Created
January 15, 2016 17:22
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[ecwheele@tscc-login1 mds_splicing_v4]$ bash process_rnaseq.sh | |
INFO 09:21:27,273 QScriptManager - Compiling 1 QScript | |
INFO 09:21:36,790 QScriptManager - Compilation complete | |
INFO 09:21:36,952 HelpFormatter - ---------------------------------------------------------------------- | |
INFO 09:21:36,953 HelpFormatter - Queue v2.3-1155-g931452b, Compiled 2016/01/11 23:38:39 | |
INFO 09:21:36,953 HelpFormatter - Copyright (c) 2012 The Broad Institute | |
INFO 09:21:36,953 HelpFormatter - For support and documentation go to http://www.broadinstitute.org/gatk | |
INFO 09:21:36,954 HelpFormatter - Program Args: -S /home/ecwheele/gscripts/qscripts/analyze_rna_seq.scala --input manifest.txt --adapter TCGTATGCCGTCTTCTGCTTG --adapter ATCTCGTATGCCGTCTTCTGCTTG --adapter CGACAGGTTCAGAGTTCTACAGTCCGACGATC --adapter GATCGGAAGAGCACACGTCTGAACTCCAGTCAC -qsub -jobQueue home-scrm -log mds_splicing_v4.log --location /oasis/tscc/scratch/ecwheele/mds_splicing_v4/ --strict -keepIntermediates --flipped flip -run | |
INFO 09:21:36,954 HelpFormatter - Date/Time: 2016/01/15 09:21:36 | |
INFO 09:21:36,954 HelpFormatter - ---------------------------------------------------------------------- | |
INFO 09:21:36,954 HelpFormatter - ---------------------------------------------------------------------- | |
INFO 09:21:36,962 QCommandLine - Scripting AnalyzeRNASeq | |
INFO 09:21:37,232 QCommandLine - Done with errors | |
##### ERROR ------------------------------------------------------------------------------------------ | |
##### ERROR stack trace | |
org.broadinstitute.sting.utils.exceptions.UserException$CannotExecuteQScript: Unable to execute QScript: AnalyzeRNASeq.script() threw the following exception: java.lang.NullPointerException | |
at org.broadinstitute.sting.queue.QCommandLine$$anonfun$execute$5.apply(QCommandLine.scala:159) | |
at org.broadinstitute.sting.queue.QCommandLine$$anonfun$execute$5.apply(QCommandLine.scala:147) | |
at scala.collection.Iterator$class.foreach(Iterator.scala:772) | |
at scala.collection.JavaConversions$JIteratorWrapper.foreach(JavaConversions.scala:573) | |
at scala.collection.IterableLike$class.foreach(IterableLike.scala:73) | |
at scala.collection.JavaConversions$JListWrapper.foreach(JavaConversions.scala:615) | |
at org.broadinstitute.sting.queue.QCommandLine.execute(QCommandLine.scala:147) | |
at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:245) | |
at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:152) | |
at org.broadinstitute.sting.queue.QCommandLine$.main(QCommandLine.scala:62) | |
at org.broadinstitute.sting.queue.QCommandLine.main(QCommandLine.scala) | |
Caused by: java.lang.NullPointerException | |
at org.broadinstitute.sting.queue.QScript$class.swapExt(QScript.scala:86) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq.swapExt(analyze_rna_seq.scala:14) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq$$anonfun$script$3$$anonfun$apply$1.apply(analyze_rna_seq.scala:340) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq$$anonfun$script$3$$anonfun$apply$1.apply(analyze_rna_seq.scala:319) | |
at scala.collection.LinearSeqOptimized$class.foreach(LinearSeqOptimized.scala:59) | |
at scala.collection.immutable.List.foreach(List.scala:76) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq$$anonfun$script$3.apply(analyze_rna_seq.scala:319) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq$$anonfun$script$3.apply(analyze_rna_seq.scala:315) | |
at scala.collection.immutable.Map$Map1.foreach(Map.scala:119) | |
at org.broadinstitute.sting.queue.qscripts.AnalyzeRNASeq.script(analyze_rna_seq.scala:315) | |
at org.broadinstitute.sting.queue.QCommandLine$$anonfun$execute$5.apply(QCommandLine.scala:156) | |
... 10 more | |
##### ERROR ------------------------------------------------------------------------------------------ | |
##### ERROR A GATK RUNTIME ERROR has occurred (version 2.3-1155-g931452b): | |
##### ERROR | |
##### ERROR Please check the documentation guide to see if this is a known problem | |
##### ERROR If not, please post the error, with stack trace, to the GATK forum | |
##### ERROR Visit our website and forum for extensive documentation and answers to | |
##### ERROR commonly asked questions http://www.broadinstitute.org/gatk | |
##### ERROR | |
##### ERROR MESSAGE: Unable to execute QScript: AnalyzeRNASeq.script() threw the following exception: java.lang.NullPointerException | |
##### ERROR ------------------------------------------------------------------------------------------ | |
INFO 09:21:37,252 QCommandLine - Shutting down jobs. Please wait... |
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