Roary plots

roary.ipynb

roary.py

#!/usr/bin/env python
# Copyright (C) <2015> EMBL-European Bioinformatics Institute

# This program is free software: you can redistribute it and/or
# modify it under the terms of the GNU General Public License as
# published by the Free Software Foundation, either version 3 of
# the License, or (at your option) any later version.

# This program is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
# GNU General Public License for more details.

# Neither the institution name nor the name roary_plots
# can be used to endorse or promote products derived from
# this software without prior written permission.
# For written permission, please contact <marco@ebi.ac.uk>.

# Products derived from this software may not be called roary_plots
# nor may roary_plots appear in their names without prior written
# permission of the developers. You should have received a copy
# of the GNU General Public License along with this program.
# If not, see <http://www.gnu.org/licenses/>.

__author__ = "Marco Galardini"
__version__ = '0.1.0'

def get_options():
    import argparse

    # create the top-level parser
    description = "Create plots from roary outputs"
    parser = argparse.ArgumentParser(description = description,
                                     prog = 'roary_plots.py')

    parser.add_argument('tree', action='store',
                        help='Tree Newick file')
    parser.add_argument('roary', action='store',
                        help='Roary gene presence/absence table')

    parser.add_argument('--version', action='version',
                         version='%(prog)s '+__version__)

    return parser.parse_args()

if __name__ == "__main__":
    options = get_options()

    import matplotlib.pyplot as plt
    import seaborn as sns

    sns.set_style('white')

    import os
    import pandas as pd
    import numpy as np
    from Bio import Phylo

    t = Phylo.read(options.tree, 'newick')

    # Max distance to create better plots
    mdist = max([t.distance(t.root, x) for x in t.get_terminals()])

    # Load roary
    roary = pd.read_table(options.roary,
                         sep=',',
                         low_memory=False)
    # Set index (group name)
    roary.set_index('Gene', inplace=True)
    # Drop the other info columns
    roary.drop(list(roary.columns[:10]), axis=1, inplace=True)

    # Transform it in a presence/absence matrix (1/0)
    roary.replace('.{2,100}', 1, regex=True, inplace=True)
    roary.replace(np.nan, 0, regex=True, inplace=True)

    # Sort the matrix by the sum of strains presence
    idx = roary.sum(axis=1).order(ascending=False).index
    roary_sorted = roary.ix[idx]

    # Pangenome frequency plot
    plt.figure(figsize=(7, 5))

    plt.hist(roary.sum(axis=1), roary.shape[1],
             histtype="stepfilled", alpha=.7)

    plt.xlabel('Number of genomes')
    plt.ylabel('Number of genes')

    sns.despine(left=True,
                bottom=True)
    plt.savefig('pangenome_frequency.png')
    plt.clf()

    # Sort the matrix according to tip labels in the tree
    roary_sorted = roary_sorted[[x.name for x in t.get_terminals()]]

    # Plot presence/absence matrix against the tree
    with sns.axes_style('whitegrid'):
        fig = plt.figure(figsize=(17, 10))

        ax1=plt.subplot2grid((1,40), (0, 10), colspan=30)
        a=ax1.matshow(roary_sorted.T, cmap=plt.cm.Blues,
                   vmin=0, vmax=1,
                   aspect='auto',
                   interpolation='none',
                    )
        ax1.set_yticks([])
        ax1.set_xticks([])
        ax1.axis('off')

        ax = fig.add_subplot(1,2,1)
        ax=plt.subplot2grid((1,40), (0, 0), colspan=10, axisbg='white')

        fig.subplots_adjust(wspace=0, hspace=0)

        ax1.set_title('Roary matrix\n(%d gene clusters)'%roary.shape[0])

        Phylo.draw(t, axes=ax, 
                   show_confidence=False,
                   label_func=lambda x: None,
                   xticks=([],), yticks=([],),
                   ylabel=('',), xlabel=('',),
                   xlim=(-0.01,mdist+0.01),
                   axis=('off',),
                   title=('parSNP tree\n(%d strains)'%roary.shape[1],),
                   do_show=False,
                  )
        plt.savefig('pangenome_matrix.png')
        plt.clf()

    # Plot the pangenome pie chart
    plt.figure(figsize=(10, 10))

    core = roary[roary.sum(axis=1) == roary.shape[1]].shape[0]
    softcore = roary[(roary.sum(axis=1) < roary.shape[1]) &
                     (roary.sum(axis=1) >= roary.shape[1]*0.95)].shape[0]
    shell = roary[(roary.sum(axis=1) < roary.shape[1]*0.95) &
                     (roary.sum(axis=1) >= roary.shape[1]*0.15)].shape[0]
    cloud = roary[roary.sum(axis=1) < roary.shape[1]*0.15].shape[0]

    total = roary.shape[0]

    def my_autopct(pct):
        val=int(pct*total/100.0)
        return '{v:d}'.format(v=val)

    a=plt.pie([core, softcore, shell, cloud],
          labels=['core\n(%d strains)'%roary.shape[1],
                  'soft-core\n(%d <= strains < %d)'%(roary.shape[1]*.95,
                                                     roary.shape[1]),
                  'shell\n(%d <= strains < %d)'%(roary.shape[1]*.15,
                                                 roary.shape[1]*.95),
                  'cloud\n(strains < %d)'%(roary.shape[1]*.15)],
          explode=[0.1, 0.05, 0.02, 0], radius=0.9,
          colors=[(0, 0, 1, float(x)/total) for x in (core, softcore, shell, cloud)],
          autopct=my_autopct)
    plt.savefig('pangenome_pie.png')
    plt.clf()