ImageFileProblems.py

import PySimpleGUI as sg

layout = [
            [sg.Image(filename=r'C:\Python\PycharmProjects\GooeyGUI\Holiday-Inn-Scheduler-master\imagebad.png')],
            # [sg.Image(filename=r'C:\Python\PycharmProjects\GooeyGUI\Holiday-Inn-Scheduler-master\holidayinnlogo good.png')],
            [sg.Button('Exit')]
         ]

window = sg.Window('My new window', layout)

while True:             # Event Loop
    event, values = window.Read()
    if event in (None, 'Exit'):
        break
    print(event, values)
window.Close()

Results of the 2 runs. First was with good file, the second with the bad file.

"Bad file" (except that GitHub has likely transcoded it

"Good file"