Created
May 19, 2016 14:58
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Short bash for removing duplicates of sequences from a fasta file (keeping one copy of each unique sequence)
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#!bin/bash | |
# remove_duplicate_sequence.shx is a short bash to remove multiple copies of sequences from an input fasta file and saves the result in an output fasta file. | |
# the bash script was based on Pierre Lindenbaum's script: https://www.biostars.org/p/3003/#3008 | |
# input: | |
# -f | --file : fasta file | |
# -o | --output : output file after removing all the sequences | |
# | |
while [ "$1" != "" ]; do | |
case $1 in | |
-f | --file ) shift | |
fileName=$1 | |
;; | |
-o | --output ) shift | |
outputFile=$1 | |
;; | |
* ) exit 1 | |
esac | |
shift | |
done | |
# The following pipline is composed of the following actions: | |
# in every row that starts with '>' put '@' at the end of the row | |
# replace '>' with '#' | |
# remove the break lines, replace '#' with a breakline, replace '@' with a tab | |
# sort the file according to the second column (sequences). the -u option keeps only one copy of each unique string. | |
# add '>' at the begining of each line | |
# sustitute tab (\t) with a breakline (\n) | |
# remove the first line (it's a blank line) and save the result into $output | |
sed '/^>/s/$/@/' < $fileName | | |
sed 's/^>/#/' |\ | |
tr -d '\n' | tr "#" "\n" | tr "@" "\t" |\ | |
sort -u -f -k 2,2 |\ | |
sed -e 's/^/>/' |\ | |
tr '\t' '\n/' |\ | |
tail -n +2 > $outputFile | |
# End of remove_duplicate_sequence.shx |
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Hi, Thanks for the script. I have a quick question. Where I could specify the filename in while loop? Thank you