build a seperate python gtk develop environment for windows, this is a open to use environment, no need to install python. run `setup.py build` and copy the build directory to an other windows, you can use python27_console.exe to run the script.

python27.py

#!python
# -*- coding: utf-8 -*-

import code
import os
import sys

if __name__ == "__main__":
    libraries = ["python27.zip", "library.zip"]
    for f in libraries:
        f1 = os.path.abspath(f)
        if os.path.exists(f1):
            sys.path.insert(0, f1)
    sys.path.append(".")
    if len(sys.argv) > 1:
        if os.path.exists(sys.argv[1]):
            sys.argv = sys.argv[1:]
            execfile(sys.argv[0])
        else:
            raise OSError("No such file: %s" % sys.argv[1])
    else:
        code.interact()

setup.py

#!python
import sys
import os
import glob
# from distutils.core import Extension
from cx_Freeze import setup, Executable


consoleBase = None
guiBase = None
if sys.platform == "win32":
    guiBase = "Win32GUI"

includefiles = []


for s in [
    "*.xml",
    "*.ini",
    "*.pyd",
    "*.cfg",
    # "*.doc",
    # "*.docx",
    #"*.mdb",
    "*.json",
    #"*.txt",
    #"*.sql",
     ]:
    for f in glob.glob(s):
        includefiles.append((f, os.path.basename(f)))

glade_root = r'D:\Program Files\Glade Interface Designer Gtk+ 2'

files = ["bin/glade-3.exe", "bin/gspawn-win32-helper.exe",
         "bin/gspawn-win32-helper-console.exe",
         "lib", "share", "glade-3.ico",
         ]

for f in files:
    includefiles.append((os.path.join(glade_root, f), os.path.basename(f)))

gtk_root = "e:/gtk2.24/gtk"

files = ["etc", "lib/gtk-2.0", "lib/gnome-vfs-2.0", "lib/GConf-dbus",
         "lib/orbit-2.0", "share/locale/zh", "share/locale/zh_CN",
         "share/themes", "share/icons"]

for f in files:
    includefiles.append((os.path.join(gtk_root, f), f))

py_modules = [
    "glib", "gtk", "gobject", "gio", "pango", "ctypes",
    "types", "win32con", "win32process", "win32com.shell.shell",
    "threading", "re", "wmi", "socket", "select", "traceback",
    "Queue", "platform", "StringIO", "pythoncom", "pycurl2", "urllib",
    "urllib2", "hashlib", "Crypto.Cipher.AES", "Crypto.Cipher.ARC4",
    "Crypto.Random", "lxml.etree", "lxml._elementpath", "gtk.keysyms",
    "time", "pymysql", "adodbapi", "ConfigParser", "json",
    "win32net", "win32netcon", "win32wnet", "pywintypes", "telnetlib",
    "httplib2", "urlparse", "random", "pygtk", "win32file", "nfc.ndef",
    "smartcard.System", "smartcard.util", "xml.etree.ElementTree",
    "win32event", "win32com.shell.shellcon", "logging", "logging.handlers"

]
setup(
      version="0.0.1",
      options={"build_exe": {"include_files": includefiles,
                             "excludes": ["Tkinter"],
                             "includes": py_modules,
                             "include_msvcr": True
                             }},
      executables=[
            Executable("python27.py",
                       base=consoleBase,
                       targetName="python27_console.exe",
                       icon="D:\Python27\DLLs\py.ico"
                       ),
            Executable("python27.py",
                       base=guiBase,
                       targetName="python27_gui.exe",
                       icon="D:\Python27\DLLs\py.ico",
                       ),

            ]
      )