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November 8, 2015 20:09
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Make an SFS from an MS output file
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#!/bin/python | |
#xkcd SFS plots | |
# usage: ms 20 10000 -t 50 -r 10 1000 > neutral_ms.txt; python XKCD_sfs_plots.py neutral_ms.txt | |
from matplotlib import pyplot as plt | |
import numpy as np | |
from itertools import groupby | |
from sys import argv | |
ms_file = open(argv[1]) | |
ms_list = list(ms_file) | |
ms_list2 = [list(group) for k, group in groupby(ms_list, lambda x: x == "//\n") if not k] | |
final_sfs = [] | |
for rep, sim in enumerate(ms_list2): | |
positions = [] | |
snpmatrix = [] | |
if any("ms" in s for s in sim): | |
continue | |
positions = [s for s in sim if "positions" in s] # grab the positions line from the output | |
start_mat = sim.index(positions[0]) | |
snpmatrix = sim[start_mat+1:] | |
snpmatrix.pop(-1) | |
snpmatrix = [list(s.strip()) for s in snpmatrix] | |
snpmatrix = zip(*snpmatrix) | |
sfs = np.zeros(len(snpmatrix[0])) | |
for pos in snpmatrix: | |
num_mutations = pos.count('1') | |
if num_mutations == 0: | |
continue | |
sfs[num_mutations-1] = sfs[num_mutations-1]+1 | |
final_sfs.append(sfs) | |
sfs_lengths = [len(x) for x in final_sfs] | |
data = np.zeros(max(sfs_lengths)) | |
for sfs in final_sfs: | |
if len(sfs) < len(data): | |
size_diff = len(data) - len(sfs) | |
sfs = np.lib.pad(sfs, (0,size_diff), "constant", constant_values=0) | |
data = np.add(data, sfs) | |
if data[-1] == 0: | |
data = np.delete(data, -1) | |
data = np.insert(data, 0, 0) | |
plt.xkcd() | |
fig = plt.figure() | |
ax = fig.add_subplot(1, 1, 1) | |
ax.spines['right'].set_color('none') | |
ax.spines['top'].set_color('none') | |
ax.xaxis.set_ticks_position('bottom') | |
plt.xticks(xrange(1, len(data))) | |
plt.yticks([]) | |
ax.set_xlim([1, len(data)]) | |
ax.set_ylim([0, np.amax(data)]) | |
plt.plot(data, 'r-.') | |
plt.title("SITE FREQUENCY SPECTRUM") | |
plt.xlabel('NUMBER OF MUTATIONS') | |
plt.ylabel('FREQUENCY') | |
plt.show() |
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