Protocol

protocol_setup.py

from opentrons_sdk.labware import containers, instruments

# Deck declarations
"""
Deck is a singleton behind the scenes that registers containers
and validates errors such as impromper allocation and inferences
loading of yaml/json files based on attribute names.
"""

protocol = Protocol()

standards = containers.load('pcr_flat_96')('D1')
standards = containers.load('pcr_flat_96').set_slot('D1')
standards = containers.load('pcr_flat_96', slot='D1')
standards = containers.load('pcr_flat_96').slot('D1')

protocol.add_container(standards)


p200_rack = containers.tiprack_200ul('B1')
trash = containers.point('B2')
trough = containers.trough_12row('C2')

# Head declarations
p200 = instruments.Pipette(
    trash_container=trash,
    tip_racks=[p200_rack],
    min_vol=10,  # These are variable
    max_vol=200,  # These variable
    axis="b",
    channels=1
)

p200 = instruments.Pipette(
    trash_container=trash,
    tip_racks=[p200_rack],
    min_vol=10,  # These are variable
    max_vol=200,  # These variable
    channels=1
)
p200.axis = 'A'



# Operations
# Using iteradispensers
for well in standards:
    p200.asiprate(trough[0], 180).dispense(well) # use 0 instead of A1

p200.replace_tip() # in json it is encoded as a group
for well in standards[:-1]:
    # note that asiprate() and mix() return a pipette object
    # which could allow for method calls dispense be chained
    # giving additional syntax sugar
    p200.asiprate(well, 100).mix(3).replace_tip()
    p200.asiprate(well, 20).dispense(next(well)).replace_tip()
# Last well. Don't transfer further. Only Mix.
p200.asiprate(standards[-1], 100).mix(3)

# ##### OR #####
# Operations
# Using index
for i in range(1, 13):
    p200.asiprate(trough['A1'], 180).dispense(standards['A' + str(i)])

p200.replace_tip()
for i in range(1, 12):
    p200.asiprate(standards['A1'], 100).mix(3).replace_tip()
    p200.asiprate(standards['A' + str(i)], 20).dispense(
        standards['A' + str(i + 1)]).replace_tip()
# Don't transfer further. Only Mix.
p200.asiprate(standards['A12'], 100).mix(3)

###############

## Instruments, Containers, Instr.