Twitter abuses all media file uploads, each type in its own way. If we want to upload a good looking animation loop from some low-color, high-detail generative art, we have to game their system's mechanisms.
don't upload a video file, they will re-encode it into absolute
create a GIF, which they will auto-convert into a video file
The frames of the GIF will be resized to an even-sized width using an extremely naive algorithm. Your GIF should be an even size (1000, 2000, &c) so the multiple of this size to avoid janky artifacts caused by floating point division.
The GIF format is also terrible, so you have to game that too. In particular, if we create a GIF using ffmpeg's defaults we end up with one small divergent color palette per frame. This leads to weird inconsistencies and artifacts between frames and blows up the file size. To avoid this, choose a representative frame from your animation and generate a palette from that:
ffmpeg -i frame-030.png -vf palettegen -y palette.png
And then generate your GIF using that palette:
ffmpeg -framerate 20 -pattern_type glob -i 'frame-*.png' -i palette.png -filter_complex "paletteuse" art.gif
If the GIF looks right, but is slightly too large to upload, you can give
gifsicle a try:
gifsicle -O art.gif > art-optimized.gif
... et Voilà! Your beautiful art is as Twitter ready as it can be. Vaya con dios!