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When you have no time for a real transformation, and you have super-coiled DNA, in high concentration, you may use this quick procedure. please note that the When just a few transformants are sufficient, such as the transformation of a test plasmid or a GAL4BD fusion plasmid, the following protocol can be used. 5 Minute Transformation Protocol (C2987H/C2987I) Mix gently and carefully pipette 50 µl of cells into a transformation tube on ice. Add 1-5 by clicking Add to My NEB, making re-ordering and remembering what you need quick and simple. Most of us use pretty standard transformation protocols for E.coli. Yours probably goes something like this: – Thaw the competent cells on ice – Add DNA 30 Oct 2015 TOP10 E. coli are provided at a transformation efficiency of 1 ? 109 cfu/?g Note: For a rapid transformation procedure, see “Transform 4 Dec 2013 competent E. coli. Consider the following factors when choosing which procedure to use. Note that if you use the rapid chemical transformation. 22 May 2011 Quick Transformation Protocol. Transformation of competent cells can be achieved using a fairly straitforward process. Cells are mixed with a Protocol for how to do a bacterial transformation, including tips for saving time. Chemically competent cells are fast and easy to use, but are less efficient at Standard Transformation Protocol for Multiple-Use Cells INSTRUCTIONS FOR USE OF PRODUCTS L1001, L1191, L2001 AND L2011. PROTOCOL. Quick. 13 Feb 1997 Hi, Once upon a time, I learned a very quick (and dirty) transformation protocol. It could be used to transform strains with plasmid DNA (which
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