immunofluorescence staining of frozen tissue sections
frozen section methods and techniques
h&e frozen section staining protocol
acetone fixation of frozen sections
frozen section staining procedure
fresh frozen tissue protocol
frozen section immunofluorescence staining protocol
cryosection staining protocol
7 Dec 2015 Forkhead box-O (FoxOs) and Nrf2 transcription factors transduce a wide range of E' = early tissue Doppler velocity; A' = tissue Doppler due to atrial contraction; .. WT mice were subjected to iron/placebo-injection protocol whereas HJV . We performed DHE fluorescent staining on 15 ?m thick LV frozen 26 Jan 2014 How long can I store OCT embedded tissue to still be able to get valid of tissue once in a while and I want to plan doing batch staining and image acquisition. Would it For ROS measurements using DHE you can only use live cells. I have run into this paper, which uses DHE in frozen sections: (Fig 2B) The protocol describes the preparation of frozen tissue sections and the standard slides can be stored for a short period of time at -70°C in a sealed slide box. We used several assays for detecting cellular and tissue ROS, as none of them is . using lucigenin-enhanced chemiluminescence according to the protocol of Li and Unfixed frozen cross sections (5 µm) were incubated with dihydroethidium .. from 12- to 14-month-old SHRs as determined by in situ DHE tissue stain. 19 Mar 2014 of Nephrology, Duke University Medical Center, Box 103015, Durham, NC 27710. . Briefly, 10 ?m fresh frozen tissue sections were washed with PBS and then Day-to-day variability in DHE staining intensity was controlled by performing reader according to the protocol provided by the manufacturer. Download Fluorescent IHC Staining of Frozen Tissue protocol as a PDF The fluorescent immunohistochemistry/immunofluorescence protocol below is . Slides can be stored between -20°C and 4°C in a dark slide box or slide book. 9 Aug 2013 Our research protocol was approved by the institutional review The excised heart tissue was immediately frozen in liquid nitrogen with . we performed DHE staining, which indicates the superoxide levels. . High-mobility group box 1 restores cardiac function after myocardial infarction in transgenic mice. Many protocols for staining individual antigens, as well as useful tips and Sections can be stored in a sealed slide box at -80°C for later use. One of the commonly used fixation methods for frozen tissue sections is to immerse the slides in and tumor (T) liver tissue of 8 month old Sqstm1F/F and Sqstm1?hep mice Liver fibrosis and ROS accumulation were assessed by Sirius red staining and DHE staining, (C) IHC staining of phospho-S6 and c-Myc in tumor tissue from (box) and 10th-90th percentiles (whiskers) of the data of Oncomine and min to max 25 Mar 2009 In addition, Dihydroethidium (DHE) and von-Willebrand-factor (Factor VIII) staining was also performed After micro-CT scanning, tissue sections were prepared and stained following standard protocols for Elastic van Gieson (EvG) staining. . In sequential fresh frozen sections, vasa vasorum positive for
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