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package org.hammerlab.guacamole.commands | |
import org.apache.spark.SparkContext | |
import org.bdgenomics.formats.avro.AlignmentRecord | |
import org.hammerlab.guacamole.{ DistributedUtil, SparkCommand, Common } | |
import org.bdgenomics.adam.rdd.ADAMContext._ | |
import org.kohsuke.args4j.Option | |
import org.bdgenomics.adam.rdd.ADAMSaveAnyArgs | |
/** | |
* Created by danvk on 6/25/15. | |
*/ | |
object StructuralVariant { | |
protected class Arguments extends Common.Arguments.Reads with DistributedUtil.Arguments with ADAMSaveAnyArgs { | |
@Option(name = "--filter-contig", usage = "Filter to alignments where either mate is in this contig.") | |
var filterContig: String = "" | |
// These are required by ADAMSaveAnyArgs | |
@Option(name = "--out", required = true, usage = "Location to write the transformed data in ADAM/Parquet format") | |
var outputPath: String = null | |
@Option(required = false, name = "-sort_fastq_output", usage = "Sets whether to sort the FASTQ output, if saving as FASTQ. False by default. Ignored if not saving as FASTQ.") | |
var sortFastqOutput: Boolean = false | |
} | |
object Caller extends SparkCommand[Arguments] { | |
override val name = "structural-variant" | |
override val description = "TBD" | |
def matchesContig(alignment: AlignmentRecord, contig: String) = { | |
alignment.getContig == contig || (alignment.getMateContig != null && alignment.getMateContig.getContigName == contig) | |
} | |
override def run(args: Arguments, sc: SparkContext): Unit = { | |
val filterContig = args.filterContig | |
val alignments = sc.loadAlignments(args.reads) | |
val matchingAlignments = alignments.filter(matchesContig(_, filterContig)) | |
matchingAlignments.persist() | |
println("Found " + matchingAlignments.count() + " alignments with one pair in " + filterContig) | |
matchingAlignments.coalesce(10).adamSAMSave(args.outputPath, asSam = false) | |
} | |
} | |
} |
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