Created
August 9, 2019 11:37
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Macro running skeleton analysis in batch mode
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#@ File (label = "Input directory", style = "directory") input | |
#@ File (label = "Output directory", style = "directory") output | |
#@ String (label = "File suffix", value = ".jpg") suffix | |
processFolder(input); | |
function processFolder(input) { | |
list = getFileList(input); | |
list = Array.sort(list); | |
for (i = 0; i < list.length; i++) { | |
if(File.isDirectory(input + File.separator + list[i])) | |
processFolder(input + File.separator + list[i]); | |
if(endsWith(list[i], suffix)) { | |
setBatchMode(true); | |
processFile(input, output, list[i]); | |
setBatchMode(false); | |
} | |
} | |
} | |
function processFile(input, output, file) { | |
open(input + File.separator + file); | |
run("8-bit"); | |
run("Duplicate...", "title=duplicate"); | |
selectWindow("duplicate"); | |
run("Gaussian Blur...", "sigma=5"); | |
run("Auto Local Threshold", "method=Mean radius=150 parameter_1=0 parameter_2=0"); | |
run("Fill Holes"); | |
run("Skeletonize (2D/3D)"); | |
selectWindow("duplicate"); | |
run("Divide...", "value=255.000"); | |
imageCalculator("Multiply create", file,"duplicate"); | |
run("Analyze Skeleton (2D/3D)", "prune=none show"); | |
close("Tagged skeleton"); | |
selectWindow("Branch information"); | |
saveAs("Results", output + File.separator + file + ".csv"); | |
close(file + ".csv"); | |
close("Result of " + file); | |
close("duplicate"); | |
close(file); | |
} |
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