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cathalgarvey/RestrictionEnzymes.json

Created July 12, 2012 09:39
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A somewhat exhaustive collection of restriction enzymes in JSON format.
Raw
RestrictionEnzymes.json
This file has been truncated, but you can view the full file.
{
"binsi": {
"target_site": "CCWGG",
"name": "BinSI",
"suppliers": [],
"source": "ATCC 15702",
"references": [
"Khosaka, T., Kiwaki, M., Rak, B., (1983) FEBS Lett., vol. 163, pp. 170-174."
],
"prototype": "EcoRII",
"organism": "Bifidobacterium infantis S76e",
"methylation_site": ""
},
"sthhi": {
"target_site": "GGTACC",
"name": "SthHI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY224",
"methylation_site": ""
},
"eaei": {
"target_site": "Y^GGCCR",
"name": "EaeI",
"suppliers": [
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)"
],
"source": "P.R. Whitehead",
"references": [
"Jacobs, D., Brown, N.L., (1986) Biochem. J., vol. 238, pp. 613-616.",
"Lee, K.-F., Shaw, P.-C., Picone, S.J., Wilson, G.G., Lunnen, K.D., (1998) Biol. Chem., vol. 379, pp. 437-441.",
"Whitehead, P.R., Brown, N.L., (1983) FEBS Lett., vol. 155, pp. 97-101."
],
"prototype": "CfrI",
"organism": "Enterobacter aerogenes",
"methylation_site": "4(5)"
},
"hpych4ii": {
"target_site": "CTNAG",
"name": "HpyCH4II",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori CH4",
"methylation_site": ""
},
"nsphii": {
"target_site": "GGWCC",
"name": "NspHII",
"suppliers": [],
"source": "ATCC 29106",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaII",
"organism": "Nostoc species",
"methylation_site": ""
},
"psc45i": {
"target_site": "?",
"name": "Psc45I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species 45",
"methylation_site": ""
},
"frioi": {
"target_site": "GRGCY^C",
"name": "FriOI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Belichenko, O.A., Prikhodko, E.A., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Flavobacterium species O9",
"methylation_site": ""
},
"bsp145i": {
"target_site": "ATCGAT",
"name": "Bsp145I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species cy145",
"methylation_site": ""
},
"asiai": {
"target_site": "A^CCGGT",
"name": "AsiAI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Bondar, T.S., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AgeI",
"organism": "Arthrobacter species A7359",
"methylation_site": ""
},
"btei": {
"target_site": "GG^CC",
"name": "BteI",
"suppliers": [],
"source": "CAMB 2590",
"references": [
"Mushtaq, R., Sohail, A., Malik, K., Riazuddin, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Brochothrix thermosphacter",
"methylation_site": ""
},
"tsp32ii": {
"target_site": "T^CGA",
"name": "Tsp32II",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Williams, R.A.D., (1995) Biochem. J., vol. 312, pp. 505-510."
],
"prototype": "TaqI",
"organism": "Thermus strain 32",
"methylation_site": ""
},
"hpych4iv": {
"target_site": "A^CGT",
"name": "HpyCH4IV",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "M.J. Blaser",
"references": [
"Morgan, R.D., Xu, Q., US Patent Office, 2001."
],
"prototype": "MaeII",
"organism": "Helicobacter pylori CH4",
"methylation_site": "2(5)"
},
"eco153i": {
"target_site": "CCNGG",
"name": "Eco153I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Escherichia coli RFL153",
"methylation_site": ""
},
"bmsi": {
"target_site": "GCATC(5/9)",
"name": "BmsI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G358",
"references": [
"Vitkute, J., Lauciuniene, N., Lapcinskaja, S., Trinkunaite, L., Zakareviciene, L., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Bacillus massiliensis RFL101",
"methylation_site": ""
},
"nmei": {
"target_site": "?",
"name": "NmeI",
"suppliers": [],
"source": "R. Sparling",
"references": [
"Sparling, R., Bhatti, A.R., (1984) Microbios, vol. 41, pp. 73-79."
],
"prototype": "",
"organism": "Neisseria meningitidis DRES-W34",
"methylation_site": ""
},
"asp130i": {
"target_site": "ATCGAT",
"name": "Asp130I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "ClaI",
"organism": "Anabaena species 130",
"methylation_site": ""
},
"bcodi": {
"target_site": "GTCTC(1/5)",
"name": "BcoDI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "DSM 17136",
"references": [
"Chan, S.H., Xu, S.Y., Unpublished observations.",
"Fulton, L., Clifton, S., Fulton, B., Xu, J., Minx, P., Mardis, E.R., Wilson, R.K., Unpublished observations.",
"Fulton, L., Clifton, S., Fulton, B., Xu, J., Minx, P., Pepin, K.H., Johnson, M., Thiruvilangam, P., Bhonagiri, V., Nash, W.E., Mardis, E.R., Wilson, R.K., Unpublished observations."
],
"prototype": "BsmAI",
"organism": "Bacteroides coprocola DSM 17136",
"methylation_site": ""
},
"ssai": {
"target_site": "?",
"name": "SsaI",
"suppliers": [],
"source": "ATCC 13518",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Staphylococcus saprophyticus",
"methylation_site": ""
},
"ahdi": {
"target_site": "GACNNN^NNGTC",
"name": "AhdI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 724",
"references": [
"Chang, Z., Morgan, R., Unpublished observations.",
"Lunnen, K.L., Tao, C., Wilson, G.G., Unpublished observations.",
"Polisson, C., Unpublished observations."
],
"prototype": "Eam1105I",
"organism": "Aeromonas hydrophila",
"methylation_site": ""
},
"uba1322i": {
"target_site": "GGCC",
"name": "Uba1322I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1322",
"methylation_site": ""
},
"m.csp68kv": {
"target_site": "GGCC",
"name": "M.Csp68KV",
"suppliers": [],
"source": "ATCC 51142",
"references": [
"Soper, B.W., Hollister, W.R., Reddy, K.J., (1998) J. Mar. Biotechnol., vol. 6, pp. 183-185.",
"Welsh, E.A., Liberton, M., Stockel, J., Sato, H., Loh, T., Wang, C., Wollam, A., Fulton, R.S., Clifton, S.W., Jacobs, J.M., Aurora, R., Sherman, L.A., Smith, R.D., Wilson, R.K., Pakrasi, H.B., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Cyanothece species BH68K",
"methylation_site": "?(4)"
},
"bsp121i": {
"target_site": "GCATGC",
"name": "Bsp121I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "SphI",
"organism": "Bacillus species RFL121",
"methylation_site": ""
},
"aitii": {
"target_site": "RGATCY",
"name": "AitII",
"suppliers": [],
"source": "NEB 443",
"references": [
"Polisson, C., McMahon, M., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Aquaspirillum itersonii",
"methylation_site": ""
},
"pspomi": {
"target_site": "G^GGCCC",
"name": "PspOMI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "NEB 1783",
"references": [
"Belichenko, O.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "ApaI",
"organism": "Pseudomonas species OM2164",
"methylation_site": ""
},
"rma510i": {
"target_site": "CTAG",
"name": "Rma510I",
"suppliers": [],
"source": "ITI 510",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"bsrxi": {
"target_site": "TCTAGA",
"name": "BsrXI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "XbaI",
"organism": "Bacillus stearothermophilus X173",
"methylation_site": ""
},
"m.ngobix": {
"target_site": "GTANNNNNCTC",
"name": "M.NgoBIX",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Piekarowicz, A., Yuan, R., Stein, D.C., (1988) Nucleic Acids Res., vol. 16, pp. 5957-5972."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae WR302",
"methylation_site": "9(5)"
},
"bshvi": {
"target_site": "AT^CGAT",
"name": "BshVI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 182",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus sphaericus CKJ18",
"methylation_site": ""
},
"spei": {
"target_site": "A^CTAGT",
"name": "SpeI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 13923",
"references": [
"Chang, Z., Morgan, R., Unpublished observations.",
"Comb, D.G., Grandoni, R., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Sphaerotilus natans",
"methylation_site": ""
},
"eco170i": {
"target_site": "CCWGG",
"name": "Eco170I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL170",
"methylation_site": ""
},
"sspm2ii": {
"target_site": "GRCGYC",
"name": "SspM2II",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "AcyI",
"organism": "Spirulina species M2",
"methylation_site": ""
},
"bst4ci": {
"target_site": "ACN^GT",
"name": "Bst4CI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shinkarenko, N.M., Schevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Bacillus stearothermophilus 4C",
"methylation_site": ""
},
"m.ngogii": {
"target_site": "GGCC",
"name": "M.NgoGII",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Piekarowicz, A., Yuan, R., Stein, D.C., (1988) Nucleic Acids Res., vol. 16, pp. 5957-5972."
],
"prototype": "HaeIII",
"organism": "Neisseria gonorrhoeae WR220",
"methylation_site": "3(5)"
},
"eco47ii": {
"target_site": "GGNCC",
"name": "Eco47II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Butkus, V., (1983) FEBS Lett., vol. 161, pp. 213-216.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Stankevicius, K., Povilionis, P., Lubys, A., Menkevicius, S., Janulaitis, A., (1995) Gene, vol. 157, pp. 49-53."
],
"prototype": "AsuI",
"organism": "Escherichia coli RFL47",
"methylation_site": "?(5)"
},
"abri": {
"target_site": "C^TCGAG",
"name": "AbrI",
"suppliers": [],
"source": "ATCC 29711",
"references": [
"Schwabe, G., Helke, A., Klinkmueller, W., Unpublished observations.",
"Schwabe, G., Posseckert, G., Klingmuller, W., (1985) Gene, vol. 39, pp. 113-116."
],
"prototype": "XhoI",
"organism": "Azospirillum brasilense",
"methylation_site": ""
},
"eco98i": {
"target_site": "AAGCTT",
"name": "Eco98I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Escherichia coli RFL98",
"methylation_site": ""
},
"esphk24i": {
"target_site": "YGGCCR",
"name": "EspHK24I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Enterobacter species 24I",
"methylation_site": ""
},
"rcei": {
"target_site": "CATCGAC(20/18)",
"name": "RceI",
"suppliers": [],
"source": "ATCC 51521",
"references": [
"Morgan, R.D., Dwinell, E.A., Unpublished observations.",
"Touchman, J.W., Bauer, C., Blankenship, R.E., Unpublished observations."
],
"prototype": "",
"organism": "Rhodospirillum centenum SW",
"methylation_site": "?(6)"
},
"asisi": {
"target_site": "GCGAT^CGC",
"name": "AsiSI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations.",
"Zhu, Z., Xu, S.-Y., Unpublished observations.",
"Zhu, Z., Xu, S.-Y., US Patent Office, 2003."
],
"prototype": "SgfI",
"organism": "Arthrobacter species S",
"methylation_site": "?(5)"
},
"avrii": {
"target_site": "C^CTAGG",
"name": "AvrII",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "E.C. Rosenvold",
"references": [
"Lunnen, K.D., Moran, L.S., Slatko, B.E., Wilson, G.G., Unpublished observations.",
"Lunnen, K.D., Moran, L.S., Slatko, B.E., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Rosenvold, E.C., Szybalski, W., Unpublished observations."
],
"prototype": "",
"organism": "Anabaena variabilis uw",
"methylation_site": ""
},
"bsp82i": {
"target_site": "TTCGAA",
"name": "Bsp82I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species RFL82",
"methylation_site": ""
},
"bsibi": {
"target_site": "GATNN^NNATC",
"name": "BsiBI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Mok, Y.K., Clark, D.R., Kam, K.M., Shaw, P.C., (1990) Nucleic Acids Res., vol. 18, pp. 6740."
],
"prototype": "BsaBI",
"organism": "Bacillus species",
"methylation_site": ""
},
"hin1076iii": {
"target_site": "AAGCTT",
"name": "Hin1076III",
"suppliers": [],
"source": "J. Stuy",
"references": [
"Olson, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Haemophilus influenzae serotype b, 1076",
"methylation_site": ""
},
"pstii": {
"target_site": "CTGATG(25/27)",
"name": "PstII",
"suppliers": [],
"source": "ATCC 49762",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Sears, A., Peakman, L.J., Wilson, G.G., Szczelkun, M.D., (2005) Nucleic Acids Res., vol. 33, pp. 4775-4787."
],
"prototype": "",
"organism": "Providencia stuartii 164",
"methylation_site": "?(6)"
},
"cjui": {
"target_site": "CAYNNNNNRTG",
"name": "CjuI",
"suppliers": [],
"source": "Fermentas G311",
"references": [
"Vitkute, J., Lauciuniene, N., Riauba, L., Capskaja, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Campylobacter jejuni RFL1",
"methylation_site": ""
},
"bst1i": {
"target_site": "CC^WGG",
"name": "Bst1I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus 1",
"methylation_site": ""
},
"bpoai": {
"target_site": "AT^TAAT",
"name": "BpoAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Eldarov, M.A., Samko, O.T., Karpichev, I.V., Kalugin, A.A., Anikeitcheva, N.V., Scryabin, K.G., Unpublished observations.",
"Sokolov, N.N., Kalugin, A.A., Samko, O.T., Omeljanuk, N.M., Unpublished observations."
],
"prototype": "VspI",
"organism": "Bacillus polymyxa",
"methylation_site": ""
},
"bli161i": {
"target_site": "GGTCTC",
"name": "Bli161I",
"suppliers": [],
"source": "C. Parini",
"references": [
"Parini, C., Fortina, M.G., (1995) FEMS Microbiol. Lett., vol. 132, pp. 285-289."
],
"prototype": "Eco31I",
"organism": "Bacillus licheniformis 16.1",
"methylation_site": ""
},
"aspni": {
"target_site": "GGN^NCC",
"name": "AspNI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Frey, B., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Anabaena species J3",
"methylation_site": ""
},
"sfii": {
"target_site": "GGCCNNNN^NGGCC",
"name": "SfiI",
"suppliers": [
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 15051",
"references": [
"Qiang, B.-Q., Schildkraut, I., (1984) Nucleic Acids Res., vol. 12, pp. 4507-4515.",
"Sasaki, A., Oka, M., Shigenori, M., Japanese Patent Office, 1992.",
"Van Cott, E.M., Moran, L.S., Slatko, B.E., Wilson, G.G., Unpublished observations.",
"Vancott, E.M., European Patent Office, 1995.",
"Wentzell, L.M., Oram, M., Halford, S.E., (1994) Biochem. Soc. Trans., vol. 22, pp. 302S."
],
"prototype": "",
"organism": "Streptomyces fimbriatus",
"methylation_site": "?(4)"
},
"asigi": {
"target_site": "A^CCGGT",
"name": "AsiGI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AgeI",
"organism": "Arthrobacter species G",
"methylation_site": ""
},
"eco143i": {
"target_site": "GCGCGC",
"name": "Eco143I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Escherichia coli RFL143",
"methylation_site": ""
},
"bst2464i": {
"target_site": "GGATCC",
"name": "Bst2464I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Bacillus stearothermophilus 2464",
"methylation_site": ""
},
"bstnsi": {
"target_site": "RCATG^Y",
"name": "BstNSI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NspI",
"organism": "Bacillus stearothermophilus 1161NS",
"methylation_site": ""
},
"ecop15i": {
"target_site": "CAGCAG(25/27)",
"name": "EcoP15I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "W. Arber",
"references": [
"Ahmad, I., Rao, D.N., (1994) Gene, vol. 142, pp. 67-71.",
"Hadi, S.M., Bachi, B., Shepherd, J.C.W., Yuan, R., Ineichen, K., Bickle, T.A., (1979) J. Mol. Biol., vol. 134, pp. 655-666.",
"Meisel, A., Kruger, D.H., Bickle, T.A., (1991) Nucleic Acids Res., vol. 19, pp. 3997.",
"Moencke-Buchner, E., Mackeldanz, P., Krueger, D.H., Reuter, M., (2004) J. Biotechnol., vol. 114, pp. 99-106.",
"Reiser, J., Yuan, R., (1977) J. Biol. Chem., vol. 252, pp. 451-456.",
"Wagenfuhr, K., Pieper, S., Mackeldanz, P., Linscheid, M., Kruger, D.H., Reuter, M., (2007) J. Mol. Biol., vol. 366, pp. 93-102."
],
"prototype": "",
"organism": "Escherichia coli P15",
"methylation_site": "5(6)"
},
"mlu40i": {
"target_site": "GDGCHC",
"name": "Mlu40I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "SduI",
"organism": "Micrococcus luteus 40",
"methylation_site": ""
},
"faei": {
"target_site": "CATG^",
"name": "FaeI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NlaIII",
"organism": "Flavobacterium aquatile N3",
"methylation_site": ""
},
"mluni": {
"target_site": "TGG^CCA",
"name": "MluNI",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "BalI",
"organism": "Micrococcus luteus N",
"methylation_site": ""
},
"bseb631i": {
"target_site": "GCCNNNNNGGC",
"name": "BseB631I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BglI",
"organism": "Bacillus species 6-3-1",
"methylation_site": ""
},
"faubi": {
"target_site": "?",
"name": "FauBI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Rahman, H., Unpublished observations."
],
"prototype": "",
"organism": "Flavobacterium aureus B",
"methylation_site": ""
},
"bstz4i": {
"target_site": "CYCGRG",
"name": "BstZ4I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Bacillus stearothermophilus N136",
"methylation_site": ""
},
"nsici": {
"target_site": "GAT^ATC",
"name": "NsiCI",
"suppliers": [],
"source": "C.A. Hart",
"references": [
"Sullivan, K.M., Macdonald, H.J., Saunders, J.R., (1987) FEMS Microbiol. Lett., vol. 44, pp. 389-393."
],
"prototype": "EcoRV",
"organism": "Neisseria sicca C351",
"methylation_site": ""
},
"rhpii": {
"target_site": "?",
"name": "RhpII",
"suppliers": [],
"source": "ATCC 19148",
"references": [
"Hurlin, P., Schildkraut, I., Unpublished observations."
],
"prototype": "",
"organism": "Rhodococcus species",
"methylation_site": ""
},
"uba1353i": {
"target_site": "ATGCAT",
"name": "Uba1353I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Unidentified bacterium RFL1353",
"methylation_site": ""
},
"eco1348i": {
"target_site": "GGANNNNNNTATC",
"name": "Eco1348I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco777I",
"organism": "Escherichia coli EC1348",
"methylation_site": ""
},
"uba1452i": {
"target_site": "TTCGAA",
"name": "Uba1452I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Unidentified bacterium RFL1452",
"methylation_site": ""
},
"eco57i": {
"target_site": "CTGAAG(16/14)",
"name": "Eco57I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Maneliene, Z., Klimasauskas, S., Butkus, V., (1992) Nucleic Acids Res., vol. 20, pp. 6043-6049.",
"Petrusyte, M.P., Bitinaite, J.B., Kersulyte, D.R., Menkevicius, S.J., Butkus, V.V., Janulaitis, A., (1987) Dokl. Akad. Nauk., vol. 295, pp. 1250-1253."
],
"prototype": "",
"organism": "Escherichia coli RFL57",
"methylation_site": "5(6)"
},
"bpub5i": {
"target_site": "C^GTACG",
"name": "BpuB5I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Zhao, Z.-H., Yung, M.-H., Cui, T., Wang, Y.-Z., Shaw, P.C., (1992) Nucleic Acids Res., vol. 20, pp. 1156."
],
"prototype": "SplI",
"organism": "Bacillus pumilus",
"methylation_site": ""
},
"mpui": {
"target_site": "CTCGAG",
"name": "MpuI",
"suppliers": [],
"source": "ATCC 15835",
"references": [
"Meyertons, J.L., Tilley, B.C., Lechevalier, M.P., Lechevalier, H.A., (1987) J. Ind. Microbiol., vol. 2, pp. 293-303."
],
"prototype": "XhoI",
"organism": "Micromonospora purpurea",
"methylation_site": ""
},
"uba1157i": {
"target_site": "GGGCCC",
"name": "Uba1157I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ApaI",
"organism": "Unidentified bacterium RFL1157",
"methylation_site": ""
},
"bmahi": {
"target_site": "GAATGC(1/-1)",
"name": "BmaHI",
"suppliers": [],
"source": "Vivantis 12",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"bsthpi": {
"target_site": "GTT^AAC",
"name": "BstHPI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HpaI",
"organism": "Bacillus stearothermophilus HP",
"methylation_site": ""
},
"m.zmadrm1": {
"target_site": "?",
"name": "M.ZmaDRM1",
"suppliers": [],
"source": "R.L. Phillips",
"references": [
"Cao, X., Springer, N.M., Muszynski, M.G., Phillips, R.L., Kaeppler, S., Jacobsen, S.E., (2000) Proc. Natl. Acad. Sci. U. S. A., vol. 97, pp. 4979-4984."
],
"prototype": "",
"organism": "Zea mays",
"methylation_site": "?(5)"
},
"cpa1150i": {
"target_site": "CGCG",
"name": "Cpa1150I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Clostridium pasteurianum 1150",
"methylation_site": ""
},
"paepi": {
"target_site": "CTGCA^G",
"name": "PaePI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravetz, A.N., Tarutina, Z.E., Solonin, A.S., (1991) Nucleic Acids Res., vol. 19, pp. 4781."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa 4148",
"methylation_site": ""
},
"afa16ri": {
"target_site": "CGAT^CG",
"name": "Afa16RI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kita, K., Hiraoka, N., Kishimoto, N., Sugio, T., Tano, T., (1990) J. Ferment. Bioeng., vol. 69, pp. 60-62."
],
"prototype": "PvuI",
"organism": "Acidiphilium species 16R",
"methylation_site": ""
},
"bmei": {
"target_site": "?",
"name": "BmeI",
"suppliers": [],
"source": "J. Upcroft",
"references": [
"Gelinas, R.E., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"btsi": {
"target_site": "GCAGTG(2/0)",
"name": "BtsI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1137",
"references": [
"Ganatra, M., Krotee, S., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Pan, X.S., Morgan, R., Unpublished observations.",
"Xu, S.Y., Zhu, Z., Zhang, P., Chan, S.H., Samuelson, J.C., Xiao, J., Ingalls, D., Wilson, G.G., (2007) Nucleic Acids Res., vol. 35, pp. 4608-4618."
],
"prototype": "",
"organism": "Bacillus thermoglucosidasius",
"methylation_site": ""
},
"bstdsi": {
"target_site": "C^CRYGG",
"name": "BstDSI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "DsaI",
"organism": "Bacillus stearothermophilus DS",
"methylation_site": ""
},
"bsmni": {
"target_site": "GCATC",
"name": "BsmNI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Bacillus stearothermophilus N365",
"methylation_site": ""
},
"asii": {
"target_site": "G^GATCC",
"name": "AsiI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Korolev, S.V., Sokolov, N.N., Rina, M., Eldarov, M.A., Omelyanyuk, N.M., Markaki, M., Skryabin, K.G., Bouriotis, V., (1998) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 32-35."
],
"prototype": "BamHI",
"organism": "Azotobacter species",
"methylation_site": ""
},
"hpyf25i": {
"target_site": "CTNAG",
"name": "HpyF25I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL25",
"methylation_site": ""
},
"bstvi": {
"target_site": "C^TCGAG",
"name": "BstVI",
"suppliers": [],
"source": "C. Vasquez",
"references": [
"Barra, R., Chiong, M., Gonzalez, E., Vasquez, C., (1988) Biochem. J., vol. 255, pp. 699-703.",
"Gonzalez, E., Padilla, C., Saavedra, C., Vasquez, C., (1994) Microbiology, vol. 140, pp. 1337-1340.",
"Vasquez, C., (1985) Biochem. Int., vol. 10, pp. 655-662.",
"Vasquez, C., (1988) Arch. Biol. Med. Exp. (Santiago), vol. 21."
],
"prototype": "XhoI",
"organism": "Bacillus stearothermophilus V",
"methylation_site": "5(6)"
},
"afa22mi": {
"target_site": "CGAT^CG",
"name": "Afa22MI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kita, K., Hiraoka, N., Kishimoto, N., Sugio, T., Tano, T., (1990) J. Ferment. Bioeng., vol. 69, pp. 60-62.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Yamaoka, S., Tamura, T., Takenobu, H., Kojo, T., Tanaka, H., Inagaki, K., (2003) Sci. Reports. Fac. Agric. Okayama Univ., vol. 92, pp. 9-15."
],
"prototype": "PvuI",
"organism": "Acidocella facilis",
"methylation_site": "?(5)"
},
"blui": {
"target_site": "C^TCGAG",
"name": "BluI",
"suppliers": [],
"source": "ATCC 15830",
"references": [
"Gingeras, T.R., Myers, P.A., Olson, J.A., Hanberg, F.A., Roberts, R.J., (1978) J. Mol. Biol., vol. 118, pp. 113-122."
],
"prototype": "XhoI",
"organism": "Brevibacterium luteum",
"methylation_site": ""
},
"bce22i": {
"target_site": "G^GNCC",
"name": "Bce22I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Bacillus cereus 22",
"methylation_site": ""
},
"bsanii": {
"target_site": "CTGCAG",
"name": "BsaNII",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus stearothermophilus CPW1",
"methylation_site": ""
},
"bceai": {
"target_site": "ACGGC(12/14)",
"name": "BceAI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1288",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nkenfou, C., Unpublished observations.",
"Nkenfou, C., (2002) Ph.D. Thesis, University of Yaounde I, Cameroon (Yaounde), pp. 1-226.",
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "BcefI",
"organism": "Bacillus cereus 1315",
"methylation_site": "?(4)"
},
"bsp1894i": {
"target_site": "G^GNCC",
"name": "Bsp1894I",
"suppliers": [],
"source": "KCTC 1188",
"references": [
"Ryu, C.J., Lee, C.H., Yoo, O.J., (1989) Korean Biochem. J., vol. 22, pp. 444-447."
],
"prototype": "AsuI",
"organism": "Bacillus sphaericus 1894",
"methylation_site": ""
},
"bth9415i": {
"target_site": "ATCGAT",
"name": "Bth9415I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "ClaI",
"organism": "Bacillus thuringiensis ssp. monterrey",
"methylation_site": ""
},
"spapiii": {
"target_site": "CAGCTG",
"name": "SpaPIII",
"suppliers": [],
"source": "D. Borthakur",
"references": [
"Tragut, V., Xiao, J., Bylina, E.J., Borthakur, D., (1995) J. Appl. Phycol., vol. 7, pp. 561-564."
],
"prototype": "PvuII",
"organism": "Spirulina platensis strain pacifica",
"methylation_site": ""
},
"hpyf49i": {
"target_site": "TCGA",
"name": "HpyF49I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL49",
"methylation_site": ""
},
"m.nmaphich1i": {
"target_site": "GATC",
"name": "M.NmaPhiCh1I",
"suppliers": [],
"source": "A. Witte",
"references": [
"Baranyi, U., Klein, R., Lubitz, W., Kruger, D.H., Witte, A., (2000) Mol. Microbiol., vol. 35, pp. 1168-1179."
],
"prototype": "MboI",
"organism": "Natrialba magadii phage PhiCh1",
"methylation_site": "2(6)"
},
"hpyf72iii": {
"target_site": "GANTC",
"name": "HpyF72III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL72",
"methylation_site": ""
},
"bse64i": {
"target_site": "G^GTNACC",
"name": "Bse64I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BstEII",
"organism": "Bacillus species 64",
"methylation_site": ""
},
"bnii": {
"target_site": "GCNNGC",
"name": "BniI",
"suppliers": [],
"source": "Fermentas G361",
"references": [
"Vitkute, J., Spiliauskaite, K., Trinkunaite, L., Vitkute, J., Janulaitis, A., Unpublished observations."
],
"prototype": "Cac8I",
"organism": "Bacillus niacini RFL102",
"methylation_site": ""
},
"bbvai": {
"target_site": "GAANN^NNTTC",
"name": "BbvAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Butkus, V., Vaitkevicius, D., Anikeitcheva, N.V., Fitsner, A.B., Kalugin, A.A., Janulaitis, A., Unpublished observations.",
"Sokolov, N.N., Samko, O.T., Anikeitcheva, N.V., Kalugin, A.A., Khoroshutina, E.B., Plutalov, O.V., Birikh, K.R., Berlin, Y.A., (1994) Bioorg. Khim., vol. 20, pp. 1334-1341."
],
"prototype": "XmnI",
"organism": "Bacillus brevis A",
"methylation_site": ""
},
"eco40i": {
"target_site": "CCWGG",
"name": "Eco40I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Adomaviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL40",
"methylation_site": ""
},
"bstx2i": {
"target_site": "R^GATCY",
"name": "BstX2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Bacillus stearothermophilus X2",
"methylation_site": ""
},
"bst4qi": {
"target_site": "GGWCC",
"name": "Bst4QI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus stearothermophilus 4Q",
"methylation_site": ""
},
"ubaf14i": {
"target_site": "CCANNNNNTCG",
"name": "UbaF14I",
"suppliers": [],
"source": "Fermentas G336",
"references": [
"Vitkute, J., Lauciuniene, N., Capskaja, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Unidentified bacterium F14",
"methylation_site": ""
},
"pluti": {
"target_site": "GGCGC^C",
"name": "PluTI",
"suppliers": [],
"source": "E. Duchaud",
"references": [
"Duchaud, E. et al., (2003) Nat. Biotechnol., vol. 21, pp. 1307-1313.",
"Khan, F., Furuta, Y., Kawai, M., Kaminska, K.H., Ishikawa, K., Bujnicki, J.M., Kobayashi, I., (2010) Nucleic Acids Res., vol. 38, pp. 3019-3030."
],
"prototype": "NarI",
"organism": "Photorhabdus luminescens",
"methylation_site": "?(5)"
},
"hpyf38i": {
"target_site": "GANTC",
"name": "HpyF38I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL38",
"methylation_site": ""
},
"eco1323i": {
"target_site": "GGANNNNNNNNATGC",
"name": "Eco1323I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco377I",
"organism": "Escherichia coli EC1323",
"methylation_site": ""
},
"atei": {
"target_site": "CCATGG",
"name": "AteI",
"suppliers": [],
"source": "Glaxo 2523C",
"references": [
"Earle-Hughes, J., Roberts, R.J., Unpublished observations."
],
"prototype": "NcoI",
"organism": "Actinoplanes teochomyceticus",
"methylation_site": ""
},
"saqai": {
"target_site": "T^TAA",
"name": "SaqAI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G362",
"references": [
"Lauciuniene, N., Valiulyte, D., Capskaja, L., Trinkunaite, L., Vitkute, J., Janulaitis, A., Unpublished observations.",
"Zakareviciene, L., Lubys, A., Unpublished observations."
],
"prototype": "MseI",
"organism": "Salinibacterium aquaticus RFL1",
"methylation_site": ""
},
"eco128i": {
"target_site": "CCWGG",
"name": "Eco128I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations.",
"Waleron, K., Waleron, M., Osipiuk, J., Podhajska, A.J., Lojkowska, E., (2006) J. Appl. Microbiol., vol. 100, pp. 343-351."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL128",
"methylation_site": "?(5)"
},
"cspi": {
"target_site": "CG^GWCCG",
"name": "CspI",
"suppliers": [
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)"
],
"source": "M. Nelson",
"references": [
"Nelson, M., McClelland, M., Unpublished observations."
],
"prototype": "RsrII",
"organism": "Corynebacterium species",
"methylation_site": ""
},
"drdbi": {
"target_site": "CCGCGG",
"name": "DrdBI",
"suppliers": [],
"source": "R.G.E. Murray",
"references": [
"Polisson, C., Murray, R.G.E., Unpublished observations."
],
"prototype": "SacII",
"organism": "Deinococcus radiodurans 288",
"methylation_site": ""
},
"ama87i": {
"target_site": "C^YCGRG",
"name": "Ama87I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Alteromonas macleodii 87",
"methylation_site": ""
},
"bsrvi": {
"target_site": "GCAGC",
"name": "BsrVI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "BbvI",
"organism": "Bacillus stearothermophilus V35",
"methylation_site": ""
},
"sau17i": {
"target_site": "GGNCC",
"name": "Sau17I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Staphylococcus aureus RFL17",
"methylation_site": ""
},
"uba1430i": {
"target_site": "ATCGAT",
"name": "Uba1430I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1430",
"methylation_site": ""
},
"vcho66i": {
"target_site": "GGNCC",
"name": "VchO66I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "AsuI",
"organism": "Vibrio cholerae 993-75",
"methylation_site": ""
},
"uba1175i": {
"target_site": "GGCC",
"name": "Uba1175I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1175",
"methylation_site": ""
},
"bspti": {
"target_site": "C^TTAAG",
"name": "BspTI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AflII",
"organism": "Bacillus species RFL1265",
"methylation_site": ""
},
"paci": {
"target_site": "TTAAT^TAA",
"name": "PacI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "NEB 585",
"references": [
"Kur, J., (1993) Acta Microbiol. Pol., vol. 42, pp. 145-150.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., European Patent Office, 1994.",
"Polisson, C., Fu, P., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas alcaligenes",
"methylation_site": ""
},
"bce4i": {
"target_site": "GCNNNNN^NNGC",
"name": "Bce4I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Kashirina, J.G., Tomilova, J.E., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MwoI",
"organism": "Bacillus cereus B4",
"methylation_site": ""
},
"cbii": {
"target_site": "TT^CGAA",
"name": "CbiI",
"suppliers": [],
"source": "T. Mitsuoka",
"references": [
"Murakami, M., Ozawa, O., Kanematsu, T., Yamada, Y., (1991) Nucleic Acids Res., vol. 19, pp. 3458."
],
"prototype": "AsuII",
"organism": "Clostridium bifermentans B-4",
"methylation_site": ""
},
"smuei": {
"target_site": "G^GWCC",
"name": "SmuEI",
"suppliers": [],
"source": "I. Nasz",
"references": [
"Molnar, A., Geck, P., Orosz, A., Kulcsar, P., Nasz, I., (1991) Acta Microbiol. Hung., vol. 38, pp. 55-60."
],
"prototype": "AvaII",
"organism": "Streptococcus mutans E",
"methylation_site": ""
},
"asp86ii": {
"target_site": "?",
"name": "Asp86II",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Anabaena species 86",
"methylation_site": ""
},
"ngoav-1": {
"target_site": "?",
"name": "NgoAV-1",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Lewis, L.A. et al., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(6)"
},
"asp21hi": {
"target_site": "RGATCY",
"name": "Asp21HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Acidiphilium species 21H",
"methylation_site": ""
},
"hpyf67i": {
"target_site": "CTNAG",
"name": "HpyF67I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL67",
"methylation_site": ""
},
"uba1159i": {
"target_site": "GRGCYC",
"name": "Uba1159I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1159",
"methylation_site": ""
},
"bciai": {
"target_site": "?",
"name": "BciAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Eldarov, M.A., Anikeitcheva, N.V., Karpichev, I.V., Samko, O.T., Fitsner, A.B., Kalugin, A.A., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus circulans A",
"methylation_site": ""
},
"sph1719i": {
"target_site": "CTCGAG",
"name": "Sph1719I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces phaeopurpureus 1719",
"methylation_site": ""
},
"rsai": {
"target_site": "GT^AC",
"name": "RsaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "S. Kaplan",
"references": [
"Lunnen, K.D., Meixsell, T., Morgan, R.D., Wilson, G.G., European Patent Office, 2001.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F., (1980) J. Bacteriol., vol. 142, pp. 380-383.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Rhodopseudomonas sphaeroides",
"methylation_site": "4(4)"
},
"swai": {
"target_site": "ATTT^AAAT",
"name": "SwaI",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)"
],
"source": "B. Frey",
"references": [
"Higgins, L.S., Kong, H., Unpublished observations.",
"Lechner, M., Frey, B., Laue, F., Ankenbauer, W., Schmitz, G., (1992) Fresenius Z. Anal. Chem., vol. 343, pp. 121-122."
],
"prototype": "",
"organism": "Staphylococcus warneri",
"methylation_site": "?(6)"
},
"bsu121i": {
"target_site": "?",
"name": "Bsu121I",
"suppliers": [],
"source": "A.R. Reddy",
"references": [
"Jutur, P.P., Reddy, A.R., (2002) Mol. Biol. Rep., vol. 29, pp. 383-385."
],
"prototype": "",
"organism": "Bacillus subtilis strain 121",
"methylation_site": ""
},
"bth1795i": {
"target_site": "CTGGAG",
"name": "Bth1795I",
"suppliers": [],
"source": "NEB 1326",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "GsuI",
"organism": "Bacillus thuringiensis 1795",
"methylation_site": ""
},
"fsui": {
"target_site": "GACNNNGTC",
"name": "FsuI",
"suppliers": [],
"source": "ATCC 13718",
"references": [
"Stote, R., Morgan, R., Unpublished observations."
],
"prototype": "Tth111I",
"organism": "Flavobacterium suaveolens",
"methylation_site": ""
},
"asp713i": {
"target_site": "CTGCA^G",
"name": "Asp713I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Wersig, U., Bolton, B., Frey, B., Jarsch, M., Kessler, C., Unpublished observations."
],
"prototype": "PstI",
"organism": "Achromobacter species 713",
"methylation_site": ""
},
"sspjii": {
"target_site": "GRCGYC",
"name": "SspJII",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "AcyI",
"organism": "Spirulina species J",
"methylation_site": ""
},
"hpych4v": {
"target_site": "TG^CA",
"name": "HpyCH4V",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "M.J. Blaser",
"references": [
"Morgan, R.D., Xu, Q., US Patent Office, 2000."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori CH4",
"methylation_site": ""
},
"bstmwi": {
"target_site": "GCNNNNN^NNGC",
"name": "BstMWI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Nadeev, A.N., Dedkov, V.S., Tomilova, J.T., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MwoI",
"organism": "Bacillus stearothermophilus MW",
"methylation_site": ""
},
"uba1267i": {
"target_site": "CCGG",
"name": "Uba1267I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Unidentified bacterium RFL1267",
"methylation_site": ""
},
"fbai": {
"target_site": "T^GATCA",
"name": "FbaI",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "ATCC 33487",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations.",
"Yamamoto, K., Hiraoka, N., Unpublished observations."
],
"prototype": "BclI",
"organism": "Flavobacterium balustinum",
"methylation_site": ""
},
"accb1i": {
"target_site": "G^GYRCC",
"name": "AccB1I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Acinetobacter calcoaceticus B1",
"methylation_site": ""
},
"bsp22i": {
"target_site": "CTGGAG",
"name": "Bsp22I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "GsuI",
"organism": "Bacillus species RFL22",
"methylation_site": ""
},
"bmeu1594i": {
"target_site": "GGCC",
"name": "BmeU1594I",
"suppliers": [],
"source": "NEB 1594",
"references": [
"Abila, P., Nanyonjo, H., Egwang, T.G., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"uba1225i": {
"target_site": "CTGCAG",
"name": "Uba1225I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1225",
"methylation_site": ""
},
"eco35i": {
"target_site": "GRGCYC",
"name": "Eco35I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL35",
"methylation_site": ""
},
"mniii": {
"target_site": "CCGG",
"name": "MniII",
"suppliers": [],
"source": "ATCC 19966",
"references": [
"Jiang, B.D., Myers, P., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Moraxella nonliquefaciens",
"methylation_site": ""
},
"maei": {
"target_site": "C^TAG",
"name": "MaeI",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "K.O. Stetter",
"references": [
"Schmid, K., Thomm, M., Laminet, A., Laue, F.G., Kessler, C., Stetter, K.O., Schmitt, R., (1984) Nucleic Acids Res., vol. 12, pp. 2619-2628."
],
"prototype": "",
"organism": "Methanococcus aeolicus PL-15/H",
"methylation_site": ""
},
"bstswi": {
"target_site": "ATTT^AAAT",
"name": "BstSWI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Bondar, T.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SwaI",
"organism": "Bacillus stearothermophilus SW",
"methylation_site": ""
},
"bbrpi": {
"target_site": "CAC^GTG",
"name": "BbrPI",
"suppliers": [
"Roche Applied Science (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "U. Mayr",
"references": [
"Vesely, Z., Schmitz, G.G., Jarsch, M., Kessler, C., (1990) Nucleic Acids Res., vol. 18, pp. 3423."
],
"prototype": "PmaCI",
"organism": "Bacillus brevis",
"methylation_site": ""
},
"cali": {
"target_site": "?",
"name": "CalI",
"suppliers": [],
"source": "W.C. Trentini",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Caryophanon latum H7",
"methylation_site": ""
},
"mae843ii": {
"target_site": "?",
"name": "Mae843II",
"suppliers": [],
"source": "NIES 843",
"references": [
"Le, T.K.T., Morgan, R., Unpublished observations."
],
"prototype": "",
"organism": "Microcystis aeruginosa",
"methylation_site": "?(6)"
},
"kzo49i": {
"target_site": "G^GWCC",
"name": "Kzo49I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Kurthia zopfii 49",
"methylation_site": ""
},
"bssxi": {
"target_site": "GCNGC",
"name": "BssXI",
"suppliers": [],
"source": "G. Elgar",
"references": [
"Elgar, G., Unpublished observations."
],
"prototype": "Fnu4HI",
"organism": "Bacillus species",
"methylation_site": ""
},
"bsu8646i": {
"target_site": "GGATCC",
"name": "Bsu8646I",
"suppliers": [],
"source": "NCIB 88646",
"references": [
"Leung, S.M., Chan, K.Y., Suen, Y.K., Shaw, P.C., (1989) Nucleic Acids Res., vol. 17, pp. 10133."
],
"prototype": "BamHI",
"organism": "Bacillus subtilis",
"methylation_site": ""
},
"uba1197i": {
"target_site": "ATCGAT",
"name": "Uba1197I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1197",
"methylation_site": ""
},
"mlei": {
"target_site": "GGATCC",
"name": "MleI",
"suppliers": [],
"source": "ATCC 540",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Micrococcus luteus",
"methylation_site": ""
},
"apctr183i": {
"target_site": "TGCGCA",
"name": "ApcTR183I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "MstI",
"organism": "Aphanizomenon species TR183",
"methylation_site": ""
},
"kzo9i": {
"target_site": "^GATC",
"name": "Kzo9I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Rechkunova, N.I., Grinev, A.A., Dedkov, V.S., (1989) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 15, pp. 25-26."
],
"prototype": "MboI",
"organism": "Kurthia zopfii 9",
"methylation_site": ""
},
"csp4i": {
"target_site": "ATCGAT",
"name": "Csp4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Corynebacterium species RFL4",
"methylation_site": ""
},
"bscmi": {
"target_site": "GRGCYC",
"name": "BscMI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Bacillus species 4231",
"methylation_site": ""
},
"taii": {
"target_site": "ACGT^",
"name": "TaiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Jurgelyte, R., Maneliene, Z., Kairaitiene, R., Trinkunaite, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "MaeII",
"organism": "Thermus aquaticus cs1-331",
"methylation_site": ""
},
"bspmai": {
"target_site": "CTGCA^G",
"name": "BspMAI",
"suppliers": [],
"source": "P.M. Skowron",
"references": [
"Skowron, P.M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus stearothermophilus MA",
"methylation_site": ""
},
"stai": {
"target_site": "CCGCGG",
"name": "StaI",
"suppliers": [],
"source": "ATCC 27649",
"references": [
"Klein, S., Roberts, R.J., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces takatoensis",
"methylation_site": ""
},
"bseqi": {
"target_site": "GG^CC",
"name": "BseQI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "HaeIII",
"organism": "Bacillus species Q",
"methylation_site": ""
},
"llaai": {
"target_site": "^GATC",
"name": "LlaAI",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Josephsen, J., Nyengaard, N.R., Vogensen, F.K., Madsen, A., International Patent Office, 1996.",
"Nyengaard, N., Vogensen, F.K., Josephsen, J., (1993) Gene, vol. 136, pp. 371-372."
],
"prototype": "MboI",
"organism": "Lactococcus lactis subsp. cremoris W9",
"methylation_site": ""
},
"uba1249i": {
"target_site": "GGWCC",
"name": "Uba1249I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1249",
"methylation_site": ""
},
"rrbi": {
"target_site": "?",
"name": "RrbI",
"suppliers": [],
"source": "J. Chirikjian",
"references": [
"LeBon, J.M., LeBon, T., Blakesley, R., Chirikjian, J., Unpublished observations."
],
"prototype": "",
"organism": "Rhodospirillum rubrum",
"methylation_site": ""
},
"ngoaviii": {
"target_site": "(12/14)GACNNNNNTGA(13/11)",
"name": "NgoAVIII",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Lewis, L.A. et al., Unpublished observations.",
"O'Driscoll, J., Wilson, G.G., Unpublished observations.",
"Wilson, G., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(6)"
},
"vcho24i": {
"target_site": "?",
"name": "VchO24I",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "",
"organism": "Vibrio cholerae 14438-62",
"methylation_site": ""
},
"asuii": {
"target_site": "TT^CGAA",
"name": "AsuII",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "CCAP 1403/4b",
"references": [
"de Waard, A., Duyvesteyn, M., (1980) Arch. Microbiol., vol. 128, pp. 242-247."
],
"prototype": "",
"organism": "Anabaena subcylindrica",
"methylation_site": ""
},
"bspdi": {
"target_site": "AT^CGAT",
"name": "BspDI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 595",
"references": [
"Kong, H., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species",
"methylation_site": ""
},
"uba1137i": {
"target_site": "ATCGAT",
"name": "Uba1137I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1137",
"methylation_site": ""
},
"bsrdi": {
"target_site": "GCAATG(2/0)",
"name": "BsrDI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Xu, S.Y., Zhu, Z., Zhang, P., Chan, S.H., Samuelson, J.C., Xiao, J., Ingalls, D., Wilson, G.G., (2007) Nucleic Acids Res., vol. 35, pp. 4608-4618."
],
"prototype": "",
"organism": "Bacillus stearothermophilus D70",
"methylation_site": ""
},
"pspei": {
"target_site": "G^GTNACC",
"name": "PspEI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BstEII",
"organism": "Pseudomonas species E",
"methylation_site": ""
},
"bli41i": {
"target_site": "AT^CGAT",
"name": "Bli41I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Hachaturyan, A.A., Afrikyan, E.K., (1989) Biol. J. Armenia, vol. 42, pp. 969-972."
],
"prototype": "ClaI",
"organism": "Bacillus licheniformis 41",
"methylation_site": ""
},
"aor13hi": {
"target_site": "T^CCGGA",
"name": "Aor13HI",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Hikita, T., Yanagidani, S., Nomura, Y., Kishimoto, N., Tano, T., Tanaka, H., (1993) Biosci. Biotechnol. Biochem., vol. 57, pp. 1716-1721."
],
"prototype": "BspMII",
"organism": "Acidiphilium organovorum 13H",
"methylation_site": ""
},
"hpyf51ii": {
"target_site": "ACNGT",
"name": "HpyF51II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL51",
"methylation_site": ""
},
"acs1422i": {
"target_site": "GTCGAC",
"name": "Acs1422I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Hamafuku, H., Kotani, H., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "SalI",
"organism": "Acidobacterium capsulatum 1422",
"methylation_site": ""
},
"pun14627i": {
"target_site": "TGC^GCA",
"name": "Pun14627I",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Waleron, K., Swiatek, W., Biedrzycka, I., Podhajska, A.J., (2001) FEMS Microbiol. Lett., vol. 198, pp. 135-140."
],
"prototype": "MstI",
"organism": "Phormidium uncinatum 1462/7",
"methylation_site": ""
},
"bsthi": {
"target_site": "CTCGAG",
"name": "BstHI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus stearothermophilus H1",
"methylation_site": ""
},
"apc202i": {
"target_site": "?",
"name": "Apc202I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Aphanizomenon species 202",
"methylation_site": ""
},
"bspxi": {
"target_site": "AT^CGAT",
"name": "BspXI",
"suppliers": [],
"source": "D. Dupret",
"references": [
"Zieger, M., Patillon, M., Roizes, G., Lerouge, T., Dupret, D., Jeltsch, J.M., (1987) Nucleic Acids Res., vol. 15, pp. 3919."
],
"prototype": "ClaI",
"organism": "Bacillus sphaericus X",
"methylation_site": ""
},
"m.stylt2dam": {
"target_site": "GATC",
"name": "M.StyLT2Dam",
"suppliers": [],
"source": "ATCC 700720",
"references": [
"McClelland, M. et al., (2001) Nature, vol. 413, pp. 852-856.",
"Pucciarelli, M.G., Prieto, A.I., Casadesus, J., Garcia-Del Portillo, F., (2002) Microbiology, vol. 148, pp. 1171-1182."
],
"prototype": "MboI",
"organism": "Salmonella typhimurium LT2",
"methylation_site": "?(6)"
},
"nlii": {
"target_site": "CYCGRG",
"name": "NliI",
"suppliers": [],
"source": "A. de Waard",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaI",
"organism": "Nostoc linckia",
"methylation_site": ""
},
"ssp5230i": {
"target_site": "GACGT^C",
"name": "Ssp5230I",
"suppliers": [],
"source": "P. Westermann",
"references": [
"Knoblich, I.M., Sellmann, E., Kaluza, K., Frey, B., Auer, J., Schmitz, G.G., Westermann, P., (1992) Nucleic Acids Res., vol. 20, pp. 2378."
],
"prototype": "AatII",
"organism": "Streptomyces species 5230",
"methylation_site": ""
},
"uba1210i": {
"target_site": "GGCC",
"name": "Uba1210I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1210",
"methylation_site": ""
},
"psuni": {
"target_site": "CRCCGGYG",
"name": "PsuNI",
"suppliers": [],
"source": "NEB 1317",
"references": [
"Hingorani, K., Bitinaite, J., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "SgrAI",
"organism": "Pseudomonas stutzeri 1660",
"methylation_site": "?(5)"
},
"bsemi": {
"target_site": "GCAATG(2/0)",
"name": "BseMI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Kazlauskiene, R., Capskaja, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BsrDI",
"organism": "Bacillus stearothermophilus Isl 15-111",
"methylation_site": ""
},
"bsp17i": {
"target_site": "CTGCAG",
"name": "Bsp17I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species RFL17",
"methylation_site": ""
},
"m.ecop1dam": {
"target_site": "GATC",
"name": "M.EcoP1Dam",
"suppliers": [],
"source": "N. Sternberg",
"references": [
"Coulby, J.N., Sternberg, N.L., (1988) Gene, vol. 74, pp. 191."
],
"prototype": "MboI",
"organism": "Escherichia coli phage P1",
"methylation_site": "2(6)"
},
"bsh1285i": {
"target_site": "CGRY^CG",
"name": "Bsh1285I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Maneliene, Z., Capskaya, L., Kiuduliene, L., Butkus, V., Unpublished observations."
],
"prototype": "McrI",
"organism": "Bacillus sphaericus RFL1285",
"methylation_site": ""
},
"kpn10i": {
"target_site": "CCWGG",
"name": "Kpn10I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Klebsiella pneumoniae RFL10",
"methylation_site": ""
},
"novii": {
"target_site": "GANTC",
"name": "NovII",
"suppliers": [],
"source": "NRCC 31020",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Neisseria ovis",
"methylation_site": ""
},
"pae15i": {
"target_site": "CTGCAG",
"name": "Pae15I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL15",
"methylation_site": ""
},
"esp16i": {
"target_site": "CGTCTC",
"name": "Esp16I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Esp3I",
"organism": "Erwinia species RFL16",
"methylation_site": ""
},
"naswi": {
"target_site": "GCCGGC",
"name": "NasWI",
"suppliers": [],
"source": "ATCC 14759",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Nocardia asteroides",
"methylation_site": ""
},
"hpyf21ii": {
"target_site": "GTAC",
"name": "HpyF21II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL21",
"methylation_site": ""
},
"ecor25i": {
"target_site": "?",
"name": "EcoR25I",
"suppliers": [],
"source": "ATCC 35344",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR25",
"methylation_site": "?(6)"
},
"uban6ii": {
"target_site": "CYCGRG",
"name": "UbaN6II",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "AvaI",
"organism": "Unidentified bacterium CN12-05, Nicaragua",
"methylation_site": ""
},
"rpai": {
"target_site": "GTYGGAG(11/9)",
"name": "RpaI",
"suppliers": [],
"source": "ATCC BAA-98",
"references": [
"Dwinell, E.A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Rhodopseudomonas palustris CGA009",
"methylation_site": "?(6)"
},
"btkii": {
"target_site": "^GATC",
"name": "BtkII",
"suppliers": [],
"source": "R.R. Azizbekyan",
"references": [
"Azizbekyan, R.R., Rebentish, B.A., Netyksa, E.M., Bychkova, M.A., Bolotin, A.P., (1992) Mol. Gen. Mikrobiol. Virusol., vol. 1-2, pp. 13-15."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis var Kumantoensis",
"methylation_site": ""
},
"aaai": {
"target_site": "C^GGCCG",
"name": "AaaI",
"suppliers": [],
"source": "M. Fukaya",
"references": [
"Tagami, H., Tayama, K., Tohyama, T., Fukaya, M., Okumura, H., Kawamura, Y., Horinouchi, S., Beppu, T., (1988) FEMS Microbiol. Lett., vol. 56, pp. 161-166."
],
"prototype": "XmaIII",
"organism": "Acetobacter aceti ss aceti",
"methylation_site": ""
},
"bmeti": {
"target_site": "TGATCA",
"name": "BmeTI",
"suppliers": [],
"source": "ATCC 53907",
"references": [
"Cue, D., Hong, L., Hanson, R.S., Flickinger, M.C., (1996) Appl. Environ. Microbiol., vol. 62, pp. 1107-1111."
],
"prototype": "BclI",
"organism": "Bacillus methanolicus",
"methylation_site": "3(6)"
},
"ali12258i": {
"target_site": "GGATCC",
"name": "Ali12258I",
"suppliers": [],
"source": "IFO 12258",
"references": [
"Yamada, Y., Sasaki, J., (1984) J. Gen. Appl. Microbiol., vol. 30, pp. 309-312."
],
"prototype": "BamHI",
"organism": "Acetobacter liquefaciens",
"methylation_site": ""
},
"paeci": {
"target_site": "GCATGC",
"name": "PaeCI",
"suppliers": [],
"source": "NRCC 5003",
"references": [
"Earle-Hughes, J., Roberts, R.J., Unpublished observations."
],
"prototype": "SphI",
"organism": "Pseudomonas aeruginosa",
"methylation_site": ""
},
"mth1047i": {
"target_site": "GATC",
"name": "Mth1047I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Microbacterium thermosphactum 1047",
"methylation_site": ""
},
"bstmai": {
"target_site": "GTCTC(1/5)",
"name": "BstMAI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Mikhnenkova, N.A., Kileva, E.V., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsmAI",
"organism": "Bacillus stearothermophilus MA",
"methylation_site": ""
},
"asumbi": {
"target_site": "GATC",
"name": "AsuMBI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Degtyarev, S.Kh., (1998) Biol. Chem., vol. 379, pp. 573-574."
],
"prototype": "MboI",
"organism": "Actinobacillus suis MB",
"methylation_site": ""
},
"eco216i": {
"target_site": "GRGCYC",
"name": "Eco216I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL216",
"methylation_site": ""
},
"ncuii": {
"target_site": "CCCG",
"name": "NcuII",
"suppliers": [],
"source": "ATCC 14688",
"references": [
"Walsh, P., Unpublished observations."
],
"prototype": "Sth132I",
"organism": "Neisseria cuniculi",
"methylation_site": ""
},
"bfmi": {
"target_site": "C^TRYAG",
"name": "BfmI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Capskaja, L., Markauskiene, J., Kiuduliene, L., Butkus, V., Janulaitis, J., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Bacillus firmus S8-336",
"methylation_site": ""
},
"hsp2i": {
"target_site": "GGWCC",
"name": "Hsp2I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Hafnia species RFL2",
"methylation_site": ""
},
"psc18i": {
"target_site": "?",
"name": "Psc18I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species 18",
"methylation_site": ""
},
"ecl136ii": {
"target_site": "GAG^CTC",
"name": "Ecl136II",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Butkus, V., Maneliene, Z., Petrusyte, M., Unpublished observations."
],
"prototype": "SacI",
"organism": "Enterobacter cloacae RFL136",
"methylation_site": ""
},
"rhpi": {
"target_site": "GTCGAC",
"name": "RhpI",
"suppliers": [],
"source": "ATCC 19148",
"references": [
"Hurlin, P., Schildkraut, I., Unpublished observations."
],
"prototype": "SalI",
"organism": "Rhodococcus species",
"methylation_site": ""
},
"bsp48i": {
"target_site": "CCGG",
"name": "Bsp48I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Bacillus species RFL48",
"methylation_site": ""
},
"sati": {
"target_site": "GC^NGC",
"name": "SatI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Kesminiene, A., Maneliene, Z., Petrusyte, M., Capskaja, L., Trinkunaite, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "Fnu4HI",
"organism": "Staphylococcus arlettae RFL 832",
"methylation_site": ""
},
"taq52i": {
"target_site": "G^CWGC",
"name": "Taq52I",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Williams, R.A.D., (1995) Nucleic Acids Res., vol. 23, pp. 4573-4575."
],
"prototype": "TseI",
"organism": "Thermus aquaticus YS52",
"methylation_site": ""
},
"ppu11i": {
"target_site": "YACGTR",
"name": "Ppu11I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BsaAI",
"organism": "Pseudomonas putida RFL11",
"methylation_site": ""
},
"cgli": {
"target_site": "GCSGC",
"name": "CglI",
"suppliers": [],
"source": "ATCC 13032",
"references": [
"Ikeda, M., Nakagawa, S., (2003) Appl. Microbiol. Biotechnol., vol. 62, pp. 99-109.",
"Kalinowski, J. et al., (2003) J. Biotechnol., vol. 104, pp. 5-25.",
"Nakagawa, S., Mizoguchi, H., Ando, S., Hayashi, M., Ochiai, K., Yokoi, H., Tateishi, N., Senoh, A., Ikeda, M., Ozaki, A., Korean Patent Office, 2000.",
"Nakagawa, S., Mizoguchi, H., Ando, S., Hayashi, M., Ochiai, K., Yokoi, H., Tateishi, N., Senoh, A., Ikeda, M., Ozaki, A., US Patent Office, 2008.",
"Nakagawa, S., Mizoguchi, H., Ando, S., Hayashi, M., Ochiai, K., Yokoi, H., Tateishi, N., Senoh, A., Ikeda, M., Ozaki, A., European Patent Office, 2009.",
"Nakagawa, S., Mizoguchi, H., Ando, S., Hayashi, M., Ochiai, K., Yokoi, H., Tateishi, N., Senoh, A., Ikeda, M., Ozaki, A., Unpublished observations.",
"Nakagawa, S., Mizoguchi, H., Ando, S., Hayashi, M., Ochiai, K., Yokoi, H., Tateishi, N., Senoo, A., Ikeda, M., Ozaki, A., Japanese Patent Office, 2002.",
"Pompejus, M., Kroeger, B., Schroeder, H., Zelder, O., Haberhauer, G., Korean Patent Office, 2001.",
"Pompejus, M., Kroeger, B., Schroeder, H., Zelder, O., Haberhauer, G., Korean Patent Office, 2006.",
"Roberts, R.J., Unpublished observations.",
"Schaefer, A., Tauch, A., Droste, N., Kalinowski, J., Puehler, A., Unpublished observations.",
"Schafer, A., Schwarzer, A., Kalinowski, J., Puhler, A., (1994) J. Bacteriol., vol. 176, pp. 7309-7319.",
"Schafer, A., Tauch, A., Droste, N., Puhler, A., Kalinowski, J., (1997) Gene, vol. 203, pp. 95-101.",
"Skoglund, A., Andres, S., Engstrand, L., Unpublished observations.",
"Zelder, O., Schroeder, H., Haberhauer, G., Kroeger, B., Pompejus, M., Japanese Patent Office, 2004."
],
"prototype": "TauI",
"organism": "Corynebacterium glutamicum",
"methylation_site": "?(5)"
},
"bstei": {
"target_site": "?",
"name": "BstEI",
"suppliers": [],
"source": "ATCC 49830",
"references": [
"Meagher, R.B., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus ET",
"methylation_site": ""
},
"ngoaiii": {
"target_site": "CCGC^GG",
"name": "NgoAIII",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Hammond, A.W., Chatterjee, D.K., International Patent Office, 1991.",
"Hammond, A.W., Gerard, G.F., Chatterjee, D.K., (1989) Nucleic Acids Res., vol. 17, pp. 6750.",
"Lewis, L.A. et al., Unpublished observations.",
"Monaci, E., Masignani, V., Pizza, M., Fontana, M.R., Japanese Patent Office, 2004.",
"Roe, B., Song, L., Lin, S.-P., Yuan, X.-L., Clifton, S., Ducey, T., Lewis, L., Dyer, D., Unpublished observations."
],
"prototype": "SacII",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(5)"
},
"hgiei": {
"target_site": "G^GWCC",
"name": "HgiEI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Erdmann, D., (1991) Ph.D. Thesis, Justus-Liebig University, Geissen, W. Germany, pp. 1-204.",
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141."
],
"prototype": "AvaII",
"organism": "Herpetosiphon giganteus Hpg24",
"methylation_site": "?(5)"
},
"pshci": {
"target_site": "CACGTG",
"name": "PshCI",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Kimizuka, F., Kita, A., Matsushita, S., Kudo, Y., Shimada, T., Mise, K., (1996) Biol. Pharm. Bull., vol. 19, pp. 1506-1507."
],
"prototype": "PmaCI",
"organism": "Plesiomonas shigelloides PL50",
"methylation_site": ""
},
"bst31ti": {
"target_site": "GGATC(4/5)",
"name": "Bst31TI",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "BinI",
"organism": "Bacillus stearothermophilus 31T",
"methylation_site": ""
},
"eco1386i": {
"target_site": "GGANNNNNNNNATGC",
"name": "Eco1386I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco377I",
"organism": "Escherichia coli EC1386",
"methylation_site": ""
},
"goxi": {
"target_site": "GGATCC",
"name": "GoxI",
"suppliers": [],
"source": "IAM 1836",
"references": [
"Seurinck, J., van Montagu, M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Gluconobacter oxydans sub. melonogenes",
"methylation_site": ""
},
"hhdi": {
"target_site": "CCWGG",
"name": "HhdI",
"suppliers": [],
"source": "ATCC 29686",
"references": [
"Earle-Hughes, J., Roberts, R.J., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Halomonas halodurans",
"methylation_site": ""
},
"xpai": {
"target_site": "C^TCGAG",
"name": "XpaI",
"suppliers": [],
"source": "ATCC 14180",
"references": [
"Gingeras, T.R., Myers, P.A., Olson, J.A., Hanberg, F.A., Roberts, R.J., (1978) J. Mol. Biol., vol. 118, pp. 113-122."
],
"prototype": "XhoI",
"organism": "Xanthomonas papavericola",
"methylation_site": ""
},
"uur960i": {
"target_site": "GC^NGC",
"name": "Uur960I",
"suppliers": [],
"source": "ATCC 27618",
"references": [
"Cocks, B.G., Finch, L.R., (1987) Int. J. Syst. Bacteriol., vol. 37, pp. 451-453."
],
"prototype": "Fnu4HI",
"organism": "Ureaplasma urealyticum 960",
"methylation_site": ""
},
"bdai": {
"target_site": "(10/12)TGANNNNNNTCA(12/10)",
"name": "BdaI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Zakareviciene, L., Lubys, A., Unpublished observations.",
"Nekrasiene, D., Vitkute, J., Lapcinskaja, S., Capskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Brevundimonas diminuta RFL2",
"methylation_site": ""
},
"tsp45i": {
"target_site": "^GTSAC",
"name": "Tsp45I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "R.J. Sharp",
"references": [
"Raven, N.D.H., Williams, R.A.D., Smith, K.E., Kelly, C.D., Carter, N.D., (1993) Nucleic Acids Res., vol. 21, pp. 4397.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Wayne, J., Holden, M., Xu, S.-Y., (1997) Gene, vol. 202, pp. 83-88."
],
"prototype": "",
"organism": "Thermus species strain YS45",
"methylation_site": "4(6)"
},
"xnii": {
"target_site": "CGATCG",
"name": "XniI",
"suppliers": [],
"source": "ATCC 23390",
"references": [
"Hanberg, F., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Xanthomonas nigromaculans",
"methylation_site": ""
},
"m.taecdnmti": {
"target_site": "?",
"name": "M.TaeCDnmtI",
"suppliers": [],
"source": "O.D. Anderson",
"references": [
"Anderson, O.D., Chao, S., Choi, D.W., Close, T.J., Fenton, R.D., Han, P.S., Hsia, C.C., Kang, Y., Lazo, G.R., Miller, R., Nguyen, H.T., Rausch, C.J., Seaton, C.L., Tong, J.C., Zhang, D., Unpublished observations.",
"Anderson, O.D., Chao, S., Crossman, C., Langridge, P., Lazo, G.R., Pham, J., Rausch, C.J., Sutton, T., Woo, J., Wilson, C., Unpublished observations."
],
"prototype": "",
"organism": "Triticum aestivum Chinese Spring",
"methylation_site": "?(5)"
},
"bsni": {
"target_site": "GG^CC",
"name": "BsnI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 78",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sp. SJN7",
"methylation_site": ""
},
"ssl16215i": {
"target_site": "?",
"name": "Ssl16215I",
"suppliers": [],
"source": "FERM 16215",
"references": [
"Tomita, M., Toyama, K., Hiramatsu, A., Sho, K., Takahashi, T., Japanese Patent Office, 2000."
],
"prototype": "",
"organism": "Streptococcus salivarius subsp. thermophilus",
"methylation_site": ""
},
"bsati": {
"target_site": "TGCGCA",
"name": "BsaTI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "MstI",
"organism": "Bacillus stearothermophilus T1544",
"methylation_site": ""
},
"kfli": {
"target_site": "GG^GWCCC",
"name": "KflI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G320",
"references": [
"Lauciuniene, N., Norgeliene, D., Trinkunaite, L., Vitkute, J., Janulaitis, A., Unpublished observations.",
"Zakareviciene, L., Lubys, A., Unpublished observations."
],
"prototype": "SanDI",
"organism": "Kribbella flavida RFL1",
"methylation_site": ""
},
"sau3239i": {
"target_site": "C^TCGAG",
"name": "Sau3239I",
"suppliers": [],
"source": "CCM 3239",
"references": [
"Gasperik, J., Godany, A., Hostinova, E., Zelinka, J., (1983) Biologia (Bratisl), vol. 38, pp. 315-319.",
"Simbochova, G., Timko, J., Zelinkova, E., Zelinka, J., (1986) Biologia (Bratisl), vol. 41, pp. 357-365.",
"Zelinkova, E., Paulicek, M., Zelinka, J., (1990) FEBS Lett., vol. 271, pp. 147-148."
],
"prototype": "XhoI",
"organism": "Streptomyces aureofaciens",
"methylation_site": "5(6)"
},
"hpaii": {
"target_site": "C^CGG",
"name": "HpaII",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 49669",
"references": [
"Butkus, V., Petrauskiene, L., Maneliene, Z., Klimasauskas, S., Laucys, V., Janulaitis, A., (1987) Nucleic Acids Res., vol. 15, pp. 7091-7102.",
"Chatterjee, D.K., US Patent Office, 1993.",
"Garfin, D.E., Goodman, H.M., (1974) Biochem. Biophys. Res. Commun., vol. 59, pp. 108-116.",
"Kulakauskas, S., Barsomian, J.M., Lubys, A., Roberts, R.J., Wilson, G.G., (1994) Gene, vol. 142, pp. 9-15.",
"Mann, M.B., Smith, H.O., (1977) Nucleic Acids Res., vol. 4, pp. 4211-4221.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Sharp, P.A., Sugden, B., Sambrook, J., (1973) Biochemistry, vol. 12, pp. 3055-3063.",
"Som, S., Friedman, S., (1994) J. Biol. Chem., vol. 269, pp. 25986-25991."
],
"prototype": "",
"organism": "Haemophilus parainfluenzae",
"methylation_site": "2(5)"
},
"rma497ii": {
"target_site": "GATATC",
"name": "Rma497II",
"suppliers": [],
"source": "ITI 497",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"uba1184i": {
"target_site": "CTGCAG",
"name": "Uba1184I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1184",
"methylation_site": ""
},
"ecocki": {
"target_site": "?",
"name": "EcoCKI",
"suppliers": [],
"source": "S.S. Debov",
"references": [
"Uporova, T.M., Nikolskaya, I.I., Rubtsova, E.N., Debov, S.S., (1981) Vestn. Akad. Med. Nauk SSSR, vol. 2, pp. 21-26."
],
"prototype": "",
"organism": "Escherichia coli CK",
"methylation_site": ""
},
"aasi": {
"target_site": "GACNNNN^NNGTC",
"name": "AasI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Kazlauskiene, R., Vaitkevicius, D., Maneliene, Z., Trinkunaite, L., Kiuduliene, L., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "DrdI",
"organism": "Arthrobacter aurescens RFL3",
"methylation_site": ""
},
"bsc217i": {
"target_site": "GATATC",
"name": "Bsc217I",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Bacillus species HK217",
"methylation_site": ""
},
"vpakuthi": {
"target_site": "GGTCTC",
"name": "VpaKutHI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "Eco31I",
"organism": "Vibrio parahaemolyticus TVP1756",
"methylation_site": ""
},
"bstt9i": {
"target_site": "G^GTNACC",
"name": "BstT9I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BstEII",
"organism": "Bacillus stearothermophilus T9",
"methylation_site": ""
},
"avaiii": {
"target_site": "ATGCAT",
"name": "AvaIII",
"suppliers": [],
"source": "ATCC 27893",
"references": [
"Elhai, J., Unpublished observations.",
"Roizes, G., Nardeux, P.C., Monier, R., (1979) FEBS Lett., vol. 104, pp. 39-44.",
"Shimatake, H., Rosenberg, M., Unpublished observations."
],
"prototype": "",
"organism": "Anabaena variabilis",
"methylation_site": ""
},
"ese6i": {
"target_site": "CCGCGG",
"name": "Ese6I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Enterobacter species RFL6",
"methylation_site": ""
},
"bsp81i": {
"target_site": "CTGCAG",
"name": "Bsp81I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species RFL81",
"methylation_site": ""
},
"baniii": {
"target_site": "AT^CGAT",
"name": "BanIII",
"suppliers": [
"Toyobo Biochemicals (9/09)"
],
"source": "IAM 1077",
"references": [
"Kawakami, B., Sasaki, A., Oka, M., Maekawa, Y., (1990) Agric. Biol. Chem., vol. 54, pp. 3227-3233.",
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Nucleic Acids Res., vol. 10, pp. 5747-5752.",
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus aneurinolyticus",
"methylation_site": ""
},
"bsici": {
"target_site": "TT^CGAA",
"name": "BsiCI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"sst12i": {
"target_site": "CTGCA^G",
"name": "Sst12I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Andreeva, I.S., Repin, V.E., Krivopalova, G.N., Russian Patent Office, 1999.",
"Ushakova, T.A., Puchkova, L.I., Gutorov, V.V., Kuvshinov, V.N., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 25-28."
],
"prototype": "PstI",
"organism": "Streptomyces species ST-12",
"methylation_site": ""
},
"bsigi": {
"target_site": "TCCGGA",
"name": "BsiGI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Shaw, P.C., Clark, D.R., Kam, K.M., Mok, Y.K., (1991) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 91, pp. 199."
],
"prototype": "BspMII",
"organism": "Bacillus species",
"methylation_site": ""
},
"m.hinhp1dam": {
"target_site": "GATC",
"name": "M.HinHP1Dam",
"suppliers": [],
"source": "J.J. Scocca",
"references": [
"Esposito, D., Fitzmaurice, W.P., Benjamin, R.C., Goodman, S.D., Waldman, A.S., Socca, J.J., (1996) Nucleic Acids Res., vol. 24, pp. 2360-2368.",
"Piekarowicz, A., Bujnicki, J., (1999) Acta Microbiol. Pol., vol. 48, pp. 123-129."
],
"prototype": "MboI",
"organism": "Haemophilus influenza bacteriophage HP1",
"methylation_site": "2(6)"
},
"finsi": {
"target_site": "GGCC",
"name": "FinSI",
"suppliers": [],
"source": "ATCC 27950",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Flavobacterium indoltheticum",
"methylation_site": ""
},
"acci": {
"target_site": "GT^MKAC",
"name": "AccI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 49823",
"references": [
"Kawakami, B., Hilzheber, C., Nagatomo, M., Oka, M., (1991) Agric. Biol. Chem., vol. 55, pp. 1553-1559.",
"Kawakami, B., Maekawa, Y., Japanese Patent Office, 1991.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Zabeau, M., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Acinetobacter calcoaceticus",
"methylation_site": "5(6)"
},
"sflhk2731i": {
"target_site": "CCWGG",
"name": "SflHK2731I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 2731",
"methylation_site": ""
},
"afii": {
"target_site": "CCNNNNN^NNGG",
"name": "AfiI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 24",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "BsiYI",
"organism": "Anoxybacillus flavithermus",
"methylation_site": ""
},
"pae8i": {
"target_site": "CTGCAG",
"name": "Pae8I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL8",
"methylation_site": ""
},
"m.ecot1dam": {
"target_site": "GATC",
"name": "M.EcoT1Dam",
"suppliers": [],
"source": "M. Schweiger",
"references": [
"Roberts, M.D., Martin, N.L., Kropinski, A.M., (2004) Virology, vol. 318, pp. 245-266.",
"Scherzer, E., Auer, B., Schweiger, M., (1987) J. Biol. Chem., vol. 262, pp. 15225-15231.",
"Schneider-Scherzer, E., Auer, B., de Groot, E.J., Schweiger, M., (1990) J. Biol. Chem., vol. 265, pp. 6086-6091."
],
"prototype": "MboI",
"organism": "Escherichia coli phage T1",
"methylation_site": "2(6)"
},
"vspi": {
"target_site": "AT^TAAT",
"name": "VspI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Prikhodko, E.A., Rechkunova, N.I., Prikhodko, G.G., Krasnykh, V.N., (1995) Gene, vol. 157, pp. 65-66.",
"Degtyarev, S.Kh., Prikhodko, E.A., Prikhodko, G.G., Krasnykh, V.N., (1993) Nucleic Acids Res., vol. 21, pp. 2015.",
"Degtyarev, S.Kh., Repin, V.E., Rechkunova, N.I., Tchigikov, V.E., Malygin, E.G., Mikhajlov, V.V., Rasskazov, V.A., (1987) Bioorg. Khim., vol. 13, pp. 420-421."
],
"prototype": "",
"organism": "Vibrio species 343",
"methylation_site": "5(6)"
},
"bsp104i": {
"target_site": "TTCGAA",
"name": "Bsp104I",
"suppliers": [],
"source": "ITI 104",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"ctyi": {
"target_site": "GATC",
"name": "CtyI",
"suppliers": [],
"source": "DSM 568",
"references": [
"Richards, D.F., Linnett, P.E., Oultram, J.D., Young, M., (1988) J. Gen. Microbiol., vol. 134, pp. 3151-3157."
],
"prototype": "MboI",
"organism": "Clostridium thermohydrosulfuricum",
"methylation_site": ""
},
"dsavi": {
"target_site": "GTMKAC",
"name": "DsaVI",
"suppliers": [],
"source": "A.E. Walsby",
"references": [
"Laue, F., Evans, L.R., Jarsch, M., Brown, N.L., Kessler, C., (1991) Gene, vol. 97, pp. 87-95."
],
"prototype": "AccI",
"organism": "Dactylococcopsis salina",
"methylation_site": ""
},
"pinbi": {
"target_site": "ATGCA^T",
"name": "PinBI",
"suppliers": [],
"source": "CCAP 1462/9",
"references": [
"Piechula, S., Kur, J., Woszczyk, J., Podhajska, A.J., (1995) Gene, vol. 157, pp. 315-316."
],
"prototype": "AvaIII",
"organism": "Phormidium inundatum",
"methylation_site": ""
},
"bst11i": {
"target_site": "ACTGG(1/-1)",
"name": "Bst11I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BsrI",
"organism": "Bacillus stearothermophilus 11",
"methylation_site": ""
},
"hpyiv": {
"target_site": "GANTC",
"name": "HpyIV",
"suppliers": [],
"source": "ATCC 49503",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori 60190",
"methylation_site": ""
},
"m.bamhii": {
"target_site": "GGATCC",
"name": "M.BamHII",
"suppliers": [],
"source": "ATCC 49763",
"references": [
"Connaughton, J.F., Vanek, P.G., Chirikjian, J.G., (1988) J. Cell Biol., vol. 107, pp. 535a."
],
"prototype": "BamHI",
"organism": "Bacillus amyloliquefaciens H",
"methylation_site": ""
},
"hpyii": {
"target_site": "GAAGA",
"name": "HpyII",
"suppliers": [],
"source": "ATCC 49503",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "MboII",
"organism": "Helicobacter pylori 60190",
"methylation_site": ""
},
"apeai": {
"target_site": "GCCGGC",
"name": "ApeAI",
"suppliers": [],
"source": "ATCC 15387",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Aquaspirillum peregrinum",
"methylation_site": ""
},
"bmgai": {
"target_site": "GKGCMC",
"name": "BmgAI",
"suppliers": [],
"source": "NEB 1355",
"references": [
"Le, T.K.T., Vu, H.N., Vu, T.K.L., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "BseSI",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"nsp152i": {
"target_site": "?",
"name": "Nsp152I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Nostoc species 152",
"methylation_site": ""
},
"bsph226i": {
"target_site": "TCCGGA",
"name": "BspH226I",
"suppliers": [],
"source": "ITI 226",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Bacillus species",
"methylation_site": ""
},
"uban4i": {
"target_site": "GATATC",
"name": "UbaN4I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "EcoRV",
"organism": "Unidentified bacterium T1-03, Nicaragua",
"methylation_site": ""
},
"m.dreldnmt1": {
"target_site": "?",
"name": "M.DreLDnmt1",
"suppliers": [],
"source": "E. Li",
"references": [
"Martin, C.C., Laforest, L., Akimenko, M.-A., Ekker, M., Unpublished observations.",
"Mhanni, A.A., Yoder, J.A., Dubesky, C., McGowan, R.A., (2001) Genesis, vol. 30, pp. 213-219."
],
"prototype": "",
"organism": "Danio rerio",
"methylation_site": "?(5)"
},
"hpyf24ii": {
"target_site": "CTNAG",
"name": "HpyF24II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL24",
"methylation_site": ""
},
"cfr8i": {
"target_site": "GGNCC",
"name": "Cfr8I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL8",
"methylation_site": ""
},
"cfr32i": {
"target_site": "AAGCTT",
"name": "Cfr32I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Citrobacter freundii RFL32",
"methylation_site": ""
},
"bavbi": {
"target_site": "CAG^CTG",
"name": "BavBI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Fitsner, A.B., Anikeitcheva, N.V., Samko, O.T., Kalugin, A.A., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Bacillus alvei B",
"methylation_site": ""
},
"erhi": {
"target_site": "C^CWWGG",
"name": "ErhI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Zhilkina, O.A., Rechkunova, N.I., Semenchenko, G.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "StyI",
"organism": "Erwinia rhaponici B9",
"methylation_site": ""
},
"eco97i": {
"target_site": "GGTCTC",
"name": "Eco97I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL97",
"methylation_site": ""
},
"bcosi": {
"target_site": "CTCTTC",
"name": "BcoSI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "Ksp632I",
"organism": "Bacillus coagulans S",
"methylation_site": ""
},
"bfuci": {
"target_site": "^GATC",
"name": "BfuCI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1441",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations.",
"Samuelson, J.C., Luo, J., International Patent Office, 2009.",
"Zhu, Z., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus fusiformis 1226",
"methylation_site": "?(5)"
},
"bth84i": {
"target_site": "GATC",
"name": "Bth84I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. amagiensis",
"methylation_site": ""
},
"mvni": {
"target_site": "CG^CG",
"name": "MvnI",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "M. Thomm",
"references": [
"Thomm, M., Frey, G., Bolton, B.J., Laue, F., Kessler, C., Stetter, K.O., (1988) FEMS Microbiol. Lett., vol. 52, pp. 229-234."
],
"prototype": "FnuDII",
"organism": "Methanococcus vannielii",
"methylation_site": ""
},
"pmei": {
"target_site": "GTTT^AAAC",
"name": "PmeI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 698",
"references": [
"Chang, Z., Morgan, R.D., US Patent Office, 1999.",
"Morgan, R., Zhou, B., US Patent Office, 1993.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Pseudomonas mendocina",
"methylation_site": ""
},
"bani": {
"target_site": "G^GYRCC",
"name": "BanI",
"suppliers": [
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)"
],
"source": "IAM 1077",
"references": [
"Kawakami, B., Maekawa, Y., Japanese Patent Office, 1989.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Maekawa, Y., Kawakami, F., Yasukawa, H., Japanese Patent Office, 1991.",
"Maekawa, Y., Yasukawa, H., Kawakami, B., (1990) J. Biochem. (Tokyo), vol. 107, pp. 645-649.",
"Schildkraut, I., Lynch, J., Morgan, R., (1987) Nucleic Acids Res., vol. 15, pp. 5492.",
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Nucleic Acids Res., vol. 10, pp. 5747-5752."
],
"prototype": "HgiCI",
"organism": "Bacillus aneurinolyticus",
"methylation_site": "?(5)"
},
"mspbi": {
"target_site": "GATC",
"name": "MspBI",
"suppliers": [],
"source": "J.N. Reeve",
"references": [
"Morgan, R., Reeve, J., Krzycki, J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Methanobrevibacter species MMY",
"methylation_site": ""
},
"ecopi": {
"target_site": "AGACC(25/27)",
"name": "EcoPI",
"suppliers": [],
"source": "K. Murray",
"references": [
"Bachi, B., Reiser, J., Pirrotta, V., (1979) J. Mol. Biol., vol. 128, pp. 143-163.",
"Brockes, J.P., Brown, P.R., Murray, K., (1972) Biochem. J., vol. 127, pp. 1-10.",
"Haberman, A., (1974) J. Mol. Biol., vol. 89, pp. 545-563.",
"Haberman, A., Heywood, J., Meselson, M., (1972) Proc. Natl. Acad. Sci. U. S. A., vol. 69, pp. 3138-3141.",
"Hattman, S., Brooks, J.E., Masurekar, M., (1978) J. Mol. Biol., vol. 126, pp. 367-380.",
"Krishnamurthy, V., Rao, D.N., (1994) Biochem. Mol. Biol. Int., vol. 32, pp. 623-632.",
"Lobocka, M.B., Rose, D.J., Rusin, M., Plunkett, G. III, Samojedny, A., Lehnherr, H., Yarmolinsky, M.B., Blattner, F.R., (2004) J. Bacteriol., vol. 186, pp. 7032-7068."
],
"prototype": "",
"organism": "Escherichia coli (PI)",
"methylation_site": "3(6)"
},
"bsimi": {
"target_site": "T^CCGGA",
"name": "BsiMI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Bacillus species",
"methylation_site": ""
},
"nspv": {
"target_site": "TT^CGAA",
"name": "NspV",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "ATCC 29411",
"references": [
"Bakker, D., Schouten, J.P., Unpublished observations.",
"Reaston, J., Duyvesteyn, M.G.C., de Waard, A., (1982) Gene, vol. 20, pp. 103-110.",
"Ueno, T., Ito, H., Kotani, H., Japanese Patent Office, 1993.",
"Ueno, T., Ito, H., Kotani, H., Kimizuka, F., Nakajima, K., (1993) Nucleic Acids Res., vol. 21, pp. 3899."
],
"prototype": "AsuII",
"organism": "Nostoc species C",
"methylation_site": ""
},
"mva16i": {
"target_site": "TTCGAA",
"name": "Mva16I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Shcheglova, I.K., Russian Patent Office, 1994."
],
"prototype": "AsuII",
"organism": "Micrococcus varians VKPM B-5942",
"methylation_site": ""
},
"ppu20i": {
"target_site": "GRGCYC",
"name": "Ppu20I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Pseudomonas putida RFL20",
"methylation_site": ""
},
"ksphk15i": {
"target_site": "YGGCCR",
"name": "KspHK15I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Klebsiella species N15",
"methylation_site": ""
},
"nlasi": {
"target_site": "CCGCGG",
"name": "NlaSI",
"suppliers": [],
"source": "NRCC 31016",
"references": [
"Camp, R., Visentin, L.P., Unpublished observations."
],
"prototype": "SacII",
"organism": "Neisseria lactamica",
"methylation_site": ""
},
"m.rnodnmt1": {
"target_site": "?",
"name": "M.RnoDnmt1",
"suppliers": [],
"source": "S. Kohsaka",
"references": [
"Deng, J., Szyf, M., (1998) J. Biol. Chem., vol. 273, pp. 22869-22872.",
"Ohsawa, K., Imai, Y., Ito, D., Kohsaka, S., (1996) J. Neurochem., vol. 67, pp. 89-97."
],
"prototype": "",
"organism": "Rattus norvegicus",
"methylation_site": "?(5)"
},
"alei": {
"target_site": "CACNN^NNGTG",
"name": "AleI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1374",
"references": [
"Degtyarev, S.K., Unpublished observations.",
"Wilson, G.G., Lunnen, K.D., Unpublished observations."
],
"prototype": "OliI",
"organism": "Aureobacterium liquefaciens",
"methylation_site": ""
},
"eaci": {
"target_site": "GGATC(4/5)",
"name": "EacI",
"suppliers": [],
"source": "DSM 3953",
"references": [
"Graentzdoerffer, A., Lindenstrauss, U., Pich, A., Andreesen, J.R., German Patent Office, 2002.",
"Graentzdoerffer, A., Lindenstrauss, U., Pich, A., Andreesen, J.R., German Patent Office, 2002."
],
"prototype": "BinI",
"organism": "Eubacterium acidaminophilum",
"methylation_site": ""
},
"bspj64i": {
"target_site": "GATC",
"name": "BspJ64I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL64",
"methylation_site": ""
},
"cvimi": {
"target_site": "RGCY",
"name": "CviMI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Agarwal, K., Unpublished observations."
],
"prototype": "CviJI",
"organism": "Chlorella strain NC64A (IL-2A)",
"methylation_site": ""
},
"hpyc1i": {
"target_site": "CCATC(4/5)",
"name": "HpyC1I",
"suppliers": [],
"source": "J.-T. Wang",
"references": [
"Lin, T.L., Shun, C.T., Chang, K.C., Wang, J.T., (2004) J. Biol. Chem., vol. 279, pp. 11156-11162.",
"Wang, J.-T., Lin, T.-L., US Patent Office, 2007."
],
"prototype": "BccI",
"organism": "Helicobacter pylori",
"methylation_site": ""
},
"rme21i": {
"target_site": "AT^CGAT",
"name": "Rme21I",
"suppliers": [],
"source": "I.S. Andreeva",
"references": [
"Andreeva, I.S., Afinogenova, G.N., Lebedev, L.R., Pustoshilova, N.M., Gordienko, N.Ya., Maistrenko, G.G., (1991) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 30-31.",
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "ClaI",
"organism": "Rhizobium meliloti",
"methylation_site": ""
},
"nanii": {
"target_site": "GATC",
"name": "NanII",
"suppliers": [],
"source": "ATCC 19573",
"references": [
"Dingman, C., Schildkraut, I., Unpublished observations."
],
"prototype": "DpnI",
"organism": "Neisseria animalis",
"methylation_site": ""
},
"ubaf13i": {
"target_site": "GAGNNNNNNCTGG",
"name": "UbaF13I",
"suppliers": [],
"source": "Fermentas G334",
"references": [
"Vitkute, J., Lauciuniene, N., Riauba, L., Norgeliene, D., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Unidentified bacterium F13",
"methylation_site": ""
},
"slei": {
"target_site": "^CCWGG",
"name": "SleI",
"suppliers": [],
"source": "PCC 1402-1",
"references": [
"Piechula, S., Skowron, P.M., Piatyszek, M., Podhajska, A.J., (1991) Nucleic Acids Res., vol. 19, pp. 2782."
],
"prototype": "EcoRII",
"organism": "Synechococcus leopoliensis",
"methylation_site": ""
},
"asp202a1i": {
"target_site": "?",
"name": "Asp202A1I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Anabaena species 202A1",
"methylation_site": ""
},
"vpak11bi": {
"target_site": "G^GWCC",
"name": "VpaK11BI",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "S. Matsushita",
"references": [
"Ueno, H., Kita, A., Miyahara, M., Ishiwata, N., Mise, K., Kato, I., Ishino, Y., (1997) Biosci. Biotechnol. Biochem., vol. 61, pp. 2129-2130."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus 1743-1",
"methylation_site": ""
},
"bspai": {
"target_site": "^GATC",
"name": "BspAI",
"suppliers": [],
"source": "R. Mullings",
"references": [
"Mullings, R., Evans, L.R., Brown, N.L., (1986) FEMS Microbiol. Lett., vol. 37, pp. 237-240."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus JL4B",
"methylation_site": ""
},
"scoai": {
"target_site": "CTGCAG",
"name": "ScoAI",
"suppliers": [],
"source": "ATCC 25444",
"references": [
"Earle-Hughes, J., Roberts, R.J., Unpublished observations."
],
"prototype": "PstI",
"organism": "Streptomyces corchorusii",
"methylation_site": ""
},
"mvri": {
"target_site": "CGAT^CG",
"name": "MvrI",
"suppliers": [
"Bangalore Genei (1/11)"
],
"source": "P. Babu",
"references": [
"Shroff, D., Damodaran, B., Shankar, A., Chandrashekaran, S., Babu, J., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Micrococcus varians",
"methylation_site": ""
},
"eco129i": {
"target_site": "GGTCTC",
"name": "Eco129I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL129",
"methylation_site": ""
},
"mssi": {
"target_site": "GTTT^AAAC",
"name": "MssI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Zutautiene, D., Maneliene, Z., Norgeliene, D., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "PmeI",
"organism": "Methylobacterium species Dd 5-732",
"methylation_site": ""
},
"uban17i": {
"target_site": "AAGCTT",
"name": "UbaN17I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Huete-Perez, J.A., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Unidentified bacterium HSJ-16-1-G, Nicaragua",
"methylation_site": ""
},
"sinhi": {
"target_site": "GGWCC",
"name": "SinHI",
"suppliers": [],
"source": "S. Horiuchi",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Salmonella infantis 85166",
"methylation_site": ""
},
"uba1098i": {
"target_site": "GGATCC",
"name": "Uba1098I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1098",
"methylation_site": ""
},
"bme361i": {
"target_site": "GG^CC",
"name": "Bme361I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "HaeIII",
"organism": "Bacillus megaterium 361",
"methylation_site": ""
},
"bmaci": {
"target_site": "CGATCG",
"name": "BmaCI",
"suppliers": [],
"source": "ATCC 7069",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Bacillus macerans",
"methylation_site": ""
},
"vpak56ai": {
"target_site": "?",
"name": "VpaK56AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "",
"organism": "Vibrio parahaemolyticus TVP1744",
"methylation_site": ""
},
"xcyi": {
"target_site": "C^CCGGG",
"name": "XcyI",
"suppliers": [],
"source": "C.I. Kado",
"references": [
"Froman, B.E., Tait, R.C., Kado, C.I., Rodriguez, R.L., (1984) Gene, vol. 28, pp. 331-335.",
"Withers, B.E., Ambroso, L.A., Dunbar, J.C., (1992) Nucleic Acids Res., vol. 20, pp. 6267-6273."
],
"prototype": "SmaI",
"organism": "Xanthomonas cyanopsidis 13D5",
"methylation_site": "2(4)"
},
"uba1431i": {
"target_site": "TGATCA",
"name": "Uba1431I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BclI",
"organism": "Unidentified bacterium RFL1431",
"methylation_site": ""
},
"bsueii": {
"target_site": "CGCG",
"name": "BsuEII",
"suppliers": [],
"source": "IAM 1231",
"references": [
"Gaido, M.L., Prostko, C.R., Strobl, J.S., (1988) J. Biol. Chem., vol. 263, pp. 4832-4836.",
"Qiang, B.-Q., Schildkraut, I., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Bacillus subtilis",
"methylation_site": "1(5)"
},
"eco185i": {
"target_site": "GGTCTC",
"name": "Eco185I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL185",
"methylation_site": ""
},
"uba1174i": {
"target_site": "GGCC",
"name": "Uba1174I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1174",
"methylation_site": ""
},
"bsp146i": {
"target_site": "GTGCAC",
"name": "Bsp146I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ApaLI",
"organism": "Bacillus species RFL146",
"methylation_site": ""
},
"mel5oi": {
"target_site": "GATC",
"name": "Mel5OI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2004) FEMS Microbiol. Lett., vol. 236, pp. 91-95.",
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193."
],
"prototype": "MboI",
"organism": "Megasphaera elsedenii 5O",
"methylation_site": ""
},
"asp54i": {
"target_site": "?",
"name": "Asp54I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Anabaena species 54",
"methylation_site": ""
},
"bsh45i": {
"target_site": "GWGCW^C",
"name": "Bsh45I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Serov, G.D., Repin, V.E., Puchkova, L.E., Kolesnikov, V.A., Russian Patent Office, 1994."
],
"prototype": "HgiAI",
"organism": "Bacillus sphaericus 45",
"methylation_site": ""
},
"nmuaii": {
"target_site": "GGWCC",
"name": "NmuAII",
"suppliers": [],
"source": "A. de Waard",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaII",
"organism": "Nostoc muscorum M-131-G",
"methylation_site": ""
},
"llabiii": {
"target_site": "?",
"name": "LlaBIII",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Kong, J., Matvienko, N., Josephsen, J., Unpublished observations."
],
"prototype": "",
"organism": "Lactococcus lactis subsp. cremoris W56",
"methylation_site": "?(6)"
},
"ddeii": {
"target_site": "CTCGAG",
"name": "DdeII",
"suppliers": [],
"source": "NCIB 83120",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Desulfovibrio desulfuricans Norway strain",
"methylation_site": ""
},
"palai": {
"target_site": "GG^CGCGCC",
"name": "PalAI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AscI",
"organism": "Pseudomonas alcaligenes BS17",
"methylation_site": ""
},
"hpyf66i": {
"target_site": "GGNCC",
"name": "HpyF66I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Helicobacter pylori RFL66",
"methylation_site": ""
},
"uba1158i": {
"target_site": "AGTACT",
"name": "Uba1158I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Unidentified bacterium RFL1158",
"methylation_site": ""
},
"sau14i": {
"target_site": "GGNCC",
"name": "Sau14I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Staphylococcus aureus RFL14",
"methylation_site": ""
},
"bepi": {
"target_site": "CG^CG",
"name": "BepI",
"suppliers": [],
"source": "P. Venetianer",
"references": [
"Kupper, D., Zhou, J.-G., Kiss, A., Venetianer, P., (1988) Gene, vol. 74, pp. 33.",
"Kupper, D., Zhou, J.-G., Venetianer, P., Kiss, A., (1989) Nucleic Acids Res., vol. 17, pp. 1077-1088.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Venetianer, P., Komocsin, M., Orosz, A., Hungarian Patent Office, 1990.",
"Venetianer, P., Orosz, A., (1988) Nucleic Acids Res., vol. 16, pp. 350."
],
"prototype": "FnuDII",
"organism": "Brevibacterium epidermidis",
"methylation_site": "1(5)"
},
"uth549i": {
"target_site": "GGCC",
"name": "Uth549I",
"suppliers": [],
"source": "FZ 549",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium",
"methylation_site": ""
},
"esassi": {
"target_site": "GACCAC",
"name": "EsaSSI",
"suppliers": [],
"source": "J.C. Venter",
"references": [
"Morgan, R.D., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008.",
"Venter, J.C. et al., (2004) Science, vol. 304, pp. 66-74."
],
"prototype": "",
"organism": "Environmental sample Sargasso Sea",
"methylation_site": "?(6)"
},
"bpu14i": {
"target_site": "TT^CGAA",
"name": "Bpu14I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus pumilus 14",
"methylation_site": ""
},
"eco1432i": {
"target_site": "CCANNNNNNNCTTC",
"name": "Eco1432I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco646I",
"organism": "Escherichia coli EC1432",
"methylation_site": ""
},
"bsu537i": {
"target_site": "GGTCTC(1/5)",
"name": "Bsu537I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Ra, S.R., Kim, H.S., Han, M.G., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Bacillus subtilis 537",
"methylation_site": ""
},
"aspai": {
"target_site": "G^GTNACC",
"name": "AspAI",
"suppliers": [],
"source": "N.L. Brown",
"references": [
"Whitehead, P.R., Brown, N.L., (1981) Biochem. Soc. Trans., vol. 9, pp. 272P."
],
"prototype": "BstEII",
"organism": "Alcaligenes species",
"methylation_site": ""
},
"bsp44ii": {
"target_site": "GGCC",
"name": "Bsp44II",
"suppliers": [],
"source": "ITI 44",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus species",
"methylation_site": ""
},
"bbvaii": {
"target_site": "AT^CGAT",
"name": "BbvAII",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Anikeitcheva, N.V., Samko, O.T., Choroshoutina, E.B., Kalugin, A.A., Fitsner, A.B., Unpublished observations.",
"Sokolov, N.N., Samko, O.T., Anikeitcheva, N.V., Kalugin, A.A., Khoroshutina, E.B., Plutalov, O.V., Birikh, K.R., Berlin, Y.A., (1994) Bioorg. Khim., vol. 20, pp. 1334-1341."
],
"prototype": "ClaI",
"organism": "Bacillus brevis A",
"methylation_site": ""
},
"bmri": {
"target_site": "ACTGGG(5/4)",
"name": "BmrI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1067",
"references": [
"Bao, Y., Higgins, L., Zhang, P., Chan, S.H., Laget, S., Sweeney, S., Lunnen, K., Xu, S.Y., (2008) Protein Expr. Purif., vol. 58, pp. 42-52.",
"Krotee, S., Ganatra, M., Unpublished observations.",
"Le, T.K.T., Morgan, R., Unpublished observations.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "BfiI",
"organism": "Bacillus megaterium GC subgroup A",
"methylation_site": ""
},
"hpyf50i": {
"target_site": "GTNNAC",
"name": "HpyF50I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MjaIV",
"organism": "Helicobacter pylori RFL50",
"methylation_site": ""
},
"eco152i": {
"target_site": "GCGCGC",
"name": "Eco152I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Escherichia coli RFL152",
"methylation_site": ""
},
"fnudii": {
"target_site": "CG^CG",
"name": "FnuDII",
"suppliers": [],
"source": "M. Smith",
"references": [
"Lui, A.C.P., McBride, B.C., Vovis, G.F., Smith, M., (1979) Nucleic Acids Res., vol. 6, pp. 1-15.",
"Murray, I.A., Unpublished observations.",
"Qiang, B.-Q., McClelland, M., Poddar, S., Spokauskas, A., Nelson, M., (1990) Gene, vol. 88, pp. 101-105."
],
"prototype": "",
"organism": "Fusobacterium nucleatum D",
"methylation_site": "1(5)"
},
"pglii": {
"target_site": "?",
"name": "PglII",
"suppliers": [],
"source": "J.V. Leary",
"references": [
"Leary, J.V., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas glycinae",
"methylation_site": ""
},
"sthki": {
"target_site": "GGTACC",
"name": "SthKI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY228",
"methylation_site": ""
},
"hpyf40ii": {
"target_site": "TCGA",
"name": "HpyF40II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL40",
"methylation_site": ""
},
"alw21i": {
"target_site": "GWGCW^C",
"name": "Alw21I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Jagelavicius, M., Bitinaite, J., Maneliene, Z., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Acinetobacter lwoffi RFL21",
"methylation_site": ""
},
"erhb9ii": {
"target_site": "C^CWWGG",
"name": "ErhB9II",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "StyI",
"organism": "Erwinia rhaponici B9",
"methylation_site": ""
},
"bspwi": {
"target_site": "GCNNNNN^NNGC",
"name": "BspWI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Unpublished observations."
],
"prototype": "MwoI",
"organism": "Bacillus species 3456",
"methylation_site": ""
},
"bsp132i": {
"target_site": "GGWCC",
"name": "Bsp132I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus species RFL132",
"methylation_site": ""
},
"pfai": {
"target_site": "GATC",
"name": "PfaI",
"suppliers": [],
"source": "M. Van Montagu",
"references": [
"Van Montagu, M., Sciaky, D., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Pseudomonas facilis",
"methylation_site": ""
},
"ecobi": {
"target_site": "TGANNNNNNNNTGCT",
"name": "EcoBI",
"suppliers": [],
"source": "W. Arber",
"references": [
"Eskin, B., Linn, S., (1972) J. Biol. Chem., vol. 247, pp. 6183-6191.",
"Kuhnlein, U., Arber, W., (1972) J. Mol. Biol., vol. 63, pp. 9-19.",
"Kuhnlein, U., Linn, S., Arber, W., (1969) Proc. Natl. Acad. Sci. U. S. A., vol. 63, pp. 556-562.",
"Lautenberger, J.A., Edgell, M.H., Hutchison, C.A. III, (1979) J. Mol. Biol., vol. 131, pp. 871-875.",
"Lautenberger, J.A., Kan, N.C., Lackey, D., Linn, S., Edgell, M.H., Hutchison, C.A. III, (1978) Proc. Natl. Acad. Sci. U. S. A., vol. 75, pp. 2271-2275.",
"Ravetch, J.V., Horiuchi, K., Zinder, N.D., (1978) Proc. Natl. Acad. Sci. U. S. A., vol. 75, pp. 2266-2270.",
"Sommer, R., Schaller, H., (1979) Mol. Gen. Genet., vol. 168, pp. 331-335.",
"van Ormondt, H., Lautenberger, J.A., Linn, S., de Waard, A., (1973) FEBS Lett., vol. 33, pp. 177-180."
],
"prototype": "",
"organism": "Escherichia coli B",
"methylation_site": "3(6),-4(6)"
},
"uba1398i": {
"target_site": "GGATCC",
"name": "Uba1398I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1398",
"methylation_site": ""
},
"ngoai": {
"target_site": "RGCGCY",
"name": "NgoAI",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Hammond, A.W., Chatterjee, D.K., International Patent Office, 1991.",
"Lewis, L.A. et al., Unpublished observations.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Roe, B., Song, L., Lin, S.-P., Yuan, X.-L., Clifton, S., Ducey, T., Lewis, L., Dyer, D., Unpublished observations.",
"Roe, B.A., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(5)"
},
"ecoprri": {
"target_site": "CCANNNNNNNRTGC",
"name": "EcoprrI",
"suppliers": [],
"source": "T.A. Bickle",
"references": [
"Levitz, R., Chapman, D., Amitsur, M., Green, R., Snyder, L., Kaufmann, G., (1990) EMBO J., vol. 9, pp. 1383-1389.",
"Tyndall, C., Meister, J., Bickle, T.A., (1994) J. Mol. Biol., vol. 237, pp. 266-274."
],
"prototype": "",
"organism": "Escherichia coli",
"methylation_site": "?(6)"
},
"rruai": {
"target_site": "?",
"name": "RruAI",
"suppliers": [],
"source": "D. Yoch",
"references": [
"Hamablet, L., Meyertons, J.L., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Rhodospirillum rubrum 68",
"methylation_site": ""
},
"bspb2i": {
"target_site": "?",
"name": "BspB2I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh., (1990) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 17-18."
],
"prototype": "",
"organism": "Bacillus species B2I",
"methylation_site": ""
},
"llabi": {
"target_site": "C^TRYAG",
"name": "LlaBI",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Nyengaard, N., Vogensen, F.K., Josephsen, J., (1993) Gene, vol. 136, pp. 371-372.",
"Nyengaard, N.R., Falkenberg-Klok, J., Josephsen, J., (1996) Appl. Environ. Microbiol., vol. 62, pp. 3494-3498."
],
"prototype": "SfeI",
"organism": "Lactococcus lactis subsp. cremoris W56",
"methylation_site": ""
},
"bth1140i": {
"target_site": "GATC",
"name": "Bth1140I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. yunnanensis",
"methylation_site": ""
},
"ngoax": {
"target_site": "CCACC",
"name": "NgoAX",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Adamczyk-Poplawska, M., Lower, M., Piekarowicz, A., (2009) FEMS Microbiol. Lett., vol. 300, pp. 25-35.",
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Lewis, L.A. et al., Unpublished observations.",
"Monaci, E., Masignani, V., Pizza, M., Fontana, M.R., Japanese Patent Office, 2004.",
"Roe, B.A., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "3(6)"
},
"ngoav": {
"target_site": "GCANNNNNNNNTGC",
"name": "NgoAV",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Lewis, L.A. et al., Unpublished observations.",
"Piekarowicz, A., Klyz, A., Kwiatek, A., Stein, D.C., (2001) Mol. Microbiol., vol. 41, pp. 1199-1210."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "3(6)"
},
"sfai": {
"target_site": "GG^CC",
"name": "SfaI",
"suppliers": [],
"source": "R. Wu",
"references": [
"Wu, R., King, C.T., Jay, E., (1978) Gene, vol. 4, pp. 329-336."
],
"prototype": "HaeIII",
"organism": "Streptococcus faecalis var. zymogenes",
"methylation_site": ""
},
"bse118i": {
"target_site": "R^CCGGY",
"name": "Bse118I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Babkin, I.V., Tereshchenko, T.A., (1993) Bioorg. Khim., vol. 19, pp. 406-409."
],
"prototype": "Cfr10I",
"organism": "Bacillus species 118",
"methylation_site": ""
},
"vcho70i": {
"target_site": "TCGCGA",
"name": "VchO70I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "NruI",
"organism": "Vibrio cholerae 1111-77",
"methylation_site": ""
},
"eco99i": {
"target_site": "CCGCGG",
"name": "Eco99I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "SacII",
"organism": "Escherichia coli RFL99",
"methylation_site": ""
},
"pcii": {
"target_site": "A^CATGT",
"name": "PciI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 1781",
"references": [
"Abdurashitov, M.A., Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Degtyarev, S.K., Unpublished observations.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chan, S.-h., International Patent Office, 2009.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "BspLU11I",
"organism": "Planococcus citreus SE-F45",
"methylation_site": ""
},
"pspn4i": {
"target_site": "GGN^NCC",
"name": "PspN4I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Pseudomonas species N4",
"methylation_site": ""
},
"cfr14i": {
"target_site": "YGGCCR",
"name": "Cfr14I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "CfrI",
"organism": "Citrobacter freundii RFL14",
"methylation_site": ""
},
"csp5i": {
"target_site": "GATC",
"name": "Csp5I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Corynebacterium species RFL5",
"methylation_site": ""
},
"bsu1259i": {
"target_site": "?",
"name": "Bsu1259I",
"suppliers": [],
"source": "IAM 1259",
"references": [
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "",
"organism": "Bacillus subtilis",
"methylation_site": ""
},
"bco631i": {
"target_site": "GATNNNNATC",
"name": "Bco631I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Tereshchenko, T.A., Lebedev, L.R., Russian Patent Office, 1996."
],
"prototype": "BsaBI",
"organism": "Bacillus coagulans 631",
"methylation_site": ""
},
"csiai": {
"target_site": "A^CCGGT",
"name": "CsiAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., (1995) Vestn. Akad. Med. Nauk SSSR, vol. 2, pp. 47-51."
],
"prototype": "AgeI",
"organism": "Corynebacterium species A",
"methylation_site": ""
},
"eco70i": {
"target_site": "CCWGG",
"name": "Eco70I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL70",
"methylation_site": ""
},
"uba1248i": {
"target_site": "CTCGAG",
"name": "Uba1248I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1248",
"methylation_site": ""
},
"vchn100i": {
"target_site": "GAATTC",
"name": "VchN100I",
"suppliers": [],
"source": "C. Yamada",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Vibrio cholerae CY100",
"methylation_site": ""
},
"ecl1i": {
"target_site": "CCGCGG",
"name": "Ecl1I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Enterobacter cloacae RFL1",
"methylation_site": ""
},
"bsoai": {
"target_site": "GATATC",
"name": "BsoAI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Bacillus stearothermophilus JN1891",
"methylation_site": ""
},
"bsrci": {
"target_site": "ATCGAT",
"name": "BsrCI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus stearothermophilus C1825",
"methylation_site": ""
},
"pspdi": {
"target_site": "TCGCGA",
"name": "PspDI",
"suppliers": [],
"source": "NEB 1538",
"references": [
"Xu, D., Xu, S.-Y., Unpublished observations."
],
"prototype": "NruI",
"organism": "Pseudomonas species",
"methylation_site": ""
},
"llakr2i": {
"target_site": "GATC",
"name": "LlaKR2I",
"suppliers": [],
"source": "D.P. Twomey",
"references": [
"Twomey, D.P., McKay, L.L., O'Sullivan, D.J., (1997) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 97, pp. 426."
],
"prototype": "MboI",
"organism": "Lactococcus lactis subsp. lactis KR2",
"methylation_site": "4(5)"
},
"uba1136i": {
"target_site": "TCCGGA",
"name": "Uba1136I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Unidentified bacterium RFL1136",
"methylation_site": ""
},
"blohii": {
"target_site": "CTGCA^G",
"name": "BloHII",
"suppliers": [],
"source": "ATCC 15707",
"references": [
"Hartke, A., Benachour, A., Boutibonnes, P., Auffray, Y., (1996) Appl. Microbiol. Biotechnol., vol. 45, pp. 132-136."
],
"prototype": "PstI",
"organism": "Bifidobacterium longum E194b",
"methylation_site": ""
},
"ftnuiii": {
"target_site": "?",
"name": "FtnUIII",
"suppliers": [],
"source": "F. Nano",
"references": [
"Gallagher, L.A., McKevitt, M., Ramage, E.R., Manoil, C., (2008) J. Bacteriol., vol. 190, pp. 7830-7837."
],
"prototype": "",
"organism": "Francisella tularensis subsp. novicida U112",
"methylation_site": "?(6)"
},
"aflii": {
"target_site": "C^TTAAG",
"name": "AflII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)"
],
"source": "CCAP 1403/13f",
"references": [
"Lunnen, K.D., Wilson, G.G., US Patent Office, 1991.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Whitehead, P.R., Brown, N.L., (1985) J. Gen. Microbiol., vol. 131, pp. 951-958.",
"Wilson, G.G., Lunnen, K.D., Unpublished observations."
],
"prototype": "",
"organism": "Anabaena flos-aquae",
"methylation_site": ""
},
"fspbi": {
"target_site": "C^TAG",
"name": "FspBI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Starkovska, E., Maneliene, Z., Steponaviciene, D., Trinkunaite, L., Kiuduliene, L., Petrusyte, M., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Flavobacterium species RFLI",
"methylation_site": ""
},
"bsani": {
"target_site": "CCWGG",
"name": "BsaNI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus CPW1",
"methylation_site": ""
},
"trsti": {
"target_site": "GATC",
"name": "TrsTI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193.",
"Piknova, M., Pristas, P., Javorsky, P., Kasperowic, A., Michalowski, T., (2004) Lett. Appl. Microbiol., vol. 38, pp. 311-314."
],
"prototype": "MboI",
"organism": "Treponema species T",
"methylation_site": "2(6)"
},
"bstki": {
"target_site": "TGATCA",
"name": "BstKI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "BclI",
"organism": "Bacillus stearothermophilus K554",
"methylation_site": ""
},
"afliv": {
"target_site": "AGTACT",
"name": "AflIV",
"suppliers": [],
"source": "CCAP 1403/13f",
"references": [
"Groot, E., Schouten, J.P., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Anabaena flos-aquae",
"methylation_site": ""
},
"sflhk2374i": {
"target_site": "CCWGG",
"name": "SflHK2374I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 2374",
"methylation_site": ""
},
"sag16i": {
"target_site": "CTGCA^G",
"name": "Sag16I",
"suppliers": [],
"source": "I. Mikula",
"references": [
"Godany, A., Bukovska, G., Farkasovska, J., Brnakova, Z., Dmitriev, A., Tkacikova, L., Ayele, T., Mikula, I., (2004) Folia Microbiol. (Praha), vol. 49, pp. 307-314."
],
"prototype": "PstI",
"organism": "Streptococcus agalactiae 16",
"methylation_site": ""
},
"hins1i": {
"target_site": "GCGC",
"name": "HinS1I",
"suppliers": [],
"source": "S. Shen",
"references": [
"Shen, S., Li, Q., Yan, P., Zhou, B., Ye, S., Lu, Y., Wang, D., (1980) Sci. Sin., vol. 23, pp. 1435-1442."
],
"prototype": "HhaI",
"organism": "Haemophilus influenzae S1",
"methylation_site": ""
},
"rsri": {
"target_site": "G^AATTC",
"name": "RsrI",
"suppliers": [],
"source": "S. Kaplan",
"references": [
"Aiken, C., Gumport, R.I., (1988) Nucleic Acids Res., vol. 16, pp. 7901-7916.",
"Aiken, C., Milarski-Brown, K., Gumport, R.I., (1986) Fed. Proc., vol. 45, pp. 1914.",
"Gardner, J.F., Cohen, L.K., Lynn, S.P., Kaplan, S., Unpublished observations.",
"Greene, P.J., Ballard, B.T., Stephenson, F., Kohr, W.J., Rodriguez, H., Rosenberg, J.M., Boyer, H.W., (1988) Gene, vol. 68, pp. 43-52.",
"Kaszubska, W., Aiken, C., O'Connor, C.D., Gumport, R.I., (1989) Nucleic Acids Res., vol. 17, pp. 10403-10425."
],
"prototype": "EcoRI",
"organism": "Rhodopseudomonas sphaeroides",
"methylation_site": "3(6)"
},
"nspai": {
"target_site": "GATC",
"name": "NspAI",
"suppliers": [],
"source": "ATCC 19170",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "MboI",
"organism": "Nocardia species",
"methylation_site": ""
},
"eco127i": {
"target_site": "GGTCTC",
"name": "Eco127I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL127",
"methylation_site": ""
},
"bpa34i": {
"target_site": "AGT^ACT",
"name": "Bpa34I",
"suppliers": [],
"source": "NEB 1562",
"references": [
"Pan, X.S., Morgan, R., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Bacillus pallidus",
"methylation_site": ""
},
"sflhk10790i": {
"target_site": "CCWGG",
"name": "SflHK10790I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 10790",
"methylation_site": ""
},
"bspovii": {
"target_site": "AT^CGAT",
"name": "BspOVII",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Chernov, A.V., Lepikhov, K.A., Zheleznaya, L.A., Matvienko, N.I., (1996) Biokhimiia, vol. 61, pp. 1837-1847."
],
"prototype": "ClaI",
"organism": "Bacillus species OV",
"methylation_site": ""
},
"tfitok4a2i": {
"target_site": "TCGA",
"name": "TfiTok4A2I",
"suppliers": [],
"source": "F. Barany",
"references": [
"Cao, W., Lu, J., Barany, F., (1997) Gene, vol. 197, pp. 205-214."
],
"prototype": "TaqI",
"organism": "Thermus filiformis Tok4A2",
"methylation_site": ""
},
"bsp90ii": {
"target_site": "GGATCC",
"name": "Bsp90II",
"suppliers": [],
"source": "ITI 90",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Bacillus species",
"methylation_site": ""
},
"eco50i": {
"target_site": "GGYRCC",
"name": "Eco50I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Escherichia coli RFL50",
"methylation_site": ""
},
"hin7i": {
"target_site": "GCGC",
"name": "Hin7I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "HhaI",
"organism": "Haemophilus influenzae RFL7",
"methylation_site": ""
},
"vcho87i": {
"target_site": "CTGCAG",
"name": "VchO87I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "PstI",
"organism": "Vibrio cholerae 973-81",
"methylation_site": ""
},
"uba1453i": {
"target_site": "ATCGAT",
"name": "Uba1453I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1453",
"methylation_site": ""
},
"eco47iii": {
"target_site": "AGC^GCT",
"name": "Eco47III",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Roche Applied Science (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Butkus, V., (1983) FEBS Lett., vol. 161, pp. 213-216."
],
"prototype": "",
"organism": "Escherichia coli RFL47",
"methylation_site": ""
},
"uba1156i": {
"target_site": "GGGCCC",
"name": "Uba1156I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ApaI",
"organism": "Unidentified bacterium RFL1156",
"methylation_site": ""
},
"bsrbi": {
"target_site": "CCGCTC(-3/-3)",
"name": "BsrBI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations.",
"Heiter, D., Nwankwo, D.O., Wilson, G.G., Unpublished observations.",
"Heiter, D.F., Nwankwo, D.O., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus CPW193",
"methylation_site": "?(4)"
},
"paeqi": {
"target_site": "CCGC^GG",
"name": "PaeQI",
"suppliers": [],
"source": "CAMB 2637",
"references": [
"Choudhry, S., (1999) Proc. Pakistan Acad. Sci., vol. 36, pp. 165-171."
],
"prototype": "SacII",
"organism": "Pseudomonas aeruginosa Q2",
"methylation_site": ""
},
"cvibi": {
"target_site": "G^ANTC",
"name": "CviBI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Kan, T.N., Lin, L., Chandrasegaran, S., (1992) Gene, vol. 121, pp. 1-7.",
"Xia, Y., Burbank, D.E., Van Etten, J.L., (1986) Nucleic Acids Res., vol. 14, pp. 6017-6030."
],
"prototype": "HinfI",
"organism": "Chlorella strain NC64A (NC-1A)",
"methylation_site": "2(6)"
},
"bbri": {
"target_site": "A^AGCTT",
"name": "BbrI",
"suppliers": [],
"source": "T.I. Tikchonenko",
"references": [
"Kalinin, V.N., Lapaeva, I.A., Lunin, V.G., Skripkin, E.A., Smirnov, V.D., Tikchonenko, T.I., (1986) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 16-19."
],
"prototype": "HindIII",
"organism": "Bordetella bronchiseptica 4994",
"methylation_site": ""
},
"bstz7i": {
"target_site": "GRGCYC",
"name": "BstZ7I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Bacillus stearothermophilus 26W",
"methylation_site": ""
},
"ecor12i": {
"target_site": "?",
"name": "EcoR12I",
"suppliers": [],
"source": "ATCC 35331",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR12",
"methylation_site": "?(6)"
},
"bstsfi": {
"target_site": "C^TRYAG",
"name": "BstSFI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Bacillus stearothermophilus SF",
"methylation_site": ""
},
"bspf4i": {
"target_site": "G^GNCC",
"name": "BspF4I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Zharmukhamedova, T.Y., Privezencova, N.G., Shishova, O.V., Shiryaev, S.A., Zheleznaya, L.A., Matvienko, N.I., (1999) Biokhimiia, vol. 64, pp. 697-702."
],
"prototype": "AsuI",
"organism": "Bacillus species F4",
"methylation_site": ""
},
"vha44i": {
"target_site": "GATC",
"name": "Vha44I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "MboI",
"organism": "Vibrio harveyi 44",
"methylation_site": ""
},
"bcibii": {
"target_site": "CC^WGG",
"name": "BciBII",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Eldarov, M.A., Karpichev, I.V., Samko, O.T., Anikeitcheva, N.V., Kalugin, A.A., Khoroshoutina, E.B., Sokolov, N.N., (1992) Nucleic Acids Res., vol. 20, pp. 2896."
],
"prototype": "EcoRII",
"organism": "Bacillus circulans B",
"methylation_site": ""
},
"hpyf71i": {
"target_site": "TCGA",
"name": "HpyF71I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL71",
"methylation_site": ""
},
"hpyf24i": {
"target_site": "TCGA",
"name": "HpyF24I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL24",
"methylation_site": ""
},
"r1.ssu4109i": {
"target_site": "GATC",
"name": "R1.Ssu4109I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis strain 89-4109-1",
"methylation_site": ""
},
"bstdz247i": {
"target_site": "CCCGT",
"name": "BstDZ247I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "BscGI",
"organism": "Bacillus stearothermophilus DZ247",
"methylation_site": ""
},
"alubi": {
"target_site": "AG^CT",
"name": "AluBI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Boltengagen, A.A., Tarasova, G.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations.",
"Chernukhin, V.A., Boltengagen, A.A., Tarasova, G.V., Dedkov, V.S., Degtyarev, S.K., (2007) Ovchinnikov Bull. Biotechnol. Phys. Chem. Biol., vol. 3, pp. 21-27."
],
"prototype": "AluI",
"organism": "Arthrobacter luteus B",
"methylation_site": ""
},
"lsp1109i": {
"target_site": "GCAGC(8/12)",
"name": "Lsp1109I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Jakubauskas, A., Kriukiene, E., Trinkunaite, L., Sapranauskas, R., Jurenaite-Urbanaviciene, S., Lubys, A., (2009) Plasmid, vol. 61, pp. 52-64.",
"Janulaitis, A., Grigaite, R., Trinkunaite, L., Maneliene, Z., Bitinaite, J., Kiuduliene, L., Butkus, V., Unpublished observations.",
"Lubys, A., Janulaitis, A., Unpublished observations.",
"Zakareviciene, L., Lubys, A., Unpublished observations."
],
"prototype": "BbvI",
"organism": "Listeria species RFL1109",
"methylation_site": "?(5)"
},
"pfl1108ii": {
"target_site": "CCGCGG",
"name": "Pfl1108II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Pseudomonas fluorescens RFL1108",
"methylation_site": ""
},
"pflbi": {
"target_site": "CCANNNN^NTGG",
"name": "PflBI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Waaijer, R.J.A., Unpublished observations."
],
"prototype": "PflMI",
"organism": "Pseudomonas fluorescens type B",
"methylation_site": ""
},
"hpyf48i": {
"target_site": "GTSAC",
"name": "HpyF48I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL48",
"methylation_site": ""
},
"bavbii": {
"target_site": "G^GNCC",
"name": "BavBII",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Anikeitcheva, N.V., Fitsner, A.B., Samko, O.T., Choroshoutina, E.B., Kalugin, A.A., Lidrik, G.I., Soviet Patent Office, 1992."
],
"prototype": "AsuI",
"organism": "Bacillus alvei B",
"methylation_site": ""
},
"bstlvi": {
"target_site": "ATCGAT",
"name": "BstLVI",
"suppliers": [],
"source": "C. Vasquez",
"references": [
"Lobos, C., Vasquez, C., (1993) Biochim. Biophys. Acta, vol. 1171, pp. 295-298."
],
"prototype": "ClaI",
"organism": "Bacillus stearothermophilus LV",
"methylation_site": ""
},
"fnuci": {
"target_site": "^GATC",
"name": "FnuCI",
"suppliers": [],
"source": "M. Smith",
"references": [
"Lui, A.C.P., McBride, B.C., Vovis, G.F., Smith, M., (1979) Nucleic Acids Res., vol. 6, pp. 1-15."
],
"prototype": "MboI",
"organism": "Fusobacterium nucleatum C",
"methylation_site": ""
},
"bsski": {
"target_site": "^CCNGG",
"name": "BssKI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 547",
"references": [
"Kong, H., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Bacillus stearothermophilus",
"methylation_site": "?(5)"
},
"hgifi": {
"target_site": "?",
"name": "HgiFI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Herpetosiphon giganteus Hpg14",
"methylation_site": ""
},
"m.dreldnmt3": {
"target_site": "?",
"name": "M.DreLDnmt3",
"suppliers": [],
"source": "E. Li",
"references": [
"Mhanni, A.A., Yoder, J.A., Dubesky, C., McGowan, R.A., (2001) Genesis, vol. 30, pp. 213-219.",
"Shimoda, N., Unpublished observations.",
"Shimoda, N., Yamakoshi, K., Miyake, A., Takeda, H., (2005) Dev. Dyn., vol. 233, pp. 1509-1516.",
"Xie, S., Wang, Z., Okano, M., Nogami, M., Li, Y., He, W.-W., Okumura, K., Li, E., (1999) Gene, vol. 236, pp. 87-95."
],
"prototype": "",
"organism": "Danio rerio",
"methylation_site": "?(5)"
},
"xmaii": {
"target_site": "CTGCAG",
"name": "XmaII",
"suppliers": [],
"source": "ATCC 9924",
"references": [
"Endow, S.A., Roberts, R.J., (1977) J. Mol. Biol., vol. 112, pp. 521-529."
],
"prototype": "PstI",
"organism": "Xanthomonas malvacearum",
"methylation_site": ""
},
"bsp76i": {
"target_site": "GATC",
"name": "Bsp76I",
"suppliers": [],
"source": "S.Y. Ye",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus 76",
"methylation_site": ""
},
"m.cvibii": {
"target_site": "GATC",
"name": "M.CviBII",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Narva, K.E., Wendell, D.L., Skrdla, M.P., Van Etten, J.L., (1987) Nucleic Acids Res., vol. 15, pp. 9807-9823.",
"Van Etten, J.L., Xia, Y., Burbank, D.E., (1989) J. Cell Biochem. Suppl., vol. 13D, pp. 199."
],
"prototype": "MboI",
"organism": "Chlorella strain NC64A (NC-1A)",
"methylation_site": ""
},
"apri": {
"target_site": "GCCGGC",
"name": "AprI",
"suppliers": [],
"source": "ATCC 31281",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Actinosynnema pretiosum",
"methylation_site": ""
},
"bse631i": {
"target_site": "GATNNNNATC",
"name": "Bse631I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D., Tereschenko, T., Chizikov, V.E., Andreeva, I., (1995) Gene, vol. 157, pp. 321-322."
],
"prototype": "BsaBI",
"organism": "Bacillus species 631",
"methylation_site": ""
},
"bim19i": {
"target_site": "TT^CGAA",
"name": "Bim19I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Prichodko, G.G., Degtyarev, S.K., Rechkunova, N.I., Sosnovsev, S.V., Tchigikov, V.E., (1990) Biotekhnologiya, vol. 1, pp. 12-16."
],
"prototype": "AsuII",
"organism": "Brevibacterium immotum 19",
"methylation_site": ""
},
"nladii": {
"target_site": "GGNCC",
"name": "NlaDII",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Stein, D.C., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Neisseria lactamica 5841",
"methylation_site": ""
},
"bsmi": {
"target_site": "GAATGC(1/-1)",
"name": "BsmI",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Sigma Chemical Corporation (1/11)"
],
"source": "N. Welker",
"references": [
"Christ, C., Ingalls, D., Unpublished observations.",
"Myers, P.A., Roberts, R.J., Unpublished observations.",
"Zhou, J., Zhu, Z., Xu, S.Y., US Patent Office, 2002."
],
"prototype": "",
"organism": "Bacillus stearothermophilus NUB36",
"methylation_site": ""
},
"bcibi": {
"target_site": "AT^CGAT",
"name": "BciBI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Samko, O.T., Kalugin, A.A., Eldarov, M.A., Karpychev, I.V., Anikeitcheva, N.V., Khoroshutina, E.B., Skryabin, K.G., Librik, G.I., Sokolov, N.N., (1994) Bioorg. Khim., vol. 20, pp. 1327-1333."
],
"prototype": "ClaI",
"organism": "Bacillus circulans B",
"methylation_site": ""
},
"scini": {
"target_site": "G^CGC",
"name": "SciNI",
"suppliers": [],
"source": "M.A. Stephens",
"references": [
"Stephens, M.A., (1982) J. Bacteriol., vol. 149, pp. 508-514."
],
"prototype": "HhaI",
"organism": "Spiroplasma citri ASP2",
"methylation_site": ""
},
"hpy178vi": {
"target_site": "GGATG",
"name": "Hpy178VI",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Blaser, M., Unpublished observations."
],
"prototype": "FokI",
"organism": "Helicobacter pylori J178",
"methylation_site": ""
},
"cviai": {
"target_site": "^GATC",
"name": "CviAI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Xia, Y., Burbank, D.E., Uher, L., Rabussay, D., Van Etten, J.L., (1986) Mol. Cell. Biol., vol. 6, pp. 1430-1439.",
"Xia, Y., Van Etten, J.L., (1986) Mol. Cell. Biol., vol. 6, pp. 1440-1445."
],
"prototype": "MboI",
"organism": "Chlorella strain NC64A (PBCV-1)",
"methylation_site": "2(6)"
},
"bsosi": {
"target_site": "AGTACT",
"name": "BsoSI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Bacillus stearothermophilus SR65",
"methylation_site": ""
},
"rhci": {
"target_site": "TCATGA",
"name": "RhcI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "BspHI",
"organism": "Rhodococcus species SE1991",
"methylation_site": ""
},
"uba88i": {
"target_site": "GGATCC",
"name": "Uba88I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL88",
"methylation_site": ""
},
"vpak57i": {
"target_site": "GGTCTC",
"name": "VpaK57I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Kotani, H., Mise, K., (1992) Gene, vol. 117, pp. 103-106."
],
"prototype": "Eco31I",
"organism": "Vibrio parahaemolyticus T4144-1",
"methylation_site": ""
},
"bspui": {
"target_site": "GCSG^C",
"name": "BspUI",
"suppliers": [],
"source": "NEB 1593",
"references": [
"Abila, P., Nanyonjo, H., Egwang, T.G., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "TauI",
"organism": "Bacillus species",
"methylation_site": ""
},
"bspts514i": {
"target_site": "GAAGAC(2/6)",
"name": "BspTS514I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kovalevskaya, N.P., Zelinskaya, N.V., Zheleznaya, L.A., Matvienko, N.I., (1993) Bioorg. Khim., vol. 19, pp. 1073-1076."
],
"prototype": "BbvII",
"organism": "Bacillus species TS514",
"methylation_site": ""
},
"mph1103ii": {
"target_site": "GATC",
"name": "Mph1103II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Maneliene, Z., Capskaya, L., Kiuduliene, L., Butkus, V., Unpublished observations."
],
"prototype": "DpnI",
"organism": "Moraxella phenylpyruvica RFL1103",
"methylation_site": ""
},
"bsex3i": {
"target_site": "C^GGCCG",
"name": "BseX3I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Kileva, E.V., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "XmaIII",
"organism": "Bacillus stearothermophilus X3",
"methylation_site": ""
},
"sflhk11572i": {
"target_site": "CCSGG",
"name": "SflHK11572I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "CauII",
"organism": "Shigella flexneri 11572",
"methylation_site": ""
},
"acaiii": {
"target_site": "TGCGCA",
"name": "AcaIII",
"suppliers": [],
"source": "CCAP 1403/1",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "MstI",
"organism": "Anabaena catenula",
"methylation_site": ""
},
"hins2i": {
"target_site": "GCGC",
"name": "HinS2I",
"suppliers": [],
"source": "S. Shen",
"references": [
"Shen, S., Li, Q., Yan, P., Zhou, B., Ye, S., Lu, Y., Wang, D., (1980) Sci. Sin., vol. 23, pp. 1435-1442."
],
"prototype": "HhaI",
"organism": "Haemophilus influenzae S2",
"methylation_site": ""
},
"mcaci": {
"target_site": "CCATC",
"name": "McaCI",
"suppliers": [],
"source": "ATCC 27343",
"references": [
"Glass, J.I., Lartigue, C., Pfannkoch, C., Baden-Tillson, H., Smith, H.O., Venter, J.C., Roske, K., Wise, K.S., Calcutt, M.J., Nelson, W.C., Nierman, W.C., Unpublished observations.",
"Lartigue, C., Vashee, S., Algire, M.A., Chuang, R.Y., Benders, G.A., Ma, L., Noskov, V.N., Denisova, E.A., Gibson, D.G., Assad-Garcia, N., Alperovich, N., Thomas, D.W., Merryman, C., Hutchison, C.A.I.I.I., Smith, H.O., Venter, J.C., Glass, J.I., (2009) Science, vol. 325, pp. 1693-1696."
],
"prototype": "BccI",
"organism": "Mycoplasma capricolum subsp. capricolum ATCC 27343",
"methylation_site": ""
},
"bpuhi": {
"target_site": "TTCGAA",
"name": "BpuHI",
"suppliers": [],
"source": "NEB 1360",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus pumilus 1520",
"methylation_site": ""
},
"m.bstnbii": {
"target_site": "GASTC",
"name": "M.BstNBII",
"suppliers": [],
"source": "NEB 928",
"references": [
"Higgins, L.S., Holden, M., Kong, H., Unpublished observations.",
"Kong, H., Higgins, L.S., US Patent Office, 2002."
],
"prototype": "PleI",
"organism": "Bacillus stearothermophilus",
"methylation_site": ""
},
"mvaai": {
"target_site": "CGCG",
"name": "MvaAI",
"suppliers": [],
"source": "J.N. Reeve",
"references": [
"Morgan, R., Reeve, J., Krzycki, J., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Methanobacterium vannuli",
"methylation_site": ""
},
"tru28i": {
"target_site": "GGWCC",
"name": "Tru28I",
"suppliers": [],
"source": "ITI 28",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Thermus ruber",
"methylation_site": ""
},
"shyi": {
"target_site": "CCGCGG",
"name": "ShyI",
"suppliers": [],
"source": "F. Walter",
"references": [
"Walter, F., Hartmann, M., Roth, M., (1978) Abstracts of 12th FEBS Symposium, Dresden., vol. 0, pp. 648."
],
"prototype": "SacII",
"organism": "Streptomyces hygroscopicus 0477",
"methylation_site": ""
},
"schzi": {
"target_site": "CCGC^GG",
"name": "SchZI",
"suppliers": [],
"source": "M.-C. Kao",
"references": [
"Hu, K.-Y., Wuu, J.-A., Kao, M.-C., Liu, Y.-T., Pai, S.-H., (1998) Appl. Biochem. Biotechnol., vol. 73, pp. 231-241."
],
"prototype": "SacII",
"organism": "Streptomyces chusanensis ZS-2",
"methylation_site": ""
},
"uba1266i": {
"target_site": "CTTAAG",
"name": "Uba1266I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AflII",
"organism": "Unidentified bacterium RFL1266",
"methylation_site": ""
},
"ecot95i": {
"target_site": "GRGCYC",
"name": "EcoT95I",
"suppliers": [],
"source": "N. Terakado",
"references": [
"Mise, K., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli TH95",
"methylation_site": ""
},
"hpy26ii": {
"target_site": "TCGA",
"name": "Hpy26II",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Ando, T., Xu, Q., Torres, M., Kusugami, K., Israel, D.A., Blaser, M.J., (2000) Mol. Microbiol., vol. 37, pp. 1052-1065."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori J26",
"methylation_site": ""
},
"avri": {
"target_site": "CYCGRG",
"name": "AvrI",
"suppliers": [],
"source": "E.C. Rosenvold",
"references": [
"Rosenvold, E.C., Szybalski, W., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Anabaena variabilis uw",
"methylation_site": ""
},
"bsph22i": {
"target_site": "TTCGAA",
"name": "BspH22I",
"suppliers": [],
"source": "ITI 22",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"eco32i": {
"target_site": "GAT^ATC",
"name": "Eco32I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129.",
"Maneliene, Z., Bitinaite, J., Butkus, V., Janulaitis, A., Unpublished observations.",
"Vaisvila, R., Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Escherichia coli RFL32",
"methylation_site": ""
},
"spodi": {
"target_site": "GCGGRAG",
"name": "SpoDI",
"suppliers": [],
"source": "M.A. Moran",
"references": [
"Moran, M.A. et al., (2004) Nature, vol. 432, pp. 910-913.",
"Morgan, R.D., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Silicibacter pomeroyi DSS-3",
"methylation_site": "?(6)"
},
"hpyf70i": {
"target_site": "CTNAG",
"name": "HpyF70I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL70",
"methylation_site": ""
},
"sth302ii": {
"target_site": "CC^GG",
"name": "Sth302II",
"suppliers": [],
"source": "G. Guedon",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Streptococcus thermophilus CNRZ302",
"methylation_site": ""
},
"bamnxi": {
"target_site": "G^GWCC",
"name": "BamNxI",
"suppliers": [],
"source": "T. Ando",
"references": [
"Ikawa, S., Shibata, T., Ando, T., (1979) Agric. Biol. Chem., vol. 43, pp. 873-875.",
"Shibata, T., Ando, T., (1975) Mol. Gen. Genet., vol. 138, pp. 269-279."
],
"prototype": "AvaII",
"organism": "Bacillus amyloliquefaciens N",
"methylation_site": ""
},
"bfu1570i": {
"target_site": "GWGCWC",
"name": "Bfu1570I",
"suppliers": [],
"source": "NEB 1301",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Bacillus fusiformis 1570",
"methylation_site": ""
},
"eco225i": {
"target_site": "GGTCTC",
"name": "Eco225I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL225",
"methylation_site": ""
},
"bstfz438i": {
"target_site": "CCCGC(4/6)",
"name": "BstFZ438I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "FauI",
"organism": "Bacillus stearothermophilus FZ438",
"methylation_site": ""
},
"bspis4i": {
"target_site": "GAAGAC(2/6)",
"name": "BspIS4I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kovalevskaya, N.P., Zelinskaya, N.V., Zheleznaya, L.A., Matvienko, N.I., (1993) Bioorg. Khim., vol. 19, pp. 1073-1076.",
"Zelinskaya, N.V., Kovalevskaya, N.P., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (1995) Biokhimiia, vol. 60, pp. 1435-1448."
],
"prototype": "BbvII",
"organism": "Bacillus species IS4",
"methylation_site": "?(6)"
},
"hpyf49v": {
"target_site": "TGCA",
"name": "HpyF49V",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori RFL49",
"methylation_site": ""
},
"pph1773i": {
"target_site": "GGNCC",
"name": "Pph1773I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Photobacterium phosphoreum 1773",
"methylation_site": ""
},
"cvili": {
"target_site": "RGCY",
"name": "CviLI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Agarwal, K., Unpublished observations."
],
"prototype": "CviJI",
"organism": "Chlorella strain NC64A (CA-2A)",
"methylation_site": ""
},
"uba1196i": {
"target_site": "ATCGAT",
"name": "Uba1196I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1196",
"methylation_site": ""
},
"hindii": {
"target_site": "GTY^RAC",
"name": "HindII",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Roche Applied Science (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "ATCC 51907",
"references": [
"Fleischmann, R.D. et al., (1995) Science, vol. 269, pp. 496-512.",
"Fleischmann, R.D., Adams, M.D., White, O., Smith, H.O., Venter, J.C., Japanese Patent Office, 2004.",
"Fleischmann, R.D., Adams, M.D., White, O., Smith, H.O., Venter, J.C., US Patent Office, 2005.",
"Kelly, T.J. Jr., Smith, H.O., (1970) J. Mol. Biol., vol. 51, pp. 393-409.",
"Roy, P.H., Smith, H.O., (1973) J. Mol. Biol., vol. 81, pp. 445-459.",
"Roy, P.H., Smith, H.O., (1973) J. Mol. Biol., vol. 81, pp. 427-444.",
"Smith, H.O., Wilcox, K.W., (1970) J. Mol. Biol., vol. 51, pp. 379-391."
],
"prototype": "",
"organism": "Haemophilus influenzae Rd",
"methylation_site": "5(6)"
},
"sagi": {
"target_site": "GGCC",
"name": "SagI",
"suppliers": [],
"source": "M.T. Poch",
"references": [
"Poch, M.T., Somkuti, G.A., (1994) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 94, pp. 385.",
"Poch, M.T., Somkuti, G.A., (1995) Appl. Microbiol. Biotechnol., vol. 43, pp. 282-284."
],
"prototype": "HaeIII",
"organism": "Streptococcus agalactiae B96",
"methylation_site": ""
},
"ntasii": {
"target_site": "GCCGGC",
"name": "NtaSII",
"suppliers": [],
"source": "ATCC 31190",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Nocardia tartaricans",
"methylation_site": ""
},
"cfr33i": {
"target_site": "GGNCC",
"name": "Cfr33I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL33",
"methylation_site": ""
},
"ngocii": {
"target_site": "GGCC",
"name": "NgoCII",
"suppliers": [],
"source": "CDC 66",
"references": [
"Clanton, D.J., Woodward, J.M., Miller, R.V., (1978) J. Bacteriol., vol. 135, pp. 270-273."
],
"prototype": "HaeIII",
"organism": "Neisseria gonorrhoeae",
"methylation_site": ""
},
"vpak7ai": {
"target_site": "GGWCC",
"name": "VpaK7AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus TVP1740",
"methylation_site": ""
},
"bsu22i": {
"target_site": "TCCGGA",
"name": "Bsu22I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kramarov, V.M., Skrypina, N.A., Smolyaninov, V.V., Smirnov, V.V., Resnik, S.R., Sorokulova, I.B., Matvienko, N.I., (1989) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 42-45."
],
"prototype": "BspMII",
"organism": "Bacillus subtilis 22",
"methylation_site": ""
},
"sol10179i": {
"target_site": "C^TCGAG",
"name": "Sol10179I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces olivochromogenes ST10179",
"methylation_site": ""
},
"bsp61i": {
"target_site": "GATC",
"name": "Bsp61I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL61",
"methylation_site": ""
},
"ppui": {
"target_site": "GGCC",
"name": "PpuI",
"suppliers": [],
"source": "Toyobo",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "HaeIII",
"organism": "Pseudomonas putida C-83",
"methylation_site": ""
},
"ccop31i": {
"target_site": "GATC",
"name": "CcoP31I",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vitor, J.M.B., Polisson, C., Cabrita, J., Silva, R.V.C., (1993) Acta Gastroenterol. Belg., vol. 56, pp. 41."
],
"prototype": "MboI",
"organism": "Campylobacter coli P31",
"methylation_site": ""
},
"pmdi": {
"target_site": "CCTCAGC",
"name": "PmdI",
"suppliers": [],
"source": "Fermentas G350",
"references": [
"Vitkute, J., Valiulyte, D., Trinkunaite, L., Norgeliene, D., Janulaitis, A., Unpublished observations."
],
"prototype": "BbvCI",
"organism": "Pseudomonas mandelii RFL49",
"methylation_site": ""
},
"m.aimaii": {
"target_site": "?",
"name": "M.AimAII",
"suppliers": [],
"source": "J. Walter",
"references": [
"Chernov, A.V., Vollmayr, P., Walter, J., Trautner, T.A., (1997) Biol. Chem., vol. 378, pp. 1467-1473."
],
"prototype": "",
"organism": "Ascobolus immersus",
"methylation_site": "?(5)"
},
"mspci": {
"target_site": "C^TTAAG",
"name": "MspCI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "V. Bouriotis",
"references": [
"Rina, M., Tzanodaskalaki, M., Karagouni, A., Pagomenou, M., Bouriotis, V., (1992) Nucleic Acids Res., vol. 20, pp. 1806."
],
"prototype": "AflII",
"organism": "Micrococcus species",
"methylation_site": ""
},
"pfl16i": {
"target_site": "GATATC",
"name": "Pfl16I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Pseudomonas fluoroviolaceus RFL16",
"methylation_site": ""
},
"hpy501i": {
"target_site": "GTAC",
"name": "Hpy501I",
"suppliers": [],
"source": "N.R. Salama",
"references": [
"Humbert, O., Salama, N.R., (2008) Nucleic Acids Res., vol. 36, pp. 6893-6906.",
"Salama, N.R., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori 50-1",
"methylation_site": ""
},
"pae17i": {
"target_site": "CCGCGG",
"name": "Pae17I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Pseudomonas aeruginosa RFL17",
"methylation_site": ""
},
"bsp141i": {
"target_site": "CTCGAG",
"name": "Bsp141I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus species RFL141",
"methylation_site": ""
},
"pstnhi": {
"target_site": "G^CTAGC",
"name": "PstNHI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Myakisheva, T.V., Dedkov, V.S., Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NheI",
"organism": "Pseudomonas stutzeri NH",
"methylation_site": ""
},
"cfr52i": {
"target_site": "GGNCC",
"name": "Cfr52I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL52",
"methylation_site": ""
},
"apaci": {
"target_site": "G^GATCC",
"name": "ApaCI",
"suppliers": [],
"source": "J. Grones",
"references": [
"Grones, J., Turna, J., (1992) Nucleic Acids Res., vol. 20, pp. 3513."
],
"prototype": "BamHI",
"organism": "Acetobacter pasteurianus C",
"methylation_site": ""
},
"bst29i": {
"target_site": "CCTNAGG",
"name": "Bst29I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Serov, G.D., Tereschenko, T.A., Puchkova, L.I., Repin, V.E., Unpublished observations."
],
"prototype": "SauI",
"organism": "Bacillus stearothermophilus 29",
"methylation_site": ""
},
"rflfii": {
"target_site": "AGT^ACT",
"name": "RflFII",
"suppliers": [],
"source": "B.A. White",
"references": [
"Morrison, M., Mackie, R.I., White, B.A., (1992) Aust. Microbiol., vol. 0, pp. 301-303.",
"Morrison, M., Mackie, R.I., White, B.A., (1994) FEMS Microbiol. Lett., vol. 122, pp. 181-185."
],
"prototype": "ScaI",
"organism": "Ruminococcus flavefaciens FD-1",
"methylation_site": ""
},
"bspj67i": {
"target_site": "CCSGG",
"name": "BspJ67I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "CauII",
"organism": "Bacillus species RFL67",
"methylation_site": ""
},
"bstnz169i": {
"target_site": "AT^CGAT",
"name": "BstNZ169I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus stearothermophilus NZ169",
"methylation_site": ""
},
"ptei": {
"target_site": "G^CGCGC",
"name": "PteI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G357",
"references": [
"Vitkute, J., Lauciuniene, N., Lapcinskaja, S., Trinkunaite, L., Zakareviciene, L., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Paenibacillus terrae RFL2",
"methylation_site": ""
},
"pae177i": {
"target_site": "GGATCC",
"name": "Pae177I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Pseudomonas aeruginosa RFL177",
"methylation_site": ""
},
"bsiri": {
"target_site": "?",
"name": "BsiRI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"vpak4ai": {
"target_site": "CTGCAG",
"name": "VpaK4AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "PstI",
"organism": "Vibrio parahaemolyticus TVP1622",
"methylation_site": ""
},
"bseji": {
"target_site": "GATNN^NNATC",
"name": "BseJI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Lazareviciute, L., Maneliene, Z., Capskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BsaBI",
"organism": "Bacillus stearothermophilus Tsp5",
"methylation_site": ""
},
"uba1211i": {
"target_site": "CTGCAG",
"name": "Uba1211I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1211",
"methylation_site": ""
},
"lsp1270i": {
"target_site": "RCATGY",
"name": "Lsp1270I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Krikstulyte, V., Trinkunaite, L., Maneliene, Z., Kiuduliene, L., Petrusyte, M., Butkus, V., Unpublished observations."
],
"prototype": "NspI",
"organism": "Listeria species RFL1270",
"methylation_site": ""
},
"acc36i": {
"target_site": "ACCTGC(4/8)",
"name": "Acc36I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BspMI",
"organism": "Acinetobacter calcoaceticus 36",
"methylation_site": ""
},
"pae14i": {
"target_site": "CTGCAG",
"name": "Pae14I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL14",
"methylation_site": ""
},
"esp15i": {
"target_site": "GGYRCC",
"name": "Esp15I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL15",
"methylation_site": ""
},
"svoi": {
"target_site": "CRCCGGYG",
"name": "SvoI",
"suppliers": [],
"source": "Fermentas G353",
"references": [
"Vitkute, J., Valiulyte, D., Norgeliene, D., Trinkunaite, L., Janulaitis, A., Unpublished observations."
],
"prototype": "SgrAI",
"organism": "Streptomyces violaceoruber RFL10",
"methylation_site": ""
},
"hpyjp26ii": {
"target_site": "TCGA",
"name": "HpyJP26II",
"suppliers": [],
"source": "T. Ando",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori JP26",
"methylation_site": ""
},
"pph2059i": {
"target_site": "CTGCAG",
"name": "Pph2059I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "PstI",
"organism": "Photobacterium phosphoreum 2059",
"methylation_site": ""
},
"bla7920i": {
"target_site": "TCCGGA",
"name": "Bla7920I",
"suppliers": [],
"source": "G.N. Gabrichevsky",
"references": [
"Skrypina, N.A., Kramarov, V.M., Lyannaya, A.M., Smolyaninov, V.V., Goncharova, G.I., (1988) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 15-16."
],
"prototype": "BspMII",
"organism": "Bifidobacterium lactentis 7920",
"methylation_site": ""
},
"asuc2i": {
"target_site": "CC^SGG",
"name": "AsuC2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Kileva, E.V., Dedkov, V.S., Abdurashitov, M.A., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "CauII",
"organism": "Actinobacillus suis C2",
"methylation_site": ""
},
"bsp9i": {
"target_site": "GATC",
"name": "Bsp9I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL9",
"methylation_site": ""
},
"ecor24i": {
"target_site": "?",
"name": "EcoR24I",
"suppliers": [],
"source": "ATCC 35343",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR24",
"methylation_site": "?(6)"
},
"uba46i": {
"target_site": "CTGCAG",
"name": "Uba46I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL46",
"methylation_site": ""
},
"aani": {
"target_site": "TTA^TAA",
"name": "AanI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G324",
"references": [
"Vitkute, J., Lapcinskaja, S., Capskaja, L., Zakareviciene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "PsiI",
"organism": "Arthrobacter aurescens RFL2",
"methylation_site": ""
},
"smui": {
"target_site": "CCCGC(4/6)",
"name": "SmuI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Grigaite, R., Maneliene, Z., Capskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "FauI",
"organism": "Sphingobacterium multivorum RFL21",
"methylation_site": ""
},
"msp17i": {
"target_site": "GR^CGYC",
"name": "Msp17I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Madina, T.A., Rechkunova, N.I., Repin, V.E., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AcyI",
"organism": "Micrococcus species 17",
"methylation_site": ""
},
"cfr20i": {
"target_site": "CCWGG",
"name": "Cfr20I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL20",
"methylation_site": ""
},
"taqii": {
"target_site": "GACCGA(11/9),CACCCA(11/9)",
"name": "TaqII",
"suppliers": [
"Molecular Biology Resources - CHIMERx (1/11)",
"EURx Ltd. (1/11)"
],
"source": "J.I. Harris",
"references": [
"Barker, D., Hoff, M., Oliphant, A., White, R., (1984) Nucleic Acids Res., vol. 12, pp. 5567-5581."
],
"prototype": "",
"organism": "Thermus aquaticus YTI",
"methylation_site": "?(6)"
},
"mglii": {
"target_site": "?",
"name": "MglII",
"suppliers": [],
"source": "J. Davies",
"references": [
"Smith, D.I., Blattner, F.R., Davies, J., (1976) Nucleic Acids Res., vol. 3, pp. 343-353."
],
"prototype": "",
"organism": "Moraxella glueidi LG2",
"methylation_site": ""
},
"psc128i": {
"target_site": "?",
"name": "Psc128I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species NIVA-CYA128",
"methylation_site": ""
},
"ssl16216i": {
"target_site": "?",
"name": "Ssl16216I",
"suppliers": [],
"source": "FERM 16216",
"references": [
"Tomita, M., Toyama, K., Hiramatsu, A., Sho, K., Takahashi, T., Japanese Patent Office, 2000."
],
"prototype": "",
"organism": "Streptococcus salivarius subsp. thermophilus",
"methylation_site": ""
},
"eaepi": {
"target_site": "CTGCAG",
"name": "EaePI",
"suppliers": [],
"source": "ATCC 15038",
"references": [
"Parker, P., Schildkraut, I., Unpublished observations."
],
"prototype": "PstI",
"organism": "Enterobacter aerogenes",
"methylation_site": ""
},
"eari": {
"target_site": "CTCTTC(1/4)",
"name": "EarI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 450",
"references": [
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., Morgan, R.D., (1988) Nucleic Acids Res., vol. 16, pp. 9872.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chan, S.-h., International Patent Office, 2009."
],
"prototype": "Ksp632I",
"organism": "Enterobacter aerogenes",
"methylation_site": "?(4)"
},
"r2.ssu11318i": {
"target_site": "GATC",
"name": "R2.Ssu11318I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis NIAH11318",
"methylation_site": ""
},
"mnli": {
"target_site": "CCTC(7/6)",
"name": "MnlI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 17953",
"references": [
"Brinkley, P., Bautista, D.S., Graham, F.L., (1991) Gene, vol. 100, pp. 267-268.",
"Harasimowicz-Slowinska, R.I., Skowron, P.M., Unpublished observations.",
"Kriukiene, E., Lubiene, J., Lagunavicius, A., Lubys, A., (2005) Biochim. Biophys. Acta, vol. 1751, pp. 194-204.",
"Schildkraut, I., Unpublished observations.",
"Vitkute, J., Stankevicius, K., Tamulaitiene, G., Maneliene, Z., Timinskas, A., Berg, D.E., Janulaitis, A., (2001) J. Bacteriol., vol. 183, pp. 443-450.",
"Zabeau, M., Greene, R., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Moraxella nonliquefaciens",
"methylation_site": "1(5)"
},
"pflai": {
"target_site": "CGCG",
"name": "PflAI",
"suppliers": [],
"source": "ATCC 17582",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Pseudomonas fluorescens type A",
"methylation_site": ""
},
"bpii": {
"target_site": "GAAGAC(2/6)",
"name": "BpiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Jurgelyte, R., Maniusyte, V., Lazareviciute, L., Maneliene, Z., Kiuduliene, E., Norgeliene, D., Kvietkiene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus pumilus sw 4-3",
"methylation_site": ""
},
"bspaaiii": {
"target_site": "G^GATCC",
"name": "BspAAIII",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Alzhanov, D.T., Alzhanova, D.V., Zheleznaya, L.A., Matvienko, N.I., (1998) Biokhimiia, vol. 63, pp. 636-645."
],
"prototype": "BamHI",
"organism": "Bacillus species AA",
"methylation_site": ""
},
"bfi105i": {
"target_site": "GGNCC",
"name": "Bfi105I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "AsuI",
"organism": "Bacillus firmus 105",
"methylation_site": ""
},
"bcni": {
"target_site": "CC^SGG",
"name": "BcnI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Klimasauskas, S., Petrusyte, M., Butkus, V., (1983) FEBS Lett., vol. 161, pp. 131-134.",
"Janulaitis, A., Petrusyte, M.A., Jaskeleviciene, B.P., Krayev, A.S., Skryabin, K.G., Bayev, A.A., (1982) FEBS Lett., vol. 137, pp. 178-180.",
"Janulaitis, A., Petrusyte, M.P., Jaskeleviciene, B.P., Krayev, A.S., Skryabin, K.G., Bayev, A.A., (1981) Dokl. Akad. Nauk., vol. 257, pp. 749-750.",
"Klimasauskas, S., Butkus, V., Janulaitis, A., (1987) Mol. Biol. (Mosk), vol. 21, pp. 87-92.",
"Petrusyte, M.P., Janulaitis, A., (1981) Bioorg. Khim., vol. 7, pp. 1885-1887."
],
"prototype": "CauII",
"organism": "Bacillus centrosporus RFL1",
"methylation_site": "2(4)"
},
"bstnsii": {
"target_site": "CYCGRG",
"name": "BstNSII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Bacillus stearothermophilus 1161NS",
"methylation_site": ""
},
"bseb631ii": {
"target_site": "AGATCT",
"name": "BseB631II",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BglII",
"organism": "Bacillus species 6-3-1",
"methylation_site": ""
},
"saui": {
"target_site": "CC^TNAGG",
"name": "SauI",
"suppliers": [],
"source": "J. Timko",
"references": [
"Timko, J., Horwitz, A.H., Zelinka, J., Wilcox, G., (1981) J. Bacteriol., vol. 145, pp. 873-877."
],
"prototype": "",
"organism": "Streptomyces aureofaciens IKA 18/4",
"methylation_site": ""
},
"hinguii": {
"target_site": "GGATG",
"name": "HinGUII",
"suppliers": [],
"source": "J. Chirikjian",
"references": [
"Nardone, G., Blakesley, R., (1981) Fed. Proc., vol. 40, pp. 1848.",
"Smith, L., Blakesley, R., Chirikjian, J., Unpublished observations.",
"Tolstoshev, C.M., Blakesley, R.W., (1982) Nucleic Acids Res., vol. 10, pp. 1-17."
],
"prototype": "FokI",
"organism": "Haemophilus influenzae GU",
"methylation_site": ""
},
"bstmbi": {
"target_site": "^GATC",
"name": "BstMBI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Kovalenko, K., Kileva, E.V., Mezentseva, N.V., Mikhnenkova, N.A., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus MB",
"methylation_site": ""
},
"esalhci": {
"target_site": "GATC",
"name": "EsaLHCI",
"suppliers": [],
"source": "R.D. Morgan",
"references": [
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "MboI",
"organism": "Environmental sample LHC",
"methylation_site": "4(4)"
},
"eco211i": {
"target_site": "GRGCYC",
"name": "Eco211I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL211",
"methylation_site": ""
},
"btspi": {
"target_site": "GGGTC",
"name": "BtsPI",
"suppliers": [],
"source": "NEB 1507",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "SimI",
"organism": "Bacillus thermosphaericus",
"methylation_site": ""
},
"ksp632i": {
"target_site": "CTCTTC(1/4)",
"name": "Ksp632I",
"suppliers": [],
"source": "DSM 4196",
"references": [
"Bolton, B.J., Schmitz, G.G., Jarsch, M., Comer, M.J., Kessler, C., (1988) Gene, vol. 66, pp. 31-43.",
"Tsukahara, S., Yamakawa, H., Takai, K., Takaku, H., (1994) Nucleosides and Nucleotides, vol. 13, pp. 1617-1626."
],
"prototype": "",
"organism": "Kluyvera species 632",
"methylation_site": ""
},
"ainii": {
"target_site": "GGATCC",
"name": "AinII",
"suppliers": [],
"source": "CCAP 1446/1a",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Anabaena inequalis",
"methylation_site": ""
},
"maeiii": {
"target_site": "^GTNAC",
"name": "MaeIII",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "K.O. Stetter",
"references": [
"Schmid, K., Thomm, M., Laminet, A., Laue, F.G., Kessler, C., Stetter, K.O., Schmitt, R., (1984) Nucleic Acids Res., vol. 12, pp. 2619-2628."
],
"prototype": "",
"organism": "Methanococcus aeolicus PL-15/H",
"methylation_site": ""
},
"aspbi": {
"target_site": "CYCGRG",
"name": "AspBI",
"suppliers": [],
"source": "ATCC 29151",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaI",
"organism": "Anabaena species",
"methylation_site": ""
},
"vpak11ai": {
"target_site": "^GGWCC",
"name": "VpaK11AI",
"suppliers": [],
"source": "S. Matsushita",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus 1743",
"methylation_site": ""
},
"bmui": {
"target_site": "ACTGGG(5/4)",
"name": "BmuI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Nayakshina, T.N., Mezentseva, N.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BfiI",
"organism": "Bacillus megaterium S87",
"methylation_site": ""
},
"btci": {
"target_site": "GATC",
"name": "BtcI",
"suppliers": [],
"source": "R.R. Azizbekyan",
"references": [
"Kuzin, A.I., Bolesnin, M.I., Smolyaninov, V.V., Azizbekyan, R.R., (1989) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 36-39."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis var canadensis",
"methylation_site": ""
},
"sinei": {
"target_site": "GGWCC",
"name": "SinEI",
"suppliers": [],
"source": "S. Horiuchi",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Salmonella infantis 85064",
"methylation_site": ""
},
"eco151i": {
"target_site": "CCGCGG",
"name": "Eco151I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Escherichia coli RFL151",
"methylation_site": ""
},
"drdii": {
"target_site": "GAACCA",
"name": "DrdII",
"suppliers": [],
"source": "NEB 479",
"references": [
"Polisson, C., Murray, R.G.E., Unpublished observations."
],
"prototype": "",
"organism": "Deinococcus radiodurans",
"methylation_site": ""
},
"asp3065i": {
"target_site": "AAGCTT",
"name": "Asp3065I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Bolton, B.J., Schmitz, G., Jarsch, M., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Alcaligenes species 3065",
"methylation_site": ""
},
"eco237i": {
"target_site": "GAATTC",
"name": "Eco237I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Escherichia coli RFL237",
"methylation_site": ""
},
"sthji": {
"target_site": "GGTACC",
"name": "SthJI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY225",
"methylation_site": ""
},
"msai": {
"target_site": "GGCGCC",
"name": "MsaI",
"suppliers": [],
"source": "NRRL B16084",
"references": [
"Klein, S., Roberts, R.J., Unpublished observations."
],
"prototype": "NarI",
"organism": "Micromonospora saitamica",
"methylation_site": ""
},
"blei": {
"target_site": "?",
"name": "BleI",
"suppliers": [],
"source": "MTCC 1689",
"references": [
"Jutur, P.P., Reddy, A.R., (2007) Microbiol. Res., vol. 162, pp. 378-383."
],
"prototype": "",
"organism": "Bacillus lentus 1689",
"methylation_site": ""
},
"tsp514i": {
"target_site": "TCCGGA",
"name": "Tsp514I",
"suppliers": [],
"source": "ITI 355",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Thermus species",
"methylation_site": ""
},
"uba1388i": {
"target_site": "GGCC",
"name": "Uba1388I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1388",
"methylation_site": ""
},
"ecoei": {
"target_site": "GAGNNNNNNNATGC",
"name": "EcoEI",
"suppliers": [],
"source": "N.E. Murray",
"references": [
"Cowan, G.M., Gann, A.A.F., Murray, N.E., (1989) Cell, vol. 56, pp. 103-109.",
"Fuller-Pace, F.V., Cowan, G.M., Murray, N.E., (1985) J. Mol. Biol., vol. 186, pp. 65-75.",
"Murray, N.E., Daniel, A.S., Cowan, G.M., Sharp, P.M., (1993) Mol. Microbiol., vol. 9, pp. 133-143."
],
"prototype": "",
"organism": "Escherichia coli A58",
"methylation_site": "?(6)"
},
"bsp127i": {
"target_site": "ATCGAT",
"name": "Bsp127I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species RFL127",
"methylation_site": ""
},
"ngobi": {
"target_site": "RGCGCY",
"name": "NgoBI",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Stein, D.C., Gunn, J.S., Radlinska, M., Piekarowicz, A., (1995) Gene, vol. 157, pp. 19-22."
],
"prototype": "HaeII",
"organism": "Neisseria gonorrhoeae WR302",
"methylation_site": "?(5)"
},
"gdii": {
"target_site": "AGG^CCT",
"name": "GdiI",
"suppliers": [],
"source": "IAM 1814",
"references": [
"Van Montagu, M., Unpublished observations."
],
"prototype": "StuI",
"organism": "Gluconobacter dioxyacetonicus",
"methylation_site": ""
},
"styd4i": {
"target_site": "^CCNGG",
"name": "StyD4I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "E.S. Anderson",
"references": [
"Miyahara, M., Ishiwata, N., Yoshida, Y., (1997) Biol. Pharm. Bull., vol. 20, pp. 201-203.",
"Miyahara, M., Mise, K., (1993) Gene, vol. 129, pp. 83-86."
],
"prototype": "ScrFI",
"organism": "Salmonella typhi D4",
"methylation_site": "?(5)"
},
"saii": {
"target_site": "GGGTC",
"name": "SaiI",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Gaigalas, M., Capskaja, L., Norgeliene, D., Petrusyte, M., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "SimI",
"organism": "Staphylococcus auricularis RFL18",
"methylation_site": ""
},
"bspkt8i": {
"target_site": "A^AGCTT",
"name": "BspKT8I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Chernov, A.V., Zheleznaya, L.A., Matvienko, N.I., (1995) Biokhimiia, vol. 60, pp. 1318-1325."
],
"prototype": "HindIII",
"organism": "Bacillus species KT8",
"methylation_site": ""
},
"hpyf22iii": {
"target_site": "TCNNGA",
"name": "HpyF22III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy178III",
"organism": "Helicobacter pylori RFL22",
"methylation_site": ""
},
"bsp2362i": {
"target_site": "GGCC",
"name": "Bsp2362I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus 2362",
"methylation_site": ""
},
"bth1141i": {
"target_site": "GATC",
"name": "Bth1141I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. pondicheriensis",
"methylation_site": ""
},
"hpy166iii": {
"target_site": "CCTC",
"name": "Hpy166III",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "MnlI",
"organism": "Helicobacter pylori J166",
"methylation_site": ""
},
"cvni": {
"target_site": "CC^TNAGG",
"name": "CvnI",
"suppliers": [],
"source": "G.C. Grosveld",
"references": [
"Grosveld, G.C., Unpublished observations."
],
"prototype": "SauI",
"organism": "Chromatium vinosum",
"methylation_site": ""
},
"ubapi": {
"target_site": "CGAACG",
"name": "UbaPI",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Padegimiene, E., Petrusyte, M., Capskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Unidentified bacteria BPr-325",
"methylation_site": ""
},
"bspvi": {
"target_site": "GAAGAC",
"name": "BspVI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus species 6801",
"methylation_site": ""
},
"tsp1i": {
"target_site": "ACTGG(1/-1)",
"name": "Tsp1I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Karginov, V.A., Kovalenko, S.P., Kolesnikov, V.A., Chikaev, N.A., Serov, G.D., Repin, V.E., Unpublished observations."
],
"prototype": "BsrI",
"organism": "Thermus species strain 1",
"methylation_site": ""
},
"eclii": {
"target_site": "CCWGG",
"name": "EclII",
"suppliers": [],
"source": "H. Hartmann",
"references": [
"Hartmann, H., Goebel, W., (1977) FEBS Lett., vol. 80, pp. 285-287."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae",
"methylation_site": ""
},
"salhi": {
"target_site": "GATC",
"name": "SalHI",
"suppliers": [],
"source": "ATCC 12757",
"references": [
"Hall, D., Morgan, R., Unpublished observations."
],
"prototype": "MboI",
"organism": "Streptomyces albulus",
"methylation_site": ""
},
"bbvaiii": {
"target_site": "T^CCGGA",
"name": "BbvAIII",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Samko, O.T., Anikeitcheva, N.V., Kalugin, A.A., Khoroshutina, E.B., Plutalov, O.V., Birikh, K.R., Berlin, Y.A., (1994) Bioorg. Khim., vol. 20, pp. 1334-1341."
],
"prototype": "BspMII",
"organism": "Bacillus brevis A",
"methylation_site": ""
},
"sspm1i": {
"target_site": "TACGTA",
"name": "SspM1I",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "SnaBI",
"organism": "Spirulina species M1",
"methylation_site": ""
},
"mavi": {
"target_site": "C^TCGAG",
"name": "MavI",
"suppliers": [],
"source": "J.T. Crawford",
"references": [
"McDermott, P.F., Crawford, J.T., (1989) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 89, pp. 167."
],
"prototype": "XhoI",
"organism": "Mycobacterium avium",
"methylation_site": ""
},
"esphk26i": {
"target_site": "TCCGGA",
"name": "EspHK26I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Enterobacter species N26",
"methylation_site": ""
},
"cfai": {
"target_site": "R^AATTY",
"name": "CfaI",
"suppliers": [],
"source": "Vivantis 216",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "ApoI",
"organism": "Curtobacterium flaccumfaciens",
"methylation_site": ""
},
"rlu3i": {
"target_site": "GGNNCC",
"name": "Rlu3I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Rhodococcus luteus RFL3",
"methylation_site": ""
},
"hpyhi": {
"target_site": "CTNAG",
"name": "HpyHI",
"suppliers": [],
"source": "J. Gordon",
"references": [
"Oh, J.D., Kling-Backhed, H., Giannakis, M., Xu, J., Fulton, R.S., Fulton, L.A., Cordum, H.S., Wang, C., Elliott, G., Edwards, J., Mardis, E.R., Engstrand, L.G., Gordon, J.I., (2006) Proc. Natl. Acad. Sci. U. S. A., vol. 103, pp. 9999-10004.",
"Zheng, Y., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori HPAG1",
"methylation_site": ""
},
"mvsi": {
"target_site": "GGTACC",
"name": "MvsI",
"suppliers": [],
"source": "IMV 3017",
"references": [
"Romanovskaya, V.A., Alexeyev, M.F., Gunkovskaya, N.V., Stolyar, S.M., Shatohina, E.S., Malashenko, Y.R., (1992) Mikrobiol. Zh., vol. 54, pp. 32-39."
],
"prototype": "KpnI",
"organism": "Methylovarius species",
"methylation_site": ""
},
"uth555i": {
"target_site": "GGCC",
"name": "Uth555I",
"suppliers": [],
"source": "FZ 555",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium",
"methylation_site": ""
},
"bpvui": {
"target_site": "CGAT^CG",
"name": "BpvUI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 14",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Bacillus pumilus VU",
"methylation_site": ""
},
"gcei": {
"target_site": "CCGC^GG",
"name": "GceI",
"suppliers": [],
"source": "IFO 3262",
"references": [
"Hiraoka, N., Kita, K., Nakajima, H., Kimizuka, F., Obayashi, A., (1985) J. Ferment. Technol., vol. 63, pp. 151-157."
],
"prototype": "SacII",
"organism": "Gluconobacter cerinus",
"methylation_site": ""
},
"uba1373i": {
"target_site": "GGWCC",
"name": "Uba1373I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1373",
"methylation_site": ""
},
"rflfi": {
"target_site": "G^TCGAC",
"name": "RflFI",
"suppliers": [],
"source": "B.A. White",
"references": [
"Antonopoulos, D.A., White, B.A., Unpublished observations.",
"Morrison, M., Mackie, R.I., White, B.A., (1992) Appl. Environ. Microbiol., vol. 58, pp. 66-69."
],
"prototype": "SalI",
"organism": "Ruminococcus flavefaciens FD-1",
"methylation_site": ""
},
"m.ncrni": {
"target_site": "?",
"name": "M.NcrNI",
"suppliers": [],
"source": "E.U. Selker",
"references": [
"Kouzminova, E., Selker, E.U., (2001) EMBO J., vol. 20, pp. 4309-4323."
],
"prototype": "",
"organism": "Neurospora crassa N",
"methylation_site": "?(5)"
},
"bstz10i": {
"target_site": "CCNNGG",
"name": "BstZ10I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "SecI",
"organism": "Bacillus stearothermophilus 35L",
"methylation_site": ""
},
"ssl16217i": {
"target_site": "?",
"name": "Ssl16217I",
"suppliers": [],
"source": "FERM 16217",
"references": [
"Tomita, M., Toyama, K., Hiramatsu, A., Sho, K., Takahashi, T., Japanese Patent Office, 2000."
],
"prototype": "",
"organism": "Streptococcus salivarius subsp. thermophilus",
"methylation_site": ""
},
"pae36i": {
"target_site": "CCGCGG",
"name": "Pae36I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Pseudomonas aeruginosa RFL36",
"methylation_site": ""
},
"alw44i": {
"target_site": "G^TGCAC",
"name": "Alw44I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Jagelavicius, M., Bitinaite, J., Maneliene, Z., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "ApaLI",
"organism": "Acinetobacter lwoffi RFL44",
"methylation_site": ""
},
"ncai": {
"target_site": "GANTC",
"name": "NcaI",
"suppliers": [],
"source": "NRCC 31003",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Neisseria caviae",
"methylation_site": ""
},
"bsp105i": {
"target_site": "^GATC",
"name": "Bsp105I",
"suppliers": [],
"source": "Q.-L. Li",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus 105",
"methylation_site": ""
},
"pinai": {
"target_site": "A^CCGGT",
"name": "PinAI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Roche Applied Science (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)",
"EURx Ltd. (1/11)"
],
"source": "M.M. Wijdenbosch",
"references": [
"Groot, E., Unpublished observations."
],
"prototype": "AgeI",
"organism": "Pseudomonas inequalis",
"methylation_site": ""
},
"bst12i": {
"target_site": "GCAGC(8/12)",
"name": "Bst12I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BbvI",
"organism": "Bacillus stearothermophilus 12",
"methylation_site": ""
},
"nmeri": {
"target_site": "CAG^CTG",
"name": "NmeRI",
"suppliers": [],
"source": "CAMB 2584",
"references": [
"Mushtaq, R., Sohail, A., Riazuddin, S., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Neisseria meningitidis",
"methylation_site": ""
},
"bsemii": {
"target_site": "CTCAG(10/8)",
"name": "BseMII",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Jurenaite-Urbanaviciene, S., Kazlauskiene, R., Urbelyte, V., Maneliene, Z., Petrusyte, M., Lubys, A., Janulaitis, A., (2001) Nucleic Acids Res., vol. 29, pp. 895-903.",
"Maneliene, Z., Butkus, V., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus Isl 15-111",
"methylation_site": "4(6)"
},
"sciaii": {
"target_site": "CAGCTG",
"name": "SciAII",
"suppliers": [],
"source": "ATCC 27175",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Synechocystis species",
"methylation_site": ""
},
"bsebi": {
"target_site": "CC^WGG",
"name": "BseBI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "V. Bouriotis",
"references": [
"Rina, M., Tsigos, I., Karagouni, A., Pagomenou, M., Bouriotis, V., (1991) Nucleic Acids Res., vol. 19, pp. 4776."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus",
"methylation_site": ""
},
"ali12257i": {
"target_site": "GGATCC",
"name": "Ali12257I",
"suppliers": [],
"source": "IFO 12257",
"references": [
"Yamada, Y., Sasaki, J., (1984) J. Gen. Appl. Microbiol., vol. 30, pp. 309-312."
],
"prototype": "BamHI",
"organism": "Acetobacter liquefaciens",
"methylation_site": ""
},
"gsai": {
"target_site": "CCCAGC(-1/-5)",
"name": "GsaI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Mikhnenkova, N.A., Kileva, E.V., Tarasova, G.V., Gonchar, D.A., Akishev, A.G., Okhapkina, S.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BseYI",
"organism": "Geobacillus stearothermophilus Y",
"methylation_site": ""
},
"eco206i": {
"target_site": "CCWGG",
"name": "Eco206I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL206",
"methylation_site": ""
},
"uban5i": {
"target_site": "CTAG",
"name": "UbaN5I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "MaeI",
"organism": "Unidentified bacterium CN8-01, Nicaragua",
"methylation_site": ""
},
"bstoz616i": {
"target_site": "GGGAC(10/14)",
"name": "BstOZ616I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus stearothermophilus OZ616",
"methylation_site": ""
},
"bfi458i": {
"target_site": "GGCC",
"name": "Bfi458I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus firmus 458",
"methylation_site": ""
},
"bse126i": {
"target_site": "GGCC",
"name": "Bse126I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "HaeIII",
"organism": "Bacillus species 12-6",
"methylation_site": ""
},
"btumi": {
"target_site": "TCG^CGA",
"name": "BtuMI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 61",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "NruI",
"organism": "Brevibacillus thermoruber",
"methylation_site": ""
},
"eco155i": {
"target_site": "GGTCTC",
"name": "Eco155I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL155",
"methylation_site": ""
},
"gobai": {
"target_site": "AGGCCT",
"name": "GobAI",
"suppliers": [],
"source": "Glaxo 2323C",
"references": [
"Earle-Hughes, J., Roberts, R.J., Unpublished observations."
],
"prototype": "StuI",
"organism": "Geodermatophilus obscurus",
"methylation_site": ""
},
"mspv281i": {
"target_site": "GWGCW^C",
"name": "MspV281I",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Waleron, K., Piechula, S., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Myxosarcinia sp V/281",
"methylation_site": ""
},
"eco51i": {
"target_site": "GGTCTC",
"name": "Eco51I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL51",
"methylation_site": ""
},
"cfr54i": {
"target_site": "GGNCC",
"name": "Cfr54I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Jagelavicius, M., Bitinaite, J., Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL54",
"methylation_site": ""
},
"sliii": {
"target_site": "?",
"name": "SliII",
"suppliers": [],
"source": "R.H. Baltz",
"references": [
"Matsushima, P., Baltz, R.H., (1989) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 89, pp. 361."
],
"prototype": "",
"organism": "Streptomyces lipmanii",
"methylation_site": ""
},
"eco188i": {
"target_site": "AAGCTT",
"name": "Eco188I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Escherichia coli RFL188",
"methylation_site": ""
},
"hin6i": {
"target_site": "G^CGC",
"name": "Hin6I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "HhaI",
"organism": "Haemophilus influenzae RFL6",
"methylation_site": ""
},
"bbsi": {
"target_site": "GAAGAC(2/6)",
"name": "BbsI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 573",
"references": [
"Story, C.M., Morgan, R., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus brevis",
"methylation_site": ""
},
"cvici": {
"target_site": "GANTC",
"name": "CviCI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Xia, Y., Burbank, D.E., Van Etten, J.L., (1986) Nucleic Acids Res., vol. 14, pp. 6017-6030."
],
"prototype": "HinfI",
"organism": "Chlorella strain NC64A (NE-8A)",
"methylation_site": ""
},
"uba1450i": {
"target_site": "GGCC",
"name": "Uba1450I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1450",
"methylation_site": ""
},
"rma496i": {
"target_site": "CTAG",
"name": "Rma496I",
"suppliers": [],
"source": "ITI 496",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"uba1155i": {
"target_site": "GGCC",
"name": "Uba1155I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1155",
"methylation_site": ""
},
"nsiai": {
"target_site": "GATC",
"name": "NsiAI",
"suppliers": [],
"source": "ATCC 9913",
"references": [
"Comb, D.G., Unpublished observations."
],
"prototype": "MboI",
"organism": "Neisseria sicca",
"methylation_site": ""
},
"bstz6i": {
"target_site": "CCTNAGG",
"name": "BstZ6I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "SauI",
"organism": "Bacillus stearothermophilus 25Q",
"methylation_site": ""
},
"eco164i": {
"target_site": "YGGCCR",
"name": "Eco164I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Escherichia coli RFL164",
"methylation_site": ""
},
"kpl79i": {
"target_site": "CGATCG",
"name": "Kpl79I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Klebsiella planticola RFL79",
"methylation_site": ""
},
"mstii": {
"target_site": "CC^TNAGG",
"name": "MstII",
"suppliers": [],
"source": "D.G. Comb",
"references": [
"Schildkraut, I., Unpublished observations."
],
"prototype": "SauI",
"organism": "Microcoleus species",
"methylation_site": ""
},
"hpyf72i": {
"target_site": "GGCC",
"name": "HpyF72I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL72",
"methylation_site": ""
},
"hgihi": {
"target_site": "G^GYRCC",
"name": "HgiHI",
"suppliers": [],
"source": "J.H. Parish",
"references": [
"Whitehead, P.R., Jacobs, D., Brown, N.L., (1986) Nucleic Acids Res., vol. 14, pp. 7031-7045."
],
"prototype": "HgiCI",
"organism": "Herpetosiphon giganteus HP1049",
"methylation_site": ""
},
"hpyf23i": {
"target_site": "TCGA",
"name": "HpyF23I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL23",
"methylation_site": ""
},
"nspli": {
"target_site": "TGCGCA",
"name": "NspLI",
"suppliers": [],
"source": "C.P. Wolk",
"references": [
"Karreman, C., de Waard, A., Unpublished observations."
],
"prototype": "MstI",
"organism": "Nostoc species UM-3",
"methylation_site": ""
},
"senpci": {
"target_site": "CCGCGG",
"name": "SenpCI",
"suppliers": [],
"source": "I. Rychlik",
"references": [
"Gregorova, D., Pravcova, M., Karpiskova, R., Rychlik, I., (2002) FEMS Microbiol. Lett., vol. 214, pp. 195-198."
],
"prototype": "SacII",
"organism": "Salmonella enteritidis plasmid pC",
"methylation_site": "?(5)"
},
"asp28i": {
"target_site": "?",
"name": "Asp28I",
"suppliers": [],
"source": "A.M. Kulba",
"references": [
"Kulba, A.M., Elgammudi, A.A., (1997) Vestn. Beloruss. Gos. Univ., vol. 2, pp. 47-49."
],
"prototype": "",
"organism": "Aeromonas species",
"methylation_site": ""
},
"bstxi": {
"target_site": "CCANNNNN^NTGG",
"name": "BstXI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations.",
"Nwankwo, D., Unpublished observations.",
"Skowron, P.M., Rutkowska, S.M., Harasimowicz-Slowinska, R.I., Unpublished observations.",
"Wise, R., Schildkraut, I., Unpublished observations.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chan, S.-h., International Patent Office, 2009.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus X1",
"methylation_site": ""
},
"blsi": {
"target_site": "GCN^GC",
"name": "BlsI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Tomilova, J.E., Chmuzh, E.V., Sokolova, O.O., Dedkov, V.S., Degtyarev, S.K., Russian Patent Office, 2008.",
"Chernukhin, V.A., Tomilova, J.E., Chmuzh, E.V., Sokolova, O.O., Dedkov, V.S., Degtyarev, S.K., (2007) Ovchinnikov Bull. Biotechnol. Phys. Chem. Biol., vol. 3, pp. 28-33."
],
"prototype": "BisI",
"organism": "Bacillus simplex 23",
"methylation_site": ""
},
"eco196ii": {
"target_site": "GGNCC",
"name": "Eco196II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Escherichia coli RFL196",
"methylation_site": ""
},
"smo40529i": {
"target_site": "GCCGGC",
"name": "Smo40529I",
"suppliers": [],
"source": "DSM 40529",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Streptomyces moderatus",
"methylation_site": ""
},
"mlui": {
"target_site": "A^CGCGT",
"name": "MluI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "IFO 12992",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Sugisaki, H., Kanazawa, S., (1981) Gene, vol. 16, pp. 73-78."
],
"prototype": "",
"organism": "Micrococcus luteus",
"methylation_site": "?(4)"
},
"ecot14i": {
"target_site": "C^CWWGG",
"name": "EcoT14I",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "N. Terakado",
"references": [
"Yoshida, Y., Mise, K., Unpublished observations."
],
"prototype": "StyI",
"organism": "Escherichia coli TB14",
"methylation_site": ""
},
"cviaii": {
"target_site": "C^ATG",
"name": "CviAII",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "J.L. Van Etten",
"references": [
"Gurnon, J.R., Graves, M.V., Van Etten, J.L., Unpublished observations.",
"Zhang, Y., Nelson, M., Nietfeldt, J.W., Burbank, D.E., Van Etten, J.L., (1992) Nucleic Acids Res., vol. 20, pp. 5351-5356."
],
"prototype": "NlaIII",
"organism": "Chlorella strain NC64A (PBCV-1)",
"methylation_site": "2(6)"
},
"pgli": {
"target_site": "GCCGGC",
"name": "PglI",
"suppliers": [],
"source": "J.V. Leary",
"references": [
"Leary, J.V., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Pseudomonas glycinae",
"methylation_site": ""
},
"bstxii": {
"target_site": "GATC",
"name": "BstXII",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus X1",
"methylation_site": ""
},
"eco1446i": {
"target_site": "GAGNNNNNNNGTCA",
"name": "Eco1446I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoAI",
"organism": "Escherichia coli EC1446",
"methylation_site": ""
},
"mmei": {
"target_site": "TCCRAC(20/18)",
"name": "MmeI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "W.J. Brammar",
"references": [
"Boyd, A.C., Charles, I.G., Keyte, J.W., Brammar, W.J., (1986) Nucleic Acids Res., vol. 14, pp. 5255-5274.",
"Morgan, R.D., Unpublished observations.",
"Tucholski, J., Zmijewski, J.W., Podhajska, A.J., (1998) Gene, vol. 223, pp. 293-302.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Methylophilus methylotrophus",
"methylation_site": "5(6)"
},
"bmgi": {
"target_site": "GKGCCC",
"name": "BmgI",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Markauskiene, J., Valickiene, D., Trinkunaite, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus megaterium E11-132",
"methylation_site": ""
},
"sfri": {
"target_site": "CCGCGG",
"name": "SfrI",
"suppliers": [],
"source": "ATCC 3355",
"references": [
"Shimotsu, H., Takahashi, H., Saito, H., (1980) Agric. Biol. Chem., vol. 44, pp. 1665-1666.",
"Takahashi, H., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces fradiae",
"methylation_site": ""
},
"uba1395i": {
"target_site": "GGCC",
"name": "Uba1395I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1395",
"methylation_site": ""
},
"suai": {
"target_site": "GG^CC",
"name": "SuaI",
"suppliers": [],
"source": "DSM 639",
"references": [
"Chen, L., Brugger, K., Skovgaard, M., Redder, P., She, Q., Torarinsson, E., Greve, B., Awayez, M., Zibat, A., Klenk, H.-P., Garrett, R.A., (2005) J. Bacteriol., vol. 187, pp. 4992-4999.",
"Grogan, D.W., (2003) J. Bacteriol., vol. 185, pp. 4657-4661.",
"Prangishvili, D.A., Chinchaladze, D.Z., Chelidze, M.G., (1987) Biotekhnologiya, vol. 3, pp. 440-446.",
"Prangishvili, D.A., Vashakidze, R.P., Chelidze, M.G., Gabriadze, I.Yu., (1985) FEBS Lett., vol. 192, pp. 57-60."
],
"prototype": "HaeIII",
"organism": "Sulfolobus acidocaldarius",
"methylation_site": "3(4)"
},
"bsplaii": {
"target_site": "TT^CGAA",
"name": "BspLAII",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Shiryaev, S.A., Zheleznaya, L.A., Matvienko, N.I., (1997) Biokhimiia, vol. 62, pp. 280-290."
],
"prototype": "AsuII",
"organism": "Bacillus species LA",
"methylation_site": ""
},
"bsshii": {
"target_site": "G^CGCGC",
"name": "BssHII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 49820",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations.",
"Schildkraut, I., Greenough, L., Unpublished observations.",
"Schumann, J., Willert, J., Wild, C., Waler, J., Trautner, T.A., (1995) Gene, vol. 157, pp. 103-104.",
"Xu, S.-Y., Xiao, J.-P., Posfai, J., Maunus, R., Benner, J., (1997) Nucleic Acids Res., vol. 25, pp. 3991-3994."
],
"prototype": "BsePI",
"organism": "Bacillus stearothermophilus H3",
"methylation_site": "?(5)"
},
"ngoaii": {
"target_site": "GGCC",
"name": "NgoAII",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Hammond, A.W., Chatterjee, D.K., International Patent Office, 1991.",
"Lewis, L.A. et al., Unpublished observations.",
"Monaci, E., Masignani, V., Pizza, M., Fontana, M.R., Japanese Patent Office, 2004.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Roe, B., Song, L., Lin, S.-P., Yuan, X.-L., Clifton, S., Ducey, T., Lewis, L., Dyer, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(5)"
},
"baui": {
"target_site": "CACGAG(-5/-1)",
"name": "BauI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G268",
"references": [
"Maneliene, Z., Zakareviciene, L., Unpublished observations.",
"Vitkute, J., Nekrasiene, D., Lapcinskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BsiI",
"organism": "Bacillus aquaemaris RFL1",
"methylation_site": ""
},
"hinbiii": {
"target_site": "AAGCTT",
"name": "HinbIII",
"suppliers": [],
"source": "C.A. Hutchison",
"references": [
"Middleton, J.H., Stankus, P.V., Edgell, M.H., Hutchison, C.A. III, Unpublished observations.",
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Haemophilus influenzae Rb",
"methylation_site": ""
},
"uban16i": {
"target_site": "CCWGG",
"name": "UbaN16I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Huete-Perez, J.A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium HSJ-15-1-G, Nicaragua",
"methylation_site": ""
},
"ngoaiv": {
"target_site": "G^CCGGC",
"name": "NgoAIV",
"suppliers": [],
"source": "ATCC 700825",
"references": [
"Fontana, M.R., Pizza, M., Masignani, V., Monaci, E., European Patent Office, 2007.",
"Hammond, A.W., Chatterjee, D.K., Gerard, G.F., International Patent Office, 1991.",
"Hammond, A.W., Gerard, G.F., Campbell, J.H., Chatterjee, D.K., (1989) Nucleic Acids Res., vol. 17, pp. 3320.",
"Hu, A.-L.W., (1994) BRL Focus, vol. 15, pp. 42-43.",
"Lewis, L.A. et al., Unpublished observations.",
"Roe, B., Song, L., Lin, S.-P., Yuan, X.-L., Clifton, S., Ducey, T., Lewis, L., Dyer, D., Unpublished observations.",
"Zhu, P., Morelli, G., Achtman, M., (1999) Mol. Microbiol., vol. 33, pp. 635-650."
],
"prototype": "NaeI",
"organism": "Neisseria gonorrhoeae FA1090",
"methylation_site": "?(5)"
},
"hpyf9iii": {
"target_site": "ACNGT",
"name": "HpyF9III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL9",
"methylation_site": ""
},
"bshbi": {
"target_site": "GGCC",
"name": "BshBI",
"suppliers": [],
"source": "WHO/CCBC 1881",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"stysgi": {
"target_site": "TAANNNNNNRTCG",
"name": "StySGI",
"suppliers": [],
"source": "L.R. Bullas",
"references": [
"Kasarjian, J.K.A., Hidaka, M., Horiuchi, T., Iida, M., Ryu, J., (2004) Nucleic Acids Res., vol. 32."
],
"prototype": "",
"organism": "Salmonella gelsenkirchen",
"methylation_site": "3(6),-3(6)"
},
"uba1099i": {
"target_site": "GGNCC",
"name": "Uba1099I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Unidentified bacterium RFL1099",
"methylation_site": ""
},
"acii": {
"target_site": "CCGC(-3/-1)",
"name": "AciI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 577",
"references": [
"Heiter, D.F., Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Lunnen, K.D., Heiter, D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., Morgan, R.D., (1990) Nucleic Acids Res., vol. 18, pp. 5911.",
"Samuelson, J., Unpublished observations."
],
"prototype": "",
"organism": "Arthrobacter citreus",
"methylation_site": "1(5),-2(5)"
},
"uba1443i": {
"target_site": "CTTAAG",
"name": "Uba1443I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AflII",
"organism": "Unidentified bacterium RFL1443",
"methylation_site": ""
},
"hin8i": {
"target_site": "GRCGYC",
"name": "Hin8I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "AcyI",
"organism": "Haemophilus influenzae RFL8",
"methylation_site": ""
},
"vcho60i": {
"target_site": "?",
"name": "VchO60I",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "",
"organism": "Vibrio cholerae 195-75",
"methylation_site": ""
},
"sau22201i": {
"target_site": "?",
"name": "Sau22201I",
"suppliers": [],
"source": "J. Timko",
"references": [
"Timko, J., Matuskova, M., Zelinkova, E., Zelinka, J., (1978) Folia Microbiol. (Praha), vol. 23, pp. 243-245."
],
"prototype": "",
"organism": "Streptomyces aureofaciens IKA 22201",
"methylation_site": ""
},
"mchaii": {
"target_site": "GG^CC",
"name": "MchAII",
"suppliers": [],
"source": "A.K. Tyagi",
"references": [
"Shankar, S., Tyagi, A.K., (1993) Gene, vol. 132, pp. 119-123."
],
"prototype": "HaeIII",
"organism": "Mycobacterium chelonei",
"methylation_site": ""
},
"btsci": {
"target_site": "GGATG(2/0)",
"name": "BtsCI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1621",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Pan, X.S., Morgan, R., Unpublished observations.",
"Too, P.H., Zhu, Z., Chan, S.H., Xu, S.Y., (2010) Nucleic Acids Res., vol. 38, pp. 1294-1303.",
"Zhu, Z., Xu, S.-Y., Unpublished observations."
],
"prototype": "FokI",
"organism": "Bacillus thermosphaericus",
"methylation_site": ""
},
"cpfi": {
"target_site": "^GATC",
"name": "CpfI",
"suppliers": [],
"source": "R. Hansen",
"references": [
"Hansen, R., Unpublished observations."
],
"prototype": "MboI",
"organism": "Clostridium perfringens",
"methylation_site": ""
},
"bsp147i": {
"target_site": "GATC",
"name": "Bsp147I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL147",
"methylation_site": ""
},
"pma44i": {
"target_site": "CTGCAG",
"name": "Pma44I",
"suppliers": [],
"source": "CAMB 2550",
"references": [
"Sohail, A., Khan, E., Maqbool, T., Riazuddin, S., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas maltophilia",
"methylation_site": ""
},
"uba1177i": {
"target_site": "GATC",
"name": "Uba1177I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1177",
"methylation_site": ""
},
"rflfiii": {
"target_site": "CGCCAG",
"name": "RflFIII",
"suppliers": [],
"source": "B.A. White",
"references": [
"Dwinell, E.A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Ruminococcus flavefaciens FD-1",
"methylation_site": ""
},
"bst170ii": {
"target_site": "AAGCTT",
"name": "Bst170II",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Bacillus stearothermophilus D170",
"methylation_site": ""
},
"hpyf65i": {
"target_site": "ACNGT",
"name": "HpyF65I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL65",
"methylation_site": ""
},
"hpyf12i": {
"target_site": "ACNGT",
"name": "HpyF12I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL12",
"methylation_site": ""
},
"bluii": {
"target_site": "GGCC",
"name": "BluII",
"suppliers": [],
"source": "ATCC 15830",
"references": [
"Van Montagu, M., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Brevibacterium luteum",
"methylation_site": ""
},
"sau15i": {
"target_site": "GATC",
"name": "Sau15I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus RFL15",
"methylation_site": ""
},
"bspbs31i": {
"target_site": "GAAGAC(2/6)",
"name": "BspBS31I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kovalevskaya, N.P., Zelinskaya, N.V., Zheleznaya, L.A., Matvienko, N.I., (1993) Bioorg. Khim., vol. 19, pp. 1073-1076."
],
"prototype": "BbvII",
"organism": "Bacillus species BS31",
"methylation_site": ""
},
"uba1195i": {
"target_site": "ATCGAT",
"name": "Uba1195I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1195",
"methylation_site": ""
},
"hpyf6i": {
"target_site": "GGATG",
"name": "HpyF6I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "FokI",
"organism": "Helicobacter pylori RFL6",
"methylation_site": ""
},
"asp50hi": {
"target_site": "GAATGC(1/-1)",
"name": "Asp50HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Kobayashi, F., Dou, D., Nomura, Y., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Acidiphilium species 50H",
"methylation_site": ""
},
"hpyf73ii": {
"target_site": "TCGA",
"name": "HpyF73II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL73",
"methylation_site": ""
},
"asp703i": {
"target_site": "CTCGAG",
"name": "Asp703I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Kessler, C., Neumaier, P.S., Wolf, W., (1985) Gene, vol. 33, pp. 1-102."
],
"prototype": "XhoI",
"organism": "Achromobacter species 703",
"methylation_site": ""
},
"bsp143i": {
"target_site": "^GATC",
"name": "Bsp143I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL143",
"methylation_site": ""
},
"uba1258i": {
"target_site": "GGATCC",
"name": "Uba1258I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1258",
"methylation_site": ""
},
"m.vchk139i": {
"target_site": "GATC",
"name": "M.VchK139I",
"suppliers": [],
"source": "J. Reidl",
"references": [
"Ehara, M., Nguyen, M.B., Nguyen, T.D., Ngo, C.T., Le, H.T., Nguyen, T.H., Iwami, M., Unpublished observations.",
"Kapfhammer, D., Blass, J., Evers, S., Reidl, J., (2002) J. Bacteriol., vol. 184, pp. 6592-6601."
],
"prototype": "MboI",
"organism": "Vibrio cholerae phage K139",
"methylation_site": "2(6)"
},
"bsph106ii": {
"target_site": "GGCC",
"name": "BspH106II",
"suppliers": [],
"source": "ITI 106",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus species",
"methylation_site": ""
},
"hpyf73iv": {
"target_site": "GGNCC",
"name": "HpyF73IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Helicobacter pylori RFL73",
"methylation_site": ""
},
"hpyf26iii": {
"target_site": "TCGA",
"name": "HpyF26III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL26",
"methylation_site": ""
},
"eco1344i": {
"target_site": "AACNNNNNNGTGC",
"name": "Eco1344I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoKI",
"organism": "Escherichia coli EC1344",
"methylation_site": ""
},
"uba1290i": {
"target_site": "CCTNNNNNAGG",
"name": "Uba1290I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoNI",
"organism": "Unidentified bacterium RFL1290",
"methylation_site": ""
},
"uba1227i": {
"target_site": "CAGCTG",
"name": "Uba1227I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Unidentified bacterium RFL1227",
"methylation_site": ""
},
"sscl1i": {
"target_site": "G^ANTC",
"name": "SscL1I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Vasiljeva, L.Y., Zheleznaya, L.A., Matvienko, N.I., (1998) Biokhimiia, vol. 63, pp. 212-218.",
"Vasiljeva, L.Y., Zheleznaya, L.A., Matvienko, N.I., (2000) Biokhimiia, vol. 65, pp. 565-570."
],
"prototype": "HinfI",
"organism": "Staphylococcus species L1",
"methylation_site": "2(6)"
},
"ecl593i": {
"target_site": "CTGCAG",
"name": "Ecl593I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "PstI",
"organism": "Enterobacter cloacae 593",
"methylation_site": ""
},
"eco85i": {
"target_site": "CCNGG",
"name": "Eco85I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bagdonaviciute, V., Petrusyte, M., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Escherichia coli RFL85",
"methylation_site": ""
},
"hpyf50ii": {
"target_site": "TCNGA",
"name": "HpyF50II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL50",
"methylation_site": ""
},
"bspo4i": {
"target_site": "CAG^CTG",
"name": "BspO4I",
"suppliers": [],
"source": "ATCC 21536",
"references": [
"Moriya, S., Yanagawa, S., Aoki, N., Iwabuchi, M., Inoue, T., Ando, T., (1992) Nucleic Acids Res., vol. 20, pp. 3781."
],
"prototype": "PvuII",
"organism": "Bacillus species O-4",
"methylation_site": ""
},
"plii": {
"target_site": "GTGCAC",
"name": "PliI",
"suppliers": [],
"source": "ATCC 31099",
"references": [
"Klein, S., Roberts, R.J., Unpublished observations."
],
"prototype": "ApaLI",
"organism": "Pseudomonas lindbergii",
"methylation_site": ""
},
"clii": {
"target_site": "GGWCC",
"name": "CliI",
"suppliers": [],
"source": "UTEX 2014",
"references": [
"Karreman, C., de Waard, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Cylindrospermum lichenforme",
"methylation_site": ""
},
"psp33i": {
"target_site": "GTCGAC",
"name": "Psp33I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SalI",
"organism": "Pseudomonas species RFL33",
"methylation_site": ""
},
"uba1409i": {
"target_site": "GRGCYC",
"name": "Uba1409I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1409",
"methylation_site": ""
},
"ndeii": {
"target_site": "^GATC",
"name": "NdeII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"Promega Corporation (7/10)"
],
"source": "NRCC 31009",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Neisseria denitrificans",
"methylation_site": ""
},
"sfl2bi": {
"target_site": "CCWGG",
"name": "Sfl2bI",
"suppliers": [],
"source": "L.I. Solodukhina",
"references": [
"Solodukhina, L.I., Manuvakhova, M.S., Korotayev, A.I., (1989) Genetika, vol. 25, pp. 1571-1577."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 2a",
"methylation_site": ""
},
"bsp9002i": {
"target_site": "?",
"name": "Bsp9002I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "",
"organism": "Bacillus sphaericus 9002",
"methylation_site": ""
},
"bstji": {
"target_site": "GGCC",
"name": "BstJI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus stearothermophilus J460",
"methylation_site": ""
},
"ubaf12i": {
"target_site": "CTACNNNGTC",
"name": "UbaF12I",
"suppliers": [],
"source": "Fermentas G333",
"references": [
"Vitkute, J., Lauciuniene, N., Riauba, L., Capskaja, L., Norgeliene, D., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Unidentified bacterium F12",
"methylation_site": ""
},
"cstmi": {
"target_site": "AAGGAG(20/18)",
"name": "CstMI",
"suppliers": [],
"source": "A. Tauch",
"references": [
"Morgan, R.D., Bhatia, T., US Patent Office, 2005.",
"Morgan, R.D., Bhatia, T., US Patent Office, 2007.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Corynebacterium striatum M82B",
"methylation_site": "?(6)"
},
"agsi": {
"target_site": "TTS^AA",
"name": "AgsI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Kileva, E.V., Tarasova, G.V., Dedkov, V.S., Mikhnenkova, N.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "",
"organism": "Agrococcus species 25",
"methylation_site": ""
},
"esp9i": {
"target_site": "GGYRCC",
"name": "Esp9I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL9",
"methylation_site": ""
},
"afliii": {
"target_site": "A^CRYGT",
"name": "AflIII",
"suppliers": [
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)"
],
"source": "CCAP 1403/13f",
"references": [
"Lunnen, K.D., Moran, L., Slatko, B.E., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Whitehead, P.R., Brown, N.L., (1985) J. Gen. Microbiol., vol. 131, pp. 951-958."
],
"prototype": "",
"organism": "Anabaena flos-aquae",
"methylation_site": "2(4)"
},
"nsudi": {
"target_site": "GATC",
"name": "NsuDI",
"suppliers": [],
"source": "ATCC 19243",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations."
],
"prototype": "DpnI",
"organism": "Neisseria subflava",
"methylation_site": ""
},
"bstbai": {
"target_site": "YAC^GTR",
"name": "BstBAI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsaAI",
"organism": "Bacillus stearothermophilus BA",
"methylation_site": ""
},
"gdiii": {
"target_site": "CGGCCR(-5/-1)",
"name": "GdiII",
"suppliers": [],
"source": "IAM 1814",
"references": [
"Van Montagu, M., Unpublished observations."
],
"prototype": "",
"organism": "Gluconobacter dioxyacetonicus",
"methylation_site": ""
},
"bsobi": {
"target_site": "C^YCGRG",
"name": "BsoBI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations.",
"Fomenkov, A., Dila, D.K., Raleigh, E.A., Xu, S.-Y., European Patent Office, 2004.",
"Ruan, H., Lunnen, K.D., Scott, M.E., Moran, L.S., Slatko, B.E., Pelletier, J.J., Hess, E.J., Benner, J., Wilson, G.G., Xu, S.-Y., (1996) Mol. Gen. Genet., vol. 252, pp. 695-699.",
"Spargo, C.A., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Bacillus stearothermophilus JN2091",
"methylation_site": "?(4)"
},
"mlu31i": {
"target_site": "TGG^CCA",
"name": "Mlu31I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Serov, G.D., Puchkova, L.I., Kolesnikov, V.A., Repin, V.E., Unpublished observations."
],
"prototype": "BalI",
"organism": "Micrococcus luteus 31",
"methylation_site": ""
},
"asp697i": {
"target_site": "GGWCC",
"name": "Asp697I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Kessler, C., Neumaier, P.S., Wolf, W., (1985) Gene, vol. 33, pp. 1-102."
],
"prototype": "AvaII",
"organism": "Achromobacter species 697",
"methylation_site": ""
},
"bscoi": {
"target_site": "GCATGC",
"name": "BscOI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "SphI",
"organism": "Bacillus species 4319",
"methylation_site": ""
},
"bco10278i": {
"target_site": "GGATCC",
"name": "Bco10278I",
"suppliers": [],
"source": "NCIB 10278",
"references": [
"Leung, S.M., Chan, K.Y., Suen, Y.K., Shaw, P.C., (1989) Nucleic Acids Res., vol. 17, pp. 10133."
],
"prototype": "BamHI",
"organism": "Bacillus coagulans",
"methylation_site": ""
},
"bme142i": {
"target_site": "RGC^GCY",
"name": "Bme142I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Fomenkov, A.I., Kramarov, V.M., Andreev, L.V., Mochalov, V.V., Smolyaninov, V.V., Matvienko, N.I., (1988) Nucleic Acids Res., vol. 16, pp. 10399."
],
"prototype": "HaeII",
"organism": "Bacillus megaterium 142",
"methylation_site": ""
},
"eco1265i": {
"target_site": "TGANNNNNNNNTGCT",
"name": "Eco1265I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoBI",
"organism": "Escherichia coli ECL 1265",
"methylation_site": "?(6)"
},
"m.babi": {
"target_site": "GANTC",
"name": "M.BabI",
"suppliers": [],
"source": "L. Shapiro",
"references": [
"Paulsen, I. et al., (2002) Proc. Natl. Acad. Sci. U. S. A., vol. 99, pp. 13148-13153.",
"Robertson, G.T., Reisenauer, A., Wright, R., Jensen, R.B., Jensen, A., Shapiro, L., Roop, R.M., (2000) J. Bacteriol., vol. 182, pp. 3482-3489.",
"Wright, R., Stephens, C., Shapiro, L., (1997) J. Bacteriol., vol. 179, pp. 5869-5877."
],
"prototype": "HinfI",
"organism": "Brucella abortus strain 2308",
"methylation_site": "2(6)"
},
"ecord3": {
"target_site": "GAANNNNNNNRTTC",
"name": "EcoRD3",
"suppliers": [],
"source": "T.A. Bickle",
"references": [
"Gubler, M., Braguglia, D., Meyer, J., Piekarowicz, A., Bickle, T.A., (1992) EMBO J., vol. 11, pp. 233-240."
],
"prototype": "",
"organism": "Artificial construct",
"methylation_site": "?(6)"
},
"ecord2": {
"target_site": "GAANNNNNNRTTC",
"name": "EcoRD2",
"suppliers": [],
"source": "T.A. Bickle",
"references": [
"Gubler, M., Braguglia, D., Meyer, J., Piekarowicz, A., Bickle, T.A., (1992) EMBO J., vol. 11, pp. 233-240."
],
"prototype": "",
"organism": "Artificial construct",
"methylation_site": "?(6)"
},
"uba1139i": {
"target_site": "CGATCG",
"name": "Uba1139I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Unidentified bacterium RFL1139",
"methylation_site": ""
},
"hsp92i": {
"target_site": "GR^CGYC",
"name": "Hsp92I",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "R. Williams",
"references": [
"Allen, J., Unpublished observations."
],
"prototype": "AcyI",
"organism": "Haemophilus species 92",
"methylation_site": ""
},
"bsub519i": {
"target_site": "GGATCC",
"name": "BsuB519I",
"suppliers": [],
"source": "Y.I. Buryanov",
"references": [
"Bogdarina, I.G., Nadirova, I.M., Buryanov, Y.I., (1986) Mikrobiologiia, vol. 55, pp. 699-700."
],
"prototype": "BamHI",
"organism": "Bacillus subtilis B519",
"methylation_site": ""
},
"hpyf34i": {
"target_site": "CTNAG",
"name": "HpyF34I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL34",
"methylation_site": ""
},
"m.phi3ti": {
"target_site": "GGCC,GCNGC",
"name": "M.Phi3TI",
"suppliers": [],
"source": "T.A. Trautner",
"references": [
"Gunthert, U., Trautner, T.A., (1984) Curr. Top. Microbiol. Immunol., vol. 108, pp. 11-22.",
"Noyer-Weidner, M., German Patent Office, 1989.",
"Noyer-Weidner, M., Jentsch, S., Kupsch, J., Bergbauer, M., Trautner, T.A., (1985) Gene, vol. 35, pp. 143-150.",
"Noyer-Weidner, M., Pawlek, B., Jentsch, S., Gunthert, U., Trautner, T.A., (1981) J. Virol., vol. 38, pp. 1077-1080."
],
"prototype": "HaeIII",
"organism": "Bacillus subtilis phage Phi3T",
"methylation_site": "3(5), 2(5)"
},
"uba1097i": {
"target_site": "GGCC",
"name": "Uba1097I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1097",
"methylation_site": ""
},
"bsaoi": {
"target_site": "CGRY^CG",
"name": "BsaOI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z.F., (1991) Chinese Sci. Bull., vol. 36, pp. 1231-1232."
],
"prototype": "McrI",
"organism": "Bacillus stearothermophilus O-122",
"methylation_site": ""
},
"drii": {
"target_site": "GACNNN^NNGTC",
"name": "DriI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Nayakshina, T.N., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Eam1105I",
"organism": "Deinococcus radiodurans EA",
"methylation_site": ""
},
"bstz1ii": {
"target_site": "AAGCTT",
"name": "BstZ1II",
"suppliers": [],
"source": "NEB 944",
"references": [
"Mruk, I., Kaczorowski, T., (2007) Appl. Environ. Microbiol., vol. 73, pp. 4286-4293.",
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Bacillus stearothermophilus 14P",
"methylation_site": ""
},
"atuiami": {
"target_site": "?",
"name": "AtuIAMI",
"suppliers": [],
"source": "IAM B-26-1",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "",
"organism": "Agrobacterium tumefaciens",
"methylation_site": ""
},
"bce170i": {
"target_site": "CTGCAG",
"name": "Bce170I",
"suppliers": [],
"source": "T. Ando",
"references": [
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "PstI",
"organism": "Bacillus cereus",
"methylation_site": ""
},
"bspovi": {
"target_site": "GACNNN^NNGTC",
"name": "BspOVI",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Chernov, A.V., Lepikhov, K.A., Zheleznaya, L.A., Matvienko, N.I., (1996) Biokhimiia, vol. 61, pp. 1837-1847."
],
"prototype": "Eam1105I",
"organism": "Bacillus species OV",
"methylation_site": ""
},
"mari": {
"target_site": "AGCT",
"name": "MarI",
"suppliers": [],
"source": "K. Dybvig",
"references": [
"Voelker, L.L., Dybvig, K., (1996) J. Bacteriol., vol. 178, pp. 6078-6081."
],
"prototype": "AluI",
"organism": "Mycoplasma arthritidis 1581",
"methylation_site": ""
},
"uba1421i": {
"target_site": "GRGCYC",
"name": "Uba1421I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1421",
"methylation_site": ""
},
"cvei": {
"target_site": "?",
"name": "CveI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Cystobacter velatus Plv9",
"methylation_site": ""
},
"esp14i": {
"target_site": "GGYRCC",
"name": "Esp14I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL14",
"methylation_site": ""
},
"uba1212i": {
"target_site": "CTGCAG",
"name": "Uba1212I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1212",
"methylation_site": ""
},
"hpyci": {
"target_site": "GAT^ATC",
"name": "HpyCI",
"suppliers": [],
"source": "NCTC 11637",
"references": [
"Ivic, A., Jakeman, K.J., Penn, C.W., Brown, N.L., (1999) FEMS Microbiol. Lett., vol. 179, pp. 175-180."
],
"prototype": "EcoRV",
"organism": "Helicobacter pylori",
"methylation_site": ""
},
"bbr7i": {
"target_site": "GAAGAC(7/11)",
"name": "Bbr7I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BbvII",
"organism": "Bacillus brevis 7",
"methylation_site": ""
},
"bsomai": {
"target_site": "GTCTC(1/5)",
"name": "BsoMAI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Shinkarenko, N.M., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsmAI",
"organism": "Bacillus stearothermophilus MA",
"methylation_site": ""
},
"bst224i": {
"target_site": "CCWWGG",
"name": "Bst224I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "StyI",
"organism": "Bacillus stearothermophilus I224",
"methylation_site": ""
},
"bsp8i": {
"target_site": "CCSGG",
"name": "Bsp8I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "CauII",
"organism": "Bacillus species RFL8",
"methylation_site": ""
},
"msp16i": {
"target_site": "TGGCCA",
"name": "Msp16I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BalI",
"organism": "Micrococcus species 16",
"methylation_site": ""
},
"cfr23i": {
"target_site": "GGNCC",
"name": "Cfr23I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Lebionka, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL23",
"methylation_site": ""
},
"hpyf74ii": {
"target_site": "ACGT",
"name": "HpyF74II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MaeII",
"organism": "Helicobacter pylori RFL74",
"methylation_site": ""
},
"mcri": {
"target_site": "CGRY^CG",
"name": "McrI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Brensing-Kuppers, J., Reischl, U., Schmitz, G.S., Kaluza, K., Jarsch, M., Kessler, C., (1990) FEBS Lett., vol. 264, pp. 218-222.",
"Kaluza, K., Hoeltke, H.J., Jarsch, M., Schmitz-Agheguian, G., Kessler, C., European Patent Office, 1989."
],
"prototype": "",
"organism": "Micrococcus cryophilus",
"methylation_site": ""
},
"tthhb27i": {
"target_site": "CAARCA",
"name": "TthHB27I",
"suppliers": [],
"source": "T. Hoshino",
"references": [
"Henne, A. et al., Unpublished observations.",
"Nie, C., Xu, S.-Y., Unpublished observations."
],
"prototype": "Tth111II",
"organism": "Thermus thermophilus HB27",
"methylation_site": "?(6)"
},
"mthti": {
"target_site": "GGCC",
"name": "MthTI",
"suppliers": [],
"source": "DSM 3848",
"references": [
"Nolling, J., de Vos, W.M., (1992) J. Bacteriol., vol. 174, pp. 5719-5726.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009."
],
"prototype": "HaeIII",
"organism": "Methanobacterium thermoformicicum THF",
"methylation_site": "3(5)"
},
"pfl37i": {
"target_site": "CTGCAG",
"name": "Pfl37I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas fluorescens RFL37",
"methylation_site": ""
},
"aspdii": {
"target_site": "GGWCC",
"name": "AspDII",
"suppliers": [],
"source": "ATCC 27898",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaII",
"organism": "Anabaena species",
"methylation_site": ""
},
"bbv12i": {
"target_site": "GWGCW^C",
"name": "Bbv12I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Hachaturyan, A.A., Afrikyan, E.K., (1989) Biol. J. Armenia, vol. 42, pp. 969-972."
],
"prototype": "HgiAI",
"organism": "Bacillus brevis 12",
"methylation_site": ""
},
"bsp47i": {
"target_site": "CCGG",
"name": "Bsp47I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Bacillus species RFL47",
"methylation_site": ""
},
"hpy401i": {
"target_site": "GTAC",
"name": "Hpy401I",
"suppliers": [],
"source": "N.R. Salama",
"references": [
"Humbert, O., Salama, N.R., (2008) Nucleic Acids Res., vol. 36, pp. 6893-6906.",
"Salama, N.R., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori 40-1",
"methylation_site": ""
},
"alui": {
"target_site": "AG^CT",
"name": "AluI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 21606",
"references": [
"Kramarov, V.M., Smolyaninov, V.V., (1981) Biokhimiia, vol. 46, pp. 1526-1529.",
"Labeots, L.A., (1993) Diss. Abstr., vol. 53, pp. 2842.",
"Roberts, R.J., Myers, P.A., Morrison, A., Murray, K., (1976) J. Mol. Biol., vol. 102, pp. 157-165.",
"Smith, M.D., Schmidt, B.J., Longo, M.C., Chatterjee, D.K., US Patent Office, 1994.",
"Ware, J., Zhang, B.-H., Slatko, B.S., Wilson, G.G., Unpublished observations.",
"Ware, J., Zhang, B.-H., Slatko, B.S., Wilson, G.G., Unpublished observations.",
"Yoon, H., Suh, H., Kim, K., Han, M.H., Yoo, O.J., (1985) Korean Biochem. J., vol. 18, pp. 88-93."
],
"prototype": "",
"organism": "Arthrobacter luteus",
"methylation_site": "3(5)"
},
"srl61di": {
"target_site": "TTTAAA",
"name": "Srl61DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "AhaIII",
"organism": "Selenomonas ruminantium 61",
"methylation_site": ""
},
"bpni": {
"target_site": "?",
"name": "BpnI",
"suppliers": [],
"source": "A.R. Reddy",
"references": [
"Jutur, P.P., Reddy, A.R., (2004) Biotechnol. Lett., vol. 26, pp. 929-932."
],
"prototype": "",
"organism": "Bacillus pantothenticus strain 1639",
"methylation_site": ""
},
"ecohk31i": {
"target_site": "Y^GGCCR",
"name": "EcoHK31I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.-F., Kam, K.-M., Shaw, P.-C., (1995) Nucleic Acids Res., vol. 23, pp. 103-108.",
"Lee, K.-F., Liaw, Y.-C., Shaw, P.-C., (1996) Biochem. J., vol. 314, pp. 321-326.",
"Lee, K.-F., Shaw, P.-C., Picone, S.J., Wilson, G.G., Lunnen, K.D., (1998) Biol. Chem., vol. 379, pp. 437-441."
],
"prototype": "CfrI",
"organism": "Escherichia coli HK31",
"methylation_site": "4(5)"
},
"bscri": {
"target_site": "RCCGGY",
"name": "BscRI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "Cfr10I",
"organism": "Bacillus species 4709",
"methylation_site": ""
},
"dpnii": {
"target_site": "^GATC",
"name": "DpnII",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "S. Lacks",
"references": [
"de la Campa, A.G., Kale, P., Springhorn, S.S., Lacks, S.A., (1987) J. Mol. Biol., vol. 196, pp. 457-469.",
"Lacks, S., Greenberg, B., (1975) J. Biol. Chem., vol. 250, pp. 4060-4066.",
"Lacks, S., Greenberg, B., (1977) J. Mol. Biol., vol. 114, pp. 153-168.",
"Lacks, S.A., Dunn, J.J., Greenberg, B., (1982) Cell, vol. 31, pp. 327-336.",
"Lacks, S.A., Springhorn, S.S., (1984) J. Bacteriol., vol. 157, pp. 934-936."
],
"prototype": "MboI",
"organism": "Diplococcus pneumoniae",
"methylation_site": "2(6)"
},
"bstmci": {
"target_site": "CGRY^CG",
"name": "BstMCI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "McrI",
"organism": "Bacillus stearothermophilus MC",
"methylation_site": ""
},
"hpych4i": {
"target_site": "CATG^",
"name": "HpyCH4I",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "NlaIII",
"organism": "Helicobacter pylori CH4",
"methylation_site": ""
},
"hpyf14iii": {
"target_site": "TCGA",
"name": "HpyF14III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL14",
"methylation_site": ""
},
"uba1229i": {
"target_site": "CCGCGG",
"name": "Uba1229I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1229",
"methylation_site": ""
},
"ccop13iii": {
"target_site": "CAYNNNNNRTG",
"name": "CcoP13III",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vital, J., Vitor, J.M.B., Unpublished observations."
],
"prototype": "CjuI",
"organism": "Campylobacter coli P13",
"methylation_site": ""
},
"bboi": {
"target_site": "?",
"name": "BboI",
"suppliers": [],
"source": "NEB 1047",
"references": [
"Ge, L., Unpublished observations."
],
"prototype": "",
"organism": "Brevibacillus borstelensis",
"methylation_site": ""
},
"pvu84i": {
"target_site": "CGATCG",
"name": "Pvu84I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Proteus vulgaris 84",
"methylation_site": ""
},
"aaqi": {
"target_site": "GTGCAC",
"name": "AaqI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "ApaLI",
"organism": "Alcaligenes aquamarinus 559",
"methylation_site": ""
},
"apui": {
"target_site": "GGNCC",
"name": "ApuI",
"suppliers": [],
"source": "M. Sargent",
"references": [
"Walker, J.N.B., Dean, P.G., Saunders, J.R., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Alteromonas putrefaciens",
"methylation_site": ""
},
"vneai": {
"target_site": "RGGNCCY",
"name": "VneAI",
"suppliers": [],
"source": "P. Wood",
"references": [
"Wood, P., Unpublished observations."
],
"prototype": "DraII",
"organism": "Vibrio nereis",
"methylation_site": ""
},
"bpu86i": {
"target_site": "GCCNNNNNGGC",
"name": "Bpu86I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BglI",
"organism": "Bacillus pumilis 86",
"methylation_site": ""
},
"hpyf71iii": {
"target_site": "GANTC",
"name": "HpyF71III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL71",
"methylation_site": ""
},
"psp6i": {
"target_site": "^CCWGG",
"name": "Psp6I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Tomilova, J.E., Kileva, E.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Pseudomonas species B6",
"methylation_site": ""
},
"uba1272i": {
"target_site": "GGWCC",
"name": "Uba1272I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1272",
"methylation_site": ""
},
"bsp57i": {
"target_site": "GATC",
"name": "Bsp57I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL57",
"methylation_site": ""
},
"uba1205i": {
"target_site": "GGATCC",
"name": "Uba1205I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1205",
"methylation_site": ""
},
"bsli": {
"target_site": "CCNNNNN^NNGG",
"name": "BslI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 606",
"references": [
"Cowan, D., Ward, J., Pelletier, J.J., Morgan, R., Unpublished observations.",
"Hsieh, P.-C., Xiao, J.-P., O'Loane, D., Xu, S.-Y., (2000) J. Bacteriol., vol. 182, pp. 949-955."
],
"prototype": "BsiYI",
"organism": "Bacillus species",
"methylation_site": "?(4)"
},
"pae18ki": {
"target_site": "A^GATCT",
"name": "Pae18kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravets, A.N., Pertsev, A.V., Tarutina, Z.Y., Krendelev, Y.D., Zakharova, M.V., Beletskaya, I.V., Solonin, A.S., (1998) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 14-17."
],
"prototype": "BglII",
"organism": "Pseudomonas aeruginosa 18k",
"methylation_site": ""
},
"asi372i": {
"target_site": "ATGCA^T",
"name": "Asi372I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Totmenina, O.D., Afonina, V.S., Polushkina, A.F., Repin, V.E., Russian Patent Office, 2007."
],
"prototype": "AvaIII",
"organism": "Arthrobacter species Mn372",
"methylation_site": ""
},
"hgigi": {
"target_site": "GR^CGYC",
"name": "HgiGI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Dusterhoft, A., (1990) Ph.D. Thesis, Justus-Liebig University, Geissen, W. Germany, pp. 1-160.",
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141."
],
"prototype": "AcyI",
"organism": "Herpetosiphon giganteus Hpa1",
"methylation_site": "?(5)"
},
"eco912i": {
"target_site": "CACNNNNNTGGC",
"name": "Eco912I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Kasarjian, J.K.A., Kodama, Y., Iida, M., Matsuda, K., Ryu, J., (2005) Nucleic Acids Res., vol. 33."
],
"prototype": "",
"organism": "Escherichia coli ECL 912",
"methylation_site": "?(6)"
},
"bbeai": {
"target_site": "GGCGCC",
"name": "BbeAI",
"suppliers": [],
"source": "ATCC 15698",
"references": [
"Khosaka, T., Unpublished observations."
],
"prototype": "NarI",
"organism": "Bifidobacterium breve S50",
"methylation_site": ""
},
"funii": {
"target_site": "G^AATTC",
"name": "FunII",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Waaijer, R.J.A., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Fischerella uniformis",
"methylation_site": ""
},
"uba72i": {
"target_site": "CTGCAG",
"name": "Uba72I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL72",
"methylation_site": ""
},
"eco64i": {
"target_site": "G^GYRCC",
"name": "Eco64I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Gilvonauskaite, R., Unpublished observations.",
"Naureckiene, S., Kazlauskiene, R., Maneliene, Z., Vaitkevicius, D., Butkus, V., Janulaitis, A., Unpublished observations.",
"Vaisvila, R., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Escherichia coli RFL64",
"methylation_site": ""
},
"uba89i": {
"target_site": "GTCGAC",
"name": "Uba89I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SalI",
"organism": "Unidentified bacterium RFL89",
"methylation_site": ""
},
"bst1107i": {
"target_site": "GTA^TAC",
"name": "Bst1107I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Trinkunaite, L., Kiuduliene, L., Maneliene, Z., Petrusyte, M., Butkus, V., Unpublished observations."
],
"prototype": "SnaI",
"organism": "Bacillus stearothermophilus RFL1107",
"methylation_site": ""
},
"mmeii": {
"target_site": "GATC",
"name": "MmeII",
"suppliers": [],
"source": "W.J. Brammar",
"references": [
"Bhatia, T., Morgan, R.D., Unpublished observations.",
"Boyd, A.C., Charles, I.G., Keyte, J.W., Brammar, W.J., (1986) Nucleic Acids Res., vol. 14, pp. 5255-5274.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "MboI",
"organism": "Methylophilus methylotrophus",
"methylation_site": ""
},
"hpyf13iv": {
"target_site": "GTAC",
"name": "HpyF13IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL13",
"methylation_site": ""
},
"pdii": {
"target_site": "GCC^GGC",
"name": "PdiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Mociskyte, S., Vaitkevicius, D., Maneliene, Z., Capskaja, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Pseudomonas diminuta Mck 33-321",
"methylation_site": ""
},
"mthfi": {
"target_site": "CTAG",
"name": "MthFI",
"suppliers": [],
"source": "DSM 3012",
"references": [
"Nolling, J., de Vos, W.M., (1992) Nucleic Acids Res., vol. 20, pp. 5047-5052."
],
"prototype": "MaeI",
"organism": "Methanobacterium thermoformicicum FTF",
"methylation_site": ""
},
"psp1009i": {
"target_site": "GCCNNNN^NGGC",
"name": "Psp1009I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Andreeva, I.S., Saranina, I.V., Pechurkina, N.I., Afonina, V.S., Repin, V.E., Russian Patent Office, 2008."
],
"prototype": "BglI",
"organism": "Paenibacillus species",
"methylation_site": ""
},
"bme46i": {
"target_site": "GGCC",
"name": "Bme46I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "HaeIII",
"organism": "Bacillus megaterium 46",
"methylation_site": ""
},
"nspliii": {
"target_site": "?",
"name": "NspLIII",
"suppliers": [],
"source": "C.P. Wolk",
"references": [
"Karreman, C., de Waard, A., Unpublished observations."
],
"prototype": "",
"organism": "Nostoc species UM-3",
"methylation_site": ""
},
"bth1202i": {
"target_site": "ATCGAT",
"name": "Bth1202I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "ClaI",
"organism": "Bacillus thuringiensis ssp. kumamotoensis",
"methylation_site": ""
},
"hinfiii": {
"target_site": "CGAAT",
"name": "HinfIII",
"suppliers": [],
"source": "ATCC 17947",
"references": [
"Fox, K.L., Dowideit, S.J., Erwin, A.L., Srikhanta, Y.N., Smith, A.L., Jennings, M.P., (2007) Nucleic Acids Res., vol. 35, pp. 5242-5252.",
"Kauc, L., Piekarowicz, A., (1978) Eur. J. Biochem., vol. 92, pp. 417-426.",
"Piekarowicz, A., Bickle, T.A., Shepherd, J.C.W., Ineichen, K., (1981) J. Mol. Biol., vol. 146, pp. 167-172.",
"Rao, D.N., Saha, S., Krishnamurthy, V., (2000) Prog. Nucleic Acid Res. Mol. Biol., vol. 64, pp. 1-63."
],
"prototype": "",
"organism": "Haemophilus influenzae Rf",
"methylation_site": "4(6)"
},
"eco646i": {
"target_site": "CCANNNNNNNCTTC",
"name": "Eco646I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Kasarjian, J.K.A., Iida, M., Ryu, J., (2003) Nucleic Acids Res., vol. 31."
],
"prototype": "",
"organism": "Escherichia coli EC646",
"methylation_site": "?(6)"
},
"salii": {
"target_site": "?",
"name": "SalII",
"suppliers": [],
"source": "ATCC 49789",
"references": [
"Arrand, J.R., Myers, P.A., Roberts, R.J., (1978) J. Mol. Biol., vol. 118, pp. 127-135."
],
"prototype": "",
"organism": "Streptomyces albus G",
"methylation_site": ""
},
"bsiai": {
"target_site": "GGCC",
"name": "BsiAI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus species",
"methylation_site": ""
},
"sdeosi": {
"target_site": "(11/13)GACNNNNRTGA(12/10)",
"name": "SdeOSI",
"suppliers": [],
"source": "DSM 15013",
"references": [
"Copeland, A. et al., Unpublished observations.",
"Wilson, G., Unpublished observations."
],
"prototype": "",
"organism": "Shewanella denitrificans OS217",
"methylation_site": "?(6)"
},
"lladchi": {
"target_site": "GATC",
"name": "LlaDCHI",
"suppliers": [],
"source": "S. Moineau",
"references": [
"Moineau, S., Walker, S.A., Vedamuthu, E.R., Vandenbergh, P.A., (1995) Appl. Environ. Microbiol., vol. 61, pp. 2193-2202."
],
"prototype": "MboI",
"organism": "Lactococcus lactis subsp. cremoris DCH-4",
"methylation_site": "2(6)"
},
"uba1402i": {
"target_site": "GGATCC",
"name": "Uba1402I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1402",
"methylation_site": ""
},
"m.h2i": {
"target_site": "GGCC",
"name": "M.H2I",
"suppliers": [],
"source": "T.A. Trautner",
"references": [
"Lange, C., Noyer-Weidner, M., Trautner, T.A., Weiner, M., Zahler, S.A., (1991) Gene, vol. 100, pp. 213-218."
],
"prototype": "HaeIII",
"organism": "Bacillus amyloliquefaciens phage H2",
"methylation_site": "?(5)"
},
"acai": {
"target_site": "TTCGAA",
"name": "AcaI",
"suppliers": [],
"source": "CCAP 1403/1",
"references": [
"Calleja, F., de Waard, A., Unpublished observations.",
"Hughes, S.G., Bruce, T., Murray, K., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Anabaena catenula",
"methylation_site": ""
},
"asusai": {
"target_site": "CCTNAGG",
"name": "AsuSAI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Degtyarev, S.Kh., (1998) Biol. Chem., vol. 379, pp. 573-574."
],
"prototype": "SauI",
"organism": "Actinobacillus suis SA",
"methylation_site": ""
},
"paii": {
"target_site": "GGCC",
"name": "PaiI",
"suppliers": [],
"source": "ATCC 12815",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "HaeIII",
"organism": "Pseudomonas alcaligenes",
"methylation_site": ""
},
"bst2i": {
"target_site": "CC^WGG",
"name": "Bst2I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus 2",
"methylation_site": ""
},
"eco249i": {
"target_site": "GRGCYC",
"name": "Eco249I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL249",
"methylation_site": ""
},
"cfrai": {
"target_site": "GCANNNNNNNNGTGG",
"name": "CfrAI",
"suppliers": [],
"source": "NCTC 9750",
"references": [
"Daniel, A.S., Fuller-Pace, F.V., Legge, D.M., Murray, N.E., (1988) J. Bacteriol., vol. 170, pp. 1775-1782.",
"Kannan, P., Cowan, G.M., Daniel, A.S., Gann, A.A.F., Murray, N.E., (1989) J. Mol. Biol., vol. 209, pp. 335-344."
],
"prototype": "",
"organism": "Citrobacter freundii",
"methylation_site": "?(6)"
},
"ese4i": {
"target_site": "GRGCYC",
"name": "Ese4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Enterobacter species RFL4",
"methylation_site": ""
},
"uba1372i": {
"target_site": "CCSGG",
"name": "Uba1372I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1372",
"methylation_site": ""
},
"chueii": {
"target_site": "GTYRAC",
"name": "ChuEII",
"suppliers": [],
"source": "ATCC 21108",
"references": [
"Endow, S.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HindII",
"organism": "Corynebacterium humiferum",
"methylation_site": ""
},
"msp23i": {
"target_site": "TCTAGA",
"name": "Msp23I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Andreeva, I.S., Puchkova, L.I., Unpublished observations."
],
"prototype": "XbaI",
"organism": "Micrococcus species 23",
"methylation_site": ""
},
"hal22i": {
"target_site": "GAATTC",
"name": "Hal22I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "EcoRI",
"organism": "Hafnia alvei 22",
"methylation_site": ""
},
"bsp106i": {
"target_site": "AT^CGAT",
"name": "Bsp106I",
"suppliers": [],
"source": "B. Zhou",
"references": [
"Nelson, M., McClelland, M., Unpublished observations.",
"Yan, P.F., Zhou, B., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus sphaericus 106",
"methylation_site": "5(6)"
},
"tfli": {
"target_site": "TCGA",
"name": "TflI",
"suppliers": [],
"source": "T. Oshima",
"references": [
"Sato, S., Nakazawa, K., Shinomiya, T., (1980) J. Biochem. (Tokyo), vol. 88, pp. 737-747."
],
"prototype": "TaqI",
"organism": "Thermus flavus AT62",
"methylation_site": ""
},
"mjaiv": {
"target_site": "GTNNAC",
"name": "MjaIV",
"suppliers": [],
"source": "DSM 2661",
"references": [
"Bult, C.J. et al., (1996) Science, vol. 273, pp. 1058-1073.",
"Morgan, R., Posfai, J., Patti, J., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Methanocaldococcus jannaschii",
"methylation_site": ""
},
"nmesi": {
"target_site": "AGTACT",
"name": "NmeSI",
"suppliers": [],
"source": "A. Van der Ende",
"references": [
"Bart, A., (1999) Ph.D. Thesis, Univ. Amsterdam, Netherlands (Ponsen & Looijen, BV, Wageningen), pp. 1-115.",
"Bart, A., Dankert, J., van der Ende, A., (1999) Mol. Microbiol., vol. 31, pp. 1275-1281.",
"Bart, A., Pannekoek, Y., Dankert, J., van der Ende, A., (2001) Infect. Immun., vol. 69, pp. 1816-1820."
],
"prototype": "ScaI",
"organism": "Neisseria meningitidis 800615",
"methylation_site": "5(4)"
},
"uba1343i": {
"target_site": "GGTCTC",
"name": "Uba1343I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Unidentified bacterium RFL1343",
"methylation_site": ""
},
"uba153ai": {
"target_site": "CAG^CTG",
"name": "Uba153AI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., (1995) Vestn. Akad. Med. Nauk SSSR, vol. 2, pp. 47-51."
],
"prototype": "PvuII",
"organism": "Unidentified bacterium 153A",
"methylation_site": ""
},
"sgr1841i": {
"target_site": "CTCGAG",
"name": "Sgr1841I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces griseoroseus 1841",
"methylation_site": ""
},
"ssvi": {
"target_site": "AGGCCT",
"name": "SsvI",
"suppliers": [],
"source": "ATCC 29083",
"references": [
"Mushtaq, R., Roberts, R.J., Unpublished observations."
],
"prototype": "StuI",
"organism": "Streptomyces sviceus",
"methylation_site": ""
},
"xbai": {
"target_site": "T^CTAGA",
"name": "XbaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 11672",
"references": [
"Patel, Y., Van Cott, E., Wilson, G.G., McClelland, M., (1990) Nucleic Acids Res., vol. 18, pp. 1603-1607.",
"Zain, B.S., Roberts, R.J., (1977) J. Mol. Biol., vol. 115, pp. 249-255."
],
"prototype": "",
"organism": "Xanthomonas badrii",
"methylation_site": "6(6)"
},
"btgzi": {
"target_site": "GCGATG(10/14)",
"name": "BtgZI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1384",
"references": [
"Morgan, R.D., Walsh, P., US Patent Office, 2006.",
"Pan, X.S., Morgan, R., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus thermoglucosidasius",
"methylation_site": "?(6)"
},
"srl5di": {
"target_site": "CTGCA^G",
"name": "Srl5DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Molnarova, V., Javorsky, P., (2002) Biologia (Bratisl), vol. 57, pp. 777-782.",
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "PstI",
"organism": "Selenomonas ruminantium 5",
"methylation_site": ""
},
"mjaii": {
"target_site": "GGNCC",
"name": "MjaII",
"suppliers": [],
"source": "DSM 2661",
"references": [
"Bult, C.J. et al., (1996) Science, vol. 273, pp. 1058-1073.",
"Zerler, B., Myers, P.A., Escalante, H., Roberts, R.J., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Methanocaldococcus jannaschii",
"methylation_site": "?(4)"
},
"mgoi": {
"target_site": "^GATC",
"name": "MgoI",
"suppliers": [],
"source": "A.K. Tyagi",
"references": [
"Shankar, S., Tyagi, A.K., (1993) Gene, vol. 131, pp. 153-154."
],
"prototype": "MboI",
"organism": "Mycobacterium gordonae",
"methylation_site": ""
},
"cfr30i": {
"target_site": "CCWGG",
"name": "Cfr30I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL30",
"methylation_site": ""
},
"dmoai": {
"target_site": "?",
"name": "DmoAI",
"suppliers": [],
"source": "W. Zillig",
"references": [
"McWilliam, P., Unpublished observations."
],
"prototype": "",
"organism": "Desulfococcus mobilus",
"methylation_site": ""
},
"hpyf71ii": {
"target_site": "GGNCC",
"name": "HpyF71II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Helicobacter pylori RFL71",
"methylation_site": ""
},
"bsu23i": {
"target_site": "T^CCGGA",
"name": "Bsu23I",
"suppliers": [],
"source": "V.M. Kramarov",
"references": [
"Kramarov, V.M., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Bacillus subtilis 23",
"methylation_site": ""
},
"uba1182i": {
"target_site": "GATC",
"name": "Uba1182I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1182",
"methylation_site": ""
},
"hgihiii": {
"target_site": "G^GWCC",
"name": "HgiHIII",
"suppliers": [],
"source": "J.H. Parish",
"references": [
"Whitehead, P.R., Jacobs, D., Brown, N.L., (1986) Nucleic Acids Res., vol. 14, pp. 7031-7045."
],
"prototype": "AvaII",
"organism": "Herpetosiphon giganteus HP1049",
"methylation_site": ""
},
"bpuni": {
"target_site": "GGGAC",
"name": "BpuNI",
"suppliers": [],
"source": "NEB 1316",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus pumilus 1703",
"methylation_site": ""
},
"bfai": {
"target_site": "C^TAG",
"name": "BfaI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1140",
"references": [
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"McLeod, B., Unpublished observations.",
"McLeod, E., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Reinecke, S.N., Morgan, R.D., (1991) Nucleic Acids Res., vol. 19, pp. 1152."
],
"prototype": "MaeI",
"organism": "Bacteroides fragilis",
"methylation_site": ""
},
"hpyf9ii": {
"target_site": "CTNAG",
"name": "HpyF9II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL9",
"methylation_site": ""
},
"aor51hi": {
"target_site": "AGC^GCT",
"name": "Aor51HI",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "Takara",
"references": [
"Sagawa, H., Takagi, M., Nomura, Y., Inagaki, K., Tano, T., Kishimato, N., Kotani, H., Nakajima, K., (1992) Nucleic Acids Res., vol. 20, pp. 365."
],
"prototype": "Eco47III",
"organism": "Acidiphilium organovorum 51H",
"methylation_site": ""
},
"ecori": {
"target_site": "G^AATTC",
"name": "EcoRI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "R.N. Yoshimori",
"references": [
"Albertsen, H.M., Le Paslier, D., Abderrahim, H., Dausset, J., Cann, H., Cohen, D., (1989) Nucleic Acids Res., vol. 17, pp. 808.",
"Dugaiczyk, A., Hedgpeth, J., Boyer, H.W., Goodman, H.M., (1974) Biochemistry, vol. 13, pp. 503-512.",
"Forrow, S., Lee, M., Souhami, R.L., Hartley, J.A., (1994) ACS Abstracts, vol. 208, pp. 97.",
"Greene, P.J., Betlach, M.C., Boyer, H.W., Goodman, H.M., (1974) Methods Mol. Biol., vol. 7, pp. 87-105.",
"Hedgpeth, J., Goodman, H.M., Boyer, H.W., (1972) Proc. Natl. Acad. Sci. U. S. A., vol. 69, pp. 3448-3452.",
"Pingoud, A., Alves, J., Fliess, A., Geiger, R., Rueter, T., Wolfes, H., (1987) Biol. Chem. Hoppe Seyler, vol. 368, pp. 1093.",
"Tanaka, M., Japanese Patent Office, 2002.",
"Winkler, F.K., (1994) J. Mol. Recognit., vol. 6, pp. 9."
],
"prototype": "",
"organism": "Escherichia coli RY13",
"methylation_site": "3(6)"
},
"bdisi": {
"target_site": "C^TRYAG",
"name": "BdiSI",
"suppliers": [],
"source": "T. Kanematsu",
"references": [
"Murakami, M., Ozawa, O., Kanematsu, T., Yamada, Y., (1990) Agric. Biol. Chem., vol. 54, pp. 275-277."
],
"prototype": "SfeI",
"organism": "Bacteroides distasonis",
"methylation_site": ""
},
"pfl19i": {
"target_site": "GGWCC",
"name": "Pfl19I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Pseudomonas fluorescens RFL19",
"methylation_site": ""
},
"hpy188i": {
"target_site": "TCN^GA",
"name": "Hpy188I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Stickel, S., Roberts, R.J., Blaser, M.J., Morgan, R.D., (2000) J. Biol. Chem., vol. 275, pp. 17086-17093."
],
"prototype": "",
"organism": "Helicobacter pylori J188",
"methylation_site": "5(6)"
},
"psp31i": {
"target_site": "GRGCYC",
"name": "Psp31I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Pseudomonas species RFL31",
"methylation_site": ""
},
"bsp4009i": {
"target_site": "G^GATCC",
"name": "Bsp4009I",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Korolev, S.V., Sokolov, N.N., Rina, M., Eldarov, M.A., Omelyanyuk, N.M., Markaki, M., Skryabin, K.G., Bouriotis, V., (1998) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 32-35."
],
"prototype": "BamHI",
"organism": "Bacillus species 4009",
"methylation_site": ""
},
"apai": {
"target_site": "GGGCC^C",
"name": "ApaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "NCIB 7215",
"references": [
"Gunthert, U., Trautner, T.A., (1984) Curr. Top. Microbiol. Immunol., vol. 108, pp. 11-22.",
"Kong, H., Unpublished observations.",
"Kong, H., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Seurinck, J., Van de Voorde, A., Van Montagu, M., (1983) Nucleic Acids Res., vol. 11, pp. 4409-4415.",
"Trautner, T.A., Unpublished observations.",
"Yamada, Y., Murakami, M., (1985) Agric. Biol. Chem., vol. 49, pp. 3627-3629."
],
"prototype": "",
"organism": "Acetobacter pasteurianus sub. pasteurianus",
"methylation_site": "4(5)"
},
"acoi": {
"target_site": "Y^GGCCR",
"name": "AcoI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Belichenko, O.A., Doroganov, A.O., Tomilova, J.E., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Acinetobacter calcoaceticus",
"methylation_site": ""
},
"sepi": {
"target_site": "ATGCA^T",
"name": "SepI",
"suppliers": [],
"source": "CAMB 2635",
"references": [
"Arif, A., Mushtaq, R., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Staphylococcus epidermidis",
"methylation_site": ""
},
"eco252i": {
"target_site": "GAATTC",
"name": "Eco252I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Escherichia coli RFL252",
"methylation_site": ""
},
"apadi": {
"target_site": "?",
"name": "ApaDI",
"suppliers": [],
"source": "J. Grones",
"references": [
"Grones, J., Turna, J., (1991) Biologia (Bratisl), vol. 46, pp. 1103-1108."
],
"prototype": "",
"organism": "Acetobacter pasteurianus D",
"methylation_site": ""
},
"bliri": {
"target_site": "AT^CGAT",
"name": "BliRI",
"suppliers": [],
"source": "ATCC 6634",
"references": [
"Mushtaq, R., Naeem, S., Riazuddin, S., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus licheniformis",
"methylation_site": ""
},
"bssfi": {
"target_site": "GCNGC",
"name": "BssFI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "Fnu4HI",
"organism": "Bacillus stearothermophilus F6",
"methylation_site": ""
},
"tsp219i": {
"target_site": "GCCNNNNNGGC",
"name": "Tsp219I",
"suppliers": [],
"source": "ITI 219",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BglI",
"organism": "Thermus species",
"methylation_site": ""
},
"bst28i": {
"target_site": "AT^CGAT",
"name": "Bst28I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Hachaturyan, A.A., Afrikyan, E.K., (1989) Biol. J. Armenia, vol. 42, pp. 969-972."
],
"prototype": "ClaI",
"organism": "Bacillus stearothermophilus 28",
"methylation_site": ""
},
"hpyf15ii": {
"target_site": "TCNGA",
"name": "HpyF15II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL15",
"methylation_site": ""
},
"uba38i": {
"target_site": "GGATCC",
"name": "Uba38I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL38",
"methylation_site": ""
},
"sflhk11087i": {
"target_site": "CCSGG",
"name": "SflHK11087I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "CauII",
"organism": "Shigella flexneri 11087",
"methylation_site": ""
},
"aosi": {
"target_site": "TGC^GCA",
"name": "AosI",
"suppliers": [],
"source": "CCAP 1403/11",
"references": [
"de Waard, A., van Beveren, C.P., Duyvesteyn, M., van Ormondt, H., (1979) FEBS Lett., vol. 101, pp. 71-76."
],
"prototype": "MstI",
"organism": "Anabaena oscillarioides",
"methylation_site": ""
},
"hgis21i": {
"target_site": "CCSGG",
"name": "HgiS21I",
"suppliers": [],
"source": "H. Foster",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "CauII",
"organism": "Herpetosiphon giganteus S21",
"methylation_site": ""
},
"ssp4800i": {
"target_site": "T^GTACA",
"name": "Ssp4800I",
"suppliers": [],
"source": "DSM 6181",
"references": [
"Kaluza, K., Frey, B., Jarsch, M., European Patent Office, 1995."
],
"prototype": "Bsp1407I",
"organism": "Streptomyces species BMTU 4800",
"methylation_site": ""
},
"animi": {
"target_site": "GCCGGC",
"name": "AniMI",
"suppliers": [],
"source": "ATCC 15236",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Arthrobacter nicotianae",
"methylation_site": ""
},
"bsp59i": {
"target_site": "GATC",
"name": "Bsp59I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL59",
"methylation_site": ""
},
"adei": {
"target_site": "CACNNN^GTG",
"name": "AdeI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Zutautiene, D., Capskaja, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "DraIII",
"organism": "Alcaligenes denitrificans Ss 3-028",
"methylation_site": ""
},
"tsp49i": {
"target_site": "ACGT^",
"name": "Tsp49I",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Williams, R.A.D., (1996) Nucleic Acids Res., vol. 24, pp. 1799-1801."
],
"prototype": "MaeII",
"organism": "Thermus species",
"methylation_site": ""
},
"uba1394i": {
"target_site": "ATCGAT",
"name": "Uba1394I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1394",
"methylation_site": ""
},
"pcisi": {
"target_site": "GCTCTTC(1/4)",
"name": "PciSI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SapI",
"organism": "Planococcus citreus S",
"methylation_site": ""
},
"eco1427i": {
"target_site": "GGANNNNNNNNATGC",
"name": "Eco1427I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco377I",
"organism": "Escherichia coli EC1427",
"methylation_site": ""
},
"pme35i": {
"target_site": "CCGG",
"name": "Pme35I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Pseudomonas medicaginis RFL35",
"methylation_site": ""
},
"eco13ki": {
"target_site": "^CCNGG",
"name": "Eco13kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Denjmukhametov, M.M., Zakharova, M.V., Kravets, A.N., Pertsev, A.V., Sineva, E.V., Repik, A.V., Beletskaya, I.V., Gromova, E.S., Solonin, A.S., (1997) Mol. Biol. (Mosk), vol. 31, pp. 831-838."
],
"prototype": "ScrFI",
"organism": "Escherichia coli 13k",
"methylation_site": ""
},
"nspiii": {
"target_site": "C^YCGRG",
"name": "NspIII",
"suppliers": [],
"source": "ATCC 29411",
"references": [
"Reaston, J., Duyvesteyn, M.G.C., de Waard, A., (1982) Gene, vol. 20, pp. 103-110.",
"Ueno, H., Ueno, T., Oshima, A., Kato, I., Japanese Patent Office, 1997."
],
"prototype": "AvaI",
"organism": "Nostoc species C",
"methylation_site": "?(4)"
},
"vpak11di": {
"target_site": "GGWCC",
"name": "VpaK11DI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus TVP1681",
"methylation_site": ""
},
"bsrsi": {
"target_site": "ACTGG(1/-1)",
"name": "BsrSI",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "BsrI",
"organism": "Bacillus stearothermophilus CPW19",
"methylation_site": ""
},
"hpyf31ii": {
"target_site": "GTSAC",
"name": "HpyF31II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL31",
"methylation_site": ""
},
"bshmi": {
"target_site": "CCGG",
"name": "BshMI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Bacillus sphaericus DC3",
"methylation_site": ""
},
"kspai": {
"target_site": "GTT^AAC",
"name": "KspAI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Vaitkevicius, D., Maneliene, Z., Norgeliene, D., Valickiene, D., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "HpaI",
"organism": "Kurthia species N88",
"methylation_site": ""
},
"hpyhpk5i": {
"target_site": "CTNAG",
"name": "HpyHPK5I",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori HPK5",
"methylation_site": ""
},
"bchi": {
"target_site": "GCAGC",
"name": "BchI",
"suppliers": [],
"source": "NEB 955",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "BbvI",
"organism": "Bacillus chitinosporus",
"methylation_site": "?(5)"
},
"ubaf11i": {
"target_site": "TCGTA",
"name": "UbaF11I",
"suppliers": [],
"source": "Fermentas G327",
"references": [
"Vitkute, J., Lauciuniene, N., Riauba, L., Capskaja, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Unidentified bacterium F11",
"methylation_site": ""
},
"m.pgii": {
"target_site": "GATC",
"name": "M.PgiI",
"suppliers": [],
"source": "A. Progulske-Fox",
"references": [
"Banas, J.A., Ferretti, J.J., Progulske-Fox, A., (1991) Nucleic Acids Res., vol. 19, pp. 4189-4192."
],
"prototype": "MboI",
"organism": "Porphyromonas gingivalis",
"methylation_site": "2(6)"
},
"abei": {
"target_site": "CCTCAGC(-5/-2)",
"name": "AbeI",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Maneliene, Z., Janulaitis, A., (1998) Nucleic Acids Res., vol. 26, pp. 4917-4918."
],
"prototype": "BbvCI",
"organism": "Azotobacter beijerinckii Slo 54-028",
"methylation_site": ""
},
"uba1389i": {
"target_site": "CCSGG",
"name": "Uba1389I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1389",
"methylation_site": ""
},
"cvisiii": {
"target_site": "TCGA",
"name": "CviSIII",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Nelson, M., Que, Q., Van Etten, J.L., Unpublished observations.",
"Que, Q., Zhang, Y., Nelson, M., Ropp, S., Burbank, D.E., Van Etten, J.L., (1997) Gene, vol. 190, pp. 237-244."
],
"prototype": "TaqI",
"organism": "Chlorella strain NC64A (SC-1A)",
"methylation_site": "4(6)"
},
"bsp148i": {
"target_site": "TTCGAA",
"name": "Bsp148I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species RFL148",
"methylation_site": ""
},
"cpei": {
"target_site": "TGATCA",
"name": "CpeI",
"suppliers": [],
"source": "ATCC 19080",
"references": [
"Fisherman, J., Gingeras, T.R., Roberts, R.J., Unpublished observations."
],
"prototype": "BclI",
"organism": "Corynebacterium petrophilum",
"methylation_site": ""
},
"asp52i": {
"target_site": "AAGCTT",
"name": "Asp52I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Alcaligenes species 52",
"methylation_site": ""
},
"bsogii": {
"target_site": "?",
"name": "BsoGII",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus Lipari 29",
"methylation_site": ""
},
"hpyf64v": {
"target_site": "CTNAG",
"name": "HpyF64V",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL64",
"methylation_site": ""
},
"hpy8iii": {
"target_site": "GWGCWC",
"name": "Hpy8III",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Trinkunaite, L., Kiuduliene, E., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Helicobacter pylori A 8-5",
"methylation_site": ""
},
"uba1176i": {
"target_site": "GGCC",
"name": "Uba1176I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1176",
"methylation_site": ""
},
"bsp211i": {
"target_site": "GG^CC",
"name": "Bsp211I",
"suppliers": [],
"source": "P.F. Yan",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus 211",
"methylation_site": ""
},
"hpyf64i": {
"target_site": "TCGA",
"name": "HpyF64I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL64",
"methylation_site": ""
},
"umi5i": {
"target_site": "CYCGRG",
"name": "Umi5I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Unidentified bacterium 5T",
"methylation_site": ""
},
"avaii": {
"target_site": "G^GWCC",
"name": "AvaII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 27893",
"references": [
"Fuchs, C., Rosenvold, E.C., Honigman, A., Szybalski, W., (1978) Gene, vol. 4, pp. 1-23.",
"Hughes, S.G., Murray, K., (1980) Biochem. J., vol. 185, pp. 65-75.",
"Kaneko, T. et al., (2001) DNA Res., vol. 8, pp. 205-213.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Lunnen, K.D., Wilson, G.G., Kroeger, M., Unpublished observations.",
"Murray, K., Hughes, S.G., Brown, J.S., Bruce, S.A., (1976) Biochem. J., vol. 159, pp. 317-322.",
"Sutcliffe, J.G., Church, G.M., (1978) Nucleic Acids Res., vol. 5, pp. 2313-2319."
],
"prototype": "",
"organism": "Anabaena variabilis",
"methylation_site": "?(5)"
},
"cac8i": {
"target_site": "GCN^NGC",
"name": "Cac8I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "G. Reysett",
"references": [
"Azeddoug, H., Reysset, G., (1991) FEMS Microbiol. Lett., vol. 78, pp. 153-156.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Clostridium acetobutylicum ABKn8",
"methylation_site": "?(5)"
},
"bsp12ii": {
"target_site": "?",
"name": "Bsp12II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species RFL12",
"methylation_site": ""
},
"bsu1192i": {
"target_site": "CCGG",
"name": "Bsu1192I",
"suppliers": [],
"source": "IAM 1192",
"references": [
"Qiang, B.-Q., Schildkraut, I., Unpublished observations.",
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "HpaII",
"organism": "Bacillus subtilis",
"methylation_site": ""
},
"tlii": {
"target_site": "C^TCGAG",
"name": "TliI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 521",
"references": [
"Krotee, S., Ganatra, M., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "XhoI",
"organism": "Thermococcus litoralis",
"methylation_site": ""
},
"mkii": {
"target_site": "AAGCTT",
"name": "MkiI",
"suppliers": [],
"source": "ATCC 23331",
"references": [
"Jiang, B.D., Myers, P., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Moraxella kingae",
"methylation_site": ""
},
"srl32dii": {
"target_site": "G^AATTC",
"name": "Srl32DII",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "EcoRI",
"organism": "Selenomonas ruminantium 32",
"methylation_site": ""
},
"nmeiii": {
"target_site": "?",
"name": "NmeIII",
"suppliers": [],
"source": "R. Sparling",
"references": [
"Sparling, R., Bhatti, A.R., (1983) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 83, pp. 214."
],
"prototype": "",
"organism": "Neisseria meningitidis M1011",
"methylation_site": ""
},
"bsp90i": {
"target_site": "TTCGAA",
"name": "Bsp90I",
"suppliers": [],
"source": "ITI 90",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"trui": {
"target_site": "GGWCC",
"name": "TruI",
"suppliers": [],
"source": "BKM B-1258",
"references": [
"Bernal, W.M., Raven, N.D.H., Williams, R.A.D., (1986) Proceedings 14th Int. Congress Microbiol., vol. 0, pp. 204."
],
"prototype": "AvaII",
"organism": "Thermus ruber strain 21",
"methylation_site": ""
},
"teli": {
"target_site": "GACN^NNGTC",
"name": "TelI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Bax, E., Unpublished observations."
],
"prototype": "Tth111I",
"organism": "Tolypothrix elabens",
"methylation_site": ""
},
"bmti": {
"target_site": "GCTAG^C",
"name": "BmtI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "NEB 1776",
"references": [
"Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K., (2003) Biotekhnologiya, vol. 1, pp. 11-15.",
"Zhu, Z., Unpublished observations."
],
"prototype": "NheI",
"organism": "Bacillus megaterium S2",
"methylation_site": "?(5)"
},
"bfli": {
"target_site": "CCNNNNN^NNGG",
"name": "BflI",
"suppliers": [],
"source": "P. Sharma",
"references": [
"D'Souza, D.R., Morgan, R.D., Parashar, V., Capalash, N., Sharma, P., (2004) World J. Microbiol. Biotechnol., vol. 20, pp. 593-598."
],
"prototype": "BsiYI",
"organism": "Bacillus flavothermus",
"methylation_site": ""
},
"hpyf72ii": {
"target_site": "CTNAG",
"name": "HpyF72II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL72",
"methylation_site": ""
},
"acuii": {
"target_site": "CCWGG",
"name": "AcuII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Kileva, E.V., Dedkov, V.S., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Acinetobacter calcoaceticus SRW4",
"methylation_site": ""
},
"avai": {
"target_site": "C^YCGRG",
"name": "AvaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 27893",
"references": [
"Hughes, S.G., Murray, K., (1980) Biochem. J., vol. 185, pp. 65-75.",
"Kaneko, T. et al., (2001) DNA Res., vol. 8, pp. 205-213.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Murray, K., Hughes, S.G., Brown, J.S., Bruce, S.A., (1976) Biochem. J., vol. 159, pp. 317-322.",
"Ruan, H., Lunnen, K.D., Scott, M.E., Moran, L.S., Slatko, B.E., Pelletier, J.J., Hess, E.J., Benner, J., Wilson, G.G., Xu, S.-Y., (1996) Mol. Gen. Genet., vol. 252, pp. 695-699.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "",
"organism": "Anabaena variabilis",
"methylation_site": "?(4)"
},
"baci": {
"target_site": "CCGCGG",
"name": "BacI",
"suppliers": [],
"source": "ATCC 27009",
"references": [
"Mayer, H., Schutte, H., Unpublished observations.",
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "SacII",
"organism": "Bacillus acidocaldarius",
"methylation_site": ""
},
"tru201i": {
"target_site": "R^GATCY",
"name": "Tru201I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations.",
"Tsaplina, I.A., Bogdanova, T.I., Rechkunova, N.I., Degtyarev, S.K., Soviet Patent Office, 1992."
],
"prototype": "XhoII",
"organism": "Thermus ruber 201",
"methylation_site": ""
},
"bscfi": {
"target_site": "^GATC",
"name": "BscFI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species JY391",
"methylation_site": ""
},
"gsti": {
"target_site": "G^GATCC",
"name": "GstI",
"suppliers": [],
"source": "ATCC 7953",
"references": [
"Skowron, P.M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Geobacillus stearothermophilus",
"methylation_site": ""
},
"bazi": {
"target_site": "ATCGAT",
"name": "BazI",
"suppliers": [],
"source": "ATCC 29788",
"references": [
"Waaijer, R.J.A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus azotoformans",
"methylation_site": ""
},
"ecodi": {
"target_site": "TTANNNNNNNGTCY",
"name": "EcoDI",
"suppliers": [],
"source": "N.E. Murray",
"references": [
"Nagaraja, V., Stieger, M., Nager, C., Hadi, S.M., Bickle, T.A., (1985) Nucleic Acids Res., vol. 13, pp. 389-399."
],
"prototype": "",
"organism": "Escherichia coli E166",
"methylation_site": "?(6)"
},
"hsp92ii": {
"target_site": "CATG^",
"name": "Hsp92II",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "R. Williams",
"references": [
"Allen, J., Unpublished observations."
],
"prototype": "NlaIII",
"organism": "Haemophilus species 92",
"methylation_site": ""
},
"hincii": {
"target_site": "GTY^RAC",
"name": "HincII",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 49699",
"references": [
"Benner, J.S., Rees, P.A., European Patent Office, 1995.",
"Ii, J.S.B., Rees, P.A., Japanese Patent Office, 1998.",
"Ito, H., Sadaoka, A., Japanese Patent Office, 1991.",
"Ito, H., Sadaoka, A., Kotani, H., Hiraoka, N., Nakamura, T., (1990) Nucleic Acids Res., vol. 18, pp. 3903-3911.",
"Landy, A., Ruedisueli, E., Robinson, L., Foeller, C., Ross, W., (1974) Biochemistry, vol. 13, pp. 2134-2142.",
"Waite-Rees, P.A., Nwankwo, D., Moran, L., Slatko, B.S., Wilson, G.G., Unpublished observations."
],
"prototype": "HindII",
"organism": "Haemophilus influenzae Rc",
"methylation_site": "5(6)"
},
"tnei": {
"target_site": "GCWGC",
"name": "TneI",
"suppliers": [],
"source": "T. Lowe",
"references": [
"Copeland, A. et al., Unpublished observations."
],
"prototype": "TseI",
"organism": "Thermoproteus neutrophilus V24Sta",
"methylation_site": "?(5)"
},
"bsthhi": {
"target_site": "GCG^C",
"name": "BstHHI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shapovalova, M.A., Abdurashitov, M.A., Tumanova, I.Y., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Bacillus stearothermophilus HH",
"methylation_site": ""
},
"sfci": {
"target_site": "C^TRYAG",
"name": "SfcI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 674",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., Meloni, A., Unpublished observations.",
"Zhu, Z., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Streptococcus faecium",
"methylation_site": ""
},
"bspqi": {
"target_site": "GCTCTTC(1/4)",
"name": "BspQI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1214",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations.",
"Xu, S.-Y., Unpublished observations.",
"Zhang, P.H., Too, P.H.M., Samuelson, J.C., Chan, S.H., Vincze, T., Doucette, S., Backstrom, S., Potamousis, K.D., Schramm, T.M., Forrest, D., Schwartz, D.C., Xu, S.Y., (2010) Protein Expr. Purif., vol. 69, pp. 226-234.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chan, S.-h., International Patent Office, 2009.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chang, S.-H., International Patent Office, 2009.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "SapI",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"lgui": {
"target_site": "GCTCTTC(1/4)",
"name": "LguI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G307",
"references": [
"Vitkute, J., Lapcinskaja, S., Maneliene, Z., Capskaja, L., Trinkunaite, L., Janulaitis, A., Unpublished observations."
],
"prototype": "SapI",
"organism": "Lysobacter gummosus RFL1",
"methylation_site": ""
},
"eclji": {
"target_site": "CGATCG",
"name": "EclJI",
"suppliers": [],
"source": "CAMB 2542",
"references": [
"Maqbool, T., Sohail, A., Choudhry, S., Riazuddin, S., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Enterobacter cloacae J",
"methylation_site": ""
},
"ngoci": {
"target_site": "RGCGCY",
"name": "NgoCI",
"suppliers": [],
"source": "CDC 66",
"references": [
"Clanton, D.J., Riggsby, W.S., Miller, R.V., (1979) J. Bacteriol., vol. 137, pp. 1299-1307."
],
"prototype": "HaeII",
"organism": "Neisseria gonorrhoeae",
"methylation_site": ""
},
"fnuei": {
"target_site": "^GATC",
"name": "FnuEI",
"suppliers": [],
"source": "M. Smith",
"references": [
"Lui, A.C.P., McBride, B.C., Vovis, G.F., Smith, M., (1979) Nucleic Acids Res., vol. 6, pp. 1-15."
],
"prototype": "MboI",
"organism": "Fusobacterium nucleatum E",
"methylation_site": ""
},
"uth554i": {
"target_site": "GGWCC",
"name": "Uth554I",
"suppliers": [],
"source": "FZ 554",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium",
"methylation_site": ""
},
"hingui": {
"target_site": "GCGC",
"name": "HinGUI",
"suppliers": [],
"source": "J. Chirikjian",
"references": [
"Chirikjian, J.G., George, A., Smith, L.A., (1978) Fed. Proc., vol. 37, pp. 1415.",
"Smith, L., Blakesley, R., Chirikjian, J., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Haemophilus influenzae GU",
"methylation_site": ""
},
"dpni": {
"target_site": "GA^TC",
"name": "DpnI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Stratagene (3/10)",
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"EURx Ltd. (1/11)"
],
"source": "S. Lacks",
"references": [
"Geier, G.E., Modrich, P., (1979) J. Biol. Chem., vol. 254, pp. 1408-1413.",
"Lacks, S., Greenberg, B., (1975) J. Biol. Chem., vol. 250, pp. 4060-4066.",
"Lacks, S., Greenberg, B., (1977) J. Mol. Biol., vol. 114, pp. 153-168."
],
"prototype": "",
"organism": "Diplococcus pneumoniae",
"methylation_site": ""
},
"psb9879i": {
"target_site": "GGCC",
"name": "Psb9879I",
"suppliers": [],
"source": "A. Murray",
"references": [
"Miller, J., Murray, A., Bhatia, T., Morgan, R.D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Polar sea bacterium R9879, C5SW4c3",
"methylation_site": ""
},
"bstmi": {
"target_site": "AGTACT",
"name": "BstMI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Bacillus stearothermophilus M571",
"methylation_site": ""
},
"alwni": {
"target_site": "CAGNNN^CTG",
"name": "AlwNI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 419",
"references": [
"Morgan, R.D., Dalton, M., Stote, R., (1987) Nucleic Acids Res., vol. 15, pp. 7201.",
"Zhu, Z., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Acinetobacter lwoffi",
"methylation_site": ""
},
"bsali": {
"target_site": "AGCT",
"name": "BsaLI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "AluI",
"organism": "Bacillus stearothermophilus L170",
"methylation_site": ""
},
"ecot104i": {
"target_site": "CCWWGG",
"name": "EcoT104I",
"suppliers": [],
"source": "N. Terakado",
"references": [
"Yoshida, Y., Mise, K., Unpublished observations."
],
"prototype": "StyI",
"organism": "Escherichia coli TB104",
"methylation_site": ""
},
"hin1i": {
"target_site": "GR^CGYC",
"name": "Hin1I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "AcyI",
"organism": "Haemophilus influenzae RFL1",
"methylation_site": ""
},
"bstkti": {
"target_site": "GAT^C",
"name": "BstKTI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Nadeev, A.N., Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus KT",
"methylation_site": ""
},
"bsoci": {
"target_site": "GDGCH^C",
"name": "BsoCI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations.",
"Wright, D.J., Unpublished observations."
],
"prototype": "SduI",
"organism": "Bacillus stearothermophilus AU891",
"methylation_site": ""
},
"saubmki": {
"target_site": "GCC^GGC",
"name": "SauBMKI",
"suppliers": [],
"source": "J. Timko",
"references": [
"Timko, J., Turna, J., Zelinka, J., (1987) Metabolism and Enzymology of Nucleic Acids, ed. Zelinka, J., Balan, J. (Publishing House of the Slovak Akademy of Science, Bratislava), vol. 6, pp. 107-118."
],
"prototype": "NaeI",
"organism": "Streptomyces aureofaciens strain BM-K",
"methylation_site": ""
},
"bschi": {
"target_site": "ACTGG",
"name": "BscHI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "BsrI",
"organism": "Bacillus species A",
"methylation_site": ""
},
"uba1246i": {
"target_site": "ATCGAT",
"name": "Uba1246I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1246",
"methylation_site": ""
},
"gspaii": {
"target_site": "TGCGCA",
"name": "GspAII",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "MstI",
"organism": "Gloeotricia species",
"methylation_site": ""
},
"csti": {
"target_site": "CTGCA^G",
"name": "CstI",
"suppliers": [],
"source": "C.S. Chen",
"references": [
"Jou, T.-W., Chen, C.-S., (1988) Bot. Bull. Acad. Sinica, vol. 29, pp. 201-208."
],
"prototype": "PstI",
"organism": "Clostridium sticklandii",
"methylation_site": ""
},
"bse21i": {
"target_site": "CC^TNAGG",
"name": "Bse21I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.Kh., Rechkunova, N.I., Repin, V.E., Kolyhalov, A.A., Netesov, S.V., (1990) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 1, pp. 138-139.",
"Repin, V.E., Polshina, S.V., Bozhko, N.A., Degtyarev, S.Kh., (1989) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 15, pp. 108-111."
],
"prototype": "SauI",
"organism": "Bacillus species 21",
"methylation_site": ""
},
"stysti": {
"target_site": "?",
"name": "StySTI",
"suppliers": [],
"source": "L.R. Bullas",
"references": [
"Campbell, A.J.B., Murray, N.E., Unpublished observations."
],
"prototype": "",
"organism": "Salmonella thompson UA-T11",
"methylation_site": ""
},
"zani": {
"target_site": "CC^WGG",
"name": "ZanI",
"suppliers": [],
"source": "O.J. Yoo",
"references": [
"Sun, D.K., Yoo, O.J., (1988) Korean Biochem. J., vol. 21, pp. 419-422."
],
"prototype": "EcoRII",
"organism": "Zymomonas anaerobia",
"methylation_site": ""
},
"cfr48i": {
"target_site": "GRGCYC",
"name": "Cfr48I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Citrobacter freundii RFL48",
"methylation_site": ""
},
"psc97i": {
"target_site": "?",
"name": "Psc97I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species 97",
"methylation_site": ""
},
"uba1138i": {
"target_site": "ATCGAT",
"name": "Uba1138I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1138",
"methylation_site": ""
},
"bspmi": {
"target_site": "ACCTGC(4/8)",
"name": "BspMI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 356",
"references": [
"Morgan, R., Hoffman, L., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Bacillus species M",
"methylation_site": "?(5)"
},
"hpyf35i": {
"target_site": "TCGA",
"name": "HpyF35I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL35",
"methylation_site": ""
},
"rspi": {
"target_site": "CGATCG",
"name": "RspI",
"suppliers": [],
"source": "R. Lascelles",
"references": [
"Bingham, A.H.A., Atkinson, T., Darbyshire, J., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Rhodopseudomonas sphaeroides",
"methylation_site": ""
},
"btgaii": {
"target_site": "GCATGC",
"name": "BtgAII",
"suppliers": [],
"source": "NEB 969",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "SphI",
"organism": "Brevibacterium thiogenitalis",
"methylation_site": ""
},
"bstv1i": {
"target_site": "GCAGC(8/12)",
"name": "BstV1I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BbvI",
"organism": "Bacillus stearothermophilus V1",
"methylation_site": ""
},
"bsumi": {
"target_site": "CTCGAG",
"name": "BsuMI",
"suppliers": [],
"source": "A. Danchin",
"references": [
"Guha, S., (1985) J. Bacteriol., vol. 163, pp. 573-579.",
"Jentsch, S., (1983) J. Bacteriol., vol. 156, pp. 800-808.",
"Kunst, F. et al., (1997) Nature, vol. 390, pp. 249-256.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Sadaie, Y., Unpublished observations.",
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "XhoI",
"organism": "Bacillus subtilis 168",
"methylation_site": "?(5)"
},
"uba1286i": {
"target_site": "ATCGAT",
"name": "Uba1286I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1286",
"methylation_site": ""
},
"eco125i": {
"target_site": "CTGAAG",
"name": "Eco125I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "Eco57I",
"organism": "Escherichia coli RFL125",
"methylation_site": ""
},
"acs1421i": {
"target_site": "GTCGAC",
"name": "Acs1421I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Hamafuku, H., Kotani, H., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "SalI",
"organism": "Acidobacterium capsulatum 1421",
"methylation_site": ""
},
"lpli": {
"target_site": "AT^CGAT",
"name": "LplI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Maneliene, Z.P., Butkus, V.V., Fitzner, A.B., Khoroshutina, E.B., Kalugin, A.A., Janulaitis, A., (1990) Bioorg. Khim., vol. 16, pp. 1040-1044."
],
"prototype": "ClaI",
"organism": "Lactobacillus plantarum",
"methylation_site": ""
},
"bsrai": {
"target_site": "G^GWCC",
"name": "BsrAI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations.",
"Wright, D.J., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus stearothermophilus CPW7",
"methylation_site": ""
},
"ngbi": {
"target_site": "CTGCAG",
"name": "NgbI",
"suppliers": [],
"source": "ATCC 21292",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "PstI",
"organism": "Nocardia globerula",
"methylation_site": ""
},
"nmuei": {
"target_site": "GATC",
"name": "NmuEI",
"suppliers": [],
"source": "ATCC 25996",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations."
],
"prototype": "DpnI",
"organism": "Neisseria mucosa",
"methylation_site": ""
},
"eco52i": {
"target_site": "C^GGCCG",
"name": "Eco52I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Butkus, V.V., Petrusyte, M.P., Janulaitis, A., (1985) Bioorg. Khim., vol. 11, pp. 987-988.",
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129."
],
"prototype": "XmaIII",
"organism": "Escherichia coli RFL52",
"methylation_site": ""
},
"aspmdi": {
"target_site": "^GATC",
"name": "AspMDI",
"suppliers": [],
"source": "H.X. Chiura",
"references": [
"Chiura, H.X., Kamiyama, T., Hirano, H., Futagami, M., Watahiki, M., Kobayashi, K., Simidu, U., Takagi, J., (1992) Nucleic Acids Res., vol. 20, pp. 1996."
],
"prototype": "MboI",
"organism": "Alcaligenes species MD1",
"methylation_site": ""
},
"vcho85i": {
"target_site": "GGNCC",
"name": "VchO85I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "AsuI",
"organism": "Vibrio cholerae 1903-83",
"methylation_site": ""
},
"cfr55i": {
"target_site": "YGGCCR",
"name": "Cfr55I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Citrobacter freundii RFL55",
"methylation_site": ""
},
"r1.ssudat1i": {
"target_site": "GATC",
"name": "R1.SsuDAT1I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Otani, Y., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 500-511."
],
"prototype": "MboI",
"organism": "Streptococcus suis DAT1",
"methylation_site": ""
},
"hin5i": {
"target_site": "CCGG",
"name": "Hin5I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "HpaII",
"organism": "Haemophilus influenzae RFL5",
"methylation_site": ""
},
"tsp560i": {
"target_site": "GGCC",
"name": "Tsp560I",
"suppliers": [],
"source": "ITI 51",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Thermus species",
"methylation_site": ""
},
"rma495i": {
"target_site": "CTAG",
"name": "Rma495I",
"suppliers": [],
"source": "ITI 495",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"uba1404i": {
"target_site": "CGCG",
"name": "Uba1404I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Unidentified bacterium RFL1404",
"methylation_site": ""
},
"hinjci": {
"target_site": "GTY^RAC",
"name": "HinJCI",
"suppliers": [],
"source": "A. Piekarowicz",
"references": [
"Piekarowicz, A., Stasiak, A., Stanczak, J., (1980) Acta Microbiol. Pol., vol. 29, pp. 151-156."
],
"prototype": "HindII",
"organism": "Haemophilus influenzae JC9",
"methylation_site": ""
},
"uba1451i": {
"target_site": "ATCGAT",
"name": "Uba1451I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1451",
"methylation_site": ""
},
"bstz9i": {
"target_site": "ACGCGT",
"name": "BstZ9I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "MluI",
"organism": "Bacillus stearothermophilus 34G",
"methylation_site": ""
},
"uba1154i": {
"target_site": "CTCGAG",
"name": "Uba1154I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1154",
"methylation_site": ""
},
"hpyf10vi": {
"target_site": "GCNNNNN^NNGC",
"name": "HpyF10VI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MwoI",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"uba1101i": {
"target_site": "GATC",
"name": "Uba1101I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1101",
"methylation_site": ""
},
"uba59i": {
"target_site": "GATC",
"name": "Uba59I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL59",
"methylation_site": ""
},
"bstbaii": {
"target_site": "CYCGRG",
"name": "BstBAII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Bacillus stearothermophilus BA",
"methylation_site": ""
},
"nmeii": {
"target_site": "?",
"name": "NmeII",
"suppliers": [],
"source": "R. Sparling",
"references": [
"Sparling, R., Bhatti, A.R., (1983) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 83, pp. 214."
],
"prototype": "",
"organism": "Neisseria meningitidis DRES-W34",
"methylation_site": ""
},
"uba1442i": {
"target_site": "CCNNGG",
"name": "Uba1442I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SecI",
"organism": "Unidentified bacterium RFL1442",
"methylation_site": ""
},
"m.crednmt1": {
"target_site": "?",
"name": "M.CreDnmt1",
"suppliers": [],
"source": "J.-D. Rochaix",
"references": [
"Kobayashi, I., (2001) Tanpakushitsu Kakusan Koso, vol. 46, pp. 2393-2399.",
"Nishiyama, R., Ito, M., Yamaguchi, Y., Koizumi, N., Sano, H., (2002) Plant Cell Physiol., vol. 43.",
"Nishiyama, R., Wada, Y., Mibu, M., Yamaguchi, Y., Shimogawara, K., Sano, H., (2004) Genetics, vol. 168, pp. 809-816."
],
"prototype": "",
"organism": "Chlamydomonas reinhardtii",
"methylation_site": "?(5)"
},
"uba1116i": {
"target_site": "CTGCAG",
"name": "Uba1116I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1116",
"methylation_site": ""
},
"blpi": {
"target_site": "GC^TNAGC",
"name": "BlpI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 819",
"references": [
"Chang, Z., Morgan, R., Unpublished observations.",
"Heiter, D., Lunnen, K., Wilson, G.G., US Patent Office, 2006.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., Unpublished observations."
],
"prototype": "EspI",
"organism": "Bacillus species lp",
"methylation_site": "?(5)"
},
"m.fvi3i": {
"target_site": "?",
"name": "M.Fvi3I",
"suppliers": [],
"source": "K. Kaur",
"references": [
"Willis, D.B., Goorha, R., Granoff, A., (1984) J. Virol., vol. 49, pp. 86-91."
],
"prototype": "",
"organism": "Frog virus 3",
"methylation_site": "?(5)"
},
"uba1287i": {
"target_site": "CTGCAG",
"name": "Uba1287I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1287",
"methylation_site": ""
},
"bspxii": {
"target_site": "T^GATCA",
"name": "BspXII",
"suppliers": [],
"source": "D. Dupret",
"references": [
"Zieger, M., Patillon, M., Roizes, G., Lerouge, T., Dupret, D., Jeltsch, J.M., (1987) Nucleic Acids Res., vol. 15, pp. 3919."
],
"prototype": "BclI",
"organism": "Bacillus sphaericus X",
"methylation_site": ""
},
"sdai": {
"target_site": "CCTGCA^GG",
"name": "SdaI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Sapranauskas, R., Vitkute, J., Jakubauskas, A., Unpublished observations.",
"Vitkute, J., Markauskiene, J., Maneliene, Z., Trinkunaite, L., Kiuduliene, L., Butkus, V., Janulaitis, V., Unpublished observations."
],
"prototype": "Sse8387I",
"organism": "Streptomyces diastaticus Ng7-324",
"methylation_site": ""
},
"uba1419i": {
"target_site": "AGGCCT",
"name": "Uba1419I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1419",
"methylation_site": ""
},
"mlti": {
"target_site": "AG^CT",
"name": "MltI",
"suppliers": [],
"source": "ATCC 400",
"references": [
"Christ, C., Wickberg, L., Unpublished observations.",
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "AluI",
"organism": "Micrococcus luteus",
"methylation_site": ""
},
"uba1149i": {
"target_site": "CTGCAG",
"name": "Uba1149I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1149",
"methylation_site": ""
},
"bco63i": {
"target_site": "GATNNNNATC",
"name": "Bco63I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D., Tereschenko, T., Chizikov, V.E., Andreeva, I., (1995) Gene, vol. 157, pp. 321-322.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BsaBI",
"organism": "Bacillus coagulans 63",
"methylation_site": ""
},
"bce1229i": {
"target_site": "?",
"name": "Bce1229I",
"suppliers": [],
"source": "IAM 1229",
"references": [
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "",
"organism": "Bacillus cereus",
"methylation_site": ""
},
"hpyf73i": {
"target_site": "GGNNCC",
"name": "HpyF73I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Helicobacter pylori RFL73",
"methylation_site": ""
},
"bsp74i": {
"target_site": "GATC",
"name": "Bsp74I",
"suppliers": [],
"source": "S.Y. Ye",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus 74",
"methylation_site": ""
},
"uba1228i": {
"target_site": "GGCC",
"name": "Uba1228I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1228",
"methylation_site": ""
},
"bsp29i": {
"target_site": "GGNNCC",
"name": "Bsp29I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Bacillus species RFL29",
"methylation_site": ""
},
"hpyf10iii": {
"target_site": "CCNNGG",
"name": "HpyF10III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "SecI",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"m.psadnmt1": {
"target_site": "?",
"name": "M.PsaDnmt1",
"suppliers": [],
"source": "R.L.P. Adams",
"references": [
"Jiang, C.-Z., Heard, J.E., Ratcliffe, O., Crellman, R.A., Riechmann, J.L., Haake, V., US Patent Office, 2007.",
"Pradhan, S., Cummings, M., Roberts, R.J., Adams, R.L.P., (1998) Nucleic Acids Res., vol. 26, pp. 1214-1222."
],
"prototype": "",
"organism": "Pisum sativum",
"methylation_site": "?(5)"
},
"snii": {
"target_site": "CC^WGG",
"name": "SniI",
"suppliers": [],
"source": "ATCC 19793",
"references": [
"Arif, A., Naeem, S., Sohail, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Streptomyces niveus",
"methylation_site": ""
},
"hgijii": {
"target_site": "GRGCY^C",
"name": "HgiJII",
"suppliers": [],
"source": "H. Foster",
"references": [
"Whitehead, P.R., Jacobs, D., Brown, N.L., (1986) Nucleic Acids Res., vol. 14, pp. 7031-7045."
],
"prototype": "",
"organism": "Herpetosiphon giganteus HFS101",
"methylation_site": ""
},
"uba66i": {
"target_site": "CCGCGG",
"name": "Uba66I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL66",
"methylation_site": ""
},
"tsp281i": {
"target_site": "GGCC",
"name": "Tsp281I",
"suppliers": [],
"source": "ITI 281",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Thermus species",
"methylation_site": ""
},
"uba1204i": {
"target_site": "GATC",
"name": "Uba1204I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1204",
"methylation_site": ""
},
"tsei": {
"target_site": "G^CWGC",
"name": "TseI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "D. Clark",
"references": [
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Tassoni, L., Clark, D.R., Unpublished observations.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "",
"organism": "Thermus species 93170",
"methylation_site": "?(5)"
},
"esp22i": {
"target_site": "GGYRCC",
"name": "Esp22I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL22",
"methylation_site": ""
},
"hpyf46ii": {
"target_site": "GWGCWC",
"name": "HpyF46II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Helicobacter pylori RFL46",
"methylation_site": ""
},
"uba9i": {
"target_site": "GGCC",
"name": "Uba9I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL9",
"methylation_site": ""
},
"ecorii": {
"target_site": "^CCWGG",
"name": "EcoRII",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"Toyobo Biochemicals (9/09)",
"Sigma Chemical Corporation (1/11)"
],
"source": "R.N. Yoshimori",
"references": [
"Bigger, C.H., Murray, K., Murray, N.E., (1973) Nature New Biol., vol. 244, pp. 7-10.",
"Boyer, H.W., Chow, L.T., Dugaiczyk, A., Hedgpeth, J., Goodman, H.M., (1973) Nature New Biol., vol. 244, pp. 40-43.",
"Buryanov, Y.I., Bogdarina, I.G., Bayev, A.A., (1978) FEBS Lett., vol. 88, pp. 251-254.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Reuter, M., Schneider-Mergener, J., Kupper, D., Meisel, A., Mackeldanz, P., Kruger, D.H., Schroeder, C., (1999) J. Biol. Chem., vol. 274, pp. 5213-5221.",
"Som, S., Bhagwat, A.S., Friedman, S., (1987) Nucleic Acids Res., vol. 15, pp. 313-332.",
"Som, S., Friedman, S., (1994) J. Biol. Chem., vol. 269, pp. 25986-25991.",
"Yoshimori, R.N., (1971) Ph.D. Thesis, University of California, San Francisco, USA, pp. 1-73."
],
"prototype": "",
"organism": "Escherichia coli R245",
"methylation_site": "2(5)"
},
"asp6hi": {
"target_site": "RGATCY",
"name": "Asp6HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Acidiphilium species 6H",
"methylation_site": ""
},
"sth455i": {
"target_site": "CCWGG",
"name": "Sth455I",
"suppliers": [],
"source": "G. Linden",
"references": [
"Guimont, C., Henry, P., Linden, G., (1993) Appl. Microbiol. Biotechnol., vol. 39, pp. 216-220."
],
"prototype": "EcoRII",
"organism": "Streptococcus thermophilus CNRZ 455",
"methylation_site": ""
},
"sgr1839i": {
"target_site": "TTCGAA",
"name": "Sgr1839I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Streptomyces griseoflavus 1839",
"methylation_site": ""
},
"uba71i": {
"target_site": "CTGCAG",
"name": "Uba71I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL71",
"methylation_site": ""
},
"uba86i": {
"target_site": "GGTACC",
"name": "Uba86I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Unidentified bacterium RFL86",
"methylation_site": ""
},
"aspi": {
"target_site": "GACN^NNGTC",
"name": "AspI",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "U. Mayr",
"references": [
"Bolton, B.J., Schmitz, G.G., Jarsch, M., Kessler, C., (1990) Nucleic Acids Res., vol. 18, pp. 3422."
],
"prototype": "Tth111I",
"organism": "Achromobacter species 699",
"methylation_site": ""
},
"bsezi": {
"target_site": "CTCTTC(1/4)",
"name": "BseZI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "Ksp632I",
"organism": "Bacillus species Z",
"methylation_site": ""
},
"m.zmav": {
"target_site": "?",
"name": "M.ZmaV",
"suppliers": [],
"source": "R.L. Phillips",
"references": [
"Chandler, V.L., Cone, K.C., Kaeppler, H.F., Kaeppler, S.M., Unpublished observations.",
"Kaeppler, S.M., Springer, N.M., Muszynski, M.G., International Patent Office, 2000."
],
"prototype": "",
"organism": "Zea mays",
"methylation_site": "?(5)"
},
"eagmi": {
"target_site": "G^GWCC",
"name": "EagMI",
"suppliers": [],
"source": "CAMB 2541",
"references": [
"Sohail, A., Unpublished observations.",
"Sohail, A., Mushtaq, R., Khan, E., Maqbool, T., Riazuddin, S., (1987) Pak. J. Zool., vol. 19, pp. 371-391."
],
"prototype": "AvaII",
"organism": "Enterobacter agglomerans M3",
"methylation_site": ""
},
"vpak3bi": {
"target_site": "CACGTG",
"name": "VpaK3BI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "PmaCI",
"organism": "Vibrio parahaemolyticus 4750",
"methylation_site": ""
},
"tsp273ii": {
"target_site": "GGCC",
"name": "Tsp273II",
"suppliers": [],
"source": "ITI 273",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Thermus species",
"methylation_site": ""
},
"uba1183i": {
"target_site": "GATC",
"name": "Uba1183I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1183",
"methylation_site": ""
},
"ccop84i": {
"target_site": "GATC",
"name": "CcoP84I",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vitor, J.M.B., Polisson, C., Cabrita, J., Silva, R.V.C., (1993) Acta Gastroenterol. Belg., vol. 56, pp. 41."
],
"prototype": "MboI",
"organism": "Campylobacter coli P84",
"methylation_site": ""
},
"pladi": {
"target_site": "CATCAG(21/19)",
"name": "PlaDI",
"suppliers": [],
"source": "DSM 13023",
"references": [
"Copeland, A. et al., Unpublished observations.",
"Dwinnel, E., Morgan, R.D., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Parvibaculum lavamentivorans DS-1",
"methylation_site": "?(6)"
},
"bthcani": {
"target_site": "GATC",
"name": "BthCanI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. canadensis",
"methylation_site": ""
},
"pae42i": {
"target_site": "CCGCGG",
"name": "Pae42I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Pseudomonas aeruginosa RFL42",
"methylation_site": ""
},
"hpyf5i": {
"target_site": "CTNAG",
"name": "HpyF5I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL5",
"methylation_site": ""
},
"psp56i": {
"target_site": "GGATCC",
"name": "Psp56I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Jagelavicius, M., Bitinaite, J., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Pseudomonas species RFL56",
"methylation_site": ""
},
"mlu23i": {
"target_site": "G^GATCC",
"name": "Mlu23I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Micrococcus luteus 23",
"methylation_site": ""
},
"cfr39i": {
"target_site": "YGGCCR",
"name": "Cfr39I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Citrobacter freundii RFL39",
"methylation_site": ""
},
"uba1321i": {
"target_site": "CGCG",
"name": "Uba1321I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Unidentified bacterium RFL1321",
"methylation_site": ""
},
"tbr51i": {
"target_site": "TCGA",
"name": "Tbr51I",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Al-Awadhi, S., Williams, R.A.D., (1998) Microbiology, vol. 144, pp. 167-175."
],
"prototype": "TaqI",
"organism": "Thermus brockianus JK51",
"methylation_site": ""
},
"uba1259i": {
"target_site": "GATC",
"name": "Uba1259I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1259",
"methylation_site": ""
},
"ecoo128i": {
"target_site": "G^GTNACC",
"name": "EcoO128I",
"suppliers": [],
"source": "H. Matsumoto",
"references": [
"Mise, K., Unpublished observations.",
"Miyahara, M., Mise, K., Kimizuka, F., Matsumoto, H., Terawaki, Y., (1992) Gene, vol. 113, pp. 135-136."
],
"prototype": "BstEII",
"organism": "Escherichia coli O128Ly3",
"methylation_site": ""
},
"bsari": {
"target_site": "GGCC",
"name": "BsaRI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus stearothermophilus CPW8",
"methylation_site": ""
},
"accb2i": {
"target_site": "RGCGC^Y",
"name": "AccB2I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Zhilkina, O.A., Dedkov, V.S., Shevchenko, A.V., Kileva, E.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Acinetobacter calcoaceticus B2",
"methylation_site": ""
},
"uban6i": {
"target_site": "GATNNNNATC",
"name": "UbaN6I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "BsaBI",
"organism": "Unidentified bacterium CN12-05, Nicaragua",
"methylation_site": ""
},
"bthp35i": {
"target_site": "C^TRYAG",
"name": "BthP35I",
"suppliers": [],
"source": "NEB 1563",
"references": [
"Pan, X.S., Morgan, R., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Bacillus thermoglucosidasius",
"methylation_site": ""
},
"psp29i": {
"target_site": "GGCC",
"name": "Psp29I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Pseudomonas species RFL29",
"methylation_site": ""
},
"hpyf41ii": {
"target_site": "CTNAG",
"name": "HpyF41II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL41",
"methylation_site": ""
},
"mspai": {
"target_site": "GGWCC",
"name": "MspAI",
"suppliers": [],
"source": "J.N. Reeve",
"references": [
"Morgan, R., Reeve, J., Krzycki, J., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Methanobacterium species BR10",
"methylation_site": ""
},
"hpyf7iii": {
"target_site": "GTNNAC",
"name": "HpyF7III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MjaIV",
"organism": "Helicobacter pylori RFL7",
"methylation_site": ""
},
"blidii": {
"target_site": "?",
"name": "BliDII",
"suppliers": [],
"source": "DSM 13",
"references": [
"Waschkau, B., Waldeck, J., Wieland, S., Eichstadt, R., Meinhardt, F., (2008) Appl. Microbiol. Biotechnol., vol. 78, pp. 181-188."
],
"prototype": "",
"organism": "Bacillus licheniformis DSM 13",
"methylation_site": "?(6)"
},
"ecot88i": {
"target_site": "GRGCYC",
"name": "EcoT88I",
"suppliers": [],
"source": "N. Terakado",
"references": [
"Mise, K., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli TH88",
"methylation_site": ""
},
"uba1291i": {
"target_site": "GGTNACC",
"name": "Uba1291I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BstEII",
"organism": "Unidentified bacterium RFL1291",
"methylation_site": ""
},
"llafi": {
"target_site": "?",
"name": "LlaFI",
"suppliers": [],
"source": "P. Su",
"references": [
"Su, P., Im, H., Hsieh, H., Kanga, S., Dunn, N.W., (1999) Appl. Environ. Microbiol., vol. 65, pp. 686-693."
],
"prototype": "",
"organism": "Lactococcus lactis LL42-1",
"methylation_site": "?(6)"
},
"uba1226i": {
"target_site": "GCATGC",
"name": "Uba1226I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SphI",
"organism": "Unidentified bacterium RFL1226",
"methylation_site": ""
},
"nbri": {
"target_site": "GCCGGC",
"name": "NbrI",
"suppliers": [],
"source": "ATCC 27936",
"references": [
"Qiang, B.-Q., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Nocardia brasiliensis",
"methylation_site": ""
},
"apu16i": {
"target_site": "ATCGAT",
"name": "Apu16I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Repin, V.E., Prichodko, E.A., Degtyarev, S.K., (1988) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 14, pp. 110-113."
],
"prototype": "ClaI",
"organism": "Aeromonas punctata 16",
"methylation_site": ""
},
"slu1777i": {
"target_site": "GCC^GGC",
"name": "Slu1777I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Streptomyces lusitanus 1777",
"methylation_site": ""
},
"bbiiii": {
"target_site": "CTCGAG",
"name": "BbiIII",
"suppliers": [],
"source": "T. Khosaka",
"references": [
"Khosaka, T., Kiwaki, M., (1984) FEBS Lett., vol. 177, pp. 57-60."
],
"prototype": "XhoI",
"organism": "Bifidobacterium bifidum YIT4007",
"methylation_site": ""
},
"cvini": {
"target_site": "RGCY",
"name": "CviNI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Agarwal, K., Unpublished observations."
],
"prototype": "CviJI",
"organism": "Chlorella strain NC64A (IL-2B)",
"methylation_site": ""
},
"sgai": {
"target_site": "CTCGAG",
"name": "SgaI",
"suppliers": [],
"source": "KCC S0759",
"references": [
"Shimotsu, H., Takahashi, H., Saito, H., (1980) Agric. Biol. Chem., vol. 44, pp. 1665-1666."
],
"prototype": "XhoI",
"organism": "Streptomyces ganmycicus",
"methylation_site": ""
},
"bscqii": {
"target_site": "GTCTC",
"name": "BscQII",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "BsmAI",
"organism": "Bacillus species 4304",
"methylation_site": ""
},
"ccoi": {
"target_site": "GCC^GGC",
"name": "CcoI",
"suppliers": [],
"source": "T. Kanematsu",
"references": [
"Kanematsu, T., Ozawa, O., Japanese Patent Office, 1991."
],
"prototype": "NaeI",
"organism": "Clostridium coccoides B-2",
"methylation_site": ""
},
"hpyf64iii": {
"target_site": "TCNGA",
"name": "HpyF64III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL64",
"methylation_site": ""
},
"acc113i": {
"target_site": "AGT^ACT",
"name": "Acc113I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Shevchenko, A.V., Kileva, E.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Acinetobacter calcoaceticus 113",
"methylation_site": ""
},
"bsp881i": {
"target_site": "GGCC",
"name": "Bsp881I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus IAB 881",
"methylation_site": ""
},
"bstenii": {
"target_site": "^GATC",
"name": "BstENII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus EN",
"methylation_site": ""
},
"pfl21i": {
"target_site": "CTGCA^G",
"name": "Pfl21I",
"suppliers": [],
"source": "A.V. Pertsev",
"references": [
"Pertsev, A.V., Denmukhametov, M.M., Anoshkin, A.G., Ariskina, E.V., Berezin, I.A., Solonin, A.S., Kuzmin, N.P., (2000) Prikl. Biokhim. Mikrobiol., vol. 36, pp. 13-16."
],
"prototype": "PstI",
"organism": "Pseudomonas species 21",
"methylation_site": ""
},
"accii": {
"target_site": "CG^CG",
"name": "AccII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "ATCC 49823",
"references": [
"Kita, K., Hiraoka, N., Kimizuka, F., Obayashi, A., (1984) Agric. Biol. Chem., vol. 48, pp. 531-532.",
"Zabeau, M., Roberts, R.J., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Acinetobacter calcoaceticus",
"methylation_site": ""
},
"bsp548i": {
"target_site": "CCNGG",
"name": "Bsp548I",
"suppliers": [],
"source": "ITI 439",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Bacillus species",
"methylation_site": ""
},
"ahaii": {
"target_site": "GR^CGYC",
"name": "AhaII",
"suppliers": [],
"source": "ATCC 29534",
"references": [
"Whitehead, P.R., Brown, N.L., (1985) Arch. Microbiol., vol. 141, pp. 70-74."
],
"prototype": "AcyI",
"organism": "Aphanothece halophytica",
"methylation_site": ""
},
"alfi": {
"target_site": "(10/12)GCANNNNNNTGC(12/10)",
"name": "AlfI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Zakareviciene, L., Unpublished observations.",
"Vitkute, J., Giedriene, J., Timinskas, A., Capskaja, L., Kiuduliene, L., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Acinetobacter lwoffi BH 32.",
"methylation_site": ""
},
"atu1i": {
"target_site": "CCWGG",
"name": "Atu1I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Agrobacterium tumefaciens RFL1",
"methylation_site": ""
},
"bsp153ai": {
"target_site": "CAG^CTG",
"name": "Bsp153AI",
"suppliers": [],
"source": "V. Bouriotis",
"references": [
"Kalugin, A.A., Rina, M., Eldarov, M.A., Markaki, M., Korolev, S.V., Samko, O.T., Khoroshutina, E.B., Sikolov, N.N., Bouriotis, V., (1996) Bioorg. Khim., vol. 22, pp. 108-110."
],
"prototype": "PvuII",
"organism": "Bacillus species 153A",
"methylation_site": ""
},
"cfr5i": {
"target_site": "CCWGG",
"name": "Cfr5I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL5",
"methylation_site": ""
},
"bstts5i": {
"target_site": "GAAGAC(2/6)",
"name": "BstTS5I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kovalevskaya, N.P., Zelinskaya, N.V., Zheleznaya, L.A., Matvienko, N.I., (1993) Bioorg. Khim., vol. 19, pp. 1073-1076."
],
"prototype": "BbvII",
"organism": "Bacillus stearothermophilus TS5",
"methylation_site": ""
},
"bst31ni": {
"target_site": "CCGCTC(-3/-3)",
"name": "Bst31NI",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "BsrBI",
"organism": "Bacillus stearothermophilus 31N",
"methylation_site": ""
},
"cfr31i": {
"target_site": "CCWGG",
"name": "Cfr31I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL31",
"methylation_site": ""
},
"bcivi": {
"target_site": "GTATCC(6/5)",
"name": "BciVI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1000",
"references": [
"Le, T.K.T., Vu, T.K.L., Vu, H.N., Polisson, C., Morgan, R., Unpublished observations.",
"Wei, H., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus circulans",
"methylation_site": ""
},
"bsp241i": {
"target_site": "TTCGAA",
"name": "Bsp241I",
"suppliers": [],
"source": "ITI 241",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"psc193i": {
"target_site": "?",
"name": "Psc193I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species 193",
"methylation_site": ""
},
"bssgi": {
"target_site": "CCANNNNNNTGG",
"name": "BssGI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "BstXI",
"organism": "Bacillus stearothermophilus G6",
"methylation_site": ""
},
"secii": {
"target_site": "CCGG",
"name": "SecII",
"suppliers": [],
"source": "ATCC 27170",
"references": [
"Calleja, F., Tandeau de Marsac, N., Coursin, T., van Ormondt, H., de Waard, A., (1985) Nucleic Acids Res., vol. 13, pp. 6745-6751."
],
"prototype": "HpaII",
"organism": "Synechocystis species 6701",
"methylation_site": ""
},
"van91ii": {
"target_site": "GAATTC",
"name": "Van91II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Ge, M., Gumport, R.I., Unpublished observations.",
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Vibrio anguillarum RFL91",
"methylation_site": ""
},
"bsplaiii": {
"target_site": "A^AGCTT",
"name": "BspLAIII",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Shiryaev, S.A., Zheleznaya, L.A., Matvienko, N.I., (1997) Biokhimiia, vol. 62, pp. 280-290."
],
"prototype": "HindIII",
"organism": "Bacillus species LA",
"methylation_site": ""
},
"nspji": {
"target_site": "TTCGAA",
"name": "NspJI",
"suppliers": [],
"source": "C.P. Wolk",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Nostoc species 78-12B",
"methylation_site": ""
},
"asp15i": {
"target_site": "CTCGAG",
"name": "Asp15I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Acidiphilium species 15",
"methylation_site": ""
},
"otuni": {
"target_site": "AGCT",
"name": "OtuNI",
"suppliers": [],
"source": "ATCC 25835",
"references": [
"Hall, D., Unpublished observations."
],
"prototype": "AluI",
"organism": "Oerskovia turbata",
"methylation_site": ""
},
"nli3877i": {
"target_site": "CYCGR^G",
"name": "Nli3877I",
"suppliers": [],
"source": "A.I. Melnik",
"references": [
"Melnik, A.I., Rebentish, B.A., Bolotin, A.V., Mendzhul, M.I., (1991) Mikrobiol. Zh., vol. 53, pp. 24-28."
],
"prototype": "AvaI",
"organism": "Nostoc linckia",
"methylation_site": ""
},
"van91iv": {
"target_site": "?",
"name": "Van91IV",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Vibrio anguillarum RFL91",
"methylation_site": ""
},
"m.cvipi": {
"target_site": "GC",
"name": "M.CviPI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Kladde, M.P., Simpson, R.T., Xu, M., US Patent Office, 2006.",
"Xu, M., Kladde, M.P., Van Etten, J.L., Simpson, R.T., (1998) Nucleic Acids Res., vol. 26, pp. 3961-3966."
],
"prototype": "",
"organism": "Chlorella strain NC64A (NYs-1)",
"methylation_site": "2(5)"
},
"nsui": {
"target_site": "GATC",
"name": "NsuI",
"suppliers": [],
"source": "ATCC 14221",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations."
],
"prototype": "MboI",
"organism": "Neisseria subflava",
"methylation_site": ""
},
"btu33i": {
"target_site": "GATC",
"name": "Btu33I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Burtseva, L.I., Burlak, V.A., Trusova, S.I., (1991) Mol. Gen. Mikrobiol. Virusol., vol. 11, pp. 20-22."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis 33",
"methylation_site": ""
},
"ksp22i": {
"target_site": "T^GATCA",
"name": "Ksp22I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "BclI",
"organism": "Kurthia species 22",
"methylation_site": ""
},
"bsipi": {
"target_site": "?",
"name": "BsiPI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"kpn49ki": {
"target_site": "G^AATTC",
"name": "Kpn49kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravetz, A.N., Zakharova, M.V., Beljetzkaja, I.V., Pertzev, A.V., Spivak, O.I., Solonin, A.S., (1993) Nucleic Acids Res., vol. 21, pp. 1501."
],
"prototype": "EcoRI",
"organism": "Klebsiella pneumoniae 49k",
"methylation_site": ""
},
"acpi": {
"target_site": "TT^CGAA",
"name": "AcpI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Ito, T., Sagawa, H., Kotani, H., Kishimoto, N., Sugio, T., Tano, T., Tanaka, H., (1991) Nucleic Acids Res., vol. 19, pp. 6335."
],
"prototype": "AsuII",
"organism": "Acidiphilium cryptum 25H",
"methylation_site": ""
},
"svii": {
"target_site": "TT^CGAA",
"name": "SviI",
"suppliers": [],
"source": "M. Bae",
"references": [
"Bae, M., Yun, M.-S., Kim, H.-T., Lee, K.-J., (1993) Korean J. Appl. Microbiol. Biotechnol., vol. 21, pp. 299-305.",
"Yun, M.-S., Bae, M., (1993) Proc. Mol. Biol. and Genet., vol. 8, pp. 73-74."
],
"prototype": "AsuII",
"organism": "Streptomyces violochromogenes",
"methylation_site": ""
},
"esp13i": {
"target_site": "GGYRCC",
"name": "Esp13I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL13",
"methylation_site": ""
},
"uba1295i": {
"target_site": "ATCGAT",
"name": "Uba1295I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1295",
"methylation_site": ""
},
"sgrai": {
"target_site": "CR^CCGGYG",
"name": "SgrAI",
"suppliers": [
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)"
],
"source": "U. Mayr",
"references": [
"Kong, H., Higgins, L.S., Dalton, M.A., US Patent Office, 2000.",
"Tautz, N., Kaluza, K., Frey, B., Jarsch, M., Schmitz, G.G., Kessler, C., (1990) Nucleic Acids Res., vol. 18, pp. 3087."
],
"prototype": "",
"organism": "Streptomyces griseus",
"methylation_site": "?(5)"
},
"uba1213i": {
"target_site": "CTGCAG",
"name": "Uba1213I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1213",
"methylation_site": ""
},
"bsp60i": {
"target_site": "GATC",
"name": "Bsp60I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL60",
"methylation_site": ""
},
"vnii": {
"target_site": "GGCC",
"name": "VniI",
"suppliers": [],
"source": "P. Wood",
"references": [
"Wood, P., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Vibrio nigripulchritudo",
"methylation_site": ""
},
"mali": {
"target_site": "GA^TC",
"name": "MalI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernuhin, V.A., Nayakshina, T.N., Tomilova, J.E., Degtyarev, S.K., Unpublished observations."
],
"prototype": "DpnI",
"organism": "Marinococus albus I",
"methylation_site": ""
},
"bscti": {
"target_site": "CCGCGG",
"name": "BscTI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "SacII",
"organism": "Bacillus species 106",
"methylation_site": ""
},
"eco1394i": {
"target_site": "AACNNNNNNGTGC",
"name": "Eco1394I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoKI",
"organism": "Escherichia coli EC1394",
"methylation_site": ""
},
"vpao5i": {
"target_site": "CTCTTC",
"name": "VpaO5I",
"suppliers": [],
"source": "S. Matsushita",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "Ksp632I",
"organism": "Vibrio parahaemolyticus O5",
"methylation_site": ""
},
"asp748i": {
"target_site": "CCGG",
"name": "Asp748I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Achromobacter species 748",
"methylation_site": ""
},
"cthi": {
"target_site": "TGATCA",
"name": "CthI",
"suppliers": [],
"source": "ATCC 27405",
"references": [
"Choi, K.D., Kim, K., Yoo, O.J., (1987) Sanop Misaengmul Hakhoe Chi, vol. 15, pp. 352-355."
],
"prototype": "BclI",
"organism": "Clostridium thermocellum",
"methylation_site": ""
},
"bssnai": {
"target_site": "GTA^TAC",
"name": "BssNAI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SnaI",
"organism": "Bacillus stearothermophilus NA",
"methylation_site": ""
},
"stmi": {
"target_site": "?",
"name": "StmI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Matsui, M., Mise, K., Yoshida, Y., Ishidate, M., (1986) Eisei Shikenjo Hokoku, vol. 104, pp. 92-96."
],
"prototype": "",
"organism": "Salmonella typhimurium",
"methylation_site": ""
},
"m.hinddam": {
"target_site": "GATC",
"name": "M.HindDam",
"suppliers": [],
"source": "ATCC 51907",
"references": [
"Fleischmann, R.D. et al., (1995) Science, vol. 269, pp. 496-512.",
"Fleischmann, R.D., Adams, M.D., White, O., Smith, H.O., Venter, J.C., Japanese Patent Office, 2004.",
"Roy, P.H., Smith, H.O., (1973) J. Mol. Biol., vol. 81, pp. 445-459.",
"Roy, P.H., Smith, H.O., (1973) J. Mol. Biol., vol. 81, pp. 427-444."
],
"prototype": "MboI",
"organism": "Haemophilus influenzae Rd",
"methylation_site": "2(6)"
},
"kpni": {
"target_site": "GGTAC^C",
"name": "KpnI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 49790",
"references": [
"Chatterjee, D., Longo, M., Flynn, E., Oberfelder, R., US Patent Office, 2004.",
"Chatterjee, D., Longo, M., Flynn, E., Oberfelder, R., Japanese Patent Office, 2001.",
"Chatterjee, D.K., Longo, M., Flynn, E., Oberfelder, R., US Patent Office, 2007.",
"Kiss, A., Finta, C., Venetianer, P., (1991) Nucleic Acids Res., vol. 19, pp. 3460.",
"Oberfelder, R., Longo, M., Flynn, E., Chatterjee, D., European Patent Office, 2008.",
"Smith, D.I., Blattner, F.R., Davies, J., (1976) Nucleic Acids Res., vol. 3, pp. 343-353.",
"Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R.J., (1978) Nucleic Acids Res., vol. 5, pp. 4055-4064.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "",
"organism": "Klebsiella pneumoniae OK8",
"methylation_site": "4(6)"
},
"bsp12i": {
"target_site": "CCGCGG",
"name": "Bsp12I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "SacII",
"organism": "Bacillus species RFL12",
"methylation_site": ""
},
"bsehi": {
"target_site": "AAGCTT",
"name": "BseHI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Bacillus stearothermophilus CPW5",
"methylation_site": ""
},
"cte1180i": {
"target_site": "GATC",
"name": "Cte1180I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Clostridium tetanomorphum 1180",
"methylation_site": ""
},
"cspbi": {
"target_site": "GC^GGCCGC",
"name": "CspBI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Eldarov, M.A., Rina, M., Korolev, S.V., Markaki, M., Kalugin, A.A., Omelyanuk, N.M., Skryabin, K.G., Bouriotis, V., (1998) Biochem. Mol. Biol. Int., vol. 44, pp. 433-441."
],
"prototype": "NotI",
"organism": "Corynebacterium species B",
"methylation_site": ""
},
"bsp143ii": {
"target_site": "RGCGC^Y",
"name": "Bsp143II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations.",
"Lubiene, J., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus species RFL143",
"methylation_site": ""
},
"hhgi": {
"target_site": "GGCC",
"name": "HhgI",
"suppliers": [],
"source": "ATCC 19416",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Haemophilus haemoglobinophilus",
"methylation_site": ""
},
"sseai": {
"target_site": "GG^CGCC",
"name": "SseAI",
"suppliers": [],
"source": "V. Bouriotis",
"references": [
"Rina, M., Karagouni, A., Pagomenou, M., Tsigos, I., Bouriotis, V., (1991) Nucleic Acids Res., vol. 19, pp. 6341."
],
"prototype": "NarI",
"organism": "Streptomyces species",
"methylation_site": ""
},
"npehemi": {
"target_site": "?",
"name": "NpeHEMI",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Nodularia species HEM",
"methylation_site": ""
},
"haeiii": {
"target_site": "GG^CC",
"name": "HaeIII",
"suppliers": [
"Invitrogen Corporation (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 11116",
"references": [
"Bron, S., Murray, K., (1975) Mol. Gen. Genet., vol. 143, pp. 25-33.",
"Mann, M.B., Smith, H.O., (1977) Nucleic Acids Res., vol. 4, pp. 4211-4221.",
"Middleton, J.H., Edgell, M.H., Hutchison, C.A. III, (1972) J. Virol., vol. 10, pp. 42-50.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009."
],
"prototype": "",
"organism": "Haemophilus aegyptius",
"methylation_site": "3(5)"
},
"splii": {
"target_site": "GACNNNGTC",
"name": "SplII",
"suppliers": [],
"source": "M. Kawamura",
"references": [
"Kawamura, M., Sakakibara, M., Watanabe, T., Kita, K., Hiraoka, N., Obayashi, A., Takagi, M., Yano, K., (1986) Nucleic Acids Res., vol. 14, pp. 1985-1989."
],
"prototype": "Tth111I",
"organism": "Spirulina platensis",
"methylation_site": ""
},
"hpyf67iii": {
"target_site": "GGATG",
"name": "HpyF67III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "FokI",
"organism": "Helicobacter pylori RFL67",
"methylation_site": ""
},
"tvei": {
"target_site": "?",
"name": "TveI",
"suppliers": [],
"source": "IFO 14567",
"references": [
"Inagaki, K., Morimoto, Y., Sugio, T., Tano, T., Unpublished observations."
],
"prototype": "",
"organism": "Thiobacillus versutus",
"methylation_site": ""
},
"bspoi": {
"target_site": "GCTAG^C",
"name": "BspOI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G337",
"references": [
"Vitkute, J., Lapcinskaja, S., Trinkunaite, L., Capskaja, L., Zakareviciene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "NheI",
"organism": "Bacillus sphaericus RFL3",
"methylation_site": ""
},
"hpyf33ii": {
"target_site": "GGCC",
"name": "HpyF33II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL33",
"methylation_site": ""
},
"aspdi": {
"target_site": "CYCGRG",
"name": "AspDI",
"suppliers": [],
"source": "ATCC 27898",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaI",
"organism": "Anabaena species",
"methylation_site": ""
},
"eco78i": {
"target_site": "GGC^GCC",
"name": "Eco78I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Butkus, V., Kazlauskiene, R., Gilvonauskaite, R., Petrusyte, M., Janulaitis, A., (1985) Bioorg. Khim., vol. 11, pp. 1572-1573."
],
"prototype": "NarI",
"organism": "Escherichia coli RFL78",
"methylation_site": ""
},
"bfuai": {
"target_site": "ACCTGC(4/8)",
"name": "BfuAI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1292",
"references": [
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nkenfou, C., Polisson, C., Unpublished observations.",
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "BspMI",
"organism": "Bacillus fusiformis 1083",
"methylation_site": "?(5)"
},
"ncui": {
"target_site": "GAAGA(8/7)",
"name": "NcuI",
"suppliers": [],
"source": "ATCC 14688",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations.",
"Furmanek, B., Gromek, K., Sektas, M., Kaczorowski, T., (2001) FEMS Microbiol. Lett., vol. 196, pp. 171-176.",
"Furmanek, B., Kaczorowski, T., Unpublished observations.",
"Furmanek, B., Sektas, M., Wons, E., Kaczorowski, T., (2007) Res. Microbiol., vol. 158, pp. 164-174.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "MboII",
"organism": "Neisseria cuniculi",
"methylation_site": "5(6)"
},
"bsp91i": {
"target_site": "GATC",
"name": "Bsp91I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL91",
"methylation_site": ""
},
"pspppi": {
"target_site": "RG^GWCCY",
"name": "PspPPI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Kileva, E.V., Abdurashitov, M.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "PpuMI",
"organism": "Pseudomonas species PP",
"methylation_site": ""
},
"sth134i": {
"target_site": "C^CGG",
"name": "Sth134I",
"suppliers": [],
"source": "D.K.Y. Solaiman",
"references": [
"Hill, C., (1993) FEMS Microbiol. Rev., vol. 12, pp. 87-108.",
"Solaiman, D.K.Y., Somkuti, G.A., (1990) FEMS Microbiol. Lett., vol. 67, pp. 261-266."
],
"prototype": "HpaII",
"organism": "Streptococcus thermophilus strain 134",
"methylation_site": ""
},
"tteai": {
"target_site": "GGCC",
"name": "TteAI",
"suppliers": [],
"source": "ATCC 27914",
"references": [
"Siegelman, B., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Tolypothrix tenuis",
"methylation_site": ""
},
"cdicd6i": {
"target_site": "GGNCC",
"name": "CdiCD6I",
"suppliers": [],
"source": "N. Minton",
"references": [
"Minton, N.P., Purdy, D.A., Elmore, M.J., O'Keeffe, K.M.T., International Patent Office, 2001.",
"Purdy, D., O'Keeffe, T.A., Elmore, M., Herbert, M., McLeod, A., Bokori-Brown, M., Ostrowski, A., Minton, N.P., (2002) Mol. Microbiol., vol. 46, pp. 439-452."
],
"prototype": "AsuI",
"organism": "Clostridium difficile CD6",
"methylation_site": "4(5)"
},
"msci": {
"target_site": "TGG^CCA",
"name": "MscI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "NEB 502",
"references": [
"Meda, M.M., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., (1989) Nucleic Acids Res., vol. 17, pp. 5858."
],
"prototype": "BalI",
"organism": "Micrococcus species",
"methylation_site": ""
},
"uba1337i": {
"target_site": "CTGCAG",
"name": "Uba1337I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1337",
"methylation_site": ""
},
"bsixi": {
"target_site": "AT^CGAT",
"name": "BsiXI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species X",
"methylation_site": ""
},
"sthdi": {
"target_site": "GGTACC",
"name": "SthDI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY197",
"methylation_site": ""
},
"gsui": {
"target_site": "CTGGAG(16/14)",
"name": "GsuI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "M.S. Loytsianskaya",
"references": [
"Janulaitis, A., Bitinaite, J., Jaskeleviciene, B., (1983) FEBS Lett., vol. 151, pp. 243-247.",
"Petrusyte, M.P., Bitinaite, J.B., Kersulyte, D.R., Menkevicius, S.J., Butkus, V.V., Janulaitis, A., (1987) Dokl. Akad. Nauk., vol. 295, pp. 1250-1253.",
"Vaisvila, R., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Gluconobacter suboxydans H-15T",
"methylation_site": ""
},
"pssii": {
"target_site": "?",
"name": "PssII",
"suppliers": [],
"source": "R.A. Makula",
"references": [
"Makula, R.A., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas species",
"methylation_site": ""
},
"hpyf53ii": {
"target_site": "GTAC",
"name": "HpyF53II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL53",
"methylation_site": ""
},
"cjep659iv": {
"target_site": "CACNNNNNNNGAA",
"name": "CjeP659IV",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vital, J., Vitor, J.M.B., Unpublished observations."
],
"prototype": "",
"organism": "Campylobacter jejuni P659",
"methylation_site": ""
},
"fsp4hi": {
"target_site": "GC^NGC",
"name": "Fsp4HI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chmuzh, E.V., Abdurashitov, M.A., Gonchar, D.A., Kashirina, J.G., Dedkov, V.S., Degtyarev, S.K., Unpublished observations.",
"Chmuzh, E.V., Kashirina, Y.G., Tomilova, Y.E., Chernukhin, V.A., Okhapkina, S.S., Gonchar, D.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., (2007) Mol. Biol. (Mosk), vol. 41, pp. 43-50.",
"Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Fnu4HI",
"organism": "Flavobacterium species 4H",
"methylation_site": "2(5)"
},
"saki": {
"target_site": "CCGCGG",
"name": "SakI",
"suppliers": [],
"source": "ATCC 13480",
"references": [
"Schneider, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces akiyoshiensis",
"methylation_site": ""
},
"bst1473ii": {
"target_site": "RGCGCY",
"name": "Bst1473II",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus stearothermophilus 1473",
"methylation_site": ""
},
"esabs9i": {
"target_site": "CGCG",
"name": "EsaBS9I",
"suppliers": [],
"source": "R.D. Morgan",
"references": [
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "FnuDII",
"organism": "Environmental sample BS #9",
"methylation_site": "?(4)"
},
"ppii": {
"target_site": "(7/12)GAACNNNNNCTC(13/8)",
"name": "PpiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Jurenaite-Urbanaviciene, S., (2008) Ph.D. Thesis, Vilnius University, pp. 1-46.",
"Jurenaite-Urbanaviciene, S., Serksnaite, J., Kriukiene, E., Giedriene, J., Venclovas, C., Lubys, A., (2007) Proc. Natl. Acad. Sci. U. S. A., vol. 104, pp. 10358-10363.",
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Kairaitiene, R., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas putida Jo 4-731",
"methylation_site": "3(6),-2(6)"
},
"ngodi": {
"target_site": "?",
"name": "NgoDI",
"suppliers": [],
"source": "J.K. Davies",
"references": [
"Duff, M.K., Davies, J.K., (1988) Gonococci and Meningococci, ed. Poolman, J.T., Zanen, H.C., Meyer, T.F., Heckels, J.E., Makela, P.R.H. (Kluwer Academic Publishers, Dordrecht, The Netherlands), pp. 251-256."
],
"prototype": "",
"organism": "Neisseria gonorrhoeae JKD211",
"methylation_site": ""
},
"fnudi": {
"target_site": "GG^CC",
"name": "FnuDI",
"suppliers": [],
"source": "M. Smith",
"references": [
"Lui, A.C.P., McBride, B.C., Vovis, G.F., Smith, M., (1979) Nucleic Acids Res., vol. 6, pp. 1-15.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Van Cott, E., Wilson, G., European Patent Office, 1987.",
"Van Cott, E.M., Wilson, G.G., US Patent Office, 1991."
],
"prototype": "HaeIII",
"organism": "Fusobacterium nucleatum D",
"methylation_site": "?(5)"
},
"bmet110i": {
"target_site": "C^YCGRG",
"name": "BmeT110I",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "Takara T110",
"references": [
"Sawaragi, H., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Bacillus megaterium T110",
"methylation_site": ""
},
"saugi": {
"target_site": "GATC",
"name": "SauGI",
"suppliers": [],
"source": "E.E. Stobberingh",
"references": [
"Stobberingh, E.E., Schiphof, R., Sussenbach, J.S., (1977) J. Bacteriol., vol. 131, pp. 645-649."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus 1030",
"methylation_site": ""
},
"baei": {
"target_site": "(10/15)ACNNNNGTAYC(12/7)",
"name": "BaeI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 659",
"references": [
"Sears, L.E., Zhou, B., Aliotta, J.M., Morgan, R.D., Kong, H., (1996) Nucleic Acids Res., vol. 24, pp. 3590-3592.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"bspf105i": {
"target_site": "CCSGG",
"name": "BspF105I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "CauII",
"organism": "Bacillus species cy105",
"methylation_site": ""
},
"bsifi": {
"target_site": "?",
"name": "BsiFI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"ndei": {
"target_site": "CA^TATG",
"name": "NdeI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "NRCC 31009",
"references": [
"Benner, J.S., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Silber, K.R., Polisson, C., Rees, P.A., Benner, J.S., (1988) Gene, vol. 74, pp. 43-44.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Watson, R.J., Schildkraut, I., Qiang, B.-Q., Martin, S.M., Visentin, L.P., (1982) FEBS Lett., vol. 150, pp. 114-116."
],
"prototype": "",
"organism": "Neisseria denitrificans",
"methylation_site": "?(6)"
},
"hin5ii": {
"target_site": "GGNCC",
"name": "Hin5II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "AsuI",
"organism": "Haemophilus influenzae RFL5",
"methylation_site": ""
},
"clui": {
"target_site": "?",
"name": "CluI",
"suppliers": [],
"source": "H. Mayer",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Caryophanon latum RII",
"methylation_site": ""
},
"vcho44i": {
"target_site": "AGGCCT",
"name": "VchO44I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "StuI",
"organism": "Vibrio cholerae 112-73",
"methylation_site": ""
},
"eco83i": {
"target_site": "CTGCAG",
"name": "Eco83I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "PstI",
"organism": "Escherichia coli RFL83",
"methylation_site": ""
},
"pgl34i": {
"target_site": "CACGTG",
"name": "Pgl34I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PmaCI",
"organism": "Pseudomonas glathei RFL34",
"methylation_site": ""
},
"rlei": {
"target_site": "?",
"name": "RleI",
"suppliers": [],
"source": "J. Beringer",
"references": [
"Winkler, K., Rosch, A., Unpublished observations."
],
"prototype": "",
"organism": "Rhizobium leguminosarum 300",
"methylation_site": ""
},
"m.tpai": {
"target_site": "GATC",
"name": "M.TpaI",
"suppliers": [],
"source": "L.V. Stamm",
"references": [
"Stamm, L.V., Greene, S.R., Barnes, N.Y., Bergen, H.L., Hardham, J.M., (1997) FEMS Microbiol. Lett., vol. 155, pp. 115-119."
],
"prototype": "MboI",
"organism": "Treponema pallidum",
"methylation_site": "2(6)"
},
"cfuii": {
"target_site": "CTGCA^G",
"name": "CfuII",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lebenka, A.Yu., Chitavichyus, D.B., (1988) Biokhimiia, vol. 53, pp. 1895-1899."
],
"prototype": "PstI",
"organism": "Caulobacter fusiformis BC-25",
"methylation_site": ""
},
"finii": {
"target_site": "CCGG",
"name": "FinII",
"suppliers": [],
"source": "NEB 382",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Flavobacterium indologenes",
"methylation_site": ""
},
"hpyv": {
"target_site": "TCGA",
"name": "HpyV",
"suppliers": [],
"source": "ATCC 49503",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori 60190",
"methylation_site": ""
},
"smimii": {
"target_site": "GATATC",
"name": "SmiMII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Najakshina, T.N., Degtyarev, S.K., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Sphingobacterium mizutae M",
"methylation_site": ""
},
"bsiti": {
"target_site": "?",
"name": "BsiTI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"rcai": {
"target_site": "T^CATGA",
"name": "RcaI",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "M.M. Wijdenbosch",
"references": [
"Wijdenbosch, M.M., Unpublished observations."
],
"prototype": "BspHI",
"organism": "Rhodococcus capsulatum",
"methylation_site": ""
},
"bstz12i": {
"target_site": "?",
"name": "BstZ12I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus 35O",
"methylation_site": ""
},
"msp20i": {
"target_site": "TGG^CCA",
"name": "Msp20I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernov, A.P., Belichenko, O.A., Rechkunova, N.I., Andreeva, I.S., Repin, V.E., Degtyarev, S.K., Russian Patent Office, 1993."
],
"prototype": "BalI",
"organism": "Micrococcus species",
"methylation_site": ""
},
"bssgii": {
"target_site": "GATC",
"name": "BssGII",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus G6",
"methylation_site": ""
},
"ngomviii": {
"target_site": "GGTGA",
"name": "NgoMVIII",
"suppliers": [],
"source": "M. So",
"references": [
"Chien, H.R., (1991) Ph.D. Thesis, University of Maryland, USA, pp. 1-126.",
"Stein, D.C., Gunn, J.S., Radlinska, M., Piekarowicz, A., (1995) Gene, vol. 157, pp. 19-22."
],
"prototype": "HphI",
"organism": "Neisseria gonorrhoeae MS11",
"methylation_site": "?(5)"
},
"uba1399i": {
"target_site": "CTGCAG",
"name": "Uba1399I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1399",
"methylation_site": ""
},
"ecaii": {
"target_site": "CCWGG",
"name": "EcaII",
"suppliers": [],
"source": "DSM 30056",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae",
"methylation_site": ""
},
"uba1371i": {
"target_site": "AGGCCT",
"name": "Uba1371I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1371",
"methylation_site": ""
},
"m.pmuadam": {
"target_site": "GATC",
"name": "M.PmuADam",
"suppliers": [],
"source": "ATCC 11039",
"references": [
"Chen, L., Paulsen, D.B., Lawrence, M.L., Unpublished observations.",
"Chen, L., Paulsen, D.B., Scruggs, D.W., Banes, M.M., Reeks, B.Y., Lawrence, M.L., (2003) Microbiology, vol. 149, pp. 2283-2290."
],
"prototype": "MboI",
"organism": "Pasteurella multocida",
"methylation_site": "?(6)"
},
"stysji": {
"target_site": "GAGNNNNNNGTRC",
"name": "StySJI",
"suppliers": [],
"source": "N.E. Murray",
"references": [
"Gann, A.A.F., Campbell, A.J.B., Collins, J.F., Coulson, A.F.W., Murray, N.E., (1987) Mol. Microbiol., vol. 1, pp. 13-22."
],
"prototype": "",
"organism": "Salmonella hybrid",
"methylation_site": "?(6)"
},
"apabi": {
"target_site": "GCANNNNN^TGC",
"name": "ApaBI",
"suppliers": [],
"source": "J. Grones",
"references": [
"Grones, J., Turna, J., (1991) Biologia (Bratisl), vol. 46, pp. 1103-1108."
],
"prototype": "",
"organism": "Acetobacter pasteurianus B",
"methylation_site": ""
},
"aseii": {
"target_site": "CC^SGG",
"name": "AseII",
"suppliers": [],
"source": "NEB 448",
"references": [
"Polisson, C., Morgan, R.D., (1988) Nucleic Acids Res., vol. 16, pp. 10365."
],
"prototype": "CauII",
"organism": "Aquaspirillum serpens",
"methylation_site": ""
},
"aini": {
"target_site": "CTGCAG",
"name": "AinI",
"suppliers": [],
"source": "CCAP 1446/1a",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Anabaena inequalis",
"methylation_site": ""
},
"aosii": {
"target_site": "GR^CGYC",
"name": "AosII",
"suppliers": [],
"source": "CCAP 1403/11",
"references": [
"de Waard, A., van Beveren, C.P., Duyvesteyn, M., van Ormondt, H., (1979) FEBS Lett., vol. 101, pp. 71-76."
],
"prototype": "AcyI",
"organism": "Anabaena oscillarioides",
"methylation_site": ""
},
"csp45i": {
"target_site": "TT^CGAA",
"name": "Csp45I",
"suppliers": [
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)"
],
"source": "T. Schoenfeld",
"references": [
"Schoenfeld, T., Mead, D.A., Fiandt, M., (1989) Nucleic Acids Res., vol. 17, pp. 4417."
],
"prototype": "AsuII",
"organism": "Clostridium sporogenes",
"methylation_site": ""
},
"lla2614i": {
"target_site": "?",
"name": "Lla2614I",
"suppliers": [],
"source": "M. Chopin",
"references": [
"Schouler, C., Clier, F., Luisa, A., Lerayer, L., Ehrlich, S.D., Chopin, M.-C., (1998) J. Bacteriol., vol. 180, pp. 407-411."
],
"prototype": "",
"organism": "Lactococcus lactis pIL2614",
"methylation_site": "?(6)"
},
"uban7i": {
"target_site": "CTCTTC",
"name": "UbaN7I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "Ksp632I",
"organism": "Unidentified bacterium TOT-01, Nicaragua",
"methylation_site": ""
},
"uba1339i": {
"target_site": "GGATCC",
"name": "Uba1339I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1339",
"methylation_site": ""
},
"bsp107i": {
"target_site": "CTGCAG",
"name": "Bsp107I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species RFL107",
"methylation_site": ""
},
"cfr56i": {
"target_site": "GGTCTC",
"name": "Cfr56I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Citrobacter freundii RFL56",
"methylation_site": ""
},
"bme2494i": {
"target_site": "GATC",
"name": "Bme2494I",
"suppliers": [],
"source": "NEB 1399",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus megaterium 2494",
"methylation_site": ""
},
"sal1974i": {
"target_site": "CTCGAG",
"name": "Sal1974I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces albus 1974",
"methylation_site": ""
},
"noci": {
"target_site": "CTGCAG",
"name": "NocI",
"suppliers": [],
"source": "ATCC 14629",
"references": [
"Comb, D.G., Schildkraut, I., Unpublished observations."
],
"prototype": "PstI",
"organism": "Nocardia otitidis-caviarum",
"methylation_site": ""
},
"bsc91i": {
"target_site": "GAAGAC(2/6)",
"name": "Bsc91I",
"suppliers": [],
"source": "T. Schoenfeld",
"references": [
"Schoenfeld, T., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus species",
"methylation_site": ""
},
"chii": {
"target_site": "?",
"name": "ChiI",
"suppliers": [],
"source": "R. Hansen",
"references": [
"Hansen, R., Unpublished observations."
],
"prototype": "",
"organism": "Clostridium histolyticum",
"methylation_site": ""
},
"bstz8i": {
"target_site": "CGATCG",
"name": "BstZ8I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Bacillus stearothermophilus 29K",
"methylation_site": ""
},
"plui": {
"target_site": "AGGCCT",
"name": "PluI",
"suppliers": [],
"source": "Viikki 152",
"references": [
"Mattila, P., Pitkanen, K., Unpublished observations."
],
"prototype": "StuI",
"organism": "Pseudomonas fluorescens",
"methylation_site": ""
},
"pph1579i": {
"target_site": "GGNCC",
"name": "Pph1579I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Photobacterium phosphoreum 1579",
"methylation_site": ""
},
"hpyf69i": {
"target_site": "ACNGT",
"name": "HpyF69I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL69",
"methylation_site": ""
},
"cte1i": {
"target_site": "CCGCGG",
"name": "Cte1I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Comamonas testosteroni RFL1",
"methylation_site": ""
},
"uba1153i": {
"target_site": "GGCC",
"name": "Uba1153I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1153",
"methylation_site": ""
},
"nmufi": {
"target_site": "GCCGGC",
"name": "NmuFI",
"suppliers": [],
"source": "ATCC 19697",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Neisseria mucosa",
"methylation_site": ""
},
"xgl3220i": {
"target_site": "CGATCG",
"name": "Xgl3220I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Xanthomonas glycinae 3220",
"methylation_site": ""
},
"pini": {
"target_site": "AGTACT",
"name": "PinI",
"suppliers": [],
"source": "ATCC 14036",
"references": [
"Klein, S., Roberts, R.J., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Pseudomonas indigofera",
"methylation_site": ""
},
"bse19i": {
"target_site": "CCATGG",
"name": "Bse19I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "NcoI",
"organism": "Bacillus species 19",
"methylation_site": ""
},
"uba1445i": {
"target_site": "GGNNCC",
"name": "Uba1445I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Unidentified bacterium RFL1445",
"methylation_site": ""
},
"yenbi": {
"target_site": "CTGCAG",
"name": "YenBI",
"suppliers": [],
"source": "T. Maruyama",
"references": [
"Miyahara, M., Maruyama, T., Wake, A., Mise, K., (1988) Appl. Environ. Microbiol., vol. 54, pp. 577-580."
],
"prototype": "PstI",
"organism": "Yersinia enterocolitica 08 Bi1212",
"methylation_site": ""
},
"uba1162i": {
"target_site": "GCATGC",
"name": "Uba1162I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SphI",
"organism": "Unidentified bacterium RFL1162",
"methylation_site": ""
},
"asi256i": {
"target_site": "G^ATC",
"name": "Asi256I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Ushakova, T.A., Puchkova, L.I., Gutorov, V.V., Totmenina, O.D., Repin, V.E., (2008) Prikl. Biokhim. Mikrobiol., vol. 44, pp. 28-31."
],
"prototype": "MboI",
"organism": "Arthrobacter species strain Ck256",
"methylation_site": ""
},
"uba1115i": {
"target_site": "CTGCAG",
"name": "Uba1115I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1115",
"methylation_site": ""
},
"hpyf58i": {
"target_site": "ACNGT",
"name": "HpyF58I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL58",
"methylation_site": ""
},
"bsp67i": {
"target_site": "^GATC",
"name": "Bsp67I",
"suppliers": [],
"source": "S.Y. Ye",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus 67",
"methylation_site": ""
},
"bmii": {
"target_site": "GGN^NCC",
"name": "BmiI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 62",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"hpyf74i": {
"target_site": "ACNGT",
"name": "HpyF74I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL74",
"methylation_site": ""
},
"mho2965i": {
"target_site": "GCGC",
"name": "Mho2965I",
"suppliers": [],
"source": "A. Duarte",
"references": [
"Paquete, C.M.V., Duarte, A., Vitor, J.M.B., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Mycoplasma hominis 2965",
"methylation_site": ""
},
"hpyf21i": {
"target_site": "CTNAG",
"name": "HpyF21I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL21",
"methylation_site": ""
},
"bstzi": {
"target_site": "C^GGCCG",
"name": "BstZI",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations.",
"King, K., Unpublished observations."
],
"prototype": "XmaIII",
"organism": "Bacillus stearothermophilus Z130",
"methylation_site": ""
},
"bpugci": {
"target_site": "GCTNAGC",
"name": "BpuGCI",
"suppliers": [],
"source": "NEB 1034",
"references": [
"Osterfield, T., Unpublished observations."
],
"prototype": "EspI",
"organism": "Bacillus pumilis GC subgroup 8",
"methylation_site": ""
},
"bspbi": {
"target_site": "CTGCA^G",
"name": "BspBI",
"suppliers": [],
"source": "R. Mullings",
"references": [
"Mullings, R., Evans, L.R., Brown, N.L., (1986) FEMS Microbiol. Lett., vol. 37, pp. 237-240."
],
"prototype": "PstI",
"organism": "Bacillus sphaericus JL14",
"methylation_site": ""
},
"mlu2300i": {
"target_site": "CCWGG",
"name": "Mlu2300I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Micrococcus luteus 2300",
"methylation_site": ""
},
"bbvci": {
"target_site": "CCTCAGC(-5/-2)",
"name": "BbvCI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1030",
"references": [
"Ge, L., Unpublished observations.",
"Heiter, D., Lunnen, K., Wilson, G.G., US Patent Office, 2006.",
"Heiter, D.F., Lunnen, K.D., Wilson, G.G., (2005) J. Mol. Biol., vol. 348, pp. 631-640.",
"Krotee, S., Ganatra, M., Grandoni, R., Unpublished observations.",
"Lunnen, K.D., Heiter, D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Bacillus brevis",
"methylation_site": "?(5)"
},
"hpyf10ii": {
"target_site": "GANTC",
"name": "HpyF10II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"bca1259i": {
"target_site": "GGATCC",
"name": "Bca1259I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Bacillus caldotenax 1259",
"methylation_site": ""
},
"uba1397i": {
"target_site": "CTCGAG",
"name": "Uba1397I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1397",
"methylation_site": ""
},
"blihki": {
"target_site": "CC^TNAGG",
"name": "BliHKI",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Wu, S.Y., Lee, K.F., Kam, K.M., Shaw, P.C., (1993) Biosci. Biotechnol. Biochem., vol. 57, pp. 1193-1194."
],
"prototype": "SauI",
"organism": "Bacillus licheniformis HK",
"methylation_site": ""
},
"cdiai": {
"target_site": "GGNCC",
"name": "CdiAI",
"suppliers": [],
"source": "ATCC 25408",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Citrobacter diversus",
"methylation_site": ""
},
"bce71i": {
"target_site": "GGCC",
"name": "Bce71I",
"suppliers": [],
"source": "S.Y. Ye",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "HaeIII",
"organism": "Bacillus cereus 71",
"methylation_site": ""
},
"uba1429i": {
"target_site": "GGCC",
"name": "Uba1429I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1429",
"methylation_site": ""
},
"bcgi": {
"target_site": "(10/12)CGANNNNNNTGC(12/10)",
"name": "BcgI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 566",
"references": [
"Kong, H., Unpublished observations.",
"Kong, H., Morgan, R.D., Maunus, R.E., Schildkraut, I., (1993) Nucleic Acids Res., vol. 21, pp. 987-991.",
"Kong, H., Roemer, S.E., Waite-Rees, P.A., Benner, J.S., Wilson, G.G., Nwankwo, D.O., (1994) J. Biol. Chem., vol. 269, pp. 683-690.",
"Kong, H., Schildkraut, I., European Patent Office, 1994."
],
"prototype": "",
"organism": "Bacillus coagulans",
"methylation_site": "3(6),-3(6)"
},
"bshli": {
"target_site": "GATATC",
"name": "BshLI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Bacillus sphaericus DC2",
"methylation_site": ""
},
"xori": {
"target_site": "CTGCAG",
"name": "XorI",
"suppliers": [],
"source": "M. Ehrlich",
"references": [
"Wang, R.Y.-H., Shedlarski, J.G., Farber, M.B., Kuebbing, D., Ehrlich, M., (1980) Biochim. Biophys. Acta, vol. 606, pp. 371-385."
],
"prototype": "PstI",
"organism": "Xanthomonas oryzae",
"methylation_site": ""
},
"slbi": {
"target_site": "GGTCTC",
"name": "SlbI",
"suppliers": [],
"source": "Viikki 329",
"references": [
"Mattila, P., Pitkanen, K., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Streptomyces albidoflavus",
"methylation_site": ""
},
"hpyf10iv": {
"target_site": "GTAC",
"name": "HpyF10IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"rmai": {
"target_site": "C^TAG",
"name": "RmaI",
"suppliers": [],
"source": "ITI 378",
"references": [
"Hjorleifsdottir, S., Pelursdottir, S., Kristjansson, J.K., Korpela, J., Torsti, A.-M., Mattila, P., (1992) Thermophiles Sci. Technol., vol. 20, pp. 17.",
"Ronka, J., Hjorleifsdottir, S., Tenkanen, T., Pitkanen, K., Mattila, P., Kristjansson, J.K., (1991) Nucleic Acids Res., vol. 19, pp. 2789."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"sfli": {
"target_site": "CTGCA^G",
"name": "SflI",
"suppliers": [],
"source": "UC 5066",
"references": [
"Jiang, B.D., Myers, P., Unpublished observations."
],
"prototype": "PstI",
"organism": "Streptoverticillium flavopersicum",
"methylation_site": ""
},
"sspm1ii": {
"target_site": "GRCGYC",
"name": "SspM1II",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "AcyI",
"organism": "Spirulina species M1",
"methylation_site": ""
},
"rma509i": {
"target_site": "CTAG",
"name": "Rma509I",
"suppliers": [],
"source": "ITI 509",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"uba1357i": {
"target_site": "GRGCYC",
"name": "Uba1357I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1357",
"methylation_site": ""
},
"bmebi": {
"target_site": "CTGCAG",
"name": "BmeBI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus megaterium B78",
"methylation_site": ""
},
"hgii": {
"target_site": "GR^CGYC",
"name": "HgiI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Wersig, U., Bolton, B., Frey, B., Jarsch, M., Kessler, C., Unpublished observations."
],
"prototype": "AcyI",
"organism": "Herpetosiphon giganteus 3303",
"methylation_site": ""
},
"clci": {
"target_site": "CTGCAG",
"name": "ClcI",
"suppliers": [],
"source": "ATCC 29412",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Cylindrospermum lichenforme",
"methylation_site": ""
},
"ahab1i": {
"target_site": "GGNCC",
"name": "AhaB1I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh., (1990) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 17-18."
],
"prototype": "AsuI",
"organism": "Alteromonas haloplanktis B1",
"methylation_site": ""
},
"nsii": {
"target_site": "ATGCA^T",
"name": "NsiI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"Nippon Gene Co., Ltd. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)"
],
"source": "ATCC 29256",
"references": [
"Longo, M.C., Smith, M.D., US Patent Office, 1995.",
"Schildkraut, I., Jones, G., Parker, P., Grandoni, R., Comb, D.G., Unpublished observations.",
"Xia, Y., Van Etten, J.L., Dobos, P., Ling, Y.Y., Krell, P.J., (1993) Virology, vol. 196, pp. 817-824.",
"Zhu, Z., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Neisseria sicca",
"methylation_site": ""
},
"hpyf10v": {
"target_site": "GGCC",
"name": "HpyF10V",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"hpyf10i": {
"target_site": "GCGC",
"name": "HpyF10I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Helicobacter pylori RFL10",
"methylation_site": ""
},
"bstti": {
"target_site": "CCANNNNNNTGG",
"name": "BstTI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "BstXI",
"organism": "Bacillus stearothermophilus T12",
"methylation_site": ""
},
"asp86i": {
"target_site": "ATCGAT",
"name": "Asp86I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "ClaI",
"organism": "Anabaena species 86",
"methylation_site": ""
},
"hpy51i": {
"target_site": "^GTSAC",
"name": "Hpy51I",
"suppliers": [],
"source": "M.J. Cho",
"references": [
"Baik, S.C., Lee, W.K., Song, J.Y., Choi, Y.J., Rye, B.D., Choi, S.H., Cho, M.J., Rhee, K.H., (1998) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 0, pp. 120.",
"Kim, S. et al., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori 51",
"methylation_site": ""
},
"bsisi": {
"target_site": "C^CGG",
"name": "BsiSI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "D. Clark",
"references": [
"Rina, M., Bouriotis, V., (1990) Nucleic Acids Res., vol. 18, pp. 1654."
],
"prototype": "HpaII",
"organism": "Bacillus species",
"methylation_site": ""
},
"eco65i": {
"target_site": "AAGCTT",
"name": "Eco65I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Escherichia coli RFL65",
"methylation_site": ""
},
"uba1111i": {
"target_site": "CCGCGG",
"name": "Uba1111I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1111",
"methylation_site": ""
},
"cpapi": {
"target_site": "GATC",
"name": "CpaPI",
"suppliers": [],
"source": "NRCC 33011",
"references": [
"Venetianer, P., Unpublished observations."
],
"prototype": "MboI",
"organism": "Clostridium pasteurianum",
"methylation_site": ""
},
"kteai": {
"target_site": "CCCGGG",
"name": "KteAI",
"suppliers": [],
"source": "ATCC 33631",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "SmaI",
"organism": "Klebsiella terrigena",
"methylation_site": ""
},
"bslfi": {
"target_site": "GGGAC(10/14)",
"name": "BslFI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Nadeev, A.N., Kashirina, J.G., Nayakshina, T.N., Dedkov, V.S., Mezentseva, N.V., Tomilova, J.E., Degtyarev, S.K., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus stearothermophilus FI",
"methylation_site": ""
},
"eco240i": {
"target_site": "GGTCTC",
"name": "Eco240I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL240",
"methylation_site": ""
},
"esawc1i": {
"target_site": "GGCC",
"name": "EsaWC1I",
"suppliers": [],
"source": "R.D. Morgan",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Environmental sample WC1",
"methylation_site": ""
},
"psp28i": {
"target_site": "CTGCAG",
"name": "Psp28I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas species RFL28",
"methylation_site": ""
},
"bsasi": {
"target_site": "GGNCC",
"name": "BsaSI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Bacillus stearothermophilus D47",
"methylation_site": ""
},
"bsp1286i": {
"target_site": "GDGCH^C",
"name": "Bsp1286I",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)",
"Promega Corporation (7/10)"
],
"source": "IAM 1286",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations.",
"Schildkraut, I., Christ, C., Unpublished observations.",
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476.",
"Zhu, H., Unpublished observations.",
"Zhu, Z., Unpublished observations."
],
"prototype": "SduI",
"organism": "Bacillus sphaericus",
"methylation_site": "?(5)"
},
"pfl8i": {
"target_site": "G^GATCC",
"name": "Pfl8I",
"suppliers": [],
"source": "A.V. Pertsev",
"references": [
"Pertsev, A.V., Denmukhametov, M.M., Anoshkin, A.G., Ariskina, E.V., Berezin, I.A., Solonin, A.S., Kuzmin, N.P., (2000) Prikl. Biokhim. Mikrobiol., vol. 36, pp. 13-16."
],
"prototype": "BamHI",
"organism": "Pseudomonas fluorescens 8",
"methylation_site": ""
},
"eco200i": {
"target_site": "CCNGG",
"name": "Eco200I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Escherichia coli RFL200",
"methylation_site": ""
},
"hpyf4i": {
"target_site": "GTSAC",
"name": "HpyF4I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL4",
"methylation_site": ""
},
"rtr20i": {
"target_site": "GAAGAC",
"name": "Rtr20I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BbvII",
"organism": "Rhizobium trifolii 20",
"methylation_site": ""
},
"hhaii": {
"target_site": "G^ANTC",
"name": "HhaII",
"suppliers": [],
"source": "ATCC 10014",
"references": [
"Kelly, S., Kaddurah-Daouk, R., Smith, H.O., (1985) J. Biol. Chem., vol. 260, pp. 15339-15344.",
"Kelly, S.V., Unpublished observations.",
"Mann, M.B., Rao, R.N., Smith, H.O., (1978) Gene, vol. 3, pp. 97-112.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Haemophilus haemolyticus",
"methylation_site": "2(6)"
},
"ecl2zi": {
"target_site": "CTGCA^G",
"name": "Ecl2zI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "PstI",
"organism": "Enterobacter cloacae 2z",
"methylation_site": ""
},
"nfliii": {
"target_site": "?",
"name": "NflIII",
"suppliers": [],
"source": "NRCC 31011",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria flavescens",
"methylation_site": ""
},
"bspci": {
"target_site": "CGAT^CG",
"name": "BspCI",
"suppliers": [],
"source": "T.G. Simcox",
"references": [
"Nelson, M., McClelland, M., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Bacillus species C1",
"methylation_site": ""
},
"llaei": {
"target_site": "?",
"name": "LlaEI",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Nyengaard, N., Vogensen, F.K., Josephsen, J., (1995) Gene, vol. 157, pp. 13-18."
],
"prototype": "",
"organism": "Lactococcus lactis W12",
"methylation_site": ""
},
"kpn49kii": {
"target_site": "^CCSGG",
"name": "Kpn49kII",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravetz, A.N., Zakharova, M.V., Beljetzkaja, I.V., Pertzev, A.V., Spivak, O.I., Solonin, A.S., (1993) Nucleic Acids Res., vol. 21, pp. 1501."
],
"prototype": "CauII",
"organism": "Klebsiella pneumoniae 49k",
"methylation_site": ""
},
"eco1342i": {
"target_site": "AACNNNNNNGTGC",
"name": "Eco1342I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoKI",
"organism": "Escherichia coli EC1342",
"methylation_site": ""
},
"cfr58i": {
"target_site": "CCWGG",
"name": "Cfr58I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL58",
"methylation_site": ""
},
"bsp49i": {
"target_site": "GATC",
"name": "Bsp49I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL49",
"methylation_site": ""
},
"cvioi": {
"target_site": "RGCY",
"name": "CviOI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Agarwal, K., Unpublished observations."
],
"prototype": "CviJI",
"organism": "Chlorella strain NC64A (IL-3D)",
"methylation_site": ""
},
"hpy303i": {
"target_site": "GTAC",
"name": "Hpy303I",
"suppliers": [],
"source": "N.R. Salama",
"references": [
"Humbert, O., Salama, N.R., (2008) Nucleic Acids Res., vol. 36, pp. 6893-6906.",
"Salama, N.R., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori 30-3",
"methylation_site": ""
},
"apei": {
"target_site": "ACGCGT",
"name": "ApeI",
"suppliers": [],
"source": "ATCC 15445",
"references": [
"Hall, D., Morgan, R., Unpublished observations."
],
"prototype": "MluI",
"organism": "Achromobacter pestifer",
"methylation_site": ""
},
"eco31i": {
"target_site": "GGTCTC(1/5)",
"name": "Eco31I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Bitinaite, J., Maneliene, Z., Menkevicius, S., Klimasauskas, S., Butkus, V., Janulaitis, A., (1992) Nucleic Acids Res., vol. 20, pp. 4981-4985.",
"Bitinaite, J., Mitkaite, G., Dauksaite, V., Jakubauskas, A., Timinskas, A., Vaisvila, R., Lubys, A., Janulaitis, A., (2002) Mol. Genet. Genomics, vol. 267, pp. 664-672.",
"Butkus, V., Bitinaite, J., Kersulyte, D., Janulaitis, A., (1985) Biochim. Biophys. Acta, vol. 826, pp. 208-212."
],
"prototype": "",
"organism": "Escherichia coli RFL31",
"methylation_site": "4(5)"
},
"bsphi": {
"target_site": "T^CATGA",
"name": "BspHI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 394",
"references": [
"Hall, D., Camp, R., Morgan, R., Hoffman, L., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species H",
"methylation_site": "?(6)"
},
"eco113i": {
"target_site": "GRGCYC",
"name": "Eco113I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL113",
"methylation_site": ""
},
"pxymzi": {
"target_site": "CCTNAGG",
"name": "PxyMZI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Mrazek, J., Piknova, M., Pristas, P., Kopecny, J., (2005) Anaerobe, vol. 11, pp. 280-284."
],
"prototype": "SauI",
"organism": "Pseudobutyrivibrio species MZ 5",
"methylation_site": ""
},
"pae39i": {
"target_site": "CTGCAG",
"name": "Pae39I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL39",
"methylation_site": ""
},
"rspxi": {
"target_site": "T^CATGA",
"name": "RspXI",
"suppliers": [],
"source": "W.-C. Tsui",
"references": [
"Tsui, W.-C., Elgar, G., Merrill, C., Maunders, M., (1988) Nucleic Acids Res., vol. 16, pp. 4178."
],
"prototype": "BspHI",
"organism": "Rhodococcus species",
"methylation_site": ""
},
"bspjii": {
"target_site": "AT^CGAT",
"name": "BspJII",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Anikeitcheva, N.V., Samko, O.T., Kalugin, A.A., Fitsner, A.B., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species",
"methylation_site": ""
},
"m.dcaii": {
"target_site": "?",
"name": "M.DcaII",
"suppliers": [],
"source": "R. Cella",
"references": [
"Bernacchia, G., Primo, A., Giorgetti, L., Pitto, L., Cella, R., (1998) Plant J., vol. 13, pp. 317-329."
],
"prototype": "",
"organism": "Daucus carota",
"methylation_site": "?(5)"
},
"phabi": {
"target_site": "?",
"name": "PhaBI",
"suppliers": [],
"source": "P.E. Shewen",
"references": [
"Ryan, K.A., Lo, Y.C., (1999) Nucleic Acids Res., vol. 27, pp. 1505-1511."
],
"prototype": "",
"organism": "Pasteurella haemolytica",
"methylation_site": "?(6)"
},
"vha1168i": {
"target_site": "GGCC",
"name": "Vha1168I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Vibrio harveyi 1168",
"methylation_site": ""
},
"bsami": {
"target_site": "GAATGC(1/-1)",
"name": "BsaMI",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Bacillus stearothermophilus M293",
"methylation_site": ""
},
"eco61i": {
"target_site": "CCWGG",
"name": "Eco61I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL61",
"methylation_site": ""
},
"bstli": {
"target_site": "CTCGAG",
"name": "BstLI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus stearothermophilus L95",
"methylation_site": ""
},
"ccini": {
"target_site": "GC^GGCCGC",
"name": "CciNI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Rechkunova, N.I., Prihodko, E.A., Kileva, E.V., Kusner, Y.S., Verchozina, V.A., Degtyarev, S.Kh., (1995) Gene, vol. 157, pp. 99-100."
],
"prototype": "NotI",
"organism": "Curtobacterium citreum N",
"methylation_site": ""
},
"bsodi": {
"target_site": "CGGCCG",
"name": "BsoDI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "XmaIII",
"organism": "Bacillus stearothermophilus SE2091",
"methylation_site": ""
},
"uba1144i": {
"target_site": "ATCGAT",
"name": "Uba1144I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1144",
"methylation_site": ""
},
"mlii": {
"target_site": "GGWCC",
"name": "MliI",
"suppliers": [],
"source": "K.O. Stetter",
"references": [
"Frey, G., Thomm, M., Frey, B., Schmitz, G.G., Jarsch, M., Kessler, C., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Methanoplanus limicola",
"methylation_site": ""
},
"hpyf7i": {
"target_site": "CTNAG",
"name": "HpyF7I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL7",
"methylation_site": ""
},
"hali": {
"target_site": "G^AATTC",
"name": "HalI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Verhosina, V.A., Vinogradova, T.P., (1990) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 1, pp. 35-37."
],
"prototype": "EcoRI",
"organism": "Hafnia alvei B6",
"methylation_site": ""
},
"bst1274i": {
"target_site": "GATC",
"name": "Bst1274I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus 1274",
"methylation_site": ""
},
"msp199i": {
"target_site": "CCGG",
"name": "Msp199I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "HpaII",
"organism": "Microcystis species 199",
"methylation_site": ""
},
"splaiii": {
"target_site": "CGATCG",
"name": "SplAIII",
"suppliers": [],
"source": "UTEX 2340",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "PvuI",
"organism": "Spirulina platensis",
"methylation_site": ""
},
"m.cviaiv": {
"target_site": "RGCB",
"name": "M.CviAIV",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Kutish, G.F., Li, Y., Lu, Z., Furuta, M., Rock, D.L., Van Etten, J.L., (1996) Virology, vol. 223, pp. 303-317.",
"Zhang, Y., Nelson, M., Van Etten, J.L., (1992) Nucleic Acids Res., vol. 20, pp. 1637-1642."
],
"prototype": "M.CviJI",
"organism": "Chlorella strain NC64A (PBCV-1)",
"methylation_site": "3(5)"
},
"aceiii": {
"target_site": "CAGCTC(7/11)",
"name": "AceIII",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Affram, C., Rahman, H., Unpublished observations."
],
"prototype": "",
"organism": "Anabaena cedrorum",
"methylation_site": ""
},
"asp10hii": {
"target_site": "CCANNNN^NTGG",
"name": "Asp10HII",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Ito, T., Kotani, H., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "PflMI",
"organism": "Acidiphilium species 10H",
"methylation_site": ""
},
"cthii": {
"target_site": "CC^WGG",
"name": "CthII",
"suppliers": [],
"source": "ATCC 27405",
"references": [
"Choi, K.D., Yoo, O.J., (1990) Korean Biochem. J., vol. 23, pp. 418-421."
],
"prototype": "EcoRII",
"organism": "Clostridium thermocellum",
"methylation_site": ""
},
"bspm90i": {
"target_site": "GTA^TAC",
"name": "BspM90I",
"suppliers": [],
"source": "V.M. Kramarov",
"references": [
"Kramarov, V.M., Unpublished observations."
],
"prototype": "SnaI",
"organism": "Bacillus species M90",
"methylation_site": ""
},
"nbai": {
"target_site": "GCCGGC",
"name": "NbaI",
"suppliers": [],
"source": "ATCC 19296",
"references": [
"Qiang, B.-Q., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Nocardia brasiliensis",
"methylation_site": ""
},
"hpyf36i": {
"target_site": "GTSAC",
"name": "HpyF36I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL36",
"methylation_site": ""
},
"nami": {
"target_site": "GGCGCC",
"name": "NamI",
"suppliers": [],
"source": "ATCC 27809",
"references": [
"Lin, P.-M., Roberts, R.J., Unpublished observations."
],
"prototype": "NarI",
"organism": "Nocardia amarae",
"methylation_site": ""
},
"mhoi": {
"target_site": "GGNCC",
"name": "MhoI",
"suppliers": [],
"source": "A. Duarte",
"references": [
"Paquete, C.M.V., Duarte, A., Vitor, J.M.B., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Mycoplasma hominis 2297",
"methylation_site": ""
},
"bspa2i": {
"target_site": "C^CTAGG",
"name": "BspA2I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Shinkarenko, N.M., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvrII",
"organism": "Bacillus species A2",
"methylation_site": ""
},
"bspli": {
"target_site": "GGN^NCC",
"name": "BspLI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Lazareviciute, L., Petrusyte, M., Maneliene, Z., Trinkunaite, L., Capskaja, L., Kiuduliene, E., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "NlaIV",
"organism": "Bacillus species RJ3-212",
"methylation_site": ""
},
"acaii": {
"target_site": "GGATCC",
"name": "AcaII",
"suppliers": [],
"source": "CCAP 1403/1",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Anabaena catenula",
"methylation_site": ""
},
"olii": {
"target_site": "CACNN^NNGTG",
"name": "OliI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Padegimiene, E., Maneliene, Z., Petrusyte, M., Janulaitis, A., (2001) Nucleic Acids Res., vol. 29."
],
"prototype": "",
"organism": "Oceanospirillum linum 4-5D",
"methylation_site": ""
},
"nspbi": {
"target_site": "TTCGAA",
"name": "NspBI",
"suppliers": [],
"source": "ATCC 29131",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AsuII",
"organism": "Nostoc species",
"methylation_site": ""
},
"ahai": {
"target_site": "CC^SGG",
"name": "AhaI",
"suppliers": [],
"source": "ATCC 29534",
"references": [
"Whitehead, P.R., Brown, N.L., (1985) Arch. Microbiol., vol. 141, pp. 70-74."
],
"prototype": "CauII",
"organism": "Aphanothece halophytica",
"methylation_site": ""
},
"uba1095i": {
"target_site": "CCGCGG",
"name": "Uba1095I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1095",
"methylation_site": ""
},
"sexci": {
"target_site": "CCGC^GG",
"name": "SexCI",
"suppliers": [],
"source": "M. Bae",
"references": [
"Bae, M., Par, J.-O., Hwang, H.-Y., Yim, J., (1993) Korean J. Microbiol., vol. 31, pp. 544-549.",
"Bae, M., Suh, W.-N., Park, J.-O., Kim, H.T., Lee, K.-J., (1993) Korean J. Microbiol., vol. 31, pp. 465-470."
],
"prototype": "SacII",
"organism": "Streptomyces exfoliatus",
"methylation_site": ""
},
"koxi": {
"target_site": "GGTNACC",
"name": "KoxI",
"suppliers": [],
"source": "CAMB 2553",
"references": [
"Sohail, A., Khan, E., Mushtaq, R., Riazuddin, S., Unpublished observations."
],
"prototype": "BstEII",
"organism": "Klebsiella oxytoca",
"methylation_site": ""
},
"eco53ki": {
"target_site": "GAG^CTC",
"name": "Eco53kI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "A.S. Solonin",
"references": [
"Chan, S.-H., Unpublished observations.",
"Denjmukhametov, M.M., Zakharova, M.V., Kravets, A.N., Pertsev, A.V., Sineva, E.V., Repik, A.V., Beletskaya, I.V., Gromova, E.S., Solonin, A.S., (1997) Mol. Biol. (Mosk), vol. 31, pp. 831-838."
],
"prototype": "SacI",
"organism": "Escherichia coli 53k",
"methylation_site": ""
},
"uba1163i": {
"target_site": "GGATCC",
"name": "Uba1163I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1163",
"methylation_site": ""
},
"styski": {
"target_site": "CGATNNNNNNNGTTA",
"name": "StySKI",
"suppliers": [],
"source": "L.R. Bullas",
"references": [
"Thorpe, P.H., Ternent, D., Murray, N.E., (1997) Nucleic Acids Res., vol. 25, pp. 1694-1700."
],
"prototype": "",
"organism": "Salmonella kaduna CDC-388",
"methylation_site": "?(6)"
},
"esp12i": {
"target_site": "GGYRCC",
"name": "Esp12I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL12",
"methylation_site": ""
},
"bscpi": {
"target_site": "CTNAG",
"name": "BscPI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Bacillus species 9006",
"methylation_site": ""
},
"sgrbi": {
"target_site": "CCGC^GG",
"name": "SgrBI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "V. Bouriotis",
"references": [
"Rina, M., Karagouni, A., Pagomenou, M., Bouriotis, V., (1991) Nucleic Acids Res., vol. 19, pp. 6342."
],
"prototype": "SacII",
"organism": "Streptomyces griseus",
"methylation_site": ""
},
"nassi": {
"target_site": "GAGCTC",
"name": "NasSI",
"suppliers": [],
"source": "ATCC 9969",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations."
],
"prototype": "SacI",
"organism": "Nocardia asteroides",
"methylation_site": ""
},
"eco158ii": {
"target_site": "TACGTA",
"name": "Eco158II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "SnaBI",
"organism": "Escherichia coli RFL158",
"methylation_site": ""
},
"ckri": {
"target_site": "?",
"name": "CkrI",
"suppliers": [],
"source": "DSM 44385",
"references": [
"Luyten, Y.A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Corynebacterium kroppenstedtii",
"methylation_site": "?(6)"
},
"uba1214i": {
"target_site": "GGCC",
"name": "Uba1214I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1214",
"methylation_site": ""
},
"bscui": {
"target_site": "GCATC",
"name": "BscUI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Bacillus species SA506a",
"methylation_site": ""
},
"hgicii": {
"target_site": "G^GWCC",
"name": "HgiCII",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Erdmann, D., (1991) Ph.D. Thesis, Justus-Liebig University, Geissen, W. Germany, pp. 1-204.",
"Erdmann, D., Horst, G., Dusterhoft, A., Kroger, M., (1992) Gene, vol. 117, pp. 15-22.",
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141."
],
"prototype": "AvaII",
"organism": "Herpetosiphon giganteus Hpg9",
"methylation_site": "?(5)"
},
"nmeci": {
"target_site": "^GATC",
"name": "NmeCI",
"suppliers": [],
"source": "C.A. Hart",
"references": [
"Sullivan, K.M., Macdonald, H.J., Saunders, J.R., (1987) FEMS Microbiol. Lett., vol. 44, pp. 389-393."
],
"prototype": "MboI",
"organism": "Neisseria meningitidis C114",
"methylation_site": ""
},
"ecor10i": {
"target_site": "?",
"name": "EcoR10I",
"suppliers": [],
"source": "ATCC 35329",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR10",
"methylation_site": "?(6)"
},
"bspche15i": {
"target_site": "GGCC",
"name": "BspCHE15I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus CHE 15S",
"methylation_site": ""
},
"bseii": {
"target_site": "GTTAAC",
"name": "BseII",
"suppliers": [],
"source": "T. Oshima",
"references": [
"Shinomiya, T., Unpublished observations."
],
"prototype": "HpaI",
"organism": "Bacillus stearothermophilus strain 822",
"methylation_site": ""
},
"bsp13i": {
"target_site": "T^CCGGA",
"name": "Bsp13I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Bacillus species 13",
"methylation_site": ""
},
"kpn14i": {
"target_site": "CCWGG",
"name": "Kpn14I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Klebsiella pneumoniae RFL14",
"methylation_site": ""
},
"cvirii": {
"target_site": "G^TAC",
"name": "CviRII",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Nelson, M., Zhang, Y., Van Etten, J.L., (1993) DNA Methylation: Molecular Biology and Biological Significance, ed. Jost, J.P., Saluz, H.P. (Birkhauser Verlag, Basel, Switzerland), vol. 0, pp. 186-211."
],
"prototype": "RsaI",
"organism": "Chlorella strain NC64A (XZ-6E)",
"methylation_site": "3(6)"
},
"fspmsi": {
"target_site": "G^GWCC",
"name": "FspMSI",
"suppliers": [],
"source": "M. Sargent",
"references": [
"Walker, J.N.B., Dean, P.G., Saunders, J.R., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Fischerella species",
"methylation_site": ""
},
"alwi": {
"target_site": "GGATC(4/5)",
"name": "AlwI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 402",
"references": [
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R., Bonventre, J., Unpublished observations.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "BinI",
"organism": "Acinetobacter lwoffi",
"methylation_site": "3(6),-2(6)"
},
"bpli": {
"target_site": "(8/13)GAGNNNNNCTC(13/8)",
"name": "BplI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Maneliene, Z., Petrusyte, M., Janulaitis, A., (1997) Nucleic Acids Res., vol. 25, pp. 4444-4446."
],
"prototype": "",
"organism": "Bacillus pumilus",
"methylation_site": ""
},
"eco232i": {
"target_site": "GRGCYC",
"name": "Eco232I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL232",
"methylation_site": ""
},
"ssl16219i": {
"target_site": "?",
"name": "Ssl16219I",
"suppliers": [],
"source": "FERM 16219",
"references": [
"Tomita, M., Toyama, K., Hiramatsu, A., Sho, K., Takahashi, T., Japanese Patent Office, 2000."
],
"prototype": "",
"organism": "Streptococcus salivarius subsp. thermophilus",
"methylation_site": ""
},
"ecl133i": {
"target_site": "CTGCAG",
"name": "Ecl133I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "PstI",
"organism": "Enterobacter cloacae RFL133",
"methylation_site": ""
},
"plaai": {
"target_site": "C^YCGRG",
"name": "PlaAI",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Waleron, K., Swiatek, W., Biedrzycka, I., Podhajska, A.J., (2001) FEMS Microbiol. Lett., vol. 198, pp. 135-140."
],
"prototype": "AvaI",
"organism": "Phormidium laminosum",
"methylation_site": ""
},
"hpyf48ii": {
"target_site": "ACNGT",
"name": "HpyF48II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL48",
"methylation_site": ""
},
"m.cviav": {
"target_site": "?",
"name": "M.CviAV",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Lu, Z., Li, Y., Que, Q., Kutish, G.F., Rock, D.L., Van Etten, J.L., (1996) Virology, vol. 216, pp. 102-123.",
"Nelson, M., Burbank, D.E., Van Etten, J.L., (1998) Biol. Chem., vol. 379, pp. 423-428."
],
"prototype": "",
"organism": "Chlorella strain NC64A (PBCV-1)",
"methylation_site": "?(5)"
},
"shy1766i": {
"target_site": "CTCGAG",
"name": "Shy1766I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces hygroscopicus 1766",
"methylation_site": ""
},
"nphi": {
"target_site": "^GATC",
"name": "NphI",
"suppliers": [],
"source": "C.A. Hart",
"references": [
"Sullivan, K.M., Macdonald, H.J., Saunders, J.R., (1987) FEMS Microbiol. Lett., vol. 44, pp. 389-393."
],
"prototype": "MboI",
"organism": "Neisseria pharyngis C245",
"methylation_site": ""
},
"bsp28i": {
"target_site": "CTGGAG",
"name": "Bsp28I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "GsuI",
"organism": "Bacillus species RFL28",
"methylation_site": ""
},
"bbii": {
"target_site": "CTGCAG",
"name": "BbiI",
"suppliers": [],
"source": "T. Khosaka",
"references": [
"Khosaka, T., Kiwaki, M., (1984) FEBS Lett., vol. 177, pp. 57-60."
],
"prototype": "PstI",
"organism": "Bifidobacterium bifidum YIT4007",
"methylation_site": ""
},
"r1.ssu2479i": {
"target_site": "GATC",
"name": "R1.Ssu2479I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis strain 89-2479",
"methylation_site": ""
},
"sboi": {
"target_site": "CCGCGG",
"name": "SboI",
"suppliers": [],
"source": "ATCC 3310",
"references": [
"Shimotsu, H., Takahashi, H., Saito, H., (1980) Agric. Biol. Chem., vol. 44, pp. 1665-1666.",
"Takahashi, H., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces bobili",
"methylation_site": ""
},
"bspkt6i": {
"target_site": "GAT^C",
"name": "BspKT6I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Shapovalova, N.I., Zheleznaja, L.A., Matvienko, N.I., (1993) Nucleic Acids Res., vol. 21, pp. 5794.",
"Shapovalova, N.I., Zheleznaya, L.A., Matvienko, N.I., (1994) Biokhimiia, vol. 59, pp. 1730-1738.",
"Zhu, Z., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species KT6",
"methylation_site": "2(6)"
},
"bst44i": {
"target_site": "?",
"name": "Bst44I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kramarov, V.M., Fomenkov, A.I., Matvienko, N.I., (1988) Bioorg. Khim., vol. 14, pp. 916-920."
],
"prototype": "",
"organism": "Bacillus stearothermophilus 4.4",
"methylation_site": ""
},
"uba1207i": {
"target_site": "GGCC",
"name": "Uba1207I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1207",
"methylation_site": ""
},
"esp23i": {
"target_site": "CGTCTC",
"name": "Esp23I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Esp3I",
"organism": "Erwinia species RFL23",
"methylation_site": ""
},
"afl83ii": {
"target_site": "GGCC",
"name": "Afl83II",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "HaeIII",
"organism": "Anabaena flos-aquae NIVA-CYA83",
"methylation_site": ""
},
"mroni": {
"target_site": "G^CCGGC",
"name": "MroNI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Belichenko, O.A., Prikhodko, E.A., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Micrococcus roseus N",
"methylation_site": ""
},
"lwei": {
"target_site": "GCATC(5/9)",
"name": "LweI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Zutautiene, D., Maneliene, Z., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Listeria welshimeri RFL 131",
"methylation_site": ""
},
"hgiai": {
"target_site": "GWGCW^C",
"name": "HgiAI",
"suppliers": [],
"source": "J.H. Parish",
"references": [
"Brown, N.L., McClelland, M., Whitehead, P.R., (1980) Gene, vol. 9, pp. 49-68.",
"Lunnen, K.D., Wilson, G.G., US Patent Office, 1991."
],
"prototype": "",
"organism": "Herpetosiphon giganteus HP1023",
"methylation_site": ""
},
"plei": {
"target_site": "GAGTC(4/5)",
"name": "PleI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 418",
"references": [
"Kong, H., Higgins, L., Dalton, M., Kucera, R., Schildkraut, I., Wilson, G., European Patent Office, 2007.",
"Kong, H., Higgins, L.S., US Patent Office, 2002.",
"Morgan, R., Stote, R., Soltis, A., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas lemoignei",
"methylation_site": ""
},
"hpyf64iv": {
"target_site": "CGCG",
"name": "HpyF64IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Helicobacter pylori RFL64",
"methylation_site": ""
},
"llagi": {
"target_site": "CTNGAYG",
"name": "LlaGI",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Madsen, A., Josephsen, J., (2001) FEMS Microbiol. Lett., vol. 200, pp. 91-96.",
"Smith, R.M., Diffin, F.M., Savery, N.J., Josephsen, J., Szczelkun, M.D., (2009) Nucleic Acids Res., vol. 37, pp. 7206-7218."
],
"prototype": "",
"organism": "Lactococcus lactis",
"methylation_site": "-6(6)"
},
"uba87i": {
"target_site": "GGTACC",
"name": "Uba87I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Unidentified bacterium RFL87",
"methylation_site": ""
},
"tmu1i": {
"target_site": "CCSGG",
"name": "Tmu1I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "CauII",
"organism": "Tuberoidobacter mutans RFL1",
"methylation_site": ""
},
"ecor42i": {
"target_site": "?",
"name": "EcoR42I",
"suppliers": [],
"source": "ATCC 35361",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR42",
"methylation_site": "?(6)"
},
"bsedi": {
"target_site": "C^CNNGG",
"name": "BseDI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations.",
"Vilkaitis, G., Janulaitis, A., Unpublished observations."
],
"prototype": "SecI",
"organism": "Bacillus stearothermophilus RFL1434",
"methylation_site": ""
},
"xmaiii": {
"target_site": "C^GGCCG",
"name": "XmaIII",
"suppliers": [],
"source": "ATCC 9924",
"references": [
"Kunkel, L.M., Silberklang, M., McCarthy, B.J., (1979) J. Mol. Biol., vol. 132, pp. 133-139.",
"Trautner, T.A., Unpublished observations."
],
"prototype": "",
"organism": "Xanthomonas malvacearum",
"methylation_site": "4(5)"
},
"vpak3ai": {
"target_site": "CACGTG",
"name": "VpaK3AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "PmaCI",
"organism": "Vibrio parahaemolyticus Nakatsugawa",
"methylation_site": ""
},
"psc2i": {
"target_site": "GAANNNNTTC",
"name": "Psc2I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "XmnI",
"organism": "Planktothrix species 2",
"methylation_site": ""
},
"kpn378i": {
"target_site": "CCGC^GG",
"name": "Kpn378I",
"suppliers": [],
"source": "Y.P. Zernov",
"references": [
"Zernov, Y.P., Lebedev, L.R., Babkin, I.V., Chizhikov, V.E., (1990) Bioorg. Khim., vol. 16, pp. 603-604."
],
"prototype": "SacII",
"organism": "Klebsiella pneumoniae 378",
"methylation_site": ""
},
"r2.ssu4961i": {
"target_site": "GATC",
"name": "R2.Ssu4961I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis strain 4961",
"methylation_site": ""
},
"pae43i": {
"target_site": "CCGCGG",
"name": "Pae43I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Pseudomonas aeruginosa RFL43",
"methylation_site": ""
},
"hpyf64ii": {
"target_site": "ACNGT",
"name": "HpyF64II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL64",
"methylation_site": ""
},
"omiai": {
"target_site": "GRGCYC",
"name": "OmiAI",
"suppliers": [],
"source": "NEB 970",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Oceanospirillum minutulum",
"methylation_site": ""
},
"rlu4i": {
"target_site": "GGATCC",
"name": "Rlu4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Rhodococcus luteus RFL4",
"methylation_site": ""
},
"eco82i": {
"target_site": "GAATTC",
"name": "Eco82I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Escherichia coli RFL82",
"methylation_site": ""
},
"bsp6ii": {
"target_site": "CTGAAG",
"name": "Bsp6II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco57I",
"organism": "Bacillus species RFL6",
"methylation_site": ""
},
"uba1433i": {
"target_site": "AGCT",
"name": "Uba1433I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AluI",
"organism": "Unidentified bacterium RFL1433",
"methylation_site": ""
},
"bsp139i": {
"target_site": "CTCGAG",
"name": "Bsp139I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus species RFL139",
"methylation_site": ""
},
"bsplu11iii": {
"target_site": "GGGAC(10/14)",
"name": "BspLU11III",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Chernov, A.V., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (1994) Biokhimiia, vol. 59, pp. 1714-1729.",
"Lepikhov, K., Tchernov, A., Zheleznaja, L., Matvienko, N., Walter, J., Trautner, T.A., (2001) Nucleic Acids Res., vol. 29, pp. 4691-4698."
],
"prototype": "FinI",
"organism": "Bacillus species LU11",
"methylation_site": "4(6)"
},
"bsp55i": {
"target_site": "CCSGG",
"name": "Bsp55I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "CauII",
"organism": "Bacillus species RFL55",
"methylation_site": ""
},
"eco137ki": {
"target_site": "^CCNGG",
"name": "Eco137kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Denjmukhametov, M.M., Zakharova, M.V., Kravets, A.N., Pertsev, A.V., Sineva, E.V., Repik, A.V., Beletskaya, I.V., Gromova, E.S., Solonin, A.S., (1997) Mol. Biol. (Mosk), vol. 31, pp. 831-838."
],
"prototype": "ScrFI",
"organism": "Escherichia coli 137k",
"methylation_site": ""
},
"afei": {
"target_site": "AGC^GCT",
"name": "AfeI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 1775",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations.",
"Lunnen, K., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "Eco47III",
"organism": "Alcaligenes faecalis T2774",
"methylation_site": ""
},
"psp5ii": {
"target_site": "RG^GWCCY",
"name": "Psp5II",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations.",
"Janulaitis, A., Petrusyte, M., Maneliene, Z., Capskaya, L., Kiuduliene, L., Butkus, V., Unpublished observations."
],
"prototype": "PpuMI",
"organism": "Pseudomonas species RFL5",
"methylation_site": ""
},
"bstz13i": {
"target_site": "?",
"name": "BstZ13I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus 36A",
"methylation_site": ""
},
"sspm2i": {
"target_site": "TACGTA",
"name": "SspM2I",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "SnaBI",
"organism": "Spirulina species M2",
"methylation_site": ""
},
"clti": {
"target_site": "GG^CC",
"name": "CltI",
"suppliers": [],
"source": "DSM 484",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Caryophanon latum",
"methylation_site": ""
},
"ahyi": {
"target_site": "C^CCGGG",
"name": "AhyI",
"suppliers": [],
"source": "NEB 503",
"references": [
"Tenkanen, T., Unpublished observations."
],
"prototype": "SmaI",
"organism": "Aeromonas hydrophila",
"methylation_site": ""
},
"bse17i": {
"target_site": "CC^WGG",
"name": "Bse17I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus species 17",
"methylation_site": ""
},
"sgrti": {
"target_site": "CCDS(10/14)",
"name": "SgrTI",
"suppliers": [],
"source": "M. Fischbach",
"references": [
"Cohen-Karni, D., Zheng, Y., Unpublished observations.",
"Fischbach, M. et al., Unpublished observations."
],
"prototype": "",
"organism": "Streptomyces griseoflavus Tu4000",
"methylation_site": ""
},
"rrh4273i": {
"target_site": "G^TCGAC",
"name": "Rrh4273I",
"suppliers": [],
"source": "ATCC 4273",
"references": [
"Yebra, M.J., Novella, I.S., Barbes, C., Aparicio, J.F., Martin, C.G., Hardisson, C., Sanchez, J., (1991) J. Gen. Microbiol., vol. 137, pp. 1279-1284."
],
"prototype": "SalI",
"organism": "Rhodococcus rhodochrous",
"methylation_site": "5(6)"
},
"uba1314i": {
"target_site": "GGWCC",
"name": "Uba1314I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1314",
"methylation_site": ""
},
"bstt7i": {
"target_site": "T^GATCA",
"name": "BstT7I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "BclI",
"organism": "Bacillus stearothermophilus T7",
"methylation_site": ""
},
"ecl1zi": {
"target_site": "CTGCAG",
"name": "Ecl1zI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "PstI",
"organism": "Enterobacter cloacae 1z",
"methylation_site": ""
},
"vpak65i": {
"target_site": "GGWCC",
"name": "VpaK65I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Kotani, H., Mise, K., (1992) Gene, vol. 117, pp. 103-106."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus AQ3206",
"methylation_site": ""
},
"pmai": {
"target_site": "CTGCAG",
"name": "PmaI",
"suppliers": [],
"source": "D.G. Comb",
"references": [
"Schildkraut, I., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas maltophilia",
"methylation_site": ""
},
"bsu54i": {
"target_site": "G^GNCC",
"name": "Bsu54I",
"suppliers": [],
"source": "V.M. Kramarov",
"references": [
"Kramarov, V.M., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Bacillus subtilis 54",
"methylation_site": ""
},
"hpyf44iii": {
"target_site": "TG^CA",
"name": "HpyF44III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori RFL44",
"methylation_site": ""
},
"ssti": {
"target_site": "GAGCT^C",
"name": "SstI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)"
],
"source": "S. Goff",
"references": [
"Goff, S.P., Rambach, A., (1978) Gene, vol. 3, pp. 347-352.",
"Longo, M.C., Smith, M.D., Harris, R.D., International Patent Office, 1996.",
"Muller, F., Stoffel, S., Clarkson, S.G., Unpublished observations."
],
"prototype": "SacI",
"organism": "Streptomyces stanford",
"methylation_site": ""
},
"bsppi": {
"target_site": "GGATC(4/5)",
"name": "BspPI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Padegimiene, E., Maneliene, Z., Norgeliene, D., Vonseviciene, E., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BinI",
"organism": "Bacillus species d 1-34",
"methylation_site": ""
},
"bsp100i": {
"target_site": "GGWCC",
"name": "Bsp100I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus species RFL100",
"methylation_site": ""
},
"drdiii": {
"target_site": "CGATCG",
"name": "DrdIII",
"suppliers": [],
"source": "NEB 479",
"references": [
"Polisson, C., Murray, R.G.E., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Deinococcus radiodurans",
"methylation_site": ""
},
"uba1338i": {
"target_site": "CCGG",
"name": "Uba1338I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Unidentified bacterium RFL1338",
"methylation_site": ""
},
"hpyf13i": {
"target_site": "GTSAC",
"name": "HpyF13I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL13",
"methylation_site": ""
},
"vcho2i": {
"target_site": "GAATTC",
"name": "VchO2I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "EcoRI",
"organism": "Vibrio cholerae NCTC4711",
"methylation_site": ""
},
"eagi": {
"target_site": "C^GGCCG",
"name": "EagI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 368",
"references": [
"Brooks, J.E., Sznyter, L.A., US Patent Office, 1991.",
"Morgan, R., Camp, R., Soltis, A., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Samuelson, J., Unpublished observations.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "XmaIII",
"organism": "Enterobacter agglomerans",
"methylation_site": "4(5)"
},
"bsub763i": {
"target_site": "GGATCC",
"name": "BsuB763I",
"suppliers": [],
"source": "Y.I. Buryanov",
"references": [
"Bogdarina, I.G., Nadirova, I.M., Buryanov, Y.I., (1986) Mikrobiologiia, vol. 55, pp. 699-700."
],
"prototype": "BamHI",
"organism": "Bacillus subtilis B763",
"methylation_site": ""
},
"sol3335i": {
"target_site": "CAGCTG",
"name": "Sol3335I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Leung, S.M., Chan, K.Y., Suen, Y.K., Shaw, P.C., (1989) Nucleic Acids Res., vol. 17, pp. 10133."
],
"prototype": "PvuII",
"organism": "Streptomyces olivaceus IMRU 3335",
"methylation_site": ""
},
"sauhi": {
"target_site": "CCTNAGG",
"name": "SauHI",
"suppliers": [],
"source": "J. Muchova",
"references": [
"Muchova, J., Lacova, B., Godany, A., Sevcikova, B., (1991) J. Basic Microbiol., vol. 31, pp. 141-147."
],
"prototype": "SauI",
"organism": "Streptomyces aureofaciens 13",
"methylation_site": ""
},
"mspni": {
"target_site": "?",
"name": "MspNI",
"suppliers": [],
"source": "P. Sharma",
"references": [
"Sharma, P., Unpublished observations."
],
"prototype": "",
"organism": "Meiothermus species Nubra I",
"methylation_site": ""
},
"cfr4i": {
"target_site": "GGNCC",
"name": "Cfr4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "AsuI",
"organism": "Citrobacter freundii RFL4",
"methylation_site": ""
},
"bsrbri": {
"target_site": "GATNN^NNATC",
"name": "BsrBRI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsaBI",
"organism": "Bacillus stearothermophilus BR",
"methylation_site": ""
},
"thai": {
"target_site": "CG^CG",
"name": "ThaI",
"suppliers": [],
"source": "ATCC 25905",
"references": [
"Beletskaya, I.V., Zakharova, M.V., Shlyapnikov, M.G., Semenova, L.M., Solonin, A.S., (2000) Nucleic Acids Res., vol. 28, pp. 3817-3822.",
"McConnell, D.J., Searcy, D.G., Sutcliffe, J.G., (1978) Nucleic Acids Res., vol. 5, pp. 1729-1739.",
"Prangishvili, D.A., Chinchaladze, D.Z., Chelidze, M.G., (1987) Biotekhnologiya, vol. 3, pp. 440-446.",
"Ruepp, A., Graml, W., Santos-Martinez, M.-L., Koretke, K.K., Volker, C., Mewes, H.W., Frishman, D., Stocker, S., Lupas, A.N., Baumeister, W., (2000) Nature, vol. 407, pp. 508-513."
],
"prototype": "FnuDII",
"organism": "Thermoplasma acidophilum",
"methylation_site": "?(4)"
},
"bpti": {
"target_site": "CC^WGG",
"name": "BptI",
"suppliers": [],
"source": "P. Babu",
"references": [
"Suma, M.A., Janaki, B., Babu, P., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus pantothenticus",
"methylation_site": ""
},
"psc49i": {
"target_site": "?",
"name": "Psc49I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species 49",
"methylation_site": ""
},
"hpyf26ii": {
"target_site": "GGCC",
"name": "HpyF26II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL26",
"methylation_site": ""
},
"seqai": {
"target_site": "?",
"name": "SeqAI",
"suppliers": [],
"source": "N. Anand",
"references": [
"Elango, K., Reddy, S.S., MohanRaj, R., Anand, N., Unpublished observations."
],
"prototype": "",
"organism": "Synechocystis equalis",
"methylation_site": ""
},
"uba90i": {
"target_site": "CCGCGG",
"name": "Uba90I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL90",
"methylation_site": ""
},
"apyi": {
"target_site": "CC^WGG",
"name": "ApyI",
"suppliers": [],
"source": "ATCC 49829",
"references": [
"DiLauro, R., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Arthrobacter pyridinolis",
"methylation_site": ""
},
"mel5ti": {
"target_site": "GATC",
"name": "Mel5TI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2004) FEMS Microbiol. Lett., vol. 236, pp. 91-95.",
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193."
],
"prototype": "MboI",
"organism": "Megasphaera elsedenii 5T",
"methylation_site": ""
},
"bhaii": {
"target_site": "GGCC",
"name": "BhaII",
"suppliers": [],
"source": "JCM 9153",
"references": [
"Morgan, R.D., Unpublished observations.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Roberts, R.J., Unpublished observations.",
"Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N., Kuhara, S., Horikoshi, K., (2000) Nucleic Acids Res., vol. 28, pp. 4317-4331."
],
"prototype": "HaeIII",
"organism": "Bacillus halodurans C-125",
"methylation_site": "?(5)"
},
"bsiqi": {
"target_site": "T^GATCA",
"name": "BsiQI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "BclI",
"organism": "Bacillus species",
"methylation_site": ""
},
"bsxi": {
"target_site": "ACTGGG",
"name": "BsxI",
"suppliers": [],
"source": "NEB 1400",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "BfiI",
"organism": "Bacillus simplex 2472",
"methylation_site": ""
},
"uba76i": {
"target_site": "GGTACC",
"name": "Uba76I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Unidentified bacterium RFL76",
"methylation_site": ""
},
"nspsai": {
"target_site": "C^YCGRG",
"name": "NspSAI",
"suppliers": [],
"source": "D. Jones",
"references": [
"Dean, P.D.C., Walker, J.N.B., (1985) Biochem. Soc. Trans., vol. 13, pp. 1055-1058."
],
"prototype": "AvaI",
"organism": "Nostoc species SA",
"methylation_site": ""
},
"hphi": {
"target_site": "GGTGA(8/7)",
"name": "HphI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"New England Biolabs (1/11)"
],
"source": "ATCC 49700",
"references": [
"Daniels, J.S., Gates, K.S., (1994) ACS Abstracts, vol. 208, pp. 66.",
"Kleid, D., Humayun, Z., Jeffrey, A., Ptashne, M., (1976) Proc. Natl. Acad. Sci. U. S. A., vol. 73, pp. 293-297.",
"Lubys, A., Lubiene, J., Kulakauskas, S., Stankevicius, K., Timinskas, A., Janulaitis, A., (1996) Nucleic Acids Res., vol. 24, pp. 2760-2766.",
"Middleton, J.H., Stankus, P.V., Edgell, M.H., Hutchison, C.A. III, Unpublished observations.",
"Nelson, M., Unpublished observations."
],
"prototype": "",
"organism": "Haemophilus parahaemolyticus",
"methylation_site": "-2(5)"
},
"psppri": {
"target_site": "CCYCAG(15/13)",
"name": "PspPRI",
"suppliers": [],
"source": "J.M. Tiedje",
"references": [
"Copeland, A., Lucas, S., Lapidus, A., Barry, K., Detter, J.C., Glavina del Rio, T., Hammon, N., Israni, S., Dalin, E., Tice, H., Bruce, D., Pitluck, S., Richardson, P., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Psychrobacter sp. PRwf-1",
"methylation_site": "?(6)"
},
"ngowi": {
"target_site": "RGCGCY",
"name": "NgoWI",
"suppliers": [],
"source": "G.A. Wilson",
"references": [
"Wilson, G.A., Young, F.E., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Neisseria gonorrhoeae",
"methylation_site": ""
},
"bavi": {
"target_site": "CAG^CTG",
"name": "BavI",
"suppliers": [],
"source": "ATCC 6348",
"references": [
"McEvoy, S., Roberts, R.J., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Bacillus alvei",
"methylation_site": ""
},
"bthci": {
"target_site": "GCNG^C",
"name": "BthCI",
"suppliers": [],
"source": "NEB 1402",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "Fnu4HI",
"organism": "Bacillus thuringiensis 2294",
"methylation_site": "?(5)"
},
"pbujki": {
"target_site": "GGATG",
"name": "PbuJKI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Mrazek, J., Piknova, M., Pristas, P., Kopecny, J., (2005) Anaerobe, vol. 11, pp. 280-284."
],
"prototype": "FokI",
"organism": "Pseudobutyrivibrio species JK 720",
"methylation_site": ""
},
"ntasi": {
"target_site": "AGGCCT",
"name": "NtaSI",
"suppliers": [],
"source": "ATCC 31190",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations."
],
"prototype": "StuI",
"organism": "Nocardia tartaricans",
"methylation_site": ""
},
"bstrz246i": {
"target_site": "ATTT^AAAT",
"name": "BstRZ246I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "SwaI",
"organism": "Bacillus stearothermophilus RZ246",
"methylation_site": ""
},
"ppu1253i": {
"target_site": "GACGTC",
"name": "Ppu1253I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Dauksaite, V., Janulaitis, A., Unpublished observations.",
"Janulaitis, A., Unpublished observations."
],
"prototype": "AatII",
"organism": "Pseudomonas putida RFL1253",
"methylation_site": ""
},
"hpyf11i": {
"target_site": "CTNAG",
"name": "HpyF11I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL11",
"methylation_site": ""
},
"bsc107i": {
"target_site": "CCNNNNN^NNGG",
"name": "Bsc107I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BsiYI",
"organism": "Bacillus schlegelii 107",
"methylation_site": ""
},
"rshii": {
"target_site": "CCSGG",
"name": "RshII",
"suppliers": [],
"source": "S. Kaplan",
"references": [
"Kramarov, V.M., Pachkunov, D.M., Matvienko, N.I., (1983) Nek. Aspekty Fiziol. Mikroorg. Akad. Nauk. SSR., ed. Gaziev, A.I. (Nauchn. Tsentr Biol. Issled., Pushchino, USSR.), vol. 0, pp. 22-26."
],
"prototype": "CauII",
"organism": "Rhodobacter sphaeroides 2.4.1",
"methylation_site": ""
},
"csp68ki": {
"target_site": "G^GWCC",
"name": "Csp68KI",
"suppliers": [],
"source": "ATCC 51142",
"references": [
"Soper, B.W., Reddy, K.J., (1994) J. Bacteriol., vol. 176, pp. 5565-5570.",
"Welsh, E.A., Liberton, M., Stockel, J., Sato, H., Loh, T., Wang, C., Wollam, A., Fulton, R.S., Clifton, S.W., Jacobs, J.M., Aurora, R., Sherman, L.A., Smith, R.D., Wilson, R.K., Pakrasi, H.B., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Cyanothece species BH68K",
"methylation_site": "?(5)"
},
"uba1428i": {
"target_site": "CCWGG",
"name": "Uba1428I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1428",
"methylation_site": ""
},
"vcho90i": {
"target_site": "GGNCC",
"name": "VchO90I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "AsuI",
"organism": "Vibrio cholerae 1457-78",
"methylation_site": ""
},
"slai": {
"target_site": "C^TCGAG",
"name": "SlaI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "ATCC 8664",
"references": [
"Takahashi, H., Shimizu, M., Saito, H., Ikeda, Y., Sugisaki, H., (1979) Gene, vol. 5, pp. 9-18."
],
"prototype": "XhoI",
"organism": "Streptomyces lavendulae",
"methylation_site": ""
},
"erpi": {
"target_site": "G^GWCC",
"name": "ErpI",
"suppliers": [],
"source": "D. Jones",
"references": [
"Walker, J.N.B., Dean, P.G., Saunders, J.R., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Erwinia rhaponici",
"methylation_site": ""
},
"rlu1i": {
"target_site": "GATC",
"name": "Rlu1I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Rhodococcus luteus RFL1",
"methylation_site": ""
},
"sflhk7462i": {
"target_site": "CCWGG",
"name": "SflHK7462I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 7462",
"methylation_site": ""
},
"sfoi": {
"target_site": "GGC^GCC",
"name": "SfoI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 369",
"references": [
"Krotee, S., Ganatra, M., Grandoni, R., Unpublished observations.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "NarI",
"organism": "Serratia fonticola",
"methylation_site": "?(5)"
},
"uba1184ii": {
"target_site": "CCTNAGG",
"name": "Uba1184II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SauI",
"organism": "Unidentified bacterium RFL1184",
"methylation_site": ""
},
"avrbi": {
"target_site": "GGCC",
"name": "AvrBI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Calmero, J., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Arthrobacter variabilis",
"methylation_site": ""
},
"cspai": {
"target_site": "A^CCGGT",
"name": "CspAI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "N.N. Sokolov",
"references": [
"Rina, M., Markaki, M., Sokolov, N., Bouriotis, V., Unpublished observations."
],
"prototype": "AgeI",
"organism": "Corynebacterium species 301",
"methylation_site": ""
},
"uba1180i": {
"target_site": "AGGCCT",
"name": "Uba1180I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1180",
"methylation_site": ""
},
"hpyf25ii": {
"target_site": "GTSAC",
"name": "HpyF25II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL25",
"methylation_site": ""
},
"stri": {
"target_site": "C^TCGAG",
"name": "StrI",
"suppliers": [
"Bangalore Genei (1/11)"
],
"source": "V. Nagaraja",
"references": [
"Chandrashekaran, S., Shankar, A.B., Babu, P., Paul, B.D., Nagaraja, V., (1999) Curr. Sci., vol. 77, pp. 273-276."
],
"prototype": "XhoI",
"organism": "Streptomyces thermodiastaticus",
"methylation_site": ""
},
"cfli": {
"target_site": "CTGCA^G",
"name": "CflI",
"suppliers": [],
"source": "IFO 3753",
"references": [
"Hiraoka, N., Kita, K., Nakajima, H., Kimizuka, F., Obayashi, A., (1985) J. Ferment. Technol., vol. 63, pp. 151-157."
],
"prototype": "PstI",
"organism": "Cellulomonas flavigena",
"methylation_site": ""
},
"umi7i": {
"target_site": "TGATCA",
"name": "Umi7I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "BclI",
"organism": "Unidentified bacterium 7T",
"methylation_site": ""
},
"uba1170i": {
"target_site": "AGGCCT",
"name": "Uba1170I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1170",
"methylation_site": ""
},
"rma517i": {
"target_site": "CTAG",
"name": "Rma517I",
"suppliers": [],
"source": "ITI 517",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"noti": {
"target_site": "GC^GGCCGC",
"name": "NotI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 14630",
"references": [
"Borsetti, R., Wise, D., Qiang, B.-Q., Schildkraut, I., Unpublished observations.",
"Morgan, R.D., Benner, J.S., Claus, T.E., US Patent Office, 1994.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Qiang, B.-Q., Schildkraut, I., (1987) Methods Enzymol., vol. 155, pp. 15-21."
],
"prototype": "",
"organism": "Nocardia otitidis-caviarum",
"methylation_site": "?(4)"
},
"atuai": {
"target_site": "?",
"name": "AtuAI",
"suppliers": [],
"source": "ATCC 15955",
"references": [
"Sciaky, D., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Agrobacterium tumefaciens",
"methylation_site": ""
},
"rma515i": {
"target_site": "CTAG",
"name": "Rma515I",
"suppliers": [],
"source": "ITI 515",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"bsp92i": {
"target_site": "CTCGAG",
"name": "Bsp92I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus species RFL92",
"methylation_site": ""
},
"esphk30i": {
"target_site": "CCWGG",
"name": "EspHK30I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Enterobacter species N30",
"methylation_site": ""
},
"pmaci": {
"target_site": "CAC^GTG",
"name": "PmaCI",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "C.A. Hart",
"references": [
"Walker, J.N.B., Dean, P.D.G., Saunders, J.R., (1986) Nucleic Acids Res., vol. 14, pp. 1293-1301."
],
"prototype": "",
"organism": "Pseudomonas maltophilia CB50P",
"methylation_site": ""
},
"sthgi": {
"target_site": "GGTACC",
"name": "SthGI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY217",
"methylation_site": ""
},
"hpych4vi": {
"target_site": "TCNNGA",
"name": "HpyCH4VI",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "Hpy178III",
"organism": "Helicobacter pylori CH4",
"methylation_site": ""
},
"bsp2500i": {
"target_site": "GGCC",
"name": "Bsp2500I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus 2500",
"methylation_site": ""
},
"beti": {
"target_site": "W^CCGGW",
"name": "BetI",
"suppliers": [],
"source": "NEB 678",
"references": [
"Zhou, B., Morgan, R., Unpublished observations."
],
"prototype": "",
"organism": "Brevibacterium acetyliticum",
"methylation_site": ""
},
"bstfni": {
"target_site": "CG^CG",
"name": "BstFNI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Nayakshina, T.N., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Bacillus stearothermophilus",
"methylation_site": ""
},
"uba1370i": {
"target_site": "CCSGG",
"name": "Uba1370I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1370",
"methylation_site": ""
},
"sali": {
"target_site": "G^TCGAC",
"name": "SalI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 49789",
"references": [
"Alvarez, M.A., Chater, K.F., Rosario, M.R., (1993) Mol. Microbiol., vol. 8, pp. 243-252.",
"Arrand, J.R., Myers, P.A., Roberts, R.J., (1978) J. Mol. Biol., vol. 118, pp. 127-135.",
"Rodicio, M.R., Quinton-Jager, T., Moran, L.S., Slatko, B.E., Wilson, G.G., (1994) Gene, vol. 151, pp. 167-172."
],
"prototype": "",
"organism": "Streptomyces albus G",
"methylation_site": "5(6)"
},
"ecai": {
"target_site": "G^GTNACC",
"name": "EcaI",
"suppliers": [],
"source": "DSM 30056",
"references": [
"Brenner, V., Venetianer, P., Kiss, A., (1990) Nucleic Acids Res., vol. 18, pp. 355-359.",
"Hobom, G., Schwarz, E., Melzer, M., Mayer, H., (1981) Nucleic Acids Res., vol. 9, pp. 4823-4832.",
"Szilak, L., Venetianer, P., Kiss, A., (1992) Eur. J. Biochem., vol. 209, pp. 391-397."
],
"prototype": "BstEII",
"organism": "Enterobacter cloacae",
"methylation_site": "5(6)"
},
"pae24i": {
"target_site": "CTGCAG",
"name": "Pae24I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL24",
"methylation_site": ""
},
"tsp507i": {
"target_site": "TCCGGA",
"name": "Tsp507I",
"suppliers": [],
"source": "ITI 203",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Thermus species",
"methylation_site": ""
},
"bpusi": {
"target_site": "GGGAC(10/14)",
"name": "BpuSI",
"suppliers": [],
"source": "NEB 1539",
"references": [
"Xu, D., Zhu, Z., Xu, S.-Y., Unpublished observations.",
"Zhu, Z., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus pumilus",
"methylation_site": "?(6)"
},
"r2.llaji": {
"target_site": "?",
"name": "R2.LlaJI",
"suppliers": [],
"source": "G.F. Fitzgerald",
"references": [
"O'Driscoll, J., Fitzgerald, G.F., van Sinderen, D., (2005) Mol. Microbiol., vol. 57, pp. 1532-1544.",
"O'Driscoll, J., Glynn, F., Cahalane, O., O'Connell-Motherway, M., Fitzgerald, G.F., van Sinderen, D., (2004) Appl. Environ. Microbiol., vol. 70, pp. 5546-5556."
],
"prototype": "",
"organism": "Lactococcus lactis",
"methylation_site": "3(5)"
},
"npehkvvi": {
"target_site": "?",
"name": "NpeHKVVI",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Nodularia species HKVV",
"methylation_site": ""
},
"rpabi": {
"target_site": "CCCGCAG(20/18)",
"name": "RpaBI",
"suppliers": [],
"source": "ATCC BAA-1124",
"references": [
"Fomenkov, A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Rhodopseudomonas palustris BisB18",
"methylation_site": "?(6)"
},
"bsp228i": {
"target_site": "TCCGGA",
"name": "Bsp228I",
"suppliers": [],
"source": "ITI 228",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Bacillus species",
"methylation_site": ""
},
"cfr10i": {
"target_site": "R^CCGGY",
"name": "Cfr10I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Grazulis, S., Deibert, M., Rimseliene, R., Skirgaila, R., Sasnauskas, G., Lagunavicius, A., Repin, V., Urbanke, C., Huber, R., Siksnys, V., (2002) Nucleic Acids Res., vol. 30, pp. 876-885.",
"Janulaitis, A., Stakenas, P.S., Berlin, Y.A., (1983) FEBS Lett., vol. 161, pp. 210-212.",
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129.",
"Klimasauskas, S., Butkus, V., Janulaitis, A., (1987) Mol. Biol. (Mosk), vol. 21, pp. 87-92."
],
"prototype": "",
"organism": "Citrobacter freundii RFL10",
"methylation_site": "2(5)"
},
"sau32i": {
"target_site": "GGNCC",
"name": "Sau32I",
"suppliers": [],
"source": "I. Mikula",
"references": [
"Godany, A., Bukovska, G., Farkasovska, J., Brnakova, Z., Dmitriev, A., Tkacikova, L., Ayele, T., Mikula, I., (2004) Folia Microbiol. (Praha), vol. 49, pp. 307-314."
],
"prototype": "AsuI",
"organism": "Staphylococcus aureus 32",
"methylation_site": ""
},
"bspsi": {
"target_site": "CCWGG",
"name": "BspSI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "EcoRII",
"organism": "Bacillus species S",
"methylation_site": ""
},
"otui": {
"target_site": "AGCT",
"name": "OtuI",
"suppliers": [],
"source": "ATCC 27403",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "AluI",
"organism": "Oerskovia turbata",
"methylation_site": ""
},
"pfei": {
"target_site": "G^AWTC",
"name": "PfeI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Zakareviciene, L., Unpublished observations.",
"Markauskiene, J., Capskaja, L., Kiuduliene, L., Petrusyte, M., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "TfiI",
"organism": "Psychrobacter faecalis RFL1",
"methylation_site": ""
},
"ali2882i": {
"target_site": "CTGCAG",
"name": "Ali2882I",
"suppliers": [],
"source": "K. Komagata",
"references": [
"Yamada, Y., Sasaki, J., (1984) J. Gen. Appl. Microbiol., vol. 30, pp. 309-312."
],
"prototype": "PstI",
"organism": "Acetobacter liquefaciens",
"methylation_site": ""
},
"badi": {
"target_site": "CTCGAG",
"name": "BadI",
"suppliers": [],
"source": "G.N. Gabrichevsky",
"references": [
"Skrypina, N.A., Kramarov, V.M., Lyannaya, A.M., Smolyaninov, V.V., Goncharova, G.I., (1988) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 15-16."
],
"prototype": "XhoI",
"organism": "Bifidobacterium adolescentis LVA1",
"methylation_site": ""
},
"saufi": {
"target_site": "GATC",
"name": "SauFI",
"suppliers": [],
"source": "E.E. Stobberingh",
"references": [
"Stobberingh, E.E., Schiphof, R., Sussenbach, J.S., (1977) J. Bacteriol., vol. 131, pp. 645-649."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus 879",
"methylation_site": ""
},
"bsmyi": {
"target_site": "CCNNNNNNNGG",
"name": "BsmYI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "BsiYI",
"organism": "Bacillus stearothermophilus Y36",
"methylation_site": ""
},
"phaai": {
"target_site": "?",
"name": "PhaAI",
"suppliers": [],
"source": "S.K. Highlander",
"references": [
"Highlander, S.K., Garza, O., (1996) Gene, vol. 178, pp. 89-96."
],
"prototype": "",
"organism": "Pasteurella haemolytica",
"methylation_site": "?(6)"
},
"bsabi": {
"target_site": "GATNN^NNATC",
"name": "BsaBI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 537",
"references": [
"Chen, Z., Kong, H., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus B674",
"methylation_site": ""
},
"bstoi": {
"target_site": "CC^WGG",
"name": "BstOI",
"suppliers": [
"Promega Corporation (7/10)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus O22",
"methylation_site": ""
},
"bstai": {
"target_site": "?",
"name": "BstAI",
"suppliers": [],
"source": "A. Atkinson",
"references": [
"Bingham, A.H.A., Sharp, R.J., Atkinson, T., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus 240",
"methylation_site": ""
},
"csibi": {
"target_site": "GC^GGCCGC",
"name": "CsiBI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., (1995) Vestn. Akad. Med. Nauk SSSR, vol. 2, pp. 47-51."
],
"prototype": "NotI",
"organism": "Corynebacterium species B",
"methylation_site": ""
},
"eco25i": {
"target_site": "GRGCYC",
"name": "Eco25I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL25",
"methylation_site": ""
},
"apeki": {
"target_site": "G^CWGC",
"name": "ApeKI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "ATCC 700893",
"references": [
"Kawarabayasi, Y. et al., (1999) DNA Res., vol. 6, pp. 83-101.",
"Opitz, L., Xu, S.-Y., Unpublished observations.",
"Xu, S.-Y., Opitz, L., Unpublished observations."
],
"prototype": "TseI",
"organism": "Aeropyrum pernix K1",
"methylation_site": "?(5)"
},
"uba1145i": {
"target_site": "ATCGAT",
"name": "Uba1145I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1145",
"methylation_site": ""
},
"bsoei": {
"target_site": "CCTNNNNNAGG",
"name": "BsoEI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "EcoNI",
"organism": "Bacillus stearothermophilus OC391",
"methylation_site": ""
},
"bco118i": {
"target_site": "R^CCGGY",
"name": "Bco118I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "Cfr10I",
"organism": "Bacillus coagulans 118",
"methylation_site": ""
},
"haci": {
"target_site": "^GATC",
"name": "HacI",
"suppliers": [],
"source": "IAM 12094",
"references": [
"Hiraoka, N., Kita, K., Nakajima, H., Kimizuka, F., Obayashi, A., (1985) J. Ferment. Technol., vol. 63, pp. 151-157."
],
"prototype": "MboI",
"organism": "Halococcus acetoinfaciens",
"methylation_site": ""
},
"bscji": {
"target_site": "CCANNNNNNTGG",
"name": "BscJI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "BstXI",
"organism": "Bacillus species 9A",
"methylation_site": ""
},
"mboii": {
"target_site": "GAAGA(8/7)",
"name": "MboII",
"suppliers": [
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 10900",
"references": [
"Brown, N.L., Hutchison, C.A. III, Smith, M., (1980) J. Mol. Biol., vol. 140, pp. 143-148.",
"Endow, S.A., (1977) J. Mol. Biol., vol. 114, pp. 441-449.",
"Gelinas, R.E., Myers, P.A., Roberts, R.J., (1977) J. Mol. Biol., vol. 114, pp. 169-179.",
"McClelland, M., Nelson, M., Cantor, C.R., (1985) Nucleic Acids Res., vol. 13, pp. 7171-7182."
],
"prototype": "",
"organism": "Moraxella bovis",
"methylation_site": "5(6)"
},
"m.ecoec67dam": {
"target_site": "GATC",
"name": "M.EcoEc67Dam",
"suppliers": [],
"source": "M. Inouye",
"references": [
"Hsu, M.-Y., Inouye, M., Inouye, S., (1990) Proc. Natl. Acad. Sci. U. S. A., vol. 87, pp. 9454-9458."
],
"prototype": "MboI",
"organism": "Escherichia coli",
"methylation_site": "2(6)"
},
"apoi": {
"target_site": "R^AATTY",
"name": "ApoI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 723",
"references": [
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Polisson, C., Robinson, D., (1992) Nucleic Acids Res., vol. 20, pp. 2888."
],
"prototype": "",
"organism": "Arthrobacter protophormiae",
"methylation_site": "?(6)"
},
"ecl699ki": {
"target_site": "CTGCAG",
"name": "Ecl699kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "PstI",
"organism": "Enterobacter cloacae 699k",
"methylation_site": ""
},
"sbai": {
"target_site": "CAGCTG",
"name": "SbaI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Matsui, M., Mise, K., Yoshida, Y., Ishidate, M., (1986) Eisei Shikenjo Hokoku, vol. 104, pp. 92-96.",
"Mise, K., Unpublished observations.",
"Naderer, M., Brust, J.R., Knowle, D., Blumenthal, R.M., (2002) J. Bacteriol., vol. 184, pp. 2411-2419."
],
"prototype": "PvuII",
"organism": "Salmonella bareilly",
"methylation_site": ""
},
"bsh108ai": {
"target_site": "ATCGAT",
"name": "Bsh108AI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "ClaI",
"organism": "Bacillus sphaericus 108A",
"methylation_site": ""
},
"eco203i": {
"target_site": "GGTCTC",
"name": "Eco203I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL203",
"methylation_site": ""
},
"bsmgii": {
"target_site": "AAGCTT",
"name": "BsmGII",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Bacillus stearothermophilus G170",
"methylation_site": ""
},
"bbruii": {
"target_site": "GTCGAC",
"name": "BbrUII",
"suppliers": [],
"source": "D. van Sinderen",
"references": [
"O'Connell-Motherway, M., Fitzgerald, G.F., van Sinderen, D., (2009) Micro. Biotech., vol. 2, pp. 321-332."
],
"prototype": "SalI",
"organism": "Bifidobacterium breve UCC2003",
"methylation_site": "?(5)"
},
"hpyf40iii": {
"target_site": "GTSAC",
"name": "HpyF40III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL40",
"methylation_site": ""
},
"hpyf37i": {
"target_site": "CTNAG",
"name": "HpyF37I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL37",
"methylation_site": ""
},
"acyii": {
"target_site": "?",
"name": "AcyII",
"suppliers": [],
"source": "CCAP 1403/2a",
"references": [
"de Waard, A., Korsuize, J., van Beveren, C.P., Maat, J., (1978) FEBS Lett., vol. 96, pp. 106-110."
],
"prototype": "",
"organism": "Anabaena cylindrica",
"methylation_site": ""
},
"hinsafi": {
"target_site": "AAGCTT",
"name": "HinSAFI",
"suppliers": [],
"source": "A. Bakir",
"references": [
"Putrus, S.G., Al-Ani, F., Bakir, A., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Haemophilus influenzae serotype b, SAF6",
"methylation_site": ""
},
"nspei": {
"target_site": "CYCGRG",
"name": "NspEI",
"suppliers": [],
"source": "ATCC 27896",
"references": [
"Flores, E., Wolk, C.P., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Nostoc species",
"methylation_site": ""
},
"koyi": {
"target_site": "GTCGAC",
"name": "KoyI",
"suppliers": [],
"source": "ATCC 13183",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "SalI",
"organism": "Klebsiella oxytoca",
"methylation_site": ""
},
"pspxi": {
"target_site": "VC^TCGAGB",
"name": "PspXI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 1782",
"references": [
"Gonchar, D.A., Abdurashitov, M.A., Belichenko, O.A., Dedkov, V.S., Mezentseva, N.V., Tomilova, J.E., Degtyarev, S.K., (2005) Ovchinnikov Bull. Biotechnol. Phys. Chem. Biol., vol. 1, pp. 18-23.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas species A1-1",
"methylation_site": ""
},
"sfr303i": {
"target_site": "CCGC^GG",
"name": "Sfr303I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces fradiae 303",
"methylation_site": ""
},
"nlaci": {
"target_site": "CATCAC(19/17)",
"name": "NlaCI",
"suppliers": [],
"source": "R. Chalmers",
"references": [
"Chalmers, R., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Neisseria lactamica 020-06",
"methylation_site": "?(6)"
},
"bse23i": {
"target_site": "CCNNNNNNNGG",
"name": "Bse23I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D., Tereschenko, T., Chizikov, V.E., Andreeva, I., (1995) Gene, vol. 157, pp. 321-322.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BsiYI",
"organism": "Bacillus species 23",
"methylation_site": ""
},
"lpni": {
"target_site": "RGC^GCY",
"name": "LpnI",
"suppliers": [],
"source": "A. Brown",
"references": [
"Chen, G.C.C., Brown, A., Lema, M.W., (1986) Can. J. Microbiol., vol. 32, pp. 591-593.",
"Hamablet, L., Chen, G.C., Brown, A., Roberts, R.J., (1989) Nucleic Acids Res., vol. 17, pp. 6417."
],
"prototype": "HaeII",
"organism": "Legionella pneumophila Philadelphia 1",
"methylation_site": ""
},
"uba1284i": {
"target_site": "GCTNAGC",
"name": "Uba1284I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EspI",
"organism": "Unidentified bacterium RFL1284",
"methylation_site": ""
},
"eco49i": {
"target_site": "CTGCAG",
"name": "Eco49I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Escherichia coli RFL49",
"methylation_site": ""
},
"hin3i": {
"target_site": "CCSGG",
"name": "Hin3I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "CauII",
"organism": "Haemophilus influenzae RFL3",
"methylation_site": ""
},
"cfr57i": {
"target_site": "TCCGGA",
"name": "Cfr57I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Citrobacter freundii RFL57",
"methylation_site": ""
},
"eco130i": {
"target_site": "C^CWWGG",
"name": "Eco130I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Gilvonauskaite, R., Padegimiene, A., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations.",
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "StyI",
"organism": "Escherichia coli RFL130",
"methylation_site": ""
},
"pflfi": {
"target_site": "GACN^NNGTC",
"name": "PflFI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 999",
"references": [
"Nkenfou, C., Polisson, C., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "Tth111I",
"organism": "Pseudomonas fluorescens biotype F",
"methylation_site": ""
},
"aacli": {
"target_site": "GGATCC",
"name": "AacLI",
"suppliers": [],
"source": "IFO 12388",
"references": [
"Seurinck, J., van Montagu, M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Acetobacter aceti sub. liquefaciens",
"methylation_site": ""
},
"vhai": {
"target_site": "GGCC",
"name": "VhaI",
"suppliers": [],
"source": "ATCC 14126",
"references": [
"Hurlin, P., Schildkraut, I., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Vibrio harveyi",
"methylation_site": ""
},
"sfr382i": {
"target_site": "CCGCGG",
"name": "Sfr382I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces fradiae 382",
"methylation_site": ""
},
"uba1331i": {
"target_site": "CTTAAG",
"name": "Uba1331I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AflII",
"organism": "Unidentified bacterium RFL1331",
"methylation_site": ""
},
"hpyf68i": {
"target_site": "ACNGT",
"name": "HpyF68I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL68",
"methylation_site": ""
},
"bmcai": {
"target_site": "AGT^ACT",
"name": "BmcAI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 181",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Bacillus macroides",
"methylation_site": ""
},
"bco163i": {
"target_site": "CTRYAG",
"name": "Bco163I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "SfeI",
"organism": "Bacillus coagulans 163",
"methylation_site": ""
},
"bsp129i": {
"target_site": "CTCGAG",
"name": "Bsp129I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Bacillus species RFL129",
"methylation_site": ""
},
"nunii": {
"target_site": "GG^CGCC",
"name": "NunII",
"suppliers": [],
"source": "ATCC 21806",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "NarI",
"organism": "Nocardia uniformis",
"methylation_site": ""
},
"sna3286i": {
"target_site": "TCGCGA",
"name": "Sna3286I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "NruI",
"organism": "Sphaerotilus natans 3286",
"methylation_site": ""
},
"uba1152i": {
"target_site": "GGCC",
"name": "Uba1152I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1152",
"methylation_site": ""
},
"ecl57ki": {
"target_site": "CCWGG",
"name": "Ecl57kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae 57k",
"methylation_site": ""
},
"rsani": {
"target_site": "G^TAC",
"name": "RsaNI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Nayakshina, T.V., Boltengagen, A.A., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Rhodopseudomonas sphaeroides N",
"methylation_site": ""
},
"yenci": {
"target_site": "CTGCAG",
"name": "YenCI",
"suppliers": [],
"source": "T. Maruyama",
"references": [
"Miyahara, M., Maruyama, T., Wake, A., Mise, K., (1988) Appl. Environ. Microbiol., vol. 54, pp. 577-580."
],
"prototype": "PstI",
"organism": "Yersinia enterocolitica 08 Bi3995",
"methylation_site": ""
},
"uba1444i": {
"target_site": "CTGGAG",
"name": "Uba1444I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "GsuI",
"organism": "Unidentified bacterium RFL1444",
"methylation_site": ""
},
"xmai": {
"target_site": "C^CCGGG",
"name": "XmaI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "ATCC 9924",
"references": [
"Endow, S.A., Roberts, R.J., (1977) J. Mol. Biol., vol. 112, pp. 521-529.",
"Lunnen, K.D., Wilson, G.G., US Patent Office, 1991.",
"Lunnen, K.D., Wilson, G.G., European Patent Office, 1998."
],
"prototype": "SmaI",
"organism": "Xanthomonas malvacearum",
"methylation_site": "?(4)"
},
"eco178i": {
"target_site": "GATATC",
"name": "Eco178I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Escherichia coli RFL178",
"methylation_site": ""
},
"uban13i": {
"target_site": "CCGG",
"name": "UbaN13I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Gomez-Rodriguez, J.A., Huete-Perez, J.A., (2008) Encuentro, vol. 15, pp. 70-87."
],
"prototype": "HpaII",
"organism": "Unidentified bacterium 1ATMS-3, Nicaragua",
"methylation_site": ""
},
"hpyf59i": {
"target_site": "CTNAG",
"name": "HpyF59I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL59",
"methylation_site": ""
},
"uba1114i": {
"target_site": "CCWGG",
"name": "Uba1114I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1114",
"methylation_site": ""
},
"asp47i": {
"target_site": "CTCGAG",
"name": "Asp47I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Alcaligenes species 47",
"methylation_site": ""
},
"avci": {
"target_site": "G^GNCC",
"name": "AvcI",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Rutkowska, S.M., Skowron, P.M., Podhajska, A.J., (1995) Gene, vol. 157, pp. 317-318."
],
"prototype": "AsuI",
"organism": "Actinomyces violaceoniger cristalomycini",
"methylation_site": ""
},
"pac1110ii": {
"target_site": "GATATC",
"name": "Pac1110II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Pseudomonas alcaligenes RFL1110",
"methylation_site": ""
},
"bco116i": {
"target_site": "CTCTTC(1/4)",
"name": "Bco116I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Chizhikov, V.E., Tereshchenko, T.A., Lebedev, L.R., (1993) Bioorg. Khim., vol. 4, pp. 410-413.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "Ksp632I",
"organism": "Bacillus coagulans 116",
"methylation_site": ""
},
"asni": {
"target_site": "AT^TAAT",
"name": "AsnI",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Rexer, B.U., Jarsch, M., Sagmeister, C., Gluck, B., Berger, G., Kessler, C., (1988) FEBS Lett., vol. 235, pp. 241-246."
],
"prototype": "VspI",
"organism": "Arthrobacter species strain N-CM",
"methylation_site": ""
},
"pae181i": {
"target_site": "CCSGG",
"name": "Pae181I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "CauII",
"organism": "Pseudomonas aeruginosa RFL181",
"methylation_site": ""
},
"bstjz301i": {
"target_site": "C^TNAG",
"name": "BstJZ301I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Bacillus stearothermophilus JZ301",
"methylation_site": ""
},
"hpyf20i": {
"target_site": "ACNGT",
"name": "HpyF20I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL20",
"methylation_site": ""
},
"m.ggadnmt1": {
"target_site": "?",
"name": "M.GgaDnmt1",
"suppliers": [],
"source": "S. Tajima",
"references": [
"Tajima, S., Tsuda, H., Wakabayashi, N., Asano, A., Mizuno, S., Nishimori, K., (1995) J. Biochem. (Tokyo), vol. 117, pp. 1050-1057."
],
"prototype": "",
"organism": "Gallus gallus",
"methylation_site": "?(5)"
},
"blni": {
"target_site": "C^CTAGG",
"name": "BlnI",
"suppliers": [
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"Sigma Chemical Corporation (1/11)"
],
"source": "IAM 1902",
"references": [
"Yamamoto, K., Sagawa, H., Kotani, H., Hiraoka, N., Nakamura, T., US Patent Office, 1995."
],
"prototype": "AvrII",
"organism": "Brevibacterium linens",
"methylation_site": ""
},
"espi": {
"target_site": "GC^TNAGC",
"name": "EspI",
"suppliers": [],
"source": "PCC 6906",
"references": [
"Calleja, F., Dekker, B.M.M., Coursin, T., de Waard, A., (1984) FEBS Lett., vol. 178, pp. 69-72."
],
"prototype": "",
"organism": "Eucapsis species",
"methylation_site": ""
},
"lmu60i": {
"target_site": "CC^TNAGG",
"name": "Lmu60I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "SauI",
"organism": "Listeria murrayi 60",
"methylation_site": ""
},
"eagki": {
"target_site": "CCWGG",
"name": "EagKI",
"suppliers": [],
"source": "L. Kauc",
"references": [
"Kauc, L., Leszczynska, K., (1986) Acta Microbiol. Pol., vol. 35, pp. 317-320."
],
"prototype": "EcoRII",
"organism": "Enterobacter agglomerans",
"methylation_site": ""
},
"vpakutci": {
"target_site": "?",
"name": "VpaKutCI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "",
"organism": "Vibrio parahaemolyticus TVP1615",
"methylation_site": ""
},
"vpak19ai": {
"target_site": "GGNCC",
"name": "VpaK19AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AsuI",
"organism": "Vibrio parahaemolyticus Hasegawa",
"methylation_site": ""
},
"hpyf62iii": {
"target_site": "GTSAC",
"name": "HpyF62III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL62",
"methylation_site": ""
},
"bsexi": {
"target_site": "GCAGC(8/12)",
"name": "BseXI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Grigaite, R., Maneliene, Z., Capskaja, L., Kiuduliene, E., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BbvI",
"organism": "Bacillus stearothermophilus Ra 3-212",
"methylation_site": ""
},
"becai": {
"target_site": "?",
"name": "BecAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., Korolev, S.V., Rina, M., Eldarov, M.A., Gervaziev, Y.V., Skryabin, K.G., Bouriotis, V., (1998) Mol. Gen. Mikrobiol. Virusol., vol. 9, pp. 35-38."
],
"prototype": "",
"organism": "Brevibacterium species A",
"methylation_site": ""
},
"asp700i": {
"target_site": "GAANN^NNTTC",
"name": "Asp700I",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M., Kessler, C., (1989) Nucleic Acids Res., vol. 17, pp. 8879."
],
"prototype": "XmnI",
"organism": "Achromobacter species 700",
"methylation_site": ""
},
"hgibi": {
"target_site": "G^GWCC",
"name": "HgiBI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Dusterhoft, A., Erdmann, D., Kroger, M., (1991) Nucleic Acids Res., vol. 19, pp. 3207-3211.",
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141."
],
"prototype": "AvaII",
"organism": "Herpetosiphon giganteus Hpg5",
"methylation_site": "?(5)"
},
"uba1271i": {
"target_site": "CTCGAG",
"name": "Uba1271I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1271",
"methylation_site": ""
},
"bsp72i": {
"target_site": "GATC",
"name": "Bsp72I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL72",
"methylation_site": ""
},
"psp38i": {
"target_site": "CACGTG",
"name": "Psp38I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PmaCI",
"organism": "Pseudomonas species RFL38",
"methylation_site": ""
},
"bsps122i": {
"target_site": "CTGCAG",
"name": "BspS122I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "PstI",
"organism": "Bacillus species 122",
"methylation_site": ""
},
"eam1105i": {
"target_site": "GACNNN^NNGTC",
"name": "Eam1105I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Krikstulyte, V., Trinkunaite, L., Maneliene, Z., Kiuduliene, L., Petrusyte, M., Butkus, V., Unpublished observations."
],
"prototype": "",
"organism": "Enterobacter amnigenus RFL1105",
"methylation_site": ""
},
"m.cvibiii": {
"target_site": "TCGA",
"name": "M.CviBIII",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Narva, K.E., Wendell, D.L., Skrdla, M.P., Van Etten, J.L., (1987) Nucleic Acids Res., vol. 15, pp. 9807-9823."
],
"prototype": "TaqI",
"organism": "Chlorella strain NC64A (NC-1A)",
"methylation_site": "4(6)"
},
"uba1262i": {
"target_site": "CTGCAG",
"name": "Uba1262I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1262",
"methylation_site": ""
},
"ddsi": {
"target_site": "GGATCC",
"name": "DdsI",
"suppliers": [],
"source": "ATCC 27774",
"references": [
"Lucas, S. et al., Unpublished observations.",
"Makula, R.A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Desulfovibrio desulfuricans",
"methylation_site": ""
},
"mnniii": {
"target_site": "?",
"name": "MnnIII",
"suppliers": [],
"source": "ATCC 17954",
"references": [
"Hanberg, F., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Moraxella nonliquefaciens",
"methylation_site": ""
},
"bsmwi": {
"target_site": "CGTACG",
"name": "BsmWI",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "SplI",
"organism": "Bacillus stearothermophilus N658",
"methylation_site": ""
},
"nasbi": {
"target_site": "GGATCC",
"name": "NasBI",
"suppliers": [],
"source": "ATCC 9970",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Nocardia asteroides",
"methylation_site": ""
},
"acs1372i": {
"target_site": "GTCGAC",
"name": "Acs1372I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Hamafuku, H., Kotani, H., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "SalI",
"organism": "Acidobacterium capsulatum 1372",
"methylation_site": ""
},
"bstaui": {
"target_site": "T^GTACA",
"name": "BstAUI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Tomilova, J.E., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Bsp1407I",
"organism": "Bacillus stearothermophilus AU",
"methylation_site": ""
},
"bscci": {
"target_site": "GAATGC(1/-1)",
"name": "BscCI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Bacillus species 2G",
"methylation_site": ""
},
"pae40i": {
"target_site": "CTGCAG",
"name": "Pae40I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL40",
"methylation_site": ""
},
"fspii": {
"target_site": "TT^CGAA",
"name": "FspII",
"suppliers": [],
"source": "ATCC 29114",
"references": [
"Szekeres, M., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Fischerella species",
"methylation_site": ""
},
"uba1185i": {
"target_site": "CCWGG",
"name": "Uba1185I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1185",
"methylation_site": ""
},
"hinfii": {
"target_site": "AAGCTT",
"name": "HinfII",
"suppliers": [],
"source": "ATCC 17947",
"references": [
"Mann, M.B., Smith, H.O., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Haemophilus influenzae Rf",
"methylation_site": ""
},
"pspni": {
"target_site": "CTCGAG",
"name": "PspNI",
"suppliers": [],
"source": "ATCC 29862",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Pseudomonas species",
"methylation_site": ""
},
"tsui": {
"target_site": "GCGAC",
"name": "TsuI",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Riauba, L., Norgeliene, D., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Thermus scotoductus RFL2",
"methylation_site": ""
},
"xgl3217i": {
"target_site": "CGATCG",
"name": "Xgl3217I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Xanthomonas glycinae 3217",
"methylation_site": ""
},
"mgl14481i": {
"target_site": "CC^SGG",
"name": "Mgl14481I",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Waleron, K., Piechula, S., Unpublished observations."
],
"prototype": "CauII",
"organism": "Merismopedia glauca 1448-1",
"methylation_site": ""
},
"bsapi": {
"target_site": "GATC",
"name": "BsaPI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus P8",
"methylation_site": ""
},
"csii": {
"target_site": "A^CCWGGT",
"name": "CsiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G356",
"references": [
"Vitkute, J., Lapcinskaja, S., Capskaja, L., Trinkunaite, L., Zakareviciene, L., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "SexAI",
"organism": "Cellulomonas species RFL1",
"methylation_site": ""
},
"ahaiii": {
"target_site": "TTT^AAA",
"name": "AhaIII",
"suppliers": [],
"source": "ATCC 29534",
"references": [
"Whitehead, P.R., Brown, N.L., (1982) FEBS Lett., vol. 143, pp. 296-300."
],
"prototype": "",
"organism": "Aphanothece halophytica",
"methylation_site": ""
},
"hpyf3i": {
"target_site": "C^TNAG",
"name": "HpyF3I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Maneliene, Z., Unpublished observations.",
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL3",
"methylation_site": ""
},
"nspwi": {
"target_site": "GCCGGC",
"name": "NspWI",
"suppliers": [],
"source": "ATCC 29100",
"references": [
"Wickberg, L., Schildkraut, I., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Nocardia species",
"methylation_site": ""
},
"uba1244i": {
"target_site": "CCGCGG",
"name": "Uba1244I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1244",
"methylation_site": ""
},
"lladi": {
"target_site": "AGTACT",
"name": "LlaDI",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Evison, T.P., Aideh, B., Josephsen, J., Unpublished observations.",
"Nyengaard, N., Vogensen, F.K., Josephsen, J., (1995) Gene, vol. 157, pp. 13-18."
],
"prototype": "ScaI",
"organism": "Lactococcus lactis W39",
"methylation_site": ""
},
"esp4i": {
"target_site": "C^TTAAG",
"name": "Esp4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Butkus, V., Maneliene, Z., Petrusyte, M., Unpublished observations."
],
"prototype": "AflII",
"organism": "Erwinia species RFL4",
"methylation_site": ""
},
"ecoviii": {
"target_site": "A^AGCTT",
"name": "EcoVIII",
"suppliers": [],
"source": "M. Yoshikawa",
"references": [
"Mise, K., Nakajima, K., (1984) Gene, vol. 30, pp. 79-85.",
"Mruk, I., Kaczorowski, T., (2003) Appl. Environ. Microbiol., vol. 69, pp. 2638-2650."
],
"prototype": "HindIII",
"organism": "Escherichia coli E1585-68",
"methylation_site": "1(6)"
},
"psp30i": {
"target_site": "GGGCCC",
"name": "Psp30I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ApaI",
"organism": "Pseudomonas species RFL30",
"methylation_site": ""
},
"cfr59i": {
"target_site": "YGGCCR",
"name": "Cfr59I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Citrobacter freundii RFL59",
"methylation_site": ""
},
"xcai": {
"target_site": "GTA^TAC",
"name": "XcaI",
"suppliers": [],
"source": "NEB 420",
"references": [
"Morgan, R., Ellard, J., Unpublished observations."
],
"prototype": "SnaI",
"organism": "Xanthomonas campestris",
"methylation_site": ""
},
"hpyviii": {
"target_site": "CCGG",
"name": "HpyVIII",
"suppliers": [],
"source": "ATCC 49503",
"references": [
"Xu, Q., Morgan, R., Blaser, M., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Helicobacter pylori 60190",
"methylation_site": ""
},
"cvihi": {
"target_site": "GATC",
"name": "CviHI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Xia, Y., Burbank, D.E., Van Etten, J.L., Unpublished observations."
],
"prototype": "MboI",
"organism": "Chlorella strain NC64A (NC-1C)",
"methylation_site": ""
},
"eco1341i": {
"target_site": "CCANNNNNNNCTTC",
"name": "Eco1341I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "Eco646I",
"organism": "Escherichia coli EC1341",
"methylation_site": ""
},
"bst71i": {
"target_site": "GCAGC(8/12)",
"name": "Bst71I",
"suppliers": [],
"source": "Promega 71",
"references": [
"Schoenfeld, T., Fiandt, M., Schink, M., (1992) Gene, vol. 111, pp. 141-142."
],
"prototype": "BbvI",
"organism": "Bacillus stearothermophilus 71",
"methylation_site": ""
},
"xorii": {
"target_site": "CGAT^CG",
"name": "XorII",
"suppliers": [],
"source": "M. Ehrlich",
"references": [
"Choi, S.-H., (1994) Diss. Abstr., vol. 54, pp. 5462B-5463B.",
"Choi, S.H., Leach, J.E., (1994) Mol. Gen. Genet., vol. 244, pp. 383-390.",
"Gingeras, T.R., Greenough, L., Schildkraut, I., Roberts, R.J., (1981) Nucleic Acids Res., vol. 9, pp. 4525-4536.",
"Wang, R.Y.-H., Shedlarski, J.G., Farber, M.B., Kuebbing, D., Ehrlich, M., (1980) Biochim. Biophys. Acta, vol. 606, pp. 371-385."
],
"prototype": "PvuI",
"organism": "Xanthomonas oryzae",
"methylation_site": "?(5)"
},
"aoxi": {
"target_site": "^GGCC",
"name": "AoxI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Belichenko, O.A., Tarasova, G.V., Gonchar, D.A., Akishev, A.G., Dedkov, V.S., Mikhnenkova, N.A., Degtyarev, S.K., Russian Patent Office, 2010."
],
"prototype": "",
"organism": "Arthrobacter oxydans 25K",
"methylation_site": ""
},
"tmai": {
"target_site": "CGCG",
"name": "TmaI",
"suppliers": [],
"source": "K.O. Stetter",
"references": [
"Nelson, K.E. et al., (1999) Nature, vol. 399, pp. 323-329.",
"Thomm, M., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Thermotoga maritima",
"methylation_site": "?(4)"
},
"eco112i": {
"target_site": "CTGAAG",
"name": "Eco112I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Eco57I",
"organism": "Escherichia coli RFL112",
"methylation_site": ""
},
"bst100i": {
"target_site": "CC^WGG",
"name": "Bst100I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus 100",
"methylation_site": ""
},
"hpyf42iii": {
"target_site": "TCNGA",
"name": "HpyF42III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL42",
"methylation_site": ""
},
"rsr2i": {
"target_site": "CG^GWCCG",
"name": "Rsr2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shinkarenko, O.A., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "RsrII",
"organism": "Rhodobacter sphaeroides SE-I2",
"methylation_site": ""
},
"bcuai": {
"target_site": "G^GWCC",
"name": "BcuAI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Eldarov, M.A., Karpichev, I.V., Samko, O.T., Anikeitcheva, N.V., Kalugin, A.A., Khoroshoutina, E.B., Sokolov, N.N., (1992) Nucleic Acids Res., vol. 20, pp. 2898.",
"Korolev, S.V., Samko, O.T., Eldarov, M.A., Kalugin, A.A., Khoroshutina, E.B., Omelyanyuk, N.M., Sokolov, N.N., Skryabin, K.G., (1996) Bioorg. Khim., vol. 22, pp. 528-531."
],
"prototype": "AvaII",
"organism": "Bacillus cereus BKM B-814",
"methylation_site": ""
},
"bsteni": {
"target_site": "CCTNN^NNNAGG",
"name": "BstENI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "EcoNI",
"organism": "Bacillus stearothermophilus EN",
"methylation_site": ""
},
"ptai": {
"target_site": "T^CCGGA",
"name": "PtaI",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Kur, J., Bielawski, K., Podhajska, A.J., (1992) Nucleic Acids Res., vol. 20, pp. 6738."
],
"prototype": "BspMII",
"organism": "Phormidium tadzschicicum",
"methylation_site": ""
},
"senpt14bi": {
"target_site": "CCGC^GG",
"name": "SenPT14bI",
"suppliers": [],
"source": "A. Nakamura",
"references": [
"Miyahara, M., Nakamura, A., Mise, K., (1997) Biol. Pharm. Bull., vol. 20, pp. 1212-1214."
],
"prototype": "SacII",
"organism": "Salmonella enteritidis PT14b",
"methylation_site": ""
},
"xphi": {
"target_site": "CTGCAG",
"name": "XphI",
"suppliers": [],
"source": "Z.F. Bunina",
"references": [
"Bunina, Z.F., Kramarov, V.M., Smolyaninov, V.V., Tolstova, L.A., (1984) Bioorg. Khim., vol. 10, pp. 1333-1335.",
"Lai, J.Y., Yang, M.T., Unpublished observations."
],
"prototype": "PstI",
"organism": "Xanthomonas phaseoli",
"methylation_site": ""
},
"bpci": {
"target_site": "C^TRYAG",
"name": "BpcI",
"suppliers": [],
"source": "P. Babu",
"references": [
"Suma, M.A., Janaki, B., Babu, P., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"bspkmi": {
"target_site": "^GATC",
"name": "BspKMI",
"suppliers": [],
"source": "CAMB 2843",
"references": [
"Malik, K., Riazuddin, S., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species",
"methylation_site": ""
},
"hpy178iii": {
"target_site": "TC^NNGA",
"name": "Hpy178III",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Morgan, R.D., Unpublished observations.",
"Xu, Q., Blaser, M., Unpublished observations."
],
"prototype": "",
"organism": "Helicobacter pylori J178",
"methylation_site": ""
},
"scoi": {
"target_site": "GAGCTC",
"name": "ScoI",
"suppliers": [],
"source": "ATCC 10147",
"references": [
"Stote, R., Morgan, R., Unpublished observations."
],
"prototype": "SacI",
"organism": "Streptomyces coelicolor",
"methylation_site": ""
},
"bse3di": {
"target_site": "GCAATG(2/0)",
"name": "Bse3DI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Bondar, T.S., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsrDI",
"organism": "Bacillus stearothermophilus 3D",
"methylation_site": ""
},
"cfr37i": {
"target_site": "CCGCGG",
"name": "Cfr37I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Lebionka, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Citrobacter freundii RFL37",
"methylation_site": ""
},
"psc28i": {
"target_site": "TTCGAA",
"name": "Psc28I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "AsuII",
"organism": "Planktothrix species 28",
"methylation_site": ""
},
"bspaaii": {
"target_site": "T^CTAGA",
"name": "BspAAII",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Alzhanov, D.T., Alzhanova, D.V., Zheleznaya, L.A., Matvienko, N.I., (1998) Biokhimiia, vol. 63, pp. 636-645."
],
"prototype": "XbaI",
"organism": "Bacillus species AA",
"methylation_site": ""
},
"pfl23i": {
"target_site": "GTGCAC",
"name": "Pfl23I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ApaLI",
"organism": "Pseudomonas fluorescens RFL23",
"methylation_site": ""
},
"bssai": {
"target_site": "R^CCGGY",
"name": "BssAI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "V. Bouriotis",
"references": [
"Rina, M., Stratidakis, I., Bouriotis, V., (1990) Nucleic Acids Res., vol. 18, pp. 6161."
],
"prototype": "Cfr10I",
"organism": "Bacillus species",
"methylation_site": ""
},
"bsp5i": {
"target_site": "CCGG",
"name": "Bsp5I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Bacillus species RFL5",
"methylation_site": ""
},
"cauii": {
"target_site": "CC^SGG",
"name": "CauII",
"suppliers": [],
"source": "A. Bingham",
"references": [
"Bennett, S.P., (1987) Ph.D. Thesis, University of Bristol, UK, pp. 1-196.",
"Bingham, A.H.A., Darbyshire, J., (1982) Gene, vol. 18, pp. 87-91.",
"Levi, C., Bickle, T., Unpublished observations.",
"Molemans, F., van Emmelo, J., Fiers, W., (1982) Gene, vol. 18, pp. 93-96."
],
"prototype": "",
"organism": "Chloroflexus aurantiacus",
"methylation_site": ""
},
"r2.ssudat1i": {
"target_site": "GATC",
"name": "R2.SsuDAT1I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Otani, Y., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 500-511."
],
"prototype": "MboI",
"organism": "Streptococcus suis DAT1",
"methylation_site": ""
},
"bth1997i": {
"target_site": "GATC",
"name": "Bth1997I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. novosibirsk",
"methylation_site": ""
},
"bsph43i": {
"target_site": "CCWGG",
"name": "BspH43I",
"suppliers": [],
"source": "ITI 43",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus species",
"methylation_site": ""
},
"bsivi": {
"target_site": "CCWGG",
"name": "BsiVI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus species",
"methylation_site": ""
},
"msp130i": {
"target_site": "?",
"name": "Msp130I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Microcystis species 130",
"methylation_site": ""
},
"pvui": {
"target_site": "CGAT^CG",
"name": "PvuI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 13315",
"references": [
"Gingeras, T.R., Greenough, L., Schildkraut, I., Roberts, R.J., (1981) Nucleic Acids Res., vol. 9, pp. 4525-4536.",
"Katsuragi, N.T., Kawakami, B., Maekawa, Y., European Patent Office, 1994.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Smith, M.D., Longo, M., Gerard, G.F., Chatterjee, D.K., (1992) Nucleic Acids Res., vol. 20, pp. 5743-5747."
],
"prototype": "",
"organism": "Proteus vulgaris",
"methylation_site": ""
},
"sbri": {
"target_site": "?",
"name": "SbrI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Matsui, M., Mise, K., Yoshida, Y., Ishidate, M., (1986) Eisei Shikenjo Hokoku, vol. 104, pp. 92-96."
],
"prototype": "",
"organism": "Salmonella bredeney",
"methylation_site": ""
},
"acri": {
"target_site": "CYCGRG",
"name": "AcrI",
"suppliers": [],
"source": "ATCC 27895",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Anabaenopsis circularis",
"methylation_site": ""
},
"rlui": {
"target_site": "GCCGGC",
"name": "RluI",
"suppliers": [],
"source": "W. Heumann",
"references": [
"Sievert, U., Rosch, A., Unpublished observations.",
"Winkler, K.-P., (1980) Ph.D. Thesis, Erlangen University, W. Germany, pp. 1-135."
],
"prototype": "NaeI",
"organism": "Rhizobium lupini 1",
"methylation_site": ""
},
"bfi89i": {
"target_site": "Y^GGCCR",
"name": "Bfi89I",
"suppliers": [],
"source": "R.M. Teather",
"references": [
"Mohn, W.W., Teather, R.M., (1995) Gene, vol. 155, pp. 131-132."
],
"prototype": "CfrI",
"organism": "Butyrivibrio fibrisolvens OB189",
"methylation_site": ""
},
"chai": {
"target_site": "GATC^",
"name": "ChaI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Afram, C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Corynebacterium halofytica",
"methylation_site": ""
},
"aspbhi": {
"target_site": "YSCNS(8/12)",
"name": "AspBHI",
"suppliers": [],
"source": "A. Krause",
"references": [
"Fomenkov, A., Xu, D., Zheng, Y., Xu, S.-Y., Unpublished observations.",
"Krause, A. et al., (2006) Nat. Biotechnol., vol. 24, pp. 1385-1391.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "",
"organism": "Azoarcus sp. BH72",
"methylation_site": ""
},
"bsp43i": {
"target_site": "CTGCAG",
"name": "Bsp43I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species RFL43",
"methylation_site": ""
},
"bsc4i": {
"target_site": "CCNNNNN^NNGG",
"name": "Bsc4I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "V.E. Repin",
"references": [
"Dedkov, V.S., (2009) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 3-8.",
"Degtyarev, S.K., Unpublished observations.",
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "BsiYI",
"organism": "Bacillus schlegelii 4",
"methylation_site": "2(4)"
},
"bscvi": {
"target_site": "ATCGAT",
"name": "BscVI",
"suppliers": [],
"source": "P. Babu",
"references": [
"Shroff, D., Damodaran, B., Bulla, S., Ramesh, S., Suma, M.A., Kumar, M., Hegde, V., Babu, P., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species",
"methylation_site": ""
},
"hpyf36iii": {
"target_site": "TGCA",
"name": "HpyF36III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori RFL36",
"methylation_site": ""
},
"uba1215i": {
"target_site": "CTGCAG",
"name": "Uba1215I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1215",
"methylation_site": ""
},
"nmedi": {
"target_site": "(12/7)RCCGGY(7/12)",
"name": "NmeDI",
"suppliers": [],
"source": "U. Vogel",
"references": [
"Claus, H., Friedrich, A., Frosch, M., Vogel, U., (2000) J. Bacteriol., vol. 182, pp. 1296-1303.",
"Kwiatek, A., Kobes, M., Olejnik, K., Piekarowicz, A., (2004) Microbiology, vol. 150, pp. 1713-1722.",
"Kwiatek, A., Piekarowicz, A., (2007) Nucleic Acids Res., vol. 35, pp. 6539-6546."
],
"prototype": "Cfr10I",
"organism": "Neisseria meningitidis 2120",
"methylation_site": "2(5),-2(5)"
},
"ecoo34i": {
"target_site": "?",
"name": "EcoO34I",
"suppliers": [],
"source": "K. Mise",
"references": [
"Yoshida, Y., Mise, K., (1986) J. Bacteriol., vol. 165, pp. 357-362."
],
"prototype": "",
"organism": "Escherichia coli H304",
"methylation_site": ""
},
"bsefi": {
"target_site": "?",
"name": "BseFI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus F245",
"methylation_site": ""
},
"ecor11i": {
"target_site": "?",
"name": "EcoR11I",
"suppliers": [],
"source": "ATCC 35330",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR11",
"methylation_site": "?(6)"
},
"bth213i": {
"target_site": "GATC",
"name": "Bth213I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis strain 213",
"methylation_site": ""
},
"uba11i": {
"target_site": "CCWGG",
"name": "Uba11I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL11",
"methylation_site": ""
},
"esp11i": {
"target_site": "GGYRCC",
"name": "Esp11I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL11",
"methylation_site": ""
},
"bpmi": {
"target_site": "CTGGAG(16/14)",
"name": "BpmI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 711",
"references": [
"Degtyarev, S.K., Morgan, R., Unpublished observations.",
"Zhou, J., Zhu, Z., Xu, S.Y., US Patent Office, 2002."
],
"prototype": "GsuI",
"organism": "Bacillus pumilus",
"methylation_site": "?(6)"
},
"ccop95i": {
"target_site": "GCGC",
"name": "CcoP95I",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vitor, J.M.B., Polisson, C., Cabrita, J., Silva, R.V.C., (1993) Acta Gastroenterol. Belg., vol. 56, pp. 41."
],
"prototype": "HhaI",
"organism": "Campylobacter coli P95",
"methylation_site": ""
},
"aniri": {
"target_site": "?",
"name": "AniRI",
"suppliers": [],
"source": "L. Sherman",
"references": [
"Gallagher, M.L., Burke, W.F., (1985) FEMS Microbiol. Lett., vol. 26, pp. 317-321."
],
"prototype": "",
"organism": "Anacystis nidulans R2",
"methylation_site": ""
},
"bspm39i": {
"target_site": "CAG^CTG",
"name": "BspM39I",
"suppliers": [],
"source": "V. Bouriotis",
"references": [
"Kalugin, A.A., Rina, M., Eldarov, M.A., Markaki, M., Korolev, S.V., Samko, O.T., Khoroshutina, E.B., Sikolov, N.N., Bouriotis, V., (1996) Bioorg. Khim., vol. 22, pp. 108-110."
],
"prototype": "PvuII",
"organism": "Bacillus species M39",
"methylation_site": ""
},
"eco233i": {
"target_site": "GGTCTC",
"name": "Eco233I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL233",
"methylation_site": ""
},
"bsii": {
"target_site": "CACGAG(-5/-1)",
"name": "BsiI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.Kh., Kolykhalov, A.A., Rechkunova, N.I., Dedkov, V.S., Zhilkin, P.A., (1990) Mol. Biol. (Mosk), vol. 24, pp. 244-247."
],
"prototype": "",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"pspgi": {
"target_site": "^CCWGG",
"name": "PspGI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 906",
"references": [
"Morgan, R., Xiao, J.-P., Xu, S.-Y., (1998) Appl. Environ. Microbiol., vol. 64, pp. 3669-3673."
],
"prototype": "EcoRII",
"organism": "Pyrococcus species G1H",
"methylation_site": "?(4)"
},
"mzii": {
"target_site": "CAGCTG",
"name": "MziI",
"suppliers": [],
"source": "H. Lechevalier",
"references": [
"Meyertons, J.L., Tilley, B.C., Lechevalier, M.P., Lechevalier, H.A., (1987) J. Ind. Microbiol., vol. 2, pp. 293-303."
],
"prototype": "PvuII",
"organism": "Micromonospora zionensis",
"methylation_site": ""
},
"bst19ii": {
"target_site": "^GATC",
"name": "Bst19II",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Tomilova, J.E., Dedkov, V.S., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus 19",
"methylation_site": ""
},
"cdicd6ii": {
"target_site": "GATC",
"name": "CdiCD6II",
"suppliers": [],
"source": "N. Minton",
"references": [
"Minton, N.P., Purdy, D.A., Elmore, M.J., O'Keeffe, K.M.T., International Patent Office, 2001.",
"Purdy, D., O'Keeffe, T.A., Elmore, M., Herbert, M., McLeod, A., Bokori-Brown, M., Ostrowski, A., Minton, N.P., (2002) Mol. Microbiol., vol. 46, pp. 439-452."
],
"prototype": "MboI",
"organism": "Clostridium difficile CD6",
"methylation_site": "2(6)"
},
"bfri": {
"target_site": "C^TTAAG",
"name": "BfrI",
"suppliers": [
"Roche Applied Science (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "U. Mayr",
"references": [
"Prinz, B., Hardt, A., Schmitz, G., Jarsch, M., Kessler, C., Unpublished observations."
],
"prototype": "AflII",
"organism": "Bacteroides fragilis",
"methylation_site": ""
},
"scui": {
"target_site": "CTCGAG",
"name": "ScuI",
"suppliers": [],
"source": "KCC S0316",
"references": [
"Shimotsu, H., Takahashi, H., Saito, H., (1980) Agric. Biol. Chem., vol. 44, pp. 1665-1666."
],
"prototype": "XhoI",
"organism": "Streptomyces cupidosporus",
"methylation_site": ""
},
"unbi": {
"target_site": "^GGNCC",
"name": "UnbI",
"suppliers": [],
"source": "P.P. Stepien",
"references": [
"Kawalec, M., Borsuk, P., Piechula, S., Stepien, P.P., (1997) Acta Biochim. Pol., vol. 44, pp. 849-852."
],
"prototype": "AsuI",
"organism": "Unidentified bacterium strain #8",
"methylation_site": ""
},
"kpnk14i": {
"target_site": "GGTACC",
"name": "KpnK14I",
"suppliers": [],
"source": "W. Piepersberg",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Klebsiella pneumoniae mmK14",
"methylation_site": ""
},
"bsp93i": {
"target_site": "CTGCAG",
"name": "Bsp93I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species RFL93",
"methylation_site": ""
},
"bci29i": {
"target_site": "AT^CGAT",
"name": "Bci29I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "ClaI",
"organism": "Bacillus circulans 29",
"methylation_site": ""
},
"haeii": {
"target_site": "RGCGC^Y",
"name": "HaeII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)"
],
"source": "ATCC 11116",
"references": [
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Roberts, R.J., Breitmeyer, J.B., Tabachnik, N.F., Myers, P.A., (1975) J. Mol. Biol., vol. 91, pp. 121-123.",
"Stein, D.C., Gunn, J.S., Piekarowicz, A., (1998) Biol. Chem., vol. 379, pp. 575-578.",
"Tu, C.-P.D., Roychoudhury, R., Wu, R., (1976) Biochem. Biophys. Res. Commun., vol. 72, pp. 355-362."
],
"prototype": "",
"organism": "Haemophilus aegyptius",
"methylation_site": "?(5)"
},
"sprli": {
"target_site": "CTGCAG",
"name": "SprLI",
"suppliers": [],
"source": "ATCC 15825",
"references": [
"Saboohi, N., Sohail, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Streptomyces prasinus",
"methylation_site": ""
},
"m.etatxddam": {
"target_site": "GATC",
"name": "M.EtaTXDDam",
"suppliers": [],
"source": "K. Sun",
"references": [
"Sun, K., Jiao, X.D., Zhang, M., Sun, L., (2010) Vet. Microbiol., vol. 141, pp. 149-154."
],
"prototype": "MboI",
"organism": "Edwardsiella tarda",
"methylation_site": "2(6)"
},
"rleai": {
"target_site": "CCCACA(12/9)",
"name": "RleAI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Jarsch, M., Schmitz-Agheguian, G., Kessler, C., European Patent Office, 1989.",
"Vesely, Z., Muller, A., Schmitz, G.G., Kaluza, K., Jarsch, M., Kessler, C., (1990) Gene, vol. 95, pp. 129-131."
],
"prototype": "",
"organism": "Rhizobium leguminosarum",
"methylation_site": ""
},
"asplei": {
"target_site": "GCG^C",
"name": "AspLEI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Arthrobacter species LE3860",
"methylation_site": ""
},
"fspei": {
"target_site": "CC(12/16)",
"name": "FspEI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "L.S. Tisa",
"references": [
"Copeland, A. et al., Unpublished observations.",
"Zheng, Y., Roberts, R.J., International Patent Office, 2010."
],
"prototype": "",
"organism": "Frankia sp. EAN1pec",
"methylation_site": ""
},
"vcho52i": {
"target_site": "?",
"name": "VchO52I",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "",
"organism": "Vibrio cholerae 207-73",
"methylation_site": ""
},
"uba1335i": {
"target_site": "CTCGAG",
"name": "Uba1335I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1335",
"methylation_site": ""
},
"haeiv": {
"target_site": "(7/13)GAYNNNNNRTC(14/9)",
"name": "HaeIV",
"suppliers": [],
"source": "ATCC 11116",
"references": [
"Piekarowicz, A., Golaszewska, M., Sunday, A.O., Siwinska, M., Stein, D.C., (1999) J. Mol. Biol., vol. 293, pp. 1055-1065."
],
"prototype": "",
"organism": "Haemophilus aegyptius",
"methylation_site": "2(6)"
},
"bse24i": {
"target_site": "CC^WGG",
"name": "Bse24I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus species 24",
"methylation_site": ""
},
"aspa2i": {
"target_site": "C^CTAGG",
"name": "AspA2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "AvrII",
"organism": "Arthrobacter species A2",
"methylation_site": ""
},
"hpyf48iii": {
"target_site": "TGCA",
"name": "HpyF48III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori RFL48",
"methylation_site": ""
},
"bspj76i": {
"target_site": "CGCG",
"name": "BspJ76I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Bacillus species RFL76",
"methylation_site": ""
},
"pae25i": {
"target_site": "CTGCAG",
"name": "Pae25I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL25",
"methylation_site": ""
},
"uba1293i": {
"target_site": "GGCC",
"name": "Uba1293I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1293",
"methylation_site": ""
},
"uba1377i": {
"target_site": "GGCC",
"name": "Uba1377I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1377",
"methylation_site": ""
},
"hpyf59iii": {
"target_site": "TCGA",
"name": "HpyF59III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL59",
"methylation_site": ""
},
"eciei": {
"target_site": "GGGCCC",
"name": "EciEI",
"suppliers": [],
"source": "B. Levin",
"references": [
"Croft, R., Levin, B., Unpublished observations."
],
"prototype": "ApaI",
"organism": "Escherichia coli T39",
"methylation_site": ""
},
"bpuei": {
"target_site": "CTTGAG(16/14)",
"name": "BpuEI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1378",
"references": [
"Murray, I.A., Wei, H., Unpublished observations.",
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "Bce83I",
"organism": "Bacillus pumilus 2187a",
"methylation_site": "?(6)"
},
"dpai": {
"target_site": "AGT^ACT",
"name": "DpaI",
"suppliers": [],
"source": "IAM 14115",
"references": [
"Suzuki, T., Sato, Y., Yamada, Y., Akagawa-Matsushita, M., Yamasato, K., (1991) Agric. Biol. Chem., vol. 55, pp. 2647-2649."
],
"prototype": "ScaI",
"organism": "Deleya pacifica",
"methylation_site": ""
},
"cfr11i": {
"target_site": "CCWGG",
"name": "Cfr11I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL11",
"methylation_site": ""
},
"bspri": {
"target_site": "GG^CC",
"name": "BspRI",
"suppliers": [],
"source": "P. Venetianer",
"references": [
"Kim, S.C., Posfai, G., Szybalski, W., (1991) Gene, vol. 100, pp. 45-50.",
"Kiss, A., Sain, B., Csordas-Toth, E., Venetianer, P., (1977) Gene, vol. 1, pp. 323-329.",
"Koncz, C., Kiss, A., Venetianer, P., (1978) Eur. J. Biochem., vol. 89, pp. 523-529.",
"Posfai, G., Kiss, A., Erdei, S., Posfai, J., Venetianer, P., (1983) J. Mol. Biol., vol. 170, pp. 597-610.",
"Posfai, G., Szybalski, W., (1988) Gene, vol. 74, pp. 179-181.",
"Szilak, L., Finta, C., Patthy, A., Venetianer, P., Kiss, A., (1994) Nucleic Acids Res., vol. 22, pp. 2876-2881."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus R",
"methylation_site": "3(5)"
},
"sau33i": {
"target_site": "GGNCC",
"name": "Sau33I",
"suppliers": [],
"source": "I. Mikula",
"references": [
"Godany, A., Bukovska, G., Farkasovska, J., Brnakova, Z., Dmitriev, A., Tkacikova, L., Ayele, T., Mikula, I., (2004) Folia Microbiol. (Praha), vol. 49, pp. 307-314."
],
"prototype": "AsuI",
"organism": "Staphylococcus aureus 33",
"methylation_site": ""
},
"trskti": {
"target_site": "GATC",
"name": "TrsKTI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193.",
"Piknova, M., Pristas, P., Javorsky, P., Kasperowic, A., Michalowski, T., (2004) Lett. Appl. Microbiol., vol. 38, pp. 311-314."
],
"prototype": "MboI",
"organism": "Treponema species kT",
"methylation_site": "2(6)"
},
"saus2i": {
"target_site": "?",
"name": "SauS2I",
"suppliers": [],
"source": "R. Novick",
"references": [
"Sjostrom, J.E., Lofdahl, S., Philipson, L., (1978) J. Bacteriol., vol. 133, pp. 1144-1149."
],
"prototype": "",
"organism": "Staphylococcus aureus RN450",
"methylation_site": ""
},
"patai": {
"target_site": "GGCGCC",
"name": "PatAI",
"suppliers": [],
"source": "ATCC 19262",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "NarI",
"organism": "Pseudomonas atlantica",
"methylation_site": ""
},
"tsp8ei": {
"target_site": "GCCNNNN^NGGC",
"name": "Tsp8EI",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Williams, R.A.D., (1995) Biochem. J., vol. 309, pp. 595-599."
],
"prototype": "BglI",
"organism": "Thermus species 8E",
"methylation_site": ""
},
"asp14hi": {
"target_site": "RGATCY",
"name": "Asp14HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Acidiphilium species 14H",
"methylation_site": ""
},
"sauei": {
"target_site": "GATC",
"name": "SauEI",
"suppliers": [],
"source": "NCTC 9315",
"references": [
"Stobberingh, E.E., Schiphof, R., Sussenbach, J.S., (1977) J. Bacteriol., vol. 131, pp. 645-649."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus 71",
"methylation_site": ""
},
"asp36hi": {
"target_site": "GAATGC(1/-1)",
"name": "Asp36HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Kobayashi, F., Dou, D., Nomura, Y., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Acidiphilium species 36H",
"methylation_site": ""
},
"bpu1102i": {
"target_site": "GC^TNAGC",
"name": "Bpu1102I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Trinkunaite, L., Maneliene, Z., Kiuduliene, L., Petrusyte, M., Butkus, V., Unpublished observations."
],
"prototype": "EspI",
"organism": "Bacillus pumilus RFL1102",
"methylation_site": ""
},
"nspsaiii": {
"target_site": "CCATGG",
"name": "NspSAIII",
"suppliers": [],
"source": "D. Jones",
"references": [
"Dean, P.D.C., Walker, J.N.B., (1985) Biochem. Soc. Trans., vol. 13, pp. 1055-1058."
],
"prototype": "NcoI",
"organism": "Nostoc species SA",
"methylation_site": ""
},
"rsplki": {
"target_site": "GCATG^C",
"name": "RspLKI",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Zabaznaya, E.V., Zheleznaya, L.A., Matvienko, N.I., (1997) Biokhimiia, vol. 62, pp. 1018-1028."
],
"prototype": "SphI",
"organism": "Rhodococcus species LK2",
"methylation_site": ""
},
"gsei": {
"target_site": "GGNCC",
"name": "GseI",
"suppliers": [],
"source": "ATCC 29159",
"references": [
"Brand, P., de Waard, A., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Gloeocapsa species",
"methylation_site": ""
},
"bsp138i": {
"target_site": "GATC",
"name": "Bsp138I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL138",
"methylation_site": ""
},
"hpy99i": {
"target_site": "CGWCG^",
"name": "Hpy99I",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "R.A. Alm",
"references": [
"Alm, R.A., Ling, L.-S.L., Moir, D.T., King, B.L., Brown, E.D., Doig, P.C., Smith, D.R., Noonan, B., Guild, B.C., deJonge, B.L., Carmel, G., Tummino, P.J., Caruso, A., Uria-Nickelsen, M., Mills, D.M., Ives, C., Gibson, R., Merberg, D., Mills, S., (1999) Nature, vol. 397, pp. 176-180.",
"Lin, L., Porter, N., Kong, H., Unpublished observations.",
"Morgan, R.D., Xu, Q., International Patent Office, 2001.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Helicobacter pylori J99",
"methylation_site": "?(4)"
},
"ecoo65i": {
"target_site": "G^GTNACC",
"name": "EcoO65I",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "K. Mise",
"references": [
"Tsui, W.-C., Unpublished observations."
],
"prototype": "BstEII",
"organism": "Escherichia coli K11a",
"methylation_site": ""
},
"mlu9273ii": {
"target_site": "GCCGGC",
"name": "Mlu9273II",
"suppliers": [],
"source": "ATCC 9273",
"references": [
"Nelson, M., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Micrococcus luteus",
"methylation_site": ""
},
"cfrbi": {
"target_site": "C^CWWGG",
"name": "CfrBI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Beletskaya, I.V., Zakharova, M.V., Shlyapnikov, M.G., Semenova, L.M., Solonin, A.S., (2000) Nucleic Acids Res., vol. 28, pp. 3817-3822.",
"Zakharova, M.V., Kravetz, A.N., Beletzkaja, I.V., Repyk, A.V., Solonin, A.S., (1993) Gene, vol. 129, pp. 77-81."
],
"prototype": "StyI",
"organism": "Citrobacter freundii 4111",
"methylation_site": "1(4)"
},
"bstz14i": {
"target_site": "?",
"name": "BstZ14I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus 36N",
"methylation_site": ""
},
"ccop95ii": {
"target_site": "GATC",
"name": "CcoP95II",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vitor, J.M.B., Polisson, C., Cabrita, J., Silva, R.V.C., (1993) Acta Gastroenterol. Belg., vol. 56, pp. 41."
],
"prototype": "MboI",
"organism": "Campylobacter coli P95",
"methylation_site": ""
},
"esphk22i": {
"target_site": "CCWGG",
"name": "EspHK22I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Enterobacter species N22",
"methylation_site": ""
},
"rsprsi": {
"target_site": "GCCNNNNNGGC",
"name": "RspRSI",
"suppliers": [],
"source": "D. Bryant",
"references": [
"Copeland, A. et al., Unpublished observations.",
"Wang, H., Vaisvila, R., Wilson, G.G., Unpublished observations."
],
"prototype": "BglI",
"organism": "Roseiflexus sp. RS-1",
"methylation_site": ""
},
"bmeri": {
"target_site": "GACNNN^NNGTC",
"name": "BmeRI",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "Vivantis 32",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "Eam1105I",
"organism": "Bacillus megaterium",
"methylation_site": ""
},
"gst1588ii": {
"target_site": "GATC",
"name": "Gst1588II",
"suppliers": [],
"source": "NEB 1588",
"references": [
"Pan, X.S., Morgan, R., Unpublished observations."
],
"prototype": "MboI",
"organism": "Geobacillus stearothermophilus",
"methylation_site": ""
},
"sve194i": {
"target_site": "CTCGAG",
"name": "Sve194I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces verticillius 194",
"methylation_site": ""
},
"bsp1591ii": {
"target_site": "CCGG",
"name": "Bsp1591II",
"suppliers": [],
"source": "NEB 1300",
"references": [
"Nkenfou, C., Polisson, C., Nkenfou, J., Notedji, A., Morgan, R., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Bacillus sphaericus 1591",
"methylation_site": ""
},
"bsidi": {
"target_site": "GGCC",
"name": "BsiDI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus species",
"methylation_site": ""
},
"bce751i": {
"target_site": "G^GATCC",
"name": "Bce751I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kramarov, V.M., Skrypina, N.A., Smolyaninov, V.V., Smirnov, V.V., Resnik, S.R., Sorokulova, I.B., Matvienko, N.I., (1989) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 42-45."
],
"prototype": "BamHI",
"organism": "Bacillus cereus 751",
"methylation_site": ""
},
"m.mbuiii": {
"target_site": "?",
"name": "M.MbuIII",
"suppliers": [],
"source": "S.S. Debov",
"references": [
"Nikolskaya, I.I., Sharkova, E.V., Suchkov, S.V., Karpets, L.Z., Debov, S.S., Somodi, P., Foldes, I., (1983) Biochem. Int., vol. 7, pp. 605-615."
],
"prototype": "",
"organism": "Mycobacterium butyricum",
"methylation_site": ""
},
"bstez359i": {
"target_site": "GTT^AAC",
"name": "BstEZ359I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "HpaI",
"organism": "Bacillus stearothermophilus EZ359",
"methylation_site": ""
},
"draii": {
"target_site": "RG^GNCCY",
"name": "DraII",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "ATCC 27603",
"references": [
"Benner, J.S., Unpublished observations.",
"de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., (1985) FEBS Lett., vol. 180, pp. 219-223.",
"Grosskopf, R., Wolf, W., Kessler, C., (1985) Nucleic Acids Res., vol. 13, pp. 1517-1528."
],
"prototype": "",
"organism": "Deinococcus radiophilus",
"methylation_site": ""
},
"mbvi": {
"target_site": "?",
"name": "MbvI",
"suppliers": [],
"source": "ATCC 17947",
"references": [
"Jiang, B.D., Myers, P., Unpublished observations."
],
"prototype": "",
"organism": "Moraxella bovis",
"methylation_site": ""
},
"asp40hi": {
"target_site": "GAATGC(1/-1)",
"name": "Asp40HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Kobayashi, F., Dou, D., Nomura, Y., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Acidiphilium species 40H",
"methylation_site": ""
},
"sinmii": {
"target_site": "?",
"name": "SinMII",
"suppliers": [],
"source": "ATCC 29663",
"references": [
"Camp, R., Schildkraut, I., Unpublished observations."
],
"prototype": "",
"organism": "Staphylococcus intermedius",
"methylation_site": ""
},
"ndai": {
"target_site": "GG^CGCC",
"name": "NdaI",
"suppliers": [],
"source": "ATCC 21944",
"references": [
"Comb, D.G., Hess, E.J., Wilson, G., Unpublished observations."
],
"prototype": "NarI",
"organism": "Nocardia dassonvillei",
"methylation_site": ""
},
"cspci": {
"target_site": "(11/13)CAANNNNNGTGG(12/10)",
"name": "CspCI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1398",
"references": [
"Lunnen, K.D., Heiter, D.F., Benner, J.S., Wilson, G.G., Unpublished observations.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R., Nkenfou, C.N., US Patent Office, 2005.",
"Morgan, R., Wilson, G., Lunnen, K., Heiter, D., Benner, J., Nkenfou, C.N., Picone, S., International Patent Office, 2005."
],
"prototype": "",
"organism": "Citrobacter species 2144",
"methylation_site": "?(6)"
},
"vnei": {
"target_site": "G^TGCAC",
"name": "VneI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Rechkunova, N.I., Netesova, N.A., Tchigikov, V.E., Malygin, E.G., Kochkin, A.V., Mikhajlov, V.V., Rasskazov, V.A., (1987) Bioorg. Khim., vol. 13, pp. 422-423."
],
"prototype": "ApaLI",
"organism": "Vibrio nereis 18",
"methylation_site": ""
},
"uba1317i": {
"target_site": "GATC",
"name": "Uba1317I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1317",
"methylation_site": ""
},
"uban1i": {
"target_site": "GATC",
"name": "UbaN1I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "MboI",
"organism": "Unidentified bacterium OQ3-07, Nicaragua",
"methylation_site": ""
},
"m.spri": {
"target_site": "GGCC,CCGG",
"name": "M.SPRI",
"suppliers": [],
"source": "T.A. Trautner",
"references": [
"Behrens, B., Pawlek, B., Morelli, G., Trautner, T.A., (1983) Mol. Gen. Genet., vol. 189, pp. 10-16.",
"Gunthert, U., Reiners, L., (1987) Nucleic Acids Res., vol. 15, pp. 3689-3701.",
"Jentsch, S., Gunthert, U., Trautner, T.A., (1981) Nucleic Acids Res., vol. 9, pp. 2753-2759.",
"Posfai, G., Baldauf, F., Erdei, S., Posfai, J., Venetianer, P., Kiss, A., (1984) Nucleic Acids Res., vol. 12, pp. 9039-9049.",
"Trautner, T.A., Pawlek, B., Gunthert, U., Canosi, U., Jentsch, S., Freund, M., (1980) Mol. Gen. Genet., vol. 180, pp. 361-367."
],
"prototype": "HaeIII",
"organism": "Mutation screening vector pSPRX",
"methylation_site": "3(5), 1(5), 2(5)"
},
"bspk1ai": {
"target_site": "?",
"name": "BspK1aI",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "",
"organism": "Bacillus sphaericus K",
"methylation_site": ""
},
"bsp101i": {
"target_site": "TTCGAA",
"name": "Bsp101I",
"suppliers": [],
"source": "ITI 101",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"bba179i": {
"target_site": "WCCGGW",
"name": "Bba179I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Repin, V.E., Rechkunova, N.I., Shevchenko, A.V., Abdurashitov, M.A., Russian Patent Office, 1996."
],
"prototype": "BetI",
"organism": "Bacillus badius BKPM B-6616",
"methylation_site": ""
},
"bst16i": {
"target_site": "RGCGCY",
"name": "Bst16I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus stearothermophilus M16",
"methylation_site": ""
},
"ngopiii": {
"target_site": "CCGC^GG",
"name": "NgoPIII",
"suppliers": [],
"source": "J.R. Saunders",
"references": [
"Sullivan, K.M., Macdonald, H.J., Saunders, J.R., (1987) FEMS Microbiol. Lett., vol. 44, pp. 389-393.",
"Sullivan, K.M., Saunders, J.R., (1988) Gonococci and Meningococci, ed. Poolman, J.T. (Kluwer Academic Publishers, Dordrecht, The Netherlands), vol. 0, pp. 329-334."
],
"prototype": "SacII",
"organism": "Neisseria gonorrhoeae P9-2",
"methylation_site": ""
},
"uba1382i": {
"target_site": "GAATGC",
"name": "Uba1382I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Unidentified bacterium RFL1382",
"methylation_site": ""
},
"ppei": {
"target_site": "GGGCC^C",
"name": "PpeI",
"suppliers": [],
"source": "CCAP 1462/5",
"references": [
"Piechula, S., Piosik, J., Bielawski, K., Podhajska, A.J., (1996) Mol. Biotechnol., vol. 5, pp. 97-99."
],
"prototype": "ApaI",
"organism": "Phormidium persicinum",
"methylation_site": ""
},
"mlyi": {
"target_site": "GAGTC(5/5)",
"name": "MlyI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NBL 2048",
"references": [
"Eastlake, P., Unpublished observations.",
"Kong, H., Unpublished observations.",
"Kong, H., Higgins, L.S., US Patent Office, 2002.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "PleI",
"organism": "Micrococcus lylae",
"methylation_site": "2(6)"
},
"atuii": {
"target_site": "CCWGG",
"name": "AtuII",
"suppliers": [],
"source": "C.I. Kado",
"references": [
"LeBon, J.M., Kado, C.I., Rosenthal, L.J., Chirikjian, J.G., (1978) Proc. Natl. Acad. Sci. U. S. A., vol. 75, pp. 4097-4101."
],
"prototype": "EcoRII",
"organism": "Agrobacterium tumefaciens ID 135",
"methylation_site": ""
},
"hpyf55ii": {
"target_site": "GANTC",
"name": "HpyF55II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL55",
"methylation_site": ""
},
"acc38i": {
"target_site": "CCWGG",
"name": "Acc38I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Acinetobacter calcoaceticus 38",
"methylation_site": ""
},
"srl55dii": {
"target_site": "ATTAAT",
"name": "Srl55DII",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "VspI",
"organism": "Selenomonas ruminantium subsp. lactilytica 55",
"methylation_site": ""
},
"uba1438i": {
"target_site": "GGWCC",
"name": "Uba1438I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1438",
"methylation_site": ""
},
"bstsci": {
"target_site": "^CCNGG",
"name": "BstSCI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Bacillus stearothermophilus SC",
"methylation_site": ""
},
"bsp65i": {
"target_site": "GATC",
"name": "Bsp65I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL65",
"methylation_site": ""
},
"hgai": {
"target_site": "GACGC(5/10)",
"name": "HgaI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "ATCC 14385",
"references": [
"Brown, N.L., Smith, M., (1977) Proc. Natl. Acad. Sci. U. S. A., vol. 74, pp. 3213-3216.",
"Landry, D., Barsomian, J.M., Feehery, G.R., Wilson, G.G., (1992) Methods Enzymol., vol. 216, pp. 244-259.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Moses, P., Horiuchi, K., (1979) J. Mol. Biol., vol. 135, pp. 517-524.",
"Sugisaki, H., (1978) Gene, vol. 3, pp. 17-28.",
"Sugisaki, H., Yamamoto, K., Takanami, M., (1991) J. Biol. Chem., vol. 266, pp. 13952-13957.",
"Takanami, M., (1974) Methods Mol. Biol., vol. 7, pp. 113-133.",
"Wilson, G.G., Murray, N.E., (1991) Annu. Rev. Genet., vol. 25, pp. 585-627.",
"Zinovev, V.V., Rechkunova, N.I., Gorbunov, Y.A., Buryanov, Y.I., Malygin, E.G., (1989) Biopol. Kletka, vol. 5, pp. 22-27."
],
"prototype": "",
"organism": "Haemophilus gallinarum",
"methylation_site": "?(5),-2(5)"
},
"csp1470i": {
"target_site": "GCGC",
"name": "Csp1470I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Clostridium sporogenes 1470",
"methylation_site": ""
},
"uba1401i": {
"target_site": "CCSGG",
"name": "Uba1401I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1401",
"methylation_site": ""
},
"tsp133i": {
"target_site": "GATC",
"name": "Tsp133I",
"suppliers": [],
"source": "ITI 133",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MboI",
"organism": "Thermus species",
"methylation_site": ""
},
"msp23ii": {
"target_site": "CTCGAG",
"name": "Msp23II",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Andreeva, I.S., Puchkova, L.I., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Micrococcus species 23",
"methylation_site": ""
},
"gcegli": {
"target_site": "CCGC^GG",
"name": "GceGLI",
"suppliers": [],
"source": "IFO 3285",
"references": [
"Sasaki, J., Murakami, M., Yamada, Y., (1985) Agric. Biol. Chem., vol. 49, pp. 3017-3022."
],
"prototype": "SacII",
"organism": "Gluconobacter cerinus",
"methylation_site": ""
},
"hpyf56i": {
"target_site": "ACNGT",
"name": "HpyF56I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL56",
"methylation_site": ""
},
"eco1524i": {
"target_site": "AGG^CCT",
"name": "Eco1524I",
"suppliers": [],
"source": "H. Lazim",
"references": [
"Lazim, H., Josephsen, J., Ben Hassen, A., Belhadj, O., Limam, R., (2005) Appl. Biochem. Biotechnol., vol. 125, pp. 189-199.",
"Lazim, H., Mankai, H., Limam, F., Unpublished observations."
],
"prototype": "StuI",
"organism": "Escherichia coli 1524",
"methylation_site": ""
},
"bsrpi": {
"target_site": "?",
"name": "BsrPI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus P5",
"methylation_site": ""
},
"asp123ii": {
"target_site": "?",
"name": "Asp123II",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Anabaena species 123",
"methylation_site": ""
},
"uba1447i": {
"target_site": "TGATCA",
"name": "Uba1447I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BclI",
"organism": "Unidentified bacterium RFL1447",
"methylation_site": ""
},
"bshni": {
"target_site": "G^GYRCC",
"name": "BshNI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maniusyte, V., Lazareviciute, L., Jurgelyte, R., Maneliene, Z., Velickiene, Z., Norgeliene, D., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Bacillus sphaericus TK-45",
"methylation_site": ""
},
"spmi": {
"target_site": "AT^CGAT",
"name": "SpmI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Radionenko, Y.V., Ushakova, T.A., Repin, V.E., Russian Patent Office, 2005.",
"Ushakova, T.A., Puchkova, L.I., Radionenko, Y.V., Gutorov, V.V., Kuvshinov, V.N., Repin, V.E., (2004) Biotekhnologiya, vol. 0, pp. 38-42."
],
"prototype": "ClaI",
"organism": "Sphingobacterium mizutae",
"methylation_site": ""
},
"mmai": {
"target_site": "CTGCAG",
"name": "MmaI",
"suppliers": [],
"source": "D. Boone",
"references": [
"Tumbula, D.L., Makula, R.A., Whitman, W.B., (1994) FEMS Microbiol. Lett., vol. 121, pp. 309-314."
],
"prototype": "PstI",
"organism": "Methanococcus maripaludis JJ",
"methylation_site": ""
},
"uba1318i": {
"target_site": "CCSGG",
"name": "Uba1318I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1318",
"methylation_site": ""
},
"bsmhi": {
"target_site": "RGCGCY",
"name": "BsmHI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus stearothermophilus H426",
"methylation_site": ""
},
"eco71ki": {
"target_site": "GGTCTC",
"name": "Eco71KI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Denjmukhametov, M.M., Kravets, A.N., Solonin, A.S., Russian Patent Office, 1996."
],
"prototype": "Eco31I",
"organism": "Escherichia coli VKM V-2006D",
"methylation_site": ""
},
"nflii": {
"target_site": "?",
"name": "NflII",
"suppliers": [],
"source": "NRCC 31011",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria flavescens",
"methylation_site": ""
},
"tthrqi": {
"target_site": "TCGA",
"name": "TthRQI",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Al-Awadhi, S., Williams, R.A.D., (1998) Microbiology, vol. 144, pp. 167-175."
],
"prototype": "TaqI",
"organism": "Thermus thermophilus RQ1",
"methylation_site": ""
},
"bloi": {
"target_site": "?",
"name": "BloI",
"suppliers": [],
"source": "ATCC 15707",
"references": [
"Sakurai, T., Kosaka, T., US Patent Office, 1983."
],
"prototype": "",
"organism": "Bifidobacterium longum E194b",
"methylation_site": ""
},
"ssebi": {
"target_site": "AGG^CCT",
"name": "SseBI",
"suppliers": [
"Minotech Biotechnology (1/11)"
],
"source": "V. Bouriotis",
"references": [
"Rina, M., Tzanodaskalaki, M., Karagouni, A., Pagomenou, M., Bouriotis, V., (1992) Nucleic Acids Res., vol. 20, pp. 1808."
],
"prototype": "StuI",
"organism": "Streptomyces species",
"methylation_site": ""
},
"sausi": {
"target_site": "GCC^GGC",
"name": "SauSI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Pristas, P., Godany, A., Sevcikova, B., Oktavcova, B., Farkasovska, J., (1992) Nucleic Acids Res., vol. 20, pp. 4364."
],
"prototype": "NaeI",
"organism": "Streptomyces aureofaciens 16",
"methylation_site": ""
},
"bspj105i": {
"target_site": "GGWCC",
"name": "BspJ105I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus species RFL105",
"methylation_site": ""
},
"sflhk10695i": {
"target_site": "CCSGG",
"name": "SflHK10695I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "CauII",
"organism": "Shigella flexneri 10695",
"methylation_site": ""
},
"m.cvisii": {
"target_site": "CATG",
"name": "M.CviSII",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Que, Q., Zhang, Y., Nelson, M., Ropp, S., Burbank, D.E., Van Etten, J.L., (1997) Gene, vol. 190, pp. 237-244."
],
"prototype": "NlaIII",
"organism": "Chlorella strain NC64A (SC-1A)",
"methylation_site": "2(6)"
},
"ssu220i": {
"target_site": "GATC",
"name": "Ssu220I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis strain 220",
"methylation_site": ""
},
"btu34i": {
"target_site": "GATC",
"name": "Btu34I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Burtseva, L.I., Burlak, V.A., Trusova, S.I., (1991) Mol. Gen. Mikrobiol. Virusol., vol. 11, pp. 20-22."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis 34",
"methylation_site": ""
},
"hpyf16i": {
"target_site": "TCGA",
"name": "HpyF16I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL16",
"methylation_site": ""
},
"bst158i": {
"target_site": "CTCTTC",
"name": "Bst158I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "Ksp632I",
"organism": "Bacillus stearothermophilus P158",
"methylation_site": ""
},
"bpuami": {
"target_site": "GAG^CTC",
"name": "BpuAmI",
"suppliers": [],
"source": "J.M. Chies",
"references": [
"Chies, J.M., de O-Dias, A.C., Maia, H.M.M., Astolfi-Filho, S., Unpublished observations."
],
"prototype": "SacI",
"organism": "Bacillus pumilus",
"methylation_site": ""
},
"uba1391i": {
"target_site": "CCNGG",
"name": "Uba1391I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Unidentified bacterium RFL1391",
"methylation_site": ""
},
"chyi": {
"target_site": "AGGCCT",
"name": "ChyI",
"suppliers": [],
"source": "ATCC 21628",
"references": [
"Hall, D., Unpublished observations."
],
"prototype": "StuI",
"organism": "Corynebacterium hydrocarboclastum",
"methylation_site": ""
},
"sfni": {
"target_site": "GGWCC",
"name": "SfnI",
"suppliers": [],
"source": "ATCC 29938",
"references": [
"Stote, R., Schildkraut, I., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Serratia fonticola",
"methylation_site": ""
},
"kaz48ki": {
"target_site": "RGGNC^CY",
"name": "Kaz48kI",
"suppliers": [],
"source": "A.N. Kravets",
"references": [
"Pertsev, A.V., Kravets, A.N., Russian Patent Office, 1995."
],
"prototype": "DraII",
"organism": "Klebsiella azeanae",
"methylation_site": ""
},
"eco193i": {
"target_site": "CCWGG",
"name": "Eco193I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL193",
"methylation_site": ""
},
"m.smaii": {
"target_site": "GATC",
"name": "M.SmaII",
"suppliers": [],
"source": "ATCC 49779",
"references": [
"Ostendorf, T., Cherepanov, P., deVries, J., Wachernagel, W., (1999) J. Bacteriol., vol. 181, pp. 3880-3885."
],
"prototype": "MboI",
"organism": "Serratia marcescens Sb",
"methylation_site": "2(6)"
},
"tgli": {
"target_site": "CCGCGG",
"name": "TglI",
"suppliers": [],
"source": "ATCC 15345",
"references": [
"Gingeras, T.R., Roberts, R.J., Unpublished observations."
],
"prototype": "SacII",
"organism": "Thermopolyspora glauca",
"methylation_site": ""
},
"maek81i": {
"target_site": "C^GTACG",
"name": "MaeK81I",
"suppliers": [],
"source": "M. Shirai",
"references": [
"Takahashi, I., Hayano, D., Asayama, M., Masahiro, F., Watahiki, M., Shirai, M., (1996) FEMS Microbiol. Lett., vol. 145, pp. 107-111."
],
"prototype": "SplI",
"organism": "Microcystis aeruginosa K-81",
"methylation_site": ""
},
"tauii": {
"target_site": "CGGCCG",
"name": "TauII",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Maneliene, Z., Janulaitis, A., (2001) FEMS Microbiol. Lett., vol. 204, pp. 253-257."
],
"prototype": "XmaIII",
"organism": "Thermus aquaticus Ma2 3-1",
"methylation_site": ""
},
"rrui": {
"target_site": "TCG^CGA",
"name": "RruI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G359",
"references": [
"Vitkute, J., Lauciuniene, N., Lapcinskaja, S., Trinkunaite, L., Zakareviciene, L., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "NruI",
"organism": "Rhodococcus ruber RFL5",
"methylation_site": ""
},
"clai": {
"target_site": "AT^CGAT",
"name": "ClaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)"
],
"source": "ATCC 49862",
"references": [
"Longo, M.C., Smith, M.D., Chatterjee, D.K., US Patent Office, 1994.",
"Mayer, H., Grosschedl, R., Schutte, H., Hobom, G., (1981) Nucleic Acids Res., vol. 9, pp. 4833-4845.",
"McClelland, M., (1981) Nucleic Acids Res., vol. 9, pp. 6795-6804.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Caryophanon latum L",
"methylation_site": "5(6)"
},
"sdeai": {
"target_site": "CAGRAG(21/19)",
"name": "SdeAI",
"suppliers": [],
"source": "ATCC 33889",
"references": [
"Copeland, A., Lucas, S., Lapidus, A., Barry, K., Detter, J.C., Glavina, T., Hammon, N., Israni, S., Pitluck, S., Chain, P., Malfatti, S., Shin, M., Vergez, L., Schmutz, J., Larimer, F., Land, M., Kyrpides, N., Lykidis, A., Richardson, P., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Sulfurimonas denitrificans",
"methylation_site": "?(6)"
},
"cfoi": {
"target_site": "GCG^C",
"name": "CfoI",
"suppliers": [
"Roche Applied Science (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)"
],
"source": "ATCC 23439",
"references": [
"Makula, R.A., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Clostridium formicoaceticum",
"methylation_site": ""
},
"cfr7i": {
"target_site": "GGTNACC",
"name": "Cfr7I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485."
],
"prototype": "BstEII",
"organism": "Citrobacter freundii RFL7",
"methylation_site": ""
},
"hpybi": {
"target_site": "GT^AC",
"name": "HpyBI",
"suppliers": [],
"source": "N.L. Brown",
"references": [
"Ivic, A., Jakeman, K.J., Penn, C.W., Brown, N.L., (1999) FEMS Microbiol. Lett., vol. 179, pp. 175-180."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori strain Roberts",
"methylation_site": ""
},
"trsktii": {
"target_site": "GACNNNGTC",
"name": "TrsKTII",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Javorsky, P., Pristas, P., (2005) FEMS Microbiol. Lett., vol. 251, pp. 99."
],
"prototype": "Tth111I",
"organism": "Treponema species kT",
"methylation_site": ""
},
"sspbi": {
"target_site": "T^GTACA",
"name": "SspBI",
"suppliers": [],
"source": "B. Frey",
"references": [
"Frey, B., Unpublished observations."
],
"prototype": "Bsp1407I",
"organism": "Streptomyces species",
"methylation_site": ""
},
"sexbi": {
"target_site": "CCGC^GG",
"name": "SexBI",
"suppliers": [],
"source": "J. Yim",
"references": [
"Bae, M., Park, J., Hwang, H.-Y., Yim, J., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces exfoliatus",
"methylation_site": ""
},
"halii": {
"target_site": "CTGCA^G",
"name": "HalII",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Verhosina, V.A., Vinogradova, T.P., (1990) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 1, pp. 35-37."
],
"prototype": "PstI",
"organism": "Hafnia alvei B6",
"methylation_site": ""
},
"eco67i": {
"target_site": "CCWGG",
"name": "Eco67I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Escherichia coli RFL67",
"methylation_site": ""
},
"smimi": {
"target_site": "CAYNN^NNRTG",
"name": "SmiMI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Najakshina, T.N., Gushina, E.N., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "MslI",
"organism": "Sphingobacterium mizutae M",
"methylation_site": ""
},
"uba1173i": {
"target_site": "GGATCC",
"name": "Uba1173I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1173",
"methylation_site": ""
},
"srli": {
"target_site": "G^CCGGC",
"name": "SrlI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Pristas, P., Fliegerova, K., Javorsky, P., (1998) Lett. Appl. Microbiol., vol. 27, pp. 83-85."
],
"prototype": "NaeI",
"organism": "Selenomonas ruminantium subsp. lactilytica 84",
"methylation_site": ""
},
"sau42i": {
"target_site": "?",
"name": "Sau42I",
"suppliers": [],
"source": "D.C. Coleman",
"references": [
"Coleman, D.C., Carroll, D., Dempsey, R.M., Sears, L.E., Kong, H., Unpublished observations."
],
"prototype": "",
"organism": "Staphylococcus aureus 42CR3-L",
"methylation_site": "?(6)"
},
"faundi": {
"target_site": "CA^TATG",
"name": "FauNDI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shevchenko, A.V., Belichenko, O.A., Myakisheva, T.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NdeI",
"organism": "Flavobacterium aquatile ND",
"methylation_site": ""
},
"tsti": {
"target_site": "(8/13)CACNNNNNNTCC(12/7)",
"name": "TstI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Jurenaite-Urbanaviciene, S., Serksnaite, J., Kriukiene, E., Giedriene, J., Venclovas, C., Lubys, A., (2007) Proc. Natl. Acad. Sci. U. S. A., vol. 104, pp. 10358-10363.",
"Maneliene, Z., Unpublished observations.",
"Nekrasiene, D., Norgeliene, D., Capskaja, L., Kiuduliene, L., Vitkute, J., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Thermus scotoductus RFL1",
"methylation_site": "?(6)"
},
"bst30i": {
"target_site": "CCTNAGG",
"name": "Bst30I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Serov, G.D., Tereschenko, T.A., Puchkova, L.I., Repin, V.E., Unpublished observations."
],
"prototype": "SauI",
"organism": "Bacillus stearothermophilus 30",
"methylation_site": ""
},
"mbii": {
"target_site": "CCGCTC(-3/-3)",
"name": "MbiI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Kazlauskiene, R., Trinkunaite, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BsrBI",
"organism": "Moraxella bovis Fr1-022",
"methylation_site": ""
},
"hgiciii": {
"target_site": "G^TCGAC",
"name": "HgiCIII",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141."
],
"prototype": "SalI",
"organism": "Herpetosiphon giganteus Hpg9",
"methylation_site": ""
},
"lladii": {
"target_site": "GCNGC",
"name": "LlaDII",
"suppliers": [],
"source": "J. Josephsen",
"references": [
"Josephsen, J., Nyengaard, N.R., Vogensen, F.K., Madsen, A., International Patent Office, 1996.",
"Madsen, A., Josephsen, J., (1998) Appl. Environ. Microbiol., vol. 64, pp. 2424-2431."
],
"prototype": "Fnu4HI",
"organism": "Lactococcus lactis W39",
"methylation_site": "?(5)"
},
"nlai": {
"target_site": "GGCC",
"name": "NlaI",
"suppliers": [],
"source": "NRCC 2118",
"references": [
"Qiang, B.-Q., Schildkraut, I., (1986) Nucleic Acids Res., vol. 14, pp. 1991-1999."
],
"prototype": "HaeIII",
"organism": "Neisseria lactamica",
"methylation_site": ""
},
"sphi": {
"target_site": "GCATG^C",
"name": "SphI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)"
],
"source": "NRRL B-3559",
"references": [
"Bhattacharya, S.K., Dubey, A.K., (1994) Biotechnol. Appl. Biochem., vol. 20, pp. 141-146.",
"Fuchs, L.Y., Covarrubias, L., Escalante, L., Sanchez, S., Bolivar, F., (1980) Gene, vol. 10, pp. 39-46.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32."
],
"prototype": "",
"organism": "Streptomyces phaeochromogenes",
"methylation_site": ""
},
"uba1289i": {
"target_site": "CCTNNNNNAGG",
"name": "Uba1289I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoNI",
"organism": "Unidentified bacterium RFL1289",
"methylation_site": ""
},
"hpyf2i": {
"target_site": "CTRYAG",
"name": "HpyF2I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "SfeI",
"organism": "Helicobacter pylori RFL2",
"methylation_site": ""
},
"btu34ii": {
"target_site": "RGCGCY",
"name": "Btu34II",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Burtseva, L.I., Burlak, V.A., Trusova, S.I., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus thuringiensis 34",
"methylation_site": ""
},
"afa24ri": {
"target_site": "GCCGGC",
"name": "Afa24RI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Acidiphilium facilis 24R",
"methylation_site": ""
},
"sblci": {
"target_site": "CCWWGG",
"name": "SblCI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Mise, K., Unpublished observations."
],
"prototype": "StyI",
"organism": "Salmonella blockley YY242",
"methylation_site": ""
},
"uban8i": {
"target_site": "GGCC",
"name": "UbaN8I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium TOT-02, Nicaragua",
"methylation_site": ""
},
"pde12i": {
"target_site": "G^GNCC",
"name": "Pde12I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Rechkunova, N.I., Novozhilova, M.I., Semenchenko, G.V., Galimdayeva, R.S., Soviet Patent Office, 1989."
],
"prototype": "AsuI",
"organism": "Paracoccus denitrificans 12",
"methylation_site": ""
},
"uba1243i": {
"target_site": "CCWGG",
"name": "Uba1243I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1243",
"methylation_site": ""
},
"bcesiii": {
"target_site": "ACGGC(12/14)",
"name": "BceSIII",
"suppliers": [],
"source": "ATCC 10987",
"references": [
"Fomenkov, A., Xu, S.-Y., Unpublished observations.",
"Rasko, D.A., Ravel, J., Okstad, O.A., Helgason, E., Cer, R.Z., Jiang, L., Shores, K.A., Fouts, D.E., Tourasse, N.J., Angiuoli, S.V., Kolonay, J., Nelson, W.C., Kolsto, A.-B., Fraser, C.M., Read, T.D., (2004) Nucleic Acids Res., vol. 32, pp. 977-988."
],
"prototype": "BcefI",
"organism": "Bacillus cereus",
"methylation_site": ""
},
"smaaii": {
"target_site": "GACNNNGTC",
"name": "SmaAII",
"suppliers": [],
"source": "UTEX 2342",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "Tth111I",
"organism": "Spirulina maxima",
"methylation_site": ""
},
"mnniv": {
"target_site": "GCGC",
"name": "MnnIV",
"suppliers": [],
"source": "ATCC 17954",
"references": [
"Hanberg, F., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HhaI",
"organism": "Moraxella nonliquefaciens",
"methylation_site": ""
},
"paehi": {
"target_site": "GRGCY^C",
"name": "PaeHI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravetz, A.N., Tarutina, Z.E., Solonin, A.S., (1991) Nucleic Acids Res., vol. 19, pp. 4781."
],
"prototype": "HgiJII",
"organism": "Pseudomonas aeruginosa 4148",
"methylation_site": ""
},
"novi": {
"target_site": "?",
"name": "NovI",
"suppliers": [],
"source": "NRCC 31020",
"references": [
"Visentin, L.P., Watson, R.J., Martin, S., Zuker, M., Unpublished observations."
],
"prototype": "",
"organism": "Neisseria ovis",
"methylation_site": ""
},
"bsp1407i": {
"target_site": "T^GTACA",
"name": "Bsp1407I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Petrusyte, M., Rudokas, K., Maneliene, Z., Kiuduliene, E., Butkus, V., Janulaitis, A., (1993) Nucleic Acids Res., vol. 21, pp. 2514."
],
"prototype": "",
"organism": "Bacillus stearothermophilus RFL1407",
"methylation_site": ""
},
"poli": {
"target_site": "GGWCC",
"name": "PolI",
"suppliers": [],
"source": "Viikki 13",
"references": [
"Mattila, P., Pitkanen, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Pseudomonas oleovorans",
"methylation_site": ""
},
"vha464i": {
"target_site": "C^TTAAG",
"name": "Vha464I",
"suppliers": [
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Kolykhalov, A.A., Repin, V.E., Fish, A.M., Rechkunova, N.I., Degtyarev, S.Kh., (1991) Sib. Biol. J., vol. 19, pp. 60-61.",
"Repin, V.E., Degtyarev, S.K., Rechkunova, N.I., Krivopalova, G.N., Vorodeva, T.I., Fish, A.M., Russian Patent Office, 1993."
],
"prototype": "AflII",
"organism": "Vibrio harveyi 464",
"methylation_site": ""
},
"eco105i": {
"target_site": "TAC^GTA",
"name": "Eco105I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Butkus, V., Maneliene, Z., Petrusyte, M., Unpublished observations."
],
"prototype": "SnaBI",
"organism": "Escherichia coli RFL105",
"methylation_site": ""
},
"m.dcai": {
"target_site": "?",
"name": "M.DcaI",
"suppliers": [],
"source": "R. Cella",
"references": [
"Bernacchia, G., Primo, A., Giorgetti, L., Pitto, L., Cella, R., (1998) Plant J., vol. 13, pp. 317-329."
],
"prototype": "",
"organism": "Daucus carota",
"methylation_site": "?(5)"
},
"uba1449i": {
"target_site": "GGCC",
"name": "Uba1449I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1449",
"methylation_site": ""
},
"asp22i": {
"target_site": "RGATCY",
"name": "Asp22I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Acidiphilium species 22",
"methylation_site": ""
},
"eco51ii": {
"target_site": "CCNGG",
"name": "Eco51II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Escherichia coli RFL51",
"methylation_site": ""
},
"btu36i": {
"target_site": "GATC",
"name": "Btu36I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Burtseva, L.I., Burlak, V.A., Trusova, S.I., (1991) Mol. Gen. Mikrobiol. Virusol., vol. 11, pp. 20-22."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis 36",
"methylation_site": ""
},
"uba1119i": {
"target_site": "CTGCAG",
"name": "Uba1119I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1119",
"methylation_site": ""
},
"eco24i": {
"target_site": "GRGCY^C",
"name": "Eco24I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Unpublished observations.",
"Naureckiene, S., Bitinaite, J., Vaitkevicius, D., Menkevicius, S., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Escherichia coli RFL24",
"methylation_site": ""
},
"spapiv": {
"target_site": "AAGCTT",
"name": "SpaPIV",
"suppliers": [],
"source": "D. Borthakur",
"references": [
"Tragut, V., Xiao, J., Bylina, E.J., Borthakur, D., (1995) J. Appl. Phycol., vol. 7, pp. 561-564."
],
"prototype": "HindIII",
"organism": "Spirulina platensis strain pacifica",
"methylation_site": ""
},
"bstv2i": {
"target_site": "GAAGAC(2/6)",
"name": "BstV2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Sinichkina, S.A., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus stearothermophilus V2",
"methylation_site": ""
},
"ecor5i": {
"target_site": "?",
"name": "EcoR5I",
"suppliers": [],
"source": "ATCC 35324",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR5",
"methylation_site": "?(6)"
},
"aquii": {
"target_site": "GCCGNAC(20/18)",
"name": "AquII",
"suppliers": [],
"source": "PCC 7002",
"references": [
"Morgan, R.D., Dwinell, E.A., Bhatia, T.K., Lang, E.M., Luyten, Y.A., (2009) Nucleic Acids Res., vol. 37, pp. 5208-5221."
],
"prototype": "",
"organism": "Agmenellum quadruplicatum PR-6",
"methylation_site": ""
},
"xmni": {
"target_site": "GAANN^NNTTC",
"name": "XmnI",
"suppliers": [
"New England Biolabs (1/11)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)"
],
"source": "ATCC 49764",
"references": [
"Lin, B.-C., Chien, M.-C., Lou, S.-Y., (1980) Nucleic Acids Res., vol. 8, pp. 6189-6198.",
"Nwankwo, D.O., Lynch, J.J., Moran, L.S., Fomenkov, A., Slatko, B.E., (1996) Gene, vol. 173, pp. 121-127.",
"Qiang, B.-Q., Schildkraut, I., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Xanthomonas manihotis 7AS1",
"methylation_site": ""
},
"bscki": {
"target_site": "GAAGAC",
"name": "BscKI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "BbvII",
"organism": "Bacillus species 217",
"methylation_site": ""
},
"aquiv": {
"target_site": "GRGGAAG(19/17)",
"name": "AquIV",
"suppliers": [],
"source": "PCC 7002",
"references": [
"Morgan, R.D., Dwinell, E.A., Unpublished observations."
],
"prototype": "",
"organism": "Agmenellum quadruplicatum PR-6",
"methylation_site": ""
},
"rma485i": {
"target_site": "CTAG",
"name": "Rma485I",
"suppliers": [],
"source": "ITI 485",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"spapii": {
"target_site": "CGATCG",
"name": "SpaPII",
"suppliers": [],
"source": "D. Borthakur",
"references": [
"Tragut, V., Xiao, J., Bylina, E.J., Borthakur, D., (1995) J. Appl. Phycol., vol. 7, pp. 561-564."
],
"prototype": "PvuI",
"organism": "Spirulina platensis strain pacifica",
"methylation_site": ""
},
"mph1103i": {
"target_site": "ATGCA^T",
"name": "Mph1103I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Maneliene, Z., Capskaya, L., Kiuduliene, L., Butkus, V., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Moraxella phenylpyruvica RFL1103",
"methylation_site": ""
},
"pvuii": {
"target_site": "CAG^CTG",
"name": "PvuII",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 13315",
"references": [
"Butkus, V., Klimasauskas, S., Petrauskiene, L., Maneliene, Z., Lebionka, A., Janulaitis, A., (1987) Biochim. Biophys. Acta, vol. 909, pp. 201-207.",
"Gingeras, T.R., Greenough, L., Schildkraut, I., Roberts, R.J., (1981) Nucleic Acids Res., vol. 9, pp. 4525-4536.",
"Kyune, C., Shimonchitoshu, A., Japanese Patent Office, 2006.",
"Rice, M.R., Calvin-Koons, M.D., Blumenthal, R.M., (1995) FASEB J., vol. 9.",
"Tao, T., Walter, J., Brennan, K.J., Cotterman, M.M., Blumenthal, R.M., (1989) Nucleic Acids Res., vol. 17, pp. 4161-4175."
],
"prototype": "",
"organism": "Proteus vulgaris",
"methylation_site": "4(4)"
},
"bshdi": {
"target_site": "GGCC",
"name": "BshDI",
"suppliers": [],
"source": "WHO/CCBC 2117-2",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"bsmfi": {
"target_site": "GGGAC(10/14)",
"name": "BsmFI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "Z. Chen",
"references": [
"Chen, Z., Morgan, R., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Zhu, Z., Unpublished observations.",
"Zhu, Z., Xu, S.-Y., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus stearothermophilus F",
"methylation_site": "?(6)"
},
"csp68kiii": {
"target_site": "ATGCA^T",
"name": "Csp68KIII",
"suppliers": [],
"source": "ATCC 51142",
"references": [
"Soper, B.W., (1996) Diss. Abstr., vol. 56, pp. 3585B.",
"Welsh, E.A., Liberton, M., Stockel, J., Sato, H., Loh, T., Wang, C., Wollam, A., Fulton, R.S., Clifton, S.W., Jacobs, J.M., Aurora, R., Sherman, L.A., Smith, R.D., Wilson, R.K., Pakrasi, H.B., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Cyanothece species BH68K",
"methylation_site": ""
},
"hpyf30i": {
"target_site": "TCGA",
"name": "HpyF30I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Helicobacter pylori RFL30",
"methylation_site": ""
},
"ecls39i": {
"target_site": "CCWGG",
"name": "EclS39I",
"suppliers": [],
"source": "W. Piepersberg",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae",
"methylation_site": ""
},
"bspni": {
"target_site": "CC^WGG",
"name": "BspNI",
"suppliers": [],
"source": "CAMB 2677",
"references": [
"Saboohi, N., Malik, K., Sohail, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus species N",
"methylation_site": ""
},
"bstdei": {
"target_site": "C^TNAG",
"name": "BstDEI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Bacillus stearothermophilus DE",
"methylation_site": ""
},
"sceiii": {
"target_site": "G^CCGGC",
"name": "SceIII",
"suppliers": [],
"source": "H.P. Zassenhaus",
"references": [
"Ezekiel, U.R., Zassenhaus, P., (1994) Biochem. Biophys. Res. Commun., vol. 201, pp. 208-214."
],
"prototype": "NaeI",
"organism": "Saccharomyces cerevisiae",
"methylation_site": ""
},
"m.taei": {
"target_site": "?",
"name": "M.TaeI",
"suppliers": [],
"source": "B.F. Vanyushin",
"references": [
"Vlasova, T.I., Kirnos, M.D., Vanyushin, B.F., (1996) Biokhimiia, vol. 61, pp. 774-780."
],
"prototype": "",
"organism": "Triticum aestivum",
"methylation_site": "?(5)"
},
"srl65di": {
"target_site": "ATTAAT",
"name": "Srl65DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "VspI",
"organism": "Selenomonas ruminantium 65",
"methylation_site": ""
},
"bpa36ii": {
"target_site": "CTNAG",
"name": "Bpa36II",
"suppliers": [],
"source": "NEB 1564",
"references": [
"Pan, X.S., Morgan, R., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Bacillus pallidus",
"methylation_site": ""
},
"hpyf12ii": {
"target_site": "TCNGA",
"name": "HpyF12II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL12",
"methylation_site": ""
},
"pxyari": {
"target_site": "GATATC",
"name": "PxyARI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Mrazek, J., Piknova, M., Pristas, P., Kopecny, J., (2005) Anaerobe, vol. 11, pp. 280-284."
],
"prototype": "EcoRV",
"organism": "Pseudobutyrivibrio species AR 11",
"methylation_site": ""
},
"bsp1883i": {
"target_site": "?",
"name": "Bsp1883I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "",
"organism": "Bacillus sphaericus 1883",
"methylation_site": ""
},
"bbvii": {
"target_site": "GAAGAC(2/6)",
"name": "BbvII",
"suppliers": [],
"source": "V.M. Kramarov",
"references": [
"Bunina, Z.F., Kramarov, V.M., Mazanov, A.L., Pachkunov, D.M., Smolianinov, V.V., Matvienko, N.N., (1983) Bioorg. Khim., vol. 9, pp. 1578-1580.",
"Matvienko, N.I., Pachkunov, D.M., Kramarov, V.M., (1984) FEBS Lett., vol. 177, pp. 23-26."
],
"prototype": "",
"organism": "Bacillus brevis 80",
"methylation_site": ""
},
"eco48i": {
"target_site": "CTGCAG",
"name": "Eco48I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "PstI",
"organism": "Escherichia coli RFL48",
"methylation_site": ""
},
"sru4di": {
"target_site": "AT^TAAT",
"name": "Sru4DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Godany, A., Javorsky, P., (1994) Arch. Microbiol., vol. 161, pp. 439-441."
],
"prototype": "VspI",
"organism": "Selenomonas ruminantium",
"methylation_site": ""
},
"uba1093i": {
"target_site": "CCGCGG",
"name": "Uba1093I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1093",
"methylation_site": ""
},
"bsrgii": {
"target_site": "?",
"name": "BsrGII",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus GR75",
"methylation_site": ""
},
"aspbii": {
"target_site": "GGWCC",
"name": "AspBII",
"suppliers": [],
"source": "ATCC 29151",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaII",
"organism": "Anabaena species",
"methylation_site": ""
},
"bsp42i": {
"target_site": "?",
"name": "Bsp42I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "",
"organism": "Bacillus sphaericus SL42",
"methylation_site": ""
},
"bscwi": {
"target_site": "GGGAC",
"name": "BscWI",
"suppliers": [],
"source": "NEB 1395",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "FinI",
"organism": "Bacillus species 2142",
"methylation_site": ""
},
"bamgi": {
"target_site": "CAG^CTG",
"name": "BamGI",
"suppliers": [],
"source": "Vivantis 52",
"references": [
"Chee, H.Y., Unpublished observations."
],
"prototype": "PvuII",
"organism": "B.acetylicum KSG",
"methylation_site": ""
},
"sflhk7234i": {
"target_site": "CCWGG",
"name": "SflHK7234I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 7234",
"methylation_site": ""
},
"bpu95i": {
"target_site": "CG^CG",
"name": "Bpu95I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Bacillus pumilus 95",
"methylation_site": ""
},
"asp27hi": {
"target_site": "GAATGC(1/-1)",
"name": "Asp27HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Kobayashi, F., Dou, D., Nomura, Y., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "BsmI",
"organism": "Acidiphilium species 27H",
"methylation_site": ""
},
"eco201i": {
"target_site": "GGNCC",
"name": "Eco201I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Escherichia coli RFL201",
"methylation_site": ""
},
"m.kpn43816dam": {
"target_site": "GATC",
"name": "M.Kpn43816Dam",
"suppliers": [],
"source": "S. Clegg",
"references": [
"Mehling, J.S., Lavender, H., Clegg, S., (2007) FEMS Microbiol. Lett., vol. 268, pp. 187-193."
],
"prototype": "MboI",
"organism": "Klebsiella pneumoniae strain 43816",
"methylation_site": "2(6)"
},
"uba1216i": {
"target_site": "CTGCAG",
"name": "Uba1216I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1216",
"methylation_site": ""
},
"bsegi": {
"target_site": "GGATG(2/0)",
"name": "BseGI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Lazareviciute, L., Maneliene, Z., Kvietkiene, E., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "FokI",
"organism": "Bacillus stearothermophilus Vs 34-031",
"methylation_site": ""
},
"aocii": {
"target_site": "GDGCH^C",
"name": "AocII",
"suppliers": [],
"source": "CCAP 1403/9",
"references": [
"de Waard, A., Unpublished observations."
],
"prototype": "SduI",
"organism": "Anabaena species",
"methylation_site": ""
},
"esp10i": {
"target_site": "GGYRCC",
"name": "Esp10I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL10",
"methylation_site": ""
},
"blohi": {
"target_site": "R^GATCY",
"name": "BloHI",
"suppliers": [],
"source": "ATCC 15707",
"references": [
"Hartke, A., Benachour, A., Boutibonnes, P., Auffray, Y., (1996) Appl. Microbiol. Biotechnol., vol. 45, pp. 132-136."
],
"prototype": "XhoII",
"organism": "Bifidobacterium longum E194b",
"methylation_site": ""
},
"ameii": {
"target_site": "GCCGGC",
"name": "AmeII",
"suppliers": [],
"source": "ATCC 15280",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Aquaspirillum metamorphum",
"methylation_site": ""
},
"bfrbi": {
"target_site": "ATGCA^T",
"name": "BfrBI",
"suppliers": [],
"source": "H. Azeddoug",
"references": [
"Azeddoug, H., Reysset, G., Sebald, M., (1992) FEMS Microbiol. Lett., vol. 95, pp. 133-136.",
"Morgan, R.D., Unpublished observations."
],
"prototype": "AvaIII",
"organism": "Bacteroides fragilis BE3",
"methylation_site": ""
},
"hin1056ii": {
"target_site": "?",
"name": "Hin1056II",
"suppliers": [],
"source": "J. Stuy",
"references": [
"Olson, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Haemophilus influenzae 1056",
"methylation_site": ""
},
"ecoicri": {
"target_site": "GAG^CTC",
"name": "EcoICRI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)"
],
"source": "ICR 0020",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335.",
"Yankovsky, N.K., Fonstein, M.Yu., Lashina, S.Yu., Bukanov, N.O., Yakubovich, N.V., Ermakova, L.M., Rebentish, B.A., Janulaitis, A., Debabov, V.G., (1989) Gene, vol. 81, pp. 203-210."
],
"prototype": "SacI",
"organism": "Escherichia coli 2bT",
"methylation_site": ""
},
"bshi": {
"target_site": "GG^CC",
"name": "BshI",
"suppliers": [],
"source": "WHO/CCBC 1593",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"cjenii": {
"target_site": "GAGNNNNNGT",
"name": "CjeNII",
"suppliers": [],
"source": "NCTC 11168",
"references": [
"Parkhill, J. et al., (2000) Nature, vol. 403, pp. 665-668.",
"Vitor, J., Vital, J., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Campylobacter jejuni 11168",
"methylation_site": "?(6)"
},
"nhaxi": {
"target_site": "CAAGRAG",
"name": "NhaXI",
"suppliers": [],
"source": "P. Richardson",
"references": [
"Morgan, R.D., Unpublished observations.",
"Starkenburg, S.R., Larimer, F.W., Stein, L.Y., Klotz, M.G., Chain, P.S.G., Sayavedra-Soto, L.A., Poret-Peterson, A.T., Gentry, M.E., Arp, D.J., Ward, B., Bottomley, P.J., (2008) Appl. Environ. Microbiol., vol. 74, pp. 2852-2863.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Nitrobacter hamburgensis X14",
"methylation_site": "?(6)"
},
"bco27i": {
"target_site": "C^CGG",
"name": "Bco27I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "HpaII",
"organism": "Bacillus coagulans 27",
"methylation_site": ""
},
"esp21i": {
"target_site": "GGYRCC",
"name": "Esp21I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL21",
"methylation_site": ""
},
"hpyf2ii": {
"target_site": "GANTC",
"name": "HpyF2II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HinfI",
"organism": "Helicobacter pylori RFL2",
"methylation_site": ""
},
"uba58i": {
"target_site": "GAATTC",
"name": "Uba58I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Unidentified bacterium RFL58",
"methylation_site": ""
},
"styltiii": {
"target_site": "GAGNNNNNNRTAYG",
"name": "StyLTIII",
"suppliers": [],
"source": "C. Colson",
"references": [
"Nagaraja, V., Shepherd, J.C.W., Bickle, T.A., (1985) Nature, vol. 316, pp. 371-372.",
"Nagaraja, V., Shepherd, J.C.W., Pripfl, T., Bickle, T.A., (1985) J. Mol. Biol., vol. 182, pp. 579-587."
],
"prototype": "",
"organism": "Salmonella typhimurium LT7",
"methylation_site": "2(6),-4(6)"
},
"hpyf52iii": {
"target_site": "GTAC",
"name": "HpyF52III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL52",
"methylation_site": ""
},
"bpri": {
"target_site": "?",
"name": "BprI",
"suppliers": [],
"source": "IFO 12128",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "",
"organism": "Brevibacterium protophormiae",
"methylation_site": ""
},
"bse634i": {
"target_site": "R^CCGGY",
"name": "Bse634I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Grazulis, S., Deibert, M., Rimseliene, R., Skirgaila, R., Sasnauskas, G., Lagunavicius, A., Repin, V., Urbanke, C., Huber, R., Siksnys, V., (2002) Nucleic Acids Res., vol. 30, pp. 876-885.",
"Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D., Tereschenko, T., Chizikov, V.E., Andreeva, I., (1995) Gene, vol. 157, pp. 321-322."
],
"prototype": "Cfr10I",
"organism": "Bacillus species 634",
"methylation_site": "?(5)"
},
"clmi": {
"target_site": "GGCC",
"name": "ClmI",
"suppliers": [],
"source": "ATCC 15219",
"references": [
"Smith, J., Comb, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Caryophanon latum",
"methylation_site": ""
},
"dsav": {
"target_site": "^CCNGG",
"name": "DsaV",
"suppliers": [],
"source": "A.E. Walsby",
"references": [
"Gopal, J., Bhagwat, A.S., (1995) Nucleic Acids Res., vol. 23, pp. 29-35.",
"Gopal, J., Yebra, M.J., Bhagwat, A.S., (1994) Nucleic Acids Res., vol. 22, pp. 4482-4488.",
"Laue, F., Evans, L.R., Jarsch, M., Brown, N.L., Kessler, C., (1991) Gene, vol. 97, pp. 87-95.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009."
],
"prototype": "ScrFI",
"organism": "Dactylococcopsis salina",
"methylation_site": "2(5)"
},
"hpyf22i": {
"target_site": "ACNGT",
"name": "HpyF22I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL22",
"methylation_site": ""
},
"sauhpi": {
"target_site": "GCC^GGC",
"name": "SauHPI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Pristas, P., Godany, A., Sevcikova, B., Oktavcova, B., Farkasovska, J., (1992) Nucleic Acids Res., vol. 20, pp. 4364."
],
"prototype": "NaeI",
"organism": "Streptomyces aureofaciens R8/26",
"methylation_site": ""
},
"bseyi": {
"target_site": "CCCAGC(-5/-1)",
"name": "BseYI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1396",
"references": [
"Bhatia, T., Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nkenfou, C., Unpublished observations.",
"Nkenfou, C., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species 2521",
"methylation_site": ""
},
"vpakutbi": {
"target_site": "GGNCC",
"name": "VpaKutBI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AsuI",
"organism": "Vibrio parahaemolyticus TVP1760",
"methylation_site": ""
},
"pmii": {
"target_site": "?",
"name": "PmiI",
"suppliers": [],
"source": "N.L. Bakh",
"references": [
"Bakh, N.L., Tsvetkova, N.V., Semina, I.E., Tarasov, A.P., Mileikovskaya, M.M., Gruber, I.M., Polyachenko, V.M., Romanenko, E.E., (1985) Antibiot. Med. Biotekhnol., vol. 30, pp. 342-344."
],
"prototype": "",
"organism": "Proteus mirabilis",
"methylation_site": ""
},
"snabi": {
"target_site": "TAC^GTA",
"name": "SnaBI",
"suppliers": [
"Minotech Biotechnology (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Promega Corporation (7/10)"
],
"source": "ATCC 15291",
"references": [
"Borsetti, R., Grandoni, R., Schildkraut, I., Unpublished observations.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Stickel, S.K., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Sphaerotilus natans",
"methylation_site": "3(4)"
},
"vpak19bi": {
"target_site": "GGNCC",
"name": "VpaK19BI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AsuI",
"organism": "Vibrio parahaemolyticus Matsuoka",
"methylation_site": ""
},
"bsspi": {
"target_site": "?",
"name": "BssPI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus P1",
"methylation_site": ""
},
"hgici": {
"target_site": "G^GYRCC",
"name": "HgiCI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Erdmann, D., Dusterhoft, A., Kroger, M., (1991) Eur. J. Biochem., vol. 202, pp. 1247-1256.",
"Kroger, M., Hobom, G., Schutte, H., Mayer, H., (1984) Nucleic Acids Res., vol. 12, pp. 3127-3141.",
"Puzio, P., Blau, A., Plesch, G., Kamlage, B., Looser, R., Schmitz, O., Wendel, B., European Patent Office, 2009.",
"Schildkraut, I., Lynch, J., Morgan, R., (1987) Nucleic Acids Res., vol. 15, pp. 5492."
],
"prototype": "",
"organism": "Herpetosiphon giganteus Hpg9",
"methylation_site": "5(5)"
},
"bsp1260i": {
"target_site": "GGWCC",
"name": "Bsp1260I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus sphaericus RFL1260",
"methylation_site": ""
},
"bfuaii": {
"target_site": "GCATG^C",
"name": "BfuAII",
"suppliers": [],
"source": "NEB 1292",
"references": [
"O'Loane, D., Morgan, R.D., Unpublished observations."
],
"prototype": "SphI",
"organism": "Bacillus fusiformis 1083",
"methylation_site": ""
},
"bsp73i": {
"target_site": "CCNGG",
"name": "Bsp73I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Bacillus species RFL73",
"methylation_site": ""
},
"dsai": {
"target_site": "C^CRYGG",
"name": "DsaI",
"suppliers": [],
"source": "A.E. Walsby",
"references": [
"Laue, F., Evans, L.R., Jarsch, M., Brown, N.L., Kessler, C., (1991) Gene, vol. 97, pp. 87-95."
],
"prototype": "",
"organism": "Dactylococcopsis salina",
"methylation_site": ""
},
"egei": {
"target_site": "GGC^GCC",
"name": "EgeI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Myakisheva, T.V., Kileva, E.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "NarI",
"organism": "Enterobacter gergoviae NA",
"methylation_site": ""
},
"kpn30i": {
"target_site": "GCGCGC",
"name": "Kpn30I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Klebsiella pneumoniae RFL30",
"methylation_site": ""
},
"bsarii": {
"target_site": "?",
"name": "BsaRII",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus stearothermophilus CPW8",
"methylation_site": ""
},
"apii": {
"target_site": "CTGCA^G",
"name": "ApiI",
"suppliers": [],
"source": "CAMB 2604",
"references": [
"Choudhry, S., (1999) Proc. Pakistan Acad. Sci., vol. 36, pp. 165-171."
],
"prototype": "PstI",
"organism": "Arthrobacter picolinophilus",
"methylation_site": ""
},
"uba1209i": {
"target_site": "GGCC",
"name": "Uba1209I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1209",
"methylation_site": ""
},
"bmgt120i": {
"target_site": "GG^NCC",
"name": "BmgT120I",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "Takara T120",
"references": [
"Sawaragi, H., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Bacillus megalosporus T120",
"methylation_site": ""
},
"mspb6i": {
"target_site": "?",
"name": "MspB6I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Zernov, Y.P., Rechkunova, N.I., Degtyarev, S.Kh., (1990) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 17-18."
],
"prototype": "",
"organism": "Moraxella species B6",
"methylation_site": ""
},
"assi": {
"target_site": "AGT^ACT",
"name": "AssI",
"suppliers": [
"Bangalore Genei (1/11)"
],
"source": "P. Babu",
"references": [
"Suma, M.A., Chandrashekaran, S., Babu, J., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Arthrobacter species",
"methylation_site": ""
},
"hpyf14ii": {
"target_site": "GTNNAC",
"name": "HpyF14II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MjaIV",
"organism": "Helicobacter pylori RFL14",
"methylation_site": ""
},
"sth302i": {
"target_site": "CC^WGG",
"name": "Sth302I",
"suppliers": [],
"source": "G. Guedon",
"references": [
"Morgan, R.D., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Streptococcus thermophilus CNRZ302",
"methylation_site": ""
},
"uba1238i": {
"target_site": "ATCGAT",
"name": "Uba1238I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1238",
"methylation_site": ""
},
"accb7i": {
"target_site": "CCANNNN^NTGG",
"name": "AccB7I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Repin, V.E., Rechkunova, N.I., Degtyarev, S.K., Verhosina, V.A., Vinogradova, T.P., (1990) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 1, pp. 35-37."
],
"prototype": "PflMI",
"organism": "Acinetobacter calcoaceticus B7",
"methylation_site": ""
},
"ecoo109i": {
"target_site": "RG^GNCCY",
"name": "EcoO109I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)"
],
"source": "I. Orskov",
"references": [
"Kita, K., Tsuda, J., Kato, T., Okamoto, K., Yanase, H., Tanaka, M., (1999) J. Bacteriol., vol. 181, pp. 6822-6827.",
"Kita, K., Tsuda, J., Nishigaki, R., (2001) Biosci. Biotechnol. Biochem., vol. 65, pp. 2512-2518.",
"Mise, K., Nakajima, K., (1985) Gene, vol. 36, pp. 363-367.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "DraII",
"organism": "Escherichia coli H709c",
"methylation_site": "5(5)"
},
"acs1373i": {
"target_site": "GTCGAC",
"name": "Acs1373I",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Hamafuku, H., Kotani, H., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "SalI",
"organism": "Acidobacterium capsulatum 1373",
"methylation_site": ""
},
"bscdi": {
"target_site": "CTGCAG",
"name": "BscDI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "PstI",
"organism": "Bacillus species E13",
"methylation_site": ""
},
"nmusi": {
"target_site": "GGNCC",
"name": "NmuSI",
"suppliers": [],
"source": "ATCC 19693",
"references": [
"Comb, D.G., Parker, P., Schildkraut, I., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Neisseria mucosa",
"methylation_site": ""
},
"pae41i": {
"target_site": "CTGCAG",
"name": "Pae41I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL41",
"methylation_site": ""
},
"pvu84ii": {
"target_site": "CAG^CTG",
"name": "Pvu84II",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Proteus vulgaris 84",
"methylation_site": ""
},
"tsp273i": {
"target_site": "GATATC",
"name": "Tsp273I",
"suppliers": [],
"source": "ITI 273",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Thermus species",
"methylation_site": ""
},
"bspi": {
"target_site": "GATC",
"name": "BspI",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Leung, S.M., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"acei": {
"target_site": "G^CWGC",
"name": "AceI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Rahman, H., Affram, C., Unpublished observations."
],
"prototype": "TseI",
"organism": "Anabaena cedrorum",
"methylation_site": ""
},
"yenai": {
"target_site": "CTGCAG",
"name": "YenAI",
"suppliers": [],
"source": "T. Maruyama",
"references": [
"Miyahara, M., Maruyama, T., Wake, A., Mise, K., (1988) Appl. Environ. Microbiol., vol. 54, pp. 577-580."
],
"prototype": "PstI",
"organism": "Yersinia enterocolitica O8 WA",
"methylation_site": ""
},
"eco205i": {
"target_site": "GGTCTC",
"name": "Eco205I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL205",
"methylation_site": ""
},
"sth117i": {
"target_site": "CC^WGG",
"name": "Sth117I",
"suppliers": [],
"source": "G.A. Somkuti",
"references": [
"Hill, C., (1993) FEMS Microbiol. Rev., vol. 12, pp. 87-108.",
"Solaiman, D.K.Y., Somkuti, G.A., (1991) FEMS Microbiol. Lett., vol. 80, pp. 75-80."
],
"prototype": "EcoRII",
"organism": "Streptococcus thermophilus ST117",
"methylation_site": ""
},
"bsth9i": {
"target_site": "GGATC(4/5)",
"name": "BstH9I",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Polisson, C., Morgan, R., Unpublished observations."
],
"prototype": "BinI",
"organism": "Bacillus stearothermophilus H9",
"methylation_site": ""
},
"uban2i": {
"target_site": "GGCC",
"name": "UbaN2I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium OQ3-03, Nicaragua",
"methylation_site": ""
},
"hpyf30ii": {
"target_site": "CTNAG",
"name": "HpyF30II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL30",
"methylation_site": ""
},
"mnoiii": {
"target_site": "GATC",
"name": "MnoIII",
"suppliers": [],
"source": "ATCC 19975",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Moraxella nonliquefaciens",
"methylation_site": ""
},
"bstz15i": {
"target_site": "GDGCHC",
"name": "BstZ15I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "SduI",
"organism": "Bacillus stearothermophilus BD102",
"methylation_site": ""
},
"bspssi": {
"target_site": "?",
"name": "BspSSI",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "",
"organism": "Bacillus sphaericus SSII-1",
"methylation_site": ""
},
"bstc8i": {
"target_site": "GCN^NGC",
"name": "BstC8I",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Mikhnenkova, N.A., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Cac8I",
"organism": "Bacillus stearothermophilus C8",
"methylation_site": ""
},
"uba1126i": {
"target_site": "CCGCGG",
"name": "Uba1126I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1126",
"methylation_site": ""
},
"bstm6i": {
"target_site": "CC^WGG",
"name": "BstM6I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Shapovalova, N.I., Ivanov, L.Yu., Matvienko, N.I., (1992) Bioorg. Khim., vol. 18, pp. 1186-1189."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus M6",
"methylation_site": ""
},
"uba1440i": {
"target_site": "CYCGRG",
"name": "Uba1440I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Unidentified bacterium RFL1440",
"methylation_site": ""
},
"eco80i": {
"target_site": "CCNGG",
"name": "Eco80I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Escherichia coli RFL80",
"methylation_site": ""
},
"bst38i": {
"target_site": "CC^WGG",
"name": "Bst38I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E., (1993) Bioorg. Khim., vol. 19, pp. 583-585."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus 38",
"methylation_site": ""
},
"bspani": {
"target_site": "GG^CC",
"name": "BspANI",
"suppliers": [],
"source": "P.M. Skowron",
"references": [
"Skowron, P.M., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sp. AN",
"methylation_site": ""
},
"nhei": {
"target_site": "G^CTAGC",
"name": "NheI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 25999",
"references": [
"Comb, D.G., Grandoni, R., Schildkraut, I., Unpublished observations.",
"Xu, S.-Y., Xiao, J.-P., European Patent Office, 2001."
],
"prototype": "",
"organism": "Neisseria mucosa",
"methylation_site": ""
},
"ava458i": {
"target_site": "YGGCCR",
"name": "Ava458I",
"suppliers": [],
"source": "M.I. Mendzhul",
"references": [
"Mendzhul, M.I., Moshinsky, I.D., Syrchin, S.A., (1999) Mikrobiol. Zh., vol. 61, pp. 10-14."
],
"prototype": "CfrI",
"organism": "Anabaena variabilis CALU 458",
"methylation_site": ""
},
"bsu15i": {
"target_site": "AT^CGAT",
"name": "Bsu15I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Janulaitis, A., Lubys, A., Mitkaite, G., Bitinaite, J., Maneliene, Z., Butkus, V., Unpublished observations.",
"Prichodko, G.G., Petrov, N.A., Repin, V.E., Degtyarev, S.K., (1988) Izv. Sib. Otd. Akad. Nauk SSSR, vol. 14, pp. 108-109."
],
"prototype": "ClaI",
"organism": "Bacillus subtilis 15",
"methylation_site": "5(6)"
},
"cfr6i": {
"target_site": "CAG^CTG",
"name": "Cfr6I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Butkus, V., Klimasauskas, S., Petrauskiene, L., Maneliene, Z., Lebionka, A., Janulaitis, A., (1987) Biochim. Biophys. Acta, vol. 909, pp. 201-207.",
"Janulaitis, A., Stakenas, P.S., Bitinaite, J.B., Jaskeleviciene, B.P., (1983) Dokl. Akad. Nauk., vol. 271, pp. 483-485.",
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129."
],
"prototype": "PvuII",
"organism": "Citrobacter freundii RFL6",
"methylation_site": "4(4)"
},
"ttni": {
"target_site": "GGCC",
"name": "TtnI",
"suppliers": [],
"source": "W. Siegelman",
"references": [
"Streips, U., Golemboski, B., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Tolypothrix tenuis",
"methylation_site": ""
},
"uba1316i": {
"target_site": "GGTCTC",
"name": "Uba1316I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Unidentified bacterium RFL1316",
"methylation_site": ""
},
"asptii": {
"target_site": "GGATCC",
"name": "AspTII",
"suppliers": [],
"source": "C.P. Wolk",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Anabaena species TA 1",
"methylation_site": ""
},
"sspd5ii": {
"target_site": "ATGCA^T",
"name": "SspD5II",
"suppliers": [],
"source": "N. Matvienko",
"references": [
"Shiryaev, S.A., Zheleznyakova, E.N., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (2000) Biokhimiia, vol. 65, pp. 553-561."
],
"prototype": "AvaIII",
"organism": "Staphylococcus species D5",
"methylation_site": ""
},
"cfr29i": {
"target_site": "CCWGG",
"name": "Cfr29I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL29",
"methylation_site": ""
},
"bsp102i": {
"target_site": "TTCGAA",
"name": "Bsp102I",
"suppliers": [],
"source": "ITI 102",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"bshci": {
"target_site": "GGCC",
"name": "BshCI",
"suppliers": [],
"source": "WHO/CCBC 2013-6",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"ppuai": {
"target_site": "C^GTACG",
"name": "PpuAI",
"suppliers": [],
"source": "P. Adams",
"references": [
"Nelson, M., Unpublished observations."
],
"prototype": "SplI",
"organism": "Pseudomonas putida A1",
"methylation_site": ""
},
"hgiki": {
"target_site": "?",
"name": "HgiKI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Herpetosiphon giganteus Hpg32",
"methylation_site": ""
},
"binsii": {
"target_site": "GGCGC^C",
"name": "BinSII",
"suppliers": [],
"source": "ATCC 15702",
"references": [
"Khosaka, T., Kiwaki, M., Rak, B., (1983) FEBS Lett., vol. 163, pp. 170-174.",
"Khosaka, T., Sakurai, T., Mutai, M., European Patent Office, 1981."
],
"prototype": "NarI",
"organism": "Bifidobacterium infantis S76e",
"methylation_site": ""
},
"bsu36i": {
"target_site": "CC^TNAGG",
"name": "Bsu36I",
"suppliers": [
"New England Biolabs (1/11)",
"Promega Corporation (7/10)"
],
"source": "B. Zhou",
"references": [
"Heiter, D., Lunnen, K., Wilson, G.G., US Patent Office, 2006.",
"Lunnen, K.D., Heiter, D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Zhou, B., Li, Q., (1987) Acta Biochim. Biophys. Sin., vol. 19, pp. 537-540."
],
"prototype": "SauI",
"organism": "Bacillus subtilis 36",
"methylation_site": ""
},
"mcai": {
"target_site": "CTCGAG",
"name": "McaI",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Kessler, C., Neumaier, P.S., Wolf, W., (1985) Gene, vol. 33, pp. 1-102."
],
"prototype": "XhoI",
"organism": "Micromonospora carbonacea",
"methylation_site": ""
},
"csp231i": {
"target_site": "AAGCTT",
"name": "Csp231I",
"suppliers": [],
"source": "T. Kaczorowski",
"references": [
"Mruk, I., Kaczorowski, T., (2007) Appl. Environ. Microbiol., vol. 73, pp. 4286-4293."
],
"prototype": "HindIII",
"organism": "Citrobacter sp. RFL231",
"methylation_site": ""
},
"bsp64i": {
"target_site": "GATC",
"name": "Bsp64I",
"suppliers": [],
"source": "S.Y. Ye",
"references": [
"Yan, P.-F., Ye, S.-Y., Wang, P.-Z., Li, Q.-L., Lu, Y.-Y., Zhou, B., (1982) Acta Biochim. Biophys. Sin., vol. 14, pp. 151-158."
],
"prototype": "MboI",
"organism": "Bacillus sphaericus 64",
"methylation_site": ""
},
"bpuji": {
"target_site": "CCCGT",
"name": "BpuJI",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Sukackaite, R., Lagunavicius, A., Stankevicius, K., Urbanke, C., Venclovas, C., Siksnys, V., (2007) Nucleic Acids Res., vol. 35, pp. 2377-2389.",
"Vitkute, J., Trinkunaite, L., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BscGI",
"organism": "Bacillus pumilus RFL1458",
"methylation_site": ""
},
"nlaiv": {
"target_site": "GGN^NCC",
"name": "NlaIV",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NRCC 2118",
"references": [
"Lau, P.C.K., Forghani, F., Labbe, D., Bergeron, H., Brousseau, R., Holtke, H.J., (1994) Mol. Gen. Genet., vol. 243, pp. 24-31.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Qiang, B.-Q., Schildkraut, I., (1986) Nucleic Acids Res., vol. 14, pp. 1991-1999."
],
"prototype": "",
"organism": "Neisseria lactamica",
"methylation_site": "?(5)"
},
"hpyf38ii": {
"target_site": "TGCA",
"name": "HpyF38II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori RFL38",
"methylation_site": ""
},
"bli576i": {
"target_site": "ATCGAT",
"name": "Bli576I",
"suppliers": [],
"source": "C. Parini",
"references": [
"Parini, C., Fortina, M.G., (1995) FEMS Microbiol. Lett., vol. 132, pp. 285-289."
],
"prototype": "ClaI",
"organism": "Bacillus licheniformis 576",
"methylation_site": ""
},
"ple214i": {
"target_site": "GGCC",
"name": "Ple214I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Photobacterium leiognathi",
"methylation_site": ""
},
"bsbi": {
"target_site": "CAACAC(21/19)",
"name": "BsbI",
"suppliers": [],
"source": "NEB 686",
"references": [
"Morgan, R.D., Unpublished observations.",
"Zhou, B., Morgan, R., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"aniai": {
"target_site": "?",
"name": "AniAI",
"suppliers": [],
"source": "PCC 6301",
"references": [
"Szekeres, M., (1981) Virology, vol. 111, pp. 1-10."
],
"prototype": "",
"organism": "Anacystis nidulans",
"methylation_site": ""
},
"hpyf73iii": {
"target_site": "GGCC",
"name": "HpyF73III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL73",
"methylation_site": ""
},
"bst22i": {
"target_site": "CCNNNNN^NNGG",
"name": "Bst22I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Ushakova, T.A., Puchkova, L.I., Radionenko, Y.V., Kuvshinov, V.N., Repin, V.E., (2003) Biotekhnologiya, vol. 0, pp. 11-15."
],
"prototype": "BsiYI",
"organism": "Bacillus stearothermophilus 22",
"methylation_site": ""
},
"uba1178i": {
"target_site": "GGCC",
"name": "Uba1178I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1178",
"methylation_site": ""
},
"bal3006i": {
"target_site": "GGCC",
"name": "Bal3006I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus alvei 3006",
"methylation_site": ""
},
"bsth2i": {
"target_site": "RGCGC^Y",
"name": "BstH2I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus stearothermophilus H2",
"methylation_site": ""
},
"csp68kii": {
"target_site": "TT^CGAA",
"name": "Csp68KII",
"suppliers": [],
"source": "ATCC 51142",
"references": [
"Soper, B.W., (1996) Diss. Abstr., vol. 56, pp. 3585B.",
"Welsh, E.A., Liberton, M., Stockel, J., Sato, H., Loh, T., Wang, C., Wollam, A., Fulton, R.S., Clifton, S.W., Jacobs, J.M., Aurora, R., Sherman, L.A., Smith, R.D., Wilson, R.K., Pakrasi, H.B., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Cyanothece species BH68K",
"methylation_site": ""
},
"eco247i": {
"target_site": "GGTCTC",
"name": "Eco247I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL247",
"methylation_site": ""
},
"asp17hi": {
"target_site": "GTAC",
"name": "Asp17HI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Dou, D., Kobayashi, F., Hiraoka, N., Kishimoto, N., Tano, T., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Acidiphilium species 17H",
"methylation_site": ""
},
"bsp4i": {
"target_site": "ATCGAT",
"name": "Bsp4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species RFL4",
"methylation_site": ""
},
"eco1447i": {
"target_site": "TGANNNNNNNNTGCT",
"name": "Eco1447I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Ryu, J.-I., Unpublished observations."
],
"prototype": "EcoBI",
"organism": "Escherichia coli EC1447",
"methylation_site": ""
},
"msli": {
"target_site": "CAYNN^NNRTG",
"name": "MslI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 722",
"references": [
"Morgan, R.D., Unpublished observations.",
"Qiang, B.-Q., Wu, S., Kong, H., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Moraxella osloensis",
"methylation_site": ""
},
"cfri": {
"target_site": "Y^GGCCR",
"name": "CfrI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Stakenas, P., Jaskeleviciene, B., Lebedenko, E.N., Berlin, Y.A., (1980) Bioorg. Khim., vol. 6, pp. 1746-1748.",
"Janulaitis, A., Stakenas, P.S., Lebedenko, E.N., Berlin, Y.A., (1982) Nucleic Acids Res., vol. 10, pp. 6521-6530.",
"Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V., (1983) Mol. Biol. (Mosk), vol. 18, pp. 115-129.",
"Klimasauskas, S., Butkus, V., Janulaitis, A., (1987) Mol. Biol. (Mosk), vol. 21, pp. 87-92."
],
"prototype": "",
"organism": "Citrobacter freundii RFL2",
"methylation_site": "4(5)"
},
"bsiwi": {
"target_site": "C^GTACG",
"name": "BsiWI",
"suppliers": [
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "D. Clark",
"references": [
"Clark, D.R., Klein, S., Roberts, R.J., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nwankwo, D., Unpublished observations.",
"Zhu, Z., Unpublished observations.",
"Zhu, Z., Xu, S.-Y., Unpublished observations."
],
"prototype": "SplI",
"organism": "Bacillus species",
"methylation_site": ""
},
"acsi": {
"target_site": "R^AATTY",
"name": "AcsI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Kolyhalov, A.A., Rechkunova, N.I., Abdurashitov, M.A., (1992) Nucleic Acids Res., vol. 20, pp. 3789."
],
"prototype": "ApoI",
"organism": "Arthrobacter citreus 310",
"methylation_site": ""
},
"esabc4i": {
"target_site": "GG^CC",
"name": "EsaBC4I",
"suppliers": [],
"source": "R.D. Morgan",
"references": [
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "HaeIII",
"organism": "Environmental sample BC4",
"methylation_site": "?(4)"
},
"bfoi": {
"target_site": "RGCGC^Y",
"name": "BfoI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G345",
"references": [
"Vitkute, J., Lauciuniene, N., Lapcinskaja, S., Trinkunaite, L., Zakareviciene, L., Lubys, A., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeII",
"organism": "Bacillus fusiformis RFL1",
"methylation_site": ""
},
"vpak25i": {
"target_site": "GGNCC",
"name": "VpaK25I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Kotani, H., Mise, K., (1992) Gene, vol. 117, pp. 103-106."
],
"prototype": "AsuI",
"organism": "Vibrio parahaemolyticus 3010-61",
"methylation_site": ""
},
"mfei": {
"target_site": "C^AATTG",
"name": "MfeI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "N.F. Halden",
"references": [
"Halden, N.F., Wolf, J.B., Cross, S.L., Leonard, W.J., (1988) Clin. Res., vol. 36, pp. 404a.",
"Halden, N.F., Wolf, J.B., Leonard, W.J., (1989) Nucleic Acids Res., vol. 17, pp. 3491-3499.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Xu, S.-Y., Unpublished observations.",
"Zhu, Z., Blanchard, A., Xu, S.-Y., Guan, S., Wei, H., Zhang, P., Sun, D., Chan, S.-h., International Patent Office, 2009."
],
"prototype": "",
"organism": "Mycoplasma fermentans",
"methylation_site": "?(6)"
},
"m.mmudnmt1": {
"target_site": "?",
"name": "M.MmuDnmt1",
"suppliers": [],
"source": "T. Bestor",
"references": [
"Aguirre-Arteta, A.M., Grunewald, I., Cardoso, M.C., Leonhardt, H., (2000) Cell Growth Differ., vol. 11, pp. 551-559.",
"Bestor, T., Laudano, A., Mattaliano, R., Ingram, V., (1988) J. Mol. Biol., vol. 203, pp. 971-983.",
"Bestor, T.H., (1988) Gene, vol. 74, pp. 9-12.",
"Margot, J.B., Aguirre-Arteta, A.M., Di Giacco, B.V., Pradhan, S., Roberts, R.J., Cardoso, M.C., Leonhardt, H., (2000) J. Mol. Biol., vol. 297, pp. 293-300.",
"Mertineit, C., Yoder, J.A., Taketo, T., Laird, D.W., Trasler, J.M., Bestor, T.H., (1998) Development, vol. 125, pp. 889-897.",
"Yoder, J.A., Yen, R.-W.C., Vertino, P.M., Bestor, T.H., Baylin, S.B., (1996) J. Biol. Chem., vol. 271, pp. 31092-31097."
],
"prototype": "",
"organism": "Mus musculus",
"methylation_site": "?(5)"
},
"cliii": {
"target_site": "TGCGCA",
"name": "CliII",
"suppliers": [],
"source": "UTEX 2014",
"references": [
"Karreman, C., de Waard, A., Unpublished observations."
],
"prototype": "MstI",
"organism": "Cylindrospermum lichenforme",
"methylation_site": ""
},
"bssbi": {
"target_site": "GCGCGC",
"name": "BssBI",
"suppliers": [],
"source": "X.S. Pan",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Bacillus stearothermophilus BZ153",
"methylation_site": ""
},
"ospi": {
"target_site": "TTCGAA",
"name": "OspI",
"suppliers": [],
"source": "B.K. Hurlburt",
"references": [
"Hurlburt, B.K., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Oscillatoria splendida",
"methylation_site": ""
},
"uba1336i": {
"target_site": "GGCC",
"name": "Uba1336I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1336",
"methylation_site": ""
},
"sno506i": {
"target_site": "GGCCGAG",
"name": "Sno506I",
"suppliers": [],
"source": "DSM 506",
"references": [
"Dwinell, E.A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Starkeya novella",
"methylation_site": "?(6)"
},
"uba1280i": {
"target_site": "CCSGG",
"name": "Uba1280I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1280",
"methylation_site": ""
},
"bspj106i": {
"target_site": "GGTACC",
"name": "BspJ106I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Bacillus species RFL106",
"methylation_site": ""
},
"tspdti": {
"target_site": "ATGAA(11/9)",
"name": "TspDTI",
"suppliers": [
"Molecular Biology Resources - CHIMERx (1/11)",
"EURx Ltd. (1/11)"
],
"source": "P.M. Skowron",
"references": [
"Skowron, P.M., Majewski, J., Zylicz-Stachula, A., Rutkowska, S.M., Jaworowska, I., Harasimowicz-Slowinska, R.I., (2003) Nucleic Acids Res., vol. 31.",
"Skowron, P.M., Zylicz-Stachula, A., Unpublished observations."
],
"prototype": "",
"organism": "Thermus sp. DT",
"methylation_site": ""
},
"uba1121i": {
"target_site": "CCWGG",
"name": "Uba1121I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1121",
"methylation_site": ""
},
"bsu2413i": {
"target_site": "?",
"name": "Bsu2413I",
"suppliers": [],
"source": "A.R. Reddy",
"references": [
"Jutur, P.P., Hoti, S.L., Reddy, A.R., (2004) Mol. Biol. Rep., vol. 31, pp. 139-142."
],
"prototype": "",
"organism": "Bacillus subtilis 2413",
"methylation_site": ""
},
"suni": {
"target_site": "C^GTACG",
"name": "SunI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Wijdenbosch, M.M., Unpublished observations."
],
"prototype": "SplI",
"organism": "Synechococcus uniformis",
"methylation_site": ""
},
"acciii": {
"target_site": "T^CCGGA",
"name": "AccIII",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Promega Corporation (7/10)"
],
"source": "ATCC 49823",
"references": [
"Kita, K., Hiraoka, N., Oshima, A., Kadonishi, S., Obayashi, A., (1985) Nucleic Acids Res., vol. 13, pp. 8685-8694.",
"Sagawa, H., Ueno, H., Oshima, A., Kato, I., International Patent Office, 1997.",
"Sagawa, H., Ueno, H., Oshima, A., Kato, I., US Patent Office, 2004."
],
"prototype": "BspMII",
"organism": "Acinetobacter calcoaceticus",
"methylation_site": ""
},
"bsp151i": {
"target_site": "TTCGAA",
"name": "Bsp151I",
"suppliers": [],
"source": "ITI 151",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "AsuII",
"organism": "Bacillus species",
"methylation_site": ""
},
"psp3i": {
"target_site": "CAGCTG",
"name": "Psp3I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Pseudomonas species RFL3",
"methylation_site": ""
},
"uba1426i": {
"target_site": "CTTAAG",
"name": "Uba1426I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AflII",
"organism": "Unidentified bacterium RFL1426",
"methylation_site": ""
},
"bthai": {
"target_site": "G^GWCC",
"name": "BthAI",
"suppliers": [],
"source": "CAMB 2661",
"references": [
"Arif, A., Ehsan, A., Sohail, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus thuringiensis AII",
"methylation_site": ""
},
"bcesii": {
"target_site": "GGWCC",
"name": "BceSII",
"suppliers": [],
"source": "ATCC 10987",
"references": [
"Rasko, D.A., Ravel, J., Okstad, O.A., Helgason, E., Cer, R.Z., Jiang, L., Shores, K.A., Fouts, D.E., Tourasse, N.J., Angiuoli, S.V., Kolonay, J., Nelson, W.C., Kolsto, A.-B., Fraser, C.M., Read, T.D., (2004) Nucleic Acids Res., vol. 32, pp. 977-988.",
"Zheng, Y., Roberts, R.J., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus cereus",
"methylation_site": "?(5)"
},
"bsp1261i": {
"target_site": "GGCC",
"name": "Bsp1261I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus RFL1261",
"methylation_site": ""
},
"rroi": {
"target_site": "GTCGAC",
"name": "RroI",
"suppliers": [],
"source": "ATCC 4276",
"references": [
"Qiang, B.-Q., Schildkraut, I., Unpublished observations."
],
"prototype": "SalI",
"organism": "Rhodococcus rhodochrous",
"methylation_site": ""
},
"tcei": {
"target_site": "GAAGA",
"name": "TceI",
"suppliers": [],
"source": "W. Zillig",
"references": [
"McWilliam, P., Unpublished observations."
],
"prototype": "MboII",
"organism": "Thermococcus celer",
"methylation_site": ""
},
"vcho49i": {
"target_site": "AGTACT",
"name": "VchO49I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Mise, K., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 599-604."
],
"prototype": "ScaI",
"organism": "Vibrio cholerae 1154-74",
"methylation_site": ""
},
"sthai": {
"target_site": "GGTACC",
"name": "SthAI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "KpnI",
"organism": "Salmonella thompson YY17",
"methylation_site": ""
},
"omibii": {
"target_site": "GTMKAC",
"name": "OmiBII",
"suppliers": [],
"source": "NEB 971",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "AccI",
"organism": "Oceanospirillum minutulum",
"methylation_site": ""
},
"mmup2i": {
"target_site": "GATC",
"name": "MmuP2I",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2004) FEMS Microbiol. Lett., vol. 236, pp. 91-95."
],
"prototype": "MboI",
"organism": "Mitsuokella multiacida 4P208-58",
"methylation_site": ""
},
"hpyf49iv": {
"target_site": "GGCC",
"name": "HpyF49IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL49",
"methylation_site": ""
},
"bsp135i": {
"target_site": "GATC",
"name": "Bsp135I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus species RFL135",
"methylation_site": ""
},
"hpyf49ii": {
"target_site": "GTSAC",
"name": "HpyF49II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL49",
"methylation_site": ""
},
"smimbi": {
"target_site": "GATC",
"name": "SmiMBI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Degtyarev, S.Kh., (1998) Biol. Chem., vol. 379, pp. 573-574."
],
"prototype": "MboI",
"organism": "Streptococcus milleri MB",
"methylation_site": ""
},
"hpyav": {
"target_site": "CCTTC(6/5)",
"name": "HpyAV",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "D.E. Berg",
"references": [
"Chan, S.H., Opitz, L., Higgins, L., O'loane, D., Xu, S.-Y., (2010) PLoS ONE, vol. 5.",
"Higgins, L., Morgan, R.D., Kong, H., Unpublished observations.",
"Kleanthous, H., Garawi, A.A., Miller, C., Tomb, J.F., Oomen, R.P., Japanese Patent Office, 2001.",
"Kong, H., Sears, L., Unpublished observations.",
"Lin, L.-F., Kong, H., Unpublished observations.",
"Tomb, J.-F. et al., (1997) Nature, vol. 388, pp. 539-547.",
"Xu, S.-Y., Unpublished observations."
],
"prototype": "Hin4II",
"organism": "Helicobacter pylori 26695",
"methylation_site": "?(5)"
},
"sfl2ai": {
"target_site": "CCWGG",
"name": "Sfl2aI",
"suppliers": [],
"source": "L.I. Solodukhina",
"references": [
"Solodukhina, L.I., Korotayev, A.I., Solonin, A.S., Kuzmin, N.P., (1990) Genetika, vol. 26, pp. 1126-1128.",
"Solodukhina, L.I., Manuvakhova, M.S., Korotayev, A.I., (1989) Genetika, vol. 25, pp. 1571-1577."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 2a",
"methylation_site": ""
},
"fsei": {
"target_site": "GGCCGG^CC",
"name": "FseI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NRRL 18528",
"references": [
"Morgan, R.D., US Patent Office, 1996.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Nelson, J.M., Miceli, S.M., Lechevalier, M.P., Roberts, R.J., (1990) Nucleic Acids Res., vol. 18, pp. 2061-2064."
],
"prototype": "",
"organism": "Frankia species Eul1b",
"methylation_site": "?(5)"
},
"uba1172i": {
"target_site": "GGATCC",
"name": "Uba1172I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1172",
"methylation_site": ""
},
"pae2ki": {
"target_site": "A^GATCT",
"name": "Pae2kI",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Kravets, A.N., Pertsev, A.V., Tarutina, Z.Y., Krendelev, Y.D., Zakharova, M.V., Beletskaya, I.V., Solonin, A.S., (1998) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 14-17."
],
"prototype": "BglII",
"organism": "Pseudomonas aeruginosa 2k",
"methylation_site": ""
},
"faqi": {
"target_site": "GGGAC(10/14)",
"name": "FaqI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G263",
"references": [
"Vitkute, J., Mociskyte, S., Maneliene, Z., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "FinI",
"organism": "Flavobacterium aquatile RFL1",
"methylation_site": ""
},
"suli": {
"target_site": "GGCC",
"name": "SulI",
"suppliers": [],
"source": "W. Zillig",
"references": [
"McWilliam, P., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Sulfolobus acidocaldarius",
"methylation_site": ""
},
"uba1408i": {
"target_site": "GGCC",
"name": "Uba1408I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1408",
"methylation_site": ""
},
"tru1i": {
"target_site": "T^TAA",
"name": "Tru1I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Krikstulyte, V., Trinkunaite, L., Maneliene, Z., Kiuduliene, L., Petrusyte, M., Butkus, V., Unpublished observations."
],
"prototype": "MseI",
"organism": "Thermus ruber RFL1",
"methylation_site": ""
},
"bse15i": {
"target_site": "C^YCGRG",
"name": "Bse15I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D., Tereschenko, T., Chizikov, V.E., Andreeva, I., (1995) Gene, vol. 157, pp. 321-322."
],
"prototype": "AvaI",
"organism": "Bacillus species 15",
"methylation_site": ""
},
"msp67i": {
"target_site": "CC^NGG",
"name": "Msp67I",
"suppliers": [],
"source": "M. Sargent",
"references": [
"Walker, J.N.B., Dean, P.G., Saunders, J.R., Unpublished observations."
],
"prototype": "ScrFI",
"organism": "Moraxella species MS67",
"methylation_site": ""
},
"bcoki": {
"target_site": "CTCTTC(1/4)",
"name": "BcoKI",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Kovalevskaya, N.P., Zhelenznaya, L.A., Zelinskaya, N.V., Matvienko, N.I., (1994) Mikrobiologiia, vol. 63, pp. 235-238.",
"Svadbina, I.V., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (2005) Biochemistry, vol. 70, pp. 1363-1366.",
"Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P., Zheleznaya, L.A., Matvienko, N.I., (2004) Biokhimiia, vol. 69, pp. 372-379."
],
"prototype": "Ksp632I",
"organism": "Bacillus coagulans",
"methylation_site": "1(4)"
},
"mmu5i": {
"target_site": "GATC",
"name": "Mmu5I",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2004) FEMS Microbiol. Lett., vol. 236, pp. 91-95.",
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2005) Mol. Biol. Rep., vol. 32, pp. 281-284."
],
"prototype": "MboI",
"organism": "Mitsuokella multiacida 46/5",
"methylation_site": "4(5)"
},
"fali": {
"target_site": "(8/13)AAGNNNNNCTT(13/8)",
"name": "FalI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "",
"organism": "Flavobacterium aquatile Ob10",
"methylation_site": ""
},
"bspst5i": {
"target_site": "GCATC(5/9)",
"name": "BspST5I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Matvienko, N.N., Zheleznaya, L.A., Zelinskaya, N.V., Matvienko, N.I., (1997) Biokhimiia, vol. 62, pp. 304-306.",
"Zelinskaya, N.V., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (1995) Biokhimiia, vol. 60, pp. 1999-2010.",
"Zelinskaya, N.V., Matvienko, N.N., Zheleznaya, L.A., Matvienko, N.I., (1996) Biokhimiia, vol. 61, pp. 1006-1014."
],
"prototype": "SfaNI",
"organism": "Bacillus species ST5",
"methylation_site": "3(6)"
},
"fdiii": {
"target_site": "TGC^GCA",
"name": "FdiII",
"suppliers": [],
"source": "PCC 7601",
"references": [
"Streips, U., Golemboski, B., Unpublished observations.",
"van den Hondel, C.A.M.J.J., van Leen, R.W., van Arkel, G.A., Duyvesteyn, M., de Waard, A., (1983) FEMS Microbiol. Lett., vol. 16, pp. 7-12."
],
"prototype": "MstI",
"organism": "Fremyella diplosiphon",
"methylation_site": ""
},
"uba1334i": {
"target_site": "GGATCC",
"name": "Uba1334I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1334",
"methylation_site": ""
},
"banii": {
"target_site": "GRGCY^C",
"name": "BanII",
"suppliers": [
"Takara Bio Inc. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)",
"EURx Ltd. (1/11)"
],
"source": "IAM 1077",
"references": [
"Forrow, S., Lee, M., Souhami, R.L., Hartley, J.A., (1994) ACS Abstracts, vol. 208, pp. 97.",
"Kilz, S., Kroeger, M., Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Lunnen, K.D., Barsomian, J.M., Camp, R.R., Card, C.O., Chen, S.-Z., Croft, R., Looney, M.C., Meda, M.M., Moran, L.S., Nwankwo, D.O., Slatko, B.E., Van Cott, E.M., Wilson, G.G., (1988) Gene, vol. 74, pp. 25-32.",
"Lunnen, K.D., Kilz, S., Kroeger, M., Wilson, G.G., Unpublished observations.",
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Nucleic Acids Res., vol. 10, pp. 5747-5752.",
"Wilson, G.G., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Bacillus aneurinolyticus",
"methylation_site": "4(5)"
},
"srl56di": {
"target_site": "C^TRYAG",
"name": "Srl56DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Molnarova, V., Javorsky, P., (2002) Biologia (Bratisl), vol. 57, pp. 777-782.",
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "SfeI",
"organism": "Selenomonas ruminantium 56",
"methylation_site": ""
},
"ecidi": {
"target_site": "CCSGG",
"name": "EciDI",
"suppliers": [],
"source": "B. Levin",
"references": [
"Croft, R., Levin, B., Unpublished observations."
],
"prototype": "CauII",
"organism": "Escherichia coli T20",
"methylation_site": ""
},
"uba1376i": {
"target_site": "CCSGG",
"name": "Uba1376I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL1376",
"methylation_site": ""
},
"cdpi": {
"target_site": "GCGGAG(20/18)",
"name": "CdpI",
"suppliers": [],
"source": "NCTC 13129",
"references": [
"Cerdeno-Tarraga, A.M. et al., (2003) Nucleic Acids Res., vol. 31, pp. 6516-6523.",
"Morgan, R.D., Unpublished observations.",
"Usuda, Y., Nishio, Y., Matsui, K., Sugimoto, S., Koseki, K., US Patent Office, 2008."
],
"prototype": "",
"organism": "Corynebacterium diphtheriae",
"methylation_site": "?(6)"
},
"bst6i": {
"target_site": "CTCTTC(1/4)",
"name": "Bst6I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Ksp632I",
"organism": "Bacillus stearothermophilus 6",
"methylation_site": ""
},
"cviki-1": {
"target_site": "RG^CY",
"name": "CviKI-1",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 1772",
"references": [
"Xu, S.-Y., Unpublished observations."
],
"prototype": "CviJI",
"organism": "Chlorella strain NC64A (CA-1A)",
"methylation_site": ""
},
"ngogv": {
"target_site": "GGNNCC",
"name": "NgoGV",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Gregoire, S.T., (1986) M.Sc. Thesis. Univ. Maryland., vol. 0, pp. 1-138.",
"Stein, D.C., Gunn, J.S., Radlinska, M., Piekarowicz, A., (1995) Gene, vol. 157, pp. 19-22."
],
"prototype": "NlaIV",
"organism": "Neisseria gonorrhoeae WR220",
"methylation_site": ""
},
"ftnui": {
"target_site": "?",
"name": "FtnUI",
"suppliers": [],
"source": "F. Nano",
"references": [
"Gallagher, L.A., McKevitt, M., Ramage, E.R., Manoil, C., (2008) J. Bacteriol., vol. 190, pp. 7830-7837."
],
"prototype": "",
"organism": "Francisella tularensis subsp. novicida U112",
"methylation_site": "?(6)"
},
"bsp128i": {
"target_site": "GGWCC",
"name": "Bsp128I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus species RFL128",
"methylation_site": ""
},
"ngogi": {
"target_site": "RGCGCY",
"name": "NgoGI",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Gunn, J.S., Stein, D.C., (1996) Mol. Gen. Genet., vol. 251, pp. 509-517."
],
"prototype": "HaeII",
"organism": "Neisseria gonorrhoeae WR220",
"methylation_site": ""
},
"sfaai": {
"target_site": "GCGAT^CGC",
"name": "SfaAI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G309",
"references": [
"Lauciuniene, N., Trinkunaite, L., Capskaja, L., Vitkute, J., Janulaitis, A., Unpublished observations.",
"Zakareviciene, L., Lubys, A., Unpublished observations."
],
"prototype": "SgfI",
"organism": "Sphingomonas faenia RFL1",
"methylation_site": ""
},
"saudi": {
"target_site": "GATC",
"name": "SauDI",
"suppliers": [],
"source": "NCTC 8358",
"references": [
"Stobberingh, E.E., Schiphof, R., Sussenbach, J.S., (1977) J. Bacteriol., vol. 131, pp. 645-649."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus 55",
"methylation_site": ""
},
"uba1329i": {
"target_site": "GRGCYC",
"name": "Uba1329I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1329",
"methylation_site": ""
},
"ecohi": {
"target_site": "^CCSGG",
"name": "EcoHI",
"suppliers": [],
"source": "L.I. Glatman",
"references": [
"Glatman, L.I., Terekhov, A.A., Kalnin, K.V., Bolotin, A.P., Rebentish, B.A., (1990) Mol. Gen. Mikrobiol. Virusol., vol. 3, pp. 32.",
"Kravetz, A.N., Zakharova, M.V., Beletskaya, I.V., Sineva, E.V., Denjmuchametov, M.M., Petrov, S.I., Glatman, L.I., Solonin, A.S., (1993) Gene, vol. 129, pp. 153-154."
],
"prototype": "CauII",
"organism": "Escherichia coli H1",
"methylation_site": "2(5)"
},
"ecci": {
"target_site": "CCGCGG",
"name": "EccI",
"suppliers": [],
"source": "DSM 30060",
"references": [
"Mayer, H., Klaar, J., Unpublished observations.",
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "SacII",
"organism": "Enterobacter cloacae",
"methylation_site": ""
},
"pae26i": {
"target_site": "CTGCAG",
"name": "Pae26I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Pseudomonas aeruginosa RFL26",
"methylation_site": ""
},
"sani": {
"target_site": "?",
"name": "SanI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Matsui, M., Mise, K., Yoshida, Y., Ishidate, M., (1986) Eisei Shikenjo Hokoku, vol. 104, pp. 92-96."
],
"prototype": "",
"organism": "Salmonella anatum",
"methylation_site": ""
},
"mae843i": {
"target_site": "?",
"name": "Mae843I",
"suppliers": [],
"source": "NIES 843",
"references": [
"Dwinell, E.A., Morgan, R.D., Unpublished observations."
],
"prototype": "",
"organism": "Microcystis aeruginosa",
"methylation_site": "?(6)"
},
"hcui": {
"target_site": "?",
"name": "HcuI",
"suppliers": [],
"source": "D. Oesterhelt",
"references": [
"Schinzel, R., Unpublished observations."
],
"prototype": "",
"organism": "Halobacterium cutirubrum",
"methylation_site": ""
},
"uba1448i": {
"target_site": "CTCGAG",
"name": "Uba1448I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1448",
"methylation_site": ""
},
"sgr20i": {
"target_site": "CCWGG",
"name": "Sgr20I",
"suppliers": [],
"source": "A.V. Orekhov",
"references": [
"Orekhov, A.V., Rebentish, B.A., Debabov, V.G., (1982) Dokl. Akad. Nauk., vol. 263, pp. 217-220."
],
"prototype": "EcoRII",
"organism": "Streptomyces griseus Kr. 20",
"methylation_site": ""
},
"syni": {
"target_site": "GGWCC",
"name": "SynI",
"suppliers": [],
"source": "PCC 6308",
"references": [
"Foerg, E., Saporito, L., Huang, S., Yang, J., Allen, M.M., (1990) FEMS Microbiol. Lett., vol. 69, pp. 105-108."
],
"prototype": "AvaII",
"organism": "Synechocystis species",
"methylation_site": ""
},
"uba1118i": {
"target_site": "CCWGG",
"name": "Uba1118I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL1118",
"methylation_site": ""
},
"eco121i": {
"target_site": "CCSGG",
"name": "Eco121I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "CauII",
"organism": "Escherichia coli RFL121",
"methylation_site": ""
},
"pasi": {
"target_site": "CC^CWGGG",
"name": "PasI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Maneliene, Z., Zakareviciene, L., Unpublished observations.",
"Nekrasiene, D., Vitkute, J., Capskaja, L., Trinkunaite, L., Kiuduliene, L., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Pseudomonas anguilliseptica RFL1",
"methylation_site": ""
},
"nspgi": {
"target_site": "GGWCC",
"name": "NspGI",
"suppliers": [],
"source": "C.P. Wolk",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Nostoc species 23-9B",
"methylation_site": ""
},
"rma486i": {
"target_site": "CTAG",
"name": "Rma486I",
"suppliers": [],
"source": "ITI 486",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"uba1413i": {
"target_site": "GGWCC",
"name": "Uba1413I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL1413",
"methylation_site": ""
},
"uba1147i": {
"target_site": "GGCC",
"name": "Uba1147I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1147",
"methylation_site": ""
},
"r1.ssu11318i": {
"target_site": "GATC",
"name": "R1.Ssu11318I",
"suppliers": [],
"source": "T. Sekizaki",
"references": [
"Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y., (2001) J. Bacteriol., vol. 183, pp. 5436-5440."
],
"prototype": "MboI",
"organism": "Streptococcus suis NIAH11318",
"methylation_site": ""
},
"bstqi": {
"target_site": "GGATCC",
"name": "BstQI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Kong, H., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Bacillus stearothermophilus Q407",
"methylation_site": ""
},
"bsmgi": {
"target_site": "TGTACA",
"name": "BsmGI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "Bsp1407I",
"organism": "Bacillus stearothermophilus G170",
"methylation_site": ""
},
"nani": {
"target_site": "GATATC",
"name": "NanI",
"suppliers": [],
"source": "ATCC 19573",
"references": [
"Dingman, C., Schildkraut, I., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Neisseria animalis",
"methylation_site": ""
},
"hpyf31i": {
"target_site": "GTAC",
"name": "HpyF31I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL31",
"methylation_site": ""
},
"espii": {
"target_site": "?",
"name": "EspII",
"suppliers": [],
"source": "PCC 6906",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "",
"organism": "Eucapsis species",
"methylation_site": ""
},
"uba1150i": {
"target_site": "GGCC",
"name": "Uba1150I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1150",
"methylation_site": ""
},
"bsp2i": {
"target_site": "ATCGAT",
"name": "Bsp2I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species RFL2",
"methylation_site": ""
},
"eco169i": {
"target_site": "GGYRCC",
"name": "Eco169I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Escherichia coli RFL169",
"methylation_site": ""
},
"bseli": {
"target_site": "CCNNNNN^NNGG",
"name": "BseLI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Kazlauskiene, R., Maneliene, Z., Capskaja, L., Vonseviciene, E., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BsiYI",
"organism": "Bacillus stearothermophilus LK3-551",
"methylation_site": ""
},
"cpaai": {
"target_site": "CGCG",
"name": "CpaAI",
"suppliers": [],
"source": "ATCC 6013",
"references": [
"Richards, D.F., Linnett, P.E., Oultram, J.D., Young, M., (1988) J. Gen. Microbiol., vol. 134, pp. 3151-3157."
],
"prototype": "FnuDII",
"organism": "Clostridium pasteurianum",
"methylation_site": ""
},
"bsrmi": {
"target_site": "GATC",
"name": "BsrMI",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, W., Pan, X., Chen, Z., Unpublished observations."
],
"prototype": "MboI",
"organism": "Bacillus stearothermophilus CPW13",
"methylation_site": ""
},
"eco47i": {
"target_site": "G^GWCC",
"name": "Eco47I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Butkus, V.V., Petrusyte, M.P., Janulaitis, A., (1985) Bioorg. Khim., vol. 11, pp. 987-988.",
"Duhring, U., Enke, H., Grundel, M., Lockau, W., Smith, C.R., Woods, P., Ziegler, K., Oesterhelt, C., Coleman, J.R., Kramer, D., International Patent Office, 2009.",
"Janulaitis, A., Petrusyte, M., Butkus, V., (1983) FEBS Lett., vol. 161, pp. 213-216.",
"Lubys, A., Bagdanaviciute, J., Zakareviciene, L., Unpublished observations.",
"Lubys, A., Pagarauskaite, K., Unpublished observations.",
"Song, J., Unpublished observations.",
"Zhong, S., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Escherichia coli RFL47",
"methylation_site": ""
},
"hpyf40i": {
"target_site": "ACNGT",
"name": "HpyF40I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL40",
"methylation_site": ""
},
"mviii": {
"target_site": "?",
"name": "MviII",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Morris, D.W., Parish, J.H., (1976) Arch. Microbiol., vol. 108, pp. 227-230."
],
"prototype": "",
"organism": "Myxococcus virescens V-2",
"methylation_site": ""
},
"asuiii": {
"target_site": "GR^CGYC",
"name": "AsuIII",
"suppliers": [],
"source": "CCAP 1403/4b",
"references": [
"de Waard, A., Duyvesteyn, M., (1980) Arch. Microbiol., vol. 128, pp. 242-247."
],
"prototype": "AcyI",
"organism": "Anabaena subcylindrica",
"methylation_site": ""
},
"sgri": {
"target_site": "?",
"name": "SgrI",
"suppliers": [],
"source": "ATCC 23345",
"references": [
"Arrand, J.R., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Streptomyces griseus",
"methylation_site": ""
},
"cpoi": {
"target_site": "CG^GWCCG",
"name": "CpoI",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)"
],
"source": "ATCC 33010",
"references": [
"Kotani, H., Sagawa, H., Nakajima, K., Unpublished observations.",
"Polisson, C., Unpublished observations."
],
"prototype": "RsrII",
"organism": "Caseobacter polymorphus",
"methylation_site": ""
},
"uba1383i": {
"target_site": "GGATCC",
"name": "Uba1383I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1383",
"methylation_site": ""
},
"bsp117i": {
"target_site": "GRGCYC",
"name": "Bsp117I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Bacillus species RFL117",
"methylation_site": ""
},
"bamfi": {
"target_site": "GGATCC",
"name": "BamFI",
"suppliers": [],
"source": "ATCC 23350",
"references": [
"Borriss, R., Chen, X., Rueckert, C., Blom, J., Becker, A., Baumgarth, B., Fan, B., Pukall, R., Schumann, P., Sproer, C., Junge, H., Vater, J., Puhler, A., Klenk, H.P.",
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "BamHI",
"organism": "Bacillus amyloliquefaciens F",
"methylation_site": ""
},
"tfei": {
"target_site": "?",
"name": "TfeI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Morimoto, Y., Sugio, T., Tano, T., Unpublished observations."
],
"prototype": "",
"organism": "Thiobacillus ferrooxidans AP19-3",
"methylation_site": ""
},
"pru2i": {
"target_site": "GG^CC",
"name": "Pru2I",
"suppliers": [],
"source": "G. Avgustin",
"references": [
"Accetto, T., Peterka, M., Avgustin, G., (2005) FEMS Microbiol. Lett., vol. 247, pp. 177-183."
],
"prototype": "HaeIII",
"organism": "Previtella ruminicola 23",
"methylation_site": ""
},
"uba1439i": {
"target_site": "CCGG",
"name": "Uba1439I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HpaII",
"organism": "Unidentified bacterium RFL1439",
"methylation_site": ""
},
"aoci": {
"target_site": "CC^TNAGG",
"name": "AocI",
"suppliers": [],
"source": "CCAP 1403/9",
"references": [
"de Waard, A., Unpublished observations."
],
"prototype": "SauI",
"organism": "Anabaena species",
"methylation_site": ""
},
"uba1129i": {
"target_site": "CGATCG",
"name": "Uba1129I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Unidentified bacterium RFL1129",
"methylation_site": ""
},
"sau98i": {
"target_site": "CTYRAG",
"name": "Sau98I",
"suppliers": [],
"source": "I. Mikula",
"references": [
"Godany, A., Bukovska, G., Farkasovska, J., Brnakova, Z., Dmitriev, A., Tkacikova, L., Ayele, T., Mikula, I., (2004) Folia Microbiol. (Praha), vol. 49, pp. 307-314."
],
"prototype": "SmlI",
"organism": "Staphylococcus aureus 98",
"methylation_site": ""
},
"srl32di": {
"target_site": "CTGCAG",
"name": "Srl32DI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1997) Folia Microbiol. (Praha), vol. 42, pp. 121-124."
],
"prototype": "PstI",
"organism": "Selenomonas ruminantium 32",
"methylation_site": ""
},
"hpyf27ii": {
"target_site": "TCNGA",
"name": "HpyF27II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy188I",
"organism": "Helicobacter pylori RFL27",
"methylation_site": ""
},
"paei": {
"target_site": "GCATG^C",
"name": "PaeI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "N.N. Sokolov",
"references": [
"Rimseliene, R., Vaisvila, R., Janulaitis, A., Unpublished observations.",
"Sokolov, N.N., Fitsner, A.B., Anikeitcheva, N.V., Choroshoutina, Y.B., Samko, O.T., Kolosha, V.O., Fodor, I., Votrin, I.I., (1985) Mol. Biol. Rep., vol. 10, pp. 159-161."
],
"prototype": "SphI",
"organism": "Pseudomonas aeruginosa",
"methylation_site": ""
},
"tsp132i": {
"target_site": "GGCC",
"name": "Tsp132I",
"suppliers": [],
"source": "ITI 132",
"references": [
"Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Thermus species",
"methylation_site": ""
},
"uba1400i": {
"target_site": "GATATC",
"name": "Uba1400I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRV",
"organism": "Unidentified bacterium RFL1400",
"methylation_site": ""
},
"hpyf59ii": {
"target_site": "GTAC",
"name": "HpyF59II",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori RFL59",
"methylation_site": ""
},
"ecl1zii": {
"target_site": "CCWGG",
"name": "Ecl1zII",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae 1z",
"methylation_site": ""
},
"nladi": {
"target_site": "GATC",
"name": "NlaDI",
"suppliers": [],
"source": "D.C. Stein",
"references": [
"Stein, D.C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Neisseria lactamica 5841",
"methylation_site": ""
},
"sinbi": {
"target_site": "GGWCC",
"name": "SinBI",
"suppliers": [],
"source": "Y. Yoshida",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Salmonella infantis YY190",
"methylation_site": ""
},
"hpyf57i": {
"target_site": "GGCC",
"name": "HpyF57I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori RFL57",
"methylation_site": ""
},
"llai": {
"target_site": "?",
"name": "LlaI",
"suppliers": [],
"source": "T.R. Klaenhammer",
"references": [
"Djordjevic, G.M., Klaenhammer, T.R., (1995) Abstr. Gen. Meet. Am. Soc. Microbiol., vol. 95, pp. 527.",
"Hill, C., Miller, L., Klaenhammer, T., (1990) Plasmid, vol. 23, pp. 167-168.",
"Klaenhammer, T.R., Romero, D., Sing, W., Hill, C., (1991) Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci, ed. Dunny, G.M., Cleary, P.P., McKay, L.L. (American Society for Microbiology, Washington), vol. 8, pp. 124-130."
],
"prototype": "",
"organism": "Lactococcus lactis subsp. lactis",
"methylation_site": "?(6)"
},
"rma519i": {
"target_site": "CTAG",
"name": "Rma519I",
"suppliers": [],
"source": "ITI 519",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"spli": {
"target_site": "C^GTACG",
"name": "SplI",
"suppliers": [],
"source": "M. Kawamura",
"references": [
"Kawamura, M., Sakakibara, M., Watanabe, T., Kita, K., Hiraoka, N., Obayashi, A., Takagi, M., Yano, K., (1986) Nucleic Acids Res., vol. 14, pp. 1985-1989."
],
"prototype": "",
"organism": "Spirulina platensis",
"methylation_site": ""
},
"uba1446i": {
"target_site": "CGCG",
"name": "Uba1446I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Unidentified bacterium RFL1446",
"methylation_site": ""
},
"daqi": {
"target_site": "GTGCAC",
"name": "DaqI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "ApaLI",
"organism": "Deleya aquamana",
"methylation_site": ""
},
"uba1161i": {
"target_site": "ATCGAT",
"name": "Uba1161I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1161",
"methylation_site": ""
},
"aquiii": {
"target_site": "GAGGAG(20/18)",
"name": "AquIII",
"suppliers": [],
"source": "PCC 7002",
"references": [
"Morgan, R.D., Dwinell, E.A., Unpublished observations."
],
"prototype": "BseRI",
"organism": "Agmenellum quadruplicatum PR-6",
"methylation_site": ""
},
"pdmi": {
"target_site": "GAANN^NNTTC",
"name": "PdmI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Vitkute, J., Mociskyte, S., Vaitkevicius, D., Maneliene, Z., Norgeliene, D., Kiuduliene, L., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "XmnI",
"organism": "Pseudomonas diminuta Auk 5-324",
"methylation_site": ""
},
"ssp4i": {
"target_site": "CTCGAG",
"name": "Ssp4I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Streptomyces species RFL4",
"methylation_site": ""
},
"asp78i": {
"target_site": "AGGCCT",
"name": "Asp78I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "StuI",
"organism": "Alcaligenes species 78",
"methylation_site": ""
},
"cjep338ii": {
"target_site": "GCATC",
"name": "CjeP338II",
"suppliers": [],
"source": "J.M.B. Vitor",
"references": [
"Vitor, J.M.B., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Campylobacter jejuni P338",
"methylation_site": ""
},
"m.ecot2dam": {
"target_site": "GATC",
"name": "M.EcoT2Dam",
"suppliers": [],
"source": "S. Hattman",
"references": [
"Revel, H.R., Hattman, S.M., (1971) Virology, vol. 45, pp. 484-495.",
"van Ormondt, H., Gorter, J., Havelaar, K.J., de Waard, A., (1975) Nucleic Acids Res., vol. 2, pp. 1391-1400."
],
"prototype": "MboI",
"organism": "Escherichia coli bacteriophage T2",
"methylation_site": "2(6)"
},
"bst2bi": {
"target_site": "CACGAG(-5/-1)",
"name": "Bst2BI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Belichenko, O.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BsiI",
"organism": "Bacillus stearothermophilus 2B",
"methylation_site": ""
},
"bfishi": {
"target_site": "GATC",
"name": "BfiSHI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193."
],
"prototype": "MboI",
"organism": "Butyrivibrio fibrisolvens SH-1",
"methylation_site": ""
},
"bsp1720i": {
"target_site": "GC^TNAGC",
"name": "Bsp1720I",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I., (1991) Nucleic Acids Res., vol. 19, pp. 2504."
],
"prototype": "EspI",
"organism": "Bacillus species 1720",
"methylation_site": ""
},
"uba1187i": {
"target_site": "CCGCGG",
"name": "Uba1187I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SacII",
"organism": "Unidentified bacterium RFL1187",
"methylation_site": ""
},
"pnti": {
"target_site": "CGATCG",
"name": "PntI",
"suppliers": [],
"source": "ATCC 14072",
"references": [
"Mattila, P., Pitkanen, K., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Propionibacterium intermedium",
"methylation_site": ""
},
"uban11ii": {
"target_site": "CCWGG",
"name": "UbaN11II",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Gomez-Rodriguez, J.A., Huete-Perez, J.A., (2008) Encuentro, vol. 15, pp. 70-87."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium M16-02HSJ, Nicaragua",
"methylation_site": ""
},
"tspei": {
"target_site": "^AATT",
"name": "TspEI",
"suppliers": [
"Toyobo Biochemicals (9/09)"
],
"source": "R.A.D. Williams",
"references": [
"Raven, N.D.H., Kelly, C.D., Carter, N.D., Eastlake, P., Brown, C., Williams, R.A.D., (1993) Gene, vol. 131, pp. 83-86."
],
"prototype": "",
"organism": "Thermus species strain 1E",
"methylation_site": ""
},
"pbumzi": {
"target_site": "ATTAAT",
"name": "PbuMZI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Mrazek, J., Piknova, M., Pristas, P., Kopecny, J., (2005) Anaerobe, vol. 11, pp. 280-284."
],
"prototype": "VspI",
"organism": "Pseudobutyrivibrio species MZ 1",
"methylation_site": ""
},
"vpakutai": {
"target_site": "GGNCC",
"name": "VpaKutAI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "AsuI",
"organism": "Vibrio parahaemolyticus TVP1659",
"methylation_site": ""
},
"mgli": {
"target_site": "?",
"name": "MglI",
"suppliers": [],
"source": "J. Davies",
"references": [
"Smith, D.I., Blattner, F.R., Davies, J., (1976) Nucleic Acids Res., vol. 3, pp. 343-353."
],
"prototype": "",
"organism": "Moraxella glueidi LG1",
"methylation_site": ""
},
"hsui": {
"target_site": "A^AGCTT",
"name": "HsuI",
"suppliers": [],
"source": "ATCC 19417",
"references": [
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Haemophilus suis",
"methylation_site": ""
},
"hpy178vii": {
"target_site": "GGCC",
"name": "Hpy178VII",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Xu, Q., Blaser, M., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Helicobacter pylori J178",
"methylation_site": ""
},
"bsp70i": {
"target_site": "CGCG",
"name": "Bsp70I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Bacillus species RFL70",
"methylation_site": ""
},
"bsp1593i": {
"target_site": "GGCC",
"name": "Bsp1593I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus 1593",
"methylation_site": ""
},
"xmaci": {
"target_site": "C^CCGGG",
"name": "XmaCI",
"suppliers": [],
"source": "M.M. Wijdenbosch",
"references": [
"Rahman, H., Affram, C., Unpublished observations."
],
"prototype": "SmaI",
"organism": "Xanthomonas malvacearum strain C",
"methylation_site": ""
},
"ccyi": {
"target_site": "^GATC",
"name": "CcyI",
"suppliers": [],
"source": "C.S. Chen",
"references": [
"Wen, T.N., Tung, P.H., Chen, C.S., (1989) Bot. Bull. Acad. Sinica, vol. 30, pp. 91-98."
],
"prototype": "MboI",
"organism": "Clostridium cylindrosporum",
"methylation_site": ""
},
"smai": {
"target_site": "CCC^GGG",
"name": "SmaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"American Allied Biochemical, Inc. (4/10)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"Bangalore Genei (1/11)",
"Vivantis Technologies (11/10)",
"EURx Ltd. (1/11)",
"CinnaGen Inc. (1/11)"
],
"source": "ATCC 49779",
"references": [
"Butkus, V., Petrauskiene, L., Maneliene, Z., Klimasauskas, S., Laucys, V., Janulaitis, A., (1987) Nucleic Acids Res., vol. 15, pp. 7091-7102.",
"Endow, S.A., Roberts, R.J., (1977) J. Mol. Biol., vol. 112, pp. 521-529.",
"Greene, R., Mulder, C., Unpublished observations.",
"Klimasauskas, S., Steponaviciene, D., Maneliene, Z., Petrusyte, M., Butkus, V., Janulaitis, A., (1990) Nucleic Acids Res., vol. 18, pp. 6607-6609."
],
"prototype": "",
"organism": "Serratia marcescens Sb",
"methylation_site": "2(4)"
},
"maeii": {
"target_site": "A^CGT",
"name": "MaeII",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "K.O. Stetter",
"references": [
"Schmid, K., Thomm, M., Laminet, A., Laue, F.G., Kessler, C., Stetter, K.O., Schmitt, R., (1984) Nucleic Acids Res., vol. 12, pp. 2619-2628.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "",
"organism": "Methanococcus aeolicus PL-15/H",
"methylation_site": ""
},
"mabi": {
"target_site": "A^CCWGGT",
"name": "MabI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Nadeev, A.N., Tsaplin, M.M., Abdurashitov, M.A., Dedkov, V.S., Kashirina, J.G., Popichenko, D.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SexAI",
"organism": "Microbacterium arborescens SE",
"methylation_site": ""
},
"accbsi": {
"target_site": "CCGCTC(-3/-3)",
"name": "AccBSI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Myakisheva, T.V., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.K., (1997) Prikl. Biokhim. Mikrobiol., vol. 33, pp. 556-558."
],
"prototype": "BsrBI",
"organism": "Acinetobacter calcoaceticus BS",
"methylation_site": ""
},
"pfli": {
"target_site": "?",
"name": "PflI",
"suppliers": [],
"source": "IFO 3507",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "",
"organism": "Pseudomonas fluorescens",
"methylation_site": ""
},
"ofoi": {
"target_site": "C^YCGRG",
"name": "OfoI",
"suppliers": [],
"source": "V. Nagaraja",
"references": [
"Saravanan, M., Elango, K., Chandrashekaran, S., Anand, N., Nagaraja, V., (2003) Curr. Sci., vol. 85, pp. 188-190."
],
"prototype": "AvaI",
"organism": "Oscillatoria foreaui A-1340",
"methylation_site": ""
},
"esphk7i": {
"target_site": "CCWGG",
"name": "EspHK7I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Enterobacter species N7",
"methylation_site": ""
},
"bstci": {
"target_site": "GGCC",
"name": "BstCI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus stearothermophilus C1",
"methylation_site": ""
},
"asp37i": {
"target_site": "ATCGAT",
"name": "Asp37I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "ClaI",
"organism": "Anabaena species 37",
"methylation_site": ""
},
"uba1239i": {
"target_site": "AGGCCT",
"name": "Uba1239I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1239",
"methylation_site": ""
},
"bfi2411i": {
"target_site": "?",
"name": "Bfi2411I",
"suppliers": [],
"source": "A.R. Reddy",
"references": [
"Jutur, P.P., Hoti, S.L., Reddy, A.R., (2004) Mol. Biol. Rep., vol. 31, pp. 139-142."
],
"prototype": "",
"organism": "Bacillus firmus 2411",
"methylation_site": ""
},
"sdiai": {
"target_site": "CTCGAG",
"name": "SdiAI",
"suppliers": [],
"source": "M. Bae",
"references": [
"Bae, M., Suh, W.-N., Song, E.-S., Lee, K.-J., (1994) Korean J. Appl. Microbiol. Biotechnol., vol. 22, pp. 126-133."
],
"prototype": "XhoI",
"organism": "Streptomyces diastatochromogenes",
"methylation_site": ""
},
"rhei": {
"target_site": "GTCGAC",
"name": "RheI",
"suppliers": [],
"source": "ATCC 21664",
"references": [
"Hurlin, P., Schildkraut, I., Unpublished observations."
],
"prototype": "SalI",
"organism": "Rhodococcus species",
"methylation_site": ""
},
"zrai": {
"target_site": "GAC^GTC",
"name": "ZraI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "NEB 1785",
"references": [
"Dedkov, V.S., Sinichkina, S.A., Popichenko, D.V., Degtyarev, S.Kh., (2001) Biotekhnologiya, vol. 6, pp. 3-7.",
"Wei, H., Unpublished observations.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "AatII",
"organism": "Zoogloea ramigera 11",
"methylation_site": ""
},
"psci": {
"target_site": "A^CATGT",
"name": "PscI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "Fermentas G291",
"references": [
"Lauciuniene, N., Vitkute, J., Maneliene, Z., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "BspLU11I",
"organism": "Paracoccus species RFL1",
"methylation_site": ""
},
"ngodiii": {
"target_site": "CCGCGG",
"name": "NgoDIII",
"suppliers": [],
"source": "J.K. Davies",
"references": [
"Duff, M.K., Davies, J.K., (1988) Gonococci and Meningococci, ed. Poolman, J.T., Zanen, H.C., Meyer, T.F., Heckels, J.E., Makela, P.R.H. (Kluwer Academic Publishers, Dordrecht, The Netherlands), pp. 251-256."
],
"prototype": "SacII",
"organism": "Neisseria gonorrhoeae JKD211",
"methylation_site": ""
},
"rma500i": {
"target_site": "CTAG",
"name": "Rma500I",
"suppliers": [],
"source": "ITI 500",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"sbo13i": {
"target_site": "TCG^CGA",
"name": "Sbo13I",
"suppliers": [],
"source": "NCTC 9361",
"references": [
"Benner, J.S., Unpublished observations.",
"Mise, K., Nakajima, K., Terakado, N., Ishidate, M., (1986) Gene, vol. 44, pp. 165-169.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Zhu, Z., Pedamallu, C.S., Fomenkov, A., Benner, J., Xu, S.Y., (2010) BMC Res. Notes, vol. 3, pp. 139."
],
"prototype": "NruI",
"organism": "Shigella boydii 13",
"methylation_site": ""
},
"nrui": {
"target_site": "TCG^CGA",
"name": "NruI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"SibEnzyme Ltd. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Bangalore Genei (1/11)",
"EURx Ltd. (1/11)"
],
"source": "ATCC 15906",
"references": [
"Comb, D.G., Schildkraut, I., Greenough, L., Unpublished observations.",
"Forrow, S., Lee, M., Souhami, R.L., Hartley, J.A., (1994) ACS Abstracts, vol. 208, pp. 97.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Zhu, Z., Unpublished observations.",
"Zhu, Z., Pedamallu, C.S., Fomenkov, A., Benner, J., Xu, S.-Y., Unpublished observations."
],
"prototype": "",
"organism": "Nocardia rubra",
"methylation_site": ""
},
"uba82i": {
"target_site": "CCWGG",
"name": "Uba82I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL82",
"methylation_site": ""
},
"uba1186i": {
"target_site": "CTGCAG",
"name": "Uba1186I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1186",
"methylation_site": ""
},
"hin8ii": {
"target_site": "CATG",
"name": "Hin8II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lazareviciute, L., Maneliene, Z., Padegimiene, A., Kiuduliene, L., Laucys, V., Bitinaite, J., Gruber, I.M., Polyachenko, V.M., Butkus, V., Janulaitus, A., (1990) Bioorg. Khim., vol. 16, pp. 889-897."
],
"prototype": "NlaIII",
"organism": "Haemophilus influenzae RFL8",
"methylation_site": ""
},
"bshti": {
"target_site": "A^CCGGT",
"name": "BshTI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Rudokas, K., Petrusyte, M., Maneliene, Z., Trinkunaite, L., Kiuduliene, E., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "AgeI",
"organism": "Bacillus sphaericus Jo22-024",
"methylation_site": ""
},
"rrhi": {
"target_site": "GTCGAC",
"name": "RrhI",
"suppliers": [],
"source": "ATCC 14349",
"references": [
"Qiang, B.-Q., Schildkraut, I., Unpublished observations."
],
"prototype": "SalI",
"organism": "Rhodococcus rhodochrous",
"methylation_site": ""
},
"itai": {
"target_site": "GC^NGC",
"name": "ItaI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Stadtler, P., von Strandmann, R.P., Walter, T., Frey, B., Auer, H., Hengstenberg, W., Schmitz, G., (1993) Gene, vol. 137, pp. 347-348."
],
"prototype": "Fnu4HI",
"organism": "Ilyobacter tartaricus",
"methylation_site": ""
},
"hpyf36iv": {
"target_site": "GDGCHC",
"name": "HpyF36IV",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "SduI",
"organism": "Helicobacter pylori RFL36",
"methylation_site": ""
},
"bce83i": {
"target_site": "CTTGAG(16/14)",
"name": "Bce83I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Matvienko, N.N., Kramarov, V.M., Ivanov, L.Y., Matvienko, N.I., (1992) Nucleic Acids Res., vol. 20, pp. 1803.",
"Matvienko, N.N., Kramarov, V.M., Zheleznaya, L.A., Matvienko, N.I., (1993) Biokhimiia, vol. 58, pp. 1845-1860."
],
"prototype": "",
"organism": "Bacillus cereus 83",
"methylation_site": ""
},
"bsp126i": {
"target_site": "ATCGAT",
"name": "Bsp126I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus species RFL126",
"methylation_site": ""
},
"cviji": {
"target_site": "RG^CY",
"name": "CviJI",
"suppliers": [
"Molecular Biology Resources - CHIMERx (1/11)",
"EURx Ltd. (1/11)"
],
"source": "J.L. Van Etten",
"references": [
"Shields, S.L., Burbank, D.E., Grabherr, R., Van Etten, J.L., (1990) Virology, vol. 176, pp. 16-24.",
"Swaminathan, N., Mead, D.A., McMaster, K., George, D., Van Etten, J.L., Skowron, P.M., (1996) Nucleic Acids Res., vol. 24, pp. 2463-2469.",
"Xia, Y., Burbank, D.E., Uher, L., Rabussay, D., Van Etten, J.L., (1987) Nucleic Acids Res., vol. 15, pp. 6075-6090."
],
"prototype": "",
"organism": "Chlorella strain NC64A (IL-3A)",
"methylation_site": "3(5)"
},
"bmaai": {
"target_site": "CGATCG",
"name": "BmaAI",
"suppliers": [],
"source": "ATCC 8513",
"references": [
"Hall, D., Unpublished observations."
],
"prototype": "PvuI",
"organism": "Bacillus macerans",
"methylation_site": ""
},
"uba1198i": {
"target_site": "ATCGAT",
"name": "Uba1198I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1198",
"methylation_site": ""
},
"glai": {
"target_site": "GC^GC",
"name": "GlaI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Nayakshina, T.N., Abdurashitov, M.A., Tomilova, Y.E., Mezentseva, N.V., Dedkov, V.S., Mikhnenkova, N.A., Gonchar, D.A., Degtyarev, S.K., (2006) Biotekhnologiya, vol. 0, pp. 26-30."
],
"prototype": "",
"organism": "Glacial ice bacterium GL29",
"methylation_site": ""
},
"hpyf1i": {
"target_site": "GTSAC",
"name": "HpyF1I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp45I",
"organism": "Helicobacter pylori RFL1",
"methylation_site": ""
},
"spapi": {
"target_site": "GACNNNGTC",
"name": "SpaPI",
"suppliers": [],
"source": "D. Borthakur",
"references": [
"Tragut, V., Xiao, J., Bylina, E.J., Borthakur, D., (1995) J. Appl. Phycol., vol. 7, pp. 561-564."
],
"prototype": "Tth111I",
"organism": "Spirulina platensis strain pacifica",
"methylation_site": ""
},
"ecodxxi": {
"target_site": "TCANNNNNNNRTTC",
"name": "EcoDXXI",
"suppliers": [],
"source": "A. Piekarowicz",
"references": [
"Gubler, M., Braguglia, D., Meyer, J., Piekarowicz, A., Bickle, T.A., (1992) EMBO J., vol. 11, pp. 233-240.",
"Piekarowicz, A., Goguen, J.D., (1986) Eur. J. Biochem., vol. 154, pp. 295-298.",
"Piekarowicz, A., Goguen, J.D., Skrzypek, E., (1985) Eur. J. Biochem., vol. 152, pp. 387-393."
],
"prototype": "",
"organism": "Escherichia coli pDXX1",
"methylation_site": "?(6)"
},
"uba1288i": {
"target_site": "GGCC",
"name": "Uba1288I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1288",
"methylation_site": ""
},
"uba1323i": {
"target_site": "GATC",
"name": "Uba1323I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "MboI",
"organism": "Unidentified bacterium RFL1323",
"methylation_site": ""
},
"caci": {
"target_site": "^GATC",
"name": "CacI",
"suppliers": [],
"source": "G. Reysett",
"references": [
"Azeddoug, H., Hubert, J., Reysset, G., (1989) FEMS Microbiol. Lett., vol. 65, pp. 323-326."
],
"prototype": "MboI",
"organism": "Clostridium acetobutylicum N1-4081",
"methylation_site": ""
},
"hpyf13iii": {
"target_site": "ACGT",
"name": "HpyF13III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "MaeII",
"organism": "Helicobacter pylori RFL13",
"methylation_site": ""
},
"bse54i": {
"target_site": "GGNCC",
"name": "Bse54I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "AsuI",
"organism": "Bacillus species 54",
"methylation_site": ""
},
"esp6i": {
"target_site": "GGYRCC",
"name": "Esp6I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Erwinia species RFL6",
"methylation_site": ""
},
"bcefi": {
"target_site": "ACGGC(12/13)",
"name": "BcefI",
"suppliers": [],
"source": "P. Venetianer",
"references": [
"Venetianer, P., Orosz, A., (1988) Nucleic Acids Res., vol. 16, pp. 3053-3060."
],
"prototype": "",
"organism": "Bacillus cereus sub. fluorescens",
"methylation_site": ""
},
"uba1242i": {
"target_site": "GGATCC",
"name": "Uba1242I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1242",
"methylation_site": ""
},
"tsp510i": {
"target_site": "TCGA",
"name": "Tsp510I",
"suppliers": [],
"source": "ITI 70",
"references": [
"Korpela, J., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Thermus species",
"methylation_site": ""
},
"bsogi": {
"target_site": "CCWGG",
"name": "BsoGI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus stearothermophilus Lipari 29",
"methylation_site": ""
},
"taui": {
"target_site": "GCSG^C",
"name": "TauI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Maneliene, Z., Janulaitis, A., (2001) FEMS Microbiol. Lett., vol. 204, pp. 253-257."
],
"prototype": "",
"organism": "Thermus aquaticus Ma2 3-1",
"methylation_site": ""
},
"sacai": {
"target_site": "GCCGGC",
"name": "SacAI",
"suppliers": [],
"source": "ATCC 21353",
"references": [
"Schneider, A., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Streptomyces achromogenes",
"methylation_site": ""
},
"ngodviii": {
"target_site": "GGTGA",
"name": "NgoDVIII",
"suppliers": [],
"source": "J.K. Davies",
"references": [
"Duff, M.K., Davies, J.K., (1988) Gonococci and Meningococci, ed. Poolman, J.T., Zanen, H.C., Meyer, T.F., Heckels, J.E., Makela, P.R.H. (Kluwer Academic Publishers, Dordrecht, The Netherlands), pp. 251-256."
],
"prototype": "HphI",
"organism": "Neisseria gonorrhoeae JKD211",
"methylation_site": ""
},
"bst77i": {
"target_site": "TGATCA",
"name": "Bst77I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Andreeva, I.S., Sizov, A.A., Khomov, V.V., Unpublished observations."
],
"prototype": "BclI",
"organism": "Bacillus stearothermophilus 77",
"methylation_site": ""
},
"gst1588i": {
"target_site": "CYCGRG",
"name": "Gst1588I",
"suppliers": [],
"source": "NEB 1588",
"references": [
"Pan, X.S., Morgan, R., Unpublished observations."
],
"prototype": "AvaI",
"organism": "Geobacillus stearothermophilus",
"methylation_site": ""
},
"xorki": {
"target_site": "CGATCG",
"name": "XorKI",
"suppliers": [],
"source": "B.M. Lee",
"references": [
"Lee, B.M., Park, Y.J., Park, D.S., Kang, H.W., Kim, J.G., Song, E.S., Park, I.C., Yoon, U.H., Hahn, J.H., Koo, B.S., Lee, G.B., Kim, H., Park, H.S., Yoon, K.O., Kim, J.H., Jung, C.H., Koh, N.H., Seo, J.S., Go, S.J., (2005) Nucleic Acids Res., vol. 33, pp. 577-586."
],
"prototype": "PvuI",
"organism": "Xanthomonas oryzae pv. oryzae KACC10331",
"methylation_site": "?(5)"
},
"ppu6i": {
"target_site": "YACGTR",
"name": "Ppu6I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BsaAI",
"organism": "Pseudomonas putida RFL6",
"methylation_site": ""
},
"bsplu11i": {
"target_site": "A^CATGT",
"name": "BspLU11I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Matvienko, N.N., Zeleznaja, L.A., Matvienko, N.I., (1993) Nucleic Acids Res., vol. 21, pp. 1495."
],
"prototype": "",
"organism": "Bacillus species LU11",
"methylation_site": ""
},
"celii": {
"target_site": "GC^TNAGC",
"name": "CelII",
"suppliers": [
"Roche Applied Science (1/11)"
],
"source": "ATCC 27265",
"references": [
"Calleja, F., de Waard, A., Unpublished observations."
],
"prototype": "EspI",
"organism": "Coccochloris elabens 17a",
"methylation_site": ""
},
"tthhb8i": {
"target_site": "T^CGA",
"name": "TthHB8I",
"suppliers": [],
"source": "T. Oshima",
"references": [
"Masui, R., Kurokawa, K., Nakagawa, N., Tokunaga, F., Koyama, Y., Shibata, T., Oshima, T., Yokoyama, S., Yasunaga, T., Kuramitsu, S., Unpublished observations.",
"McClelland, M., (1981) Nucleic Acids Res., vol. 9, pp. 6795-6804.",
"Sato, S., Nakazawa, K., Shinomiya, T., (1980) J. Biochem. (Tokyo), vol. 88, pp. 737-747.",
"Sato, S., Shinomiya, T., (1978) J. Biochem. (Tokyo), vol. 84, pp. 1319-1321.",
"Uchida, Y., Unpublished observations."
],
"prototype": "TaqI",
"organism": "Thermus thermophilus HB8",
"methylation_site": "4(6)"
},
"scai": {
"target_site": "AGT^ACT",
"name": "ScaI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Minotech Biotechnology (1/11)",
"Fermentas International Inc. (1/11)",
"Nippon Gene Co., Ltd. (1/11)",
"Takara Bio Inc. (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)",
"EURx Ltd. (1/11)"
],
"source": "H. Takahashi",
"references": [
"Grandoni, R.P., Schildkraut, I., Unpublished observations.",
"Stickel, S.K., Roberts, R.J., Unpublished observations.",
"Takahashi, H., Kojima, H., Saito, H., (1985) Biochem. J., vol. 231, pp. 229-232.",
"Xu, S.-Y., Xiao, J.-P., Unpublished observations.",
"Xu, S.-Y., Xiao, J.-P., Unpublished observations."
],
"prototype": "",
"organism": "Streptomyces caespitosus",
"methylation_site": "5(4)"
},
"m.cvisi": {
"target_site": "TGCA",
"name": "M.CviSI",
"suppliers": [],
"source": "J.L. Van Etten",
"references": [
"Que, Q., Zhang, Y., Nelson, M., Ropp, S., Burbank, D.E., Van Etten, J.L., (1997) Gene, vol. 190, pp. 237-244."
],
"prototype": "CviRI",
"organism": "Chlorella strain NC64A (SC-1A)",
"methylation_site": "4(6)"
},
"bpai": {
"target_site": "?",
"name": "BpaI",
"suppliers": [],
"source": "A.R. Reddy",
"references": [
"Jutur, P.P., Reddy, A.R., (2004) Biotechnol. Lett., vol. 26, pp. 929-932."
],
"prototype": "",
"organism": "Bacillus pasteurii strain 1761",
"methylation_site": ""
},
"uba1117i": {
"target_site": "TCGCGA",
"name": "Uba1117I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "NruI",
"organism": "Unidentified bacterium RFL1117",
"methylation_site": ""
},
"ppaai": {
"target_site": "TT^CGAA",
"name": "PpaAI",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Waleron, K., Swiatek, W., Biedrzycka, I., Podhajska, A.J., (2001) FEMS Microbiol. Lett., vol. 198, pp. 135-140."
],
"prototype": "AsuII",
"organism": "Phormidium papyraceum",
"methylation_site": ""
},
"bsutui": {
"target_site": "AT^CGAT",
"name": "BsuTUI",
"suppliers": [
"EURx Ltd. (1/11)"
],
"source": "P.M. Skowron",
"references": [
"Skowron, P.M., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Bacillus subtilis TU",
"methylation_site": ""
},
"ncri": {
"target_site": "A^GATCT",
"name": "NcrI",
"suppliers": [],
"source": "Y. Li",
"references": [
"Li, Y., Fu, P., Shi, L.-Y., (1990) Chinese Biochem. J., vol. 6, pp. 309-313."
],
"prototype": "BglII",
"organism": "Nocardia carnea C-212",
"methylation_site": ""
},
"cfr35i": {
"target_site": "CCWGG",
"name": "Cfr35I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL35",
"methylation_site": ""
},
"lsp1109ii": {
"target_site": "GATC",
"name": "Lsp1109II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Grigaite, R., Trinkunaite, L., Maneliene, Z., Bitinaite, J., Kiuduliene, L., Butkus, V., Unpublished observations."
],
"prototype": "MboI",
"organism": "Listeria species RFL1109",
"methylation_site": ""
},
"asp90ii": {
"target_site": "?",
"name": "Asp90II",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Anabaena species 90",
"methylation_site": ""
},
"kox165i": {
"target_site": "CCWGG",
"name": "Kox165I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Klebsiella oxytoca RFL165",
"methylation_site": ""
},
"m.athdrm1": {
"target_site": "?",
"name": "M.AthDRM1",
"suppliers": [],
"source": "A. Graessmann",
"references": [
"Bevan, M., Murphy, G., Ridley, P., Hudson, S., Bancroft, I., Mewes, H.W., Rudd, S., Lemcke, K., Mayer, K.F.X., Unpublished observations.",
"GenBank, D.B., Unpublished observations."
],
"prototype": "",
"organism": "Arabidopsis thaliana",
"methylation_site": "?(5)"
},
"eco246i": {
"target_site": "GGTCTC",
"name": "Eco246I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL246",
"methylation_site": ""
},
"bme74i": {
"target_site": "GGCC",
"name": "Bme74I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "HaeIII",
"organism": "Bacillus megaterium 74",
"methylation_site": ""
},
"uba48i": {
"target_site": "GGWCC",
"name": "Uba48I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Unidentified bacterium RFL48",
"methylation_site": ""
},
"bsp7i": {
"target_site": "CCSGG",
"name": "Bsp7I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "CauII",
"organism": "Bacillus species RFL7",
"methylation_site": ""
},
"tspri": {
"target_site": "CASTGNN^",
"name": "TspRI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "N.D.H. Raven",
"references": [
"Benner, J., Dalton, M.A., Xu, S., Dore, A., Japanese Patent Office, 2005.",
"Raven, N.D.H., Kelly, C.D., Carter, N.D., Smith, K.E., Williams, R.A.D., Unpublished observations.",
"Xu, S.-Y., Unpublished observations.",
"Xu, S.-Y., Dore, A., Dalton, M., Benner, J., US Patent Office, 2003."
],
"prototype": "",
"organism": "Thermus species strain R",
"methylation_site": "1(5)"
},
"msp11i": {
"target_site": "CTGCAG",
"name": "Msp11I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Lebedev, L.R., Andreeva, I.S., Puchkova, L.I., Zernov, Y.P., Serov, G.D., Tereshchenko, T.A., Aphinogenova, G.N., Pustoshilova, N.M., (1998) Biotekhnologiya, vol. 0, pp. 18-27."
],
"prototype": "PstI",
"organism": "Micrococcus species 11",
"methylation_site": ""
},
"cin1467i": {
"target_site": "GATC",
"name": "Cin1467I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "MboI",
"organism": "Clostridium indolis 1467",
"methylation_site": ""
},
"hpycii": {
"target_site": "?",
"name": "HpyCII",
"suppliers": [],
"source": "NCTC 11637",
"references": [
"Ivic, A., Jakeman, K.J., Penn, C.W., Brown, N.L., (1999) FEMS Microbiol. Lett., vol. 179, pp. 175-180."
],
"prototype": "",
"organism": "Helicobacter pylori",
"methylation_site": ""
},
"sdii": {
"target_site": "GGCCNNNN^NGGCC",
"name": "SdiI",
"suppliers": [],
"source": "F. Kimizuka",
"references": [
"Nomura, Y., Ito, H., Sagawa, H., Kotani, H., Kimizuka, F., Nakajima, K., Unpublished observations."
],
"prototype": "SfiI",
"organism": "Streptomyces diastaticus",
"methylation_site": ""
},
"ecor13i": {
"target_site": "?",
"name": "EcoR13I",
"suppliers": [],
"source": "ATCC 35332",
"references": [
"Barcus, V.A., Titheradge, A.J.B., Murray, N.E., (1995) Genetics, vol. 140, pp. 1187-1197."
],
"prototype": "",
"organism": "Escherichia coli ECOR13",
"methylation_site": "?(6)"
},
"srui": {
"target_site": "TTT^AAA",
"name": "SruI",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Vanat, I., Pristas, P., Rybosoval, E., Godany, A., Javorsky, P., (1993) FEMS Microbiol. Lett., vol. 113, pp. 129-132."
],
"prototype": "AhaIII",
"organism": "Selenomonas ruminantium 18D",
"methylation_site": ""
},
"shyti": {
"target_site": "?",
"name": "ShyTI",
"suppliers": [],
"source": "T. Yamaguchi",
"references": [
"Shimotsu, H., Takahashi, H., Saito, H., (1980) Agric. Biol. Chem., vol. 44, pp. 1665-1666."
],
"prototype": "",
"organism": "Streptomyces hygroscopicus",
"methylation_site": ""
},
"bsui": {
"target_site": "GTATCC(6/5)",
"name": "BsuI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Chernukhin, V.A., Kashirina, E.V., Tarasova, G.V., Dedkov, V.S., Mikhnenkova, N.A., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BciVI",
"organism": "Bacillus sphaericus",
"methylation_site": ""
},
"aclwi": {
"target_site": "GGATC(4/5)",
"name": "AclWI",
"suppliers": [
"SibEnzyme Ltd. (1/11)"
],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "BinI",
"organism": "Acinetobacter calcoaceticus W2131",
"methylation_site": ""
},
"bssci": {
"target_site": "GGCC",
"name": "BssCI",
"suppliers": [],
"source": "N. Welker",
"references": [
"Langdale, J.A., Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Bacillus stearothermophilus C11",
"methylation_site": ""
},
"hpyf19iii": {
"target_site": "TCNNGA",
"name": "HpyF19III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Hpy178III",
"organism": "Helicobacter pylori RFL19",
"methylation_site": ""
},
"ssei": {
"target_site": "TGATCA",
"name": "SseI",
"suppliers": [],
"source": "ATCC 29203",
"references": [
"Brand, P., de Waard, A., Unpublished observations."
],
"prototype": "BclI",
"organism": "Synechococcus species",
"methylation_site": ""
},
"sflhk1794i": {
"target_site": "CCWGG",
"name": "SflHK1794I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Lee, K.F., Ling, J.M.-L., Kam, K.-M., Clark, D.R., Shaw, P.-C., (1997) J. Med. Microbiol., vol. 46, pp. 949-952."
],
"prototype": "EcoRII",
"organism": "Shigella flexneri 1794",
"methylation_site": ""
},
"uba1237i": {
"target_site": "CTCGAG",
"name": "Uba1237I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1237",
"methylation_site": ""
},
"uba41i": {
"target_site": "CCSGG",
"name": "Uba41I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Jagelavicius, M., Bitinaite, J., Unpublished observations."
],
"prototype": "CauII",
"organism": "Unidentified bacterium RFL41",
"methylation_site": ""
},
"m.rradnmti": {
"target_site": "?",
"name": "M.RraDnmtI",
"suppliers": [],
"source": "H. Kimura",
"references": [
"Kimura, H., Takeda, T., Tanaka, S., Ogawa, T., Shiota, K., (1998) Biochem. Biophys. Res. Commun., vol. 253, pp. 495-501."
],
"prototype": "",
"organism": "Rattus rattus",
"methylation_site": "?(5)"
},
"msiii": {
"target_site": "?",
"name": "MsiII",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Mayer, H., Schutte, H., Unpublished observations."
],
"prototype": "",
"organism": "Myxococcus stipitatus Mxs2",
"methylation_site": ""
},
"psc126i": {
"target_site": "?",
"name": "Psc126I",
"suppliers": [],
"source": "K. Sivonen",
"references": [
"Lyra, C., Halme, T., Torsti, A.-M., Tenkanen, T., Sivonen, K., (2000) J. Appl. Microbiol., vol. 89, pp. 979-991."
],
"prototype": "",
"organism": "Planktothrix species NIVA-CYA126",
"methylation_site": ""
},
"bth211i": {
"target_site": "GATC",
"name": "Bth211I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. tolworthi",
"methylation_site": ""
},
"uba13i": {
"target_site": "CCWGG",
"name": "Uba13I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Unidentified bacterium RFL13",
"methylation_site": ""
},
"ssp27144i": {
"target_site": "AT^CGAT",
"name": "Ssp27144I",
"suppliers": [],
"source": "ATCC 27144",
"references": [
"Obayashi, A., Japanese Patent Office, 1985."
],
"prototype": "ClaI",
"organism": "Synechococcus species",
"methylation_site": ""
},
"uba1217i": {
"target_site": "AGGCCT",
"name": "Uba1217I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StuI",
"organism": "Unidentified bacterium RFL1217",
"methylation_site": ""
},
"acc65i": {
"target_site": "G^GTACC",
"name": "Acc65I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)",
"Promega Corporation (7/10)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Prichodko, G.G., Rechnukova, N.I., Repin, V.E., Degtyarev, S.K., (1991) Sib. Biol. J., vol. 1, pp. 59-60."
],
"prototype": "KpnI",
"organism": "Acinetobacter calcoaceticus 65",
"methylation_site": ""
},
"ssibi": {
"target_site": "^GATC",
"name": "SsiBI",
"suppliers": [],
"source": "N.N. Sokolov",
"references": [
"Sokolov, N.N., (1995) Vestn. Akad. Med. Nauk SSSR, vol. 2, pp. 47-51."
],
"prototype": "MboI",
"organism": "Staphylococcus species B",
"methylation_site": ""
},
"bvei": {
"target_site": "ACCTGC(4/8)",
"name": "BveI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "V. Butkus",
"references": [
"Mociskyte, S., Vaitkevicius, D., Maneliene, Z., Trinkunaite, L., Kiuduliene, L., Petrusyte, M., Markauskas, A., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BspMI",
"organism": "Brevundimonas vesicularis RFL1",
"methylation_site": ""
},
"pauaii": {
"target_site": "TTT^AAA",
"name": "PauAII",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Waleron, K., Swiatek, W., Biedrzycka, I., Podhajska, A.J., (2001) FEMS Microbiol. Lett., vol. 198, pp. 135-140."
],
"prototype": "AhaIII",
"organism": "Phormidium autumnale",
"methylation_site": ""
},
"uba1208i": {
"target_site": "GGCC",
"name": "Uba1208I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Unidentified bacterium RFL1208",
"methylation_site": ""
},
"m.rho11si": {
"target_site": "GGCC,GCNGC",
"name": "M.Rho11sI",
"suppliers": [],
"source": "T.A. Trautner",
"references": [
"Behrens, B., Noyer-Weidner, M., Pawlek, B., Lauster, R., Balganesh, T.S., Trautner, T.A., (1987) EMBO J., vol. 6, pp. 1137-1142.",
"Gunthert, U., Trautner, T.A., (1984) Curr. Top. Microbiol. Immunol., vol. 108, pp. 11-22.",
"Noyer-Weidner, M., Jentsch, S., Kupsch, J., Bergbauer, M., Trautner, T.A., (1985) Gene, vol. 35, pp. 143-150.",
"Noyer-Weidner, M., Jentsch, S., Pawlek, B., Gunthert, U., Trautner, T.A., (1983) J. Virol., vol. 46, pp. 446-453."
],
"prototype": "HaeIII",
"organism": "Bacillus subtilis phage Rho11",
"methylation_site": "3(5), 2(5)"
},
"eco231i": {
"target_site": "AAGCTT",
"name": "Eco231I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Kaczorowski, T., Unpublished observations.",
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "HindIII",
"organism": "Escherichia coli RFL231",
"methylation_site": ""
},
"bsgi": {
"target_site": "GTGCAG(16/14)",
"name": "BsgI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 581",
"references": [
"Higgins, L.S., Kong, H., Unpublished observations.",
"Kong, H., Sears, L., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Bacillus sphaericus GC subgroup",
"methylation_site": "?(6)"
},
"bspr7i": {
"target_site": "CC^TNAGG",
"name": "BspR7I",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Vasiljeva, L.Yu., Zheleznaya, L.A., Matvienko, N.I., (1996) Biokhimiia, vol. 61, pp. 2147-2157."
],
"prototype": "SauI",
"organism": "Bacillus species R7",
"methylation_site": ""
},
"seci": {
"target_site": "C^CNNGG",
"name": "SecI",
"suppliers": [],
"source": "ATCC 27170",
"references": [
"Calleja, F., Tandeau de Marsac, N., Coursin, T., van Ormondt, H., de Waard, A., (1985) Nucleic Acids Res., vol. 13, pp. 6745-6751."
],
"prototype": "",
"organism": "Synechocystis species 6701",
"methylation_site": ""
},
"psp03i": {
"target_site": "GGWC^C",
"name": "Psp03I",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Piechula, S., Waleron, K., Swiatek, W., Biedrzycka, I., Podhajska, A.J., (2001) FEMS Microbiol. Lett., vol. 198, pp. 135-140."
],
"prototype": "AvaII",
"organism": "Phormidium species",
"methylation_site": ""
},
"caui": {
"target_site": "G^GWCC",
"name": "CauI",
"suppliers": [],
"source": "A. Bingham",
"references": [
"Bennett, S.P., (1987) Ph.D. Thesis, University of Bristol, UK, pp. 1-196.",
"Bingham, A.H.A., Darbyshire, J., (1982) Gene, vol. 18, pp. 87-91.",
"Molemans, F., van Emmelo, J., Fiers, W., (1982) Gene, vol. 18, pp. 93-96."
],
"prototype": "AvaII",
"organism": "Chloroflexus aurantiacus",
"methylation_site": ""
},
"psp61i": {
"target_site": "GCCGGC",
"name": "Psp61I",
"suppliers": [],
"source": "M. Sargent",
"references": [
"Walker, J.N.B., Dean, P.G., Saunders, J.R., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Pseudomonas species MS61",
"methylation_site": ""
},
"tspidsi": {
"target_site": "ACGT",
"name": "TspIDSI",
"suppliers": [],
"source": "R.A.D. Williams",
"references": [
"Welch, S.G., Williams, R.A.D., (1996) Nucleic Acids Res., vol. 24, pp. 1799-1801."
],
"prototype": "MaeII",
"organism": "Thermus species",
"methylation_site": ""
},
"mlsi": {
"target_site": "TGG^CCA",
"name": "MlsI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Vitkute, J., Savelskiene, A., Maneliene, Z., Trinkunaite, L., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "BalI",
"organism": "Micrococcus luteus Ng 16-122",
"methylation_site": ""
},
"uba1263i": {
"target_site": "GRGCYC",
"name": "Uba1263I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Unidentified bacterium RFL1263",
"methylation_site": ""
},
"rshi": {
"target_site": "CGAT^CG",
"name": "RshI",
"suppliers": [],
"source": "S. Kaplan",
"references": [
"Copeland, A., Lucas, S., Lapidus, A., Barry, K., Detter, J.C., Glavina, T., Hammon, N., Israni, S., Pitluck, S., Richardson, P., Mackenzie, C., Choudhary, M., Larimer, F., Hauser, L.J., Land, M., Donohue, T.J., Kaplan, S., Unpublished observations.",
"Lynn, S.P., Cohen, L.K., Gardner, J.F., Kaplan, S., (1979) J. Bacteriol., vol. 138, pp. 505-509."
],
"prototype": "PvuI",
"organism": "Rhodobacter sphaeroides 2.4.1",
"methylation_site": "?(5)"
},
"apaori": {
"target_site": "CC^WGG",
"name": "ApaORI",
"suppliers": [],
"source": "IFO 13752",
"references": [
"Ishikawa, T., Yamada, Y., (1990) J. Gen. Appl. Microbiol., vol. 36, pp. 127-135."
],
"prototype": "EcoRII",
"organism": "Acetobacter pasteurianus",
"methylation_site": ""
},
"nsplki": {
"target_site": "GG^CC",
"name": "NspLKI",
"suppliers": [],
"source": "N.I. Matvienko",
"references": [
"Zabaznaya, E.V., Zheleznaya, L.A., Svadbina, I.V., Matvienko, N.I., (1999) Biokhimiia, vol. 64, pp. 234-238."
],
"prototype": "HaeIII",
"organism": "Nocardia species LK",
"methylation_site": ""
},
"csui": {
"target_site": "?",
"name": "CsuI",
"suppliers": [],
"source": "ATCC 15264",
"references": [
"Grandoni, R., Unpublished observations."
],
"prototype": "",
"organism": "Caulobacter subvibroides",
"methylation_site": ""
},
"bpsi": {
"target_site": "GGNCC",
"name": "BpsI",
"suppliers": [],
"source": "P. Babu",
"references": [
"Shroff, D., Damodaran, B., Bulla, S., Ramesh, S., Suma, M.A., Kumar, M., Hegde, V., Babu, P., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Bacillus species",
"methylation_site": ""
},
"rma503i": {
"target_site": "CTAG",
"name": "Rma503I",
"suppliers": [],
"source": "ITI 503",
"references": [
"Torsti, A.-M., Hjorleifsdottir, S., Unpublished observations."
],
"prototype": "MaeI",
"organism": "Rhodothermus marinus",
"methylation_site": ""
},
"bcui": {
"target_site": "A^CTAGT",
"name": "BcuI",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Maneliene, Z., Butkus, V., Unpublished observations.",
"Vitkute, J., Zutautiene, D., Valickiene, D., Kiuduliene, E., Petrusyte, M., Butkus, V., Janulaitis, A., Unpublished observations."
],
"prototype": "SpeI",
"organism": "Bacillus coagulans Vs 29-022",
"methylation_site": ""
},
"nliii": {
"target_site": "GGWCC",
"name": "NliII",
"suppliers": [],
"source": "A. de Waard",
"references": [
"Duyvesteyn, M.G.C., Korsuize, J., de Waard, A., Vonshak, A., Wolk, C.P., (1983) Arch. Microbiol., vol. 134, pp. 276-281."
],
"prototype": "AvaII",
"organism": "Nostoc linckia",
"methylation_site": ""
},
"m.cfuiii": {
"target_site": "?",
"name": "M.CfuIII",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Lebenka, A.Yu., Chitavichyus, D.B., (1988) Biokhimiia, vol. 53, pp. 1895-1899."
],
"prototype": "",
"organism": "Caulobacter fusiformis BC-25",
"methylation_site": ""
},
"eco90i": {
"target_site": "YGGCCR",
"name": "Eco90I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "CfrI",
"organism": "Escherichia coli RFL90",
"methylation_site": ""
},
"uba1366ii": {
"target_site": "ATCGAT",
"name": "Uba1366II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "ClaI",
"organism": "Unidentified bacterium RFL1366",
"methylation_site": ""
},
"ahli": {
"target_site": "A^CTAGT",
"name": "AhlI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SpeI",
"organism": "Alteromonas haloplanktis SP",
"methylation_site": ""
},
"bscni": {
"target_site": "CGRYCG",
"name": "BscNI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Lam, P., Clark, D.R., Unpublished observations."
],
"prototype": "McrI",
"organism": "Bacillus species 4515",
"methylation_site": ""
},
"atsi": {
"target_site": "GACN^NNGTC",
"name": "AtsI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "Tth111I",
"organism": "Aureobacterium testaceum 4842",
"methylation_site": ""
},
"lpnii": {
"target_site": "?",
"name": "LpnII",
"suppliers": [],
"source": "A. Brown",
"references": [
"Chen, G.C.C., Brown, A., Lema, M.W., (1986) Can. J. Microbiol., vol. 32, pp. 591-593."
],
"prototype": "",
"organism": "Legionella pneumophila Philadelphia 1",
"methylation_site": ""
},
"asphi": {
"target_site": "GWGCW^C",
"name": "AspHI",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Bolton, B.J., Holtke, H.-J., Schmitz, G.G., Jarsch, M., Kessler, C., (1989) Nucleic Acids Res., vol. 17, pp. 9500."
],
"prototype": "HgiAI",
"organism": "Achromobacter species H",
"methylation_site": ""
},
"bspj74i": {
"target_site": "CTGGAG",
"name": "BspJ74I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "GsuI",
"organism": "Bacillus species RFL74",
"methylation_site": ""
},
"bstpai": {
"target_site": "GACNN^NNGTC",
"name": "BstPAI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"Vivantis Technologies (11/10)"
],
"source": "S.K. Degtyarev",
"references": [
"Tomilova, J.E., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.Kh., Unpublished observations."
],
"prototype": "PshAI",
"organism": "Bacillus stearothermophilus PA",
"methylation_site": ""
},
"pani": {
"target_site": "C^TCGAG",
"name": "PanI",
"suppliers": [],
"source": "IFO 12319",
"references": [
"Sugisaki, H., Maekawa, Y., Kanazawa, S., Takanami, M., (1982) Bull. Inst. Chem. Res. Kyoto Univ., vol. 60, pp. 328-335."
],
"prototype": "XhoI",
"organism": "Pseudomonas alkanolytica",
"methylation_site": ""
},
"ncii": {
"target_site": "CC^SGG",
"name": "NciI",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Promega Corporation (7/10)"
],
"source": "NRCC 31006",
"references": [
"Korch, C., Hagblom, P., (1986) Eur. J. Biochem., vol. 161, pp. 519-524.",
"Lunnen, K.D., Wilson, G.G., Unpublished observations.",
"Meehan, R.R., Ulrich, E., Bird, A.P., (1993) Nucleic Acids Res., vol. 21, pp. 5517-5518.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007.",
"Watson, R., Zuker, M., Martin, S.M., Visentin, L.P., (1980) FEBS Lett., vol. 118, pp. 47-50."
],
"prototype": "CauII",
"organism": "Neisseria cinerea",
"methylation_site": ""
},
"fgoi": {
"target_site": "C^TAG",
"name": "FgoI",
"suppliers": [],
"source": "B.K.C. Patel",
"references": [
"Andrews, K.T., Patel, B.K.C., Clarke, F.M., (1998) Anaerobe, vol. 4, pp. 227-232."
],
"prototype": "MaeI",
"organism": "Fervidobacterium gondwanense AB39T",
"methylation_site": ""
},
"uba1362i": {
"target_site": "GDGCHC",
"name": "Uba1362I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "SduI",
"organism": "Unidentified bacterium RFL1362",
"methylation_site": ""
},
"ecici": {
"target_site": "CCTNAGG",
"name": "EciCI",
"suppliers": [],
"source": "B. Levin",
"references": [
"Croft, R., Levin, B., Unpublished observations."
],
"prototype": "SauI",
"organism": "Escherichia coli T18",
"methylation_site": ""
},
"ddei": {
"target_site": "C^TNAG",
"name": "DdeI",
"suppliers": [
"Invitrogen Corporation (1/11)",
"Roche Applied Science (1/11)",
"New England Biolabs (1/11)",
"Toyobo Biochemicals (9/09)",
"Molecular Biology Resources - CHIMERx (1/11)",
"Promega Corporation (7/10)",
"Sigma Chemical Corporation (1/11)"
],
"source": "NCIB 83120",
"references": [
"Brooks, J.E., Howard, K.A., US Patent Office, 1994.",
"Gelinas, R.E., Roberts, R.J., Unpublished observations.",
"Howard, K.A., Card, C., Benner, J.S., Callahan, H.L., Maunus, R., Silber, K., Wilson, G., Brooks, J.E., (1986) Nucleic Acids Res., vol. 14, pp. 7939-7951.",
"Makula, R.A., Meagher, R.B., (1980) Nucleic Acids Res., vol. 8, pp. 3125-3131.",
"Sznyter, L.A., Slatko, B., Moran, L., O'Donnell, K.H., Brooks, J.E., (1987) Nucleic Acids Res., vol. 15, pp. 8249-8266."
],
"prototype": "",
"organism": "Desulfovibrio desulfuricans Norway strain",
"methylation_site": "1(5)"
},
"uba1302i": {
"target_site": "GGATCC",
"name": "Uba1302I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL1302",
"methylation_site": ""
},
"bpugi": {
"target_site": "RGATCY",
"name": "BpuGI",
"suppliers": [],
"source": "NEB 1359",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Bacillus pumilus 980",
"methylation_site": ""
},
"uba1375i": {
"target_site": "TCCGGA",
"name": "Uba1375I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "BspMII",
"organism": "Unidentified bacterium RFL1375",
"methylation_site": ""
},
"bstz16i": {
"target_site": "GTCGAC",
"name": "BstZ16I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Pan, X.S., Chen, Z., Unpublished observations."
],
"prototype": "SalI",
"organism": "Bacillus stearothermophilus 37Q",
"methylation_site": ""
},
"msp24i": {
"target_site": "GGNCC",
"name": "Msp24I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "AsuI",
"organism": "Moraxella species 24",
"methylation_site": ""
},
"vpak11i": {
"target_site": "GGWCC",
"name": "VpaK11I",
"suppliers": [],
"source": "T. Shimada",
"references": [
"Miyahara, M., Shimada, T., Kotani, H., Mise, K., (1992) Gene, vol. 117, pp. 103-106."
],
"prototype": "AvaII",
"organism": "Vibrio parahaemolyticus 1307",
"methylation_site": ""
},
"sauci": {
"target_site": "GATC",
"name": "SauCI",
"suppliers": [],
"source": "NCTC 8327",
"references": [
"Stobberingh, E.E., Schiphof, R., Sussenbach, J.S., (1977) J. Bacteriol., vol. 131, pp. 645-649."
],
"prototype": "MboI",
"organism": "Staphylococcus aureus 3C",
"methylation_site": ""
},
"aitai": {
"target_site": "RGATCY",
"name": "AitAI",
"suppliers": [],
"source": "ATCC 12639",
"references": [
"Polisson, C., Unpublished observations."
],
"prototype": "XhoII",
"organism": "Aquaspirillum itersonii",
"methylation_site": ""
},
"ecoki": {
"target_site": "AACNNNNNNGTGC",
"name": "EcoKI",
"suppliers": [],
"source": "F.R. Blattner",
"references": [
"Blattner, F.R., Plunkett, G. III, Bloch, C.A., Perna, N.T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J.D., Rode, C.K., Mayhew, G.F., Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J., Mau, B., Shao, Y., (1997) Science, vol. 277, pp. 1453-1462.",
"Gough, J.A., Murray, N.E., (1983) J. Mol. Biol., vol. 166, pp. 1-19.",
"Haberman, A., Heywood, J., Meselson, M., (1972) Proc. Natl. Acad. Sci. U. S. A., vol. 69, pp. 3138-3141.",
"Kan, N.C., Lautenberger, J.A., Edgell, M.H., Hutchison, C.A. III, (1979) J. Mol. Biol., vol. 130, pp. 191-209.",
"Loenen, W.A.M., Daniel, A.S., Braymer, H.D., Murray, N.E., (1987) J. Mol. Biol., vol. 198, pp. 159-170.",
"Meselson, M., Yuan, R., (1968) Nature, vol. 217, pp. 1110-1114.",
"Puzio, P., Ascenzi, R., Mittendorf, V., International Patent Office, 2009.",
"Willcock, D.F., Dryden, D.T.F., Murray, N.E., (1994) EMBO J., vol. 13, pp. 3902-3908."
],
"prototype": "",
"organism": "Escherichia coli K MG1655",
"methylation_site": "2(6),-3(6)"
},
"bspbse18i": {
"target_site": "GGCC",
"name": "BspBSE18I",
"suppliers": [],
"source": "F.G. Priest",
"references": [
"Zahner, V., Priest, F.G., (1997) Lett. Appl. Microbiol., vol. 24, pp. 483-487."
],
"prototype": "HaeIII",
"organism": "Bacillus sphaericus BSE18",
"methylation_site": ""
},
"ecli": {
"target_site": "CAG^CTG",
"name": "EclI",
"suppliers": [],
"source": "H. Hartmann",
"references": [
"Degtyarev, S.K., Unpublished observations.",
"Hartmann, H., Goebel, W., (1977) FEBS Lett., vol. 80, pp. 285-287."
],
"prototype": "PvuII",
"organism": "Enterobacter cloacae",
"methylation_site": ""
},
"saoi": {
"target_site": "GCCGGC",
"name": "SaoI",
"suppliers": [],
"source": "ATCC 25184",
"references": [
"Stote, R., Morgan, R., Unpublished observations."
],
"prototype": "NaeI",
"organism": "Streptomyces albofaciens",
"methylation_site": ""
},
"cjuii": {
"target_site": "CAYNNNNNCTC",
"name": "CjuII",
"suppliers": [],
"source": "Fermentas G311",
"references": [
"Vitkute, J., Lauciuniene, N., Riauba, L., Capskaja, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "",
"organism": "Campylobacter jejuni RFL1",
"methylation_site": ""
},
"accebi": {
"target_site": "G^GATCC",
"name": "AccEBI",
"suppliers": [],
"source": "A. Vivian",
"references": [
"Walker, J.N.B., Vivian, A., Saunders, J.R., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Acinetobacter calcoaceticus EBF 65/65",
"methylation_site": ""
},
"uba1328i": {
"target_site": "CTGCAG",
"name": "Uba1328I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "PstI",
"organism": "Unidentified bacterium RFL1328",
"methylation_site": ""
},
"hpy26i": {
"target_site": "TGCA",
"name": "Hpy26I",
"suppliers": [],
"source": "M.J. Blaser",
"references": [
"Ando, T., Xu, Q., Torres, M., Kusugami, K., Israel, D.A., Blaser, M.J., (2000) Mol. Microbiol., vol. 37, pp. 1052-1065."
],
"prototype": "CviRI",
"organism": "Helicobacter pylori J26",
"methylation_site": ""
},
"drddi": {
"target_site": "CTCGAG",
"name": "DrdDI",
"suppliers": [],
"source": "R.G.E. Murray",
"references": [
"Croft, R., Murray, R.G.E., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Deinococcus radiodurans 1077",
"methylation_site": ""
},
"bbi24i": {
"target_site": "A^CGCGT",
"name": "Bbi24I",
"suppliers": [],
"source": "T. Kanematsu",
"references": [
"Kanematsu, T., Ozawa, O., Murakami, M., Japanese Patent Office, 1991."
],
"prototype": "MluI",
"organism": "Bifidobacterium bifidum S-24",
"methylation_site": ""
},
"mjaiii": {
"target_site": "GATC",
"name": "MjaIII",
"suppliers": [],
"source": "DSM 2661",
"references": [
"Bult, C.J. et al., (1996) Science, vol. 273, pp. 1058-1073.",
"Morgan, R., Posfai, J., Patti, J., Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Methanocaldococcus jannaschii",
"methylation_site": "2(6)"
},
"bth945i": {
"target_site": "GATC",
"name": "Bth945I",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Puchkova, L.I., Kalmykova, G.V., Burtseva, L.I., Repin, V.E., (2002) Prikl. Biokhim. Mikrobiol., vol. 38, pp. 140-144."
],
"prototype": "MboI",
"organism": "Bacillus thuringiensis ssp. tohokuensis",
"methylation_site": ""
},
"uba31i": {
"target_site": "GGATCC",
"name": "Uba31I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Steponaviciene, D., Petrusyte, M., Janulaitis, A., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium RFL31",
"methylation_site": ""
},
"uban3i": {
"target_site": "GGATCC",
"name": "UbaN3I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "BamHI",
"organism": "Unidentified bacterium DCF-05, Nicaragua",
"methylation_site": ""
},
"sstiii": {
"target_site": "?",
"name": "SstIII",
"suppliers": [],
"source": "S. Goff",
"references": [
"Goff, S.P., Rambach, A., (1978) Gene, vol. 3, pp. 347-352."
],
"prototype": "",
"organism": "Streptomyces stanford",
"methylation_site": ""
},
"ecl37i": {
"target_site": "CCGCGG",
"name": "Ecl37I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Adomaviciute, L., Unpublished observations."
],
"prototype": "SacII",
"organism": "Enterobacter cloacae RFL37",
"methylation_site": ""
},
"bsiji": {
"target_site": "?",
"name": "BsiJI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "",
"organism": "Bacillus species",
"methylation_site": ""
},
"bsp519i": {
"target_site": "GRGCY^C",
"name": "Bsp519I",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Degtyarev, S.K., Unpublished observations."
],
"prototype": "HgiJII",
"organism": "Bacillus species 519",
"methylation_site": ""
},
"ksphk12i": {
"target_site": "CCWGG",
"name": "KspHK12I",
"suppliers": [],
"source": "P.C. Shaw",
"references": [
"Chan, Y.C., Kam, K.M., Shaw, P.C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Klebsiella species N12",
"methylation_site": ""
},
"hpy471i": {
"target_site": "GTAC",
"name": "Hpy471I",
"suppliers": [],
"source": "N.R. Salama",
"references": [
"Humbert, O., Salama, N.R., (2008) Nucleic Acids Res., vol. 36, pp. 6893-6906.",
"Salama, N.R., Unpublished observations."
],
"prototype": "RsaI",
"organism": "Helicobacter pylori 47-1",
"methylation_site": ""
},
"mel4oi": {
"target_site": "GATC",
"name": "Mel4OI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Piknova, M., Filova, M., Javorsky, P., Pristas, P., (2004) FEMS Microbiol. Lett., vol. 236, pp. 91-95.",
"Piknova, M., Pristas, P., Javorsky, P., (2004) Folia Microbiol. (Praha), vol. 49, pp. 191-193."
],
"prototype": "MboI",
"organism": "Megasphaera elsedenii 4O",
"methylation_site": ""
},
"srr17i": {
"target_site": "ATTAAT",
"name": "Srr17I",
"suppliers": [],
"source": "I. Vanat",
"references": [
"Zatkovic, B., Molnarova, V., Kmet, V., Javorsky, P., Pristas, P., (2000) Anaerobe, vol. 6, pp. 299-304."
],
"prototype": "VspI",
"organism": "Selenomonas ruminantium 17",
"methylation_site": ""
},
"esp8i": {
"target_site": "GCGCGC",
"name": "Esp8I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Bitinaite, J., Grigaite, R., Jagelavicius, M., Unpublished observations."
],
"prototype": "BsePI",
"organism": "Erwinia species RFL8",
"methylation_site": ""
},
"m.ssp6803dam": {
"target_site": "GATC",
"name": "M.Ssp6803Dam",
"suppliers": [],
"source": "PCC 6803",
"references": [
"Ahlert, D., Stegemann, S., Kahlau, S., Ruf, S., Bock, R., (2009) Mol. Genet. Genomics, vol. 282, pp. 17-24.",
"Kaneko, T. et al., (1996) DNA Res., vol. 3, pp. 185-209.",
"Roberts, R.J., Unpublished observations."
],
"prototype": "MboI",
"organism": "Synechocystis sp. PCC 6803",
"methylation_site": "?(6)"
},
"pmni": {
"target_site": "GGCGCC",
"name": "PmnI",
"suppliers": [],
"source": "V.E. Repin",
"references": [
"Repin, V.E., Puchkova, L.I., Rodicheva, E.K., Vydryakova, G.A., (1997) Prikl. Biokhim. Mikrobiol., vol. 33, pp. 152-155."
],
"prototype": "NarI",
"organism": "Photobacterium mandapamensis 579",
"methylation_site": ""
},
"ttoi": {
"target_site": "CCGCGG",
"name": "TtoI",
"suppliers": [],
"source": "K. Inagaki",
"references": [
"Inagaki, K., Morimoto, Y., Hiraoka, N., Sugio, T., Tano, T., Unpublished observations."
],
"prototype": "SacII",
"organism": "Thiobacillus thiooxidans ON106",
"methylation_site": ""
},
"axyi": {
"target_site": "CC^TNAGG",
"name": "AxyI",
"suppliers": [
"Nippon Gene Co., Ltd. (1/11)"
],
"source": "IFO 3288",
"references": [
"Yoshioka, H., Nakamura, H., Sasaki, J., Tahara, Y., Yamada, Y., (1983) Agric. Biol. Chem., vol. 47, pp. 2871-2879."
],
"prototype": "SauI",
"organism": "Acetobacter xylinus",
"methylation_site": ""
},
"bme899i": {
"target_site": "?",
"name": "Bme899I",
"suppliers": [],
"source": "IPP 899",
"references": [
"Shibata, T., Ikawa, S., Kim, C., Ando, T., (1976) J. Bacteriol., vol. 128, pp. 473-476."
],
"prototype": "",
"organism": "Bacillus megaterium 899",
"methylation_site": ""
},
"asp742i": {
"target_site": "GGCC",
"name": "Asp742I",
"suppliers": [],
"source": "C. Kessler",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "HaeIII",
"organism": "Achromobacter species 742",
"methylation_site": ""
},
"eco1158i": {
"target_site": "TGANNNNNNNNTGCT",
"name": "Eco1158I",
"suppliers": [],
"source": "J.-I. Ryu",
"references": [
"Kasarjian, J.K.A., Iida, M., Ryu, J., (2003) Nucleic Acids Res., vol. 31."
],
"prototype": "EcoBI",
"organism": "Escherichia coli EC1158",
"methylation_site": "?(6)"
},
"cfr28i": {
"target_site": "CCWGG",
"name": "Cfr28I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Lazareviciute, L., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii RFL28",
"methylation_site": ""
},
"bsp103i": {
"target_site": "CCWGG",
"name": "Bsp103I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Bacillus species RFL103",
"methylation_site": ""
},
"llaioi": {
"target_site": "?",
"name": "LlaIOI",
"suppliers": [],
"source": "T. Suzuki",
"references": [
"Yasui, K., Kano, Y., Tanaka, K., Watanabe, K., Shimizu-Kadota, M., Yoshikawa, H., Suzuki, T., (2009) Nucleic Acids Res., vol. 37."
],
"prototype": "",
"organism": "Lactococcus lactis IO-1",
"methylation_site": ""
},
"uba1311i": {
"target_site": "CCWWGG",
"name": "Uba1311I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "StyI",
"organism": "Unidentified bacterium RFL1311",
"methylation_site": ""
},
"ngodxiv": {
"target_site": "GATC",
"name": "NgoDXIV",
"suppliers": [],
"source": "J.K. Davies",
"references": [
"Duff, M.K., Davies, J.K., (1988) Gonococci and Meningococci, ed. Poolman, J.T., Zanen, H.C., Meyer, T.F., Heckels, J.E., Makela, P.R.H. (Kluwer Academic Publishers, Dordrecht, The Netherlands), pp. 251-256."
],
"prototype": "DpnI",
"organism": "Neisseria gonorrhoeae JKD211",
"methylation_site": ""
},
"sspki": {
"target_site": "CGTACG",
"name": "SspKI",
"suppliers": [],
"source": "M. Sakakibara",
"references": [
"Kumano, M., Sakakibara, M., (1988) Baiosaiensu to Indasutori, vol. 46, pp. 38-41."
],
"prototype": "SplI",
"organism": "Spirulina species K",
"methylation_site": ""
},
"bstapi": {
"target_site": "GCANNNN^NTGC",
"name": "BstAPI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 1778",
"references": [
"Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh., (1997) Nucleic Acids Res., vol. 25, pp. 2301-2302.",
"Zhu, Z., Roberts, R.J., Unpublished observations."
],
"prototype": "ApaBI",
"organism": "Bacillus stearothermophilus AP",
"methylation_site": ""
},
"sacni": {
"target_site": "GRGCY^C",
"name": "SacNI",
"suppliers": [],
"source": "A.J. Podhajska",
"references": [
"Rutkowska, S.M., Skowron, P.M., Bielawski, K., Podhajska, A.J., (1995) Gene, vol. 157, pp. 319-320."
],
"prototype": "HgiJII",
"organism": "Streptomyces achromogenes N-J-H",
"methylation_site": ""
},
"bth2350i": {
"target_site": "CAGCTG",
"name": "Bth2350I",
"suppliers": [],
"source": "NEB 1397",
"references": [
"Nkenfou, C.N., Nkenfou, J., Unpublished observations."
],
"prototype": "PvuII",
"organism": "Bacillus thuringiensis 2350",
"methylation_site": ""
},
"bnai": {
"target_site": "G^GATCC",
"name": "BnaI",
"suppliers": [],
"source": "E.L. Kim",
"references": [
"Kim, E.L., Maliuta, S.S., (1989) Gene, vol. 80, pp. 363-368.",
"Kim, E.L., Maliuta, S.S., (1989) FEBS Lett., vol. 255, pp. 361-364."
],
"prototype": "BamHI",
"organism": "Bacillus natto B3364",
"methylation_site": "5(5)"
},
"eco81i": {
"target_site": "CC^TNAGG",
"name": "Eco81I",
"suppliers": [
"Fermentas International Inc. (1/11)",
"Takara Bio Inc. (1/11)",
"Toyobo Biochemicals (9/09)"
],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Butkus, V., Petrauskiene, L., Petrusyte, M., Unpublished observations."
],
"prototype": "SauI",
"organism": "Escherichia coli RFL81",
"methylation_site": ""
},
"fisi": {
"target_site": "CTAG",
"name": "FisI",
"suppliers": [],
"source": "DSM 5733",
"references": [
"Andrews, K.T., Patel, B.K.C., Clarke, F.M., (1998) Anaerobe, vol. 4, pp. 227-232."
],
"prototype": "MaeI",
"organism": "Fervidobacterium islandicum H21",
"methylation_site": ""
},
"eco159i": {
"target_site": "GAATTC",
"name": "Eco159I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "EcoRI",
"organism": "Escherichia coli RFL159",
"methylation_site": ""
},
"nrugi": {
"target_site": "GACNNN^NNGTC",
"name": "NruGI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Bondar, T.S., Rechkunova, N.I., Abdurashitov, M.A., Dedkov, V.S., Degtyarev, S.K., Unpublished observations.1"
],
"prototype": "Eam1105I",
"organism": "Nocardia species G",
"methylation_site": ""
},
"mmyci": {
"target_site": "?",
"name": "MmyCI",
"suppliers": [],
"source": "J.I. Glass",
"references": [
"Lartigue, C., Vashee, S., Algire, M.A., Chuang, R.Y., Benders, G.A., Ma, L., Noskov, V.N., Denisova, E.A., Gibson, D.G., Assad-Garcia, N., Alperovich, N., Thomas, D.W., Merryman, C., Hutchison, C.A.I.I.I., Smith, H.O., Venter, J.C., Glass, J.I., (2009) Science, vol. 325, pp. 1693-1696."
],
"prototype": "",
"organism": "Mycoplasma mycoides subsp. capri str. GM12",
"methylation_site": "?(6)"
},
"truii": {
"target_site": "GATC",
"name": "TruII",
"suppliers": [],
"source": "BKM B-1258",
"references": [
"Bernal, W.M., Raven, N.D.H., Williams, R.A.D., (1986) Proceedings 14th Int. Congress Microbiol., vol. 0, pp. 204."
],
"prototype": "MboI",
"organism": "Thermus ruber strain 21",
"methylation_site": ""
},
"m.bbvsi": {
"target_site": "GCWGC",
"name": "M.BbvSI",
"suppliers": [],
"source": "A.P. Zarubina",
"references": [
"Vanyushin, B.F., Dobritsa, A.P., (1975) Biochim. Biophys. Acta, vol. 407, pp. 61-72."
],
"prototype": "TseI",
"organism": "Bacillus brevis S",
"methylation_site": "2(5)"
},
"hpyf6iii": {
"target_site": "CTNAG",
"name": "HpyF6III",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "DdeI",
"organism": "Helicobacter pylori RFL6",
"methylation_site": ""
},
"cjai": {
"target_site": "CTCGAG",
"name": "CjaI",
"suppliers": [],
"source": "ATCC 31637",
"references": [
"Hamablet, L., Meyertons, J.L., Roberts, R.J., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Catenuloplanes japonicus",
"methylation_site": ""
},
"sru30di": {
"target_site": "AGG^CCT",
"name": "Sru30DI",
"suppliers": [],
"source": "P. Pristas",
"references": [
"Pristas, P., Vanat, I., Javorsky, P., (1995) Gene, vol. 158, pp. 139-140."
],
"prototype": "StuI",
"organism": "Selenomonas ruminantium 30",
"methylation_site": ""
},
"mspswi": {
"target_site": "ATTT^AAAT",
"name": "MspSWI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Abdurashitov, M.A., Dedkov, V.S., Bondar, T.S., Shevchenko, A.V., Degtyarev, S.K., Unpublished observations."
],
"prototype": "SwaI",
"organism": "Moraxella species SW",
"methylation_site": ""
},
"eco135i": {
"target_site": "CCGCGG",
"name": "Eco135I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Steponaviciene, D., Unpublished observations."
],
"prototype": "SacII",
"organism": "Escherichia coli RFL135",
"methylation_site": ""
},
"alwfii": {
"target_site": "CTCGAG",
"name": "AlwFII",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Nekrasiene, D., Lapcinskaja, S., Kiuduliene, L., Vitkute, J., Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Acinetobacter lwoffii RFL45",
"methylation_site": ""
},
"xcpi": {
"target_site": "CTGCA^G",
"name": "XcpI",
"suppliers": [],
"source": "S.K. Degtyarev",
"references": [
"Ra, S.R., Kim, H.S., Kim, Y.G., Dedkov, V.S., Degtyarev, S.K., Unpublished observations."
],
"prototype": "PstI",
"organism": "Xanthomonas campestris 723",
"methylation_site": ""
},
"hpyf54i": {
"target_site": "ACNGT",
"name": "HpyF54I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "Tsp4CI",
"organism": "Helicobacter pylori RFL54",
"methylation_site": ""
},
"tsebi": {
"target_site": "GCWGC",
"name": "TseBI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Jones, S., Hill, V.J., Vincent, S., Clark, D.R., Unpublished observations."
],
"prototype": "TseI",
"organism": "Thermus species Azores9539P",
"methylation_site": ""
},
"uban10i": {
"target_site": "WCCGGW",
"name": "UbaN10I",
"suppliers": [],
"source": "J. Huete-Perez",
"references": [
"Roustan-Espinosa, I., Guerrero, D., Flores, E., Huete-Perez, J., (2000) Revista Univ. Centroamer. (Revista de la Universidad Centroamericana), vol. 52, pp. 10-18."
],
"prototype": "BetI",
"organism": "Unidentified bacterium M4-1ATM, Nicaragua",
"methylation_site": ""
},
"fati": {
"target_site": "^CATG",
"name": "FatI",
"suppliers": [
"SibEnzyme Ltd. (1/11)",
"New England Biolabs (1/11)"
],
"source": "NEB 1779",
"references": [
"Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K., (2002) Biotekhnologiya, vol. 5, pp. 3-7.",
"Morgan, R.D., Unpublished observations."
],
"prototype": "NlaIII",
"organism": "Flavobacterium aquatile NL3",
"methylation_site": "?(5)"
},
"bspt107i": {
"target_site": "G^GYRCC",
"name": "BspT107I",
"suppliers": [
"Takara Bio Inc. (1/11)"
],
"source": "Takara T107",
"references": [
"Sawaragi, H., Unpublished observations."
],
"prototype": "HgiCI",
"organism": "Bacillus species T107",
"methylation_site": ""
},
"sinci": {
"target_site": "GGWCC",
"name": "SinCI",
"suppliers": [],
"source": "S. Horiuchi",
"references": [
"Miyahara, M., Mise, K., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Salmonella infantis 85005",
"methylation_site": ""
},
"bcci": {
"target_site": "CCATC(4/5)",
"name": "BccI",
"suppliers": [
"New England Biolabs (1/11)"
],
"source": "NEB 669",
"references": [
"Morgan, R., Reinecke, S., Unpublished observations.",
"Morgan, R.D., Unpublished observations.",
"Morgan, R.D., Roberts, R.J., International Patent Office, 2007."
],
"prototype": "",
"organism": "Bacteroides caccae",
"methylation_site": ""
},
"bshhi": {
"target_site": "AGTACT",
"name": "BshHI",
"suppliers": [],
"source": "D. Clark",
"references": [
"Clark, D., Unpublished observations."
],
"prototype": "ScaI",
"organism": "Bacillus sphaericus DC1",
"methylation_site": ""
},
"bimi": {
"target_site": "TT^CGAA",
"name": "BimI",
"suppliers": [],
"source": "G.G. Prikhodko",
"references": [
"Morgan, R.D., Unpublished observations.",
"Prichodko, G.G., Degtyarev, S.K., Rechkunova, N.I., Sosnovsev, S.V., Tchigikov, V.E., (1990) Biotekhnologiya, vol. 1, pp. 12-16."
],
"prototype": "AsuII",
"organism": "Brevibacterium immotum",
"methylation_site": ""
},
"msii": {
"target_site": "CTCGAG",
"name": "MsiI",
"suppliers": [],
"source": "H. Reichenbach",
"references": [
"Middleton, J.H., Stankus, P.V., Edgell, M.H., Hutchison, C.A. III, Unpublished observations.",
"Myers, P.A., Roberts, R.J., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Myxococcus stipitatus Mxs2",
"methylation_site": ""
},
"bscai": {
"target_site": "GCATC(4/6)",
"name": "BscAI",
"suppliers": [],
"source": "CAMB 2585",
"references": [
"Choudhry, S., Sohail, A., Malik, K., Riazuddin, S., Unpublished observations.",
"Zhu, Z., Unpublished observations."
],
"prototype": "SfaNI",
"organism": "Bacillus schlegelii S3",
"methylation_site": ""
},
"uba1166i": {
"target_site": "CTCGAG",
"name": "Uba1166I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Unpublished observations."
],
"prototype": "XhoI",
"organism": "Unidentified bacterium RFL1166",
"methylation_site": ""
},
"bli1904ii": {
"target_site": "GCNGC",
"name": "Bli1904II",
"suppliers": [],
"source": "M. Rey",
"references": [
"Berka, R., Rey, M., Ramaiya, P., International Patent Office, 2008.",
"Thomas, M., Rey, M., International Patent Office, 2008."
],
"prototype": "Fnu4HI",
"organism": "Bacillus licheniformis SJ1904",
"methylation_site": "?(5)"
},
"ecl37kii": {
"target_site": "CCWGG",
"name": "Ecl37kII",
"suppliers": [],
"source": "A.S. Solonin",
"references": [
"Sineva, E.V., Zakharova, G.G., Tarutina, Z.E., Kravetz, A.N., Solonin, A.S., (1993) Mol. Gen. Mikrobiol. Virusol., vol. 0, pp. 10-13."
],
"prototype": "EcoRII",
"organism": "Enterobacter cloacae 37k",
"methylation_site": ""
},
"bst170i": {
"target_site": "TGTACA",
"name": "Bst170I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "Bsp1407I",
"organism": "Bacillus stearothermophilus D170",
"methylation_site": ""
},
"cfrs37i": {
"target_site": "CCWGG",
"name": "CfrS37I",
"suppliers": [],
"source": "W. Piepersberg",
"references": [
"Bolton, B.J., Comer, M.J., Kessler, C., Unpublished observations."
],
"prototype": "EcoRII",
"organism": "Citrobacter freundii S39",
"methylation_site": ""
},
"ssp1725i": {
"target_site": "CCGCGG",
"name": "Ssp1725I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "SacII",
"organism": "Streptomyces spadicis 1725",
"methylation_site": ""
},
"eco72i": {
"target_site": "CAC^GTG",
"name": "Eco72I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Kazlauskiene, R., Maneliene, Z., Butkus, V., Petrusyte, M., Janulaitis, A., (1986) Bioorg. Khim., vol. 12, pp. 836-838."
],
"prototype": "PmaCI",
"organism": "Escherichia coli RFL72",
"methylation_site": ""
},
"m.smei": {
"target_site": "GANTC",
"name": "M.SmeI",
"suppliers": [],
"source": "L. Shapiro",
"references": [
"Benkovic, S.J., Shapiro, L., Wright, R., Stephens, C., Kahng, L.Sue., Berdis, A., Lee, I., US Patent Office, 2005.",
"Galibert, F. et al., (2001) Science, vol. 293, pp. 668-672.",
"Wright, R., Stephens, C., Shapiro, L., (1997) J. Bacteriol., vol. 179, pp. 5869-5877."
],
"prototype": "HinfI",
"organism": "Sinorhizobium meliloti",
"methylation_site": "2(6)"
},
"hpyf14i": {
"target_site": "CGCG",
"name": "HpyF14I",
"suppliers": [],
"source": "V. Butkus",
"references": [
"Vitkute, J., Petrusyte, V., Trinkunaite, L., Kiuduliene, L., Janulaitis, A., Unpublished observations."
],
"prototype": "FnuDII",
"organism": "Helicobacter pylori RFL14",
"methylation_site": ""
},
"atu1ii": {
"target_site": "GGATCC",
"name": "Atu1II",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Kazlauskiene, R., Petrusyte, M., Unpublished observations."
],
"prototype": "BamHI",
"organism": "Agrobacterium tumefaciens RFL1",
"methylation_site": ""
},
"bspf53i": {
"target_site": "GGWCC",
"name": "BspF53I",
"suppliers": [],
"source": "Z. Chen",
"references": [
"Chen, Z., Unpublished observations."
],
"prototype": "AvaII",
"organism": "Bacillus species cy53",
"methylation_site": ""
},
"m.pmudam": {
"target_site": "GATC",
"name": "M.PmuDam",
"suppliers": [],
"source": "V. Kapur",
"references": [
"Chen, L., Paulsen, D.B., Lawrence, M.L., Unpublished observations.",
"May, B.J., Zhang, Q., Li, L.L., Paustian, M.L., Whittam, T.S., Kapur, V., (2001) Proc. Natl. Acad. Sci. U. S. A., vol. 98, pp. 3460-3465."
],
"prototype": "MboI",
"organism": "Pasteurella multocida Pm70",
"methylation_site": "?(6)"
},
"eco147i": {
"target_site": "AGG^CCT",
"name": "Eco147I",
"suppliers": [
"Fermentas International Inc. (1/11)"
],
"source": "A. Janulaitis",
"references": [
"Gilvonauskaite, R., Maneliene, Z., Padegimiene, A., Petrusyte, M., Janulaitis, A., Unpublished observations.",
"Janulaitis, A., Gilvonauskaite, R., Petrusyte, M., Unpublished observations.",
"Stankevicius, K., Povilionis, P., Lubys, A., Menkevicius, S., Janulaitis, A., (1995) Gene, vol. 157, pp. 49-53."
],
"prototype": "StuI",
"organism": "Escherichia coli RFL147",
"methylation_site": ""
},
"vpak8ai": {
"target_site": "?",
"name": "VpaK8AI",
"suppliers": [],
"source": "K. Mise",
"references": [
"Miyahara, M., Fujiwara, R., Mise, K., Shimada, T., Matsushita, S., Kudoh, Y., Ishiwata, N., Tanimura, A., (1994) J. Food. Hyg. Sci. Japan, vol. 35, pp. 605-609."
],
"prototype": "",
"organism": "Vibrio parahaemolyticus TVP1764",
"methylation_site": ""
},
"pph3215i": {
"target_site": "GWGCWC",
"name": "Pph3215I",
"suppliers": [],
"source": "U. Mayr",
"references": [
"Kaluza, K., Schmitz, G., Jarsch, M., Herz, G., Wagner, C., Veseley, Z., Berger, G., Kessler, C., Unpublished observations."
],
"prototype": "HgiAI",
"organism": "Pseudomonas phaseolicola 3215",
"methylation_site": ""
},
"m.tthbi": {
"target_site": "?",
"name": "M.TthBI",
"suppliers": [],
"source": "S. Hattman",
"references": [
"Bromberg, S., Pratt, K., Hattman, S., (1982) J. Bacteriol., vol. 150, pp. 993-996."
],
"prototype": "",
"organism": "Tetrahymena thermophila B-1868-VII",
"methylation_site": "?(6)"
},
"eco191i": {
"target_site": "GGTCTC",
"name": "Eco191I",
"suppliers": [],
"source": "A. Janulaitis",
"references": [
"Janulaitis, A., Gilvonauskaite, R., Unpublished observations."
],
"prototype": "Eco31I",
"organism": "Escherichia coli RFL191",
"methylation_site": ""
},
"rmei": {
"target_site": "?",
"name": "RmeI",
"suppliers": [],
"source": "J.L. Denarie",
"references": [
"Heumann, W., (1979) Curr. Top. Microbiol. Immunol., vol. 88, pp. 1-24."
],
"prototype": "",
"organism": "Rhizobium meliloti",
"methylation_site": ""
},
"uba1393i": {
"target_site": "CCCGGG",
"name": "Uba1393I",
"suppliers": [],
"source": "A. Janulaitis",
@vedtopkar
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This is so useful, thanks! 😄

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