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cjfields/no samblaster

Last active August 29, 2015 14:05
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samblaster problem
Raw
no samblaster
-system-specific-4.1$ samtools view -h Control_Input_2_GAGTGG_L008_R1_001.trimmed.sorted.bam | head -n 100
@HD VN:1.0 SO:coordinate
@PG ID:novoalign PN:novoalign VN:V3.02.00 CL:novoalign -c 7 -d /home/mirrors/igenome/Homo_sapiens/UCSC/hg19/Sequence/NovoalignIndex/WholeGenomeFasta.nvi -f /home/groups/hpcbio/projects/longley/2014-08-04/results/2014-08-06-alignment/Control_Input_2_GAGTGG_L008_R1_001.trimmed.fastq.gz -l 15 -r RANDOM -o SAM
@SQ SN:chrM LN:16571 AS:WholeGenomeFasta.nvi
@SQ SN:chr1 LN:249250621 AS:WholeGenomeFasta.nvi
@SQ SN:chr2 LN:243199373 AS:WholeGenomeFasta.nvi
@SQ SN:chr3 LN:198022430 AS:WholeGenomeFasta.nvi
@SQ SN:chr4 LN:191154276 AS:WholeGenomeFasta.nvi
@SQ SN:chr5 LN:180915260 AS:WholeGenomeFasta.nvi
@SQ SN:chr6 LN:171115067 AS:WholeGenomeFasta.nvi
@SQ SN:chr7 LN:159138663 AS:WholeGenomeFasta.nvi
@SQ SN:chr8 LN:146364022 AS:WholeGenomeFasta.nvi
@SQ SN:chr9 LN:141213431 AS:WholeGenomeFasta.nvi
@SQ SN:chr10 LN:135534747 AS:WholeGenomeFasta.nvi
@SQ SN:chr11 LN:135006516 AS:WholeGenomeFasta.nvi
@SQ SN:chr12 LN:133851895 AS:WholeGenomeFasta.nvi
@SQ SN:chr13 LN:115169878 AS:WholeGenomeFasta.nvi
@SQ SN:chr14 LN:107349540 AS:WholeGenomeFasta.nvi
@SQ SN:chr15 LN:102531392 AS:WholeGenomeFasta.nvi
@SQ SN:chr16 LN:90354753 AS:WholeGenomeFasta.nvi
@SQ SN:chr17 LN:81195210 AS:WholeGenomeFasta.nvi
@SQ SN:chr18 LN:78077248 AS:WholeGenomeFasta.nvi
@SQ SN:chr19 LN:59128983 AS:WholeGenomeFasta.nvi
@SQ SN:chr20 LN:63025520 AS:WholeGenomeFasta.nvi
@SQ SN:chr21 LN:48129895 AS:WholeGenomeFasta.nvi
@SQ SN:chr22 LN:51304566 AS:WholeGenomeFasta.nvi
@SQ SN:chrX LN:155270560 AS:WholeGenomeFasta.nvi
@SQ SN:chrY LN:59373566 AS:WholeGenomeFasta.nvi
HWI-ST330:434:C50N2ACXX:8:1101:1454:8597 0 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA @@@DADBAFFHHGIA@A<AHFE@EEGGHEEHIEHGIGHGHA0@FGHII@CFHGCD;CDGGIFIIE@<=1;;>>ADB09BDCD@?BBDB9>CADD>CB<BD PG:Z:novoalign AS:i:70 UQ:i:70 NM:i:0 MD:Z:95
HWI-ST330:434:C50N2ACXX:8:1101:7050:47300 16 chrM 1 51 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC A@>:CCCA?BC@444:::4?DDCC@>CB<:@ADDDC><:7AAB<BA>CCBFDEFEAAEIGHHD=DGIGCIJIGHEFDEBHEDIJIIGBHGHHFFDFFCCC PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1101:10175:66904 0 chrM 1 70 2S98M * 0 0 TGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCTAGACGC CCCFFFFFHHHFHIJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJFHJJJJJJJJJJIJHFD>@B@DEDDDDDDDDDDEDDDDDDDDDD PG:Z:novoalign AS:i:112 UQ:i:112 NM:i:2 MD:Z:33G57G6
HWI-ST330:434:C50N2ACXX:8:1101:4571:71162 0 chrM 1 53 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC @@@DDDDA<FDAFIIFIIFIEBF+ACHB?EGFGFIFE>G9BGFEIEFIEFIFFAAFD@@=@D?7A=A>(;?BDDD>CCB?=@BBBBB@?@>@?>>B>>9> PG:Z:novoalign AS:i:125 UQ:i:125 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1101:6927:74501 0 chrM 1 37 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA @C@FDDDFBDFH<@GBGGIEHGGGGHGIEHGHGHIIGCHGGEGB@FHGIGHGHGGIGGEGICHAHF@HEB;;@@C>3=A@A?C?BBB5>>BCAC@B@>BB PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1102:15629:92216 0 chrM 1 70 3S97M * 0 0 ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACG CCCFFFFFHHHHBFGGIJJJJJJIJJIJJJIIJJJIIJJGIIIJJJJIIJFJJJJJJHIIIEIIIIHHHHFDDD&0<@CDDDDBDDDBDC<ACCDD>>>B PG:Z:novoalign AS:i:60 UQ:i:60 NM:i:0 MD:Z:97
HWI-ST330:434:C50N2ACXX:8:1103:20430:15560 0 chrM 1 70 1S99M * 0 0 GGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGGGTGCACGCGATAGCATTGCGAGACGCT ?@@DDDDDH=F?DHBH?GCCEGHH>HEEBEHGHIEHEDD:GGBG@GHCD@EDFF@F>@@@FHB=;??9ADC9&0552>>?59&500@@>C:35<5><859 PG:Z:novoalign AS:i:58 UQ:i:58 NM:i:1 MD:Z:71T27
HWI-ST330:434:C50N2ACXX:8:1104:4867:14179 0 chrM 1 49 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG CCCFFFFFHHHHHJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJIJGIJJIJJJIIHHHHGHHBDFFFFEDDDDDDDDDD5@9@DEDDDDDDDD PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1107:15210:47475 16 chrM 1 70 6S94M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAG <DDDCC>EDCCDDDDDDEDBBADDDEDDC?>BC@DBB?DDDDDDDCEEEEFFFFFFHFHHHJIHHHIJJGJIGJJJJIIJIJIGIJJHHGHHFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:94
HWI-ST330:434:C50N2ACXX:8:1107:14783:91196 16 chrM 1 70 6S94M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAG <DDDDDEEEDDDDDDDDEDB@CDCCDDDC@>BA>DBBADBCDDDDEEDEDFFFFFFHHHHHEJJJJIIJIJJGIJIJJJJJIIIJJJHHHHGFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:94
HWI-ST330:434:C50N2ACXX:8:1108:11963:23338 16 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC CDCDC??B<?CCA@A>@ACDCACC>CC??:DDC@>>A?@BA>?;BCCA@>CEECC?HAG@HECDGIHHCFAF@:HEFB<GHEIGIEGDHFDDDBDDF@@C PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1108:12666:26284 0 chrM 1 5 22S67M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGG ==?=DDADDD:4<C:<A<AF;<CDFEEEE<?*?CB99?DC?B3:9DDEBEDD?DDAAAAD<CE7;AA;AADDA@AA@66(6>;85==?? PG:Z:novoalign AS:i:174 UQ:i:174 NM:i:0 MD:Z:67 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1108:6950:92099 0 chrM 1 37 17S82M1S * 0 0 AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGAG CCCFFFDFGHHHHJIJIJIJIJIJIJFHIIJJJJJJJJJJJJJJJJFIIJJIHIIJFGIJJIHHGEHHFFF;@DE>CEC?BDDDDDDD5<<?CEDDDD<@ PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1109:15878:2540 16 chrM 1 70 9S91M * 0 0 ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGC C?BADDDDDEEEDDDDDDDDEDDBDBDDDDCC?>DCCBDB?@DEEEDDEFFFHHHHGHJJJJJJJJJIGIIJJIJIGJJJGHBGHHIGHHHHFFFFFCCC PG:Z:novoalign AS:i:94 UQ:i:94 NM:i:0 MD:Z:91
HWI-ST330:434:C50N2ACXX:8:1109:18041:72542 0 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG @@@DDDFFGDHFDGIIIGDGGJBGGIGHIG9CFHDFCHHIDGHIJIGBDHIIIGIFEHI>AEDCEHHHFBEC?BDA;CEFEEBBDDDCC::@5<9@CCDD PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1110:8402:1988 16 chrM 1 44 16S82M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGA DEDEDDDDDDBDDDDDEEEDDDDDCDDEDD@CDDDEECCBADCADDDADFFFFHHHHJJIJJJJIJJJJJGJJJJJJJIJJJJJJIJHHGHHDFFFD= PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1110:18908:5612 16 chrM 1 70 11S89M * 0 0 ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATT CDDDDDDCDDDEEEDDDDDDDCBAA<9BCDDDCDB>DCCB?DBDDEEDFFEFHHHHHHJJJJJJJJJIHJJIJJJIJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:106 UQ:i:106 NM:i:0 MD:Z:89
HWI-ST330:434:C50N2ACXX:8:1110:11611:66991 16 chrM 1 70 8S92M * 0 0 TCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCG CBADDDDDDEEDDDDDDDDEDB??BDDEECCBADA>DBBADDDECDDCFFFDFFEHEHFJJJJIJJJJIIIJJJJJJJJJJJJIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:88 UQ:i:88 NM:i:0 MD:Z:92
HWI-ST330:434:C50N2ACXX:8:1111:19544:79011 0 chrM 1 70 4S96M * 0 0 GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC @??;ADDD?CFA=<CDFCCEHGHGEGHIHIIIHGGGHB@D)8@BFGHI>FBGFHEFCD6@CED@@G?AHEEEDCB39<<CDCBBBBBBBCCDCCCBBBBB PG:Z:novoalign AS:i:65 UQ:i:65 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1112:8119:19174 0 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC CCCFFFFFHHHHHJJJIIJIJJJJJJJIJJJGHIIJIJJJJJJJJJIJJJJJJJJJGHGIJJIJJJJJJJJHHHHCEEFFFFFDDDDDDDDDD>BBBDED PG:Z:novoalign AS:i:202 UQ:i:202 NM:i:1 MD:Z:33G44
HWI-ST330:434:C50N2ACXX:8:1113:1327:15419 0 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG ?:=DD?DDBFFDFEI@FG=<<ACHHEE@FFFIIDFBB?FF>BBG@FFI>BAC=@FIEE>@DEEE=CE;>;;BB?@=;=BBBB@<?BDABB9>@BB>BEBB PG:Z:novoalign AS:i:100 UQ:i:100 NM:i:0 MD:Z:90
HWI-ST330:434:C50N2ACXX:8:1113:2480:74049 0 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CC@FFFADFHGHFIBAGHHGGCHHIEHIIIIIHICF@GGCGIC<GG9?BGG@AE8FEDDEA4).7AEHFF;?B:A>><6,;??CDD@&05?CA>?@>>B< PG:Z:novoalign AS:i:139 UQ:i:139 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1114:5422:16432 0 chrM 1 24 19S81M * 0 0 TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGGGTGCACGCG @@@DD?DA?BFDDGAFBHH<??FG;9EDGHB<EFCFFGHGHAHGA<<DGHIG;6:/;ACCAHHA>CAHC?BEE;@A>>AC;5;?=??/25&5<@B4A<9< PG:Z:novoalign AS:i:164 UQ:i:164 NM:i:1 MD:Z:71T9
HWI-ST330:434:C50N2ACXX:8:1114:12343:64992 0 chrM 1 45 15S83M2S * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGAGATCG ?;7?;DDDDDADCEEEE4?:<ADEE9A<E<4CE;CC*:*?9?*:?9?DDDD0-@AD7AC77=??C33;>;3?AD;@86;=;9=?=>&0)2>A:>&050>? PG:Z:novoalign AS:i:176 UQ:i:176 NM:i:1 MD:Z:79C3
HWI-ST330:434:C50N2ACXX:8:1115:5883:14312 0 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG @@@DFFFDHDFDDFFHIIEE=<E<CGGGIIECHGGHCGGGI@DHCGHIIIII@FH@@FEGGIFFFGFHFFFECEF=;@CCDEB??B@CCB:<09<?C>C< PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1115:7853:81846 16 chrM 1 70 96M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC BBA:43:::4:4+(++8B>:44+84<>3BBB?88,(-(=B@CC@DB>D@B?7=3C>B<BAFFIFF?@D?D8G@F@?)FF:BIFFFDF?@??AD@@? PG:Z:novoalign AS:i:4 UQ:i:4 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1201:4192:2192 16 chrM 1 70 11S89M * 0 0 ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCAATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATT BC?9@ADDCDCDDC>4DDDDDC@AA?9BDCC@A:?:?>>DB>;DDEEFFFFEHCGHHHGIHC@GJJJIGJIGGJHFJIGFIEJJJIGFHHHHFDFFFCCC PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:1 MD:Z:43C45
HWI-ST330:434:C50N2ACXX:8:1201:13214:10220 16 chrM 1 47 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG :AD@@CAC?<@>CCDDCCDDCDDDCCDC?8,@CCCDC@85B>5?=?;CBEDDBEAE>HEAIEDIIJJJIHAIGGHHHEJJJIIJJJJHHHFFFDDFFC@C PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1201:10956:28155 16 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG ::::@AA>:3>DC8@?DDDDDA>2:?:4?<9?B@CAABDDBCB@>@C@>C@CECB>BHB<GGFFBJIGF?BEF?BFEGJJJJGIGGGFAAABFFFFFCCC PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1201:1702:33608 0 chrM 1 70 3S97M * 0 0 ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACG @@@DFFFFFHHHCEGIGJGIJJGGIIECHI>;EEHHGGFIGHGICGHGGGIBGIIJJ=@FEGBGGHCHFFFFDD59<?CCCD>B<B@<A>CCDDDDD>B@ PG:Z:novoalign AS:i:58 UQ:i:58 NM:i:0 MD:Z:97
HWI-ST330:434:C50N2ACXX:8:1201:3679:37230 0 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG CCCFFFFFGGDHHGIGIICEHIIIIGIJJJCGHGGIIHIIJIIIIJJIJIJJJJGHBHHJJIIHHHHHDBDFDFBAACDEEEDDDDDDDBBD&5&8@DDD PG:Z:novoalign AS:i:170 UQ:i:170 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1202:11253:16296 0 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG CCCFFFFFHFHHHJJJJJJJJJJIJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJGIJJJJJJHIJHHHHDD;@BBCDDDDDDDDDCDEDCDDDDDDDDDD PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1202:16782:30526 0 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG @CCFFFFFFCDFDIIJJJJJIJJIGIIJJIJIGGIIJJJJJJJJJGGGIIJIIJFGGIJIEHHIJHHHGDD9@5?:>CDBDB<BBDDECCDDBBBB?B<@ PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1203:16581:7133 0 chrM 1 32 18S82M * 0 0 AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGA CCCFFDEDHGHHHJJJBHHIEFEGHIJFFH9FHIGIGHHIGIIIIJJJJCHIIIIHJJJGIJEEHHHHFFFFBFDCDDD@;?ADCDDDD5@5?CDDD>B> PG:Z:novoalign AS:i:148 UQ:i:148 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1203:2051:23459 0 chrM 1 70 8S92M * 0 0 TCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCCCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCG @@@FDFFFGHHHHJJGB?EHIIJIIJGEGIFGGEIIJFF>FIFJ@F:FHI@@AGIIGIGIIG=?EHAEFFDCCBCEBDD@B5?CDCBB@>BBDDDEDDDD PG:Z:novoalign AS:i:118 UQ:i:118 NM:i:1 MD:Z:41T50
HWI-ST330:434:C50N2ACXX:8:1203:11529:37247 16 chrM 1 38 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA C@CA>BCCCAA?CCCCED@>434:>CCDCA<,BCCDACC<5=;5B?A=A?C;>EA>?@DECIIIIHEEIGFBGD7?ADB7:HIHIIG>FDFD?DDDD@@@ PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1204:16260:97414 0 chrM 1 70 4S96M * 0 0 GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC ?=?BDBADFBFA32?AE>:CBCFBB@HB9CHF@DGFIIF6?F0?F<8;/BC==4CFAFFGIFEEEBCEE;7)9>@-55?A4:?9<@909>>4(>AB3509 PG:Z:novoalign AS:i:66 UQ:i:66 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1205:2637:15505 0 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA CCCFFFFDHHHHFIGHGIJJIIIIJIIIJGIIGGHHGIJJJJJIHGGIGCGIJIBGGGGGGGHHHHGDDFCCFDEDCCECED@DBABDDCCBDB>B@@CD PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1205:21261:39510 16 chrM 1 70 6S88M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCAT ADCCC>@:DCCCDDCC@:>?89DDDDEC@?>BC>B?@BDDEEFFDEHHGGHHJGGGGJIJJJJJJJJJIJIJJJIIJJJJJHGHHHFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:88
HWI-ST330:434:C50N2ACXX:8:1205:15780:40633 16 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA BCDDDEEDEDEDDDDDDBDDDDDDCEDDDDDDDDFDDB@EEEEEFFEBFECGGHHJJJIIIIHJIJJJJJJIJJJIJJJJJJIIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1205:18807:100182 16 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCNNTTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA DDDDDDEEDDDDDDDDEDB?<<2+##CCB@DC>BBBBDDDDDDDCEEEEEEFFFFFHHHHHHJJJJJJJJJJJJJJJJJJJIJJJJJHHHHGFFFFFCCC PG:Z:novoalign AS:i:82 UQ:i:82 NM:i:2 MD:Z:19T0A74
HWI-ST330:434:C50N2ACXX:8:1206:17354:22801 0 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA @@=DDFFDAFFDHEBFHHCHAC@GF@+CC?EA???GGIICGC:BDHGHIBFEDFGGGEEGEHIHACHAD@@;@CD6>6>>AC;;>5=??C?BB@5>9<>> PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1207:7905:23553 16 chrM 1 44 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA A@CDA@>C?B<BCC>:3A>A>CACCACC>AB>BC;@CAA8(?55?=A=CBEDDECEEEGHHHH@CF@>GF=@GIHGEIHCGIGIIIIHHDFFADDDD?@@ PG:Z:novoalign AS:i:137 UQ:i:137 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1207:18909:78309 0 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CCCFFFFFHHHHHJJJJJJJJJJJJHIJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHHFBEFFFCEDDBDDDDDDD9<@BDEDDDDDBD PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1209:2148:6778 0 chrM 1 70 7S93M * 0 0 CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGA @CCFDFFFHHHHDIJJCFHCGIJIJIJJJJIJJIJJJJJJIJIBHHIJIJGIJJJJJJJJIIIGJHGHEBDFFECA:B59<BDDDDDDDDDDDDDDDDBB PG:Z:novoalign AS:i:82 UQ:i:82 NM:i:0 MD:Z:93
HWI-ST330:434:C50N2ACXX:8:1209:7565:31630 0 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGTGGGTGTGCACGCGATAGCATTG CCCFFFFFHHHHHJJJJJJHIJIJJJJJJJJJJJIJJJJJJJJJJJJJJIIJJJJJJJIJJJJHEHHHHFFFEDDAECDDD@DDDDDDDDDDDDDDDEDD PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:1 MD:Z:67G22
HWI-ST330:434:C50N2ACXX:8:1210:16860:3855 16 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC CDDEEECDCDC@DDCDDDDDDDEEDDCDCDDDDEDD@?EEEEEEEDBFEEHEEHJJJJJIJIJJJIJJJJJJJJJJJIJJJJJIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:78
HWI-ST330:434:C50N2ACXX:8:1210:1998:27127 16 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG DCEEECCDEDDDCADDDDDDDEDC>CDDDDDDEDB?>DEEEEEEB?E@;FHHEJJJJJGHDIIJGGJJIGIJJJJJJJJJJJJJJJJHHHHHFFFFFBCC PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1210:7189:49792 0 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC @CCFFFFFHGFHFGIJIIJGIGIHCHGGJIGJJJIJJIGJJIJJJJJIIIJJJIIJJJ>GHGHGGHHH;@BDFFFABCDDDDDCB55>BDDDDDDDBBDD PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1211:15150:21241 0 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CCCFFFFFHHHHHJJJJJJJJJJJJHIJJIJJJJJJJJJIJJJJJJJJJJJJJJJIJJJJJJHHHGHHHFBEFFFCEDDDDDDDDDD<BBDDEDDDDDDD PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1211:9158:26329 0 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC @@CFFFFFHHHHHJJJIIJIJJJIJJJJJJJFGIJJJIJGJJJJJJJIIJJJIJJJJJFIGHIGHEHHFFFFFFFECCEEFEEDDDDDDDDDD9>BBDED PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:78
HWI-ST330:434:C50N2ACXX:8:1211:18208:40215 0 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC CCCFFFFFHHHHFIJJJJGIJJJCGIIJGJJIJJGJJJJJJGJJJJJ?FHIGEIJJJJJJJJEHEFHHBDFFFFFDDDDBDCDDB9<<<CCCBDDDDDDD PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1211:5064:45314 0 chrM 1 49 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG @@@FFFFFBHFHH?FHIIIIFIIGHHGGII@GHHIIGIIIIIIDHHGIDGGBBBGHGIIHIIHHHHGEE.7;?DFCC==A;;??@<099<@DC?BB59BB PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1211:16473:79186 0 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG CCCFFFFFHHHHHJIJJDHJJJJJJJGGGIEGIIJIJIJJJJJJJJGIJJJJIIJIAGHGCGHHHDECCEFBCBDDCEEEDEBBDDDCABB9)50?@DDD PG:Z:novoalign AS:i:167 UQ:i:167 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1211:6429:88058 0 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG CCCFFFFFHHHFHJJJIJIHGIJJJJGIJIGIJJJJJIGGI@GHGDGHIJJIJJJIJIHIJJJH=AEHFFEFBADCEDDBD5<@@CEDDDDDDDDDDEDD PG:Z:novoalign AS:i:100 UQ:i:100 NM:i:0 MD:Z:90
HWI-ST330:434:C50N2ACXX:8:1212:8168:30628 16 chrM 1 8 25S67M * 0 0 TCCCATTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACAACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGG ??C@@>>-3:>>5-.@@CA?;??7==777.=7);@EC4=;8GCFCB*?8F<@??8?)D?3?*?)::*1ACA<<EC?<AE<C:F<A=17=@=< PG:Z:novoalign AS:i:226 UQ:i:226 NM:i:1 MD:Z:26C40
HWI-ST330:434:C50N2ACXX:8:1212:14322:33250 0 chrM 1 38 17S83M * 0 0 AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGAT CCCFFFFFHHHHHJJJJJJJJJJJJJEHIIJJJJJJJJJJJJJJIJIJJJJJJJJJJJJJJJHHHHHHFFFDEFEEEECDDDDDDDDD5<BDDEDDDDDD PG:Z:novoalign AS:i:142 UQ:i:142 NM:i:0 MD:Z:83
HWI-ST330:434:C50N2ACXX:8:1213:18851:6267 16 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA BDDDDEEEDCDDDDDDDCBBCDDDEEDCBCDC@DB@ADDDDDDB?EEEEECFFFFFHHHHHHJJIJIJJJJJHIHGCJJJJIJJJJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:70 UQ:i:70 NM:i:0 MD:Z:95
HWI-ST330:434:C50N2ACXX:8:1213:12116:75590 0 chrM 1 70 79M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG ?@@D<D?2+22<CB?EFDFGFE>D><E3CG+@*1)??68B<BD@DBBE@DEFIFED@@FGCEEAD;=)'35)558:>A? PG:Z:novoalign AS:i:5 UQ:i:5 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1213:14888:76659 0 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CCCFFFFFHHHHHIJJGHIIIJJJJHHIIJIJJJJJJIJJJIIIJJJIJJIJIJJFIJIJIJJIGJJJJJEHFHHEHFDFDDEDDDD9@BBCDDBDDBBD PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:1 MD:Z:33G50
HWI-ST330:434:C50N2ACXX:8:1213:1817:84430 0 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGTGTGTGCAC @@@FDDADHFFHHJEGHIIGGIJJJGIJGHB3CBGIJGHGGGIGIGI>GHJJJCGDHGHGGIJBECEHFEFECDFCCDA>@DDBABDDDD@;@@B<?CDC PG:Z:novoalign AS:i:201 UQ:i:201 NM:i:1 MD:Z:69G8
HWI-ST330:434:C50N2ACXX:8:1214:17315:65671 0 chrM 1 4 23S65M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCT ???DADDD?2=CDBB:AAFAEEFFII9@E>E@ECDB>DD>BDDD*?90?DEDIADCD05;/@AAADD>DADDD3?(....6;>>???? PG:Z:novoalign AS:i:180 UQ:i:180 NM:i:0 MD:Z:65 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1214:13027:92168 0 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA CCCFFFFFHHHHHJHHIJGIIJJJJIJIJJJJJJIJIJIJJIJJJIJJJJJJJJJIIJJ@GHGGJIH=CFEFDDB>>@<@DDDDDDDBBDDDECDC@DDD PG:Z:novoalign AS:i:70 UQ:i:70 NM:i:0 MD:Z:95
HWI-ST330:434:C50N2ACXX:8:1215:4873:34750 0 chrM 1 32 18S82M * 0 0 AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGA @@CFDADAFFFHHIEEGI<DEHGIGII9?EGGIDHIGIIGIIIGHIIIID@;D@@';(7=@CECE@CADDECD@3(;C>AB@B??CB?0595@@::@BDD PG:Z:novoalign AS:i:151 UQ:i:151 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1215:3505:97966 0 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG @@@DDFDBFFDBDHHIJGH:CFHIIEFHHGGJJJIJIGJJIIGJJG?@AFGIDE@FEH7CAEEE=ACAE@DE6?C=;=@BB09<@CCABBBDB>BCD>AC PG:Z:novoalign AS:i:100 UQ:i:100 NM:i:0 MD:Z:90
HWI-ST330:434:C50N2ACXX:8:1216:12461:8951 16 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG EEEDDDDDC@@<C?89DDCEDCCDC@@4<<DBDDDCDBDDDDDCEDDDEDEEEEFFFHHGIHEIGGHJIHEGGGIHFJIIIJJJJJJGHHDHFFDFFC@@ PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1216:21384:32351 0 chrM 1 53 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC @@<DADD?FFDDAGIICGGGHCGD>H>HGHGHGEIHAGGFHIIIIGI2?6AA8@FGG;=DH<AA=?CEB>BC>@D8=AB@?38?9059@@DA@9&0955@ PG:Z:novoalign AS:i:126 UQ:i:126 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1216:14280:34250 16 chrM 1 70 7S92M * 0 0 CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCG <<2@CCCDC@@@<CCCAC@:1++(:@CDCC?>?>5@<@=6?;>C??9B@BC>A=7=?)@D;=@;45<H?IHFIGEGIIHGAF@CF?2?>AHDDADD@@@ PG:Z:novoalign AS:i:84 UQ:i:84 NM:i:0 MD:Z:92
HWI-ST330:434:C50N2ACXX:8:1301:20249:51368 0 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC CCCFFFFDHDFDHJJJJJJIJJJHHIIJJJJJJJJJIIJJBGIJJJJJJIJJGIIIJJIIIIHH>AHFBDFFFFFA?BDDDCDBD9@BDDEDDDDDDBDC PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1301:14019:55188 16 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG DDDEECACDDCDDDBBDDDDDEDC@CDDDDDDDDBBCDECEEEEB@FC?FHHEJJJJJJIJJJJJJJJJIJJJJJIIJJJJJJIJJJHHHHHFFFFFBCC PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1301:10107:60214 16 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG CDDDCDDCDDDEDB?9DDDEDC>@CBA>BBBBDDDDDDDDDDDDDDDDDDEEECFCFHHFIJGIGJJJJJJIIJIIHJJIJJJJJJIHGHHC1FFFFCCC PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1301:19180:97886 0 chrM 1 70 2S98M * 0 0 TGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGC CCCFFFFFHHGDHIIJIJJIJIJIIJJJJJJJJJJJJIIGIJJIJJJJIJIJJIJI@GHJJJJJGHHHGFFCD@BB@DDDDDDBBDDCCDCCDDDDDDDD PG:Z:novoalign AS:i:52 UQ:i:52 NM:i:0 MD:Z:98
HWI-ST330:434:C50N2ACXX:8:1302:16664:44595 0 chrM 1 26 19S81M * 0 0 TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCG CCCFFDFFHHHHHJJIJJJJJJIJJJJIIIJIJIIJIJJGGIIGDHIJJIIJEGGHEIJGJIHGEEFCFFFFFBC@DCECB?@DDCDDBD9>@@CEDDDD PG:Z:novoalign AS:i:154 UQ:i:154 NM:i:0 MD:Z:81
Raw
with samblaster
-system-specific-4.1$ samtools view -h Control_Input_2_GAGTGG_L008_R1_001.trimmed.sorted.bam | samblaster | head -n 100
samblaster: Version 0.1.17
samblaster: Inputting from stdin
samblaster: Outputting to stdout
samblaster: Loaded 25 header sequence entries.
@HD VN:1.0 SO:coordinate
@PG ID:novoalign PN:novoalign VN:V3.02.00 CL:novoalign -c 7 -d /home/mirrors/igenome/Homo_sapiens/UCSC/hg19/Sequence/NovoalignIndex/WholeGenomeFasta.nvi -f /home/groups/hpcbio/projects/longley/2014-08-04/results/2014-08-06-alignment/Control_Input_2_GAGTGG_L008_R1_001.trimmed.fastq.gz -l 15 -r RANDOM -o SAM
@SQ SN:chrM LN:16571 AS:WholeGenomeFasta.nvi
@SQ SN:chr1 LN:249250621 AS:WholeGenomeFasta.nvi
@SQ SN:chr2 LN:243199373 AS:WholeGenomeFasta.nvi
@SQ SN:chr3 LN:198022430 AS:WholeGenomeFasta.nvi
@SQ SN:chr4 LN:191154276 AS:WholeGenomeFasta.nvi
@SQ SN:chr5 LN:180915260 AS:WholeGenomeFasta.nvi
@SQ SN:chr6 LN:171115067 AS:WholeGenomeFasta.nvi
@SQ SN:chr7 LN:159138663 AS:WholeGenomeFasta.nvi
@SQ SN:chr8 LN:146364022 AS:WholeGenomeFasta.nvi
@SQ SN:chr9 LN:141213431 AS:WholeGenomeFasta.nvi
@SQ SN:chr10 LN:135534747 AS:WholeGenomeFasta.nvi
@SQ SN:chr11 LN:135006516 AS:WholeGenomeFasta.nvi
@SQ SN:chr12 LN:133851895 AS:WholeGenomeFasta.nvi
@SQ SN:chr13 LN:115169878 AS:WholeGenomeFasta.nvi
@SQ SN:chr14 LN:107349540 AS:WholeGenomeFasta.nvi
@SQ SN:chr15 LN:102531392 AS:WholeGenomeFasta.nvi
@SQ SN:chr16 LN:90354753 AS:WholeGenomeFasta.nvi
@SQ SN:chr17 LN:81195210 AS:WholeGenomeFasta.nvi
@SQ SN:chr18 LN:78077248 AS:WholeGenomeFasta.nvi
@SQ SN:chr19 LN:59128983 AS:WholeGenomeFasta.nvi
@SQ SN:chr20 LN:63025520 AS:WholeGenomeFasta.nvi
@SQ SN:chr21 LN:48129895 AS:WholeGenomeFasta.nvi
@SQ SN:chr22 LN:51304566 AS:WholeGenomeFasta.nvi
@SQ SN:chrX LN:155270560 AS:WholeGenomeFasta.nvi
@SQ SN:chrY LN:59373566 AS:WholeGenomeFasta.nvi
HWI-ST330:434:C50N2ACXX:8:1101:1454:8597 0 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA @@@DADBAFFHHGIA@A<AHFE@EEGGHEEHIEHGIGHGHA0@FGHII@CFHGCD;CDGGIFIIE@<=1;;>>ADB09BDCD@?BBDB9>CADD>CB<BD PG:Z:novoalign AS:i:70 UQ:i:70 NM:i:0 MD:Z:95
HWI-ST330:434:C50N2ACXX:8:1101:7050:47300 16 chrM 1 51 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC A@>:CCCA?BC@444:::4?DDCC@>CB<:@ADDDC><:7AAB<BA>CCBFDEFEAAEIGHHD=DGIGCIJIGHEFDEBHEDIJIIGBHGHHFFDFFCCC PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1101:10175:66904 0 chrM 1 70 2S98M * 0 0 TGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCTAGACGC CCCFFFFFHHHFHIJJJJJJJJJJJJJJJIJJJJJJJJJJJJJJJJJJJJJJJJJJFHJJJJJJJJJJIJHFD>@B@DEDDDDDDDDDDEDDDDDDDDDD PG:Z:novoalign AS:i:112 UQ:i:112 NM:i:2 MD:Z:33G57G6
HWI-ST330:434:C50N2ACXX:8:1101:4571:71162 0 chrM 1 53 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC @@@DDDDA<FDAFIIFIIFIEBF+ACHB?EGFGFIFE>G9BGFEIEFIEFIFFAAFD@@=@D?7A=A>(;?BDDD>CCB?=@BBBBB@?@>@?>>B>>9> PG:Z:novoalign AS:i:125 UQ:i:125 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1101:6927:74501 0 chrM 1 37 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA @C@FDDDFBDFH<@GBGGIEHGGGGHGIEHGHGHIIGCHGGEGB@FHGIGHGHGGIGGEGICHAHF@HEB;;@@C>3=A@A?C?BBB5>>BCAC@B@>BB PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1102:15629:92216 0 chrM 1 70 3S97M * 0 0 ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACG CCCFFFFFHHHHBFGGIJJJJJJIJJIJJJIIJJJIIJJGIIIJJJJIIJFJJJJJJHIIIEIIIIHHHHFDDD&0<@CDDDDBDDDBDC<ACCDD>>>B PG:Z:novoalign AS:i:60 UQ:i:60 NM:i:0 MD:Z:97
HWI-ST330:434:C50N2ACXX:8:1103:20430:15560 0 chrM 1 70 1S99M * 0 0 GGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGGGTGCACGCGATAGCATTGCGAGACGCT ?@@DDDDDH=F?DHBH?GCCEGHH>HEEBEHGHIEHEDD:GGBG@GHCD@EDFF@F>@@@FHB=;??9ADC9&0552>>?59&500@@>C:35<5><859 PG:Z:novoalign AS:i:58 UQ:i:58 NM:i:1 MD:Z:71T27
HWI-ST330:434:C50N2ACXX:8:1104:4867:14179 0 chrM 1 49 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG CCCFFFFFHHHHHJJJJJJJJJJJHIJJJJJJJJJJJJJJJJJJJJJJJJIJGIJJIJJJIIHHHHGHHBDFFFFEDDDDDDDDDD5@9@DEDDDDDDDD PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1107:15210:47475 16 chrM 1 70 6S94M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAG <DDDCC>EDCCDDDDDDEDBBADDDEDDC?>BC@DBB?DDDDDDDCEEEEFFFFFFHFHHHJIHHHIJJGJIGJJJJIIJIJIGIJJHHGHHFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:94
HWI-ST330:434:C50N2ACXX:8:1107:14783:91196 1040 chrM 1 70 6S94M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAG <DDDDDEEEDDDDDDDDEDB@CDCCDDDC@>BA>DBBADBCDDDDEEDEDFFFFFFHHHHHEJJJJIIJIJJGIJIJJJJJIIIJJJHHHHGFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:94
HWI-ST330:434:C50N2ACXX:8:1108:11963:23338 1040 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC CDCDC??B<?CCA@A>@ACDCACC>CC??:DDC@>>A?@BA>?;BCCA@>CEECC?HAG@HECDGIHHCFAF@:HEFB<GHEIGIEGDHFDDDBDDF@@C PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1108:12666:26284 0 chrM 1 5 22S67M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGG ==?=DDADDD:4<C:<A<AF;<CDFEEEE<?*?CB99?DC?B3:9DDEBEDD?DDAAAAD<CE7;AA;AADDA@AA@66(6>;85==?? PG:Z:novoalign AS:i:174 UQ:i:174 NM:i:0 MD:Z:67 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1108:6950:92099 0 chrM 1 37 17S82M1S * 0 0 AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGAG CCCFFFDFGHHHHJIJIJIJIJIJIJFHIIJJJJJJJJJJJJJJJJFIIJJIHIIJFGIJJIHHGEHHFFF;@DE>CEC?BDDDDDDD5<<?CEDDDD<@ PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1109:15878:2540 16 chrM 1 70 9S91M * 0 0 ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGC C?BADDDDDEEEDDDDDDDDEDDBDBDDDDCC?>DCCBDB?@DEEEDDEFFFHHHHGHJJJJJJJJJIGIIJJIJIGJJJGHBGHHIGHHHHFFFFFCCC PG:Z:novoalign AS:i:94 UQ:i:94 NM:i:0 MD:Z:91
HWI-ST330:434:C50N2ACXX:8:1109:18041:72542 0 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG @@@DDDFFGDHFDGIIIGDGGJBGGIGHIG9CFHDFCHHIDGHIJIGBDHIIIGIFEHI>AEDCEHHHFBEC?BDA;CEFEEBBDDDCC::@5<9@CCDD PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1110:8402:1988 16 chrM 1 44 16S82M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGA DEDEDDDDDDBDDDDDEEEDDDDDCDDEDD@CDDDEECCBADCADDDADFFFFHHHHJJIJJJJIJJJJJGJJJJJJJIJJJJJJIJHHGHHDFFFD= PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1110:18908:5612 16 chrM 1 70 11S89M * 0 0 ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATT CDDDDDDCDDDEEEDDDDDDDCBAA<9BCDDDCDB>DCCB?DBDDEEDFFEFHHHHHHJJJJJJJJJIHJJIJJJIJJJJIJJJJJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:106 UQ:i:106 NM:i:0 MD:Z:89
HWI-ST330:434:C50N2ACXX:8:1110:11611:66991 16 chrM 1 70 8S92M * 0 0 TCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCG CBADDDDDDEEDDDDDDDDEDB??BDDEECCBADA>DBBADDDECDDCFFFDFFEHEHFJJJJIJJJJIIIJJJJJJJJJJJJIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:88 UQ:i:88 NM:i:0 MD:Z:92
HWI-ST330:434:C50N2ACXX:8:1111:19544:79011 0 chrM 1 70 4S96M * 0 0 GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC @??;ADDD?CFA=<CDFCCEHGHGEGHIHIIIHGGGHB@D)8@BFGHI>FBGFHEFCD6@CED@@G?AHEEEDCB39<<CDCBBBBBBBCCDCCCBBBBB PG:Z:novoalign AS:i:65 UQ:i:65 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1112:8119:19174 1024 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC CCCFFFFFHHHHHJJJIIJIJJJJJJJIJJJGHIIJIJJJJJJJJJIJJJJJJJJJGHGIJJIJJJJJJJJHHHHCEEFFFFFDDDDDDDDDD>BBBDED PG:Z:novoalign AS:i:202 UQ:i:202 NM:i:1 MD:Z:33G44
HWI-ST330:434:C50N2ACXX:8:1113:1327:15419 0 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG ?:=DD?DDBFFDFEI@FG=<<ACHHEE@FFFIIDFBB?FF>BBG@FFI>BAC=@FIEE>@DEEE=CE;>;;BB?@=;=BBBB@<?BDABB9>@BB>BEBB PG:Z:novoalign AS:i:100 UQ:i:100 NM:i:0 MD:Z:90
HWI-ST330:434:C50N2ACXX:8:1113:2480:74049 1024 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CC@FFFADFHGHFIBAGHHGGCHHIEHIIIIIHICF@GGCGIC<GG9?BGG@AE8FEDDEA4).7AEHFF;?B:A>><6,;??CDD@&05?CA>?@>>B< PG:Z:novoalign AS:i:139 UQ:i:139 NM:i:0 MD:Z:84
4
HWI-ST330:434:C50N2ACXX:8:1114:5422:16432 0 chrM 1 24 19S81M * 0 0 TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGGGTGCACGCG @@@DD?DA?BFDDGAFBHH<??FG;9EDGHB<EFCFFGHGHAHGA<<DGHIG;6:/;ACCAHHA>CAHC?BEE;@A>>AC;5;?=??/25&5<@B4A<9< PG:Z:novoalign AS:i:164 UQ:i:164 NM:i:1 MD:Z:71T9
HWI-ST330:434:C50N2ACXX:8:1114:12343:64992 1024 chrM 1 45 15S83M2S * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGAGATCG ?;7?;DDDDDADCEEEE4?:<ADEE9A<E<4CE;CC*:*?9?*:?9?DDDD0-@AD7AC77=??C33;>;3?AD;@86;=;9=?=>&0)2>A:>&050>? PG:Z:novoalign AS:i:176 UQ:i:176 NM:i:1 MD:Z:79C3
HWI-ST330:434:C50N2ACXX:8:1115:5883:14312 1024 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG @@@DFFFDHDFDDFFHIIEE=<E<CGGGIIECHGGHCGGGI@DHCGHIIIII@FH@@FEGGIFFFGFHFFFECEF=;@CCDEB??B@CCB:<09<?C>C< PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1115:7853:81846 16 chrM 1 70 96M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC BBA:43:::4:4+(++8B>:44+84<>3BBB?88,(-(=B@CC@DB>D@B?7=3C>B<BAFFIFF?@D?D8G@F@?)FF:BIFFFDF?@??AD@@? PG:Z:novoalign AS:i:4 UQ:i:4 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1201:4192:2192 1040 chrM 1 70 11S89M * 0 0 ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCAATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATT BC?9@ADDCDCDDC>4DDDDDC@AA?9BDCC@A:?:?>>DB>;DDEEFFFFEHCGHHHGIHC@GJJJIGJIGGJHFJIGFIEJJJIGFHHHHFDFFFCCC PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:1 MD:Z:43C45
HWI-ST330:434:C50N2ACXX:8:1201:13214:10220 16 chrM 1 47 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG :AD@@CAC?<@>CCDDCCDDCDDDCCDC?8,@CCCDC@85B>5?=?;CBEDDBEAE>HEAIEDIIJJJIHAIGGHHHEJJJIIJJJJHHHFFFDDFFC@C PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1201:10956:28155 16 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG ::::@AA>:3>DC8@?DDDDDA>2:?:4?<9?B@CAABDDBCB@>@C@>C@CECB>BHB<GGFFBJIGF?BEF?BFEGJJJJGIGGGFAAABFFFFFCCC PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1201:1702:33608 1024 chrM 1 70 3S97M * 0 0 ATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACG @@@DFFFFFHHHCEGIGJGIJJGGIIECHI>;EEHHGGFIGHGICGHGGGIBGIIJJ=@FEGBGGHCHFFFFDD59<?CCCD>B<B@<A>CCDDDDD>B@ PG:Z:novoalign AS:i:58 UQ:i:58 NM:i:0 MD:Z:97
5
HWI-ST330:434:C50N2ACXX:8:1201:3679:37230 1024 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG CCCFFFFFGGDHHGIGIICEHIIIIGIJJJCGHGGIIHIIJIIIIJJIJIJJJJGHBHHJJIIHHHHHDBDFDFBAACDEEEDDDDDDDBBD&5&8@DDD PG:Z:novoalign AS:i:170 UQ:i:170 NM:i:0 MD:Z:79
5
HWI-ST330:434:C50N2ACXX:8:1202:11253:16296 0 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG CCCFFFFFHFHHHJJJJJJJJJJIJJJJJJJJJJJJIIJJJJJJJJJJJJJJJJGIJJJJJJHIJHHHHDD;@BBCDDDDDDDDDCDEDCDDDDDDDDDD PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
HWI-ST330:434:C50N2ACXX:8:1202:16782:30526 1024 chrM 1 70 100M * 0 0 GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTG @CCFFFFFFCDFDIIJJJJJIJJIGIIJJIJIGGIIJJJJJJJJJGGGIIJIIJFGGIJIEHHIJHHHGDD9@5?:>CDBDB<BBDDECCDDBBBB?B<@ PG:Z:novoalign AS:i:0 UQ:i:0 NM:i:0 MD:Z:100
:79
5
HWI-ST330:434:C50N2ACXX:8:1203:16581:7133 0 chrM 1 32 18S82M * 0 0 AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGA CCCFFDEDHGHHHJJJBHHIEFEGHIJFFH9FHIGIGHHIGIIIIJJJJCHIIIIHJJJGIJEEHHHHFFFFBFDCDDD@;?ADCDDDD5@5?CDDD>B> PG:Z:novoalign AS:i:148 UQ:i:148 NM:i:0 MD:Z:82
HWI-ST330:434:C50N2ACXX:8:1203:2051:23459 0 chrM 1 70 8S92M * 0 0 TCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCCCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCG @@@FDFFFGHHHHJJGB?EHIIJIIJGEGIFGGEIIJFF>FIFJ@F:FHI@@AGIIGIGIIG=?EHAEFFDCCBCEBDD@B5?CDCBB@>BBDDDEDDDD PG:Z:novoalign AS:i:118 UQ:i:118 NM:i:1 MD:Z:41T50
HWI-ST330:434:C50N2ACXX:8:1203:11529:37247 16 chrM 1 38 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA C@CA>BCCCAA?CCCCED@>434:>CCDCA<,BCCDACC<5=;5B?A=A?C;>EA>?@DECIIIIHEEIGFBGD7?ADB7:HIHIIG>FDFD?DDDD@@@ PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1204:16260:97414 1024 chrM 1 70 4S96M * 0 0 GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGAC ?=?BDBADFBFA32?AE>:CBCFBB@HB9CHF@DGFIIF6?F0?F<8;/BC==4CFAFFGIFEEEBCEE;7)9>@-55?A4:?9<@909>>4(>AB3509 PG:Z:novoalign AS:i:66 UQ:i:66 NM:i:0 MD:Z:96
HWI-ST330:434:C50N2ACXX:8:1205:2637:15505 0 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA CCCFFFFDHHHHFIGHGIJJIIIIJIIIJGIIGGHHGIJJJJJIHGGIGCGIJIBGGGGGGGHHHHGDDFCCFDEDCCECED@DBABDDCCBDB>B@@CD PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1205:21261:39510 16 chrM 1 70 6S88M * 0 0 ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCAT ADCCC>@:DCCCDDCC@:>?89DDDDEC@?>BC>B?@BDDEEFFDEHHGGHHJGGGGJIJJJJJJJJJIJIJJJIIJJJJJHGHHHFFFFFCCC PG:Z:novoalign AS:i:76 UQ:i:76 NM:i:0 MD:Z:88
HWI-ST330:434:C50N2ACXX:8:1205:15780:40633 16 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA BCDDDEEDEDEDDDDDDBDDDDDDCEDDDDDDDDFDDB@EEEEEFFEBFECGGHHJJJIIIIHJIJJJJJJIJJJIJJJJJJIIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1205:18807:100182 16 chrM 1 70 5S95M * 0 0 CGATGGATCACAGGTCTATCACCCNNTTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGA DDDDDDEEDDDDDDDDEDB?<<2+##CCB@DC>BBBBDDDDDDDCEEEEEEFFFFFHHHHHHJJJJJJJJJJJJJJJJJJJIJJJJJHHHHGFFFFFCCC PG:Z:novoalign AS:i:82 UQ:i:82 NM:i:2 MD:Z:19T0A74
HWI-ST330:434:C50N2ACXX:8:1206:17354:22801 1024 chrM 1 4 23S77M * 0 0 CCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCA @@=DDFFDAFFDHEBFHHCHAC@GF@+CC?EA???GGIICGC:BDHGHIBFEDFGGGEEGEHIHACHAD@@;@CD6>6>>AC;;>5=??C?BB@5>9<>> PG:Z:novoalign AS:i:178 UQ:i:178 NM:i:0 MD:Z:77 ZS:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1207:7905:23553 1040 chrM 1 44 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA A@CDA@>C?B<BCC>:3A>A>CACCACC>AB>BC;@CAA8(?55?=A=CBEDDECEEEGHHHH@CF@>GF=@GIHGEIHCGIGIIIIHHDFFADDDD?@@ PG:Z:novoalign AS:i:137 UQ:i:137 NM:i:0 MD:Z:84
HWI-ST330:434:C50N2ACXX:8:1207:18909:78309 1024 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CCCFFFFFHHHHHJJJJJJJJJJJJHIJIJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJHHHHHHHFBEFFFCEDDBDDDDDDD9<@BDEDDDDDBD PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
S:Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1209:2148:6778 0 chrM 1 70 7S93M * 0 0 CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGA @CCFDFFFHHHHDIJJCFHCGIJIJIJJJJIJJIJJJJJJIJIBHHIJIJGIJJJJJJJJIIIGJHGHEBDFFECA:B59<BDDDDDDDDDDDDDDDDBB PG:Z:novoalign AS:i:82 UQ:i:82 NM:i:0 MD:Z:93
HWI-ST330:434:C50N2ACXX:8:1209:7565:31630 1024 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGTGGGTGTGCACGCGATAGCATTG CCCFFFFFHHHHHJJJJJJHIJIJJJJJJJJJJJIJJJJJJJJJJJJJJIIJJJJJJJIJJJJHEHHHHFFFEDDAECDDD@DDDDDDDDDDDDDDDEDD PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:1 MD:Z:67G22
Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1210:16860:3855 16 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC CDDEEECDCDC@DDCDDDDDDDEEDDCDCDDDDEDD@?EEEEEEEDBFEEHEEHJJJJJIJIJJJIJJJJJJJJJJJIJJJJJIIJJHHHHHFFFFFCCC PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:78
HWI-ST330:434:C50N2ACXX:8:1210:1998:27127 16 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG DCEEECCDEDDDCADDDDDDDEDC>CDDDDDDEDB?>DEEEEEEB?E@;FHHEJJJJJGHDIIJGGJJIGIJJJJJJJJJJJJJJJJHHHHHFFFFFBCC PG:Z:novoalign AS:i:166 UQ:i:166 NM:i:0 MD:Z:79
HWI-ST330:434:C50N2ACXX:8:1210:7189:49792 1024 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC @CCFFFFFHGFHFGIJIIJGIGIHCHGGJIGJJJIJJIGJJIJJJJJIIIJJJIIJJJ>GHGHGGHHH;@BDFFFABCDDDDDCB55>BDDDDDDDBBDD PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
HWI-ST330:434:C50N2ACXX:8:1211:15150:21241 1024 chrM 1 43 16S84M * 0 0 ATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATA CCCFFFFFHHHHHJJJJJJJJJJJJHIJJIJJJJJJJJJIJJJJJJJJJJJJJJJIJJJJJJHHHGHHHFBEFFFCEDDDDDDDDDD<BBDDEDDDDDDD PG:Z:novoalign AS:i:136 UQ:i:136 NM:i:0 MD:Z:84
2
Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1211:9158:26329 1024 chrM 1 8 22S78M * 0 0 CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCAC @@CFFFFFHHHHHJJJIIJIJJJIJJJJJJJFGIJJJIJGJJJJJJJIIJJJIJJJJJFIGHIGHEHHFFFFFFFECCEEFEEDDDDDDDDDD9>BBDED PG:Z:novoalign AS:i:172 UQ:i:172 NM:i:0 MD:Z:78
HWI-ST330:434:C50N2ACXX:8:1211:18208:40215 1024 chrM 1 55 14S86M * 0 0 AAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGC CCCFFFFFHHHHFIJJJJGIJJJCGIIJGJJIJJGJJJJJJGJJJJJ?FHIGEIJJJJJJJJEHEFHHBDFFFFFDDDDBDCDDB9<<<CCCBDDDDDDD PG:Z:novoalign AS:i:124 UQ:i:124 NM:i:0 MD:Z:86
2
Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1211:5064:45314 1024 chrM 1 49 15S85M * 0 0 TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAG @@@FFFFFBHFHH?FHIIIIFIIGHHGGII@GHHIIGIIIIIIDHHGIDGGBBBGHGIIHIIHHHHGEE.7;?DFCC==A;;??@<099<@DC?BB59BB PG:Z:novoalign AS:i:130 UQ:i:130 NM:i:0 MD:Z:85
HWI-ST330:434:C50N2ACXX:8:1211:16473:79186 1024 chrM 1 14 21S79M * 0 0 CTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACG CCCFFFFFHHHHHJIJJDHJJJJJJJGGGIEGIIJIJIJJJJJJJJGIJJJJIIJIAGHGCGHHHDECCEFBCBDDCEEEDEBBDDDCABB9)50?@DDD PG:Z:novoalign AS:i:167 UQ:i:167 NM:i:0 MD:Z:79
2
Z:R NH:i:2 HI:i:1 IH:i:1
HWI-ST330:434:C50N2ACXX:8:1211:6429:88058 1024 chrM 1 70 10S90M * 0 0 CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTG CCCFFFFFHHHFHJJJIJIHGIJJJJGIJIGIJJJJJIGGI@GHGDGHIJJIJJJIJIHIJJJH=AEHFFEFBADCEDDBD5<@@CEDDDDDDDDDDEDD PG:Z:novoalign AS:i:100 UQ:i:100 NM:i:0 MD:Z:90
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