A cx_freeze setup script that works for executables for python 3.5+ based apps that use the tkinter graphics framework.

setup.py

"""
A cx_freeze setup script that works for executables for python 3.5+ based
apps that use the tkinter graphics framework.
i.e. fixes the "KeyError: 'TCL_Library'" error
"""
import os
import sys
from cx_Freeze import setup, Executable

# set location tkinter path variables using os
PY_INSTALL_DIR = os.path.dirname(os.path.dirname(os.__file__))
os.environ["TCL_LIBRARY"] = os.path.join(PY_INSTALL_DIR, "tcl", "tcl8.6")
os.environ["TK_LIBRARY"] = os.path.join(PY_INSTALL_DIR, "tcl", "tk8.6")
# dll files required for tkinter apps
TK86TDLL = os.path.join(PY_INSTALL_DIR, "DLLs", "tk86t.dll")
TCL86TDLL = os.path.join(PY_INSTALL_DIR, "DLLs", "tcl86t.dll")

# GUI applications require a different base on Windows
# (the default is for creation of a console application)
BASE = None
if sys.platform == "win32":
    BASE = "Win32GUI"

# Dependencies should be automatically detected, but may need specifying below
OPTIONS = {"build_exe": {"include_files": [TK86TDLL, TCL86TDLL],  # tkinter
                         "packages": ["tkinter"],  # Dependancies here
                         "excludes": [None],  # Excludes here
                        }}

setup(name="test",
      version="0.1",
      description="My GUI application!",
      options=OPTIONS,
      executables=[Executable("test.py", base=BASE)])