gistfile1.txt

maasha@maxwell:~/data/qiime_tutorial-v1.5.0$ pick_otus_through_otu_table.py -i TEST/split_library_output/seqs.fna -o TEST/otus
Traceback (most recent call last):
  File "/home/maasha/install/QIIME1.6/bin/pick_otus_through_otu_table.py", line 131, in <module>
    main()    
  File "/home/maasha/install/QIIME1.6/bin/pick_otus_through_otu_table.py", line 128, in main
    status_update_callback=status_update_callback)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/qiime/workflow.py", line 471, in run_pick_otus_through_otu_table
    close_logger_on_success=close_logger_on_success)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/qiime/workflow.py", line 135, in call_commands_serially
    raise WorkflowError, msg
qiime.workflow.WorkflowError: 

*** ERROR RAISED DURING STEP: Align sequences
Command run was:
 python /home/maasha/Install/QIIME1.6/bin/align_seqs.py -i TEST/otus/rep_set//seqs_rep_set.fasta -o TEST/otus/pynast_aligned_seqs 
Command returned exit status: 1
Stdout:

Stderr
Traceback (most recent call last):
  File "/home/maasha/Install/QIIME1.6/bin/align_seqs.py", line 205, in <module>
    main()
  File "/home/maasha/Install/QIIME1.6/bin/align_seqs.py", line 188, in main
    log_path=log_path,failure_path=failure_path)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/qiime/align_seqs.py", line 240, in __call__
    aligned=DenseAlignment)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/cogent/__init__.py", line 108, in LoadSeqs
    label_to_name=label_to_name, **constructor_kw)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/cogent/core/alignment.py", line 1957, in __init__
    super(DenseAlignment, self).__init__(*args, **kwargs)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/cogent/core/alignment.py", line 298, in __init__
    Alphabet=self.Alphabet)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/cogent/core/alignment.py", line 420, in _names_seqs_order
    seqs, names = conversion_f(data, Alphabet=Alphabet)
  File "/home/maasha/install/QIIME1.6/lib/python2.7/site-packages/cogent/core/alignment.py", line 1858, in aln_from_kv_pairs
    result = array(map(Alphabet.toIndices, seqs), array_type)
ValueError: setting an array element with a sequence.