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Very small sequence collection for use in QIIME tests (under development).
Raw
README.md

Tiny test data sequence collection for use with QIIME.

This is being compiled to address #582.

Using this data

You can see the output of a few commands by downloading this data and running the cmds.sh shell script from inside the unzipped directory.

Desired properties of the test data

[X] Samples form a meaningful pattern in unifrac PCoA space (e.g., two clusters of two samples each).

[X] Sample categories differ in alpha diversity.

[X] A small reference collection is included (~3 sequences, which are greater than 3x longer than the input reads).

[X] When clustered against this reference at 97% identity, some of the reads should hit the reference and some should not. At least one of the input reads should be <60% similar to any of the reference sequences.

[X - see samples f2,f3] Two of the samples should contain identical sequences.

[X - see sample not16S.1] One sample should contain seqs that are not 16s and all drop out.

[X - see sample f4] One sample should have seqs that are all identical.

Compilation notes

These are not fully exhaustive because this was some exploratory analysis to compile a good data set. Saving these here for future reference.

Start with sequences from moving pictures tutorial dataset extract_seqs_by_sample_id.py -i ../slout/seqs.fna -o in.fna -s L1S3,L1S2,L2S97,L2S97,L2S98,L5S97,L5S98

pick_otus.py -i in.fna -o ucr_97 -m uclust_ref -r ~/data/gg_12_10_otus/rep_set/97_otus.fasta

single_rarefaction.py -d 69 -i otu_table.biom -o otu_table_even69.biom 

beta_diversity.py -i otu_table_even69.biom -o dms/ -m bray_curtis

principal_coordinates.py -i dms/bray_curtis_otu_table_even69.txt -o dms/pc.tct

make_3d_plots.py -i dms/pc.tct -o dms/plots/ -m /Users/caporaso/outbox/test_set/map.txt

We see visual separation of SampleType in alpha rarefaction, but not e.g by PrimerPlate (negative control)...

alpha_rarefaction.py -i otu_table.biom -e 30 -o arare_30/ -m /Users/caporaso/outbox/test_set/map.txt -t pynast_aligned/candidate_rep_set_aligned.pfiltered.tre

Results are not significant, but likely bc of low sample size.

compare_alpha_diversity.py -i arare_30/alpha_div_collated/PD_whole_tree.txt -d 30 -o aldiv_t.txt -m /Users/caporaso/outbox/test_set/map.txt -c SampleType -p none -t parametric

Computed beta diversity for all rarefied OTU tables

beta_diversity.py -i arare_30/rarefaction/ -o arare_30/dms/ -t pynast_aligned/candidate_rep_set_aligned.pfiltered.tre

Then did all-within v all-between SampleType comparisons with make_distance_boxplots.py (representative command below) and sorted results by t-score. identified rarefaction/rarefaction_22_7.biom as a table that gave good clustering in PCoA space and reasonable differences in alpha diversity.

make_distance_boxplots.py -d unweighted_unifrac_rarefaction_22_7.txt -m /Users/caporaso/outbox/test_set/map.txt -f SampleType -o unweighted_unifrac_rarefaction_22_7.txt_boxplots/ -n 1000

alpha_diversity.py -i rarefaction/rarefaction_22_7.biom -o adiv_22.txt -t /Users/caporaso/outbox/test_set/pynast_aligned/candidate_rep_set_aligned.pfiltered.tre

Converted that biom to txt, and used that to inform the sequence collection for the test set.

Raw
cmds.sh
#!/usr/bin/env bash
pick_otus_through_otu_table.py -i seqs.fna -p ucr_params.txt -o ucr/
beta_diversity_through_plots.py -i ucr/otu_table.biom -o ucr/bdiv/ -t ucr/rep_set.tre -m map.txt
alpha_rarefaction.py -i ucr/otu_table.biom -o ucr/arare/ -t ucr/rep_set.tre -m map.txt
summarize_taxa_through_plots.py -i ucr/otu_table.biom -o ucr/taxa_plots/
Raw
map.txt
#SampleID BarcodeSequence LinkerPrimerSequence SampleType year month day subject days_since_epoch Description
f1 ACACTGTTCATG GTGCCAGCMGCCGCGGTAA feces 2008 10 22 1 14174 fecal1
f2 ACCAGACGATGC GTGCCAGCMGCCGCGGTAA feces 2008 10 23 1 14175 fecal2
f3 ACCAGACGATGC GTGCCAGCMGCCGCGGTAA feces 2008 10 23 1 14175 identical sequences to fecal2
f4 ACCAGACGATGC GTGCCAGCMGCCGCGGTAA feces 2008 10 23 1 14175 all sequences identical, map to GG 295053 at 97 percent id
p1 AACGCACGCTAG GTGCCAGCMGCCGCGGTAA L_palm 2008 10 21 1 14173 palm1, contains one randomly generated sequence
p2 ACACTGTTCATG GTGCCAGCMGCCGCGGTAA L_palm 2008 10 22 1 14174 palm2
t1 AGTGAGAGAAGC GTGCCAGCMGCCGCGGTAA Tongue 2008 10 21 1 14173 tongue1, contains one randomly generated sequence
t2 ATACTATTGCGC GTGCCAGCMGCCGCGGTAA Tongue 2008 10 22 1 14174 tongue2
not16S.1 ATACTATTGCGC GTGCCAGCMGCCGCGGTAA Other 2008 10 22 1 14174 randomly generated sequence plus some variants, these should not map to 16S
Raw
ref.fna
>426848
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAATACATGCAAGTCGAGGGGCAGCACTGGTAGCAATACCTGGTGGCGACCGGCGGACGGGTGCGTAACACGTATGCAACCTACCCTGTACAGGGGGATAGCCCGAGGAAATTCGGATTAATACCCCATACGATAAGAATCGGCATCGATTTTTATTGAAAGCTCCGGCGGTACAGGATGGGCATGCGCCCCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCTACGATGGGTAGGGGGCCTGAGAGGGTGATCCCCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTAAGGAATATTGGTCAATGGGCGCAAGCCTGAACCAGCCATGCCGCGTGCAGGAAGACTGCCATTATGGTTGTAAACTGCTTTTATATGGGAAGAAACCTCCGGACGTGTCCGGAGCTGACGGTACCATGTGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCGTGTTAAGTCAGAGGTGAAATTCGGCAGCTCAACTGTCAAATTGCCTTTGATACTGGCACACTTGAATGCGATTGAGGTAGGCGGAATGTGACATGTAGCGGTGAAATGCTTAGACATGTGACAGAACACCGATTGCGAAGGCAGCTTACCAAGTCGTTATTGACGCTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAACTCGACGTTAGCGATACACTGTTAGCGTCCAAGCGAAAGCGTTAAGTTATCCACCTGGGAAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCAGGGCTTAAATGGGGAACGACCTTCTGGGAAACCAGAATTTCTTTTAGACGGTCCTCAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTACTGTTAGTTGCCAGCGGATAATGCCGGGGACTCTAGCGGAACTGCCTGTGCAAACAGAGAGGAAGGTGGGGATGACGTCAAATCATCACGGCCCTTACGTCCTGGGCTACACACGTGCTACAATGGCCGGTACAGAGGGCAGCCACTTCGTGAGAAGGAGCGAATCCTTAAAGCCGGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGTATCAGCCATGACGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGGGAATTGGGAGTACCTAAAGTCGGTAACCGCAAGGAGCCGCCTAAGGTAATACCAGTGACTGGGGCTAAGTCGTAACAAGGTAGCCGTA
>42684
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCAGCACAGAGGAGCTTGCTTCTTGGGTGGCGAGTGGCGAACGGGTGAGTGACGCATCGGAACGTACCGAGTAATGGGGGATAACTGTCCGAAAGGACAGCTAATACCGCATACGCCCTGAGGGGGAAAGCGGGGGATCTTAGGACCTCGCGTTATTCGAGCGGCCGATGTCTGATTAGCTGGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCAGTAGCGGGTCTGAGAGGATGATCCGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGTCTGAAGAAGGCCTTCGGGTTGTAAAGGACTTTTGTCAGGGAAGAAAAGGAACGTGTTAATACCATGTTCTGATGACGGTACCTGAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCGGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCCGAACTGGGTGGCTAGAGTGTGTCAGAGGGGGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAGAGATGTGGAGGAATACCGATGGCGAAGGCAGCCCCCTGGGATAACACTGACGTTCATGCCCGAAAGCGTGGGTAGCAAACAGGGTTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCGATTAGCTGTTGGGGCACTTGATGCCTTAGTAGCGTAGCTAACGCGTGAAATCGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATGTACGGAATCTTCCAGAGACGGAAGGGTGCCTTCGGGAGCCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCACTTGGTTGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGACCAGGGCTTCACACGTCATACAATGGTCGGTACAGAGGGTAGCCAAGCCGCGAGGCGGAGCCAATCCCAGAAAACCGATCGTAGTCCGGATTGCACTCTGCAACTCGAGTGCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGGATACCAGAAGCAGGTAGGCTAACCGCAAGGAGGCCGCTTGCCACGGTATGCTTCATGACTGGGGTGAAGTCGTAACAAGGTAAC
>342684
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCATCGCGGGTAGCAATACCTGGCGGCGACCGGCGGAAGGGTGCGTAACGCGTGAGCGACATACCCGTGACAGGGGGATAACAGATGGAAACGTCTCCTAATACCCCATAAGATCATATATCGCATGGTATGTGATTGAAAGGTGAGAACCGGTCACGGATTGGCTCGCGTCCCATCAGGTAGACGGCGGGGCAGCGGCCCGCCGTGCCGACGACGGGTAGGGGCTCTGAGAGGAGTGACCCCCACAATGGAACTGAGACACGGTCCATACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGCGGGAGGACGGCCCTATGGGTTGTAAACCGCTTTTGAGTGAGAGCAATAAGGTTCACGTGTGGACCGATGAGAGTATCATTCGAATAAGCATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTAGGCGGACATGTAAGTCCGAGGTGAAAGACCGGGGCCCAACCCCGGGGTTGCCTCGGATACTGTGTGTCTGGAGTGGACGTGCCGCCGGGGGAATGAGTGGTGTAGCGGTGAAATGCATAGATGTCACTCAGAACACCGATTGCGAAGGCACCTGGCGAATGTCTTACTGACGCTGAGGCACGAAAGCGTGGGGATCGAACAGGATTAGATACCCTGGTAGTCCACGCAGTAAACGATGATGGCTGTCCGTTCGCTCCGATAGGAGTGAGTAGACAAGCGAAAGCGCTAAGCCATCCACCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGAGGAACATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGGGCTCGAACGGCAGGTGAACGATGCAGAGATGCAAAGGCCCTTCGGGGCGTCTGTCGAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTCAAGTGCCATAACGAGCGCAACCCTTGCCTGCAGTTGCCATCGGGTAAAGCCGGGGACTCTGCAGGGACTGCCACCGCAAGGTGAGAGGAGGGGGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCGACACACGTGTTACAATGGCGGCCACAGCGGGAAGCCACCCAGTGATGGGGCGCGGATCCCAAAAAAGCCGCCTCAGTTCGGATCGGAGTCTGCAACCCGACTCCGTGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCATGGCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGGGAGTCGTGGGCGCCTGAAGGCCGTGACCGCGAGGAGCGGCCTAGGGCGAACGCGGTGACTGGGGCTAAGTCGTAACAAGGTA
>295053
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGCTCCTTCGGGAGTATCTTAGTGGCGAACGGGTGAGTAACGCGTGAGCAACCTGACCTTCACAGGGGGATAACCGCTGGAAACAGCAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAACGGCTCACCAAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGCACACACCGCC
>879972
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGATTGACCGGTGCTTGCACTGGTCAATCTAGTGGCGAACGGGTGAGTAACACGTGGGTAACCTGCCCATCAGAGGGGGATAACATTCGGAAACGGATGCTAAAACCGCATAGGTCTTCGAACCGCATGGTTTGAAGAGGAAAAGAGGCGCAAGCTTCTGCTGATGGATGGACCCGCGGTGTATTAGCTAGTTGGTGGGGTAACGGCTCACCAAGGCGACGATACATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGACCGAGCAACGCCGCGTGAGTGAAGAAGGTTTTCGGATCGTAAAGCTCTGTTGTAAGAGAAGAACGAGTGTGAGAGTGGAAAGTTCACACTGTGACGGTATCTTACCAGAAAGGGACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAGAGGGGAGAGTGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAGGAACACCGGTGGCGAAAGCGGCTCTCTGGCTTGTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTAGACCCTTTCCGGGGTTTAGTGCCGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAGGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGACCGCTCTAGAGATAGAGCTTTCCTTCGGGACAGAGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTGTTAGTTGCCATCATTCAGTTGGGCACTCTAGCGAGACTGCCGGTAATAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGCTGGTACAACGAGTCGCAAGCCGGTGACGGCAAGCTAATCTCTTAAAGCCAGTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATCGCTAGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGCCT
Raw
refseqs_aligned.fna
>426848
------------------------------------------------------------------------------------------------------------AGAGTTT-GA--T-CC-T-G-GCTC-AG-GA-TGAA-C-GC--TAG-C--G-GC-A-GG--C----T-T--AATACA-T-GC-A-AGT-CGA-G-GG----------G-CAGCA---C-----------------------------TG-G-TA----------------------------------------------------GCA-A----------------------------------------------------------------------------------TAC-CT------------------G-GT--G--GC--G--AC-C-GG-C-GG-A--C-------------GGG-TGCGT-A--AC-AC-G-T-A-TG---CAA--C-CT-A--C-C-CTG--TA-C------------------------------------------------------------------A-GG----GGG-AT-AG-CCC-------------------------G-A-G-----------------------GAA-A---TTC-GGA-TTAA-TA---CC-C--C-AT-A----------C--------------------G-A----T-A--A--G-----------------AA---TC-G-----------------------------------------------------------------------------------------------------------------------G-CA-T--------------------------------------------------------------------------------------------------------------------------------------C-G-A--T-T---------------T--T-T-A-T-T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAAA--G-C-T--------------------------------------------------------------------------------------------------------------------------------------CCG------------------------------------------------------------------------------------------------------------------------------------G---C-G--------------G----T-A---C-AG-G---AT---G-G-----G-CAT-GCG--C-CCC--A------TT--A--G-CT-A----G---TTGG-T-G-AG-G-T----AAC-GG-C-T-C-ACCA--A-GG-C-T--A-CG-A------------TGG-G-T------AG-GG-G-G-CCT-G-A-G---A--GG-GT--G-AT-C-CC-CCAC-A-CTGGA--A-C-TG-A-GA-C-AC-G-G-TCCAGA-CTCC-TAC-G--G-G-A-G-GC-A-GC-A-G-TA---AG-G-A-ATA-TTGGT-C-AA-T-GG--GC-GC-A----A-G-CC-T-GA-AC-CA-GCCA-TGCC-G-CG-T---G-C-A--G--GA-A-G--A--C-T-G-CCA----TT-AT---------G-G-T-T-G-T--A---AA-C-TGC--------TT-TT-A-T--A-TGG----GA-A--G---AAACCTC---CGGA----------C--GT-G-----------------T-CCG-GAG-CT-GA-CG-GT-A-C-CA-T-GT-G---------AA-----------TAAGG-ATC-GG-C-TAA---C--T-CCGT--GCCA--G-C---A--GCCG---C-GG--TA-AT--AC---GG-AG-GAT-CCA-A-G-CG-TTAT-C-CGG-AT-TT-A--T-T--GGGT-TTA----AA-GGGT-GC--G-TA-G-G-C-G------------G--C-GT-G-T-T-AA----G-T-C-A---G-AGG-TG-A-AA-TT--CG-GCA-G--------------------------------------------------------------------CT-C-AA-------------------------------------------------------------------------CT-G-T-CA-AA-T----T-G-C-C-T-T--------T--GA-T-A-C-T-G-GCA--C-A-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-A-T-G-----C-GA--TT-G-A------------G-GT-A-GG-C----GG--AATG-TGA-C-AT--GT-A-GCG-GTGAAA-TG-CTT-AGAC-A-TG-T-GAC--AG-A-AC-A-CC-GA--T--T--GC-GAA-G--G-C---A----G--C-T-TACCA------A-GT-CG--------------------------------------------------------------TT-A-T-T--GA--CG-----CT-GA-GG--C-A-CGA--AA-G-C--------------G-TGGG-GAG-C-A-AACA--GG-ATTA-G-ATA-C-----CC-T-G-GTA-G-T----C-CA--C-G-CCG-T-AAA--CG-ATG-AT--AA-CT---------C-GA--C--G-T-TA-G-CG-------------------------------------------------------------------------------------------ATA-CA-C----------------------------------------------------------------------------------------------------------------------------------------------------T-GT--T-A-G-C-GT-C------CA--A----GC-GAA--AG-C-G-T--T--AA-GT--T----A-TCC-ACC-T-G-GG-AAG-TA---CGA-----T-C--G-C-A-A-GGT-T--GAA-ACTC-AAA---------GGAA-TTG-ACGGG-G-G-CCCG----C-A--C-A-A-GCG-GT-G--G--AG-CA-T--GT-GGT-TT-AATT-C-G-ATG-ATAC-G-CG-A-GG-A-A-CC-TT-A-CC-AGGGC-TT-A-AA-T-G-G------------G-GAA-C-G-------------A-CC-T-T-C--TG--G-GA-A-A-C--C-A-G--A-A-T-T--T-------C----------------------------------------TTT-T------------------------------------------AG---------A---CG-GTC---C--TC---------------------------------------------------A-A-G-G-T-GCTG-CA-TGG-TT--GTC-GTC-A-GC-TC---G-TG-CC-G--TGA-GG-TGT-T-GG-G-TT-AA-GT-CCCGC-AA--------C-GAG-CGC-A-ACC-C-C-TA--CT--G-TTAG--T-T-G-C-C---AG-C-G--G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ATAATG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C----C-G------------G----G---G-A--CT---------------C-T-A-G-C-GG-A--AC-T-G-CCT--G-T------------------------------------G-----CAA----------------------------------A-C-A-GA-G-A-GG-A--AGG-T--GGGG-A-TGAC-GTC--AAAT-C---ATC-A-C-G-G-C-C-CTT----AC-G--TC-C-T-GG-GC-TA-CAC-ACGTG-C--TA--CAATG---G-CCGG-T-A--C-AGA-GG-GC--------------------------------------------------------------------------------------------------A-G-C-C-A--C-TTCG-T--G---------------------------------------A-GA-A-G-----------G--A-G-CG---A----------A--TCC-T------T---AAAGC-CG-G-T-C-T-CAG-TTC--------GGA-T-TGTAG-TC--T-GCAA-CT-C-------------------------------------------------------------------------------------------------G-ACTAC-A-T-G-AA-G-CT-GGAAT-CG-C-TA--G-TA-AT-C-G-C----GTA-TC-A-G-CC------AT--GAC-GC-G-GT-G-AAT-ACGT-T-CCCGGGCCT-TGTA----CACACCG-CCC-GTC-----A---AG--CCA-TG-GG-A--A---TT--G-GGAGT-ACC--T-AAA------G--T-CGG----------T-AA-C-C-------------------------------------------------------------G-CA-A------------------------------------------------------------------------------------------------------GG-A--G---------CC--GC--CT-AA-G-G----T-AAT-AC-CAG------------------------TG--ACT-GGGG-CT-AAG-TCGTAACAA-GGTAG-CCGT-A------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>42684
------------------------------------------------------------------------------------------------------------AGAGTTT-GA--T-CC-T-G-GCTC-AG-AT-TGAA-C-GC--TGG-C--G-GC-A-TG--C----T-T--TACACA-T-GC-A-AGT-CGG-A-CG----------G-CAG-CA-C----------------------------AGAG-G-AG----------------------------------------------------CTT-G----------------------------------------------------------------------------------CTT-CTTG----------------G-GT--G--GC--G--AG-T-GG-C-GA-A--C-------------GGG-TGAGT-G--AC-GC-A-T-C-GG---A-A--C-GT-A--C-C-GAG--TA-A------------------------------------------------------------------T-GG----GGG-AT-AA-CTG-------------------------T-C-C-----------------------GAA-A---GGA-CAG-CTAA-TA---CC-G--C-AT-A----------C--------------------G-------------------------------------CC-C-----------------------------------------------------------------------------------------------------------------------T-GA-G--------------------------------------------------------------------------------------------------------------------------------------G-G-G---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAAA--G-C-G-GG-----G--GA-T--C--------------------------------------------------------------------------------------------------------------------TTA-G----------------------------------------------------------------------------------------------------------------------G-A--CC-TC--G---C-G--------------T----T-A---T-TC-G---AG---C-G-----G-CCG-ATG--T-CTG--A------TT--A--G-CT-G----G---TTGG-C-G-GG-G-T----AAA-GG-C-C-C-ACCA--A-GG-C-G--A-CG-A------------TCA-G-T------AG-CG-G-G-TCT-G-AG----A--GG-AT--G-AT-C-CG-CCAC-A-CTGGG--A-C-TG-A-GA-C-AC-G-G-CCCAGA-CTCC-TAC-G--G-G-A-G-GC-A-GC-A-G-TG---GG-G-A-ATT-TTGGA-C-AA-T-GG--GC-GC-A----A-G-CC-T-GA-TC-CA-GCCA-TGCC-G-CG-T---G-T-C--T--GA-A-G--A--A-G-G-CC-----TT-CG---------G-G-T-T-G-T--A---AA-G-GAC--------TT-TT-G-T--C-AGG----GA-A--G---AA-AAGG---AACG-TG----T--T--AA-T---A----C-----CA-T-GTT-CTG-AT-GA-CG-GT-A-C-CT-G-AA-G---------AA-----------TAAGC-ACC-GG-C-TAA---C--T-ACGT--GCCA--G-C---A--GCCG---C-GG--TA-AT--AC---GT-AG-GGT-GCG-A-G-CG-TTAA-T-CGG-AA-TT-A--C-T--GGGC-GTA----AA-GCGG-GC--G-CA-G-A-C-G------------G--T-TA-C-T-T-AA----G-C-G-G---G-ATG-TG-A-AA-TC--CC-CGG-G--------------------------------------------------------------------CT-C-AA-------------------------------------------------------------------------CC-C-G-GG-AA-C----T-G-C-G-T-T--------C--CG-A-A-C-T-G-GGT--G-G-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-A-G-A-G-T-G-----T-GT--CA-G-A------------G-GG-G-GG-T----AG--AATT-CCA-C-GT--GT-A-GCA-GTGAAA-TG-CGT-AGAG-A-TG-T-GGA--GG-A-AT-A-CC-GA--T--G--GC-GAA-G--G-C---A----G--C-C-CCCTG------G-GA-TA--------------------------------------------------------------AC-A-C-T--GA--CG-----TT-CA-TG--C-C-CGA--AA-G-C--------------G-TGGG-TAG-C-A-AACA--GG-GTTA-G-ATA-C-----CC-T-G-GTA-G-T----C-CA--C-G-CCC-T-AAA--C-GATG-TC--GA-TT---------A-GC--T--G-T-TG-G-GG-C--A--------------------------------------------------------------------------------------CTT-GA--------------------------------------------------------------------------------------------------------------------------------------------------T-G-C-CT--T-A-G-T-AG-C------GT--A----GC-TAA--CG-C-G-T--G--AA-AT--C----G-ACC-GCC-T-G-GG-GAG-TA---CGG-----T-C--G-C-A-A-GAT-T--AAA-ACTC-AAA---------GGAA-TTG-ACGGG-G-A-CCCG----C-A--C-A-A-GCG-GT-G--G--AT-GA-T--GT-GGA-TT-AATT-C-G-ATG-CAAC-G-CG-A-AG-A-A-CC-TT-A-CC-TGGTC-TT-G-AC-A-T-G--------------TAC-G-G-------------A-AT-C-T-T--CC--A-GA-G-A-C--G-G-A--A-G-G-G--T-G-----CC-------------------------------------T--TC-G------------------------------------------GG----A----G---CC-GTA---A--CA---------------------------------------------------C-A-G-G-T-GCTG-CA-TGG-CT--GTC-GTC-A-GC-TC---G-TG-TC-G--TGA-GA-TGT-T-GG-G-TT-AA-GT-CCCGC-AA--------C-GAG-CGC-A-ACC-C-T-TG--TC--A-TTAG--T-T-G-C-C---AT-C-A--C----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTGG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T----T-G------------G----G---C-A--CT---------------C-T-A-A-T-GA-G--AC-T-G-CCG--G-T------------------------------------G-A---CAA----------------------------------A-C-C-G--G-A-GG-A--AGG-T--GGGG-A-TGAC-GTC--AAGT-C---CTC-A-T-G-G-C-C-CTT----AT-G--AC-C-A-GG-GC-TT-CAC-ACGTC-A--TA--CAATG---G-TCGG-T-A--C-AGA-GG-GT--------------------------------------------------------------------------------------------------A-G-C-C-A--A-GCCG-C--G---------------------------------------A-GG-C-G-----------G--A-G-CC---A----------A--TCC-C------A-G-AAAAC-CG-A-T-C-G-TAG-TCC--------GGA-T-TGCAC-TC--T-GCAA-CT-C-------------------------------------------------------------------------------------------------G-AGTGC-A-T-G-AA-G-TC-GGAAT-CG-C-TA--G-TA-AT-C-G-C----AGG-TC-A-G-C-------AT--ACT-GC-G-GT-G-AAT-ACGT-T-CCCGGGTCT-TGTA----CACACCG-CCC-GTC-----A---CA--CCA-TG-GG-A--G---TGG-G-GG-AT-ACC--A-GAA------G--C-AGG-TA-G-G-C-T-AA-C-C-------------------------------------------------------------G-CA-A------------------------------------------------------------------------------------------------------GG-A--GG-C--C---GC-TTG--CC--ACG-G----T-ATG-CT-TCA------------------------TG--ACT-GGGG-TG-AAG-TCGTAACAA-GGTA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>342684
------------------------------------------------------------------------------------------------------------AGAGTTT-GA--T-CC-T-G-GCTC-AG-GA-TGAA-C-GC--TAG-C--G-GC-A-GG--C----T-T--AACACA-T-GC-A-AGT-CGA-G-GG----------G-CAT-CG-C------------------------------GG-G-TA----------------------------------------------------GCA-A---------------------------------------------------------------------------------TA-C-CT------------------G-GC--G--GC--G--AC-C-GG-C-GG-A--A-------------GGG-TGCGT-A--AC-GC-G-T-G-AG---CGA--C-AT-A--C-C-CGT--GA-C------------------------------------------------------------------A-GG----GGG-AT-AA-CAG-------------------------A-T-G-----------------------GAA-A---CGT-CTC-CTAA-TA---CC-C--C-AT-A----------A--------------------G-A--T-C-A--T--A-----------------TA---TC-G-------------------------------------------------------------------------------------------------------------------------CA-T--------------------------------------------------------------------------------------------------------------------------------------G-G-T--A-T---------------G--T-G-A-T-T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAAA--G-G----------------------------------------------------------------------------------------------------------------------------------------TGA-G----------------------------------------------------------------------------------------------------------------------------------A-ACC-G--------------G----T-C---A-CG-G---AT---T-G-----G-CTC-GCG--T-CCC--A------TC--A--G-GT-A----G---ACGG-C-G-GG-G-C----AGC-GG-C-C-C-GCCG--T-GC-C-G--A-CG-A------------CGG-G-T------AG-GG-G-C-TCT-G-A-G---A--GG-AG--T-GA-C-CC-CCAC-A-ATGGA--A-C-TG-A-GA-C-AC-G-G-TCCATA-CTCC-TAC-G--G-G-A-G-GC-A-GC-A-G-TG---AG-G-A-ATA-TTGGT-C-AA-T-GG--GC-GG-A----A-G-CC-T-GA-AC-CA-GCCA-TGCC-G-CG-T---G-C-G--G--GA-G-G--A--C-G-G-CCC----TA-TG---------G-G-T-T-G-T--A---AA-C-CGC--------TT-TT-G-A--G-TGA----GA-G--C---AATAAGG---TTCA----------C--GT-G-----------------TG-GA-CCG-AT-GA-GA-GT-A-T-CA-T-TC-G---------AA-----------TAAGC-ATC-GG-C-TAA---C--T-CCGT--GCCA--G-C---A--GCCG---C-GG--TA-AT--AC---GG-AG-GAT-GCG-A-G-CG-TTAT-C-CGG-AT-TC-A--T-T--GGGT-TTA----AA-GGGT-GC--G-TA-G-G-C-G------------G--A-CA-T-G-T-AA----G-T-C-C---G-AGG-TG-A-AA-GA--CC-GGG-G--------------------------------------------------------------------CC-C-AA-------------------------------------------------------------------------CC-C-C-GG-GG-T----T-G-C-C-T-C--------G--GA-T-A-C-T-G-TGT--G-T-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-G-G-A-G-T-G-----G-AC--GT-G-C------------C-GC-C-GG-G----GG--AATG-AGT-G-GT--GT-A-GCG-GTGAAA-TG-CAT-AGAT-G-TC-A-CTC--AG-A-AC-A-CC-GA--T--T--GC-GAA-G--G-C---A----C--C-T-GGCGA------A-TG-TC--------------------------------------------------------------TT-A-C-T--GA--CG-----CT-GA-GG--C-A-CGA--AA-G-C--------------G-TGGG-GAT-C-G-AACA--GG-ATTA-G-ATA-C-----CC-T-G-GTA-G-T----C-CA--C-G-CAG-T-AAA--CG-ATG-AT--GG-CT---------G-TC--C--G-T-TC-G-CT------------------------------------------------------------------------------------------CCGATAG-G----------------------------------------------------------------------------------------------------------------------------------------------------A-GT--G-A-G-T-AG-A------CA--A----GC-GAA--AG-C-G-C--T--AA-GC--C----A-TCC-ACC-T-G-GG-GAG-TA---CGG-----C-C--G-C-A-A-GGC-T--GAA-ACTC-AAA---------GGAA-TTG-ACGGG-G-G-CCCG----C-A--C-A-A-GCG-GA-G--G--AA-CA-T--GT-GGT-TT-AATT-C-G-ATG-ATAC-G-CG-A-GG-A-A-CC-TT-A-CC-CGGGC-TC-G-AA-C-G-G------------C-AGG-T-G-------------A-AC-G-A-T--GC--A-GA-G-A-T--G-C-A--A-A-G-G----------CC-----------------------------------CTT--CG--------------------------------------------GG---------G---CG-TCT---G--TC---------------------------------------------------G-A-G-G-T-GCTG-CA-TGG-TT--GTC-GTC-A-GC-TC---G-TG-CC-G--TGA-GG-TGT-C-GG-C-TC-AA-GT-GCCAT-AA--------C-GAG-CGC-A-ACC-C-T-TG--CC--T-GCAG--T-T-G-C-C---AT-C-G--G----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTAAAG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------C----C-G------------G----G---G-A--CT---------------C-T-G-C-A-GG-G--AC-T-G-CCA--C-C------------------------------------G-----CAA----------------------------------G-G-T-GA-G-A-GG-A--GGG-G--GGGG-A-TGAC-GTC--AAAT-C---AGC-A-C-G-G-C-C-CTT----AC-G--TC-C-G-GG-GC-GA-CAC-ACGTG-T--TA--CAATG---G-CGGC-C-A--C-AGC-GG-GA--------------------------------------------------------------------------------------------------A-G-C-C-A--C-CCAG-T--G---------------------------------------A-TG-G-G-----------G--C-G-CG---G----------A--TCC-C-----AA---AAAAG-CC-G-C-C-T-CAG-TTC--------GGA-T-CGGAG-TC--T-GCAA-CC-C-------------------------------------------------------------------------------------------------G-ACTCC-G-T-G-AA-G-CT-GGATT-CG-C-TA--G-TA-AT-C-G-C----GCA-TC-A-G-CC------AT--GGC-GC-G-GT-G-AAT-ACGT-T-CCCGGGCCT-TGTA----CACACCG-CCC-GTC-----A---AG--CCA-TG-GG-A--G---TCG-T-GG-GC-GCC--T-GAA------G--G-CCG----------T-GA-C-C-------------------------------------------------------------G-CG-A------------------------------------------------------------------------------------------------------GG-A--G----------C-GGC--CT--AGG-G----C-GAA-CG-CGG------------------------TG--ACT-GGGG-CT-AAG-TCGTAACAA-GGTAA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>295053
------------------------------------------------------------------------------------------------------------AGAGTTT-GA--T-CC-T-G-GCT-CAG-GA-CGAA-C-GC--TGG-C--G-GC-G-TG--C----C-T--AACACA-T-GC-A-AGT-CGA-A-CG----------G---------------------------------------AG-A-TG----------------------------------------------------CTC-C----------------------------------------------------------------------------------TTC-GGGA----------------G-TA--T--CT--T--AG-T-GG-C-GA-A--C-------------GGG-TGAGT-A--AC-GC-G-T-G-AG---C-A--ACCT-G--A-C-CTT--CA-C------------------------------------------------------------------A-GG----GGG-AT-AA-CCG-------------------------C-T-G-----------------------GAA-A---CAG-CAG-CTAA-TA---CC-G--C-AT-A----------A--------------------C-------------------------------------GT-C-----------------------------------------------------------------------------------------------------------------------G-CA-A--------------------------------------------------------------------------------------------------------------------------------------G-A-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAAA--G-A-G-GG-----G--GA-C--C--------------------------------------------------------------------------------------------------------------------TTC-G----------------------------------------------------------------------------------------------------------------------G-G--CC-TC--T---T-G--------------C----C-A---T-CG-G---AT---G-T-----G-CCC-AGA--T-GGG--A------TT--A--G-CT-T----G---TTGG-T-G-GG-G-T----AAC-GG-C-T-C-ACCA--A-GG-C-G--A-CG-A------------TCC-C-T------AG-CT-G-G-TCT-G-AG----A--GG-AT--G-AC-C-AG-CCAC-A-CTGGA--A-C-TG-A-GA-C-AC-G-G-TCCAGA-CTCC-TAC-G--G-G-A-G-GC-A-GC-A-G-TG---GG-G-A-ATA-TTGCA-C-AA-T-GG--GC-GC-A----A-G-CC-T-GA-TG-CA-GCCA-TGCC-G-CG-T---G-T-A--T--GA-A-G--A--A-G-G-CC-----TT-CG---------G-G-T-T-G-T--A---AA-G-TAC--------TT-TC-A-G--C-GGG----GA-G--G---AA-GGGA---GTAA-AG----T--T--AA-T---A----C-----CT-T-TGC-TCA-TT-GA-CG-TT-A-C-CC-G-CA-G---------AA-----------GAAGC-ACC-GG-C-TAA---C--T-CCGT--GCCA--G-C---A--GCCG---C-GG--TA-AT--AC---GG-AG-GGT-GCA-A-G-CG-TTAA-T-CGG-AA-TT-A--C-T--GGGC-GTA----AA-GCGC-AC--G-CA-G-G-C-G------------G--T-TT-G-T-T-AA----G-T-C-A---G-ATG-TG-A-AA-TC--CC-CGG-G--------------------------------------------------------------------CT-C-AA-------------------------------------------------------------------------CC-T-G-GG-AA-C----T-G-C-A-T-C--------T--GA-T-A-C-T-G-GCA--A-G-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T-C-----T-CG--TA-G-A------------G-GG-G-GG-T----AG--AATT-CCA-G-GT--GT-A-GCG-GTGAAA-TG-CGT-AGAG-A-TC-T-GGA--GG-A-AT-A-CC-GG--T--G--GC-GAA-G--G-C---G----G--C-C-CCCTG------G-AC-GA--------------------------------------------------------------AG-A-C-T--GA--CG-----CT-CA-GG--T-G-CGA--AA-G-C--------------G-TGGG-GAG-C-A-AACA--GG-ATTA-G-ATA-C-----CC-T-G-GTA-G-T----C-CA--C-G-CCG-T-AAA--C-GATG-TC--GA-CT---------T-GG--A--G-G-TT-G-TG-C--C--------------------------------------------------------------------------------------CTT-GA--------------------------------------------------------------------------------------------------------------------------------------------------G-G-C-GT--G-G-C-T-TC-C------GG--A----GC-TAA--CG-C-G-T--T--AA-GT--C----G-ACC-GCC-T-G-GG-GAG-TA---CGG-----C-C--G-C-A-A-GGT-T--AAA-ACTC-AAA---------TGAA-TTG-ACGGG-G-G-CCCG----C-A--C-A-A-GCG-GT-G--G--AG-CA-T--GT-GGT-TT-AATT-C-G-ATG-CAAC-G-CG-A-AG-A-A-CC-TT-A-CC-TGGTC-TT-G-AC-A-T-C--------------CAC-A-G-------------A-AC-T-T-T--CC--A-GA-G-A-T--G-G-A--T-T-G-G--T-G-----CC-------------------------------------T--TC-G------------------------------------------GG----A----A---CT-GTG---A--GA---------------------------------------------------C-A-G-G-T-GCTG-CA-TGG-CT--GTC-GTC-A-GC-TC---G-TG-TT-G--TGA-AA-TGT-T-GG-G-TT-AA-GT-CCCGC-AA--------C-GAG-CGC-A-ACC-C-T-TG--TC--C-TTTG--T-T-G-C-C---AG-C-G--G--T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CCG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------G----C----C-G------------G----G---A-A--CT---------------C-A-A-A-G-GA-G--AC-T-G-CCA--G-T------------------------------------G-A---TAA----------------------------------A-C-T-G--G-A-GG-A--AGG-T--GGGG-A-TGAC-GTC--AAGT-C---ATC-A-T-G-G-C-C-CTT----AC-G--AC-C-A-GG-GC-TA-CAC-ACGTG-C--TA--CAATG---G-CGCA-T-A--C-AAA-GA-GA--------------------------------------------------------------------------------------------------A-G-C-G-A--C-CTCG-C--G---------------------------------------A-GA-G-C-----------A--A-G-CG---G----------A--CCT-C------A-T-AAAGT-GC-G-T-C-G-TAG-TCC--------GGA-T-TGGAG-TC--T-GCAA-CT-C-------------------------------------------------------------------------------------------------G-ACTCC-A-T-G-AA-G-TC-GGAAT-CG-C-TA--G-TA-AT-C-G-T----GGA-TC-A-G--A------AT--GCC-AC-G-GT-G-AAT-ACGT-T-CCCGGGCCT-TGCA----CACACCG-CC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
>879972
-----------------------------------------------------------------------------------------------------------------------------------------GA-CGAA-C-GC--TGG-C--G-GC-G-TG--C----C-T--AATACA-T-GC-A-AGT-CGA-A-CGA---------G--A---T--------------TG--------------ACCG-G-TG----------------------------------------------------CTT-G----------------------------------------------------------------------------------CAC-TGGTCA----------------A------TC--T--AG-T-GG-C-GA-A--C-------------GGG-TGAGT-A--AC-AC-G-T-G-GG---TAA--C-CT-G--C-C-CAT--CA-G------------------------------------------------------------------A-GG----GGG-AT-AA-CAT-------------------------T-C-G-----------------------GAA-A---CGG-ATG-CTAA-AA---CC-G--C-AT-A----------G--------------------G-T--C-T-T--C--G-----------------A----AC-C-----------------------------------------------------------------------------------------------------------------------G-CA-T--------------------------------------------------------------------------------------------------------------------------------------G-G-T----T---------------T--G-A-A-G-A------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGAAA--A-G-AGGC-----------------------------------------------------------------------------------------------------------------------------------GCA-A----------------------------------------------------------------------------------------------------------------------------G-CT--T---C-T-------------GC----T-G---A-TG-G---AT---G-G-----A-CCC-GCG--G-TGT--A------TT--A--G-CT-A----G---TTGG-T-G-GG-G-T----AAC-GG-C-T-C-ACCA--A-GG-C-G--A-CG-A------------TAC-A-T------AG-CC-G-A-CCT-G-AG----A--GG-GT--G-AT-C-GG-CCAC-A-CTGGG--A-C-TG-A-GA-C-AC-G-G-CCCAGA-CTCC-TAC-G--G-G-A-G-GC-A-GC-A-G-TA---GG-G-A-ATC-TTCGG-C-AA-T-GG--AC-GG-A----A-G-TC-T-GA-CC-GA-GCAA-CGCC-G-CG-T---G-A-G--T--GA-A-G--A--A-G-G-TT-----TT-CG---------G-A-T-C-G-T--A---AA-G-CTC--------TG-TT-G-T--A-AGA----GA-A--G---AACGAGT---GTGA-GA----G--T--GG-A---A----A-----GT-T-CAC-ACT-GT-GA-CG-GT-A-T-CT-T-AC-C---------AG-----------AAAGG-GAC-GG-C-TAA---C--T-ACGT--GCCA--G-C---A--GCCG---C-GG--TA-AT--AC---GT-AG-GTC-CCG-A-G-CG-TTGT-C-CGG-AT-TT-A--T-T--GGGC-GTA----AA-GCGA-GC--G-CA-G-G-C-G------------G--T-TA-G-A-T-AA----G-T-C-T---G-AAG-TT-A-AA-GG--CT-GTG-G--------------------------------------------------------------------CT-T-AA-------------------------------------------------------------------------CC-A-T-AG-TA-C------G-C-T-T-T--------G--GA-A-A-C-T-G-TTT--A-A-C---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-T-G-A-G-T-G-----C-AA--GA-G-G------------G-GA-G-AG-T----GG--AATT-CCA-T-GT--GT-A-GCG-GTGAAA-TG-CGT-AGAT-A-TA-T-GGA--GG-A-AC-A-CC-GG--T--G--GC-GAA-A--G-C---G----G--C-T-CTCTG------G-CT-TG--------------------------------------------------------------TA-A-C-T--GA--CG-----CT-GA-GG--C-T-CGA--AA-G-C--------------G-TGGG-GAG-C-A-AACA--GG-ATTA-G-ATA-C-----CC-T-G-GTA-G-T----C-CA--C-G-CCG-T-AAA--C-GATG-AG--TG-CT---------A-GG--T--G-T-TA-G-AC-C--C--T-----------------------------------------------------------------------------------TTC-CG--------------------------------------------------------------------------------------------------------------------------------------------------G-G-G-TT--T-A-G-T-GC-C------GC--A----GC-TAA--CG-C-A-T--T--AA-GC--A----C-TCC-GCC-T-G-GG-GAG-TA---CGA-----C-C--G-C-A-G-GGT-T--GAA-ACTC-AAA---------GGAA-TTG-ACGGG-G-G-CCCG----C-A--C-A-A-GCG-GT-G--G--AG-CA-T--GT-GGT-TT-AATT-C-G-AAG-CAAC-G-CG-A-AG-A-A-CC-TT-A-CC-AGGTC-TT-G-AC-A-TCC--------------CTC-T--------------GA-CC-G-C-T--CT--A-GA-G-A-T--A-G-A--G-C-T-T--T-------CC-------------------------------------T--TC-G------------------------------------------GG---------A---CA-GAGG--T--GA---------------------------------------------------C-A-G-G-T-GGTG-CA-TGG-TT--GTC-GTC-A-GC-TC---G-TG-TC-G--TGA-GA-TGT-T-GG-G-TT-AA-GT-CCCGC-AA--------C-GAG-CGC-A-ACC-C-C-TA--TT--G-TTAG--T-T-G-C-C---AT-C-A-----T-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T--------T-G------------G----G---C-A--CT---------------C-T-A-G-C-GA-G--AC-T-G-CCG--G-T------------------------------------A-A---TAA----------------------------------A-C-C-G--G-A-GG-A--AGG-T--GGGG-A-TGAC-GTC--AAAT-C---ATC-A-T-G-C-C-C-CTT----AT-G--AC-C-T-GG-GC-TA-CAC-ACGTG-C--TA--CAATG---G-CTGG-T-A--C-AAC-GA-GT--------------------------------------------------------------------------------------------------C-G-C-A-A--G-CCGG-T--G---------------------------------------A-CG-G-C-----------A--A-G-CT---A----------A--TCT-C------T-T-AAAGC-CA-G-T-C-T-CAG-TTC--------GGA-T-TGTAG-GC--T-GCAA-CT-C-------------------------------------------------------------------------------------------------G-CCTAC-A-T-G-AA-G-TC-GGAAT-CG-C-TA--G-TA-AT-C-G-C----GGA-TC-A-G-C-------AC--GCC-GC-G-GT-G-AAT-ACGT-T-CCCGGGCCT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Raw
refseqs_aligned_lanemask.txt
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Raw
refseqs_tax.txt
426848 k__Bacteria; p__Bacteroidetes; c__VC2_1_Bac22; o__; f__; g__; s__
42684 k__Bacteria; p__Proteobacteria; c__Betaproteobacteria; o__Neisseriales; f__Neisseriaceae; g__; s__
342684 k__Bacteria; p__Bacteroidetes; c__Bacteroidia; o__Bacteroidales; f__; g__; s__
295053 k__Bacteria; p__Proteobacteria; c__Gammaproteobacteria; o__Enterobacteriales; f__Enterobacteriaceae; g__; s__
879972 k__Bacteria; p__Firmicutes; c__Bacilli; o__Lactobacillales; f__Streptococcaceae; g__Streptococcus; s__
Raw
seqs.fna
>f2_1271 HWI-EAS440_0386:1:30:4487:20156#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCG
>f2_1539 HWI-EAS440_0386:1:31:12039:10494#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_2278 HWI-EAS440_0386:1:32:3943:19113#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_2349 HWI-EAS440_0386:1:33:11754:2337#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_2727 HWI-EAS440_0386:1:33:17206:16370#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_2750 HWI-EAS440_0386:1:33:4173:17707#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_2919 HWI-EAS440_0386:1:34:7244:5240#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_2990 HWI-EAS440_0386:1:34:15559:8361#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_3856 HWI-EAS440_0386:1:36:7847:3654#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_4379 HWI-EAS440_0386:1:37:18509:5405#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_4866 HWI-EAS440_0386:1:38:15872:7019#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f2_4913 HWI-EAS440_0386:1:38:5446:9251#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGGCATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATACTTGAGTGCCGGAGGGGTAAGCGGAATTTCTAGTG
>f2_5255 HWI-EAS440_0386:1:39:7428:2824#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_5313 HWI-EAS440_0386:1:39:18251:4560#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_6581 HWI-EAS440_0386:1:41:12697:18427#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_6654 HWI-EAS440_0386:1:42:18390:1542#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCG
>f1_6871 HWI-EAS440_0386:1:42:12648:9905#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_7004 HWI-EAS440_0386:1:42:12524:15765#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_8307 HWI-EAS440_0386:1:45:2418:10930#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f1_8746 HWI-EAS440_0386:1:46:3313:8982#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f2_8835 HWI-EAS440_0386:1:46:15230:11989#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>f1_10106 HWI-EAS440_0386:1:49:11503:8253#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_10228 HWI-EAS440_0386:1:49:10428:12536#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAATCTTGAGTTCGGACAAGGCAGATGG
>f2_10741 HWI-EAS440_0386:1:50:19354:13842#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_11370 HWI-EAS440_0386:1:52:13612:2374#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_11949 HWI-EAS440_0386:1:53:11869:5956#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_12151 HWI-EAS440_0386:1:53:15228:14924#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>f2_12169 HWI-EAS440_0386:1:53:6500:15595#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_12885 HWI-EAS440_0386:1:55:6617:6505#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_13027 HWI-EAS440_0386:1:55:2587:12315#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_13201 HWI-EAS440_0386:1:55:8634:19786#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_13503 HWI-EAS440_0386:1:56:16989:11768#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_13697 HWI-EAS440_0386:1:56:12011:20385#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_14665 HWI-EAS440_0386:1:59:14413:3423#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA
>f1_17256 HWI-EAS440_0386:1:66:9612:13415#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_17407 HWI-EAS440_0386:1:67:5154:3682#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_18550 HWI-EAS440_0386:1:70:6653:12933#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_19304 HWI-EAS440_0386:1:72:14543:4190#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f2_20011 HWI-EAS440_0386:1:73:5930:19960#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCG
>f2_20101 HWI-EAS440_0386:1:74:13281:8786#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f2_21396 HWI-EAS440_0386:1:79:15005:5440#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>f2_23683 HWI-EAS440_0386:1:84:18937:10979#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>f2_24507 HWI-EAS440_0386:1:86:4702:6973#0/1 orig_bc=ACCAGACGATGC new_bc=ACCAGACGATGC bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f1_24744 HWI-EAS440_0386:1:86:13607:15613#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>p1_27798 HWI-EAS440_0386:2:37:6298:2924#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_28761 HWI-EAS440_0386:2:40:8922:18632#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_29120 HWI-EAS440_0386:2:42:1275:11504#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_29150 HWI-EAS440_0386:2:42:9433:13371#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_29350 HWI-EAS440_0386:2:43:17387:8549#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_29474 HWI-EAS440_0386:2:43:7240:17422#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_30113 HWI-EAS440_0386:2:46:8095:9920#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAG
>p1_30198 HWI-EAS440_0386:2:46:7650:15609#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAGAAGGGG
>p2_30279 HWI-EAS440_0386:2:47:19039:4475#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_30549 HWI-EAS440_0386:2:48:14520:4777#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCAGACGGTTACTTAAGCGGGATGTGAAATCCCCGGGCTCAACCCGGGAACTGCGTTCCGAACTGGGTG
>p1_30611 HWI-EAS440_0386:2:48:11791:9189#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_31582 HWI-EAS440_0386:2:52:8533:3351#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTG
>p2_31615 HWI-EAS440_0386:2:52:8097:5047#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGG
>p1_32061 HWI-EAS440_0386:2:53:12650:19528#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_32401 HWI-EAS440_0386:2:55:1617:7585#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_32437 HWI-EAS440_0386:2:55:11012:9838#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_32494 HWI-EAS440_0386:2:55:10467:14158#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_32628 HWI-EAS440_0386:2:56:18987:4105#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGGCATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATACTTGAGTGCCGGAG
>p1_32652 HWI-EAS440_0386:2:56:3581:5687#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_33176 HWI-EAS440_0386:2:58:7254:5181#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>p2_33268 HWI-EAS440_0386:2:58:13656:11052#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATGTCTTGAGTGCAGTTG
>p2_34392 HWI-EAS440_0386:2:62:3660:12239#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>p1_34397 HWI-EAS440_0386:2:62:8256:12723#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_34773 HWI-EAS440_0386:2:64:10707:4587#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAGAAGGGGAGAG
>p2_34872 HWI-EAS440_0386:2:64:5256:11455#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAG
>p2_35125 HWI-EAS440_0386:2:65:8479:13841#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAAGGACCAGGAGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGC
>p2_35617 HWI-EAS440_0386:2:67:2652:17993#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTT
>p2_35810 HWI-EAS440_0386:2:68:17310:15141#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAG
>p1_35931 HWI-EAS440_0386:2:69:13384:7891#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_36300 HWI-EAS440_0386:2:70:17775:16457#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TTCGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGATGTGAAAGGCATGGGCTCAACCTGTGGACTGCATTGGAAACTGTCATACT
>p2_36352 HWI-EAS440_0386:2:71:4992:2947#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>p2_36441 HWI-EAS440_0386:2:71:7655:9093#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAAGTGAAATACCCGGGCTCAACCTGGGAACTGCTTTGGAAACTGTTGTTCTAGAGTGTTG
>p2_37291 HWI-EAS440_0386:2:75:2654:2930#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_37292 HWI-EAS440_0386:2:75:15500:3047#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_37427 HWI-EAS440_0386:2:75:17389:13227#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_37793 HWI-EAS440_0386:2:77:16603:2466#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_37880 HWI-EAS440_0386:2:77:11070:8817#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_37985 HWI-EAS440_0386:2:77:8401:16367#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_38017 HWI-EAS440_0386:2:77:13529:19101#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAAGTGAAATACCCGGGCTCAACCTGGGAACTGCTTTGGAAACTGTTGTTCTAGGCTGTTGGAGAGGTAAGTGGAATTCCTGGTG
>p2_38493 HWI-EAS440_0386:2:80:12905:9503#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_38528 HWI-EAS440_0386:2:80:13500:11791#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p1_39127 HWI-EAS440_0386:2:84:11417:3032#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>p2_39175 HWI-EAS440_0386:2:84:12513:6706#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGG
>p1_39254 HWI-EAS440_0386:2:84:5143:12808#0/1 orig_bc=AACGCACGCTAG new_bc=AACGCACGCTAG bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAAG
>t2_52630 HWI-EAS440_0386:5:48:16990:6975#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAG
>t2_52670 HWI-EAS440_0386:5:48:11227:8602#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>t2_52671 HWI-EAS440_0386:5:48:12051:8637#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGG
>t2_52739 HWI-EAS440_0386:5:48:14315:11448#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAA
>t2_52841 HWI-EAS440_0386:5:48:3257:15721#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGG
>t1_52964 HWI-EAS440_0386:5:52:9778:2855#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCC
>t2_52970 HWI-EAS440_0386:5:52:6994:3157#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>t1_53038 HWI-EAS440_0386:5:52:14917:5613#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGG
>t1_53176 HWI-EAS440_0386:5:52:6593:11349#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTT
>t2_53194 HWI-EAS440_0386:5:52:3945:11982#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGC
>t2_53250 HWI-EAS440_0386:5:52:9966:14855#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>t2_53290 HWI-EAS440_0386:5:52:5130:16397#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGC
>t1_53329 HWI-EAS440_0386:5:52:14413:18238#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCG
>t2_53395 HWI-EAS440_0386:5:53:4294:2974#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>t2_53531 HWI-EAS440_0386:5:53:17708:9105#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAG
>t2_53719 HWI-EAS440_0386:5:53:15322:17370#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCA
>t2_53750 HWI-EAS440_0386:5:53:12367:19097#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAG
>t2_53764 HWI-EAS440_0386:5:54:2344:1948#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAA
>t1_53967 HWI-EAS440_0386:5:54:10544:11545#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGGAA
>t1_54144 HWI-EAS440_0386:5:54:12690:20621#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTT
>t2_54418 HWI-EAS440_0386:5:55:17299:14062#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTAC
>t1_54475 HWI-EAS440_0386:5:55:18045:16412#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGGAA
>t1_54579 HWI-EAS440_0386:5:57:5661:1897#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCC
>t2_55465 HWI-EAS440_0386:5:59:3521:5889#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGCCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGGAA
>t1_55525 HWI-EAS440_0386:5:59:8117:8387#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCC
>t1_55836 HWI-EAS440_0386:5:60:17033:2188#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGGAA
>t2_56059 HWI-EAS440_0386:5:60:14779:12961#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGG
>t2_56143 HWI-EAS440_0386:5:60:10116:17219#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGG
>t1_56191 HWI-EAS440_0386:5:60:12195:19960#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTG
>t1_56398 HWI-EAS440_0386:5:62:1764:10337#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTG
>t1_56590 HWI-EAS440_0386:5:62:14521:17922#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCG
>t2_56639 HWI-EAS440_0386:5:65:15675:2221#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAA
>t1_56713 HWI-EAS440_0386:5:65:2032:5786#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTT
>t1_56790 HWI-EAS440_0386:5:65:1863:8992#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTT
>t2_56827 HWI-EAS440_0386:5:65:12520:10388#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAGAAGGGG
>t1_56940 HWI-EAS440_0386:5:65:19417:14992#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTT
>t1_57060 HWI-EAS440_0386:5:65:14396:20229#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTT
>t2_57101 HWI-EAS440_0386:5:71:11781:3199#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAA
>t1_57105 HWI-EAS440_0386:5:71:4972:3447#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAAGTTAAAGGCTGTGGCTCAACCATAGTTCGCTTTGGAAACTGTCAAACTTGAGTGCAGAAGGGGAGAG
>t1_57179 HWI-EAS440_0386:5:71:12024:6939#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTG
>t2_57248 HWI-EAS440_0386:5:71:7274:9435#0/1 orig_bc=ATACTATTGCGC new_bc=ATACTATTGCGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGG
>t1_57269 HWI-EAS440_0386:5:71:9902:10255#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTTGAGTACGCACAACGTTGGCGGAA
>t1_57351 HWI-EAS440_0386:5:71:12017:13776#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAAGTTAAAGGCTGTGGCTTAACCATAGTACGCTTTGGAAACTGTTTAACTTGAGTGCAGAAGGGG
>t1_57363 HWI-EAS440_0386:5:71:14597:14338#0/1 orig_bc=AGTGAGAGAAGC new_bc=AGTGAGAGAAGC bc_diffs=0
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCTGGAGATTAAGTGTGTTGTGAAATGTAGACGCTCAACGTCTGAATTGCAGCGCATACTGGTTTCCTT
>t1_1 random.sequence1
CTTCCGCGGAGCACCTGTATGCGAGTTATACTAATATCGTACAGCACCGATAGACAGTCTTCACTTACACCAAACTCTTACCACTGTGCCCCTGCAGTTTTTCTTGATGCTACAACTAACTCATCGACAGT
>p1_2 random.sequence2
TTGTTATAAGTTTCTGATATTTAATTTCGTAAATAGTGGTTCCAACCTACCTCCGTAAAGTATACCGAGATGAAACTTCCCACGGCTCGGGCGATGATGACGTGTGGACATCGGAGCGCCGGTCCGGATGC
>f3_130 f1_2278 HWI-EAS440_0386:1:32:3943:19113#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_131 f1_2727 HWI-EAS440_0386:1:33:17206:16370#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_132 f1_2919 HWI-EAS440_0386:1:34:7244:5240#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_133 f1_2990 HWI-EAS440_0386:1:34:15559:8361#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_134 f1_3856 HWI-EAS440_0386:1:36:7847:3654#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_135 f1_4379 HWI-EAS440_0386:1:37:18509:5405#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_136 f1_4866 HWI-EAS440_0386:1:38:15872:7019#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f3_137 f1_6581 HWI-EAS440_0386:1:41:12697:18427#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_138 f1_6871 HWI-EAS440_0386:1:42:12648:9905#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_139 f1_7004 HWI-EAS440_0386:1:42:12524:15765#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_140 f1_8307 HWI-EAS440_0386:1:45:2418:10930#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f3_141 f1_8746 HWI-EAS440_0386:1:46:3313:8982#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f3_142 f1_10106 HWI-EAS440_0386:1:49:11503:8253#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_143 f1_11949 HWI-EAS440_0386:1:53:11869:5956#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_144 f1_12885 HWI-EAS440_0386:1:55:6617:6505#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_145 f1_13027 HWI-EAS440_0386:1:55:2587:12315#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_146 f1_13697 HWI-EAS440_0386:1:56:12011:20385#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_147 f1_14665 HWI-EAS440_0386:1:59:14413:3423#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAA
>f3_148 f1_17256 HWI-EAS440_0386:1:66:9612:13415#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_149 f1_18550 HWI-EAS440_0386:1:70:6653:12933#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f3_150 f1_19304 HWI-EAS440_0386:1:72:14543:4190#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGCGGTAAAATCGTGCGGCTCAACCGTACCGTGCCGTTGAAACTGGCAACCTTGAGTTCGGACAAGGCAGATGGAATTCGTGGTGT
>f3_151 f1_24744 HWI-EAS440_0386:1:86:13607:15613#0/1 orig_bc=ACACTGTTCATG new_bc=ACACTGTTCATG bc_diffs=0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAG
>not16S.1_130
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_131
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_132
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_133
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_134
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_135
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_136
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_137
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_138
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_139
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_140
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_141
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_142
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_143
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_144
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_145
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_146
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_147
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_148
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_149
GAGAAGATGCGGAGTCAATCATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_150
GTTGGTTTATGACGACGTCCGACATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>not16S.1_151
GTTGGTTTATGACGACGTCCGACATTACGTGCCAAGGTAGTCATTACCCATCGCGGGTGCCCGTGCATGCAAGGTGTTTGGCTGGAGGCCTACTCGGGGAATATCATAACCGCAGACAGTCAATACCTATCAGA
>f4_0
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_1
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_2
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_3
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_4
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_5
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_6
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_7
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_8
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_9
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_10
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_11
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_12
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_13
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_14
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_15
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_16
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_17
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_18
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_19
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_20
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
>f4_21
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAG
Raw
ucr_params.txt
pick_otus:otu_picking_method uclust_ref
pick_otus:refseqs_fp $PWD/ref.fna
Raw
ucrss_params.txt
pick_otus:max_rejects 8
pick_otus:word_length 8
pick_otus:max_accepts 1
pick_otus:stepwords 8
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