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GSE22631 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=gse22631). Only partial data is put here.
ExpId title reference_ch1 time point_ch2 replicate_ch2 agent_ch2 description
GSM561077 F11_FSK2h_rep1 F11 cells, no forskolin 2hrs 1 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561078 F11_FSK2h_rep2 F11 cells, no forskolin 2hrs 2 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561079 F11_FSK2h_rep3 F11 cells, no forskolin 2hrs 3 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561080 F11_FSK4h_rep1 F11 cells, no forskolin 4hrs 1 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561081 F11_FSK4h_rep2 F11 cells, no forskolin 4hrs 2 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561082 F11_FSK4h_rep3 F11 cells, no forskolin 4hrs 3 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561083 F11_FSK24h_rep1 F11 cells, no forskolin 24hrs 1 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561084 F11_FSK24h_rep2 F11 cells, no forskolin 24hrs 2 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561085 F11_FSK24h_rep3 F11 cells, no forskolin 24hrs 3 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561086 F11_FSK48h_rep1 F11 cells, no forskolin 48hrs 1 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561087 F11_FSK48h_rep2 F11 cells, no forskolin 48hrs 2 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
GSM561088 F11_FSK48h_rep3 F11 cells, no forskolin 48hrs 3 10µM forskolin gene expression data of regenerating dorsal root ganglion neurons
^DATABASE = GeoMiame
!Database_name = Gene Expression Omnibus (GEO)
!Database_institute = NCBI NLM NIH
!Database_web_link = http://www.ncbi.nlm.nih.gov/geo
!Database_email = geo@ncbi.nlm.nih.gov
^SERIES = GSE22631
!Series_title = Whole-genome gene expression profiling of F11 dorsal root ganglion (DRG)-like cells stimulated with forskolin
!Series_geo_accession = GSE22631
!Series_status = Public on Jul 01 2010
!Series_submission_date = Jun 30 2010
!Series_last_update_date = Jan 09 2014
!Series_pubmed_id = 21112399
!Series_summary = We generated whole-genome gene expression profiles of F11 dorsal root ganglion (DRG)-like neuroblastoma cells stimulated with the adenylate cyclase activator forskolin for 0, 2, 4, 24 and 48 hours.
!Series_overall_design = F11 neuroblastoma cells were incubated in low-serum medium (DMEM with 0.5% FCS and antibiotics) for three hours and then stimulated with 10 µM forskolin for 2, 4, 24 and 48 hours. All samples are in triplicate. F11 cells incubated in low-serum medium for three hours, but not stimulated with forskolin, were used as a common reference.
!Series_type = Expression profiling by array
!Series_contributor = Harold,D,MacGillavry
!Series_contributor = Jochem,,Cornelis
!Series_contributor = Joost,,Verhaagen
!Series_contributor = August,B,Smit
!Series_contributor = Ronald,E,van Kesteren
!Series_sample_id = GSM561077
!Series_sample_id = GSM561078
!Series_sample_id = GSM561079
!Series_sample_id = GSM561080
!Series_sample_id = GSM561081
!Series_sample_id = GSM561082
!Series_sample_id = GSM561083
!Series_sample_id = GSM561084
!Series_sample_id = GSM561085
!Series_sample_id = GSM561086
!Series_sample_id = GSM561087
!Series_sample_id = GSM561088
!Series_contact_name = Harold,D,MacGillavry
!Series_contact_email = hmacgillavry@gmail.com
!Series_contact_laboratory = Molecular and cellular neurobiology
!Series_contact_department = Center for neurogenomics and cognitive research
!Series_contact_institute = Vrije Universiteit
!Series_contact_address = De Boelelaan 1087
!Series_contact_city = Amsterdam
!Series_contact_zip/postal_code = 1081HV
!Series_contact_country = Netherlands
!Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/series/GSE22631/GSE22631_RAW.tar
!Series_platform_id = GPL4135
!Series_platform_taxid = 10116
!Series_sample_taxid = 10116
!Series_relation = BioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA128185
^PLATFORM = GPL4135
!Platform_title = Agilent-014879 Whole Rat Genome Microarray 4x44K G4131F (Feature Number version)
!Platform_geo_accession = GPL4135
!Platform_status = Public on Aug 17 2006
!Platform_submission_date = Aug 17 2006
!Platform_last_update_date = Jan 09 2014
!Platform_technology = in situ oligonucleotide
!Platform_distribution = custom-commercial
!Platform_organism = Rattus norvegicus
!Platform_taxid = 10116
!Platform_manufacturer = Agilent Technologies
!Platform_manufacture_protocol = see manufacturer's web site at http://www.agilent.com/
!Platform_catalog_number = G4131F
!Platform_description =
!Platform_description = With a focus on well known rat genes and homologues to human and mouse genes with useful annotation, Agilent's Whole Rat Genome Oligo – 4X44K microarray provides researchers with a new tool for modeling human biology in the rat model organism. For researchers, this means they now have access to a microarray made up of relevant content that has been empirically validated by Agilent.
!Platform_description =
!Platform_description = Arrays of this design have barcodes that begin with 16014879 or 2514879.
!Platform_description =
!Platform_description = Orientation:
!Platform_description = Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software.
!Platform_description =
!Platform_description = The ID column represents the Agilent Feature Extraction feature number.
!Platform_description =
!Platform_description = Rows and columns are numbered as scanned by an Axon Scanner (barcode on the bottom, DNA on the front surface).
!Platform_description =
!Platform_description = To match data scanned on an Axon scanner, use the RefNumber column contained in the Agilent-provided GAL file as the ID_REF column in sample submissions.
!Platform_description =
!Platform_description = *** A different version of this platform with the Agilent Probe names in the ID column is assigned accession number GPL7294.
!Platform_contact_name = ,,Agilent Technologies
!Platform_contact_email = cag_sales-na@agilent.com
!Platform_contact_phone = 877-424-4536
!Platform_contact_department =
!Platform_contact_institute = Agilent Technologies
!Platform_contact_address =
!Platform_contact_city = Palo Alto
!Platform_contact_state = CA
!Platform_contact_zip/postal_code = 94304
!Platform_contact_country = USA
!Platform_contact_web_link = www.agilent.com
!Platform_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/platforms/GPL4135/GPL4135_old_annotations.txt.gz
!Platform_relation = Alternative to: GPL7294
!Platform_data_row_count = 45220
#ID = Agilent feature number
#COL = Column
#ROW = Row
#NAME = Name
#SPOT_ID = Spot identifier
#CONTROL_TYPE = Control type
#REFSEQ = RefSeqAccession
#GB_ACC = GenBankAccession LINK_PRE:"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&term="
#GENE = Entrez Gene ID LINK_PRE:"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids="
#GENE_SYMBOL = Gene Symbol
#GENE_NAME = Gene Name
#UNIGENE_ID = UnigeneID
#ENSEMBL_ID = EnsemblID
#TIGR_ID = TIGRID
#ACCESSION_STRING = Accession String
#CHROMOSOMAL_LOCATION = Chromosomal Location
#CYTOBAND = Cytoband
#DESCRIPTION = Description
#GO_ID = GoIDs
#SEQUENCE =
!platform_table_begin
ID COL ROW NAME SPOT_ID CONTROL_TYPE REFSEQ GB_ACC GENE GENE_SYMBOL GENE_NAME UNIGENE_ID ENSEMBL_ID TIGR_ID ACCESSION_STRING CHROMOSOMAL_LOCATION CYTOBAND DESCRIPTION GO_ID SEQUENCE
1 266 170 GE_BrightCorner GE_BrightCorner pos
2 266 168 DarkCorner DarkCorner pos
3 266 166 DarkCorner DarkCorner pos
4 266 164 DarkCorner DarkCorner pos
5 266 162 DarkCorner DarkCorner pos
6 266 160 DarkCorner DarkCorner pos
7 266 158 DarkCorner DarkCorner pos
8 266 156 DarkCorner DarkCorner pos
9 266 154 DarkCorner DarkCorner pos
10 266 152 DarkCorner DarkCorner pos
11 266 150 DarkCorner DarkCorner pos
12 266 148 AA892298 A_44_P465448 FALSE AA892298 Rn.14747 gb|AA892298 chr9:57247899-57249303 rn|9q31 AA892298 EST196101 Normalized rat kidney, Bento Soares Rattus sp. cDNA clone RKIAO74 3' end, mRNA sequence [AA892298] CCACATCTGTATGCAGTGTCACAGACATTCTCCTTCTTGATGCTTCTAGGGAGCACTATT
13 266 146 AI232741 A_44_P514796 FALSE AI232741 Rn.12611 gb|AI232741|gb|AW254068|gb|BI278122 chr7:122235251-122235192 rn|7q34 AI232741 EST229429 Normalized rat kidney, Bento Soares Rattus sp. cDNA clone RKICF89 3' end, mRNA sequence [AI232741] GTTTAAAATTTATTGACGTGATTTCCCATAAGGATGACGGCCCAGAAGCACTCAGCTGTG
14 266 144 NM_057188 A_44_P409518 FALSE NM_057188 NM_057188 117533 Gmpr guanosine monophosphate reductase Rn.3862 ENSRNOT00000023613 TC568850 ref|NM_057188|gb|AF090867|ens|ENSRNOT00000023613|tc|TC568850 chr17:25153992-25153933 rn|17p13 Rattus norvegicus guanosine monophosphate reductase (Gmpr), mRNA [NM_057188] GO:0003824(catalytic activity)|GO:0003920(GMP reductase activity)|GO:0009409(response to cold)|GO:0016491(oxidoreductase activity)|GO:0030955(potassium ion binding)|GO:0046872(metal ion binding) GCAGAAGCTGAAGCTTTTCTACGGCATGAGCTCAGACACAGCCATGAAGAAACACGCGGG
15 266 142 XM_236342 A_44_P279262 FALSE XM_236342 Rn.21843 ENSRNOT00000049602 gb|XM_236342|ens|ENSRNOT00000049602 chr8:69116608-69116667 rn|8q24 Rattus norvegicus similar to CRAG protein (4C711) (LOC315756), mRNA [XM_236342] TTGGGTTTAACGTCTCTGGTAGATGGAAAGTCTGATGGTTCTAGAACGATTCACACTAAC
16 266 140 NM_001014243 A_44_P375042 FALSE NM_001014243 NM_001014243 365215 RGD1309888 similar to RIKEN cDNA 1500002O20 Rn.32677 ENSRNOT00000026669 TC521750 ref|NM_001014243|gb|XM_344869|gb|BC087051|ens|ENSRNOT00000026669 chr1:79742705-79743531 rn|1q21 Rattus norvegicus similar to RIKEN cDNA 1500002O20 (RGD1309888), mRNA [NM_001014243] CCACAGCTGTCGCACACAATCCTCACGGAGAAGAACTGGTTCCACTATGCTGCCAGAATC
17 266 138 XM_222163 A_44_P269499 FALSE XM_222163 Rn.154560 ENSRNOT00000011278 gb|XM_222163|gb|XR_009247|ens|ENSRNOT00000011278 chr12:30395960-30395901 rn|12q14 Rattus norvegicus similar to 17,000 dalton myosin light chain (LOC304447), mRNA [XM_222163] TGAAATCCGTCATATCCTAGTCACGCTGGGCGAGAATATGACAGAGGAAGAAGTAGAGAT
18 266 136 AA891661 A_44_P204808 FALSE AA891661 Rn.1618 gb|AA891661 chr4:84396575-84396516 rn|4q24 AA891661 EST195464 Normalized rat kidney, Bento Soares Rattus sp. cDNA clone RKIAF23 3' end, mRNA sequence [AA891661] TATTCTGCTGCTTCTAAGCACCTGAAGAATGTGGCTCTCGGTTCACGACATGGTTAACGG
19 266 134 NM_033350 A_44_P438090 FALSE NM_033350 NM_033350 29322 Plcb3 phospholipase C, beta 3 Rn.16983 ENSRNOT00000028720 TC538381 ref|NM_033350|gb|XM_342005|gb|M99567|ens|ENSRNOT00000028720 chr1:209787835-209787776 rn|1q43 Rattus norvegicus phospholipase C, beta 3 (Plcb3), mRNA [NM_033350] GO:0004629(phospholipase C activity)|GO:0004871(signal transducer activity)|GO:0007242(intracellular signaling cascade)|GO:0016042(lipid catabolism)|GO:0016787(hydrolase activity) GGCTCAGGGAGGTTGTCCTGGACGCACATACGACTCAGTTCAAGAGGCTGAAGGAGTTGA
20 266 132 XM_214021 A_44_P330643 FALSE XM_214021 XM_214021 289561 Polr2b_predicted polymerase (RNA) II (DNA directed) polypeptide B (predicted) Rn.153952 ENSRNOT00000039252 TC535070 ref|XM_214021|ref|XM_001075841|gb|CB546931|ens|ENSRNOT00000039252 chr14:33077593-33077330 rn|14p11 PREDICTED: Rattus norvegicus polymerase (RNA) II (DNA directed) polypeptide B (predicted) (Polr2b_predicted), mRNA [XM_214021] GO:0003677(DNA binding)|GO:0005665(DNA-directed RNA polymerase II, core complex)|GO:0006366(transcription from RNA polymerase II promoter) AGAGGACATGCCGTTCACTTGTGAAGGCATAACTCCTGACATCATCATAAACCCCCACGC
...
45208 1 25 XM_001081752 A_44_P1070720 FALSE XM_001081752 XM_001081752 360667 RGD1307394_predicted similar to hypothetical protein ET (predicted) Rn.163173 TC517793 ref|XM_001081752|gb|AW253635|gb|AA955448|gb|AW252841 chr10:106986790-106986849 rn|10q32.3 PREDICTED: Rattus norvegicus similar to hypothetical protein ET (predicted) (RGD1307394_predicted), mRNA [XM_001081752] AGCAGTTGCCACTCTGTGTGGATCCGGAAATGACTCCAACACATATCAGGGATGCTAAGA
45209 1 23 AA891475 A_44_P596890 FALSE AA891475 Rn.3456 gb|AA891475|gb|BQ780386 chr12_random:590345-590404 EST195278 Normalized rat heart, Bento Soares Rattus sp. cDNA clone RHEAU83 3' end, mRNA sequence [AA891475] ACCACAACTTTGGCCAAGACCATCCCAACCATAGCTCCCAGGTTCAGCAGTGAAGGATTC
45210 1 21 DarkCorner DarkCorner pos
45211 1 19 DarkCorner DarkCorner pos
45212 1 17 DarkCorner DarkCorner pos
45213 1 15 DarkCorner DarkCorner pos
45214 1 13 DarkCorner DarkCorner pos
45215 1 11 DarkCorner DarkCorner pos
45216 1 9 DarkCorner DarkCorner pos
45217 1 7 DarkCorner DarkCorner pos
45218 1 5 DarkCorner DarkCorner pos
45219 1 3 GE_BrightCorner GE_BrightCorner pos
45220 1 1 GE_BrightCorner GE_BrightCorner pos
!platform_table_end
^SAMPLE = GSM561077
!Sample_title = F11_FSK2h_rep1
!Sample_geo_accession = GSM561077
!Sample_status = Public on Jul 01 2010
!Sample_submission_date = Jun 30 2010
!Sample_last_update_date = Jun 30 2010
!Sample_type = RNA
!Sample_channel_count = 2
!Sample_source_name_ch1 = F11 neuroblastoma cells
!Sample_organism_ch1 = Rattus norvegicus
!Sample_taxid_ch1 = 10116
!Sample_characteristics_ch1 = reference: F11 cells, no forskolin
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = Total RNA was extracted using Trizol following manufacturer's instructions
!Sample_label_ch1 = Cy5
!Sample_label_protocol_ch1 = Agilent labeling kit, according to manufacturers instructions.
!Sample_source_name_ch2 = F11 neuroblastoma cells
!Sample_organism_ch2 = Rattus norvegicus
!Sample_taxid_ch2 = 10116
!Sample_characteristics_ch2 = time point: 2hrs
!Sample_characteristics_ch2 = replicate: 1
!Sample_characteristics_ch2 = agent: 10µM forskolin
!Sample_molecule_ch2 = total RNA
!Sample_extract_protocol_ch2 = Total RNA was extracted using Trizol following manufacturer's instructions
!Sample_label_ch2 = Cy3
!Sample_label_protocol_ch2 = Agilent labeling kit, according to manufacturers instructions.
!Sample_hyb_protocol = According to manufacturers instructions.
!Sample_scan_protocol = Agilent scanner
!Sample_description = gene expression data of regenerating dorsal root ganglion neurons
!Sample_data_processing = The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays.
!Sample_platform_id = GPL4135
!Sample_contact_name = Harold,D,MacGillavry
!Sample_contact_email = hmacgillavry@gmail.com
!Sample_contact_laboratory = Molecular and cellular neurobiology
!Sample_contact_department = Center for neurogenomics and cognitive research
!Sample_contact_institute = Vrije Universiteit
!Sample_contact_address = De Boelelaan 1087
!Sample_contact_city = Amsterdam
!Sample_contact_zip/postal_code = 1081HV
!Sample_contact_country = Netherlands
!Sample_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561077/GSM561077.txt.gz
!Sample_series_id = GSE22631
!Sample_data_row_count = 43209
#ID_REF =
#VALUE = Values represent normalized log fold changes relative to the common reference sample.
!sample_table_begin
ID_REF VALUE
12 -0.252883408
13 0.080360756
14 -0.002192043
15 -0.277088678
16 -0.118405747
17 -0.304502048
18 0.048163755
19 -0.116972778
20 -0.382371486
...
45005 -0.049426219
45006 -0.125253972
45007 -0.142154606
!sample_table_end
!Series_title "Whole-genome gene expression profiling of F11 dorsal root ganglion (DRG)-like cells stimulated with forskolin"
!Series_geo_accession "GSE22631"
!Series_status "Public on Jul 01 2010"
!Series_submission_date "Jun 30 2010"
!Series_last_update_date "Jan 09 2014"
!Series_pubmed_id "21112399"
!Series_summary "We generated whole-genome gene expression profiles of F11 dorsal root ganglion (DRG)-like neuroblastoma cells stimulated with the adenylate cyclase activator forskolin for 0, 2, 4, 24 and 48 hours."
!Series_overall_design "F11 neuroblastoma cells were incubated in low-serum medium (DMEM with 0.5% FCS and antibiotics) for three hours and then stimulated with 10 µM forskolin for 2, 4, 24 and 48 hours. All samples are in triplicate. F11 cells incubated in low-serum medium for three hours, but not stimulated with forskolin, were used as a common reference."
!Series_type "Expression profiling by array"
!Series_contributor "Harold,D,MacGillavry"
!Series_contributor "Jochem,,Cornelis"
!Series_contributor "Joost,,Verhaagen"
!Series_contributor "August,B,Smit"
!Series_contributor "Ronald,E,van Kesteren"
!Series_sample_id "GSM561077 GSM561078 GSM561079 GSM561080 GSM561081 GSM561082 GSM561083 GSM561084 GSM561085 GSM561086 GSM561087 GSM561088 "
!Series_contact_name "Harold,D,MacGillavry"
!Series_contact_email "hmacgillavry@gmail.com"
!Series_contact_laboratory "Molecular and cellular neurobiology"
!Series_contact_department "Center for neurogenomics and cognitive research"
!Series_contact_institute "Vrije Universiteit"
!Series_contact_address "De Boelelaan 1087"
!Series_contact_city "Amsterdam"
!Series_contact_zip/postal_code "1081HV"
!Series_contact_country "Netherlands"
!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/series/GSE22631/GSE22631_RAW.tar"
!Series_platform_id "GPL4135"
!Series_platform_taxid "10116"
!Series_sample_taxid "10116"
!Series_relation "BioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA128185"
!Sample_title "F11_FSK2h_rep1" "F11_FSK2h_rep2" "F11_FSK2h_rep3" "F11_FSK4h_rep1" "F11_FSK4h_rep2" "F11_FSK4h_rep3" "F11_FSK24h_rep1" "F11_FSK24h_rep2" "F11_FSK24h_rep3" "F11_FSK48h_rep1" "F11_FSK48h_rep2" "F11_FSK48h_rep3"
!Sample_geo_accession "GSM561077" "GSM561078" "GSM561079" "GSM561080" "GSM561081" "GSM561082" "GSM561083" "GSM561084" "GSM561085" "GSM561086" "GSM561087" "GSM561088"
!Sample_status "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010" "Public on Jul 01 2010"
!Sample_submission_date "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010"
!Sample_last_update_date "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010" "Jun 30 2010"
!Sample_type "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" "RNA"
!Sample_channel_count "2" "2" "2" "2" "2" "2" "2" "2" "2" "2" "2" "2"
!Sample_source_name_ch1 "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells"
!Sample_organism_ch1 "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus"
!Sample_characteristics_ch1 "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin" "reference: F11 cells, no forskolin"
!Sample_molecule_ch1 "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA"
!Sample_extract_protocol_ch1 "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions"
!Sample_label_ch1 "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5" "Cy5"
!Sample_label_protocol_ch1 "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions."
!Sample_taxid_ch1 "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116"
!Sample_source_name_ch2 "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells" "F11 neuroblastoma cells"
!Sample_organism_ch2 "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus" "Rattus norvegicus"
!Sample_characteristics_ch2 "time point: 2hrs" "time point: 2hrs" "time point: 2hrs" "time point: 4hrs" "time point: 4hrs" "time point: 4hrs" "time point: 24hrs" "time point: 24hrs" "time point: 24hrs" "time point: 48hrs" "time point: 48hrs" "time point: 48hrs"
!Sample_characteristics_ch2 "replicate: 1" "replicate: 2" "replicate: 3" "replicate: 1" "replicate: 2" "replicate: 3" "replicate: 1" "replicate: 2" "replicate: 3" "replicate: 1" "replicate: 2" "replicate: 3"
!Sample_characteristics_ch2 "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin" "agent: 10µM forskolin"
!Sample_molecule_ch2 "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA"
!Sample_extract_protocol_ch2 "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions" "Total RNA was extracted using Trizol following manufacturer's instructions"
!Sample_label_ch2 "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3" "Cy3"
!Sample_label_protocol_ch2 "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions." "Agilent labeling kit, according to manufacturers instructions."
!Sample_taxid_ch2 "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116" "10116"
!Sample_hyb_protocol "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions." "According to manufacturers instructions."
!Sample_scan_protocol "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner" "Agilent scanner"
!Sample_description "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons" "gene expression data of regenerating dorsal root ganglion neurons"
!Sample_data_processing "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays." "The data were analyzed with the R packages 'bioconductor' and 'limma'. Raw spot intensities were background corrected using Edwards method and loess normalized within arrays."
!Sample_platform_id "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135" "GPL4135"
!Sample_contact_name "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry" "Harold,D,MacGillavry"
!Sample_contact_email "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com" "hmacgillavry@gmail.com"
!Sample_contact_laboratory "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology" "Molecular and cellular neurobiology"
!Sample_contact_department "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research" "Center for neurogenomics and cognitive research"
!Sample_contact_institute "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit" "Vrije Universiteit"
!Sample_contact_address "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087" "De Boelelaan 1087"
!Sample_contact_city "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam" "Amsterdam"
!Sample_contact_zip/postal_code "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV" "1081HV"
!Sample_contact_country "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands" "Netherlands"
!Sample_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561077/GSM561077.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561078/GSM561078.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561079/GSM561079.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561080/GSM561080.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561081/GSM561081.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561082/GSM561082.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561083/GSM561083.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561084/GSM561084.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561085/GSM561085.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561086/GSM561086.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561087/GSM561087.txt.gz" "ftp://ftp.ncbi.nlm.nih.gov/pub/geo/DATA/supplementary/samples/GSM561nnn/GSM561088/GSM561088.txt.gz"
!Sample_data_row_count "43209" "43209" "43209" "43209" "43209" "43209" "43209" "43209" "43209" "43209" "43209" "43209"
!series_matrix_table_begin
"ID_REF" "GSM561077" "GSM561078" "GSM561079" "GSM561080" "GSM561081" "GSM561082" "GSM561083" "GSM561084" "GSM561085" "GSM561086" "GSM561087" "GSM561088"
12 -0.252883408 -0.0247087 -0.317303585 -0.197493613 -0.116559833 -0.018594176 -0.299225031 -0.03392886 -0.097087237 -0.312433122 -0.006008374 0.054344992
13 0.080360756 0.020169715 -0.068080323 0.040796871 -0.035966923 0.155397512 0.225838787 0.117886361 0.08047195 0.21598567 0.195152231 0.210199191
14 -0.002192043 -0.068309688 -0.235050025 -0.117271781 -0.044618351 0.0837161 -0.332804548 -0.666437384 -0.615476634 -0.474878949 -0.453368353 -0.32045835
15 -0.277088678 -0.066819304 -0.321728938 -0.038420125 -0.13220134 -0.164610438 -0.253768487 -0.122552263 -0.283580881 0.165329687 -0.174798057 -0.294074313
16 -0.118405747 -0.273390894 -0.211553406 -0.281463935 -0.147628634 -0.274623502 0.128872991 0.171521064 -0.046190812 0.369457744 0.412627942 0.184190845
17 -0.304502048 -0.055685553 0.015899364 -0.159971316 -0.065692305 -0.086884557 -0.506944497 -0.295889251 -0.47656207 -0.24615346 0.08636249 0.005428025
18 0.048163755 -0.231968686 -0.14253321 -0.116844655 0.096544948 -0.112055976 -0.134796753 -0.010570385 -0.323041671 -0.22350106 -0.384308772 -0.229741218
19 -0.116972778 -0.420979996 -0.304877711 -0.160118476 0.106946623 -0.176322792 -0.146286307 -0.0145322 -0.310002206 -0.306877205 -0.136526986 -0.40355176
20 -0.382371486 -0.038337602 -0.137603442 -0.368404916 -0.26789179 -0.135886877 -0.050661652 0.136312597 -0.08362125 -0.178088482 0.116385937 0.364631317
...
45000 -0.24247638 -0.088001763 -0.19772851 -0.145069457 -0.208632731 -0.185178148 0.184233447 0.431595202 0.444474992 0.345786071 0.364846527 0.497243659
45001 -0.458856895 -0.57542884 -0.660674312 -0.472087373 -0.479579004 -0.418470689 -0.049230357 -0.165502236 -0.129864263 0.075828206 -0.187886846 -0.03788123
45002 -0.269935086 -0.376064718 -0.361127646 -0.096076238 -0.01903622 -0.218935118 -0.005300131 -0.215026447 -0.257423159 -0.051965864 -0.215086644 -0.177496745
45003 -0.041966147 0.093737235 0.093043861 -0.205403796 0.126754809 0.067467969 -0.824960334 -0.757000433 -0.739648339 -0.497927713 -0.382460841 -0.4362647
45004 -0.438138971 -0.180587778 -0.332052241 -0.062393794 -0.140670196 -0.314409674 -0.147531967 -0.096596913 0.007690412 -0.165425029 -0.296957026 -0.256891886
45005 0.019717304 -0.068851267 0.019235094 0.045413845 -0.112623223 -0.015728849 -0.130484016 -0.168444737 0.01956729 -0.172041929 -0.248411333 -0.049426219
45006 -0.431103857 -0.38594631 -0.092585966 -0.212157806 -0.107011213 -0.071012688 -0.177460891 -0.203117415 -0.395944459 -0.088941122 -0.422438588 -0.125253972
45007 -0.186344003 -0.327897979 -0.202163537 -0.072919728 0.013938671 -0.151988402 -0.157577036 -0.141525525 -0.130990766 -0.013390397 -0.161744478 -0.142154606
!series_matrix_table_end
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