join(cmd) if not isinstance(cmd, basestring) else cmd
99 error_msg += "\n"
100 error_msg += "".join(debug_stdout)
101 s.communicate()
102 s.stdout.close()
--> 103 raise subprocess.CalledProcessError(exitcode, error_msg)
exitcode = 139
error_msg = 'set -o pipefail; unset R_HOME && unset R_LIBS &&...analysis/work/mutect2/1-effects-annotated.vcf.gz\n'
104 else:
105 break
106 s.communicate()
107 s.stdout.close()
CalledProcessError: Command 'set -o pipefail; unset R_HOME && unset R_LIBS && export PATH=/mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin:$PATH && export TMPDIR=/mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7 && /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
Selected test sample P00132893_tumor and control sample P00132893_normal
/bin/bash: line 1: 65589 Segmentation fault (core dumped) /mnt/home_nfs1/mbeyens/local/share/bcbio/anaconda/bin/cnvkit.py segment -p 16 -o /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/bcbiotx/tmpx6HuN7/P00132893_tumor-sort-1.cns /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/structural/P00132893_tumor/cnvkit/raw/P00132893_tumor-sort-1.cnr -v /mnt/home_nfs1/shared_data_core/PNET/10PNET_FF_gitta/raw_data/paired_analysis/work/mutect2/1-effects-annotated.vcf.gz
' returned non-zero exit status 139
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment