vignette.ipynb

Dear Dr. Warchal, thanks for the amazing tool! It has been helping me a lot!

As I am relatively new in coding, I wonder if it would be possible to highlight the border of specific wells (based on a separate variable, for exemple). I tried to modify the final plot using common ggplot2 functions, without success. I wonder if you ever had a similar issue.

Thanks a lot in advance for the feedback!

Hi Lucas,

It's possible (but ugly) to to this with ggplot functions.

My idea was to plot squares over the top of the wells with another geom_point() call, and set the stroke conditionally on another column in the dataframe.

library(platetools)
library(ggplot2)

# create example data with 10% wells highlighted
df <- data.frame(
    vals = rnorm(384),
    well = num_to_well(1:384, plate=384),
    highlight = sample(c(TRUE, FALSE), prob=c(0.1, 0.9), 384, replace=TRUE)
)

raw_map(
    data=df$vals,
    well=df$well,
    plate=384,
    size=5
) +
geom_point(
    aes(stroke=df$highlight),
    color="red",
    pch=22,
    size=5
) +
theme_dark()

Dear Dr. Warchal, thank you very much for the reply!
It worked quite nicely for me! I haven't thought about the stroke argument and it was, indeed, a very elegant solution!
Have a nice day!