Created
October 1, 2019 13:36
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library(cobs) | |
library(quantreg) | |
library(parallel) | |
library(gplots) | |
interpolate<-function(dat,curve){ | |
interpolate_points<-function(row,dat,curve){ | |
MY_X=dat[row,1] | |
MY_Y=dat[row,2] | |
VAL1=tail(which(curve[,1]<MY_X),1) | |
VAL2=VAL1+1 | |
X <- curve[c(VAL1,VAL2),1] | |
Y <- curve[c(VAL1,VAL2),2] | |
INTERP_Y=approx(X,Y,xout=MY_X)$y | |
INTERP_Y | |
} | |
res<-unlist(mclapply(seq(nrow(dat)),interpolate_points,dat=dat,curve=curve,mc.cores=8)) | |
res | |
} | |
################################################ | |
# 1Mbp bins | |
################################################ | |
x<-read.table("SRP199233.1e6_fmt.tsv",header=T,row.names=1) | |
x<-x[which(rowSums(x)>=10),] | |
x<-sweep(x, 2, colSums(x), FUN="/")*1000000 | |
mysd<-apply(x,1,sd) | |
mean<-apply(x,1,mean) | |
y<-data.frame(log10(mean),mysd/mean) | |
colnames(y)=c("logMean","cv") | |
Rbs.9 <- cobs(y$logMean,y$cv, nknots=20,constraint="none",tau=0.99) | |
Rbs.median <- cobs(y$logMean,y$cv,nknots=20,constraint="none",tau=0.5) | |
pred<-data.frame(predict(Rbs.9)) | |
y$interpolated<-interpolate(y,pred) | |
y$diff=y$cv-y$interpolated | |
yy <- y[which(y$diff>0.05),] | |
yy <- yy[order(-yy$diff),] | |
write.table(yy,file="SRP199233.1e6_regions.tsv") | |
png("plots/SRP199233.1e6_cv.png",height=960,width=960) | |
plot(y$logMean,y$cv,pch=18,cex=0.5,xlab="log10(mean)",ylab="CV") | |
lines(predict(Rbs.9), col = "red", lwd = 1.0) | |
lines(predict(Rbs.median), col = "blue", lwd = 1.0) | |
points(yy$logMean,yy$cv) | |
text(yy$logMean,yy$cv+0.02,labels=rownames(yy),cex=0.8) | |
dev.off() | |
png("plots/SRP199233.1e6_hm1.png",height=960,width=960) | |
my_palette <- colorRampPalette(c("blue", "white", "red"))(n = 25) | |
zz<-x[which(rownames(x) %in% rownames(yy)),] | |
heatmap.2(as.matrix(zz),margin=c(8, 22),cexRow=0.65,trace="none", | |
cexCol=0.8,col=my_palette,scale="row") | |
dev.off() | |
png("plots/SRP199233.1e6_hm2.png",height=960,width=960) | |
heatmap.2(cor(t(zz)),trace="none",scale="none",margins=c(12,12), | |
cexRow=0.8, cexCol=0.8) | |
dev.off() |
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