Created
March 31, 2016 12:01
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#python | |
# a script that makes a haploid vcf diploid. Expects a whole genome VCF | |
import gzip | |
import csv | |
import argparse | |
import sys | |
parser = argparse.ArgumentParser(description="A script that makes a haploid vcf diploid. Expects a whole genome VCF.") | |
parser.add_argument("-v", "--vcf", action="store", required=True, help="Input VCF file. Should be a multisample, whole genome VCF from Shore with haploid calls.") | |
parser.add_argument("-o", "--out", action="store", required=True, help="Output filename") | |
parser.add_argument("-g", "--gzip", action="store_true", required=False, help="Set if the VCF is gzipped.") | |
args = parser.parse_args() | |
vcf_in = args.vcf | |
out_name = args.out | |
if args.gzip: | |
opener = gzip.open | |
else: | |
opener = open | |
with opener(vcf_in, 'r') as tsvin: | |
tsvin = csv.reader(tsvin, delimiter='\t') | |
vcf_out = csv.writer(open(out_name, 'w'), delimiter='\t', lineterminator="\n") | |
for row in tsvin: | |
if any('##' in strings for strings in row): | |
vcf_out.writerow(row) | |
continue | |
if any('#CHROM' in strings for strings in row): | |
vcf_out.writerow(row) | |
continue | |
chrom,pos,id,ref,alt,qual,filter,info,format=row[0:9] | |
out = [chrom,pos,id,ref,alt,qual,filter,info,format] | |
haplotypes = row[9:] | |
for hap in haplotypes: | |
call = hap.split(":")[0] # get the 0 | |
fixed_hap = call+"|"+call+":"+hap.split(":")[1]+":"+hap.split(":")[2] | |
out.append(fixed_hap) | |
vcf_out.writerow(out) |
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