singularity definition file

macs2.def

BootStrap: docker
From: ubuntu:latest
%post
    apt update && \
    apt install -y \
        git python3 python3-pip
    pip3 install -i https://mirrors.sustech.rocks/pypi/simple MACS2 MACS3

nanopolish_cpggcp.def

BootStrap: docker
From: ubuntu:latest
%environment
    export PATH=/nanopolish:$PATH

%post
    apt update && \
    apt install -y \
        git wget tar zlib1g-dev make g++ python3 python3-pip
    git clone --recursive https://github.com/jts/nanopolish.git
    cd nanopolish
    pip3 install -i https://mirrors.sustech.rocks/pypi/simple -r scripts/requirements.txt
    git checkout nanonome
    git checkout master -- Makefile
    make

sigmap.def

BootStrap: docker
From: ubuntu:latest

%post
    apt update && \
    apt install -y \
               git \
               zlib1g-dev \
               make \
               g++ \

    git clone --recursive https://github.com/haowenz/sigmap.git
    cd sigmap && make
    cp sigmap /usr/local/bin

singularity definition files

# run example
sudo singularity build macs.sif macs2.def