alienzj

Useful Spacemacs commands

• SPC q q - quit
• SPC w / - split window vertically
• SPC w - - split window horizontally
• SPC 1 - switch to window 1
• SPC 2 - switch to window 2
• SPC w c - delete current window
• SPC TAB - switch to previous buffer
• SPC b b - switch buffers

alienzj

#pragma once

#include <boost/noncopyable.hpp>

#include <boost/beast/websocket.hpp>

#include <boost/beast/websocket/ssl.hpp>

struct websocket_stream : boost::noncopyable

alienzj

#!/usr/bin/env bash
# memusg -- Measure memory usage of processes
# Usage: memusg COMMAND [ARGS]...
#
# Author: Jaeho Shin <netj@sparcs.org>
# Created: 2010-08-16
############################################################################
# Copyright 2010 Jaeho Shin.                                               #
#                                                                          #
# Licensed under the Apache License, Version 2.0 (the "License");          #

alienzj

// Sample custom iterator.
// By perfectly.insane (http://www.dreamincode.net/forums/index.php?showuser=76558)
// From: http://www.dreamincode.net/forums/index.php?showtopic=58468

#include <iostream>
#include <vector>
#include <algorithm>
#include <iterator>
#include <cassert>

alienzj

Extracting paired FASTQ read data from a BAM mapping file

Sometimes FASTQ data is aligned to a reference and stored as a BAM file, instead of the normal FASTQ read files. This is okay, because it is possible to recreate raw FASTQ files based on the BAM file. The following outlines this process. The useful software samtools and bedtools are both required.

From each bam, we need to extract:

1. reads that mapped properly as pairs
2. reads that didn’t map properly as pairs (both didn’t map, or one didn’t map)

For #1, the following command will work. This was taken from this webpage.

alienzj

;; -*- mode: emacs-lisp -*-
;; This file is loaded by Spacemacs at startup.
;; It must be stored in your home directory.

(defun dotspacemacs/layers ()
  "Configuration Layers declaration.
You should not put any user code in this function besides modifying the variable
values."
  (setq-default
   ;; Base distribution to use. This is a layer contained in the directory

alienzj

adobe-source-code-pro-fonts
akonadi-calendar-tools
akonadiconsole
akonadi-import-wizard
akregator
alsi
amarok
archlinuxcn-keyring
ark
artikulate

alienzj

setwd("C:\\Users\\xuwenhao\\Desktop\\bwa output\\nwe_bwa\\polu_length_count\\3p_and_5p")
library(ggplot2)
library(gridExtra)
files=list.files(getwd())
files <- files[grepl("w*\\.txt$", files)]
ways=unlist(strsplit(getwd(),"/"))
pdf(paste(ways[length(ways)],".pdf",seq=""),family="GB1")#改后缀

plot_c <- list()
for (f in files)

alienzj

#!/bin/sh
# compute reads length distribution from a fastq file
awk 'NR%4 == 2 {lengths[length($0)]++} END {for (l in lengths) {print l, lengths[l]}}'  file.fastq

alienzj

QTabBar,
QTabBar::tab
{
    font-family: "Noto Sans";
    font-size: 11px;
    height: 16px;
    padding: 2px;
    border: 0px;
    border-bottom: 3px solid palette(dark);
    background-color: palette(dark);