This program reverses your video using opencv, and saves it with mp4 content.

reverse_video_save.py

# make sure you have opencv installed, "pip install opencv-python"
# make sure your video is .avi or .mp4 encoded, else change the encoder accordingly
import cv2 
output_file = "output.mp4"
cap = cv2.VideoCapture("Video File Location") 
width = int(cap.get(cv2.CAP_PROP_FRAME_WIDTH))
height = int(cap.get(cv2.CAP_PROP_FRAME_HEIGHT))
fps =  cap.get(cv2.CAP_PROP_FPS)
check , vid = cap.read() 
counter = 0
check = True
video_type_cv2 = cv2.VideoWriter_fourcc(*'XVID')
out = cv2.VideoWriter(output_file,video_type_cv2,int(fps),(width,height))
frame_list = [] 
while(check == True):  
    check , vid = cap.read() 
    frame_list.append(vid) 
    counter += 1
    

frame_list.pop() 
frame_list.reverse()
for frame in frame_list:
  out.write(frame)

cap.release()
out.release()
cv2.destroyAllWindows()