IJ-kaitei-11-hw1.js

//setTool("zoom");
run("Split Channels");
run("Gaussian Blur...", "sigma=1 stack");

//selectWindow("C2-NPC1.tif");
//selectWindow("C1-NPC1.tif");
run("Auto Threshold", "method=Li white stack use_stack_histogram");
run("Open", "stack");
run("Fill Holes", "stack");
run("Duplicate...", "title=C2-NPC1-1.tif duplicate range=1-15");
run("Erode", "stack");
run("Erode", "stack");
run("Erode", "stack");
selectWindow("C2-NPC1.tif");
run("Dilate", "stack");
run("Dilate", "stack");
run("Dilate", "stack");
imageCalculator("Subtract create stack", "C2-NPC1.tif","C2-NPC1-1.tif");
for (i = 0; i < nSlices; i++){
	run("Create Selection");
	run("Make Inverse");
	roiManager("Add");
	run("Select None");
	run("Next Slice [>]");
}
selectWindow("C1-NPC1.tif");
run("Bleach Correction", "correction=[Simple Ratio] background=9");
run("Set Measurements...", "area mean standard min integrated stack redirect=None decimal=5");
run("Clear Results");
roiManager("Select", newArray(0,1,2,3,4,5,6,7,8,9,10,11,12,13));
roiManager("Measure");
//run("Plot Results", "plot=[Imported Data] x-values=Slice y-values=Mean style=Line color=Red gridlines gridlines labels labels major major");