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Combine heterozygous DNA sequences into one sequence that reflects ambiguities
# For any two DNA sequences, this code will generate a single sequence which represents differences among them with the IUPAC ambiguities.
het.cbm <- function(x1, x2){
if (length(x1)!=length(x2)) stop("sequences are not the same length!")
oot <- paste(x1, x2, sep="")
# define nucleotides
nucleotides<-c("A", "C", "G", "T")
# make a matrix of all possible combinations of nucleotides
combos <- matrix(NA, 4,4)
for(i in 1:length(nucleotides)){
for(j in 1:length(nucleotides)){
combos[i,j] <- paste(nucleotides[i], nucleotides[j], sep="")
# The IUPAC ambiguity codes (ordered appropriately according to our matrix- by columns then rows)
IUPAC <- c("A", "M", "R", "W", "M", "C", "S", "Y", "R", "S", "G", "K", "W", "Y", "K", "T")
# put that into a matrix format (might not be necessary)
outie <- matrix(IUPAC, 4,4)
dimnames(outie) <- list(nucleotides,nucleotides)
# What is the IUPAC code for each of the elements of our pasted together sequence?
# outie[match(oot, combos)]
# IUPAC[match(oot, combos)]
yep <- outie[match(oot, combos)]
# Example:
fakedata<-c("A","C","G","T","A","T") # Make up a DNA sequence
seq1<-c(fakedata,fakedata) # paste it end to end
seq2<-c(fakedata,rev(fakedata)) # paste the forward and reverse sequence end to end (so we're sure we'll have differences)
# R A or G
# Y C or T
# S G or C
# W A or T
# K G or T
# M A or C
# N any base
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